CN110921850A - Method for treating sewage by using microorganisms - Google Patents
Method for treating sewage by using microorganisms Download PDFInfo
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- CN110921850A CN110921850A CN202010083702.6A CN202010083702A CN110921850A CN 110921850 A CN110921850 A CN 110921850A CN 202010083702 A CN202010083702 A CN 202010083702A CN 110921850 A CN110921850 A CN 110921850A
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- 239000010865 sewage Substances 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 33
- 244000005700 microbiome Species 0.000 title claims abstract description 24
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 40
- 230000000813 microbial effect Effects 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 31
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract description 30
- 230000001580 bacterial effect Effects 0.000 claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 20
- 241001070941 Castanea Species 0.000 claims abstract description 19
- 235000014036 Castanea Nutrition 0.000 claims abstract description 19
- 239000003463 adsorbent Substances 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 15
- 239000001888 Peptone Substances 0.000 claims abstract description 12
- 108010080698 Peptones Proteins 0.000 claims abstract description 12
- 240000000111 Saccharum officinarum Species 0.000 claims abstract description 12
- 235000007201 Saccharum officinarum Nutrition 0.000 claims abstract description 12
- 235000013379 molasses Nutrition 0.000 claims abstract description 12
- 235000019319 peptone Nutrition 0.000 claims abstract description 12
- 239000002131 composite material Substances 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229920001661 Chitosan Polymers 0.000 claims abstract description 7
- 230000004913 activation Effects 0.000 claims abstract description 7
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 7
- 239000000661 sodium alginate Substances 0.000 claims abstract description 7
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 7
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 6
- 241000187809 Frankia Species 0.000 claims abstract description 6
- 241000191938 Micrococcus luteus Species 0.000 claims abstract description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 6
- 230000001590 oxidative effect Effects 0.000 claims abstract description 6
- 238000010000 carbonizing Methods 0.000 claims abstract description 5
- 241001122767 Theaceae Species 0.000 claims description 15
- 241000534460 Ampullaria Species 0.000 claims description 11
- 240000001548 Camellia japonica Species 0.000 claims description 8
- 235000018597 common camellia Nutrition 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000000839 emulsion Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 9
- 150000002500 ions Chemical class 0.000 abstract description 9
- 239000004816 latex Substances 0.000 abstract description 4
- 229920000126 latex Polymers 0.000 abstract description 4
- 244000269722 Thea sinensis Species 0.000 abstract 2
- 208000006758 Marek Disease Diseases 0.000 abstract 1
- 241000570011 Pomacea canaliculata Species 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 9
- 239000003344 environmental pollutant Substances 0.000 description 8
- 231100000719 pollutant Toxicity 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 description 2
- 229910001431 copper ion Inorganic materials 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- QALQXPDXOWOWLD-UHFFFAOYSA-N [N][N+]([O-])=O Chemical compound [N][N+]([O-])=O QALQXPDXOWOWLD-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/20—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbon; comprising carbon obtained by carbonising processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Biological Treatment Of Waste Water (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for treating sewage by using microorganisms, which comprises the steps of crushing chestnut shells and oil tea fruit shells, adding phosphoric acid for activation, and carbonizing to obtain an adsorbent; respectively crushing Pomacea canaliculata, oil tea cakes and sugarcane filter mud, adding into a stirring tank, and adding molasses, peptone and water to prepare a microbial nitrogen source; mixing micrococcus luteus, Frankia, nitrite oxidizing bacteria, Marek's disease bacteria, bacillus subtilis and EM bacteria to obtain a composite bacterial liquid; uniformly mixing chitosan, sodium alginate, talcum powder and redispersible latex powder to prepare a biological carrier; uniformly mixing the compound bacterial liquid and the microbial nitrogen source, then culturing, adding the adsorbent and the biological carrier, uniformly stirring, and drying to obtain the microbial sewage treatment agent. The microbial treatment agent prepared by the method can effectively remove ammonia nitrogen, heavy metal ions, COD and the like in the sewage, has low input cost and has good application prospect in sewage treatment.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a method for treating sewage by using microorganisms.
Background
With the advance of urbanization and the rapid development of industry, the environmental pollution problem becomes more and more serious. A large amount of domestic and industrial sewage flows into rivers, lakes or underground water, which causes serious pollution to water bodies and has negative effects on the health and safety of residents. The industrial sewage contains many pollutants and complex components, is not easy to purify in water, is difficult to treat, and is a problem which is urgently needed to be solved in the current environmental pollution problem.
