CN114145227A - Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage - Google Patents
Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage Download PDFInfo
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- CN114145227A CN114145227A CN202110828387.XA CN202110828387A CN114145227A CN 114145227 A CN114145227 A CN 114145227A CN 202110828387 A CN202110828387 A CN 202110828387A CN 114145227 A CN114145227 A CN 114145227A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method of a rape new variety oil moss 929 for early-maturing and high-yield flowering Chinese cabbage. The invention selects cytoplasmic male sterile line 118A as female parent, self-selects restoring line 927 with crisp and sweet taste, early development and strong resistance as male parent to make hybridization to obtain F1 generation, and makes combined identification, so that it has the characteristics of early development, crisp and sweet taste, good lodging resistance, cold resistance and high yield of flowering cabbage, and its combined 118A is multiplied by 927, i.e. said variety of oil moss 929. The oil moss 929 bred by the breeding method breaks through the single oil function of the traditional rape, expands the vegetable function of the rape, has high fructose content, crisp and sweet taste and good earliness, and compared with the rape with No. 11, the rape with the single oil function has the advantages that the yield of the rape per mu is improved by 800 kilograms of the additive in 80-90 days, the blank of the rape with the single oil function before the spring festival in Hunan province is filled, and the planting benefit of the rape is improved.
Description
Technical Field
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method of a rape new variety oil moss 929 for early-maturing and high-yield flowering Chinese cabbage.
Background
The traditional rape planting mainly aims at harvesting rape seed oil pressing, the yield per mu is about 600-750 yuan, the production cost is removed, the pure benefit per mu is about 100-250 yuan, the planting benefit is low, and the development of rape is seriously influenced. In order to improve the planting efficiency of rape, the development of multifunctional functions of rape for vegetable, fertilizer, feed, appreciation and honey is more and more important in recent years. The rape bolts are emerald green in color, have strong vegetable taste, contain various vitamins and trace elements beneficial to human health, and are novel bolt vegetables. However, the rape bolts in the current market are all from common oil varieties, the time of coming into the market is mainly concentrated in the middle ten days of 2 months, and the time of coming into the market is concentrated; part of the rape bolts are bitter in taste and spicy, and have high cellulose content and thick wax powder. In addition, as the grower basically takes oil collection as the main part and the flowering Chinese cabbage as the auxiliary part, the requirement on the yield of the flowering Chinese cabbage is not high. Therefore, the current benefit of planting the flowering Chinese cabbage is low, and the development of the rape for the flowering Chinese cabbage is influenced.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a breeding method of a new rape seed rape oil moss 929 for precocious high-yield flowering rape, which solves the problems of concentrated time for marketing, poor taste and low yield of the existing rape seed by breeding a new rape seed for precocious high-yield high-resistance flowering rape, promotes the development and utilization of functions of the rape seed and improves the planting benefit of the rape.
In order to achieve the purpose, the invention adopts the following technical scheme:
the breeding method of the rape new variety oil moss 929 for the precocious high-yield flowering Chinese cabbage comprises the following steps:
(1)F0and (3) hybridization: selecting cytoplasmic male sterile line 118A as female parent, and using self-selected restoring line 927 with crisp and sweet taste, early development and strong resistance as male parent to make hybridization so as to obtain F1 generation seed;
the cytoplasmic male sterile line 118A is obtained by hybridizing a sterile line 492A serving as a female parent and a self-selected flowering Chinese cabbage P111 with good taste, multiple branches and thin wax powder serving as a male parent and then performing continuous six-generation backcross and directional breeding;
after the P111 is the rape seed oil 730 is continuously selfed for two generations, the selected individual plants are planted in rows and directionally selected, and finally a multi-branch rape line with light green leaf color and thin wax powder is selected, namely the P111;
the restorer line 927 is obtained by hybridizing the Zhongshuang No. 11 female parent and the early-maturing new strain P4 male parent and performing continuous multi-generation selfing directional breeding;
the precocious new strain P4 is formed by hybridizing a new strain P4 by taking Hui 5 as a female parent and taking Zhongshuang No. 6 as a male parent and then carrying out directional selection for 6 continuous generations;
(2) planting the F1 generation seeds, performing combination identification, and selecting the growth period, the taste, the regeneration capacity, the resistance and the yield, wherein the combination 118A x 927 has the characteristics of early development, crisp and sweet taste, good lodging resistance, cold resistance and high yield of the flowering Chinese cabbage, and the combination 118A x 927 is the variety of the oil moss 929.
