CN112871029B - Sample adding and mixing method, sample adding and mixing device, computer storage medium, sample analysis method and sample analysis device - Google Patents

Sample adding and mixing method, sample adding and mixing device, computer storage medium, sample analysis method and sample analysis device Download PDF

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Publication number
CN112871029B
CN112871029B CN201911206958.5A CN201911206958A CN112871029B CN 112871029 B CN112871029 B CN 112871029B CN 201911206958 A CN201911206958 A CN 201911206958A CN 112871029 B CN112871029 B CN 112871029B
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China
Prior art keywords
sample
placing
mixing
placing part
state
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Chinese (zh)
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CN112871029A (en
Inventor
赖华忠
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Shenzhen Dymind Biotechnology Co Ltd
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Shenzhen Dymind Biotechnology Co Ltd
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Priority to CN201911206958.5A priority Critical patent/CN112871029B/en
Priority to PCT/CN2020/132465 priority patent/WO2021104499A1/en
Publication of CN112871029A publication Critical patent/CN112871029A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/712—Feed mechanisms for feeding fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/7174—Feed mechanisms characterised by the means for feeding the components to the mixer using pistons, plungers or syringes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials

Abstract

The invention discloses a sample adding and mixing method, a sample adding and mixing device, a computer storage medium, a sample analysis method and a sample analysis device, wherein the sample adding and mixing method comprises the following steps: placing the sample placing part on a suspension position; the sample adding part adds a substance to be added to the sample placing part; uniformly mixing the sample placing parts through a transferring part; the sample placing unit is configured to have at least one of a moving state and a kneading state by the transfer unit. The invention can effectively shorten the time from the mixing link to the detection link.

Description

Sample adding and mixing method, sample adding and mixing device, computer storage medium, sample analysis method and sample analysis device
Technical Field
The invention relates to the technical field of medical examination, in particular to a sample adding and mixing method, a sample adding and mixing device, a computer storage medium, a sample analysis method and a sample analysis device.
Background
In the technical field of medical examination, adding a sample and a reagent into a sample placing part for reaction, detection, processing and/or analysis, and uniformly mixing the sample and the reagent is a common application scenario. Wherein, the blending operation is a key link of the process.
The existing blending modes mainly comprise the following two modes:
1. the blending mechanism is arranged in the placing area of the sample placing part, and after sample adding operation of a sample and a reagent is completed, blending is directly carried out on the placing area, and then detection operation of the sample is carried out at a detection position. Because the sample placing part needs to move from the placing area to the detection position after the uniform mixing, the required time is long, the optimal detection time of the sample is easy to miss, and particularly, the sample detection process with fast reaction is realized. This blending mode adversely affects both inspection speed and performance.
2. The blending mechanism is arranged at the detection position of the sample, and is transferred to the detection position after completing the sample adding operation of the sample and the reagent, and is blended at the detection position. The structure of the mixing mode is relatively complex, and for the unified detection and analysis of a plurality of samples, a mixing mechanism needs to be added on each detection channel, so that the cost is obviously increased.
How to provide a blending mode which can shorten the time from the blending link to the detection link and does not need to increase the cost, and an effective solution scheme does not exist in the prior art at present.
Disclosure of Invention
Aiming at the problems, the invention develops a sample adding and mixing method and a sample analysis method which can effectively shorten the time from a mixing link to a detection link, and also provides a sample adding and mixing device, a sample analysis device and a computer storage medium which can realize the methods.
The invention adopts a technical means that: the sample adding and mixing method comprises the following steps:
placing the sample placing part on a suspension position;
the sample adding part adds a substance to be added to the sample placing part;
uniformly mixing the sample placing parts through a transferring part; under the action of the transfer part, the sample placing part has at least one state of a moving state and a blending state; the position of the sample placing part in the blending state is the suspended position, or any position which can be passed by the suspended position in the process of moving to the detection position.
