CN114246658A

CN114246658A - Visual double-cavity ovum taking system

Info

Publication number: CN114246658A
Application number: CN202111257980.XA
Authority: CN
Inventors: 孟荣华; 王双; 毕方成; 陈春晓; 毕胜成
Original assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Current assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2022-03-29
Anticipated expiration: 2041-10-27
Also published as: CN114246658B

Abstract

The invention discloses a visual double-cavity ovum taking system, which comprises a double-cavity ovum taking device and a negative pressure suction device; the negative pressure suction apparatus comprises a negative pressure suction apparatus body with a negative pressure interface and a negative pressure connecting pipe, wherein the negative pressure connecting pipe comprises a first adapter pipe, a second adapter pipe and a filter; the double-cavity ovum taking device comprises a negative pressure suction tube, and a puncture needle, a puncture needle holding handle and an oocyte collecting tube which are connected in sequence; one end of the oocyte collecting pipe and one end of the negative pressure suction pipe are both fixed in the sealing plug, and the sterile collecting pipe is clamped on the sealing plug to collect the oocytes; the tail end of the oocyte collecting pipe is provided with an oocyte releasing cavity; an ovum taking channel and a flushing channel are arranged in the inner cavity of the puncture needle; the sterile collection tube is placed in a thermostat for keeping the aspirated oocytes warm. The sealing plug is provided with a convex inclined plane type oocyte collecting pipe cavity, so that the ovum can fall downwards along the pipe wall, the impact force of the ovum when entering liquid is reduced, and the ovum is prevented from staying in the pipeline.

Description

Visual double-cavity ovum taking system

Technical Field

The invention relates to the technical field of egg taking surgical instruments, in particular to a visual double-cavity egg taking system.

Background

Modern people defer pregnancy plans for various reasons, so that the incidence rate of infertility tends to increase year by year, the advanced age is one of the reasons for the occurrence of the infertility, in addition, the infertility is also influenced by factors such as environment, self pressure, drug abuse and the like, the incidence rate also tends to increase year by year, and more infertility patients are treated by assisted reproduction technology, particularly in vitro fertilization. Currently, human ART mainly includes intrauterine fertilization, in vitro fertilization-embryo transfer, artificial insemination, in vitro maturation of ova, injection of single sperms of oocyte plasma, freezing and thawing of ova, sperm and embryo, genetic diagnosis before transfer, and the like. The treatment aim of a patient is to obtain clinical pregnancy and healthy offspring, and the aim is to obtain high-quality ova, improve the fertility rate and finally obtain more high-quality embryos on the premise of achieving the aim, but the quality of the ova and the embryos can be influenced in a plurality of links such as clinical medication, an ovum taking operation, embryo culture-transplantation and the like. In vitro fertilization-embryo transfer (IVF-ET) cycle usually performs artificial ovarian hyperstimulation, which aims to stimulate a plurality of primordial follicles to develop synchronously to obtain more high-quality ova so as to obtain high-quality embryos for selection and transfer, and finally improve the clinical pregnancy rate.

The method comprises the following steps of collecting mature oocytes from an ovarian tissue through a vagina under the guidance of B ultrasonic, wherein the mature oocytes are an intermediate link from a human body to a laboratory incubator for culture, the oocytes and follicular fluid are firstly sucked out from an ovary, and then the sucked-out materials are stored in a test tube.

In addition, as shown in fig. 1, the conventional ovum fetching device generally comprises a puncture needle, a collecting tube 5 ', a negative pressure suction tube 6' and a sealing plug 7 ', wherein the ovum directly enters the test tube through the collecting tube 5', which causes inevitable damage in the ovum collecting process, and in addition, after most of the oocyte is sucked out, the residual oocyte attached to the ovarian tissue is difficult to suck out, which affects the oocyte collecting rate. Therefore, how to reduce the impact of the ovum in the collection process becomes a problem which needs to be solved at present.

Disclosure of Invention

The invention aims to provide a visual double-cavity ovum taking system, which enables an ovum to descend downwards along the wall of a test tube in an ovum taking operation under the action of negative pressure, reduces the impact force of the ovum when descending into liquid and reduces the deformation damage of the ovum.

In order to achieve the aim, the invention provides a visual double-cavity ovum taking system which comprises a double-cavity ovum taking device and a negative pressure suction device which are communicated through a negative pressure connecting pipe; the negative pressure aspirator includes: a negative pressure aspirator body having a negative pressure interface; the negative pressure connecting pipe is provided with a first adapter pipe and is connected with the negative pressure interface through the first adapter pipe; the first adapter tube is connected with the second adapter tube through a filter; the double-cavity ovum taking device comprises a negative pressure suction tube, and a puncture needle, a puncture needle holding handle and an oocyte collecting tube which are connected in sequence; the end parts of the oocyte collecting pipe and the negative pressure suction pipe are embedded in the sealing plug, and the sterile collecting pipe is clamped on the sealing plug and is used for collecting the sucked oocytes; an oocyte release cavity is formed in the sealing plug and at the tail end of the oocyte collecting pipe, and the oocyte release cavity is communicated with the cavity of the oocyte collecting pipe; the puncture needle inner cavity is provided with an ovum taking channel and a flushing channel in parallel along the length direction. The sterile collection pipe is placed in the thermostat and used for preserving heat of the oocytes sucked from the sterile collection pipe; the thermostat comprises a thermostat housing comprising a floor and a chamber disposed at a top of the floor; the chamber is of a closed structure and comprises at least two observation windows; the thermostat is internally provided with a heating mechanism and a plurality of test tube grooves, the test tube grooves are used for placing test tubes, and the heating mechanism is a plurality of the test tube grooves provide a constant temperature environment.

