CN1727902A - Full-automatic urine analysis system - Google Patents
Full-automatic urine analysis system Download PDFInfo
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- CN1727902A CN1727902A CN 03152971 CN03152971A CN1727902A CN 1727902 A CN1727902 A CN 1727902A CN 03152971 CN03152971 CN 03152971 CN 03152971 A CN03152971 A CN 03152971A CN 1727902 A CN1727902 A CN 1727902A
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Abstract
A fully automatic analysis system of urine consists of a computer, sediment microscopic examination device controlled by computer, flow cell counting device , dry chemical analyzing device and integrated piping system for providing sample solution. It works as carrying out dry chemical analyzing and / or flow cell counting detection on urine sample, producing standard urine test report if detection result is negative and recording sample number and detection result if result is positive, sending positive sample to sediment microscopic examination device for producing out urine test report .
Description
Technical field
Invention relates to the technology that is used for urine is carried out check and analysis in a kind of medical field.
Technical background
Urinalysis is the analysis-by-synthesis of urine outward appearance, Neo-Confucianism, chemistry, microexamination, is that clinical examination three is big one of conventional, is widely used in clinically, and diagnosis, treatment and the curative effect monitoring of kidney and disease in the urological system had important value.At present domestic and foreign hospitals is generally used the urinalysis device to carry out routine urinalysis to detect, only can detect some biochemical component in the urine, as protein, sugar etc., and can not detect visible component in the urine, as red blood cell, leucocyte, cast, crystallization etc.And check that clinically the visible component in the urine all adopts microscopically manually to carry out analysis and distinguishing, be called urinary sediment microscopy (Examination of urinarysediments).
And traditional urinary sediment microscopy, owing to lack standardized device device and inspection means, human factor causes between the operator, lack comparability between the laboratory, result's difference is very big, the truth that can not reflect detection objectively, be unfavorable for that urine sediment analysis carries out quality control, draw result qualitative or that estimate and also often make the doctor be difficult to the patient is observed in diagnostic procedure dynamically, directly influence diagnostic value.
Along with Chinese clinical examination standard subcommittee (CCCLS) and U.S. clinical examination standard committee (NCCLS) integrate with, China has taken up to formulate the urinalysis levels of standards, with accuracy and the comparability that improves daily urinalysis in recent years.Accomplish the urinalysis process standardization, at first will make regularized operation, comprise standardization, the standardization of inspection method and the standardization of criterion etc. of urine sample collecting standardization, use equipment.Therefore, provide a kind of urinalysis standardization, normalized system equipment extremely urgent.
Summary of the invention
The present invention will overcome the many defectives that exist in the existing urinalysis technology, a kind of robotization, standardization, high-precision urine analysis system are provided, in conjunction with and be used in the advantage separately of check of chemical analysis, fluidic cell and urinary sediment microscopy, urine is implemented to analyze fast and accurately.
Summary of the invention
For achieving the above object, the present invention is by realizing by the following technical solutions: the full automatic urine analytic system, it is characterized in that: system comprises a computing machine and by this computer-controlled sediment microscopic inspection device, flow cytometry counting apparatus and dry analysis device, native system also comprises a feeding integrated pipeline system that is used to control and provide to said apparatus sample liquid.
System at first carries out the detection of dry analysis and/or flow cytometry to urine sample, and testing result is judged, the negative urine detection report that then directly generates standard of testing result; Testing result positive then device recording catalogue number(Cat.No.) and testing result; Same batch of sample is all over after the check of sieving of phase one, and undertaken centrifugally by automatic sampling device, and positive sample is delivered to the sediment microscopic inspection device, continues the examination of urinary sediment analysis and also draws urine detection report.
Described feeding integrated pipeline system accepts the control signal of computing machine and finishes to corresponding intrument to provide and carry correct type specimen, wherein specifically finish the check groove of crude urine being carried and is sprayed onto the dry analysis device, crude urine is carried and is arrived by high-pressure injection the cell detection passage of flow cytometry counting apparatus, with behind the sample liquid high speed centrifugation 0.2 milliliter of sediment, 0.2 milliliter of dyeing sediment or doubly amount dilution sediment be transported in the microscopy flowing counting pool, and finish the cleaning of sample channel.