The invention adopts another technical means that: the utility model provides a sample application mixing device, includes:
the sample adding part is used for absorbing the object to be added and adding the sample into the sample placing part;
a first sample adding driving part for driving the movement of the sample adding part;
the second sample adding driving part is used for driving the sample adding part to realize the absorption or addition operation of the object to be added;
the transfer part is used for moving and uniformly mixing the sample placing part;
a control unit for controlling the first sample application driving unit, the second sample application driving unit, and the transfer unit;
the control unit includes a processor and a memory, and the memory stores a computer program that, when executed by the processor, causes the processor to execute the sample-adding and mixing method described above.
The invention adopts another technical means that: there is provided a computer storage medium storing a computer program which, when executed by a processor, causes the processor to execute the sample application and homogenization method as described above.
According to the sample adding and mixing method, the sample placing part is placed on the suspension position, then the object to be added is added to the sample placing part, and then the sample placing part is mixed uniformly through the transferring part. The position for uniformly mixing the sample placing parts can be the suspended position or any position which can be passed by the sample placing parts in the uniform mixing state in the process of moving the sample placing parts from the suspended position to the detection position, so that the sample adding and uniformly mixing method can be realized without additionally arranging a uniformly mixing mechanism in a sample placing part or the detection position, and the cost can be effectively reduced; meanwhile, the sample placing part positioned in the suspension position is uniformly mixed and moved after sample adding, so that the time for moving the sample placing part to the detection position is reduced, the problem of missing of the optimal detection time after mixing is avoided, and the detection efficiency and the accuracy of the detection result are improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Wherein:
FIG. 1 is a flow chart of a sample application and mixing method in one embodiment;
FIG. 2 is a flow chart of a sample application and homogenization method in one embodiment;
FIG. 3 is a flow chart of a sample application and homogenization method in one embodiment;
FIG. 4 is a flowchart showing a procedure of loading the sample by the loading part into the sample loading part in one embodiment;
FIG. 5 is a flowchart of a sample application and mixing method in one embodiment;
FIG. 6A, FIG. 6B, FIG. 6C and FIG. 6D are exemplary illustrations of a sample-adding and mixing method;
FIG. 7 is a view showing an example of moving the sample-placing section to the detection position;
FIG. 8 is a diagram illustrating an embodiment of moving a sample placement portion from a placement position to a hanging position;
FIG. 9 is a block diagram of the sample-adding and mixing device in one embodiment.
In the figure: 1. placing position, 2, sample placing part, 3, suspension position, 4, detection position, 5, sample adding part, 6, transfer part, 7 and liquid level.
Detailed Description
In order to make the objects, technical solutions and technical effects of the present invention more clear, the present invention is further described in detail below with reference to the accompanying drawings and the detailed description. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention. The embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
The invention provides a sample adding and mixing method, as shown in fig. 1, in one embodiment, the sample adding method can comprise the following steps:
step S2: the sample-placing part is placed in a suspended position. Under the condition that the sample placing part is positioned at the suspension position, the sample placing part is in a suspension state;
the sample placing part refers to a container for placing a sample or a reagent, and may be a sample container, a reagent container, such as a reaction cup, a test tube, and the like. The specific type and form of the sample placing part do not affect the sample adding and mixing method claimed in the present application. The sample placing part is in a suspended state, namely the bottom of the sample placing part is not placed on any bearing surface, and the sample placing part positioned on the suspended position can be clamped or fixed by the transferring part, so that the sample placing part is kept in the suspended state. The transfer part can be a component or a structure with a fixing function or a clamping function, such as a mechanical arm, a finger cylinder, a clamping jaw and the like; in one embodiment, the flying position may be above the placement position, above the detection position, or between the placement position and the detection position. The placing position may be a position where the sample placing part is placed before the sample placing part is placed at the suspended position. For example, in the sample analysis, the placing position may be a position for placing an empty sample placing part, or may be a pre-loading position, that is, one or a part of the sample or the reagent is first loaded into the sample placing part at the placing position, and the sample placing part to which the sample or the reagent is loaded at the pre-loading position may be incubated before being moved to the suspending position. The suspension position may be a position convenient for the sample loading part to reach and also convenient for the sample loading part to move to the detection position, for example, the selection of the suspension position may be determined according to the distance between the suspension position and the detection position, the distance between the suspension position and the initial position of the sample loading part, and specifically, may be the suspension position corresponding to the case where the sum of the three distances is the shortest. The detection position may be a position where the sample holder can be placed and an analyte in the sample holder can be detected, the analyte may be an analyte to be added to the sample holder in step S3, a sample or a reagent contained in the sample holder before step S3, or a substance obtained by reacting the analyte with another sample or a reagent in the sample holder.
