CN113616158A - Fluorescence excitation device, excitation method and fluorescence endoscope - Google Patents
Fluorescence excitation device, excitation method and fluorescence endoscope Download PDFInfo
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- A—HUMAN NECESSITIES
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- A61B1/042—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances characterised by a proximal camera, e.g. a CCD camera
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- A61B1/043—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
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- A—HUMAN NECESSITIES
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- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
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Abstract
The invention relates to a fluorescence excitation device, an excitation method and a fluorescence endoscope, which comprise a light source, wherein a first filter, a first convergent lens and an incident end of an optical fiber are sequentially arranged along the axial direction irradiated by the light source, the emitting end of the optical fiber is vertically arranged, the emitting end faces to a divergent lens, and the emitting end is superposed with the axial line of the divergent lens; and a second converging lens is arranged on the same horizontal line with the diverging lens, faces the detected body, and is sequentially provided with a second filter, a third converging lens and a view finding surface of the CCD camera along the axis of the second converging lens and the side far away from the detected body. The internal structure of the fluorescence excitation device is reduced, the two sets of filters of the fluorescence excitation device are switched between two horizontal positions and two vertical positions by using the movable rotating shaft, the two sets of filters can be fixed to corresponding positions, one set of device is used for simultaneously realizing shooting of the reference light image and the fluorescence image, and the reference light image generation part and the fluorescence image generation part are combined into one.
Description
Technical Field
The invention relates to the technical field of molecular imaging, in particular to a fluorescence excitation device, an excitation method and a fluorescence endoscope.
Background
The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.
A fluorescence endoscope apparatus: the light source emits excitation light and reference light (white light), which are irradiated to the subject through the optical fiber and the diffusion lens in sequence, and the fluorescence generated by the subject excitation and the reflected excitation light are separated from the reference light by the beam splitter to form two beams of light, which are respectively the mixed light of the fluorescence and the excitation light and the reference light. The reference light is condensed, and an optical image is converted into a digital signal by a color CCD camera of the white light photographing section, and then conducted to the white light image generating section to generate a reference image. After the mixed light of the fluorescence and the exciting light is separated by the beam splitter, the exciting light is filtered by an exciting light cut-off filter (only the fluorescence is transmitted), the transmitted fluorescence is converged, an optical image is converted into a digital signal by a high-sensitivity monochromatic CCD camera of the fluorescence photographing part, and then the digital signal is transmitted to the fluorescence image generating part to generate a fluorescence image. In the image processing, a correction image is obtained by division and correction processing, and this image can display a lesion regardless of the observation condition and the state (color and shape) of the subject, compared to a simple fluorescence image.
The device has a complex structure, needs a large number of repeated parts for superposition inside, has high cost, does not separate monochromatic light from white light, and has an unsatisfactory excitation effect.
Disclosure of Invention
In order to solve the technical problems in the background art, the invention provides a fluorescence excitation device, an excitation method and a fluorescence endoscope, monochromatic purple light separated from white light is used as excitation light through a filter, a better fluorescence excitation effect is achieved, and meanwhile, the position of the filter is adjusted by using a movable rotating shaft, so that a white light source can be used for completing the acquisition of a white light image and a fluorescence image, and the internal structure is simplified.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a fluorescence excitation device of a fluorescence endoscope, which comprises a light source, a first filter, a first convergent lens and an incident end of an optical fiber, wherein the first filter, the first convergent lens and the incident end of the optical fiber are sequentially arranged along the axial direction irradiated by the light source; and a second converging lens is arranged on the same horizontal line with the diverging lens, faces the detected body, and is sequentially provided with a second filter, a third converging lens and an image acquisition module along the axis of the second converging lens and on one side far away from the detected body.
The light source is arranged along the horizontal direction, the incident end of the optical fiber is horizontally arranged, the emission end is vertically arranged, and the object to be detected is positioned in the space below the divergent lens and the second convergent lens.
One side of the first filter is movably connected with the shell through a rotating shaft, so that the switching between the horizontal direction and the vertical direction is realized; the horizontal direction is parallel to and higher than the light rays emitted by the light source; the axis in the vertical direction coincides with the light ray.
One side of the second filter is movably connected with the shell to realize the switching between the vertical direction and the horizontal direction; the light rays passing through the second converging lens are parallel to each other in the vertical direction; the horizontal direction coincides with the axis of the light rays passing through the second condenser lens.