System computer also is connected with result display apparatus, keyboard/mouse operating equipment and output device as a result.
As shown from the above technical solution, full automatic urine analytic system provided by the present invention is a urinalysis detection system of new generation, electricity, optics, fluid mechanics have wherein been adopted, utilize computer technology, electronic technology, Flow Cytometry, image processing technique, singlechip technology, stepper motor subdivide technology, photo etched mask technology, use fuzzy control principle, adopt unique power drive system, the quantitative counting pond, realized robotization, intellectuality, the quantification of fixture action, and the full-automation of microscope action.Adopt multimedia programming and database programming technology, realized the combination of image and database, and the inquiry of high capacity multi-medium data.Use VERTMENU control and video acquisition, image is handled.Adopt that medium filtering, profile extraction or value are separated etc., improved accuracy, the confidence level of cytological classification identification.
Summary of drawings
Fig. 1 is a theory diagram of the present invention;
Fig. 2 is a structure principle chart of the present invention.
Below in conjunction with accompanying drawing 1 and testing process principle of the present invention is further described.
The dry chemical means principle: dry type urinalysis device is to grow up on the basis of the various compositions of single chemistry examination band method mensuration urine.Past is to utilize visual inspection to try colored variation, compares with on-gauge plate and draws corresponding numerical value.Dry type urinalysis device then is to utilize sphere area to analyze instrument under the control of micro computer will try to be with the light that reflects to gather on the photoelectric detector, convert electric signal to through it, again through circuit and Computer Processing measure examination with on change in color carry out the stage quantitative test.Because dry type urinalysis device is finished above-mentioned measuring process automatically, therefore can obtain than the more accurate test result of naked eyes.The test strips (band) that dry type urinalysis device is commonly used is tens of centimeter length, on the wide plastic strip of 5mm every the indicator paper block of the wide square shape of the sticking lastblock 5mm of about 2mm, the corresponding measurement index of each indicator paper block, a blank block of not participating in reaction---compensation block is as urine background color, so that caused errors such as coloured urine and instrument variation are compensated in addition on test strips in addition.During test, the test strips after the urine immersion is placed on the testing jig of instrument, makes it be subjected to the scanning of optoelectronic scanning mechanism.The photoelectric detection mechanism top is the illuminator of a strip, and the light that illuminator sends can be radiated at by the light hole up and down of surface integral on the reagent piece of test strips, and the reagent piece then can reflex to a part of light in the integrating sphere.Because reagent piece color look and depth difference, the light intensity of its reflection is also different.For example, the content of certain chemical constitution is lower in the urine, and the color relation of its reagent corresponding piece is more shallow, the light intensity that it reflects; Otherwise, if content is higher, then Fan She light intensity a little less than.
The surface integral instrument has the effect of converging ray, the light that is reflected on the reagent piece is assembled the back by the surface integral instrument and is shone on the photoelectric detector by optical filter, photoelectric detector is converted to electric signal with light signal, electric signal send one to microprocessor system through amplifier and digital-to-analog conversion, microprocessor is obtained the reflectivity of the reflectivity of every kind of tested reagent piece and compensation block and is compared with the curve that exists reflectivity in the microcomputer and expression to analyze constituent concentration in advance, at last by semiquantitative grade symbol of printer output and concentration value.
The depth of reagent piece color is except that the difference with each tested composition and content becomes, and is also relevant with the color of urine itself.For eliminating this error, on test strips, be provided with blank block (compensation block), though it with tested composition change color does not take place, with the change color of urine.
Dry type urinalysis device adopts dual wavelength (by a branch of mensuration and a branch of reference light of optical filter) determination method at present.Instrument can calculate reflectivity R automatically by following formula.
