CN110870484A - Compound pesticide of metarhizium anisopliae and dinotefuran and application thereof - Google Patents
Compound pesticide of metarhizium anisopliae and dinotefuran and application thereof Download PDFInfo
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Abstract
The invention discloses a compound pesticide of Metarhizium anisopliae and dinotefuran and application thereof. The invention firstly provides a compound insecticide which contains metarhizium anisopliae and dinotefuran. The metarhizium anisopliae mutant strain MaUV-1 has the characteristics of ultraviolet resistance and high temperature resistance, and has good compatibility with dinotefuran, the dinotefuran does not influence spore germination and hypha growth of the metarhizium anisopliae mutant strain MaUV-1, and a compound insecticide obtained by compounding the dinotefuran and the metarhizium anisopliae mutant strain MaUV-1 has a synergistic interaction effect, so that the control effect on rice planthoppers and rice leafhoppers can be remarkably improved, the quick-acting property of insecticidal effect is improved, and the using amount of a chemical pesticide dinotefuran is reduced; in addition, the compound pesticide has stable prevention and control effect on rice pests, is not influenced by field environmental factors such as high temperature, strong ultraviolet rays and the like, and has good application prospect in prevention and control of rice planthoppers and rice leafhoppers.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a metarhizium anisopliae (Metarhizium anisopliae) and dinotefuran compound pesticide and application thereof.
Background
Rice planthoppers and rice leafhoppers are important migratory flying pests on rice, fly back and forth between southern China and middle rice planting areas every year, nymphs and adults of the rice plant pests gather at the positions of stems and the like of the rice and stab and absorb rice-eating juice, and simultaneously spread virus diseases such as rice dwarf and the like, and when the diseases are serious, the rice can wither and yellow and fall down, and finally the rice is reduced in yield in a large area, even is harvested absolutely. Dinotefuran is a novel super nicotine pesticide, has the characteristics of contact poisoning, stomach toxicity, quick action and the like, and has long lasting period and wide insecticidal spectrum. Dinotefuran can be quickly transmitted to each part of the plant, has good control effect on sucking mouthparts pests such as aphids, leafhoppers, plant hoppers, thrips, whiteflies and the like, and has low toxicity on mammals. In recent years, the extensive and massive use of pesticides such as dinotefuran on rice results in high-level drug resistance of rice planthopper, rice leafhopper and other rice pests, serious chemical pesticide residue in agricultural products, and simultaneously, the natural enemies of the pests are killed, and the ecological system of the rice field is destroyed.
Metarhizium anisopliae is one of important entomopathogenic fungi, has a completely new action mechanism different from that of the existing chemical insecticide, has the advantages of no pollution to the environment and no residue, and is commonly used for preventing and treating pests such as rice planthopper, rice stem borer, diamondback moth and the like. However, field application practices show that the control effect of live microbial insecticides such as metarhizium anisopliae is unstable and is easily influenced by field environmental conditions such as strong ultraviolet rays and high temperature in southern areas. Therefore, the pesticide resistance problem of rice pests such as rice planthoppers, rice leafhoppers and the like is solved, and the pesticide has important significance for efficiently preventing and controlling the rice pests and improving the yield and the quality of rice.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a compound pesticide of metarhizium anisopliae and dinotefuran and application thereof.
The invention aims to provide a compound insecticide.
The invention also aims to provide application of the compound pesticide in controlling rice planthoppers/rice leafhoppers or preparing a biological agent for controlling the rice planthoppers/the rice leafhoppers.
Still another object of the present invention is to provide a biological agent for controlling rice planthopper/rice leafhopper.
The above purpose of the invention is realized by the following technical scheme:
the invention firstly provides a compound insecticide which contains metarhizium anisopliae and dinotefuran.
Preferably, the mass ratio of the metarhizium anisopliae to the dinotefuran is 100: 6.67-26.67.