The pollutants in the industrial sewage mainly comprise ammonia nitrogen, phosphorus, sulfide, inorganic salt and other substances, the ammonia nitrogen is an important pollutant causing water eutrophication and polluting the environment, and the ammonia nitrogen is one of the main pollutants in almost all polluted water areas in China at present. The eutrophication of water body has already endangered agriculture, fishery, tourism and the like
Many industries also pose a great threat to drinking water hygiene and food safety. Therefore, how to remove ammonia nitrogen in industrial sewage becomes an important task in industrial sewage treatment at present.
At present, the sewage treatment technology mainly comprises the following steps: (1) adding chemical substances into the sewage to degrade or recover pollutants in the sewage, wherein the chemical substances comprise a precipitation method, a neutralization method, an oxidation-reduction method, a chemical physical disinfection method and the like; (2) removing pollutants in the sewage by a physical and chemical method, wherein the method comprises an ion exchange method, an adsorption method, a membrane separation method and the like; (3) separating and recovering insoluble pollutants in the sewage by a physical method; (4) a method for degrading organic pollutants in sewage into stable and harmless substances by the metabolism of microorganisms. The method for treating sewage by microorganisms mainly comprises the steps of adding aerobic autotrophic microorganisms to convert organic nitrogen in industrial sewage into ammonia nitrogen, and finally degrading amino nitrogen and nitro nitrogen in a water body through the nitrification of nitrifying bacteria and the denitrification of denitrifying bacteria, so that nitrogen is released to escape from the water body, thereby completing the biogeochemical circulation process of nitrogen in the water body and reducing the concentration of ammonia nitrogen in the water body. There are certain disadvantages to this approach, such as: higher cost, low ammonia nitrogen removal efficiency and the like, and hinders the large-scale popularization of the microbial inoculum in industrial sewage.
Disclosure of Invention
The invention provides a method for treating sewage by using microorganisms, aiming at the problems of the existing microorganism sewage treatment. The method can effectively remove ammonia nitrogen, heavy metal ions, industrial COD and the like in the sewage by selecting a proper nitrogen source and combining reasonable compatibility of various strains and selecting an adsorbent with large specific surface area and an excellent biological carrier to prepare the microbial treatment agent, and has the advantages of low investment cost and easy realization of industrial production.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
1. a method for treating sewage by using microorganisms is characterized by comprising the following steps: the method comprises the following steps:
(1) crushing the chestnut shells and the oil tea fruit shells, preparing phosphoric acid into a solution with the mass concentration of 5-10% to soak the chestnut shells and the oil tea fruit shells for 1-3h for activation, and carbonizing at the temperature of 800-; the adding amount of the phosphoric acid is 1-3% of the total mass of the chestnut shells and the oil tea shells; the mass ratio of the chestnut shells to the oil-tea camellia shells is 1-3: 1;
(2) respectively crushing the ampullaria gigas, the oil-tea cake and the sugarcane filter mud, adding the crushed materials into a stirring tank, and adding molasses, peptone and water; after being stirred evenly, the mixture is kept for 30 to 60min at the temperature of 100-; the mass ratio of the ampullaria gigas to the oil-tea camellia cake to the sugarcane filter mud to the molasses to the peptone to the water is 1:2:5:0.5:1: 10;
(3) respectively culturing micrococcus luteus, Frankia, nitrite oxidizing bacteria, Marek's bacteria, bacillus subtilis and EM bacteria in a mass ratio of 3:2:5:1:5:8 into bacterial liquids with the concentration of 1 x 107-1 x 108 bacteria/ml, and mixing to obtain a composite bacterial liquid;
(4) uniformly mixing chitosan, sodium alginate, talcum powder and redispersible emulsion powder in a mass ratio of 1:5:10:3 to prepare the biological carrier;
(5) uniformly mixing the compound bacterial liquid and a microbial nitrogen source, culturing for 24-48h, adding an adsorbent and a biological carrier, uniformly stirring, and drying to obtain a microbial sewage treatment agent; the mass ratio of the composite bacterial liquid to the microbial nitrogen source to the adsorbent to the biological carrier is 1:3:10: 5;
(6) after the sewage is subjected to solid-liquid separation, the ammonia nitrogen content is firstly detected, and then a microbial sewage treatment agent is added to complete the sewage treatment.
