CN104528955A - Microbial preparation for restoring fertilizer plant sewage and application thereof - Google Patents
Microbial preparation for restoring fertilizer plant sewage and application thereof Download PDFInfo
- Publication number
- CN104528955A CN104528955A CN201510038477.3A CN201510038477A CN104528955A CN 104528955 A CN104528955 A CN 104528955A CN 201510038477 A CN201510038477 A CN 201510038477A CN 104528955 A CN104528955 A CN 104528955A
- Authority
- CN
- China
- Prior art keywords
- microbial preparation
- prepare
- atcc
- sewage
- microbe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 43
- 239000010865 sewage Substances 0.000 title claims abstract description 41
- 230000000813 microbial effect Effects 0.000 title claims abstract description 40
- 239000003337 fertilizer Substances 0.000 title claims abstract description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 17
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 241000196324 Embryophyta Species 0.000 claims description 17
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 17
- 241000209094 Oryza Species 0.000 claims description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 239000012467 final product Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 239000010902 straw Substances 0.000 claims description 16
- 239000002594 sorbent Substances 0.000 claims description 15
- 241000194032 Enterococcus faecalis Species 0.000 claims description 13
- 241000191938 Micrococcus luteus Species 0.000 claims description 13
- 241000187563 Rhodococcus ruber Species 0.000 claims description 13
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 13
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 12
- 244000105624 Arachis hypogaea Species 0.000 claims description 12
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 12
- 235000018262 Arachis monticola Nutrition 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 235000012054 meals Nutrition 0.000 claims description 12
- 235000020232 peanut Nutrition 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 241000589291 Acinetobacter Species 0.000 claims description 10
- 241000186063 Arthrobacter Species 0.000 claims description 10
- 229910021536 Zeolite Inorganic materials 0.000 claims description 9
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 9
- 239000004021 humic acid Substances 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 239000000377 silicon dioxide Substances 0.000 claims description 9
- 229960001866 silicon dioxide Drugs 0.000 claims description 9
- 235000012239 silicon dioxide Nutrition 0.000 claims description 9
- 239000010457 zeolite Substances 0.000 claims description 9
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 8
- 229920001661 Chitosan Polymers 0.000 claims description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 8
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 241000588626 Acinetobacter baumannii Species 0.000 claims description 3
- 241001524201 Arthrobacter crystallopoietes Species 0.000 claims description 3
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 2
- 150000002989 phenols Chemical class 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000013049 sediment Substances 0.000 abstract 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 238000005842 biochemical reaction Methods 0.000 description 4
- 238000012851 eutrophication Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000004576 sand Substances 0.000 description 4
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 3
- 150000001299 aldehydes Chemical class 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000001311 chemical methods and process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- JYYOBHFYCIDXHH-UHFFFAOYSA-N carbonic acid;hydrate Chemical compound O.OC(O)=O JYYOBHFYCIDXHH-UHFFFAOYSA-N 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to the technical field of microbes, and discloses a microbial preparation for restoring fertilizer plant sewage. The microbial preparation for restoring fertilizer plant sewage is prepared by the following steps: 1) preparing an adsorbent; 2) preparing a microbial carbon source; 3) preparing a microbial carrier; and 4) preparing the microbial preparation. The microbial preparation can effectively remove ammonia nitrogen, SS (suspended sediment) and phenols in the fertilizer plant sewage, has favorable removal effect, and achieves the discharge standard.
Description
Technical field
The invention belongs to microbial technology field, disclosing a kind of microbial preparation for repairing fertilizer plant's sewage and application thereof.
Background technology
Fertilizer plant's sewage is the important sources of industrial sewage, and the pollutent index that mainly exceeds standard is ammonia nitrogen, sulfide and aldehydes matter.In sewage, all kinds of pollutant load is high, belongs to high pollution thing sewage, ammonia nitrogen concentration Gao Keda 700mg/L, and COD can reach more than 1000mg/L, and sulfide can reach 100mg/L, and phenols can reach 50mg/L.At present, physico-chemical process, biochemical integrated process, novel biological dinitrification method and traditional biological denitrogenation are divided into the method for high density pollution thing sewage disposal both at home and abroad.At present, more physico-chemical process, novel biological dinitrification method and traditional biological denitrogenation is studied.