The first filter is a purple filter, and the first filter in the vertical direction filters light emitted by the light source into purple monochromatic light.
The second filter is a cut-off filter, and the second filter in the horizontal direction filters monochromatic light for exciting fluorescence so as to transmit the reflected fluorescence.
The second aspect of the present invention provides a fluorescence excitation method based on the above device, comprising the steps of:
enabling the first filter to be in the horizontal direction, enabling the second filter to be in the vertical direction, starting a light source, shooting for the first time, and obtaining a reference image;
and enabling the first filter to be in the vertical direction and the second filter to be in the horizontal direction, starting the light source, shooting for the second time, and obtaining a fluorescence image.
The first shooting specifically comprises the following steps: the first filter is in the horizontal direction, light rays emitted by the light source enter the optical fibers after passing through the first converging lens without passing through the first filter, and the light rays irradiate the detected body after being emitted by the optical fibers;
the light rays are reflected by the detected body, the second filter is in the vertical direction, the reflected light rays do not pass through the second filter, all the reflected light rays are converged by the second converging lens and the third converging lens in sequence, then the light rays enter the image acquisition module to convert the optical image into digital signals, and the digital signals are transmitted to the image generation part to generate the reference image.
The second shooting specifically comprises: the first filter is in the vertical direction, the light rays pass through the first filter to obtain monochromatic light, and the monochromatic light enters the optical fiber after being converged by the first converging lens and is transmitted to the diverging lens 5 to irradiate on the detected body;
the detected body is excited by monochromatic light to emit fluorescent light, meanwhile, the detected body reflects the light, the second filter is located in the horizontal direction, the second filter filters the fluorescent light emitted by the detected body, only the reflected light passes through the second filter to realize transmission, and then the reflected light is converged by the third converging lens and enters the image acquisition module to convert the optical image into a digital signal, and the digital signal is transmitted to the image generation part to generate a fluorescent image.
A third aspect of the present invention provides a fluorescence endoscope including an image generating section and an image processing section, wherein the image generating section has the fluorescence excitation device described above.
Compared with the prior art, the above one or more technical schemes have the following beneficial effects:
1. the internal structure of the fluorescence excitation device is reduced, the two sets of filters of the fluorescence excitation device are switched between two horizontal positions and two vertical positions by using the movable rotating shaft, the two sets of filters can be fixed to corresponding positions, one set of device is used for simultaneously realizing shooting of the reference light image and the fluorescence image, and the reference light image generation part and the fluorescence image generation part are combined into one.
2. The purple light is used as exciting light, and the wavelength of the purple light is 400-435nm, which is shorter than the wavelength of the white light used by the original device and containing the exciting light, is 400-740nm, so that the energy is larger and the exciting effect is good.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a schematic diagram of a first filter action provided by one or more embodiments of the present invention;
FIG. 2 is a schematic diagram of a second filter action provided by one or more embodiments of the invention;
fig. 3 is a schematic layout view of monitoring points for horizontal displacement of side walls of a track section according to one or more embodiments of the present invention;
in the figure: 1. the CCD camera comprises a light source, 2, a first filter, 3, a first converging lens, 4, an optical fiber, 5, a diverging lens, 6, a second converging lens, 7, a second filter 8, a third converging lens, 9 and a CCD camera.
Detailed Description
The invention is further described with reference to the following figures and examples.
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
The principle of fluorescence-excited imaging can be described as: when external light irradiates biological tissues (detected bodies) with fluorophores, the fluorophores absorb light energy to enable electrons to transition to an excited state, the electrons release fluorescence in the process of returning to the ground state from the excited state, the fluorescence moves towards the red end compared with the absorbed light, namely, the emitted fluorescence has lower energy than the absorbed external light, the fluorescence propagates in the tissues and partially reaches the body surface, and the fluorescence emitted from the body surface is received by a detector, so that a fluorescence image is formed.
In practical applications, it is necessary to acquire a white light image (reference light) and a fluorescence image and then to perform image processing to reflect a lesion region of a biological tissue (subject), for example, in cancer screening. The excitation light must be monochromatic light, and the reference light is usually white, so the current fluorescence excitation device and fluorescence endoscope have complex structures, and the interior thereof contains a plurality of components with functions of filters, light sources and the like, so the following embodiments propose the fluorescence excitation device, the excitation method and the fluorescence endoscope, and use monochromatic purple light separated from white light by the filters as the excitation light to achieve better fluorescence excitation effect, and simultaneously use the movable rotating shaft to adjust the positions of the filters, so that the white light source can be used to complete the acquisition of white light images and fluorescence images, thereby simplifying the internal structure.