Further, in the step (2), the selection of the growth period is carried out by respectively recording the sowing period, the seedling emergence period, the bud period, the bolting period and the bolting period of the main bolt of each combination; selecting the taste of the flowering Chinese cabbage by a method combining field tasting and stir-frying tasting; screening combinations with strong regeneration capacity by counting the number of the continuous 20-plant bolting roots of each combination; the resistance of the rape bolts is selected through investigation of disease resistance and cold resistance; and (4) selecting the yield of the rape bolts by counting the actual harvest yield of the commercial bolts.
Further, the approved number of the sterile line 492A is xiang pin trial 318.
The rich oil 730 is bred by the crop research institute in Hunan province, and the examined serial number is Xiang Ju oil 2008001.
The restoring line 927 is formed by hybridizing and carrying out continuous multi-generation selfing oriented breeding by taking Zhongshuang No. 11 (national audit oil 2008030) bred by the Chinese oil crop research institute as a female parent and an early-maturing new line P4 as a male parent. Wherein P4 is obtained by hybridizing the male parent Hui 5 of Xiang miscellaneous oil No. 3 (Xiang quality inspection No. 317) bred by the agricultural science research institute of Changde city, and Zhongshuang No. 6 (national inspection oil 2003027) bred by the Chinese oil crop research institute, and performing continuous 6-generation directional selection.
Compared with the prior art, the invention has the following beneficial effects:
the rape new variety oil moss 929 bred by the breeding method breaks through the single oil function of the traditional rape, expands the vegetable function of the rape, has high fructose content, crisp and sweet taste and good earliness, and compared with the rape with the double 11 rape, the rape bolts are put on the market 80-90 days earlier, the yield of the rape bolts per mu is improved by 700 plus materials of 800 kg, the blank of the rape bolts before the spring festival in Hunan province is filled, and the planting benefit of the rape is improved.
Drawings
FIG. 1 shows the 118A pedigree for selection.
FIG. 2 shows a 927 pedigree of breeding.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The materials used in the following examples are all commercially available from conventional sources.
EXAMPLE 1 Breeding of cytoplasmic Male sterile line 118A
(1) Breeding targets: has crisp and sweet taste, multiple branches, strong resistance and strong combining ability.
(2) Creating breeding materials: a plant with light green leaf color, thin wax powder and multiple branches is found from a test sample variety (the variety is Feng oil 730 bred by the Hunan province crop research institute in 2004, examined number Xiang Ju oil 2008001), and the quality detection is carried out after the flowering phase bagging selfing in the same year to achieve the double-low standard through fresh taste. In autumn of 2005, the harvested seeds are planted and observed to have separation phenomenon, in spring of 2006, single plants with good taste, multiple branches and thin wax powder of the flowering Chinese cabbage are selected to be bagged for selfing, disease-free plants are harvested in the mature period, and then quality detection is carried out; in autumn of 2006, planting selected single plants in rows, and in spring of 2007, continuing to perform directional selection on each plant row; and continuously planting selected single plants in rows in autumn in 2007, selecting single plants in plant rows with more regular characters in spring in 2008, and harvesting the plants in rows in the mature period. Selecting spring 2009 and spring 2010 according to the same method; and (4) propagating the collected plant rows according to the plant lines in autumn of 2010 to obtain 8 parts of new materials.
(3) And (3) sterile line breeding: in 2011 in spring, 8 parts of new material are respectively hybridized with a sterile line 492A (Xiang Pin Ju No. 318), the combination characteristics are observed in the next year, the corresponding male parent P111 which can keep sterility and has good sterility character is selected, and the subsequent generations are backcrossed continuously. The specific breeding process comprises the following steps:
f0: 2011 spring, 492A hybridizes to P111.
F1: in spring 2012, the single plants with opened petals and degenerated stamens are selected to be backcrossed with P111, and after the plants are mature, the plants are respectively harvested and threshed.
BC1F 1: in 2013, the descendants are planted in rows, during the flowering phase, the single plants with opened petals and degenerated stamens are selected to be backcrossed with the parent books of the previous generation, and after the plants are mature, the plants are harvested and threshed.