Ammonia nitrogen causes the important pollution substance of the one of body eutrophication and contaminate environment, and in the Polluted water area that current China is nearly all, ammonia nitrogen is all one of main pollutent.Body eutrophication has endangered all conglomeraties such as agricultural, fishery, tourism, also constitutes huge threat to drinking water hygiene and food safety.Water pollution particularly body eutrophication has become the important influence factor of China's Economic development.Normal containing ammonia nitrogen in high density in chemical fertilizer industry sewage, be one of ammonia nitrogen in sewage main source.Therefore, the ammonia nitrogen how removed in chemical fertilizer industry sewage has become a major issue in current Industrial Waste Water Treatments.Ammonia nitrogen is the principal pollutant of fertilizer plant's sewage, and entering water body can cause body eutrophication, causes water quality deterioration, makes discharge be subject to strict restriction.Fertilizer plant's sewage is mainly from the high concentration ammonia nitrogen sewage of synthetic ammonia, urea plant, and the main existence form of this part sewage ammonia nitrogen is inorganic ammonia.In sum, along with expanding economy, the environmental stress that fertilizer plant faces is increasingly strict, and in order to the needs of sustained economic development, in actual production, sewage effluent becomes giving top priority to what is the most important of fertilizer plant's environmental problem.
For a long time, adding of biochemical sewage process carbon source uses methyl alcohol, white sugar (or cerelose) always, and methyl alcohol belongs to harmful influence, in use not only there is very large danger; White sugar cost is higher, and enterprise is generally beyond affordability.The mechanism of action of microbial preparation in environment protection is mainly: pollutant oxidation to be decomposed into the end product such as carbonic acid gas and water by metabolic reaction or to be converted into the nutritive substance of microorganism by microbial inoculum; promote the growth and breeding of self, just as H2S can be changed into the element sulphur required for own growth by photosynthetic bacterium and genus bacillus etc.It is unreasonable mostly to there is bacteria microorganism compatibility in the microbial preparation of prior art process fertilizer plant sewage, and removal efficiency is low, and and cost is higher, and enterprise is difficult to the defects such as acceptance, greatly hinders the application process of Biochemical method sewage.
Summary of the invention
The technical issues that need to address of the present invention are, overcome the deficiencies in the prior art part, provide a kind of microbial preparation for repairing fertilizer plant's sewage and application thereof, this microbial preparation comprises multiple bacterial strain, compatibility is reasonable, and synergy is strong, effectively can remove the ammonia nitrogen in sewage, sulfide, aldehydes matter and industrial COD, low in input cost, can large-scale promotion use.
The object of the invention is to be achieved through the following technical solutions:
For repairing a microbial preparation for fertilizer plant's sewage, it is prepared as follows and forms: step 1) prepare sorbent material, step 2) prepare microbe carbon source, step 3) prepare microbe carrier, step 4) prepare microbial preparation.
Particularly, described microbial preparation is prepared as follows and forms:
Step 1) prepare sorbent material: first zeolite is carried out pulverization process, obtain the zeolite powder of particle diameter between 50-100 order, then mix according to the weight ratio of 1: 2: 2 with silicon-dioxide, humic acids, to obtain final product;
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned rice straw powder and peanut hull meal are added in stirred pot, then dregs of beans and water is added, after the speed of 500 revs/min stirs 15 minutes, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30 minutes, last naturally cooling and get final product; The mass ratio of rice straw powder, peanut hull meal, dregs of beans and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100-200 order, then by chitosan, trehalose, diatomite and glycerine according to 1: 1: 1: 2 mass ratio mix, to obtain final product;
Step 4) prepare microbial preparation: it is 1 × 10 that micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, the brilliant Arthrobacter of one-tenth, many glutinous series bacillus and Pseudomonas fluorescens are trained concentration respectively
8the bacterium liquid of individual/ml, according to the volume ratio mixing of 7: 5: 5: 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours for 30 DEG C, add step 1 subsequently) sorbent material prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cryodrying, drying temperature is 20-22 DEG C, and after dry, water content is 10-12%, to obtain final product; The mass ratio of described sorbent material, microbe carrier carrier and microbe carbon source is 1: 2: 5.
