CN115044454A - Nucleic acid extraction apparatus - Google Patents
Nucleic acid extraction apparatus Download PDFInfo
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- CN115044454A CN115044454A CN202210762189.2A CN202210762189A CN115044454A CN 115044454 A CN115044454 A CN 115044454A CN 202210762189 A CN202210762189 A CN 202210762189A CN 115044454 A CN115044454 A CN 115044454A
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Abstract
The invention discloses nucleic acid extraction equipment, which comprises a main body frame, and a positive pressure platform, a reagent platform, a membrane column platform, a collection platform, a transverse driving assembly, a vertical driving assembly and an air pump assembly which are arranged on the main body frame; the positive pressure platform, the reagent platform, the membrane column platform and the collection platform are arranged from top to bottom; the positive pressure platform moves vertically on the main body frame and is provided with a plurality of air blowing joints; the reagent platform transversely moves on the main body frame and is used for bearing the reagent container; the membrane column platform is used for bearing a sample container; the collecting platform is transversely moved on the main body frame and is used for bearing the collecting container; the transverse driving component respectively drives the reagent platform and the collecting platform; the vertical driving assembly drives the positive pressure platform; the air pump assembly is connected with the air blowing joint of the positive pressure platform and used for pushing liquid in the container to drip downwards. The invention can realize rapid and large-batch automatic extraction of nucleic acid.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction automation, in particular to nucleic acid extraction equipment.
Background
In recent years, the technology of nucleic acid extraction has been developed rapidly, and various technologies and equipments for nucleic acid extraction have been developed. With the increase of the sample size, the demand of large hospitals and nucleic acid detection institutions for multi-channel and highly automated nucleic acid extraction instruments is urgent.
Because the magnetic bead method can realize automatic extraction of nucleic acid, the method is widely applied to the existing nucleic acid extraction equipment. Generally, the extraction steps for extracting nucleic acid by the magnetic bead method mainly comprise: cracking; combining; thirdly, rinsing; and fourthly, elution. The specific description is as follows: firstly, releasing DNA/RNA from cells or tissues under the action of a lysate; the superparamagnetic silicon oxide nanometer magnetic beads subjected to surface modification are specifically combined with the released DNA/RNA to form a nucleic acid-magnetic bead compound; thirdly, adding rinsing liquid into the nucleic acid-magnetic bead compound to wash away impurities such as non-specifically adsorbed protein, polysaccharide and the like; and fourthly, separating the nucleic acid-magnetic bead compound with the impurities removed from the magnetic beads in the eluent to finally obtain the nucleic acid substance to be extracted. If the steps of cracking, combining, rinsing and eluting are not mixed uniformly in the nucleic acid extraction process, the reagent is not fully contacted with the sample, the intracellular nucleic acid is not completely released, the magnetic beads are easy to settle, and the extraction efficiency is greatly reduced, so the nucleic acid extraction equipment adopting the magnetic bead method must be provided with an oscillating mechanism so as to fully mix the extraction steps, thereby the contact speed of the reagent and the sample is increased, and the combination or separation speed of the magnetic beads and the nucleic acid is increased.
After the magnetic bead method nucleic acid extraction equipment adopts the oscillation mechanism, although the mixing uniformity of each extraction step can be ensured, the nucleic acid extraction efficiency of the equipment is limited, and the magnetic bead method nucleic acid extraction equipment cannot be applied to occasions needing rapid and large-batch extraction of nucleic acid.
Disclosure of Invention
The invention aims to provide nucleic acid extraction equipment which can realize rapid and large-batch automatic extraction of nucleic acid.
In order to achieve the above purpose, the solution of the invention is:
a nucleic acid extraction device comprises a main body frame, and a positive pressure platform, a reagent platform, a membrane column platform, a collection platform, a transverse driving assembly, a vertical driving assembly and a pneumatic pump assembly which are arranged on the main body frame; the positive pressure platform, the reagent platform, the membrane column platform and the collection platform are sequentially arranged from top to bottom; the positive pressure platform vertically moves on the main body frame, and a plurality of air blowing joints are arranged on the positive pressure platform; the reagent platform moves transversely on the main body frame and is used for bearing a reagent container; the membrane column platform is used for bearing a sample container; the collecting platform moves transversely on the main body frame and is used for bearing a collecting container; the transverse driving assembly drives the reagent platform and the collection platform respectively so as to align the reagent container with the sample container and align the collection container with the sample container; the vertical driving assembly drives the positive pressure platform to enable the reagent container, the sample container and the collection container to be connected in sequence after being aligned; the air pump assembly is connected with the air blowing joint of the positive pressure platform and used for pushing liquid in the container to drip downwards.