More preferably, the mass ratio of the metarhizium anisopliae to the dinotefuran is 100: 12-16.
Still more preferably, the mass ratio of the metarhizium anisopliae to the dinotefuran is 100: 16.
Preferably, the metarhizium anisopliae is metarhizium anisopliae mutant strain MaUV-1 which is preserved in China Center for Type Culture Collection (CCTCC) in 2016, 5, 12 and has a preservation number of CCTCC NO: m2016250, the preservation address is Wuhan, Wuhan university in China.
Preferably, the metarhizium anisopliae mutant strain MaUV-1 is spore powder of the metarhizium anisopliae mutant strain MaUV-1.
The metarhizium anisopliae mutant strain MaUV-1 has better biocompatibility with dinotefuran, and the compound insecticide obtained by compounding the dinotefuran with the metarhizium anisopliae mutant strain MaUV-1 in a sub-dose amount has obvious control effect on rice planthoppers and rice leafhoppers, can improve the quick-acting property of a fungal insecticide, reduce the using amount of chemical pesticides and reduce the chemical pesticide residues in agricultural products; meanwhile, the anti-ultraviolet and high-temperature-resistant metarhizium anisopliae mutant strain MaUV-1 has stable control effect on rice pests such as rice planthoppers, rice leafhoppers and the like, is not influenced by field environmental conditions such as high temperature, strong ultraviolet rays and the like in southern areas, has no pollution to ecological environment, and can continuously control the rice pests; therefore, the following should be within the scope of the present invention:
the compound pesticide is applied to controlling rice planthoppers/rice leafhoppers or preparing a biological preparation for controlling the rice planthoppers/the rice leafhoppers.
In addition, the invention also provides a biological agent for preventing and controlling rice planthoppers/rice leafhoppers, which consists of a main agent and an auxiliary agent; the main agent comprises metarhizium anisopliae mutant strain MaUV-1 and dinotefuran, and the auxiliary agent comprises stabilizer, auxiliary agent and filler.
Preferably, the biological agent comprises, in mass percent: 38 to 64 percent of main agent, 0.3 to 0.8 percent of stabilizing agent, 12 to 16 percent of auxiliary agent and 19.2 to 47.5 percent of filler.
Preferably, the stabilizer is nano aluminum hydroxide or ascorbic acid.
Preferably, the auxiliary agent is any one or more of tween-80, MorwetEF-W, sodium carboxymethylcellulose, a wetting agent LX, a damantofuran DK8, sodium lignosulphonate, span-20 or OP-10.
Preferably, the filler is any one or more of diatomite, attapulgite or paraffin oil.
Preferably, the biological preparation is in the form of wettable powder or oil.
The invention has the following beneficial effects:
the invention provides a compound pesticide of metarhizium anisopliae and dinotefuran and application thereof. The compound pesticide takes metarhizium anisopliae and dinotefuran as effective components, the metarhizium anisopliae mutant strain MaUV-1 has good compatibility with dinotefuran, and the low-concentration dinotefuran and metarhizium anisopliae mutant strain MaUV-1 have synergistic interaction effect after being compounded, so that the pesticidal effect on rice planthopper, rice leafhopper and other rice pests is obviously improved, the using amount of the chemical pesticide dinotefuran is reduced, the drug resistance of the rice pests on the chemical pesticide dinotefuran can be improved, the growth speed of the drug resistance of the rice planthopper, rice leafhopper and other rice pests on the existing chemical pesticide is slowed down, and the residue of the chemical pesticide is reduced; the compound pesticide has stable control effect on rice pests, is not influenced by field environmental factors such as high temperature, strong ultraviolet rays and the like in southern areas, is beneficial to protecting natural enemies of the pests, continuously controls the damage of the rice pests, promotes the improvement of the yield and the quality of the rice, has no pollution to the environment, and has good application prospect.