The first embodiment is as follows:
as shown in fig. 1-3, the fluorescence excitation device includes a light source 1, a first filter 2, a first convergent lens 3 and an incident end of an optical fiber 4 are sequentially arranged along an axial direction irradiated by the light source 1, an emission end of the optical fiber 4 is vertically arranged, the emission end faces a divergent lens 5, and the emission end coincides with an axial line of the divergent lens 5; a second converging lens 6 is arranged on the same horizontal line with the diverging lens 5, the second converging lens 6 faces the subject, and a second filter 7, a third converging lens 8 and a viewing surface of the image acquisition module are sequentially arranged along the axis of the second converging lens 6 and on the side far away from the subject.
The light source 1 is arranged along the horizontal direction, the incident end of the optical fiber 4 is horizontally arranged, the emission end is vertically arranged, and the object to be detected is positioned in the space below the divergent lens 5 and the second convergent lens 6.
One side of the first filter 2 is movably connected with the shell through a rotating shaft, so that the switching between the horizontal direction and the vertical direction is realized; the horizontal direction is parallel to and higher than the light rays emitted by the light source 1; the axis in the vertical direction coincides with the light ray.
One side of the second filter 7 is movably connected with the shell to realize the switching between the vertical direction and the horizontal direction; vertically parallel to the light rays passing through the second condenser lens 6; horizontally downward to coincide with the axis of the rays passing through the second converging lens 6.
The light source 1 emits white light.
The first filter 2 is a purple filter, and the first filter 2 in the vertical direction filters white light emitted by the light source into monochromatic purple.
The second filter 7 is a cut-off filter, and the monochromatic light for exciting the fluorescence is filtered by the second filter 7 in the horizontal direction, so that the reflected fluorescence is transmitted.
A xenon lamp is used as a light source 1 and can emit white light (reference light), a first filter 2 arranged behind the xenon lamp is purple, and two positions are adjusted through a movable rotating shaft, wherein one position is horizontally higher than the light emitted by the light source 1; the second is in the vertical direction, and the central axis of the second is superposed with the light.
The white light is used for shooting images for the first time, the first filter 2 is adjusted to be in the horizontal direction, light rays do not pass through the first filter 2, but enter the incident end of the optical fiber 4 after being converged by the first converging lens 3, and the optical fiber 4 transmits the white light to the diverging lens 5 towards which the emitting end faces to irradiate the detected body.
The white light is reflected by the detected object, and after being converged by the second converging lens 6, the reflected white light enters the image acquisition module after sequentially passing through the second converging lens 6 and the third converging lens 8 without passing through the second filter 7.
And the purple light is used for shooting an image for the second time, the first filter 2 is in the vertical direction, and the light rays are converged by the first converging lens 3, enter the optical fiber 4 and are transmitted to the diverging lens 5 to irradiate the detected body.
Excited fluorescence and reflected excitation light are generated by the excitation of the detected body, the excited fluorescence and the excitation light pass through a second filter 7 in the horizontal direction after passing through a second converging lens 6, the second filter 7 blocks the reflected excitation light and only allows the fluorescence emitted by the excited detected body to pass through, the fluorescence enters a view finding surface of a CCD camera 9 after being converged by a third converging lens 8, the CCD camera 9 converts an acquired optical image into a digital signal, and the digital signal is transmitted to an image generation part to generate a fluorescence image.
As shown in fig. 2, the first filter 2 takes a picture in the horizontal direction for the first time, and takes a picture in the vertical direction for the second time; in the horizontal direction, the first filter 2 is parallel to and higher than the light emitted by the light source 1; in the vertical direction, the axis of the first filter 2 coincides with the light.
For example, the housing is connected to one side of the first filter 2 through a movable rotation shaft, thereby adjusting the position of the first filter 2: the first filter 2 is changed from the vertical direction to the horizontal direction by rotating 90 degrees along the movable rotating shaft.
As shown in fig. 3, the second filter 7 takes a picture in the vertical direction for the first time and in the horizontal direction for the second time; in the vertical direction, the second filter 7 is parallel to the light passing through the second converging lens 6; horizontally down, the second filter 7 coincides with the axis of the rays passing through the second converging lens 6.