Preferably,
Described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442;
Described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) is ATCC 29212;
Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906;
Described Acinetobacter bauamnnii is Acinetobacter bauamnnii (Acinetobacter baumanii) is ATCC 19606;
The brilliant Arthrobacter of described one-tenth is for becoming brilliant Arthrobacter (Arthrobacter crystallopoietes) ATCC 15481;
Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842;
Described Pseudomonas fluorescens is Pseudomonas fluorescens (P.Fluorescens) ATCC 49642.
The particle diameter of described silicon-dioxide or humic acids is 50-100 order.
The using method of mentioned microorganism preparation comprises: first remove the suspended substance in sewage, then enter settling tank, the fine sand of 50cm thickness laid by settling tank, precipitates 12 hours, enter biochemical reaction tank subsequently, the pH adding hydrochloric acid adjustment sewage is 7-7.5, adds 20 grams of microbial preparations at every turn, add 1 every day according to every cubic metre of liquid, after adding three days continuously, leave standstill one week again, liquid filtering is degerming, finally discharge.
Bacterial classification of the present invention all can be bought from commercial channel such as American Type Culture collection warehousings (ATCC) and obtain.The enlarged culturing method of bacterial strain of the present invention can record according to document or the record of prior art is carried out, and is not innovative point of the present invention herein, as space is limited, does not repeat one by one.
The beneficial effect that the present invention obtains is main but be not limited to the following aspects:
Reasonable compatibility between each bacterial classification in microbial preparation of the present invention, symbiosis is coordinated, mutually not antagonism, and active high, biomass is large, and breeding is fast; Microbial preparation of the present invention, by the various bacterial classification that can form dominant microflora, is mixed with high-effect bacterial, is added in Waste Water Treatment by a certain amount of, accelerates bacterial strain to the degraded of pollutent, to improve the biological treatment efficiency of system, ensures system stable operation; Microbial preparation of the present invention is suitable for fertilizer plant's sewage disposal, can improve the process water yield and water quality treatment, reduces working cost, promotes qualified discharge; The present invention has prepared microbe carbon source nutriment by agricultural wastes, and low price is easily accepted by enterprise; The present invention with the addition of the sorbent material that the silicon-dioxide of certain particle diameter, zeolite powder and humic acids are formed, and can be used to assemble pollutent and thalline, improves the contact area of bacterial strain and pollutent, improves bio-oxidation efficiency.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, the technical scheme of the application is clearly and completely described, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
For repairing a microbial preparation for fertilizer plant's sewage, it is prepared as follows and forms:
Step 1) prepare sorbent material: first zeolite is carried out pulverization process, obtain particle diameter at the zeolite powder of 50, then mix according to the weight ratio of 1: 2: 2 with silicon-dioxide, humic acids, to obtain final product; The particle diameter of described silicon-dioxide or humic acids is 50 orders.
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned rice straw powder and peanut hull meal are added in stirred pot, then dregs of beans and water is added, after the speed of 500 revs/min stirs 15 minutes, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor at 95 DEG C, insulation reaction 30 minutes, last naturally cooling and get final product; The mass ratio of rice straw powder, peanut hull meal, dregs of beans and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100 orders, then by chitosan, trehalose, diatomite and glycerine according to 1: 1: 1: 2 mass ratio mix, to obtain final product;
Step 4) prepare microbial preparation: it is 1 × 10 that micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, the brilliant Arthrobacter of one-tenth, many glutinous series bacillus and Pseudomonas fluorescens are trained concentration respectively
8the bacterium liquid of individual/ml, according to the volume ratio mixing of 7: 5: 5: 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours for 30 DEG C, add step 1 subsequently) sorbent material prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cryodrying, drying temperature is 20 DEG C, and after dry, water content is 10%, to obtain final product; The mass ratio of described sorbent material, microbe carrier carrier and microbe carbon source is 1: 2: 5.