The main body frame comprises a bottom plate, a rear plate and two side plates, wherein the rear plate and the two side plates are vertically connected to the bottom plate; the side surfaces of the two side plates are provided with a first transverse guide rail and a second transverse guide rail which are parallel to each other, the rear plate is provided with a vertical guide rail, the two ends of the reagent platform are movably matched above the two side plates and are respectively in sliding connection fit with the first transverse guide rails on the two sides, the two ends of the collecting platform are movably matched on the two side plates and are respectively in sliding connection fit with the second transverse guide rails on the two sides, and the positive pressure platform is in sliding connection fit with the vertical guide rails.
And the side plate is provided with a strip hole for the end part of the collecting platform to penetrate out and move.
The transverse driving assembly comprises two groups of first driving devices, a synchronous shaft, a first synchronous belt, an idler wheel and a second synchronous belt; the first driving device and the synchronizing shaft are both arranged on the back surface of the rear plate and are in transmission connection through the first synchronizing belt, and the synchronizing shaft is parallel to the bottom plate; the idler wheel is in running fit with the side face of the side plate and is in transmission connection with the synchronous shaft through the second synchronous belt; and the reagent platform and the collection platform are respectively connected with the two groups of second synchronous belts.
The first driving device is a stepping motor.
The positive pressure platform comprises the air blowing joint, an air blowing cross rod, an air blowing transverse plate and an air blowing vertical plate; the air blowing transverse rod and the air blowing transverse plate are arranged in parallel up and down, and two ends of the air blowing transverse rod and the air blowing transverse plate are respectively and vertically connected with the upper end and the lower end of the air blowing vertical plate; the blowing cross rod is in transmission connection with the vertical driving assembly; the air blowing joints are arranged on the air blowing transverse plate along a straight line.
The reagent platform comprises a first placing frame, a first connecting block and a pressing strip; the first placing frame is used for bearing a reagent container and is in sliding connection and matching with the main body frame through first connecting blocks at two ends; the pressing strip is detachably matched on the first placing frame and used for fixing the reagent container.
The reagent platform further comprises a first bearing, a first lifting shaft and a first spring; the first bearing is arranged at the end part of the first placing frame; the first lifting shaft is movably arranged in the first bearing in a penetrating way and is vertically connected with the first connecting block; the first spring is disposed between the first bearing and the first connecting block.
The membrane column platform comprises a second placing frame and a limiting block; the second placing rack is used for bearing a sample container and is installed on the main body frame through limiting blocks at two ends, and limiting grooves for the limiting blocks to be embedded are formed in the main body frame.
The membrane column platform further comprises a second bearing, a second lifting shaft and a second spring; a movable groove is formed in the side face of the limiting block; the second bearing is arranged at the end part of the second placing frame; the second lifting shaft movably penetrates through the second bearing and is vertically connected with two ends of the movable groove; the second spring is disposed between the second bearing and a lower end of the movable groove.
The collecting platform comprises a third placing frame and a second connecting block; the third placing frame is used for bearing the collecting container and is in sliding connection and matching with the main body frame through second connecting blocks at two ends.
The vertical driving assembly comprises a second driving device, a lifting rod and a fixed support; the second driving device is installed on the main body frame through the fixed support and drives the lifting rod to vertically extend and retract; the lower end of the lifting rod is connected with the positive pressure platform.
The second driving device is a screw rod motor.
The air pump assembly comprises an electromagnetic valve and an air pump; the electromagnetic valve is provided with an inlet and a plurality of outlets; the output end of the air pump is communicated to the inlet of the electromagnetic valve, and the outlet of the electromagnetic valve is respectively communicated to each air blowing joint.
The air pump assembly further comprises a flow divider, and a pressure sensor and a flow speed regulating valve which are arranged on the flow divider; the output end of the air pump is communicated with the input end of the flow divider; the inlet of the electromagnetic valve is communicated with the output end of the shunt; the pressure sensor is used for detecting the gas pressure in the flow divider; the flow rate regulating valve is used for regulating the gas pressure in the flow divider.