Step S3: the sample adding part adds a substance to be added to the sample placing part.
The sample addition part may be a sample needle or another member capable of aspirating a sample or a reagent. The substance to be added may be a sample, a reagent, or both a sample and a reagent, that is, in step S3, the sample may be added to the sample placement portion by the sample addition portion, or only a reagent, or both a sample and a reagent are added, if only a sample is added, the reagent needs to be added when or before step S4 is performed, and if only a reagent is added, the sample needs to be added before step S3 is performed, for example, the sample may be added to the sample placement portion in the placement region of the sample placement portion, and then the sample placement portion is moved to the suspended position. Of course, compared with the mode that the sample loading part is moved in front and the sample loading part is moved in back, the mode that the sample loading part is moved in front and the sample loading part is moved in back reduces the moving time of the sample loading part which is loaded, and is beneficial to reducing the occurrence of sample pollution. When the sample adding and mixing method is applied to sample analysis, the sample can be a blood-related sample, such as blood, blood components and the like, and the blood components can be plasma, blood cells and the like; the reagent can be a detection reagent for fibrinolysis, anti-fibrinolysis, blood coagulation, anticoagulation and the like. The sample addition part may add the sample to be added to the sample placing part in a moving state, or may add the sample to be added to the sample placing part in a suspended state, that is, when the sample addition part adds the sample to the sample placing part, the sample addition part and the sample placing part may form a relative motion state, a relative rest state, or an absolute rest state.
Step S4: the sample placing part is mixed by the transferring part. Under the action of the transfer part, the sample placing part has at least one state of a moving state and a blending state;
as described above, the transfer unit may be a member or a structure having a gripping function, such as a robot, a finger cylinder, or a gripper. The sample placing part can be driven to be in a moving state, for example, the sample placing part is transferred to the suspension position and the detection position, or the sample placing part can be driven to be in a uniform mixing state, and then the object to be added in the sample placing part is uniformly shaken, for example, a sample and/or a reagent are uniformly mixed, and when the sample adding and mixing method is applied to sample analysis, a blood-related sample and a detection reagent can be uniformly shaken. Of course, the sample placing unit may be in a moving state and a mixing state by the transfer unit, and mixing may be performed while moving, for example. The sample placement unit may be kept at the suspended position for a predetermined time period by the transfer unit, and the predetermined time period may be a time period required to perform step S3 or step S4.
In one embodiment, the position of the sample placing part in the blending state may be the suspended position, or any position that the sample placing part can pass through when moving from the suspended position to the detection position. The position of the sample placing part for mixing can be the suspension position, under the condition, the sample adding part also directly adds the substance to be added to the sample placing part positioned on the suspension position, and the sample adding part is directly used for mixing at the suspension position after sample adding. The position where the sample placing part is specifically mixed may be any position where the sample placing part can pass when moving from the suspended position to the detection position, that is, the sample placing part may be mixed when the transfer part transfers the sample placing part from the suspended position to the detection position, and there may be a plurality of movement paths from the suspended position to the detection position as long as the sample placing part can be moved from the suspended position to the detection position. In addition, the sample placing part can continuously keep a moving state in the moving process, and certainly, the sample placing part can move for a certain distance, then stops moving, and then continues to enter the moving state after stopping for a certain time. The moving path and the sample placing part can be set according to the requirement in the moving process. For example, the sample placing part can reach the detection position through the suspension position via the first moving path, can also reach the detection position via the second moving path, and can also reach the detection position via other moving paths, and any point position on any moving path can be the position where the sample placing part is uniformly mixed, and of course, the position can be above the detection position where the sample placing part can be accommodated. The implementation position of the blending operation can be determined according to the optimal detection time of the object to be added.