Further, the incubation in the step (5) is performed at a temperature of 25-28 ℃ and a rotation speed of 100-.
Furthermore, the addition amount of the microbial sewage treatment agent is 5-50g per liter of sewage.
Compared with the prior art, the invention has the advantages and beneficial effects that:
1. the method provided by the invention has the advantages that the proper nitrogen source is selected, the reasonable compatibility of various strains is combined, the symbiotic coordination is realized, the activity is high, the propagation speed is high, the adsorbent with large specific surface area and the excellent biological carrier are selected, the prepared microbial treatment agent can effectively remove ammonia nitrogen, heavy metal ions, COD (chemical oxygen demand) and the like in the sewage, the investment cost is low, the industrial production is easy to realize, and the method has a good application prospect in the sewage treatment.
2. The adsorbent prepared by carbonizing chestnut shells and oil-tea camellia fruits after phosphoric acid activation has a large specific surface area, improves the contact area of strains and pollutants, improves the adsorption efficiency, and can adsorb heavy metal ions in sewage.
3. According to the method, the ampullaria gigas, the oil-tea cake, the sugarcane filter mud, the molasses and the peptone are used as biological nitrogen sources, so that nutrients required for the growth of microbial strains can be obtained, the propagation of the strains can be rapidly promoted, and the problem that the growth of microorganisms is not suitable due to high-pollution industrial sewage can be solved.
4. According to the invention, chitosan, sodium alginate, talcum powder and redispersible emulsion powder are used as biological carriers, so that a microbial agent can be well fixed, nutrient substances can be more easily absorbed by thalli, and the sewage purification effect of microorganisms is greatly improved.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments. It should be emphasized that the following description is merely exemplary in nature and is not intended to limit the scope of the invention or its application.
Example 1
A method for treating sewage by using microorganisms comprises the following steps:
(1) after the chestnut shells and the oil tea fruit shells are crushed according to the mass ratio of 2:1, phosphoric acid is prepared into a solution with the mass concentration of 5-10% to soak the chestnut shells and the oil tea fruit shells for activation for 2 hours, the addition amount of the phosphoric acid is 2% of the total amount of the chestnut shells and the oil tea fruit shells, and the phosphoric acid is carbonized for 24 hours at the temperature of 850 ℃ to obtain the adsorbent.
(2) Respectively crushing the ampullaria gigas, the oil-tea cake and the sugarcane filter mud, adding the crushed materials into a stirring tank, and adding molasses, peptone and water; stirring, keeping the temperature at 100 deg.C for 60min, and cooling to obtain microorganism nitrogen source; the mass ratio of the ampullaria gigas to the oil-tea camellia cake to the sugarcane filter mud to the molasses to the peptone to the water is 1:2:5:0.5:1: 10.
(3) Respectively culturing Micrococcus luteus, Frankia, nitrite oxidizing bacteria, Marek's bacteria, Bacillus subtilis and EM bacteria at the mass ratio of 3:2:5:1:5:8 to obtain culture liquid with the concentration of 1 × 108And mixing the bacterial liquid of each/ml to obtain the composite bacterial liquid.
(4) Mixing chitosan, sodium alginate, talcum powder and redispersible latex powder according to the mass ratio of 1:5:10:3 to prepare the biological carrier.
(5) Uniformly mixing the compound bacterial liquid and a microbial nitrogen source, culturing at the temperature of 25 ℃ and the rotating speed of 120r/min, adding an adsorbent and a biological carrier, uniformly stirring, and drying to obtain a microbial sewage treatment agent; the mass ratio of the composite bacterial liquid to the microbial nitrogen source to the adsorbent to the biological carrier is 1:3:10: 5.
(6) After solid-liquid separation is carried out on the chemical plant sewage, the detection of the contents of ammonia nitrogen, heavy metal ions and COD is carried out, a microbial sewage treatment agent is added according to the dosage of 50g per liter of sewage, and the sewage treatment can be completed according to the conventional sewage treatment process.