The nucleic acid extraction equipment also comprises a shell which covers the outer surface of the main body frame; the shell comprises a shell body and a turnover cover which is in pivot joint with the shell body.
After the technical scheme is adopted, the air pump assembly supplies air to the air blowing joint of the positive pressure platform, and can push liquid in each container to flow in an air pressure mode, so that each step of nucleic acid extraction is completed in the moving process of the positive pressure platform, the reagent platform and the collection platform, and nucleic acid extraction is realized in a layered acquisition mode after nucleic acid precipitation; the air pump assembly can blow air to a plurality of air blowing joints simultaneously, so that a plurality of samples are processed simultaneously in the same step, the processing efficiency of each step is guaranteed, and the nucleic acid can be extracted rapidly and automatically in large batch.
Drawings
FIG. 1 is a perspective view of an embodiment of the present invention;
FIG. 2 is a perspective view I (with the housing portion hidden) of a portion of the structure of an embodiment of the present invention;
FIG. 3 is a second perspective view of a portion of the structure of the present invention;
FIG. 4 is a first exploded view of a portion of the structure of an embodiment of the present invention;
FIG. 5 is a second exploded view of a portion of the structure of the present invention;
FIG. 6 is a perspective view of a body frame according to an embodiment of the present invention;
FIG. 7 is a perspective view of a positive pressure platen according to an embodiment of the present invention;
FIG. 8 is a perspective view of a reagent platform according to an embodiment of the present invention;
FIG. 9 is a perspective view of a membrane column platform according to an embodiment of the present invention;
FIG. 10 is a perspective view of a collection platform according to an embodiment of the present invention;
FIG. 11 is a perspective view of a lateral drive assembly in accordance with an embodiment of the present invention;
FIG. 12 is a perspective view of a vertical drive assembly according to an embodiment of the present invention;
FIG. 13 is a perspective view of an air pump assembly in accordance with an embodiment of the present invention;
FIG. 14 is a perspective view of a sensor assembly according to an embodiment of the present invention;
FIG. 15 is a perspective view of a reagent vessel according to an embodiment of the present invention;
FIG. 16 is a perspective view of a sample container according to an embodiment of the present invention;
FIG. 17 is a perspective view of a collection container according to an embodiment of the present invention;
the reference numbers illustrate:
1- -a main body frame; 11-a bottom plate; 12- -a back plate;
13- - -a side panel; 131- -elongated hole; 132- -a retaining groove;
14- -a first transverse rail; 15-a second transverse guide rail; 16-vertical guide rail;
2- -a positive pressure platen; 21- -blow joint; 22- -a blow beam;
23- -blowing transverse plate; 24- -blow riser; 3- -reagent platform;
31- - -a first rack; 32-a first connecting block; 33- -a hold-down bar;
34- -a first bearing; 35- -a first lift shaft; 36- -a first spring;
4- -a membrane column platform; 41- - -a second rack; 42- - -a stopper;
421- -active slot; 43- -a second bearing; 44- -a second lifting shaft;
45- -a second spring; 5- -a collection platform; 51- -third rack;
52- -a second connection block; 6- -a transverse drive assembly; 61- -a first drive;
62- -a synchronizing shaft; 63- - -a first synchronization belt; 64- -an idler pulley;
65- -a second synchronous belt; 66- -a bearing seat; 7-a vertical drive assembly;
71-a second drive; 72- -a lifter bar; 73- -fixed support;
8- -an air pump assembly; 81- -solenoid valve; 811- -inlet;
812- -an outlet; 82- - -a miniature air pump; 83- -a flow divider;
84- -a pressure sensor; 85- -flow rate regulating valve; 9- -a sensor assembly;
10- - -a housing; 101-a housing; 102- -flip cover;
103- -a touch screen; 20- -photoelectric switch; 30- -a reagent container;
301- -a cavity; 40- -sample container; 50- -a collection container;
501- -a nucleic acid container; 502- -waste container; 503- -a cover;
60- -silica gel plug.
Detailed Description
In order to further explain the technical solution of the present invention, the present invention is explained in detail by the following specific examples.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the embodiments of the present invention, it should be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings, or the orientations or positional relationships that the products of the present invention are usually placed in when in use, or the orientations or positional relationships that are conventionally understood by those skilled in the art, and are only used for the convenience of describing the embodiments of the present invention, but do not indicate or imply that the indicated devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and therefore, should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to a number of indicated technical features. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the description of the embodiments of the present invention, it should be further noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may include, for example, a fixed connection, a detachable connection, or an integral connection; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature "on," "above" and "over" the second feature may include the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
The following disclosure provides many different embodiments or examples for implementing different features of the invention. To simplify the disclosure of the present invention, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, such repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed.
