CN109085348B - A kind of method for analyzing body fluid - Google Patents
A kind of method for analyzing body fluid Download PDFInfo
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- CN109085348B CN109085348B CN201710718118.1A CN201710718118A CN109085348B CN 109085348 B CN109085348 B CN 109085348B CN 201710718118 A CN201710718118 A CN 201710718118A CN 109085348 B CN109085348 B CN 109085348B
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- test paper
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
Abstract
The invention discloses a kind of method for analyzing body fluid, to save additional power device, block applied to detection, the test paper that detection card is carried including substrate, the substrate, and encapsulated layer, substrate loading area are directly or indirectly connected to the sample introduction side in test paper area, outlet area is directly or indirectly connected to the sample side that goes out in test paper area, this method comprises: processing encapsulated layer, forms the injection port being connected with atmosphere and gas outlet；Injection port is located at the top in loading area, and gas outlet is located at the top in substrate outlet area；Liquid to be detected is injected to the loading area of substrate by injection port；Liquid to be detected is guided into the test paper into substrate test paper area；After waiting the default reaction time, the test paper in Test paper area.The internal balance with ambient atmosphere of detection card may be implemented in injection port above loading area and the gas outlet above outlet area, avoids the blocking that gas moves liquid flow to be detected, to realize that test paper is loaded.
Description
Technical field
The present invention relates to medical detection field more particularly to a kind of method for analyzing body fluid.
Background technique
Body fluid analysis, such as urinalysis, blood analysis are the important measuring means in clinical examination, especially urine
Analysis, is commonly used for hospital and each medical institutions.The basic mode of urinalysis according to test paper type difference be broadly divided into
Lower two kinds: the first, changed colour according to ingredient in indicator paper block and urine there is a phenomenon where chemical reaction, immersion is urinated using light
The indicator paper block of liquid is irradiated, and detects the color and strength information of its reflected light, to calculate the chemical component in urine
Concentration；Second, antigen in solution is creeped the conversion zone to test paper with solution along test paper, includes in conversion zone
Can with the antibody of antigen binding, due to the combination of antigen and antibody, to form one or more of lines, root in conversion zone
The concentration of each chemical component in urine can be judged according to the color of line.
Urinalysis is carried out using urine analysis system, needs to use urinalysis test paper.Common urinalysis test paper
There is the indicator paper block of multiple rectangles, the corresponding detection project of each indicator paper block.Indicator paper block is to be easy the material of water suction (as filtered
Paper) based on, particular agent is added and is made.Indicator paper block is easy water suction, after test paper immerses urine, indicator paper block sucking urine
Liquid, the chemical component in urine can be chemically reacted with the reagent being previously added in indicator paper block, so that indicator paper block be made to become
Color.
However, due to the corresponding detection project of each indicator paper block, and the detection project of a urinalysis is often more than
One, need repeatedly sample-adding when a kind of existing technical solution is to urinalysis, analytical equipment there need to be a displacement platform, when test
The loading that urine is constantly moved by displacement platform to realize multiple indicator paper blocks, thus under normal conditions the volume of analytical equipment compared with
Greatly, higher cost.To solve this problem, occurred quickly analyzing using micro-fluidic chip in existing another technical solution
During which the analysis means of urine only need to once be loaded, and not need displacement platform.But existing micro-fluidic chip is only used
In carrying indicator paper block, when not having dynamical system, therefore carrying out urinalysis using micro-fluidic chip, it is also necessary to additional dynamic
Power device flows urine in micro-fluidic chip, to increase the volume and cost of analytical equipment.Applicant is same
It describes a kind of detection in the other applications " a kind of substrate and detection card " submitted for one day to block, which may be implemented without additional
Test paper sample-adding in the case of power device.
Summary of the invention
The present invention provides a kind of method for analyzing body fluid, to provide a kind of application method of detection card.