BC2F 1: and in summer of 2013, continuing to plant the backcross progeny in rows, selecting single plants to be backcrossed with the parent numbers of the previous generation in the plant rows with the same flowering phase, opened petals, complete sterility and no micro powder in the flowering phase, investigating the outcrossing maturing rate, and selecting the backcross progeny with high outcrossing maturing rate after maturation to harvest and thresh.
BC3F 1: in 2014, in the spring, single plants are selected from the backcross progeny plant rows with regular characters, consistent flowering phase and better sterile characters and are continuously backcrossed with the parent of the previous generation, and after the plants are mature, progeny with good disease resistance, high outcross seed setting rate and excellent economic characters are selected and mixed for threshing.
BC4F 1: in summer of 2014, planting in different branches, and continuing backcross directional selection.
BC5F1 — BC6F 1: selecting according to similar methods in 2015 spring and 2016 spring.
The combined testing and matching are carried out in 2016, and a sterile line with high quality, good taste of the flowering Chinese cabbage, good sterile property, multiple branches and strong testing and matching advantages is bred in 2016, and is named as 118A.
The 118A breeding pedigree is shown in figure 1:
EXAMPLE 2 Breeding of restorer line 927
(1) Breeding targets: the taste is crisp and sweet, the development is early and the resistance is strong.
(2) The breeding process comprises the following steps: the method is characterized by using Zhongshuang No. 11 (national oil 2008030) bred by the Chinese oil crop research institute as a female parent and an early-maturing new strain P4 as a male parent to perform hybridization and continuous multi-generation selfing oriented breeding. Wherein P4 is obtained by hybridizing the male parent Hui 5 of Xiang miscellaneous oil No. 3 (Xiang quality inspection No. 317) bred by the agricultural science research institute of Changde city, and Zhongshuang No. 6 (national inspection oil 2003027) bred by the Chinese oil crop research institute, and performing continuous 6-generation directional selection.
The specific breeding process of the restorer line 927 is as follows:
f0: in spring of 2012, the male parent of Zhongshuang No. 11 and the male parent of P4 were hybridized.
F1: in the spring of 2013, 10 plants were bagged in the F1 generation and mixed after harvesting.
F2: in 2013 summer, sowing the mixed seeds of the previous generation, planting 300 plants, selecting 11 single plants which are early in bolting, good in moss palatability, thick and strong in moss, good in cold resistance and disease resistance, and good in plant leaf type, bagging, and harvesting after maturing.
F3: in autumn of 2013, 11 selected plants in the previous generation are planted into plant rows by single plants, 7 early moss plant rows are selected, single plants meeting the target characters are selected from the 7 early moss plant rows respectively and then bagged, and 18 single plants are selected.
F4: in summer of 2014, 18 selected plants in the previous generation are individually planted into plant rows, 3 plant rows which meet the target character and have high uniformity are selected, individual plant selection is carried out in the selected plant rows, and the plants are harvested and mixed in the individual plant rows after being matured.
F5: in spring 2015, after the individual selection of 3 strains (925, 927 and 929) selected in the previous generation, the strains are divided and reserved.
F6: in summer 2015, the same method is continuously selected for stabilization.
Compared with strains in 2015-2016, 927 is early-maturing, crisp and sweet in taste, and strong in disease resistance, disease resistance and cold resistance. And breeding a premature new line 927.
927 the breeding pedigree is shown in FIG. 2:
EXAMPLE 3 Breeding of oil moss 929
Preparing a hybridization combination by using 118A and a new strain 927 in 2016, carrying out combination identification in 2016 and 2017, respectively recording the sowing period, the emergence period, the bud period, the bolting period and the bolting period of the main bolt of each combination, and selecting the growth period; selecting the taste of the flowering Chinese cabbage by a method combining field tasting and stir-frying tasting; screening combinations with strong regeneration capacity by counting the number of the continuous 20-plant bolting roots of each combination; the resistance condition of each combination is known through the investigation of disease resistance and cold resistance; and finally, counting the actual harvest yield of each combined commodity bolt. By combining the selection, 118A and the new strain 927 are prepared to be hybridized and combined, and the hybrid rice has the advantages of early development, crisp and sweet taste, good disease resistance and cold resistance, high yield and the like.