In addition, the present invention provides examples of various specific processes and materials, but one of ordinary skill in the art may recognize applications of other processes and/or use of other materials.
Referring to fig. 1 to 17, the present invention discloses a nucleic acid extraction apparatus, comprising a main frame 1, and a positive pressure platform 2, a reagent platform 3, a membrane column platform 4, a collection platform 5, a transverse driving assembly 6, a vertical driving assembly 7 and an air pump assembly 8 which are mounted on the main frame 1;
the positive pressure platform 2, the reagent platform 3, the membrane column platform 4 and the collection platform 5 are sequentially arranged from top to bottom;
the positive pressure platform 2 moves vertically on the main body frame 1, and a plurality of air blowing joints 21 are arranged on the positive pressure platform;
the reagent platform 3 moves transversely on the main body frame 1 and is used for bearing the reagent container 30;
the membrane column platform 4 is used for carrying the sample container 40;
the collecting platform 5 moves transversely on the main body frame 1 and is used for carrying the collecting container 50;
the lateral drive assembly 6 drives the reagent platform 3 and the collection platform 5, respectively, to align the reagent container 30 with the sample container 40 and the collection container 50 with the sample container 40;
the vertical driving assembly 7 drives the positive pressure platform 2 to enable the reagent container 30, the sample container 40 and the collection container 50 to be connected in sequence after being aligned;
the air pump assembly 8 is connected with the air blowing joint 21 of the positive pressure platform 2 and is used for pushing the liquid in the container to drip downwards. (the liquid may be one of a sample, a reagent, a mixture, a nucleic acid, and a waste liquid)
Specific embodiments of the invention are shown below:
referring to fig. 6, there is shown an embodiment of the main body frame 1 of the present invention.
The main body frame 1 includes a bottom plate 11, and a rear plate 12 and two side plates 13 vertically connected to the bottom plate 11, and rear ends of the side plates 13 are vertically connected to the rear plate 12. The side surfaces of the two side plates 13 are provided with a first transverse guide rail 14 and a second transverse guide rail 15 which are parallel to each other, the rear plate 12 is provided with a vertical guide rail 16, the two ends of the reagent platform 3 are movably matched above the two side plates 13 and are respectively in sliding connection and matching with the first transverse guide rails 14 on the two sides, the two ends of the collecting platform 5 are movably matched on the two side plates 13 and are respectively in sliding connection and matching with the second transverse guide rails 15 on the two sides, and the positive pressure platform 2 is in sliding connection and matching with the vertical guide rails 16.
Further, the side plate 13 is provided with a long hole 131 for the end of the collecting platform 5 to penetrate and move, so that the first transverse guide rail 14 and the second transverse guide rail 15 can be arranged on the outer side surface of the side plate 13 so as to be assembled with the transverse driving assembly 6, and the structural layout is more reasonable.
Meanwhile, the first transverse guide rail 14, the second transverse guide rail 15 and the vertical guide rail 16 are all sliding connection devices composed of linear rails and sliders.
Referring to fig. 7, a positive pressure platen 2 according to an embodiment of the present invention is shown.
The positive pressure platform 2 comprises the air blowing joint 21, an air blowing cross rod 22, an air blowing transverse plate 23 and an air blowing vertical plate 24; the air blowing cross rod 22 and the air blowing transverse plate 23 are arranged in parallel up and down, and two ends of the air blowing cross rod 22 and the air blowing transverse plate 23 are respectively and vertically connected with the upper end and the lower end of the air blowing vertical plate 24 to form a complete frame structure; the blowing cross rod 22 is in transmission connection with the vertical driving assembly 7; the air blowing joints 21 are arranged on the air blowing cross plate 23 along a straight line.
Referring to fig. 8, a specific embodiment of the reagent platform 3 of the present invention is shown.
The reagent platform 3 comprises a first placing frame 31, a first connecting block 32 and a pressing strip 33; the first placing frame 31 is used for carrying the reagent container 30, and is in sliding fit with the main body frame 1 through the first connecting blocks 32 at the two ends, that is, the first connecting blocks 32 are installed on the sliding blocks of the first transverse guide rail 14, so that the sliding fit of the reagent platform 3 and the main body frame 1 is realized; the pressing bar 33 is detachably fitted on the first rack 31 for fixing the reagent vessel 30.