According to the invention, relative to the small-diameter bottom plane of the sealing plug, the oocyte collection tube cavity is of a convex structure and extends towards the interior of the sterile collection tube; an inclined surface structure is formed on the part of the oocyte collecting pipe cavity, which faces outwards and is close to the pipe wall of the sterile collecting pipe, so that the ovum is protected from falling downwards along the pipe wall of the sterile collecting pipe connected with the oocyte collecting pipe cavity.

According to the invention, the inclination of the inclined plane structure is 20-45 degrees by taking the axial direction of the sealing plug as a reference. Preferably, the inclination of the inclined surface structure is 30-40 °, for example 15 °, 25 °, 35 °, 45 °, 55 ° or any point value in a range of values consisting of any two points.

According to the invention, the joint of the oocyte collecting tube cavity and the oocyte release cavity is in a concave structure. Preferably, a negative pressure tube cavity is formed at the end part of the negative pressure suction tube positioned in the sealing plug, and the tail end of the negative pressure suction tube is also connected with a negative pressure suction tube connecting seat which is used for being communicated with a negative pressure device; and relative to the small-diameter bottom plane of the sealing plug, the cavity of the negative pressure pipe extends to the pipe orifice of the sterile collecting pipe and is of a concave structure.

According to the invention, the joint of the oocyte release cavity and the oocyte collecting pipe cavity is also provided with an injector connecting port, the injector connecting port is connected with an injection pipe, and the ovum taking pipeline is cleaned by injecting liquid into the oocyte collecting pipe and the puncture needle before taking the ovum. Preferably, the tube orifice of the sterile collection tube is clamped at the upward third position of the bottom of the sealing plug.

According to the invention, a puncture needle handle of the double-cavity ovum taking device is communicated with a flushing pipe and a flushing pipe connecting seat positioned at one end of the flushing pipe; the flushing pipe connecting seat is communicated with a constant-temperature push injection pump and is used for flushing the follicle.

According to the invention, the puncture needle protection sleeve and the oocyte collection tube protection sleeve are also included; the puncture needle protective sleeve is used for protecting the puncture needle in the egg taking and packaging process; the oocyte collecting tube protective sleeve sets up in the oocyte collecting tube with the junction of handle is held to the pjncture needle to avoid buckling and causing the oocyte collecting tube blocks up, leads to the oocyte to block or lose.

Preferably, the puncture needle handle is in a cylindrical shape with a thin middle part and thick two ends; the oocyte collecting pipeline consists of a plurality of layers of sleeves, and the plurality of layers of sleeves are made of materials with heat exchange media. Preferably, the volume of the flushing channel in the puncture needle cavity is about one eighth to one tenth of the volume of the egg taking channel.

According to the invention, the filter is screwed to the first adapter tube and the second adapter tube; the filter comprises a filter shell and a filter membrane, wherein the diameter of the filter membrane is

The pore diameter was 0.22. mu.m.

According to the invention, the filter shell is made of polypropylene, and the filter membrane is a polytetrafluoroethylene microporous membrane.

According to the invention, the first adapter tube and the second adapter tube have an inner diameter of 6.5mm and an outer diameter of 11 mm.

According to the invention, the negative pressure range of the negative pressure aspirator is-10 to-500 mmHg.

According to the invention, the negative pressure aspirator body further comprises a display screen, a shell and a sealing ring for sealing the joint of the display screen and the shell. The display screen is provided with a negative pressure adjusting button, and the negative pressure adjusting button comprises a rising negative pressure adjusting key, a falling negative pressure adjusting key and a negative pressure direct rising key for increasing the negative pressure instantly.

According to the invention, the heights of the two sides of the foot switch are higher than the height of the middle foot position, so that a doctor can be prevented from stepping on the foot switch by mistake. Preferably, the foot pedal comprises a self-locking foot pedal and a non-self-locking foot pedal mode.

According to the invention, the test tube slot is arranged in the chamber, and an opening for inserting the test tube is arranged at the position of the chamber corresponding to the top of the test tube slot. Preferably, the heating mechanism comprises a heat-generating metal disposed within the base plate. More preferably, the heat-generating metal comprises a wire, a metal tube or a metal block. Most preferably, the heat-generating metal comprises copper, aluminum, iron, e.g. aluminum blocks.

According to the invention, the heating mechanism comprises a heating layer arranged outside the test tube groove. Preferably, a heat insulation layer is arranged between the heating layer and the test tube groove. Preferably, the heating layer is coated at the middle lower part of the test tube groove. More preferably, the heat generating layer covers at least 1/5 test tube slots.

According to the invention, the heating layer comprises conductive adhesive and carbon fibers, the carbon fibers are distributed in the conductive adhesive, and the conductive adhesive is connected with an external power supply through a conductive wire and/or conductive cloth. Preferably, the heat insulation layer is detachably connected to the bottom plate. More preferably, the bottom heating mechanism is sleeved on a heat insulation layer, and the heat insulation layer is preferably made of ceramic.

According to the invention, the heating mechanism comprises a plurality of wind-heat pipes arranged in the cavity, the air inlet ends of the wind-heat pipes penetrate through the bottom plate to be connected with an external hot air generator, and the air outlet ends of the wind-heat pipes are fixed on the inner walls of the observation windows or the side plates. More preferably, the air outlet ends of the plurality of air heat pipes are inclined towards the center of the chamber, the inclination angle is 20-35 degrees, and hot air of the plurality of air heat pipes forms a rotational flow in the chamber under the inclination angle, so that the temperature of each corner in the chamber is consistent.

According to the invention, temperature sensors are arranged in the cavity and on the outer wall of the test tube groove. Preferably, the temperature sensor is connected with a temperature controller, the temperature controller is used for controlling the temperature of the heat generating mechanism, and the temperature controller can be arranged on the shell or independently arranged outside the shell. Preferably, an operation interface is further arranged on the display, and the temperature controller is arranged on the operation interface.