The reflectivity of test paper
R test paper=Tm (reagent is to measuring the reflection strength of wavelength)/TS (reagent is to the reflection strength of reference wavelength) * 100%
Blank reflectivity
R blank=Cm (blank)/CS (blank reflection strength) * 100% to reference wavelength to measuring the reflection strength of wavelength
Total reflectivity R
R=R test paper/R blank=Tm CS/TS Cm * 100%
The fluidic cell principle of work: Flow Cytometry comprises liquid fluid system, optical system and electronic system.Cell in the sample to be tested transmits the zone of passing through laser radiation in the stream type cell device in order through liquid fluid system, the cell Stimulated Light excite the generation signal, accepted by signal receiver and amplify, these signals that amplified are the machine analyzing and processing as calculated, and shows intuitively with the form of chart; The signal that stream type cell device produces and analyzes mainly contains light scattering signal and fluorescent signal.The power of light scattering signal can reflect the degree etc. of size, form and the cytoplasmic granuleization of cell.According to the difference of luciferin, with the optical excitation of different wave length, launch the fluorescent of different wave length, can show different colors, these fluorescent signals just can reflect different characteristics of cell biology.
To make single cell suspension behind the cell dyeing to be measured.With certain pressure testing sample is pressed into flow chamber, not celliferous level pad under high pressure sprays from the sheath fluid pipe, sheath fluid tube inlet direction and testing sample stream are angled, like this, sheath fluid just can hold the sample flow at high speed, the a fluid stream of forming a circle, cell to be measured single file under the bag quilt of sheath fluid is arranged, successively by laser irradiation area (laser beam of fluid column and incident intersects vertically and a little is called detection zone), be subjected to send fluorescent of all kinds after the strong laser radiation by the cell of fluorescence staining, produce light scattering simultaneously.The collection of fluorescent signal can be placed optical system (lens, Guang Lan and filter disc etc.) in order to collect the fluorescent signal in 90 vertical degree directions of laser beam, invests detecting device.The fluorescent detecting device is a photomultiplier, and light scattering detector is a photodiode, is used for collecting forward angle light scatter light.Light scattering signal is surveyed deeply at the little angle of forward direction (2-10 degree), and this measurement claims " forward angle light scatter " or " forward scattering ".The light scattering signal of Chan Shenging has reflected the size of cell volume basically in this case.Fluorescent photomultiplier and forward light electric diode that fluorescent signal that cell sends and light scattering signal are an angle of 90 degrees direction simultaneously receive, be converted to electronic signal input multiple tracks (1024 road) pulse analysis device after being exaggerated integration, and on computer screen, show with one dimension histogram, two-dimensional bitmap or three-dimensional picture.Or figure and data directly imported the computing machine of online special use, or deposit disk in order to analyzing.Computing machine is surveyed data computation with institute fast and accurately and is come out, and in conjunction with multi parameter analysis, thereby has realized cell quantitative.
Lamina flow principle: Re>2300 are that turbulent flow Re≤2300 are current stabilization
Re=dρν/η
D: caliber
ρ: fluid density
ν: flow rate of liquid
η: coefficient of viscosity
Bemoulli law: S1V1=S2V2
S, V are respectively the sectional area of pipeline and the flow velocity of liquid
Sediment microscopic inspection principle: dose and a series of auto-programmings (to the doubly amount dilution automatically of high density sample) such as dyed blended of realizing arena sample and dyeing by system controlled by computer.Specimen transfer after mixing, dyeing to special optics sediment constant flow counting chamber, can be obtained colored clearly arena image by built-in figure case phase microscope camera system on indicator screen.Adopt that medium filtering, profile extraction or value are separated etc., carry out cytological classification identification, and visible component in the arena is carried out quantitative Analysis.Realized the quantitative test of urinary formed element.
The above has introduced relevant sediment microscopic inspection device, flow cytometry counting apparatus and dry analysis device principle of work separately, and core of the present invention is they are organically combined.At first sediment microscopic inspection device, flow cytometry counting apparatus and dry analysis device are separate equipment separately, can detect urine, and draw corresponding conclusion.