Further, the reagent platform 3 further includes a first bearing 34, a first lifting shaft 35, and a first spring 36; the first bearing 34 is installed at the end of the first placing frame 31; the first lifting shaft 35 is movably arranged through the first bearing 34 and is vertically connected with the first connecting block 32; the first spring 36 is disposed between the first bearing 34 and the first connecting block 32. Under the action of the elastic force of the first spring 36, the first placing frame 31 is lifted upwards, so that the lower end of the reagent container 30 is higher than the upper end of the sample container 40, and the reagent container 30 and the sample container 40 cannot collide when the reagent platform 3 moves back and forth on the main body frame 1; when the positive pressure platform 2 is pressed down, the reagent platform 3 is pressed down a short distance, so that the lower end of the reagent container 30 is inserted into the upper end of the sample container 40, the reagent in the reagent container 30 is squeezed into the sample container 40 when the air pump assembly 8 blows air conveniently, and then after the positive pressure platform 2 rises, the reagent platform 3 resets upwards under the action of the first spring 36. At the same time, the first spring 36 also acts as a buffer in the reagent platform 3, avoiding the reagent container 30 from being damaged by excessive pressure of the positive pressure platform 2.
Referring to fig. 9, there is shown an embodiment of the membrane column platform 4 of the present invention.
The membrane column platform 4 comprises a second placing frame 41 and a limiting block 42; the second rack 41 is used for carrying the sample container 40, and is installed on the main body frame 1 through the limiting blocks 42 at two ends, and the main body frame 1 (i.e. the side plate 13) is provided with a limiting groove 132 for the limiting block 42 to be embedded.
Further, the film column platform 4 further comprises a second bearing 43, a second lifting shaft 44 and a second spring 45; the side surface of the limiting block 42 is provided with a movable groove 421; the second bearing 43 is installed at the end of the second placing frame 41; the second lifting shaft 44 is movably arranged through the second bearing 43 and vertically connected with two ends of the movable groove 421; the second spring 45 is disposed between the second bearing 43 and the lower end of the movable groove 421. Under the action of the elastic force of the second spring 45, the second placing frame 41 is jacked upwards, so that the lower end of the sample container 40 is higher than the upper end of the collecting container 50, and the collecting container 50 and the sample container 40 cannot collide when the collecting platform 5 moves back and forth on the main body frame 1; when the positive pressure platform 2 is pressed down, the membrane column platform 4 is pressed down for a short distance, so that the lower end of the sample container 40 is inserted into the upper end of the collecting container 50, liquid (nucleic acid or waste liquid) of the sample container 40 is squeezed into the collecting container 50 when the air pump assembly 8 blows air conveniently, and then after the positive pressure platform 2 rises, the membrane column platform 4 is reset upwards under the action of the second spring 45. Meanwhile, the second spring 45 also plays a role of buffering in the reagent platform 3, so that the sample container 40 is prevented from being damaged due to excessive pressure of the positive pressure platform 2.
Referring to fig. 10, a collection platform 5 according to an embodiment of the present invention is shown.
The collecting platform 5 comprises a third placing frame 51 and a second connecting block 52; the third rack 51 is used for carrying the collecting container 50, and penetrates through the elongated hole 131 to be in sliding fit with the main body frame 1 through the second connecting blocks 52 at two ends, that is, the second connecting blocks 52 are installed on the sliding blocks of the second transverse guide rail 15, so as to realize the sliding fit of the collecting platform 5 and the main body frame 1.
Referring to fig. 11, there is shown an embodiment of the transverse drive assembly 6 of the present invention.