The embodiment of the present invention provides a kind of method for analyzing body fluid, is applied to detection card, the detection card includes substrate, the base
The test paper that plate is carried, and for encapsulating the substrate and the test paper with by the encapsulated layer of the test paper and isolated from atmosphere,
The substrate includes loading area, test paper area and outlet area for carrying test paper, the sample introduction in the loading area and the test paper area
Side is directly or indirectly connected to, and the sample side that goes out in the outlet area and the test paper area is directly or indirectly connected to, the loading area, described
Test paper area and the outlet area are the groove structure being independently arranged on the substrate, this method comprises:
The encapsulated layer is handled, the injection port being connected with atmosphere and gas outlet are formed；The injection port is located at described
The top in loading area, the gas outlet are located at the top in substrate outlet area；
Liquid to be detected is injected to the loading area of the substrate by the injection port；
The liquid to be detected is guided into the test paper into substrate test paper area；
After waiting the default reaction time, the test paper in the test paper area is detected.
Optionally, it is injected before liquid to be detected by the injection port to the loading area of the substrate, further includes:
It is horizontally arranged the detection card.
Optionally, the encapsulated layer includes interior encapsulated layer and outer package layer；The interior encapsulated layer is the covering substrate
Transparent film layer, the region that the interior encapsulated layer corresponds to the outlet area are provided with gas outlet, and the interior encapsulated layer corresponds on described
The region in sample area is provided with injection port；The outer package layer is the packaging bag of the detection card；
The encapsulated layer is handled, injection port, gas outlet is made to be connected with atmosphere, comprising:
The outer package layer is removed, the injection port on the interior encapsulated layer and the gas outlet are exposed.
Optionally, the encapsulated layer is the transparent film layer for covering the substrate, and the encapsulated layer constitutes close with the substrate
Close space；
The encapsulated layer is handled, injection port, gas outlet is made to be connected with atmosphere, comprising:
Position corresponding with the loading area of the substrate is selected on the encapsulated layer and opening is set, and obtains the sample introduction
Mouthful, and, position corresponding with the outlet area of the substrate is selected on the encapsulated layer and opening is set, and obtains the outlet
Mouthful, the injection port, gas outlet are connected with atmosphere.
Optionally, auxiliary projection is provided in the loading area；The auxiliary projection is for propping up the encapsulated layer；
Position corresponding with the loading area of the substrate is selected on the encapsulated layer and opening is set, and obtains the sample introduction
Mouthful, comprising:
The region close to the auxiliary projection is selected on the encapsulated layer and opening is set, and obtains the injection port.
Optionally, gas outlet mark and injection port mark are preset on the encapsulated layer, the gas outlet mark is located at institute
It states above outlet area, the injection port mark is located above the loading area；
Position opening corresponding with the loading area of the substrate, obtains the injection port on the encapsulated layer, and,
Position opening corresponding with the outlet area of the substrate, obtains the gas outlet on the encapsulated layer, comprising:
Region openings on the encapsulated layer where the injection port mark are as the injection port；
Region openings on the encapsulated layer where the gas outlet mark are as the gas outlet.
Optionally, the quantity of the gas outlet mark is corresponding with the quantity in test paper area that the outlet area is connected to；
The position of the gas outlet mark is corresponding with the position in test paper area that the outlet area is connected to.
Optionally, the substrate is with hydrophilic substrate；
The liquid to be detected is guided into the test paper into substrate test paper area, comprising:
The liquid to be detected is guided to the test paper into substrate test paper area by substrate.
Optionally, the detection card further includes with loading area linking area positioned at the test paper area with water suction
The guiding thing of property, one end of the guiding thing are located at the loading area, in the other end of the guiding thing and the test paper area
Test paper be in contact；
The liquid to be detected is guided into the test paper into substrate test paper area, comprising:
The liquid to be detected in the loading area is guided by the guiding thing to the test paper.
Optionally, the substrate further includes the first fluid channel for being connected to the test paper area and the outlet area, and described
The width of one fluid channel is less than the width of the test paper；
After the liquid to be detected to guide to the test paper into substrate test paper area, further includes:
The liquid to be detected amount that the test paper area is accommodated is controlled by the first fluid channel and the test paper area.
Optionally, the substrate further includes the second fluid channel being connected to the loading area and is connected to the test paper area
Third fluid channel；The third fluid channel is the branch in the second fluid channel；
The liquid to be detected is guided to the test paper into substrate test paper area by substrate, comprising:
By the second fluid channel, the liquid to be detected in the loading area is guided to the third fluid and is led to
Road；
By the third fluid channel, the liquid to be detected is guided into the test paper into the test paper area.