In one embodiment, step S4 may be: the transfer part applies driving force which can enable the sample placing parts to execute blending action to the sample placing parts clamped by the transfer part; the driving force comprises at least one of rotating force, swinging force and vibrating force, and then the sample placing part realizes the action modes of rotating, swinging, vibrating and mixing and other mixing actions. Of course, the rotating force, the swinging force and the vibrating force applied by the transferring part can be superposed or sequentially generated, for example, the sample placing part performs rotating blending and performs vibrating blending. Meanwhile, the duration of the blending action can be adjusted according to the actual situation.
In the sample adding and mixing method in this embodiment, the sample placing portion is first placed in a suspended position, then the substance to be added is added to the sample placing portion, and then the sample placing portion is mixed by the transferring portion. The position for uniformly mixing the sample placing parts can be the suspended position or any position which can be passed by the suspended position in the process of moving the sample placing parts to the detection position, so that the sample adding and uniformly mixing method can be realized without additionally arranging a uniformly mixing mechanism in a sample placing part placing area or the detection position, and the cost can be effectively reduced; meanwhile, the sample placing part positioned at the suspension position is uniformly mixed and moved after sample adding, so that the time for the sample placing part to move to the detection position is reduced, the problem of missing of the optimal detection time after uniform mixing is avoided, and the detection efficiency and the accuracy of the detection result are improved. When the sample adding and mixing method is applied to sample analysis, such as a chemiluminescence analyzer, a coagulation analyzer and other clinical examination equipment, the problems of missing of optimal sample analysis and detection time can be effectively avoided on the basis of controlling cost, and more accurate sample analysis results can be obtained. Under the condition that the position of the sample placing part in the uniform mixing state is the suspended position or the suspended position and the position between the detection positions, the sample placing part is suspended and uniformly mixed, so that the problem that the sample placing part collides with a bearing surface of the sample placing part can be effectively solved.
In one embodiment, as shown in fig. 2 and fig. 3, after the step of adding the sample to be added to the sample placing part by the sample adding part, the method for mixing and adding samples can further include the following steps:
step St: the transfer unit moves the sample placement unit to the detection position.
Specifically, as shown in fig. 2, the step St may be performed after step S4, that is, after the sample placing portion moved to the detection position has completed the operations of adding the sample to be added and mixing the sample to be added, the sample placing portion is directly moved to the detection position for sample detection. Specifically, as shown in fig. 3, the step St may be performed between the step S3 and the step S4, that is, the sample placement unit transferred to the detection position has been loaded with the sample to be loaded but is not subjected to the kneading operation, and after the sample placement unit is transferred to the detection position, the sample placement unit is first kneaded by the transfer unit, and then the sample detection is performed. The two modes can be selected according to the actual sample reaction and analysis requirements.
In one embodiment, the sample adding and mixing method further comprises the following steps:
detecting whether the sample placing part is placed on the transferring part; specifically, it is possible to detect whether or not the sample placement unit is held between the transfer units by a sensor, a detection member, or the like.
Acquiring the current position of the sample placement part when the sample placement part is placed on the transfer part; for example, the current position of the sample placement portion can be known as the suspended position, the detection position, and the like, and specifically, the current position of the sample placement portion can be obtained by detecting the specific position of the transfer portion holding the sample placement portion. When the set sample part is not placed on the transfer part, the transfer part may be controlled to perform the gripping operation of the set sample part.
Controlling the sample placing part to be in the state by the transfer part according to the current position of the sample placing part; through the detection and the acquisition result, the state of the sample placing part is convenient to control and adjust, such as controlling the sample placing part to be in a suspended state, a moving state, a uniform mixing state and the like. For example, when a sample placement unit is held between the transfer units but does not reach the suspended position, the transfer units can control the sample placement unit to be in the moving state. When the sample placing part is clamped on the transferring part and reaches the suspension position, the sample placing part can be controlled to be in the suspension state, the sample adding part can be controlled to carry out sample adding work, and after the preset time, the sample placing part can be controlled to enter a uniform mixing state or a moving state and the like.
In one embodiment, as shown in fig. 4, the step of adding the substance to be added to the sample placing part by the sample adding part may include:
step S32: the sample adding part and the sample placing part are close to each other.