Drawings
FIG. 1 is a diagram showing symptoms of Bemisia albopictus infected with Metarhizium anisopliae mutant strain MaUV-1.
FIG. 2 is a graph showing the results of the ultraviolet resistance test of metarhizium anisopliae mutant strain MaUV-1.
FIG. 3 is a high temperature resistance test result diagram of metarhizium anisopliae mutant strain MaUV-1.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 acquisition of Metarhizium anisopliae mutant Strain MaUV-1 and infestation on Rice planthopper
The inventor group collects larvae of naturally infected scarab (Phyllopertha horticola L.) in Tibet area, and obtains the wild strain of the metarhizium anisopliae after separation and screening from the larvae. In later-period field application, the strain is found to have unstable control effect on pests and is easily influenced by field environmental conditions such as strong ultraviolet rays, high temperature and the like in southern areas; therefore, the wild strain is irradiated by ultraviolet rays to induce the wild strain to generate variation, and the mutant strain MaUV-1 of the metarhizium anisopliae with ultraviolet resistance and high temperature resistance is finally obtained through screening and purification and is preserved in the China Center for Type Culture Collection (CCTCC) in 2016, 5, 12 and the preservation number is CCTCC NO: m2016250, the preservation address is Wuhan, Wuhan university in China.
The symptom graph of the metarhizium anisopliae after being infected by the metarhizium anisopliae mutant strain MaUV-1 is shown in figure 1, and it can be seen that the metarhizium anisopliae mutant strain MaUV-1 can effectively infect the metarhizium anisopliae and cause a great amount of death of the metarhizium anisopliae (A graph in figure 1), white hyphae can be seen on the polypide of the metarhizium anisopliae on 3-5 days after infection (B graph in figure 1), and dark green spore powder can be seen on the polypide of the metarhizium anisopliae on 8-10 days after infection (C graph in figure 1).
Example 2 compatibility determination of Metarhizium anisopliae mutant Strain MaUV-1 for dinotefuran
1. Experimental methods
Metarhizium anisopliae mutant strain MaUV-1 is inoculated to a Chachi's culture medium (30.0 g of sucrose and sodium nitrate (NaNO)3)3.0g of dipotassium hydrogen phosphate (K)2HPO4)1.0g magnesium sulfate (MgSO)4.7H2O)0.5g, chlorinePotassium chloride (KCl)0.5g, ferrous sulfate (FeSO)4)0.01g and 20g of agar powder, adding water to make the total volume be 1000mL), culturing in a constant temperature box (L: D ═ 14:10) at 26 +/-1 ℃ until sporulation occurs, collecting spores by using 0.1% Tween-80 sterile water, mixing fungal spores and dinotefuran in a Chase culture solution to prepare suspensions containing dinotefuran (0.1x, 0.5x and 1.0x, wherein 1.0x represents 1.0 time of the conventional use concentration) and fungal spores, and performing a slide germination test;
dropping the suspension containing the dinotefuran and the fungal spores with different concentrations on a sterile glass slide, placing the sterile glass slide on a culture dish with filter paper laid at the bottom, adding sterile water for moisturizing (100, RH), culturing for 21h, 24h and 27h, and performing microscopic examination to calculate the spore germination rate (the spore germination rate is germinated spore number/total spore number multiplied by 100%). Each treatment was repeated 3 times. All the test data are processed and completed on a data processing software SAS system.
2. Results of the experiment
The results of the spore germination rates of the metarhizium anisopliae mutant strain MaUV-1 in dinotefuran solutions with different concentrations are shown in Table 1, and it can be seen that compared with a control group, the spore germination rates of the metarhizium anisopliae mutant strain MaUV-1 are remarkably reduced in dinotefuran solutions with 21h, 1.0x and 0.5x times of the conventional concentrations, while the spore germination of the metarhizium anisopliae mutant strain MaUV-1 is not influenced by the dinotefuran with 0.1x times of the conventional concentrations; after 27h, the spore germination rates of the dinotefuran solutions with different concentrations are not significantly different from those of a control group, and the spore germination rates can reach over 86.5%.