According to the invention, the cross section of the chamber is circular, a regular polygon with sides greater than or equal to 4, or an anisotropic structure. Preferably, the cross-section of the chamber is rectangular, hexagonal or octagonal, for example rectangular. Preferably, the chamber is provided with at least one observation window corresponding to each test tube slot, for example, two observation windows corresponding to each test tube slot.

More preferably, the chamber comprises a plurality of side plates connected in sequence, and the observation window is arranged on the side plates. More preferably, the side plate is a transparent structure, that is, the side plate is a viewing window.

Further preferably, the top of the chamber is further provided with a top plate, and an opening is formed in the top plate corresponding to the top of the test tube slot and used for inserting the test tube into the test tube slot. Most preferably, the top plate, the side plates and the bottom plate form a closed chamber, and the top plate and the side plates are made of metal, for example, aluminum, and are integrally formed.

The invention has the beneficial effects that:

1) in the prior art, the ovum directly enters the test tube through the collecting tube, so that the damage to the ovum is large, but the collecting tube cavity with a convex structure is arranged on the sealing plug of the invention, and the ovum enters the test tube through the collecting tube cavity, so that the ovum can fall downwards along the wall of the test tube, the impact force of the ovum when falling into liquid is reduced, the deformation damage of the ovum is reduced, and the ovum is protected and collected. In addition, the ovum taking device sealing plug is also provided with a negative pressure tube cavity with a concave structure, so that the backflow of liquid in the negative pressure suction process can be prevented, and the device is protected; while allowing each tube to be filled with a greater volume of liquid. The sealing plug is also provided with an ovum release cavity structure for releasing and buffering the ovum, so that the ovum forms a larger droplet ball, the ovum is protected in the falling process, and the ovum is prevented from staying in a pipeline.

2) The invention designs a double-cavity ovum taking device aiming at the situation that the collection rate of the oocytes is influenced because the ovum taking device sucks most of the oocytes and the residual oocytes adhered to the ovarian tissue are difficult to suck out.

3) According to the negative pressure aspirator structure, the filter is arranged in the disposable negative pressure connecting pipe between the negative pressure pump and the egg taking device, and the filter has a bidirectional protection function, so that on one hand, the phenomenon that liquid enters the negative pressure aspirator due to overlarge suction amount is prevented, and the equipment is damaged and loses efficacy due to the fact that the liquid enters the negative pressure aspirator is avoided. The existing negative pressure suction device is not provided with a filter, liquid suck-back and pollution risks are easily caused, and the structural design of the negative pressure suction device can prevent the liquid suck-back and avoid the pollution risks. On the other hand, the negative pressure pump belongs to a non-sterile product, the disposable negative pressure connecting pipe belongs to a sterile product, and when the negative pressure pump stops working and releases vacuum, vacuum gas released by the pump can be filtered, so that the gas released by the pump is prevented from polluting liquid in a test tube.

4) The thermostat that adopts at present usually is provided with the observation window including the casing that has accommodation space, one side of casing, heating backboard, opposite side including having, and the observation window passes through the magnetic stripe and is connected with the casing. When the disinfection device is used, due to the fact that a gap exists at the joint of the observation window and the shell, disinfection liquid easily enters the shell in the disinfection process, a heating circuit is short-circuited, and danger is caused. The thermostat comprises at least two observation windows, so that medical staff can observe the state of liquid in the test tube conveniently, the shell comprises a closed cavity and a bottom plate, when the thermostat is used, the cavity is in a closed state, disinfectant in the disinfection process can be prevented from entering the shell to generate short circuit, and the thermostat is safe.

5) The heating mechanism comprises heating metal arranged on the bottom plate, the cavity is of a closed structure formed by integrally molding metal materials, and compared with the prior art that heating is carried out through the heating plates arranged on the side edges, the heat transmission speed is low, and the temperatures of all parts are uneven, the heat in the heating mechanism can be quickly transmitted, so that the temperatures of all parts in the cavity are consistent. The end of giving vent to anger of wind heat pipe inclines to the cavity center, and inclination is 20-35, and under this inclination, the hot-blast whirl that forms of a plurality of wind heat pipes in the cavity for the temperature at each corner is unanimous in the cavity, and the wind heat pipe utilizes preheating of high temperature sterilization to heat the cavity, can effectively reduce cost.

6) The bottom heating mechanism comprises conductive adhesive and carbon fibers, the carbon fibers are distributed inside the conductive adhesive, the conductive adhesive is connected with an external power supply through a conductive wire and/or conductive cloth, the bottom heating mechanism is sleeved on the heat insulation layer, heat transfer is fast, temperature change caused by heat dissipation can be avoided, and constant temperature is effectively maintained.

Drawings

FIG. 1 is a schematic view of a partial sealing plug of a prior art ovipositor;

fig. 2 is a schematic diagram of the overall structure of the visual double-cavity egg taking system.

Fig. 3 is a schematic structural diagram of the visual double-cavity egg taking system.

Fig. 4 is a schematic structural diagram of a sealing plug of the visual double-cavity ovum taking system.

Fig. 5 is an enlarged structural schematic diagram of the section A-A of the puncture needle of the visual double-cavity ovum taking system.

Fig. 6a is a schematic view of the overall appearance structure of the vacuum extractor of the present invention.

FIG. 6b is a schematic view of the external structure of the display screen of the vacuum extractor of the present invention.

Fig. 7 is a schematic structural view of a disposable negative pressure connecting tube of the negative pressure suction apparatus of the present invention.

FIG. 8 is a schematic diagram of the construction of a thermostat in the visual double-lumen egg retrieval system of the present invention;

FIG. 9 is a schematic view showing an arrangement structure of the heating plate and the test tube well.