For example, the housing is connected to one side of the second filter 7 through a movable rotation shaft, thereby adjusting the position of the second filter 7: the second filter 7 is rotated by 90 degrees along the movable rotating shaft, and the vertical direction is changed into the horizontal direction.
The movable rotating shaft is connected with the driving element and is fixed through the locking structure after rotating to a set position; the structural types of the drive element and the locking structure are not restricted.
The structure reduces the internal structure of the fluorescence excitation device: by using the movable rotating shaft, the filter 2 and the filter 7 can be switched between a horizontal position and a vertical position and can be fixed to corresponding positions, and a set of device is utilized to simultaneously realize the shooting of the reference light image and the fluorescence image, so that a beam splitter, a convergent lens and a CCD are omitted, and the reference light image generation part and the fluorescence image generation part are combined into a whole.
The purple light is used as exciting light, and the wavelength of the purple light is 400-435nm, which is shorter than the wavelength of the white light used by the original device and containing the exciting light, is 400-740nm, so that the energy is larger and the exciting effect is good.
Example two:
the present embodiment provides a fluorescence excitation method based on the above embodiments, including the following steps:
enabling the first filter 2 to be in the horizontal direction and the second filter 7 to be in the vertical direction, starting the light source 1, shooting for the first time, and obtaining a reference image;
enabling the first filter 2 to be in the vertical direction and the second filter 7 to be in the horizontal direction, starting the light source 1, and shooting for the second time to obtain a fluorescence image;
the first shooting is carried out, the first filter 2 is in the horizontal direction, light rays emitted by the light source 1 enter the optical fibers 4 after passing through the first converging lens 3 without passing through the first filter 2, and the light rays irradiate a detected body after being emitted by the optical fibers;
the light rays are reflected by the detected body, the second filter 7 is in the vertical direction, the reflected light rays do not pass through the second filter 7, all the reflected white light is converged by the second converging lens 6 and the third converging lens 8 in sequence, then enters the CCD camera 9 to convert the optical image into a digital signal, and the digital signal is transmitted to the image generating part to generate a reference image.
The second shooting is carried out, the first filter 2 is in the vertical direction, the light rays pass through the first filter 2 to obtain monochromatic light, and the monochromatic light enters the optical fiber 4 after being converged by the first convergent lens 3 and is transmitted to the divergent lens 5 to irradiate the detected body;
the detected body is excited by monochromatic light to emit fluorescent light, meanwhile, the detected body reflects the light, the second filter 7 is located in the horizontal direction, the second filter 7 filters the fluorescent light excited by the detected body, only the reflected optical fiber passes through the second filter 7 to realize transmission, the optical fiber is converged by the third converging lens 8 and enters the CCD camera 9 to convert the optical image into a digital signal, and the digital signal is transmitted to the image generating part to generate a fluorescent image.
The above process enables the filter 2 and the filter 7 to be switched between the horizontal position and the vertical position, and can be fixed to the corresponding positions, and one set of device is utilized to simultaneously realize the shooting of the reference light image and the fluorescence image, thereby saving a complex structure.
Example three:
the present embodiment provides a fluorescence endoscope including an image generating section and an image processing section, wherein the image generating section has the fluorescence excitation device described in the first embodiment.
The fluorescence endoscope has an excitation light source, a filter, an optical fiber, a diverging lens, a converging lens, a CCD camera, and a computer system.
The structure enables the filter 2 and the filter 7 in the fluorescence excitation device to be switched between a horizontal position and a vertical position, and can be fixed to corresponding positions, and one set of device is utilized to simultaneously realize the shooting of reference light images and fluorescence images, so that the complex internal structure of the fluorescence endoscope is omitted.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. Fluorescence excitation device, its characterized in that: the device comprises a light source, wherein a first filter, a first convergent lens and an incident end of an optical fiber are sequentially arranged along the axial direction irradiated by the light source, the emitting end of the optical fiber is vertically arranged, the emitting end faces a divergent lens, and the emitting end is superposed with the axial line of the divergent lens; and a second converging lens is arranged on the same horizontal line with the diverging lens, faces the detected body, and is sequentially provided with a second filter, a third converging lens and an image acquisition module along the axis of the second converging lens and on one side far away from the detected body.