The above results illustrate that: within 21h, dinotefuran with the conventional concentration of 1.0x and 0.5x has certain inhibition effect on the spore germination rate of metarhizium anisopliae mutant strain MaUV-1, dinotefuran with the conventional concentration of 0.5x and 0.1x has no inhibition effect on the spore germination rate of mutant strain MaUV-1 after 24h, and dinotefuran has no inhibition effect on the spore germination of fungi after 27h, which shows that dinotefuran only has transient inhibition effect on the spore germination of fungi, and the inhibition effect gradually disappears along with the prolonging of time. The results show that the metarhizium anisopliae mutant strain MaUV-1 has good compatibility with dinotefuran.
TABLE 1 spore germination rate results of metarhizium anisopliae mutant strain MaUV-1 in dinotefuran solutions of different concentrations
| Time of day | Control zone | Dinotefuran (1.0x) | Dinotefuran (0.5x) | Dinotefuran (0.1x) |
| 21h | 58.1±2.9a | 42.9±3.6c | 50.7±2.8b | 57.6±4.1a |
| 24h | 83.4±3.3a | 61.3±4.1b | 80.5±3.7a | 82.4±3.5a |
| 27h | 90.6±4.6a | 86.5±4.6a | 87.9±5.3a | 89.6±5.2a |
Note: the same letters after the same number in the same column in the table indicate that the difference is not significant (DMRT method); dinotefuran (1x), dinotefuran (0.5x) and dinotefuran (0.1x) respectively represent the use dose of dinotefuran as the field conventional use dose and the field conventional use dose of 0.5 and 0.1 times.
Example 3 high temperature and ultraviolet resistance test of Metarhizium anisopliae mutant Strain MaUV-1
1. Experimental methods
Inoculating conidia of wild metarhizium anisopliae (WT) and mutant (MaUV-1) to PDA plate, culturing, collecting spores of all strains with 0.1% Tween-80 sterile water, and making into 1.0 × 106spore/mL spore suspension is placed under ultraviolet irradiation for 0 and 40min, then 5mL spore suspension is added into a triangular flask filled with 200mL Chachi culture solution, the culture is carried out at 26 +/-1 ℃ and 200rpm, spore germination is observed and recorded at 22h, 25h and 28h, and the germination standard is that the length of a bud tube exceeds half of the diameter of the spore. All treatments were repeated 3 times. The ultraviolet resistance of the metarhizium anisopliae mutant strain MaUV-1 is determined by comparing the difference of the strains at 22h, 25h and 28h in spore germination rate.
In addition, spores of all strains were collected with 0.1% Tween-80 sterile water and formulated into 1.0X 106spore/mL, then 5mL of spore suspension is added into a triangular flask containing 200mL of Chashi culture solution, the mixture is cultured under the conditions of 28 +/-1 ℃, 34 +/-1 ℃, 35 +/-1 ℃, 37 +/-1 ℃, 38 +/-1 ℃ and 200rpm, the germination condition of the spores is observed and recorded at 25h and 28h, and the germination standard is that the length of a bud tube exceeds half of the diameter of the spores. All treatments were repeated 3 times. The high temperature resistance of the metarhizium anisopliae mutant strain MaUV-1 is determined by comparing the difference of spore germination rates of the strains at different temperatures in the 25 th and 28 th hours.
2. Results of the experiment
The ultraviolet resistance test result of the metarhizium anisopliae mutant strain MaUV-1 is shown in figure 2, and it can be seen from the figure that after the metarhizium anisopliae mutant strain MaUV-1 is irradiated by ultraviolet rays for 40min, the difference between the spore germination rates of 22h, 25h and 28h and a control group (irradiated by ultraviolet rays for 0min) is not significant, while after the wild strain is irradiated by the ultraviolet rays for 40min, the difference between the spore germination rate of the wild strain and the control group is significant, and the spore germination rate of the wild strain after the ultraviolet rays are treated is significantly reduced.