Example 2
A method for treating sewage by using microorganisms comprises the following steps:
(1) after the chestnut shells and the oil tea fruit shells are crushed according to the mass ratio of 3:1, phosphoric acid is prepared into a solution with the mass concentration of 5-10% to soak the chestnut shells and the oil tea fruit shells for activation for 1h, the addition amount of the phosphoric acid is 3% of the total amount of the chestnut shells and the oil tea fruit shells, and the adsorbent is obtained by carbonizing the phosphoric acid at 900 ℃ for 20 h.
(2) Respectively crushing the ampullaria gigas, the oil-tea cake and the sugarcane filter mud, adding the crushed materials into a stirring tank, and adding molasses, peptone and water; stirring, keeping the temperature at 110 ℃ for 40min, and cooling to obtain a microbial nitrogen source; the mass ratio of the ampullaria gigas to the oil-tea camellia cake to the sugarcane filter mud to the molasses to the peptone to the water is 1:2:5:0.5:1: 10.
(3) Respectively culturing Micrococcus luteus, Frankia, nitrite oxidizing bacteria, Marek's bacteria, Bacillus subtilis and EM bacteria at the mass ratio of 3:2:5:1:5:8 to obtain culture liquid with the concentration of 1 × 107And mixing the bacterial liquid of each/ml to obtain the composite bacterial liquid.
(4) Mixing chitosan, sodium alginate, talcum powder and redispersible latex powder according to the mass ratio of 1:5:10:3 to prepare the biological carrier.
(5) Uniformly mixing the compound bacterial liquid and a microorganism nitrogen source, culturing at the temperature of 28 ℃ and the rotating speed of 150r/min, adding an adsorbent and a biological carrier, uniformly stirring, and drying to obtain a microorganism sewage treatment agent; the mass ratio of the composite bacterial liquid to the microbial nitrogen source to the adsorbent to the biological carrier is 1:3:10: 5.
(6) After solid-liquid separation is carried out on the textile mill sewage, the detection of the content of ammonia nitrogen, heavy metal ions and COD is carried out, a microbial sewage treatment agent is added according to the dosage of 30g per liter of sewage, and the sewage treatment can be completed according to the conventional sewage treatment process.
Example 3
A method for treating sewage by using microorganisms comprises the following steps:
(1) after the chestnut shells and the oil tea fruit shells are crushed according to the mass ratio of 1:1, phosphoric acid is prepared into a solution with the mass concentration of 5-10% to soak the chestnut shells and the oil tea fruit shells for activation for 1h, the addition amount of the phosphoric acid is 3% of the total amount of the chestnut shells and the oil tea fruit shells, and the phosphoric acid is carbonized for 24h at the temperature of 800 ℃ to obtain the adsorbent.
(2) Respectively crushing the ampullaria gigas, the oil-tea cake and the sugarcane filter mud, adding the crushed materials into a stirring tank, and adding molasses, peptone and water; stirring, keeping the temperature at 120 deg.C for 30min, and cooling to obtain microorganism nitrogen source; the mass ratio of the ampullaria gigas to the oil-tea camellia cake to the sugarcane filter mud to the molasses to the peptone to the water is 1:2:5:0.5:1: 10.
(3) Respectively culturing Micrococcus luteus, Frankia, nitrite oxidizing bacteria, Marek's bacteria, Bacillus subtilis and EM bacteria at the mass ratio of 3:2:5:1:5:8 to obtain culture liquid with the concentration of 1 × 108And mixing the bacterial liquid of each/ml to obtain the composite bacterial liquid.
(4) Mixing chitosan, sodium alginate, talcum powder and redispersible latex powder according to the mass ratio of 1:5:10:3 to prepare the biological carrier.
(5) Uniformly mixing the compound bacterial liquid and a microorganism nitrogen source, culturing at the temperature of 28 ℃ and the rotating speed of 100r/min, adding an adsorbent and a biological carrier, uniformly stirring, and drying to obtain a microorganism sewage treatment agent; the mass ratio of the composite bacterial liquid to the microbial nitrogen source to the adsorbent to the biological carrier is 1:3:10: 5.
(6) After solid-liquid separation is carried out on the sewage of the metallurgical plant, the detection of the contents of ammonia nitrogen, heavy metal ions and COD is carried out, a microbial sewage treatment agent is added according to the dosage of 15g per liter of sewage, and the sewage treatment can be completed according to the conventional sewage treatment process. .
The results of the treatment using the microbial wastewater treatment agent of the present invention for wastewater treatment are shown in tables 1 and 2.