The transverse driving assembly 6 comprises two groups of first driving devices 61, a synchronous shaft 62, a first synchronous belt 63, an idle wheel 64 and a second synchronous belt 65; the first driving device 61 and the synchronizing shaft 62 are both arranged on the back surface of the back plate 12 and are in transmission connection through a first synchronizing belt 63, and the synchronizing shaft 62 is parallel to the bottom plate 11; the idle pulley 64 is in running fit with the side surface of the side plate 13 and is in transmission connection with the synchronous shaft 62 through a second synchronous belt 65; the reagent platform 3 (i.e., the first connecting block 32) and the collecting platform 5 (i.e., the second connecting block 52) are respectively connected to the two sets of second timing belts 65, so that when the two sets of first driving devices 61 operate, the reagent platform 3 and the collecting platform 5 are driven to move on the first transverse guide rail 14 and the second transverse guide rail 15 sequentially through power transmission of the first timing belts 63, the synchronizing shafts 62 and the second timing belts 65. The transverse driving assembly 6 with the structure can optimally utilize the installation space on the main body frame 1, and the power of the first driving device 61 on the back surface of the main body frame 1 is turned and transmitted to the side surface of the main body frame 1, so that the whole structure of the device is more compact while the power stability of the reagent platform 3/the collecting platform 5 is ensured, and the volume of the product is reduced.
Further, the synchronizing shaft 62 is mounted on the back surface of the rear plate 12 via a bearing housing 66.
Meanwhile, the first driving device 61 is a stepping motor.
Referring to fig. 12, there is shown an embodiment of the vertical drive assembly 7 of the present invention.
The vertical driving assembly 7 comprises a second driving device 71, a lifting rod 72 and a fixed bracket 73; the second driving device 71 is mounted on the main body frame 1 through a fixing bracket 73 and drives the lifting rod 72 to perform vertical telescopic movement; the lower end of the lifting rod 72 is connected to the positive pressure platform 2 (i.e., the air-blowing crossbar 22 described above).
Further, the second driving device 71 is a screw motor.
Referring to fig. 13, an embodiment of the air pump assembly 8 of the present invention is shown.
The air pump assembly 8 comprises an electromagnetic valve 81 and a micro air pump 82; the solenoid valve 81 is provided with an inlet 811 and a plurality of outlets 812; the output end of the micro air pump 82 is connected to the inlet 811 of the electromagnetic valve 81, and the outlet 812 of the electromagnetic valve 81 is connected to each air blowing joint 21. The air output from the micro air pump 82 can be supplied to each of the air-blowing joints 21 by the control of the electromagnetic valve 81.
Further, the air pump assembly 8 further includes a flow divider 83, and a pressure sensor 84 and a flow rate regulating valve 85 disposed on the flow divider 83; the output end of the micro air pump 82 is communicated with the input end of the shunt 83; an inlet 811 of the electromagnetic valve 81 is communicated with an output end of the flow divider 83; a pressure sensor 84 for sensing the pressure of the gas in the flow divider 83, which may alert the operator of the apparatus at low or high pressures; the flow rate regulating valve 85 is used to regulate the gas pressure in the flow divider 83 to ensure that the pressure value is within a preset range.
In addition, the present invention also includes a sensor assembly 9 as shown in FIG. 14; the sensor assembly 9 is disposed right below the initial position of the reagent platform 3, and is used for detecting whether the corresponding position has the collection container 50, and sensing that a certain channel has the collection container 50, the control system of the device can recognize and conduct the valve port of the electromagnetic valve 81 corresponding to the channel.
The invention also comprises a shell 10 which covers the outer surface of the main body frame 1; the housing 10 includes a housing 101 and a flip 102 pivotally coupled to the housing 101. In use of the device, the operator may directly open the flap 102 to access the reagent container 30/sample container 40/collection container 50, etc.
Further, the housing 10 is further provided with a touch screen 103, which facilitates touch operation and display of related parameters, data and other information.
The invention also comprises 3 photoelectric switches 20 arranged on the main body frame 1, wherein the 3 photoelectric switches 20 are respectively arranged opposite to the positive pressure platform 2, the reagent platform 3 and the collection platform 5 and used for detecting whether the three reach initial positions so as to realize the automatic stop of the first driving device 61 and the second driving device 71.
Fig. 15 to 17 also show a reagent container 30, a sample container 40 and a collection container 50 of the present invention. The reagent container 30 is provided with a plurality of accommodating cavities 301 for accommodating reagents such as lysate and washing liquor, and the bottom of the reagent container 30 is provided with a silica gel plug 60 to realize movable sealing, when the reagent container 30 is connected with the sample container 40, the reagent container 30 can slowly drip liquid, and only when gas passes through, a large amount of liquid can drip into the sample container 40 under the action of air pressure, so that the experiment requirement is met; the sample container 40 is internally provided with a film which is not completely ventilated to form a semi-vacuum state; the collection container 50 is an integrated structure of a nucleic acid container 501 and a waste liquid container 502, and the nucleic acid container 501 is provided with a detachable cover 503 and can be covered by an operator after nucleic acid collection, so that the nucleic acid container 501 is sealed.