The sample placing part before sample addition may be an empty sample placing part, that is, no liquid such as a sample or a reagent is contained in the sample placing part before sample addition, or a liquid may be contained in the sample placing part before sample addition, that is, a sample or a reagent is added to the sample placing part with the liquid. Before sample adding, the sample adding part can move to the position of the sample placing part, and the position of the sample placing part can be the suspension position; the sample placing part may be moved toward the sample placing part, or the sample placing part and the sample placing part may be moved relatively to each other to be brought close to each other.
Step S34: the sample addition part adds a sample to be added to the sample placement part at a sample addition position. The sample addition position refers to a position where the sample addition part is located when the sample to be added is added, and the sample addition position can be set in advance.
The object to be added may be a sample, a reagent, or the like, and if the sample addition part is a sample needle, the object to be added may be discharged from a sample discharge port of the sample needle.
Step S36: the sample adding part and the sample placing part are far away from each other. After the sample addition, the sample addition part may be moved away from the sample placing part, the sample placing part may be moved away from the sample addition part, or the sample addition part and the sample placing part may be moved away from each other by relative movement.
As shown in FIGS. 6B and 6C, the sample addition part 5 is moved into the sample placement part 2 placed in the suspended position 3 to add a sample.
In one embodiment, the sample addition part at the sample addition position can be in a first state or a second state between the sample addition part and the liquid surface in the sample addition part after the object to be added is discharged; the first state is that the liquid contact position on the sample adding part just contacts the liquid surface in the sample placing part; and the second state is that the liquid contact position on the sample adding part is positioned above the liquid level in the sample placing part, and the distance between the liquid contact position and the liquid level in the sample placing part is less than the preset height. As shown in FIG. 6B, after the sample-adding part 5 discharges the sample to be added, the contact position on the sample-adding part 5 is located above the liquid surface 7 in the sample-placing part 2, the distance between the contact position and the liquid surface 7 in the sample-placing part 2 is smaller than the preset height, and the position between the sample-adding part 5 and the liquid surface 7 in the sample-placing part 2 is in the second state, as shown in FIG. 6C, after the sample-adding part 5 discharges the sample to be added, the contact position on the sample-adding part 5 just contacts the liquid surface 7 in the sample-placing part 2, that is, the position between the sample-adding part 5 and the liquid surface 7 in the sample-placing part 2 is in the first state.
The sample contact position on the sample addition part may be any position between the front end part of the sample addition part and the sample discharge port of the sample addition part, the front end part is close to the sample discharge port, and specifically, may be the front end part, the sample discharge port, an intermediate position between the front end part and the sample discharge port, or the like.
The preset height can be the sample liquid drop height of a natural falling formed when the sample adding part performs sample discharging operation, the preset height can be known through a previous experiment, for example, the sample adding part is adopted for sample dropping operation, and the height value of the sample liquid drop of the natural falling formed is known through measurement, so that the sample liquid drop height value can be used as a numerical value of the preset height, and the preset height can be 0-1 mm, for example, 0mm, 0.5mm and 1mm in practical application. In both the first state and the second state, the sample liquid drop formed on the sample adding part can be contacted with the liquid surface in the sample placing part, so that viscous force is generated between the sample liquid drop and the liquid surface in the sample placing part, and simultaneously liquid surface tension in the sample placing part can also exert action on the sample liquid drop. And then along with the upward movement of application of sample portion, the combined action of liquid viscous force and surface tension in putting the appearance portion and sample liquid drop self gravity drops into and puts the appearance portion in, has avoided the emergence of sample wall built-up and the circumstances of remaining, and application of sample mode degree of accuracy is higher.
In one embodiment, before the step of approaching the sample addition part to the sample placement part, the method for mixing and adding samples may further include the steps of: and moving the sample adding part to the upper part of the sample placing part.
After the sample is sucked by the sample adding part, a position relation between the sample adding part and the sample placing part can be formed, specifically, the sample adding part can be moved to the upper part of the sample placing part, and certainly, the sample placing part can be directly moved to the lower part of the sample adding part when the operation of placing the sample placing part in the suspension position is executed, so that the step can be omitted.