The using method of mentioned microorganism preparation comprises: first remove the suspended substance in sewage, then enter settling tank, the fine sand of 50cm thickness laid by settling tank, precipitates 12 hours, enter biochemical reaction tank subsequently, the pH adding hydrochloric acid adjustment sewage is 7, adds 20 grams of microbial preparations at every turn, add 1 every day according to every cubic metre of liquid, after adding three days continuously, leave standstill one week again, liquid filtering is degerming, finally discharge.
Embodiment 2
For repairing a microbial preparation for fertilizer plant's sewage, it is prepared as follows and forms:
Step 1) prepare sorbent material: first zeolite is carried out pulverization process, obtain particle diameter at 100 object zeolite powders, then mix according to the weight ratio of 1: 2: 2 with silicon-dioxide, humic acids, to obtain final product; The particle diameter of described silicon-dioxide or humic acids is 100 orders;
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned rice straw powder and peanut hull meal are added in stirred pot, then dregs of beans and water is added, after the speed of 500 revs/min stirs 15 minutes, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor at 100 DEG C, insulation reaction 30 minutes, last naturally cooling and get final product; The mass ratio of rice straw powder, peanut hull meal, dregs of beans and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 200 orders, then by chitosan, trehalose, diatomite and glycerine according to 1: 1: 1: 2 mass ratio mix, to obtain final product;
Step 4) prepare microbial preparation: it is 1 × 10 that micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, the brilliant Arthrobacter of one-tenth, many glutinous series bacillus and Pseudomonas fluorescens are trained concentration respectively
8the bacterium liquid of individual/ml, according to the volume ratio mixing of 7: 5: 5: 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours for 30 DEG C, add step 1 subsequently) sorbent material prepared and step) microbe carriers of 3 preparations, 100 turns/min stirs 3min, finally carry out cryodrying, drying temperature is 22 DEG C, and after dry, water content is 12%, to obtain final product; The mass ratio of described sorbent material, microbe carrier carrier and microbe carbon source is 1: 2: 5.
Described micrococcus luteus be micrococcus luteus (Micrococcus luteus) ATCC 49442 (APPLIED ANDENVIRONMENTAL MICROBIOLOGY1992, p.3423-3425);
Described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) is ATCC 29212 (In Vivo.2009Jan-Feb; 23 (1): 81-7.);
Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906 (J.Bacteriol.November2001);
Described Acinetobacter bauamnnii is Acinetobacter bauamnnii (Acinetobacter baumanii) is ATCC 19606 (Infect Immun.2012Mar; 80 (3): 1015-24);
The brilliant Arthrobacter of described one-tenth is finished product Arthrobacter (Arthrobacter crystallopoietes) ATCC 15481 (Appl.Environ.Microbiol.March 15,200975:61774-1777).
Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842 (J.Microbiol.Biotechnol. (2006), 16 (10), 1650-1655);
Described Pseudomonas fluorescens is Pseudomonas fluorescens (P.Fluorescens) ATCC49642 (WO2003066873A1).
The using method of mentioned microorganism preparation comprises: first remove the suspended substance in sewage, then enter settling tank, the fine sand of 50cm thickness laid by settling tank, precipitates 12 hours, enter biochemical reaction tank subsequently, the pH adding hydrochloric acid adjustment sewage is 7, adds 20 grams of microbial preparations at every turn, add 1 every day according to every cubic metre of liquid, after adding three days continuously, leave standstill one week again, liquid filtering is degerming, finally discharge.