Table 1: the microbial sewage treatment agent has the effect of removing ammonia nitrogen and CODcr
| Product(s) | Ammonia nitrogen content mg/L before treatment | The ammonia nitrogen content mg/L after treatment | Ammonia nitrogen removal (%) | COD content mg/L before treatment | COD content mg/L after treatment | COD removal Rate (%) |
| Example 1 | 619.27 | 4.21 | 99.32 | 817.63 | 8.29 | 98.98 |
| Example 2 | 318.39 | 4.14 | 98.69 | 421.71 | 7.13 | 98.31 |
| Example 3 | 81.32 | 2.98 | 96.33 | 102.69 | 3.05 | 97.03 |
From the test results, the microbial sewage treatment agent disclosed by the invention has the effect of removing ammonia nitrogen and CODcr, and the treated sewage meets the national industrial water discharge standard.
Table 2: the microbial sewage treatment agent has the effect of removing heavy metal ions
| Product(s) | Copper ion content mg/L before treatment | The copper ion content after treatment is mg/L | Cadmium ion content mg/L before treatment | The content of cadmium ions after treatment is mg/L | Lead ion content mg/L before treatment | The lead ion content after treatment is mg/L |
| Example 1 | 2.30 | 0.028 | 2.68 | 0.021 | 3.12 | 0.018 |
| Example 2 | 1.92 | 0.016 | 1.01 | 0.013 | 2.16 | 0.024 |
| Example 3 | 5.63 | 0.051 | 3.69 | 0.038 | 1.98 | 0.038 |
From the test results, the microbial sewage treatment agent prepared by the invention has a good effect of removing heavy metal ions.
The foregoing is a more detailed description of the invention in connection with specific/preferred embodiments and is not intended to limit the practice of the invention to those descriptions. It will be apparent to those skilled in the art that various substitutions and modifications can be made to the described embodiments without departing from the spirit of the invention, and such substitutions and modifications are to be considered as within the scope of the invention.
Claims (3)
1. A method for treating sewage by using microorganisms is characterized by comprising the following steps: the method comprises the following steps:
(1) crushing the chestnut shells and the oil tea fruit shells, preparing phosphoric acid into a solution with the mass concentration of 5-10% to soak the chestnut shells and the oil tea fruit shells for 1-3h for activation, and carbonizing at the temperature of 800-; the adding amount of the phosphoric acid is 1-3% of the total mass of the chestnut shells and the oil tea shells; the mass ratio of the chestnut shells to the oil-tea camellia shells is 1-3: 1;
(2) respectively crushing the ampullaria gigas, the oil-tea cake and the sugarcane filter mud, adding the crushed materials into a stirring tank, and adding molasses, peptone and water; after being stirred evenly, the mixture is kept for 30 to 60min at the temperature of 100-; the mass ratio of the ampullaria gigas to the oil-tea camellia cake to the sugarcane filter mud to the molasses to the peptone to the water is 1:2:5:0.5:1: 10;
(3) respectively culturing Micrococcus luteus, Frankia, nitrite oxidizing bacteria, Marek's bacteria, Bacillus subtilis and EM bacteria at the mass ratio of 3:2:5:1:5:8 to obtain culture liquid with the concentration of 1 × 107-1×108Mixing the bacterial liquid of each ml to obtain a composite bacterial liquid;
(4) uniformly mixing chitosan, sodium alginate, talcum powder and redispersible emulsion powder in a mass ratio of 1:5:10:3 to prepare the biological carrier;
(5) uniformly mixing the compound bacterial liquid and a microbial nitrogen source, culturing for 24-48h, adding an adsorbent and a biological carrier, uniformly stirring, and drying to obtain a microbial sewage treatment agent; the mass ratio of the composite bacterial liquid to the microbial nitrogen source to the adsorbent to the biological carrier is 1:3:10: 5;
(6) after the sewage is subjected to solid-liquid separation, the ammonia nitrogen content is firstly detected, and then a microbial sewage treatment agent is added to complete the sewage treatment.
2. The method for treating sewage using microorganisms according to claim 1, wherein: the cultivation in the step (5) is carried out at a temperature of 25-28 ℃ and a rotation speed of 100-150 r/min.
3. The method for treating sewage using microorganisms according to claim 1, wherein: the addition amount of the microbial sewage treatment agent is 5-50g per liter of sewage.
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