In one embodiment, the step of bringing the sample addition part closer to the sample placement part and the step of placing the sample placement part in the suspended position may be performed simultaneously.
If the initial position of the sample addition part is such that it also needs to move to the vicinity of the suspended position, in order to save the time for transferring the component, the sample addition part can be moved synchronously while the operation of placing the sample placement part at the suspended position is performed, so that the sample addition part is moved to the position above the sample placement part placed at the suspended position.
In one embodiment, the step of placing the sample placement part in the suspended position may be: the transfer part moves the sample placing part from a placing position to a suspending position; the distance between the placing position and the detection position is larger than that between the suspension position and the detection position, so that the suspension position moves to the detection position, the moving path of the transfer part can be saved, and the moving time of the transfer part is reduced.
The placement position may be a placement area of the sample placement section, for example, a plurality of sample placement sections are placed in a sample placement section box located on the placement area, or a sample placement section is placed on the placement area, the placement area providing a support surface for the sample placement sections to bear the bottoms of the sample placement sections. In the conventional sample adding method, the sample placing part usually performs the sample adding operation in the placing area, and after the mixing is completed, the sample placing part is moved to the detection position, so that more time is consumed. As shown in fig. 6A, the sample placing part 2 can be moved from the placing position 1 to the suspending position 3, and then moved from the suspending position 3 to the detecting position 4. The sample placing unit may be placed and moved by a transfer unit. The sample placing part is clamped and grabbed by the transfer part and is driven to move, after the sample placing part reaches the corresponding position, if the position has no supporting surface, such as the suspended position, the transfer part holds and fixes the sample placing part to keep the sample placing part at the corresponding position for stopping, and if the position has the supporting surface, such as the detection position, the transfer part releases the sample placing part and places the sample placing part at the corresponding position.
As shown in FIG. 6D, when the sample application part 2 placed at the detection position 4 has completed the sample application and mixing operation, the detection operation of the substance to be applied such as a sample and/or a reagent can be performed.
In one embodiment, when the position at which the sample placement unit is in the kneading state is the suspended position, the step of moving the sample placement unit to the detection position by the transfer unit may include:
the transfer part transfers the sample placing part from the suspension position to the detection position;
the transfer unit releases the sample-placing unit and places the sample-placing unit at the detection position.
As shown in fig. 7, the transfer unit 6 is switched from a state of holding the fixed sample placement unit 2 to be located at the suspended position 3 to a state of moving the sample placement unit 2, moves from the suspended position 3 to the detection position 4, and releases the sample placement unit 2 after reaching the detection position 4, so that the movement process is smooth and stable.
In one embodiment, the step of the transfer part moving the sample placement part from the placement position to the suspension position may include:
the transfer part clamps the sample placing part positioned on the placing position;
the transfer part transfers the sample placing part to the suspension position.
As shown in fig. 8, the transfer unit 6 grips the specimen collection unit 2 and moves from the placement position 1 to the hanging position 3.
The invention also provides a sample analysis method, which comprises the sample adding and mixing method in any embodiment. The sample analysis method can be applied to a blood coagulation analyzer and is used for performing functional analysis on blood such as fibrinolysis, anti-fibrinolysis, blood coagulation, anticoagulation and the like. The sample can be a blood sample, a blood detection reagent, and the like, such as blood, blood components, fibrinolysis, anti-fibrinolysis, blood coagulation, anticoagulation, and the like detection reagents, and the blood components can be plasma, blood cells, and the like.