In the figure: 1-puncture needle protective sleeve; 2, puncturing needle; 3-puncture needle handle holding; 4-connecting a protective sleeve; 5-a collecting pipe; 6-negative pressure suction tube; 7-a sealing plug; 8-negative pressure suction connecting seat; 9-ovum release chamber; 10-negative pressure tube cavity; 11-collection tube cavity; 12-syringe connection port; 13-a flushing pipe; 14-a flushing pipe connecting seat; 110-inclined surface structure, 20-ovum taking channel (main channel), 21-needle core, 22-needle tube and 23-flushing channel (auxiliary channel); 100-negative pressure aspirator body; 200-a display screen; 300-a housing; 400-sealing ring; 500-negative pressure interface; 600-foot pedal; 700-a transfer tube; 800-a filter; 900-connecting pipe; 1001-negative pressure connecting pipe; 01-a thermostat housing; 02-a bottom plate; 03-a chamber; 04-a heating mechanism; 05-a test tube groove; 06-side plate; 07-a temperature controller; 08-a display; 09-a top plate; 010-opening.

Detailed Description

The following detailed description of embodiments of the invention, but the invention can be practiced in many different ways, as defined and covered by the claims.

When the ovum fetching device sucks most of the oocytes, the residual oocytes attached to the ovarian tissues are difficult to suck, and the collection rate of the oocytes is influenced, and the invention designs the double-cavity ovum fetching device aiming at the condition, wherein the double cavities refer to that one cavity is an ovum fetching channel 20 and the other cavity is a flushing channel 23.

As shown in fig. 2 to 9, the present invention provides a visual double-chamber ovum taking system, which comprises a double-chamber ovum taking device and a negative pressure suction device, wherein the double-chamber ovum taking device and the negative pressure suction device are communicated through a negative pressure connecting tube 1001. The negative pressure suction apparatus comprises a negative pressure suction apparatus body 100 and a negative pressure connecting pipe 1001, wherein the negative pressure suction apparatus body 100 is provided with a negative pressure interface 500, the negative pressure connecting pipe 1001 is provided with a first adapter pipe 700 and is connected with the negative pressure interface 500 through the first adapter pipe 700, and the first adapter pipe 700 is connected with a second adapter pipe 900 through a filter 800. The double-cavity ovum taking device comprises a negative pressure suction tube 6, and a puncture needle 2, a puncture needle handle 3 and an oocyte collecting tube 5 which are connected in sequence. The sealing plug 7 is arranged at the end parts of the oocyte collecting pipe 5 and the negative pressure suction pipe 6 and fixed, the oocyte collecting pipe 5 is embedded inside the sealing plug 7, and the sterile collecting pipe is clamped on the sealing plug 7 and used for collecting sucked oocytes. Inside the sealing plug 7, the tail end of the oocyte collecting tube 5 is provided with an ovum release cavity 9, and the ovum release cavity 9 is communicated with a collecting tube cavity 11.

Wherein, an ovum taking channel 20 (main channel) and a flushing channel 23 (auxiliary channel) are arranged in parallel along the length direction in the cavity of the puncture needle 2. The puncture needle handle 3 of the double-cavity ovum picker is communicated with a flushing pipe 13 and a flushing pipe connecting seat 14 positioned at one end of the flushing pipe 13, the flushing pipe connecting seat 14 is communicated with a follicle flushing injection device, and after most of oocytes are sucked out, a flushing fluid can be injected into the ovary through a flushing channel 23 of the puncture needle to flush the residual oocytes so as to suck out the residual oocytes smoothly.

In the prior art, the oocyte directly enters the sterile collection tube through the oocyte collection tube, and the damage to the ovum is large, but the oocyte collection tube cavity 11 with the bulge structure is arranged in the sealing plug 7, the bulge structure is provided with the bevel opening design facing to the outer part, so that the oocyte can fall downwards along the tube wall of the sterile collection tube, the impact force when the oocyte falls into liquid is reduced, the deformation damage of the oocyte is reduced, and the oocyte is protected. The invention is also provided with an oocyte release cavity 9 which is used for releasing and buffering the ovum, so that the ovum forms a larger dropping ball, the ovum is protected in the falling process, and the ovum is prevented from staying in the pipeline. Preferably, the junction of the oocyte collection tube cavity 11 and the oocyte release chamber 9 is in a recessed U-shaped structure.

In addition, the sealing plug 7 is also provided with a negative pressure pipe cavity with a concave structure, so that liquid can be prevented from flowing backwards in the negative pressure suction process, and equipment is effectively protected; meanwhile, the negative pressure tube cavity with the concave structure reduces the occupied volume in the sterile collection tube, so that each sterile collection tube is filled with more liquid.

In a preferred embodiment of the invention, as shown in figure 4, the oocyte collection tube cavity 11 is in a convex configuration relative to the bottom plane of the sealing plug 7 (the end of the sealing plug with the smaller diameter) and extends into the interior of the sterile collection tube. The inclined surface structure 110 is formed on the part of the oocyte collecting pipe cavity 11, which faces to the outer side and is close to the pipe wall of the sterile collecting pipe, so that the ovum is protected from falling downwards along the pipe wall of the sterile collecting pipe connected with the oocyte collecting pipe, the impact force of the ovum when the ovum falls into liquid is reduced, and the ovum is prevented from being deformed and damaged. Meanwhile, the collecting pipe cavity 11 can be used as a collecting pipe for collecting the ovum.

Preferably, the inclination of the inclined surface structure 110 is 20-45 ° with respect to the axial direction of the sealing plug 7, more preferably 30-40 °, such as 15 °, 25 °, 35 °, 45 °, 55 ° or any point in a range of values consisting of any two points.