Embodiment 3
With the sewage of this city fertilizer plant for example, first remove the suspended substance in sewage, then enter settling tank, the fine sand of 50cm thickness laid by settling tank, precipitate 12 hours, enter biochemical reaction tank subsequently, the pH adding hydrochloric acid adjustment sewage is 7, add 20 grams of microbial preparations according to every cubic metre of liquid at every turn, add 1 every day, after adding three days continuously, then leave standstill one week, liquid filtering is degerming, finally discharge.Three water samples are all got in each detection, average; Each index all reaches desirable emission standard after testing, and the Testing index of sewage is in table 1:
Table 1
Conclusion: microbial preparation of the present invention can remove ammonia nitrogen, SS and aldehydes matter in fertilizer plant's sewage effectively, removal effect is better, reaches emission standard.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (6)
1. one kind for repairing the microbial preparation of fertilizer plant's sewage, it is characterized in that, described microbial preparation is prepared as follows and forms: step 1) prepare sorbent material, step 2) prepare microbe carbon source, step 3) prepare microbe carrier, step 4) prepare microbial preparation.
2. microbial preparation according to claim 1, is characterized in that, described microbial preparation is prepared as follows and forms:
Step 1) prepare sorbent material: first zeolite is carried out pulverization process, obtain the zeolite powder of particle diameter between 50-100 order, then mix according to the weight ratio of 1: 2: 2 with silicon-dioxide, humic acids, to obtain final product;
Step 2) prepare microbe carbon source: rice straw is dried, is ground into rice straw powder with pulverizer; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned rice straw powder and peanut hull meal are added in stirred pot, then dregs of beans and water is added, after the speed of 500 revs/min stirs 15 minutes, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30 minutes, last naturally cooling and get final product; The mass ratio of rice straw powder, peanut hull meal, dregs of beans and water is 2: 2: 3: 5;
Step 3) prepare microbe carrier: first control chitosan, trehalose and diatomaceous particle diameter and be 100-200 order, then by chitosan, trehalose, diatomite and glycerine according to 1: 1: 1: 2 mass ratio mix, to obtain final product;
Step 4) prepare microbial preparation: it is 1 × 10 that micrococcus luteus, enterococcus faecalis, Rhodococcus ruber, Acinetobacter bauamnnii, the brilliant Arthrobacter of one-tenth, many glutinous series bacillus and Pseudomonas fluorescens are trained concentration respectively
8the bacterium liquid of individual/ml, according to the volume ratio mixing of 7: 5: 5: 3: 2: 1: 1, then with step 2) microbe carbon source prepared compares mixing and stirring according to the quality of 1: 1, cultivate 12 hours for 30 DEG C, add step 1 subsequently) sorbent material prepared and step 3) microbe carrier prepared, 100 turns/min stirs 3min, finally carry out cryodrying, drying temperature is 20-22 DEG C, and after dry, water content is 10-12%, to obtain final product.
3. microbial preparation according to claim 2, is characterized in that, described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442; Described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) is ATCC 29212; Described Rhodococcus ruber is Rhodococcus ruber (Rhodococcus ruber) ATCC 15906; Described Acinetobacter bauamnnii is Acinetobacter bauamnnii (Acinetobacter baumanii) is ATCC 19606; The brilliant Arthrobacter of described one-tenth is for becoming brilliant Arthrobacter (Arthrobacter crystallopoietes) ATCC 15481; Described many glutinous series bacillus are many glutinous series bacillus (Paenibacillus polymyxa) ATCC 842; Described Pseudomonas fluorescens is Pseudomonas fluorescens (P.Fluorescens) ATCC 49642.
4. the microbial preparation according to Claims 2 or 3, is characterized in that, described step 4) in, the mass ratio of sorbent material, microbe carrier carrier and microbe carbon source is 1: 2: 5.
5. microbial preparation according to claim 4, is characterized in that, the particle diameter of described silicon-dioxide or humic acids is 50-100 order.