The working process of the invention is as follows:
firstly, an operator opens the flip 102 to load the reagent containers 30 containing different reagents, the sample containers 40 containing samples and the empty collecting containers 50, and then covers the flip 102;
secondly, under the action of the transverse driving assembly 6, the reagent platform 3 advances for a certain distance to enable the reagent container 30 to be aligned to the sample container 40, and then the vertical driving assembly 7 acts on the positive pressure platform 2 to enable the positive pressure platform 2 to descend to press the reagent platform 3; then the air pump assembly 8 blows air to extrude the reagent in the reagent container 30 to the sample container 40, for example, the lysis solution is extruded into the sample container 40 and waits for the reaction process;
thirdly, the process of the second step is repeated for a plurality of times to realize that different reagents are squeezed into the sample container 40 to finish the steps of cracking, precipitating, washing and the like, and finally, the nucleic acid in the sample is layered with other waste liquid;
and fourthly, under the action of the vertical driving assembly 7, the positive pressure platform 2 drives the reagent platform 3 and the membrane column platform 4 to press the collection platform 5, so that the reagent container 30, the sample container 40 and the nucleic acid container 501/waste liquid container 502 are sequentially communicated, the nucleic acid is pressed to the nucleic acid container 501, and the waste liquid is pressed to the waste liquid container 502, so that the nucleic acid extraction is completed.
Through the scheme, the air pump assembly 8 is used for supplying air to the air blowing joint 21 of the positive pressure platform 2, and the liquid in each container can be pushed to flow in an air pressure mode, so that each step of nucleic acid extraction is completed in the moving process of the positive pressure platform 2, the reagent platform 3 and the collection platform 5, and the nucleic acid extraction is realized in a layered acquisition mode after nucleic acid precipitation; the air pump assembly 8 can blow air to the plurality of air blowing connectors 21 simultaneously, so that simultaneous processing of a plurality of samples is realized in the same step, the processing efficiency of each step is ensured, and rapid and large-batch automatic extraction of nucleic acid is realized.
The above embodiments and drawings are not intended to limit the form and style of the present invention, and any suitable changes or modifications thereof by those skilled in the art should be considered as not departing from the scope of the present invention.
Claims (10)
1. A nucleic acid extraction apparatus characterized in that:
the device comprises a main body frame, and a positive pressure platform, a reagent platform, a membrane column platform, a collection platform, a transverse driving assembly, a vertical driving assembly and a pneumatic pump assembly which are arranged on the main body frame;
the positive pressure platform, the reagent platform, the membrane column platform and the collection platform are sequentially arranged from top to bottom;
the positive pressure platform vertically moves on the main body frame, and a plurality of air blowing joints are arranged on the positive pressure platform;
the reagent platform moves transversely on the main body frame and is used for bearing a reagent container;
the membrane column platform is used for bearing a sample container;
the collecting platform moves transversely on the main body frame and is used for bearing a collecting container;
the transverse driving assembly drives the reagent platform and the collection platform respectively so as to align the reagent container with the sample container and align the collection container with the sample container;
the vertical driving assembly drives the positive pressure platform to enable the reagent container, the sample container and the collection container to be connected in sequence after being aligned;
the air pump assembly is connected with the air blowing joint of the positive pressure platform and used for pushing liquid in the container to drip downwards.
2. The nucleic acid extraction apparatus according to claim 1, characterized in that:
the main body frame comprises a bottom plate, a rear plate and two side plates, wherein the rear plate and the two side plates are vertically connected to the bottom plate; the side surfaces of the two side plates are provided with a first transverse guide rail and a second transverse guide rail which are parallel to each other, the rear plate is provided with a vertical guide rail, the two ends of the reagent platform are movably matched above the two side plates and are respectively in sliding connection fit with the first transverse guide rails on the two sides, the two ends of the collecting platform are movably matched on the two side plates and are respectively in sliding connection fit with the second transverse guide rails on the two sides, and the positive pressure platform is in sliding connection fit with the vertical guide rails.