According to the invention, a negative pressure tube cavity 10 with a larger space is formed at the end part of the negative pressure suction tube 6 positioned in the sealing plug 7, the tail end of the negative pressure suction tube 6 is connected with a negative pressure suction tube connecting seat 8 for communicating with a negative pressure absorber, the other end of the negative pressure suction tube is connected with the sealing plug 7, and the negative pressure suction tube connecting seat 8 is connected with the negative pressure suction tube 6 and a negative pressure suction device.

As shown in fig. 4, the negative pressure tube cavity 10 extends toward the tube opening of the sterile collection tube and is concave with respect to the small diameter bottom plane of the sealing plug 7. That is, the bottom end of the oocyte collection tube cavity 11 is below the level of the bottom surface of the sealing plug 7, while the negative pressure tube cavity 10 is above the level of the bottom surface of the sealing plug 7. The invention arranges a negative pressure tube cavity 10 at the end of a negative pressure suction tube 6, which aims to prevent liquid from flowing backwards in the negative pressure suction process; negative pressure pipe cavity 10 is the concave yield formula design for sealing plug 7, has increased aseptic collection test tube space, can make every aseptic collection test tube fill the washing liquid volume more. Preferably, the mouth of the sterile collection tube is snapped into place at the bottom third of the sealing plug 7.

As shown in figure 3, the double-cavity ovum taking device comprises a puncture needle protective sleeve 1 and an oocyte collecting pipe protective sleeve 4. The puncture needle protective sleeve 1 is used for protecting the puncture needle 2 in the egg taking and packaging process, and avoids packaging damage and product failure caused by exposure of the puncture needle 2. The oocyte collecting pipe protective sheath 4 sets up in the junction of oocyte collecting pipe 5 and pjncture needle handle 3 to avoid buckling and cause the jam of oocyte collecting pipe 5, lead to the ovum to block or lose. The puncture needle handle 3 is in a cylindrical shape with a thin middle part and thick two ends, and the shape is beneficial for a doctor to hold the ovum taking needle, so that the doctor can conveniently operate in an operation.

Preferably, the oocyte collecting tube 5 is composed of a plurality of layers of sleeves, and the plurality of layers of sleeves are made of materials with heat exchange media, so that the oocyte collecting tube 5 has a heat preservation function. More preferably, the volume of the flushing channel 23 in the cavity of the puncture needle 2 is about one eighth to one tenth of the volume of the ovum taking channel 20.

As shown in figure 4, a syringe connecting port 12 is also arranged at the joint of the oocyte release cavity 9 and the oocyte collecting tube cavity 11, the syringe connecting port 12 is connected with an injection tube, and cleaning solution is injected into the oocyte collecting tube 5 and the puncture needle 2 before egg taking for cleaning the oviduct.

As shown in fig. 5, the puncture needle 2 is a double-lumen puncture needle, i.e., a main channel (an ovum-taking channel 20) for taking out an oocyte is formed in the needle core 21, and an auxiliary channel (a flushing channel 23) is formed between the needle core 21 and the needle cannula 22 for injecting a flushing liquid to flush the residual oocyte. One end of the flushing pipe 13 is communicated with the flushing channel 23 through the puncture needle handle 3, the other end is connected with a flushing pipe connecting seat 14, and the oocyte collecting pipeline 5 is communicated with the ovum taking channel 20 through the puncture needle handle 3. Preferably, an ultrasonic echo zone with the length of 6-9mm is also arranged at the head end of the puncture needle 2.

As shown in fig. 6 and 7, the vacuum extractor of the present invention includes a vacuum extractor body 100, and a vacuum connection pipe 1001, a foot switch, and a power line connected thereto. The vacuum extractor body 100 is provided with a display screen 200, a casing 300 and a sealing ring 400 for sealing the joint of the display screen 200 and the casing 300. The negative pressure suction apparatus body 100 is provided with a negative pressure pump for providing negative pressure. The display screen 200 has a display and operation interface, can display negative pressure and set negative pressure, the shell 300 plays a role in fixing and supporting, and the sealing ring 400 can enhance the sealing performance of the product.

The display screen 200 is provided with a negative pressure interface 500, the negative pressure connection pipe 1001 includes a first adapter pipe 700 and a second adapter pipe 900, the first adapter pipe 700 is connected with the second adapter pipe 900 through a filter 800, and preferably, a threaded connection is adopted. The first adapter tube 700 is connected to the negative pressure port 500, and the second adapter tube 900 is connected to the filter 800 and the ovum pickup device. The filter 800 prevents liquid from being sucked back into the negative pressure pump while having a filtering effect on gas. Preferably, the first adapter tube 7000 has an inner diameter of 6.5mm and an outer diameter of 11 mm.

According to the invention, filter 800 comprises a filter housing and a filter membrane having a diameter of

The pore diameter was 0.22. mu.m. Wherein, the filter shell is made of polypropylene material, and the filter membrane is a polytetrafluoroethylene microporous membrane. The polytetrafluoroethylene microporous membrane (PTFE) has unique node fibrillating property, smooth surface, chemical substance resistance, air permeability and water impermeability.

As shown in fig. 6b, the display screen 200 is provided with a negative pressure adjusting button, which includes a rising negative pressure adjusting key, a falling negative pressure adjusting key, and a negative pressure raising key for instantly increasing the negative pressure.

The negative pressure connection pipe 1001 is used to provide a negative pressure gas passage, and may be a disposable negative pressure connection pipe. The disposable negative pressure connecting pipe is adopted, and the using principle that one person disposes one pipe at a time is executed, so that the pollution source is stopped, and the safety of the ovum taking operation is ensured.