Dry chemical check speed is very fast, can reach 20 seconds/sample, carries out qualitative detection with it; Fluidic cell check velocity ratio is very fast, can reach 50 seconds/sample, can write down red blood cell, leucocyte content in every microlitre urine exactly, but they all can't discern other visible component, and two kinds of checks all are a kind of sieving detection; And the urinary sediment microscopy that the sediment microscopic inspection device is finished is that urine image under mirror is carried out inspection process, utilize shooting and computer image processing in conjunction with the Computer Automatic Recognition technology, can write down the various visible components in the urine sample, its testing result is the most comprehensive, even but it is making full use of on the basis of various image technologies, detection speed is the slowest a kind of by contrast, can only reach 120 seconds/sample.The present invention has utilized the advantage separately of above-mentioned each apparatus just, and it is combined.System disclosed in this invention at first urinates the detection of dry chemical and fluidic cell simultaneously to urine sample, and the result who draws judged, the method of judging is the binary data that utilizes in the computing machine, if negative sample, just generate the urine detection report of a standard at once, if but positive sample, device is record mark this shop and testing result automatically, be all over after the check of sieving of phase one Deng 20 sample, undertaken centrifugal by automatic sampling device, and positive sample delivered to microscopic examination device, continue to finish the examination of urinary sediment analysis by this device, and provide urine detection report.
The structure and the principle of feeding integrated pipeline system among the present invention are described below in conjunction with Fig. 2: described feeding integrated pipeline system is by mixer 16, pump, valve and pipeline constitute, wherein sample liquid is successively via quantitative peristaltic pump 4, valve 11 links to each other with mixer 16, dyeing liquor is successively via quantitative peristaltic pump 3, valve 10 links to each other with mixer 16, dilution is successively via quantitative peristaltic pump 2, valve 9 links to each other with mixer 16, cleaning fluid is successively via quantitative peristaltic pump 1, valve 8 links to each other with mixer 16, the output terminal of mixer 16 respectively with the check groove of dry analysis device, the cell detection passage of flow cytometry counting apparatus and microscopy flowing counting pool are communicated with.
Claims (9)
1, a kind of full automatic urine analytic system, it is characterized in that: system comprises a computing machine and by this computer-controlled sediment microscopic inspection device, flow cytometry counting apparatus and dry analysis device, native system also comprises the feeding integrated pipeline system and the automatic sampling device that are used to control and provide to said apparatus sample liquid.
2, full automatic urine analytic system according to claim 1, it is characterized in that: system at first carries out the detection of dry analysis and/or flow cytometry to urine sample, and testing result judged the negative urine detection report that then directly generates standard of testing result; Testing result positive then device recording catalogue number(Cat.No.) and testing result; Same batch of sample is all over after the check of sieving of phase one, and undertaken centrifugally by automatic sampling device, and positive sample is delivered to the sediment microscopic inspection device, continues the examination of urinary sediment analysis and also draws urine detection report.
3, full automatic urine analytic system according to claim 1 and 2, it is characterized in that: described feeding integrated pipeline system accepts the control signal of computing machine and finishes to corresponding intrument to provide and carry correct type specimen, wherein specifically finish the check groove of crude urine being carried and is sprayed onto the dry analysis device, crude urine is carried and is arrived by high-pressure injection the cell detection passage of flow cytometry counting apparatus, with behind the sample liquid high speed centrifugation 0.2 milliliter of sediment, 0.2 milliliter dyeing sediment or doubly amount dilution sediment be transported in the microscopy flowing counting pool, and finish the cleaning of sample channel.
4, full automatic urine analytic system according to claim 1 and 2 is characterized in that: computing machine also is connected with result display apparatus, keyboard/mouse operating equipment and output device as a result.
5, full automatic urine analytic system according to claim 1 and 2, it is characterized in that: described feeding integrated pipeline system is by mixer (16), pump, valve and pipeline constitute, wherein sample liquid is successively via quantitative peristaltic pump (4), valve (11) links to each other with mixer (16), dyeing liquor is successively via quantitative peristaltic pump (3), valve (10) links to each other with mixer (16), dilution is successively via quantitative peristaltic pump (2), valve (9) links to each other with mixer (16), cleaning fluid is successively via quantitative peristaltic pump (1), valve (8) links to each other with mixer (16), the output terminal of mixer (16) respectively with the check groove of dry analysis device, the cell detection passage of flow cytometry counting apparatus and microscopy flowing counting pool are communicated with.