In order to further understand the combination characteristics of 118A multiplied by 927 (referred to as oil moss 929), rape variety comparison tests for the flowering plants are respectively arranged in five markets of Hunan province in 2017 and 2018, the average yield of the five-spot commercial flowering plants in 2017 and 2018 is 1299.62 kg, the flowering plants have crisp and sweet mouthfeel, the plant regeneration capacity is strong, and the disease resistance and cold resistance are strong; 2018 and 2019 years five-point commercial bolting average yield is 1353.96 kg, the bolting taste is crisp and sweet, the plant regeneration capacity is strong, and the disease resistance and cold resistance are strong.
The double 11 in the variety is used as a contrast, the test adopts a cell test, the area of the cell is 20 square meters, the cell is repeated for three times, and the random block design is adopted. The test results are shown in the table below, and the results show that the oil moss 929 has a short growth period and good earliness, the flowering Chinese cabbage can be listed on the market for 80-90 days earlier, the taste is crisp and sweet, the yield of the flowering Chinese cabbage is high, and the development of the flowering Chinese cabbage industry is promoted.
Claims (2)
1. A breeding method of a new rape oil moss 929 for early-maturing high-yield flowering Chinese cabbage is characterized by comprising the following steps:
(1)F0and (3) hybridization: selecting cytoplasmic male sterile line 118A as female parent, and using self-selected restoring line 927 with crisp and sweet taste, early development and strong resistance as male parent to make hybridization so as to obtain F1 generation seed;
the cytoplasmic male sterile line 118A is obtained by hybridizing a sterile line 492A serving as a female parent and a self-selected flowering Chinese cabbage P111 with good taste, multiple branches and thin wax powder serving as a male parent and then performing continuous six-generation backcross and directional breeding;
after the P111 is the rape seed oil 730 is continuously selfed for two generations, the selected individual plants are planted in rows and directionally selected, and finally a multi-branch rape line with light green leaf color and thin wax powder is selected, namely the P111;
the restorer line 927 is obtained by hybridizing the Zhongshuang No. 11 female parent and the early-maturing new strain P4 male parent and performing continuous multi-generation selfing directional breeding;
the precocious new strain P4 is formed by hybridizing a new strain P4 by taking Hui 5 as a female parent and taking Zhongshuang No. 6 as a male parent and then carrying out directional selection for 6 continuous generations;
(2) planting the F1 generation seeds, performing combination identification, and selecting the growth period, the taste, the regeneration capacity, the resistance and the yield, wherein the combination 118A x 927 has the characteristics of early development, crisp and sweet taste, good lodging resistance, cold resistance and high yield of the flowering Chinese cabbage, and is the variety of the oil moss 929.
2. The breeding method of the new rape seed coat 929 for early-maturing high-yield flowering cabbage according to claim 1, characterized in that in the step (2), the selection of the growth period is performed by respectively recording the sowing period, the emergence period, the bud period, the bolting period and the bolting period of the main bolt of each combination; selecting the taste of the flowering Chinese cabbage by a method combining field tasting and stir-frying tasting; screening combinations with strong regeneration capacity by counting the number of the continuous 20-plant bolting roots of each combination; the resistance of the rape bolts is selected through investigation of disease resistance and cold resistance; and (4) selecting the yield of the rape bolts by counting the actual harvest yield of the commercial bolts.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145225A (en) * | 2021-07-21 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896869A (en) * | 2017-11-02 | 2018-04-13 | 常德市农林科学研究院 | A kind of Early maturity rape tongue high mountain green cultural method |
| CN114145225A (en) * | 2021-07-21 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
| CN114793880A (en) * | 2022-01-19 | 2022-07-29 | 常德市农林科学研究院 | Breeding method of precocious high-yield high-oil high-bolting-resistance oil dual-purpose rape oil moss 928 |
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- 2021-07-22 CN CN202110828387.XA patent/CN114145227A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896869A (en) * | 2017-11-02 | 2018-04-13 | 常德市农林科学研究院 | A kind of Early maturity rape tongue high mountain green cultural method |
| CN114145225A (en) * | 2021-07-21 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
| CN114793880A (en) * | 2022-01-19 | 2022-07-29 | 常德市农林科学研究院 | Breeding method of precocious high-yield high-oil high-bolting-resistance oil dual-purpose rape oil moss 928 |
Non-Patent Citations (1)
| Title |
|---|
| 彭国钢等: "优质高产高抗早熟油菜薹新品种油苔929", 《长江蔬菜》, no. 6, pages 43 - 44 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114145225A (en) * | 2021-07-21 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
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