The foot switch is connected with the negative pressure aspirator body 100 through an electric wire and is used for controlling the state of the negative pressure pump. The negative pressure of the negative pressure aspirator can be set to two modes of mmHg and kPa, and the negative pressure range is between-10 mmHg to-500 mmHg or-1.0 kPa to-67.0 kPa. Preferably, the foot switch comprises two modes of a self-locking foot switch and a non-self-locking foot switch, wherein the non-self-locking foot switch needs to continuously step on the foot switch, the foot switch is loosened, negative pressure is not provided any more, and suction is stopped; in the self-locking pedal mode, the pedal switch is needed to be stepped once to start working and stepped once again, negative pressure is not provided any more, and suction is stopped. Preferably, the heights of the two sides of the foot switch are higher than the height of the middle foot position, so that the doctor can be prevented from stepping on the foot switch by mistake.

The negative pressure suction apparatus is suitable for sucking body fluid and cells, the negative pressure suction apparatus body is connected with a disposable negative pressure connecting pipe, the disposable negative pressure connecting pipe is connected with an ovum taking device, the ovum taking device is connected on a test tube in a test tube heater, the ovum taking device is inserted into a follicle under the ultrasonic guidance, a foot switch of the negative pressure suction apparatus is stepped to suck follicular fluid, the oocyte and the follicular fluid enter a collection container test tube, and the foot switch is released after the follicle is emptied. If the aspiration is not moved in the egg taking negative pressure aspiration process and blood clots are blocked, the negative pressure rising key can be clicked to instantly increase the negative pressure and break the blockage.

According to the invention, the control switch can be arranged at the rear side of the foot switch, the foot switch can control the negative pressure aspirator only by stepping on the foot, and when the operation is stable, the control switch can be turned on by stepping on the foot, so that the negative pressure aspirator is always in an open state, the feet of workers can be liberated, and the ovum taking operation of the workers is more convenient.

As shown in FIGS. 8 and 9, the sterile collection tube is placed in a thermostat for incubating the aspirated oocytes in the sterile collection tube. The thermostat comprises a thermostat housing 01, the thermostat housing 01 comprising a base plate 02 and a chamber 03 arranged on top of the base plate 02. The chamber 03 is a closed structure and includes at least two viewing windows. Be provided with heating mechanism 04 in the thermostat and be used for placing a plurality of test tube groove 05 of test tube, heating mechanism 04 provides the constant temperature environment for test tube groove 05.

In the present invention, the test tube slot 05 is disposed in the chamber 03, and an opening for inserting the test tube is disposed at a position of the chamber 03 corresponding to the top of the test tube slot 05. Preferably, the heating mechanism 04 comprises a heat-generating metal disposed within the base plate 02. The heat generating metal is preferably a wire, a metal tube or a metal block, for example the heat generating metal may be copper, aluminum or iron.

In a preferred embodiment of the present invention, the heating mechanism 04 comprises a heat-generating layer disposed outside the test tube slot 05, and a heat-insulating layer is disposed between the heat-generating layer and the test tube slot 05. Preferably, the heat generating layer is coated on the middle lower part of the test tube slot 05. More preferably, the heat-generating layer covers at least 1/5 of the outer surface of the test tube slot 05.

According to the invention, the heating layer comprises conductive adhesive and carbon fibers, the carbon fibers are distributed in the conductive adhesive, and the conductive adhesive is connected with an external power supply through a conductive wire and/or conductive cloth. Preferably, the heat insulating layer is detachably connected to the bottom plate 02. More preferably, the heating mechanism 04 at the bottom is sleeved on a heat insulation layer, and the heat insulation layer is preferably made of ceramic.

In a preferred embodiment of the present invention, the heating mechanism 04 comprises a plurality of wind heat pipes disposed in the chamber 03, air inlet ends of the wind heat pipes penetrate through the bottom plate 02 to be connected with an external hot air generator, and air outlet ends of the wind heat pipes are fixed on the inner wall of the observation window or the side plate 06.

In a specific embodiment of the invention, the air outlet ends of the plurality of wind heat pipes are inclined towards the center of the chamber, and the inclination angle is 20-35 degrees. Preferably, temperature sensors are arranged in the chamber 03 and on the outer wall of the test tube groove. The temperature sensor is connected to the temperature controller 07 to control the temperature of the heating mechanism 04. The temperature controller 07 may be disposed on the housing or disposed outside the housing.

Wherein, the cross section of the cavity 03 is a regular polygon with a circular side edge being more than or equal to 4 or an anisotropic structure. The cross-section of the chamber 03 is preferably rectangular, hexagonal or octagonal, and may for example be rectangular.

Preferably, at least one observation window is arranged in the chamber 03 corresponding to each test tube slot 05, for example, two observation windows can be arranged in each test tube slot. The cavity 03 comprises a plurality of side plates 06 connected in sequence, and the observation window is arranged on the side plates 06. In one embodiment of the present invention, the side panel 06 is a transparent structure, that is, the side panel 06 is a viewing window.

In an embodiment of the present invention, a heating mechanism and 2 rows of 12 test tube slots are disposed in the thermostat, the test tube slots are used for placing test tubes, the heating mechanism 04 provides a constant temperature environment for the test tube slots, and two observation windows are disposed in the chamber corresponding to each test tube slot 05. The chamber 03 comprises four side plates 06 connected in sequence, the observation window is arranged on the side plates 06 and fixed with the side plates 06 through a magnetic tape or embedded inside the side plates 06. The top of the chamber is provided with a top plate 09, an opening 010 is arranged at the top of the top plate 09 and the top of the test tube groove 05, and is used for inserting a test tube into the test tube groove 05, and the side plate 06, the top plate 09 and the bottom plate 02 are formed by integrally molding aluminum materials. Heating mechanism 04 is including setting up the aluminium pig 05 in the bottom plate, and inside the cavity 03, test tube groove 05 outer wall all are provided with the temperature-sensing ware, and this temperature-sensing ware is connected with temperature controller 07, is provided with temperature controller 07 and display 08 on the thermostat casing 01, and display 08 is used for showing the temperature that temperature-sensing ware induced and the temperature that temperature controller 07 set for.