The result of the high temperature resistance test of the metarhizium anisopliae mutant strain MaUV-1 is shown in figure 3, and it can be seen that the spore germination rate of the metarhizium anisopliae mutant strain MaUV-1 at the high temperature of 37 ℃ is more than 30%, while the spores of the wild strain do not germinate at the temperature of 35 ℃.
The above results illustrate that: the metarhizium anisopliae mutant strain MaUV-1 has good ultraviolet resistance, spores can germinate at a high temperature of 37 ℃, and the metarhizium anisopliae mutant strain has good high-temperature resistance.
Example 450% wettable powder of Metarhizium anisopliae mutant strain MaUV-1 and dinotefuran
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran comprises the following specific formula components: 50% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 8% dinotefuran (a commercial raw drug), 0.8% nano-aluminum hydroxide, 5% tween-80, 4% Morwet EF-W, 5% sodium carboxymethylcellulose and diatomite which are supplemented to 100% to prepare 50% metarhizium anisopliae mutant strain MaUV-1 and dinotefuran wettable powder; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to dinotefuran is 100: 8.
Example 530% wettable powder of Metarhizium anisopliae mutant strain MaUV-1 and dinotefuran
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran comprises the following specific formula components: 30% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 8% dinotefuran (a commercial raw drug), 0.5% nano-aluminum hydroxide, 3% tween-80, 5% Morwet EF-W, 6% sodium carboxymethylcellulose and diatomite which are supplemented to 100% to prepare 30% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to dinotefuran is 100: 26.67.
Example 660% wettable powder of Metarhizium anisopliae mutant strain MaUV-1 and dinotefuran
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran comprises the following specific formula components: 60% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 4% dinotefuran (a commercial raw drug), 0.8% nano-aluminum hydroxide, 6% tween-80, 5% Morwet EF-W, 5% sodium carboxymethylcellulose and diatomite which are supplemented to 100% to prepare 60% metarhizium anisopliae mutant strain MaUV-1 and dinotefuran wettable powder; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to dinotefuran is 100: 6.67.
Example 750% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran comprises the following specific formula components: 50% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 6% dinotefuran (a commercial raw drug), 0.3% ascorbic acid, 3% wetting agent LX, 6% suspending agent DK8, 5% sodium lignosulfonate, and attapulgite to 100% to prepare 50% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to dinotefuran is 100: 12.
Example oil solution of 850% Metarhizium anisopliae mutant strain MaUV-1 and dinotefuran
An oil solution of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran comprises the following specific formula components: 50% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 6% dinotefuran (a commercial raw drug), 0.8% nano-aluminum hydroxide, 4% span-20, 3% tween-80, 5% OP-10, and paraffin oil to make up to 100%, to prepare 50% oil solution of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to dinotefuran is 100: 12.
Comparative example 1 Metarhizium anisopliae mutant strains MaUV-1 and dinotefuran in the compounded agent
A wettable powder, except there is no metarhizium anisopliae mutant strain MaUV-1 and dinotefuran, the other formulation ingredient content is basically the same as example 4; 0.8% of nano aluminum hydroxide, 5% of tween-80, 4% of Morwet EF-W, 5% of sodium carboxymethylcellulose and the diatomite which are supplemented to 100% to prepare wettable powder (the using concentration of the metarhizium anisopliae mutant strain MaUV-1 and the dinotefuran is 0).
Comparative example 2 absence of dinotefuran in the combination
A wettable powder, except that no dinotefuran is added, the contents of the other formulation components are basically the same as that in example 4; the specific formula comprises the following components in percentage by weight: 60% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 0.8% of nano aluminum hydroxide, 5% of tween-80, 4% of Morwet EF-W, 5% of sodium carboxymethylcellulose and diatomite which are supplemented to 100% to prepare wettable powder (the using concentration of metarhizium anisopliae mutant strain MaUV-1 is 3x 10)7spores/mL).