6. the microbial preparation let alone described according to claim 1-5 is repairing the application in sewage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510038477.3A CN104528955B (en) | 2015-01-22 | 2015-01-22 | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510038477.3A CN104528955B (en) | 2015-01-22 | 2015-01-22 | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104528955A true CN104528955A (en) | 2015-04-22 |
| CN104528955B CN104528955B (en) | 2016-08-24 |
Family
ID=52844649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510038477.3A Expired - Fee Related CN104528955B (en) | 2015-01-22 | 2015-01-22 | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104528955B (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132345A (en) * | 2015-10-27 | 2015-12-09 | 淮海工学院 | Enterococcus sp. Y12 and application thereof |
| CN106148223A (en) * | 2016-05-06 | 2016-11-23 | 国家海洋局第海洋研究所 | A kind of arctic sanitary sewage efficient cryogenic degradation bacteria and screening technique thereof and application |
| CN106862260A (en) * | 2017-03-10 | 2017-06-20 | 刘晨 | A kind of biochemical process for administering Cadmium Pollution in Soils |
| CN106929453A (en) * | 2017-04-11 | 2017-07-07 | 内蒙古阜丰生物科技有限公司 | Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof |
| CN106964647A (en) * | 2017-06-06 | 2017-07-21 | 临沂大学 | A kind of method of utilization composite bacteria agent remediating lead-contaminated soil |
| CN107446862A (en) * | 2017-09-06 | 2017-12-08 | 北京双龙阿姆斯科技有限公司 | Microbial bacterial agent of degrading pesticide residues and preparation method thereof |
| CN107446861A (en) * | 2017-09-06 | 2017-12-08 | 北京双龙阿姆斯科技有限公司 | Microbial bacterial agent of degraded heavy-metal residual and preparation method thereof |
| CN107557318A (en) * | 2017-09-25 | 2018-01-09 | 黄灵君 | For removing the microorganism formulation of hydrogen sulfide malodorant |
| CN109650642A (en) * | 2017-10-11 | 2019-04-19 | 卢松 | A kind of environment-protective process that xanthan gum industrial wastewater is effectively treated |
| CN110499262A (en) * | 2018-05-17 | 2019-11-26 | 卢松 | Purification absorbs the compound of slurry-spraying pelletizing flue gas |
| CN110902849A (en) * | 2020-02-10 | 2020-03-24 | 广州赛太特生物医学科技有限公司 | Method for treating industrial wastewater by using biological adsorbent |
| CN110921850A (en) * | 2020-02-10 | 2020-03-27 | 广州赛太特生物医学科技有限公司 | Method for treating sewage by using microorganisms |
| CN112607954A (en) * | 2020-11-24 | 2021-04-06 | 安徽广信农化股份有限公司 | Novel process for reducing hexazinone wastewater |
| CN115044580A (en) * | 2022-08-03 | 2022-09-13 | 广州微立旺生物科技有限公司 | Compound microbial agent for removing sulfide and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475161B (en) * | 2017-09-25 | 2021-07-20 | 杭州富阳佳畅机械有限公司 | Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6325934B1 (en) * | 2000-05-09 | 2001-12-04 | James Francis Tobey, Jr. | Enzyme and bacterial combination in a slowly dissolvable matrix for septic tanks, grease traps and waste treatment |
| CN103409404A (en) * | 2013-08-23 | 2013-11-27 | 尹梦珍 | Biological preparation for treating domestic sewage |
| CN103739085A (en) * | 2013-12-20 | 2014-04-23 | 大连赛姆生物工程技术有限公司 | Holothurian culture water body remediation agent prepared by mixed fermentation process and preparation method and application thereof |
| CN104152374A (en) * | 2014-07-10 | 2014-11-19 | 河南恒野农牧股份有限公司 | Microbial preparation for purifying breeding wastewater |
-
2015
- 2015-01-22 CN CN201510038477.3A patent/CN104528955B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6325934B1 (en) * | 2000-05-09 | 2001-12-04 | James Francis Tobey, Jr. | Enzyme and bacterial combination in a slowly dissolvable matrix for septic tanks, grease traps and waste treatment |