The invention also provides a sample-adding and mixing device, in an embodiment, as shown in fig. 9, the sample-adding and mixing device may include: the sample loading part, the first sample loading driving part, the second sample loading driving part, the transferring part and the control part; the sample adding part is used for absorbing an object to be added and adding a sample into the sample adding part; the sample adding part can be a sample needle; the first sample adding driving part is used for driving the movement of the sample adding part; the first sample adding driving part can be a power element such as an XYZ three-axis motion platform, a rotary motion platform, a motor and the like, and of course, can also be a transferring part shared with a sample placing part; the second sample adding driving part is used for driving the sample adding part to realize the suction or discharge operation of the object to be added; the second sample addition driving part can be an injector; the working principle of the injector can be that the plunger in the injector moves up and down under the driving of the driving motor, so that the volume change of the sealed cavity is caused to form different vacuum degrees, and the suction and the discharge of liquid are achieved. The second sample adding driving part is connected with the sample adding part and can be used for driving the sample adding part to realize the suction or discharge operation of a sample or a reagent. The transfer part is provided with a clamping mechanism for clamping and releasing the sample placing part and a blending mechanism for blending the sample placing part; the control part is used for controlling the first sample adding driving part, the second sample adding driving part and the transferring part; the control unit includes a processor and a memory, and the memory stores a computer program that, when executed by the processor, causes the processor to execute the sample-adding and mixing method according to any one of the embodiments. The control unit may drive the movement of the transfer unit by a transfer unit driving unit; the transfer unit driving unit may be an XYZ three-axis motion platform, an air cylinder, a motor, or the like.
The skilled person can understand that the sample adding and mixing method can be manufactured into a control chip or a storage chip and then applied to various types of analysis detection/analysis instruments, so that the application range and the application scene of the sample adding and mixing method are expanded, and the convenience of application of the sample adding and mixing method is improved.
In one embodiment, the sample-adding and mixing device may further include: and the sample placing part is used for placing the object to be added.
The invention also provides a computer storage medium, which stores a computer program, and when the computer program is executed by a processor, the processor executes the sample adding and mixing method according to any embodiment.
It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above may be implemented by a computer program, which may be stored in a non-volatile computer readable storage medium, and when executed, may include the processes of the embodiments of the methods described above. Any reference to memory, storage, database, or other medium used in the embodiments provided herein may include non-volatile and/or volatile memory, among others. Non-volatile memory can include read-only memory (ROM), Programmable ROM (PROM), Electrically Programmable ROM (EPROM), Electrically Erasable Programmable ROM (EEPROM), or flash memory. Volatile memory can include Random Access Memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms such as Static RAM (SRAM), Dynamic RAM (DRAM), Synchronous DRAM (SDRAM), Double Data Rate SDRAM (DDRSDRAM), Enhanced SDRAM (ESDRAM), Synchronous Link DRAM (SLDRAM), Rambus Direct RAM (RDRAM), direct bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).
The invention also provides a sample analysis device which comprises the sample adding and mixing device in any embodiment. The sample analysis device can be a blood coagulation analyzer and is used for performing functional analysis such as fibrinolysis, anti-fibrinolysis, blood coagulation and anticoagulation on blood. The sample can be a blood sample, a blood detection reagent, and the like, such as blood, blood components, fibrinolysis, anti-fibrinolysis, blood coagulation, anticoagulation, and the like detection reagents, and the blood components can be plasma, blood cells, and the like.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.

Claims (19)

1. The method for sample adding and uniform mixing is characterized by comprising the following steps:
placing the sample placing part on a suspension position;
the sample adding part adds a substance to be added to the sample placing part;
uniformly mixing the sample placing parts through a transferring part; under the action of the transfer part, the sample placing part has a moving state and a blending state;
after the step of adding the substance to be added to the sample placing part by the sample adding part, the method for uniformly adding and mixing the sample also comprises the following steps: the transfer part moves the sample placing part to a detection position; the detection position refers to a position where the sample placing part can be placed and an object to be detected in the sample placing part can be detected;
the transfer unit includes a member or structure having a clamping function;
the transfer part is a mechanical hand, a finger cylinder or a clamping jaw, and the transfer part can drive the sample placing part to be in a moving state, can also drive the sample placing part to be in a uniform mixing state, and further uniformly shakes the objects to be added in the sample placing part.
2. The method of claim 1, wherein the position of the sample placement part in the mixing state is the suspended position or any position that the sample placement part can pass through when moving from the suspended position to the detection position.
3. The method according to claim 1, wherein the sample placing unit is fixed by the transfer unit so that the sample placing unit is suspended when the sample placing unit is located at the suspended position.
4. The method for sample adding and mixing according to claim 3, further comprising the following steps:
detecting whether the sample placing part is placed on the transferring part;
acquiring the current position of the sample placement part when the sample placement part is placed on the transfer part;
and controlling the sample placing part to be in the state by the transfer part according to the current position of the sample placing part.