The visual double-cavity ovum taking system is mainly used for collecting ova by puncturing follicles under the guidance of abdominal or vaginal ultrasound, and the specific use method is as follows:

1) firstly, the sealing plug is connected with the sterile collecting pipe, the negative pressure aspirator is connected with a negative pressure absorber connecting seat 8 at the tail end of the negative pressure connecting pipe, and the sterile collecting pipe is connected with the sealing plug 7.

2) The puncture needle part is fixed on a B-ultrasonic vaginal probe and then is arranged in a female vagina (the front end of the puncture needle 2 is provided with an ultrasonic developing device, so that the position of the puncture needle 2 can be observed under the guidance of vaginal ultrasound), at the moment, images of a honeycomb ovary and an ultrasonic echo area at the end part of the puncture needle 1 can be seen on a B-ultrasonic display screen, a doctor can operate the layer puncture needle 2 on the probe according to the images, and the puncture needle 2 punctures into a follicle through the vagina under the guidance of the vaginal ultrasound. When the puncture needle 2 reaches the surface of the follicle, a proper force is applied after stopping slightly, so that the puncture needle 2 quickly penetrates into the follicle cavity.

3) And confirming that the needle tip is positioned in the follicle cavity, starting the negative pressure aspirator, and sequentially sucking each follicle fluid by using the negative pressure formed by the negative pressure aspirator to obtain the ovum in the follicle until the follicle collapses. After the follicular fluid of one follicle is exhausted, the puncture needle is inserted into the adjacent larger follicle. If the number of dominant follicles is less than 4-5 or more immature follicles exist, a double-cavity ovum-taking needle is applied, and the follicle cavity can be washed by culture solution for multiple times and repeatedly pumped for 2-3 times if necessary; if the number of dominant follicles exceeds 4-5, a single-cavity ovum picker (an ovum picker without a flushing device) can be used, if the number of the ova picked up is found to be less than that of the ova picked up under ultrasound in the ovum picking process, a double-cavity ovum picker is adopted, and each follicle is flushed with a culture solution (namely flushing solution) for 2-3 times so as to obtain more oocytes and avoid the loss phenomenon. The sucked follicular fluid enters the oocyte collecting pipeline through the oviduct of the puncture needle inner cavity and finally reaches the sterile collecting test tube.

4) When the residual oocytes attached to the ovarian tissue are difficult to suck out, the syringe can be used for injecting the flushing device to inject flushing fluid into the flushing channel of the puncture needle and reach the ovary, the pumping process is repeated, and the residual oocytes are continuously pumped out. After the ovum is taken out, the puncture needle 2 is withdrawn to the outside of the body. The pelvic cavity is scanned conventionally to check whether internal bleeding or hematoma is formed.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A visual double-cavity ovum taking system is characterized by comprising a double-cavity ovum taking device and a negative pressure suction device which are communicated through a negative pressure connecting pipe (1001); the negative pressure aspirator includes:

a negative pressure aspirator body (100), the negative pressure aspirator body (100) having a negative pressure interface (500);

a negative pressure connection pipe (1001) having a first adapter pipe (700) and connected to the negative pressure connection (500) via the first adapter pipe (700); the first adapter tube (700) is connected with the second adapter tube (900) through a filter (800);

the double-cavity ovum fetching device comprises: a negative pressure suction tube (6), and a puncture needle (2), a puncture needle handle (3) and an oocyte collecting tube (5) which are connected in sequence; the end parts of the oocyte collecting pipe (5) and the negative pressure suction pipe (6) are embedded in a sealing plug (7), and the pipe orifice of the sterile collecting pipe is clamped on the sealing plug (7) and used for collecting the sucked oocytes; an oocyte release cavity (9) is arranged at the tail end of the oocyte collecting pipe (5) in the sealing plug (7), and the oocyte release cavity (9) is communicated with an oocyte collecting pipe cavity (11); an ovum taking channel (20) and a flushing channel (23) are arranged in parallel in the inner cavity of the puncture needle (2) along the length direction;

the sterile collection pipe is placed in the thermostat and used for preserving heat of the oocytes sucked from the sterile collection pipe; the thermostat comprises a thermostat housing (01), wherein the thermostat housing (01) comprises a bottom plate (02) and a chamber (03), and the chamber (03) is arranged on the top of the bottom plate (02); the chamber (03) is of a closed structure and comprises at least two observation windows; the thermostat is provided with a heating mechanism (04) and a plurality of test tube grooves (05), the test tube grooves (05) are used for placing test tubes, and the heating mechanism (04) is used for providing a constant temperature environment for the test tube grooves (05).

2. A visual double-lumen aspiration system according to claim 1, wherein the oocyte collection tube cavity (11) is in a bulge configuration relative to the minor diameter bottom plane of the sealing plug (7) and extends inwardly of a sterile collection tube; the part of the oocyte collecting pipe cavity (11) facing to the outer side and close to the pipe wall of the sterile collecting pipe is provided with an inclined surface structure (110) so as to protect the ovum from falling downwards along the pipe wall of the sterile collecting pipe connected with the oocyte collecting pipe cavity.

3. The visual double-lumen egg aspiration system as claimed in claim 2, wherein the inclination of the inclined surface structure (110) is 20-45 ° with respect to the sealing plug (7) axial direction.

Preferably, the inclination of the inclined surface structure (110) is 30-40 °, for example 15 °, 25 °, 35 °, 45 °, 55 ° or any point value in a range of values consisting of any two points.

4. The visual double-cavity egg taking system as claimed in claim 2, wherein the joint of the oocyte collection tube cavity (11) and the oocyte release cavity (9) is in a concave structure.