| CN103409404A (en) * | 2013-08-23 | 2013-11-27 | 尹梦珍 | Biological preparation for treating domestic sewage |
| CN103739085A (en) * | 2013-12-20 | 2014-04-23 | 大连赛姆生物工程技术有限公司 | Holothurian culture water body remediation agent prepared by mixed fermentation process and preparation method and application thereof |
| CN104152374A (en) * | 2014-07-10 | 2014-11-19 | 河南恒野农牧股份有限公司 | Microbial preparation for purifying breeding wastewater |
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132345A (en) * | 2015-10-27 | 2015-12-09 | 淮海工学院 | Enterococcus sp. Y12 and application thereof |
| CN106148223A (en) * | 2016-05-06 | 2016-11-23 | 国家海洋局第海洋研究所 | A kind of arctic sanitary sewage efficient cryogenic degradation bacteria and screening technique thereof and application |
| CN106148223B (en) * | 2016-05-06 | 2020-04-21 | 国家海洋局第一海洋研究所 | Arctic domestic sewage low-temperature efficient degrading bacterium and screening method and application thereof |
| CN106862260A (en) * | 2017-03-10 | 2017-06-20 | 刘晨 | A kind of biochemical process for administering Cadmium Pollution in Soils |
| CN106929453A (en) * | 2017-04-11 | 2017-07-07 | 内蒙古阜丰生物科技有限公司 | Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof |
| CN106929453B (en) * | 2017-04-11 | 2020-06-26 | 内蒙古阜丰生物科技有限公司 | Biological agent for treating monosodium glutamate fermentation mother liquor and preparation method thereof |
| CN106964647A (en) * | 2017-06-06 | 2017-07-21 | 临沂大学 | A kind of method of utilization composite bacteria agent remediating lead-contaminated soil |
| CN107446861A (en) * | 2017-09-06 | 2017-12-08 | 北京双龙阿姆斯科技有限公司 | Microbial bacterial agent of degraded heavy-metal residual and preparation method thereof |
| CN107446861B (en) * | 2017-09-06 | 2024-02-27 | 北京双龙阿姆斯科技有限公司 | Microbial agent for degrading heavy metal residues and preparation method thereof |
| CN107446862B (en) * | 2017-09-06 | 2023-12-15 | 北京双龙阿姆斯科技有限公司 | Microbial agent for degrading pesticide residues and preparation method thereof |
| CN107446862A (en) * | 2017-09-06 | 2017-12-08 | 北京双龙阿姆斯科技有限公司 | Microbial bacterial agent of degrading pesticide residues and preparation method thereof |
| CN107557318A (en) * | 2017-09-25 | 2018-01-09 | 黄灵君 | For removing the microorganism formulation of hydrogen sulfide malodorant |
| CN107557318B (en) * | 2017-09-25 | 2021-07-20 | 杭州富阳佳畅机械有限公司 | Microbial preparation for removing hydrogen sulfide malodorous substance |
| CN109650642A (en) * | 2017-10-11 | 2019-04-19 | 卢松 | A kind of environment-protective process that xanthan gum industrial wastewater is effectively treated |
| CN110499262A (en) * | 2018-05-17 | 2019-11-26 | 卢松 | Purification absorbs the compound of slurry-spraying pelletizing flue gas |
| CN110902849A (en) * | 2020-02-10 | 2020-03-24 | 广州赛太特生物医学科技有限公司 | Method for treating industrial wastewater by using biological adsorbent |
| CN110921850B (en) * | 2020-02-10 | 2020-06-05 | 广州赛太特生物医学科技有限公司 | Method for treating sewage by using microorganisms |
| CN110921850A (en) * | 2020-02-10 | 2020-03-27 | 广州赛太特生物医学科技有限公司 | Method for treating sewage by using microorganisms |
| CN112607954A (en) * | 2020-11-24 | 2021-04-06 | 安徽广信农化股份有限公司 | Novel process for reducing hexazinone wastewater |
| CN115044580A (en) * | 2022-08-03 | 2022-09-13 | 广州微立旺生物科技有限公司 | Compound microbial agent for removing sulfide and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104528955B (en) | 2016-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104528955B (en) | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage | |
| CN104630101B (en) | It is a kind of to be used to handle biological agent of ammonia-nitrogen sewage and preparation method thereof | |
| CN101899397B (en) | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application | |
| CN106754461B (en) | Biological floc and preparation method and application thereof | |