5. The method according to claim 3 or 4, wherein the sample-placing section is in one of the following states: the moving state, the blending state, the suspending state, the moving state and the blending state, and the blending state and the suspending state.
6. The method for sample adding and mixing according to claim 1,
the substance to be added is a sample and/or a reagent;
the step of adding the substance to be added to the sample placing part by the sample adding part comprises the following steps:
the sample adding part and the sample placing part are close to each other;
the sample adding part adds a substance to be added to the sample placing part at a sample adding position;
the sample adding part and the sample placing part are far away from each other.
7. The method according to claim 6, wherein the liquid surface in the sample-placing part is in a first state or a second state between the sample-placing part and the liquid surface after the sample-to-be-added substance is discharged to the sample-placing part; the first state is that the liquid contact position on the sample adding part just contacts the liquid surface in the sample placing part; and the second state is that the liquid contact position on the sample adding part is positioned above the liquid level in the sample placing part, and the distance between the liquid contact position and the liquid level in the sample placing part is less than the preset height.
8. The method according to claim 1 or 6, wherein the sample-adding part adds the substance to be added to the sample-placing part located in the suspended position.
9. The method for uniformly mixing and applying a sample according to claim 6, wherein the step of bringing the sample-applying part into proximity with the sample-placing part and the step of placing the sample-placing part in a suspended position are performed simultaneously.
10. The method for uniformly mixing and loading a sample according to claim 1, wherein the step of placing the sample placement member in a suspended position comprises: the transfer part moves the sample placing part from a placing position to a suspending position; the distance between the placing position and the detection position is larger than the distance between the suspending position and the detection position.
11. The method for uniformly mixing and loading a sample according to claim 1, wherein the step of moving the sample placement unit from the placement position to the suspended position by the transfer unit comprises:
the transfer part clamps the sample placing part positioned on the placing position;
the transfer part transfers the sample placing part to the suspension position.
12. The method for sample adding and uniform mixing according to claim 10, wherein the suspending position is located above the placing position, above the detecting position, or between the placing position and the detecting position; the placing position refers to a position where the sample placing part is placed before the sample placing part is placed at the suspension position.
13. The method according to claim 1, wherein the step of kneading the sample placement parts by the transfer part comprises: the transfer part applies driving force which can enable the sample placing parts to execute blending action to the sample placing parts clamped by the transfer part; the driving force includes at least one of a rotational force, a pendulum force, and a vibration force.
14. The method according to claim 1, wherein the step of moving the sample placement unit to the detection position by the transfer unit when the position where the sample placement unit is in the kneading state is the suspended position comprises:
the transfer part transfers the sample placing part from the suspension position to the detection position;
the transfer unit releases the sample-placing unit and places the sample-placing unit at the detection position.
15. A method of sample analysis, comprising: a method of loading and mixing according to any one of claims 1 to 14.
16. The utility model provides a sample application mixing device, its characterized in that, sample application mixing device includes:
the sample adding part is used for absorbing the object to be added and adding the sample into the sample placing part;
a first sample adding driving part for driving the movement of the sample adding part;
the second sample adding driving part is used for driving the sample adding part to realize the absorption or addition operation of the object to be added;
the transfer part is used for moving and uniformly mixing the sample placing part;
a control unit for controlling the first sample application driving unit, the second sample application driving unit, and the transfer unit;
the control unit includes a processor and a memory, and the memory stores a computer program that, when executed by the processor, causes the processor to execute the sample application and mixing method according to any one of claims 1 to 14.
17. The sample adding and mixing device of claim 16, further comprising: and the sample placing part is used for placing the object to be added.
18. A computer storage medium, in which a computer program is stored, which, when executed by a processor, causes the processor to execute the sample application and mixing method according to any one of claims 1 to 14.
19. A sample analyzer, comprising the sample application and mixing device according to claim 16 or 17.
CN201911206958.5A 2019-11-29 2019-11-29 Sample adding and mixing method, sample adding and mixing device, computer storage medium, sample analysis method and sample analysis device Active CN112871029B (en)

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