Comparative example 3 lack of Metarhizium anisopliae mutant strain MaUV-1 in compounded agent
A wettable powder, except there is no Metarhizium anisopliae mutant strain MaUV-1, the other formulation component content is basically the same as example 4; the specific formula comprises the following components in percentage by weight: 8% dinotefuran (commercial raw pesticide), 0.8% nano-aluminum hydroxide, 5% tween-80, 4% Morwet EF-W, 5% sodium carboxymethylcellulose and diatomite which are supplemented to 100% to prepare wettable powder (the actual use concentration of the dinotefuran is 40 mug/mL).
Comparative example 4
20% dinotefuran, the conventional dosage in the field (3.0 g/mu/45 kg water, the actual use concentration of dinotefuran is 133.3 mug/mL).
Application example 1 toxicity determination of compound insecticide on sogatella furcifera
Carrying out toxicity determination experiments on the compound pesticide of metarhizium anisopliae mutant strains MaUV-1 and dinotefuran prepared in the embodiments 4-8 of the invention and the wettable powder prepared in the comparative examples 1-4 to determine the toxicity of the compound pesticide on Sogatella furcifera; the specific experimental methods and experimental results are as follows:
1. experimental methods
The test plant is rice (Oryza sativa L.), potted seedlings (the diameter of a pot is 30cm, the height of the seedlings is 40cm, 3 clusters/pot and 6-13 plants/cluster) are placed in an insect rearing cage, adult sogatella furcifera is inoculated, all the adult sogatella furcifera is driven out after spawning, the sogatella furcifera grows to 4-year-old nymphs for later use, and all the tests are carried out in a net room (32-37 ℃);
diluting the compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran prepared in the embodiments 4-8 and the wettable powder prepared in the comparative examples 1-3 by 2000 times to respectively obtain mixed solutions with different concentrations; the dinotefuran in comparative example 4 is prepared into liquid medicine with the concentration (133.3 mug/mL) which is conventionally used in the field, then the liquid medicine is respectively sprayed on the leaves and stems of the rice of different treatment groups by adopting a spraying method, each 6-pot seedling is treated when the rice is fully stained with the liquid medicine mist drops but not dropped, the effect of 5 and 10 days after the pesticide is investigated, the corrected death rate of the sogatella furcifera is calculated, and the whole experiment is repeated for 4 times. The corrected mortality rate was calculated according to the following formula:
corrected mortality (%) — treatment mortality-control mortality)/(1-control mortality) × 100.
2. Results of the experiment
The toxicity test results of the compound pesticide prepared by the invention to sogatella furcifera are shown in table 2, and it can be seen from the table that when the metarhizium anisopliae mutant strain MaUV-1 and dinotefuran are not contained in the wettable powder (comparative example 1), the control effect of the sogatella furcifera is only 9.4% (5 days) and 11.7% (10 days); when the wettable powder only contains dinotefuran, the control effect of the Sogatella furcifera is 42.6 percent (comparative example 3, the treated area of 40PPM of dinotefuran is 10 days) and 78.6 percent (comparative example 4, the treated area of 133.3PPM of dinotefuran is 10 days) respectively; when the wettable powder only contains metarhizium anisopliae mutant strain MaUV-1 (comparative example 2), the control effect of the sogatella furcifera is 35.1 percent (5 days) and 80.9 percent (10 days); the compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and dinotefuran (example 4-8) has the control effect on sogatella furcifera of 81.6-88.6% (5 days) and 91.0-97.0% (10 days); the control effect of the compound agent on sogatella furcifera is obviously higher than that of the wettable powder prepared in comparative examples 1-3 and obviously higher than that of the conventional field recommended use concentration of dinotefuran in comparative example 4.