2. Fluorescence excitation device according to claim 1, characterized in that: the light source is arranged along the horizontal direction, the incident end of the optical fiber is horizontally arranged, the emitting end is vertically arranged, and the object to be detected is positioned in the space below the divergent lens and the second convergent lens.
3. Fluorescence excitation device according to claim 1, characterized in that: one side of the first filter is movably connected with the shell through a rotating shaft, so that the switching between the horizontal direction and the vertical direction is realized; the horizontal direction is parallel to the light emitted by the light source and is higher than the light, and the vertical direction axis coincides with the light.
4. Fluorescence excitation device according to claim 1, characterized in that: one side of the second filter is movably connected with the shell to realize the switching between the vertical direction and the horizontal direction; the vertical direction is parallel to the light rays passing through the second converging lens, and the horizontal direction is coincident with the axis of the light rays passing through the second converging lens.
5. Fluorescence excitation device according to claim 3, characterized in that: the first filter is a purple filter, and the first filter in the vertical direction filters light emitted by the light source into purple monochromatic light.
6. Fluorescence excitation device according to claim 4, characterized in that: the second filter is a cut-off filter, and the second filter in the horizontal direction filters monochromatic light for exciting fluorescence so as to transmit the reflected fluorescence.
7. Fluorescence excitation method based on the device according to any one of claims 1 to 6, comprising the following steps:
the first filter is in the horizontal direction, the second filter is in the vertical direction, a light source is started, and first shooting is carried out to obtain a reference image;
the first filter is in the vertical direction, the second filter is in the horizontal direction, the light source is started, the second shooting is carried out, and the fluorescence image is obtained.
8. The fluorescence excitation device for a fluorescence endoscope according to claim 7, wherein: the first shooting specifically comprises: the first filter is in the horizontal direction, light rays emitted by the light source enter the optical fibers after passing through the first converging lens without passing through the first filter, and the light rays irradiate the detected body after being emitted by the optical fibers;
the light rays are reflected by the detected body, the second filter is in the vertical direction, the reflected light rays do not pass through the second filter, all the reflected light rays are converged by the second converging lens and the third converging lens in sequence, then the light rays enter the image acquisition module to convert the optical image into digital signals, and the digital signals are transmitted to the image generation part to generate the reference image.
9. The fluorescence excitation device for a fluorescence endoscope according to claim 7, wherein: the second shooting specifically comprises: the first filter is in the vertical direction, the light rays pass through the first filter to obtain monochromatic light, and the monochromatic light enters the optical fiber after being converged by the first converging lens and is transmitted to the diverging lens to irradiate on the detected body;
the detected body is excited by monochromatic light to emit fluorescent light, meanwhile, the detected body reflects the light, the second filter is located in the horizontal direction, the second filter filters the fluorescent light emitted by the detected body, only the reflected light passes through the second filter to realize transmission, and then the reflected light is converged by the third converging lens and enters the image acquisition module to convert the optical image into a digital signal, and the digital signal is transmitted to the image generation part to generate a fluorescent image.
10. A fluorescence endoscope comprising an image generating section and an image processing section, wherein the image generating section has the fluorescence excitation device according to any one of claims 1 to 6.
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| CN103687529A (en) * | 2011-07-22 | 2014-03-26 | 奥林巴斯株式会社 | Fluorescence endoscopy system |
| CN109068968A (en) * | 2016-09-16 | 2018-12-21 | 奥林巴斯株式会社 | Light source device for endoscope |
| CN112043240A (en) * | 2019-06-06 | 2020-12-08 | 卡尔史托斯股份有限公司 | Light source, system for fluorescence diagnosis and method for fluorescence diagnosis |
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2021
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20020175993A1 (en) * | 2001-05-16 | 2002-11-28 | Olympus Optical Co., Ltd. | Endoscope system using normal light and fluorescence |
| US20050027166A1 (en) * | 2003-06-17 | 2005-02-03 | Shinya Matsumoto | Endoscope system for fluorescent observation |
| CN103687529A (en) * | 2011-07-22 | 2014-03-26 | 奥林巴斯株式会社 | Fluorescence endoscopy system |
| CN109068968A (en) * | 2016-09-16 | 2018-12-21 | 奥林巴斯株式会社 | Light source device for endoscope |
| CN112043240A (en) * | 2019-06-06 | 2020-12-08 | 卡尔史托斯股份有限公司 | Light source, system for fluorescence diagnosis and method for fluorescence diagnosis |
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