Preferably, a negative pressure tube cavity (10) is formed at one end part of the negative pressure suction tube (6) located in the sealing plug (7), and the other end of the negative pressure suction tube (6) is connected with a negative pressure suction tube connecting seat (8) for communicating with a negative pressure device.

5. The visual double-cavity egg taking system according to claim 1, wherein a syringe connecting port (12) is further formed at the joint of the oocyte release cavity (9) and the oocyte collecting pipe cavity (11), and the syringe connecting port (12) is connected with a syringe and is used for injecting liquid into the oocyte collecting pipe (5) and the puncture needle (2) to clean an egg taking duct before taking eggs.

6. The visual double-cavity ovum taking system as claimed in claim 1, wherein a puncture needle handle (3) of the double-cavity ovum taking device is communicated with a flushing pipe (13) and a flushing pipe connecting seat (14) which is positioned at one end of the flushing pipe (13); the flushing pipe connecting seat (14) is communicated with a constant-temperature push injection pump and is used for flushing the follicle.

7. The visual double-cavity ovum retrieval system as recited in claim 1, wherein the double-cavity ovum retrieval device further comprises a puncture needle protective sleeve (1) and an oocyte collection tube protective sleeve (4); the puncture needle protective sleeve (1) is used for protecting the puncture needle (2) in the egg taking and packaging process; oocyte collecting pipe protective sheath (4) set up in oocyte collecting pipe (5) with the junction of handle (3) is held to the pjncture needle to avoid buckling and causing oocyte collecting pipe (5) are blockked up, lead to the oocyte to block or lose.

Preferably, the puncture needle holder (3) is in a cylindrical shape with a thin middle part and thick two ends.

More preferably, the oocyte collection tube (5) consists of a multi-layer sleeve formed of a material having a heat exchange medium.

Further preferably, the constant-temperature bolus pump further comprises an adjusting button (601), a foot switch and a power line, wherein the adjusting button is arranged on the surface of the shell (1); the adjustment button (601) is configured to adjust a speed of the bolus card slot (401).

8. The visual dual-lumen egg aspiration system of claim 1, wherein in the negative pressure aspirator, the filter (800) comprises a filter housing and a filter membrane, the filter housing and the filter membrane being in fluid communication with each otherDiameter of the filter membrane

The pore diameter was 0.22. mu.m.

Preferably, the filter shell is made of polypropylene, and the filter membrane is a polytetrafluoroethylene microporous membrane.

More preferably, the first adapter tube (7) has an inner diameter of 6.5mm and an outer diameter of 11 mm.

Preferably, the negative pressure range of the negative pressure aspirator is-10 mmHg to-500 mmHg.

Preferably, the negative pressure aspirator body (100) further comprises a display screen (200), a shell (300) and a sealing ring (400) for sealing the joint of the display screen (200) and the shell (300).

Further preferably, a negative pressure adjusting button is arranged on the display screen (200), and the negative pressure adjusting button comprises a rising negative pressure adjusting key, a falling negative pressure adjusting key and a negative pressure vertical rising key for increasing the negative pressure instantly.

9. A visual double-chamber egg aspiration system according to claim 1, wherein in the thermostat the test tube well (05) is arranged inside the chamber (03) and the chamber (03) is provided with an opening for the insertion of a test tube in correspondence of the top of the test tube well (05).

Preferably, the heating mechanism (04) comprises a heat-generating metal arranged in the base plate (02).

Preferably, the heat-generating metal comprises a wire, a metal tube or a metal block, for example, the heat-generating metal is copper, aluminum, iron.

Preferably, the heating mechanism (04) comprises a heating layer arranged outside the test tube groove (05), and a heat insulation layer is arranged between the heating layer and the test tube groove (05).

Preferably, the heating layer is coated at the middle lower part of the test tube groove (05).

More preferably, the heat generating layer covers at least 1/5 of the test tube slot (05).

10. The visualization double-cavity ovum taking system as recited in claim 9, wherein the heating layer comprises conductive adhesive and carbon fibers, the carbon fibers are distributed inside the conductive adhesive, and the conductive adhesive is connected with an external power supply through a conductive wire and/or conductive cloth.

Preferably, the heat insulation layer is detachably connected to the bottom plate (02).

Preferably, the heating mechanism (04) at the bottom is sleeved on a heat insulation layer, and the heat insulation layer is preferably ceramic.

Preferably, the heating mechanism (04) comprises a plurality of wind heat pipes arranged in the cavity (03), the air inlet ends of the wind heat pipes penetrate through the bottom plate (02) to be connected with an external hot air generator, and the air outlet ends of the wind heat pipes are fixed on the inner wall of the observation window or the side plate (06).

More preferably, the air outlet ends of the plurality of wind heat pipes are inclined towards the center of the chamber, and the inclination angle is 20-35 degrees.

Preferably, temperature sensors are arranged in the cavity and on the outer wall of the test tube groove.

Preferably, the temperature sensor is connected with a temperature controller (07), the temperature controller (07) is used for controlling the temperature of the heating mechanism, and the temperature controller (07) is arranged on the shell or arranged outside the shell.

Preferably, the cross section of the chamber (03) is circular, a regular polygon with sides being greater than or equal to 4 or an anisotropic structure.

Preferably, the chamber (03) has a rectangular, hexagonal or octagonal cross-section, for example rectangular.

Preferably, the chamber (03) is provided with at least one viewing window corresponding to each test tube slot (05), for example two viewing windows corresponding to each test tube slot.

Preferably, the chamber (03) comprises a plurality of side plates (06) connected in sequence, and the observation window is arranged on the side plates (06). More preferably, the side plate (06) is a transparent structure observation window.

Preferably, the top of the chamber (03) is further provided with a top plate (09), and an opening (010) is formed in the top plate (09) corresponding to the top of the test tube groove (05) and used for inserting the test tube into the test tube groove.