In addition, the compound pesticide can prevent sogatella furciferaThe control effect is obviously higher than that of a single agent, the control effect is improved and the insecticidal time is shortened compared with that of a fungal insecticide, and the control effect is improved and the control duration is prolonged compared with that of a chemical pesticide. Wherein the single preparation is mixed with Metarhizium anisopliae (Metarhizium anisopliae MaUV-1) at a concentration of 3x107spore/mL), the effect of the compound pesticide on dinotefuran is improved by 132.5-152.4% (day 5) and 12.5-19.9% (day 10), which shows that the quick-acting property of the pesticide is improved after the dinotefuran is added into the Isaria javanicus pesticide, and the control effect on day 5 is obviously improved by more than 1.3 times. Compared with a chemical pesticide dinotefuran single agent (the using concentration of dinotefuran is 40 mug/mL), the control effect of the compound pesticide on sogatella furcifera is improved by 100-117.2% (day 5) and 113.6-127.7% (day 10), which shows that the control effect is improved by more than 1 time after the chemical pesticide is added with the fungal pesticide, and the effective control time of the pesticide is prolonged. Compared with the chemical pesticide with the conventional dosage, the compound pesticide improves the control effect and reduces the dosage of the chemical pesticide by 233.3 percent.
The results show that the compound pesticide of the metarhizium anisopliae mutant strain MaUV-1 and the dinotefuran prepared by the invention has an obvious control effect on the bemisia alba, the compound of the metarhizium anisopliae mutant strain MaUV-1 and the dinotefuran can play a role in synergy, the control effect of the compound pesticide on the bemisia alba is obviously higher than that of a single agent, the control effect is improved, the pesticidal time is shortened, the control duration time is prolonged, and the using amount of chemical pesticides is reduced.
TABLE 2 toxicity test results of the formulated insecticide against Sogatella furcifera (2000 times dilution of the compounded agent)
Note: the same letters after the same numbers in the same column indicate no significant difference (DMRT method).
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The compound insecticide is characterized by containing metarhizium anisopliae and dinotefuran.
2. The compound pesticide according to claim 1, wherein the mass ratio of the metarhizium anisopliae to the dinotefuran is 100: 6.67-26.67.
3. The compound pesticide according to claim 2, wherein the mass ratio of the metarhizium anisopliae to the dinotefuran is 100: 12-16.
4. The compound pesticide according to claim 3, wherein the mass ratio of the metarhizium anisopliae to the dinotefuran is 100: 16.
5. The compound pesticide according to any one of claims 1 to 4, wherein the metarhizium anisopliae is metarhizium anisopliae mutant strain MaUV-1, which is preserved in the China Center for Type Culture Collection (CCTCC) in 2016, 5, 12 and has a preservation number of CCTCC NO: m2016250.
6. The compound pesticide according to claim 5, wherein the metarhizium anisopliae mutant strain MaUV-1 is spore powder of the metarhizium anisopliae mutant strain MaUV-1.
7. The application of the compound pesticide as claimed in any one of claims 1 to 6 in controlling rice planthoppers/rice leafhoppers or preparing biological agents for controlling the rice planthoppers/the rice leafhoppers.
8. The biological preparation for preventing and controlling rice planthoppers/rice leafhoppers is characterized by consisting of a main agent and an auxiliary agent; the main agent comprises metarhizium anisopliae mutant strain MaUV-1 and dinotefuran, and the auxiliary agent comprises stabilizer, auxiliary agent and filler.
9. The biological agent according to claim 8, characterized in that it comprises, in mass percent: 38 to 64 percent of main agent, 0.3 to 0.8 percent of stabilizing agent, 12 to 16 percent of auxiliary agent and 19.2 to 47.5 percent of filler.
10. The biological preparation according to claim 8 or 9, wherein the biological preparation is in the form of wettable powder or oil.
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