| CN102616941B (en) | Sea cucumber microecological water quality conditioning agent and method for preparing same | |
| CN104478098B (en) | For the treatment of the microbial preparation of coal chemical industrial waste water | |
| CN103449680B (en) | Method for removing pollutants in sewage | |
| CN102888374B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN102888345B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN103373762B (en) | Biological denitrification method for salt-containing sewage | |
| CN104086001B (en) | Treatment process applicable to industrial wastewater and application of industrial wastewater in preparation of bacterial manure | |
| CN106635861B (en) | A kind of de- COD denitrification microorganism microbial inoculum of salt tolerant and preparation method thereof | |
| CN104152374A (en) | Microbial preparation for purifying breeding wastewater | |
| CN105505841A (en) | Microorganism bacterium agent for treating industrial wastewater pollution | |
| CN104528956B (en) | A kind of composite bacteria agent capable for administering coal chemical industry sewage | |
| CN104628225A (en) | Treatment method of ammonia-nitrogen-containing industrial wastewater | |
| CN105060495A (en) | Biological agent for treatment of high ammonia-nitrogen industrial sewage | |
| CN105039228A (en) | Biological agent for glutamate wastewater treatment | |
| CN104973696A (en) | Preparation technology of biological agent for restoring industrial sewage | |
| CN105000674A (en) | Method used for processing environmental geology industrial wastewater via biotechnology | |
| CN103923867A (en) | Mixed bacterial colony microbial preparation and application thereof in treatment of wastewater containing nitrate nitrogen | |
| CN104891678A (en) | Biological agent used for treating high-concentration waste water produced by amino acid fermentation | |
| CN102465101B (en) | Denitrification bacterium preparation capable of utilizing nitrite to realize denitrification and use thereof | |
| CN103305443B (en) | Preparation and method for restoring ammonia nitrogen-containing industrial sewage | |
| CN107460148A (en) | A kind of preparation method and application of bacillus subtilis bacterium powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Cao Junping Inventor before: Gong Yanhua Inventor before: Chen Jing |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160413 Address after: 276001 room 23, environmental protection bureau, No. 512, Beijing Road, Linyi new North District, Shandong Applicant after: Cao Junping Address before: 276001 room 23, environmental protection bureau, No. 506, Beijing Road, Linyi new North District, Shandong Applicant before: Gong Yanhua |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Wei Inventor after: Zhao Fei Inventor before: Cao Junping |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170927 Address after: 063000 Hebei Tangshan City Road North District Construction North Road Jianshe Road Red Star Building 44 Building 1 gate 203 Co-patentee after: Zhao Fei Patentee after: Liu Wei Address before: 276001 room 23, environmental protection bureau, No. 512, Beijing Road, Linyi new North District, Shandong Patentee before: Cao Junping |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180420 Address after: 310018 No. 4 standard workshop No. 1, No. 1, 8 Avenue, Hangzhou economic and Technological Development Zone, Hangzhou, Zhejiang. Patentee after: Hangzhou lubricant Science Equipment Co., Ltd. Address before: 063000 Hebei Tangshan City Road North District Construction North Road Jianshe Road Red Star Building 44 Building 1 gate 203 Co-patentee before: Zhao Fei Patentee before: Liu Wei |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160824 Termination date: 20190122 |