6, full automatic urine analytic system according to claim 5, it is characterized in that: the mixer (16) in the described feeding integrated pipeline system is carried crude urine and be sprayed onto on the check groove of dry analysis device via valve (15), high-pressure pump (7) successively, is provided with pressure transducer (17) between the check groove of high-pressure pump (7) and dry analysis device.
7, full automatic urine analytic system according to claim 5, it is characterized in that: the mixer (16) in the described feeding integrated pipeline system is ejected into the cell detection passage of flow cytometry counting apparatus via valve (14), high-pressure pump (6) with crude urine successively, is provided with liquid level sensor (18) between the cell detection passage of high-pressure pump (6) and flow cytometry counting apparatus.
8, full automatic urine analytic system according to claim 5 is characterized in that: the mixer (16) in the described feeding integrated pipeline system successively via valve (13), two-way peristaltic pump (5) with sample liquid behind high speed centrifugation 0.2 milliliter of sediment, 0.2 milliliter of dyeing sediment or doubly amount dilution sediment be transported in the microscopy flowing counting pool.
9, full automatic urine analytic system according to claim 5, it is characterized in that: the mixer (16) in the described feeding integrated pipeline system takes back the waste collection container through valve (12), and waste liquid takes back the waste collection container through pipeline in the waste liquid of the cell detection passage of described flow cytometry counting apparatus and the microscopy flowing counting pool.
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CN101852715A (en) * | 2009-03-30 | 2010-10-06 | 希森美康株式会社 | Urine sample analyzer |
US20110022327A1 (en) * | 2009-07-24 | 2011-01-27 | Mark Busenhart | Urine work area manager for a urine work area |
CN102346181A (en) * | 2010-08-03 | 2012-02-08 | 希森美康株式会社 | Urine sample testing apparatus and apparatus for processing measurement result of urine sample |
CN102393705A (en) * | 2011-10-18 | 2012-03-28 | 长沙高新技术产业开发区爱威科技实业有限公司 | Sample formed component analyzer automatic detection control apparatus and control method thereof |
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US20110022327A1 (en) * | 2009-07-24 | 2011-01-27 | Mark Busenhart | Urine work area manager for a urine work area |
CN101968493A (en) * | 2009-07-24 | 2011-02-09 | 霍夫曼-拉罗奇有限公司 | A urine work area manager and a urine work area |
US9443058B2 (en) * | 2009-07-24 | 2016-09-13 | Roche Diagnostics Operations, Inc. | Urine work area manager for a urine work area |
CN101968493B (en) * | 2009-07-24 | 2014-11-19 | 霍夫曼-拉罗奇有限公司 | A urine work area managing method and a urine work area manager |
CN102346181A (en) * | 2010-08-03 | 2012-02-08 | 希森美康株式会社 | Urine sample testing apparatus and apparatus for processing measurement result of urine sample |
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CN103994974A (en) * | 2014-06-11 | 2014-08-20 | 长春理工大学 | Dry type biochemical analyzer |
CN103994974B (en) * | 2014-06-11 | 2017-02-22 | 长春理工大学 | Dry type biochemical analyzer |
CN104655862B (en) * | 2015-01-19 | 2016-04-06 | 路建磊 | Urine collecting analytical equipment |
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US10585032B2 (en) | 2015-03-31 | 2020-03-10 | Sysmex Corporation | Urine analysis system, image capturing apparatus, urine analysis method |
CN104819981A (en) * | 2015-05-16 | 2015-08-05 | 黄友锋 | Urine component measuring instrument |
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CN109612911A (en) * | 2018-12-26 | 2019-04-12 | 深圳天依生命健康科技有限公司 | Full-automatic spermatoblast detector |
CN113391082A (en) * | 2021-06-25 | 2021-09-14 | 湖南友哲科技有限公司 | Full-automatic comprehensive analysis all-in-one machine for urine and full-automatic urine detection and analysis system |
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