3. The nucleic acid extraction apparatus according to claim 2, characterized in that:
the transverse driving assembly comprises two groups of first driving devices, a synchronous shaft, a first synchronous belt, an idler wheel and a second synchronous belt; the first driving device and the synchronizing shaft are both arranged on the back surface of the rear plate and are in transmission connection through the first synchronizing belt, and the synchronizing shaft is parallel to the bottom plate; the idler wheel is in running fit with the side face of the side plate and is in transmission connection with the synchronous shaft through the second synchronous belt; and the reagent platform and the collection platform are respectively connected with the two groups of second synchronous belts.
4. The nucleic acid extraction apparatus according to claim 3, characterized in that:
the first driving device is a stepping motor.
5. The nucleic acid extraction apparatus according to claim 1, characterized in that:
the positive pressure platform comprises the air blowing joint, an air blowing cross rod, an air blowing transverse plate and an air blowing vertical plate; the air blowing transverse rod and the air blowing transverse plate are arranged in parallel up and down, and two ends of the air blowing transverse rod and the air blowing transverse plate are respectively and vertically connected with the upper end and the lower end of the air blowing vertical plate; the blowing cross rod is in transmission connection with the vertical driving assembly; the air blowing joints are arranged on the air blowing transverse plate along a straight line.
6. The nucleic acid extraction apparatus according to claim 1, characterized in that:
the reagent platform comprises a first placing frame, a first connecting block and a pressing strip; the first placing frame is used for bearing a reagent container and is in sliding connection and matching with the main body frame through first connecting blocks at two ends; the pressing strip is detachably matched on the first placing frame and used for fixing the reagent container.
7. The nucleic acid extraction apparatus according to claim 6, characterized in that:
the reagent platform further comprises a first bearing, a first lifting shaft and a first spring; the first bearing is arranged at the end part of the first placing frame; the first lifting shaft is movably arranged in the first bearing in a penetrating way and is vertically connected with the first connecting block; the first spring is disposed between the first bearing and the first connecting block.
8. The nucleic acid extraction apparatus according to claim 1, characterized in that:
the membrane column platform comprises a second placing frame, a limiting block, a second bearing, a second lifting shaft and a second spring; the second placing frame is used for bearing a sample container and is arranged on the main body frame through limiting blocks at two ends, and a limiting groove for embedding the limiting blocks is formed in the main body frame; a movable groove is formed in the side face of the limiting block; the second bearing is arranged at the end part of the second placing frame; the second lifting shaft movably penetrates through the second bearing and is vertically connected with two ends of the movable groove; the second spring is disposed between the second bearing and a lower end of the movable groove.
9. The nucleic acid extraction apparatus according to claim 1, characterized in that:
the air pump assembly comprises an electromagnetic valve and an air pump; the electromagnetic valve is provided with an inlet and a plurality of outlets; the output end of the air pump is communicated to the inlet of the electromagnetic valve, and the outlet of the electromagnetic valve is respectively communicated to each air blowing joint.
10. The nucleic acid extraction apparatus according to claim 9, characterized in that:
the air pump assembly further comprises a flow divider, and a pressure sensor and a flow speed regulating valve which are arranged on the flow divider; the output end of the air pump is communicated with the input end of the flow divider; the inlet of the electromagnetic valve is communicated with the output end of the shunt; the pressure sensor is used for detecting the gas pressure in the flow divider; the flow speed regulating valve is used for regulating the gas pressure in the flow divider.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210762189.2A CN115044454A (en) | 2022-06-29 | 2022-06-29 | Nucleic acid extraction apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210762189.2A CN115044454A (en) | 2022-06-29 | 2022-06-29 | Nucleic acid extraction apparatus |
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| CN115044454A true CN115044454A (en) | 2022-09-13 |
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| CN202210762189.2A Pending CN115044454A (en) | 2022-06-29 | 2022-06-29 | Nucleic acid extraction apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116875425A (en) * | 2023-07-24 | 2023-10-13 | 北京万泰生物药业股份有限公司 | Nucleic acid extraction and purification device, reagent combination, nucleic acid extraction and purification system and method |
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2022
- 2022-06-29 CN CN202210762189.2A patent/CN115044454A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116875425A (en) * | 2023-07-24 | 2023-10-13 | 北京万泰生物药业股份有限公司 | Nucleic acid extraction and purification device, reagent combination, nucleic acid extraction and purification system and method |
| CN116875425B (en) * | 2023-07-24 | 2024-03-12 | 北京万泰生物药业股份有限公司 | Nucleic acid extraction and purification device, reagent combination, nucleic acid extraction and purification system and method |
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