Optionally, the third fluid channel is multiple；The test paper area is multiple；Any one third fluid channel with extremely
The sample introduction side in a few test paper area is connected；
By the third fluid channel, the liquid to be detected is guided into the test paper into the test paper area, comprising:
By the multiple third fluid channel, the liquid to be detected is separately directed in the multiple test paper area
Test paper.
In conclusion the embodiment of the present invention provides a kind of method for analyzing body fluid, it is applied to detection card, detection card includes base
The test paper that plate, the substrate are carried, and for package substrate and test paper with by the encapsulated layer of test paper and isolated from atmosphere, substrate packet
Loading area, test paper area and outlet area for carrying test paper are included, loading area is directly or indirectly connected to the sample introduction side in test paper area, out
Gas area is directly or indirectly connected to the sample side that goes out in test paper area, and loading area, test paper area and outlet area are recessed to be independently arranged on substrate
Slot structure, this method comprises: processing encapsulated layer, forms the injection port being connected with atmosphere and gas outlet；Injection port is located at upper
The top in sample area, gas outlet are located at the top in substrate outlet area；Liquid to be detected is injected to the loading area of substrate by injection port；
Liquid to be detected is guided into the test paper into substrate test paper area；After waiting the default reaction time, the test paper in Test paper area.
Due to encapsulating layer package substrate, encapsulated layer need to be handled first to form the injection port that is connected with atmosphere and gas outlet, by
It is provided with the loading area, test paper area and outlet area being directly or indirectly connected to from each other in substrate, therefore is located above loading area
Injection port and the gas outlet above outlet area the internal balance with ambient atmosphere of detection card may be implemented, so as to avoid
The liquid to be detected blocking that gas moves liquid flow to be detected during detecting card internal flow, the in addition water suction of test paper itself
Property, allow liquid to be detected to be impregnated with the test paper in test paper area, to realize that test paper is loaded.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly introduced, it should be apparent that, drawings in the following description are only some embodiments of the invention, for this
For the those of ordinary skill in field, without any creative labor, it can also be obtained according to these attached drawings
His attached drawing.
Fig. 1 is a kind of detection card side view provided in an embodiment of the present invention；
Fig. 2 is a kind of method for analyzing body fluid flow diagram provided in an embodiment of the present invention；
Fig. 3 is a kind of board structure top view provided in an embodiment of the present invention；
Fig. 4 is a kind of detection card structure side view provided in an embodiment of the present invention；
Fig. 5 is a kind of detection card structure top view provided in an embodiment of the present invention；
Fig. 6 is a kind of detection card structure top view provided in an embodiment of the present invention；
Fig. 7 is a kind of detection card structure top view provided in an embodiment of the present invention；
Fig. 8 is a kind of detection card structure top view provided with multiple gas outlets provided by the embodiment of the present invention；
Fig. 9 is a kind of detection card structure top view provided in an embodiment of the present invention；
Figure 10 is a kind of detection card structure top view provided in an embodiment of the present invention；
It is a kind of board structure top view provided in an embodiment of the present invention shown in Figure 11.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with attached drawing to the present invention make into
It is described in detail to one step, it is clear that the described embodiments are only some of the embodiments of the present invention, rather than whole implementation
Example.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
All other embodiment, shall fall within the protection scope of the present invention.
Fig. 1 is a kind of detection card side view provided in an embodiment of the present invention, as shown in Figure 1, detection card includes substrate 1 and envelope
Layer 2 is filled, in addition, further including the test paper that substrate 1 is carried, wherein encapsulated layer 2 is used for the examination that package substrate 1 and substrate 1 are carried
Paper is with by test paper and isolated from atmosphere.Substrate 1 includes loading area, several test paper areas and outlet area for carrying test paper, loading
Area is directly or indirectly connected to the sample introduction side in test paper area, and outlet area is directly or indirectly connected to the sample side that goes out in test paper area, loading area,
Test paper area and outlet area are the groove structure being independently arranged on substrate.
In the detection card provided by the embodiment of the present invention, any test paper area of substrate all has sample introduction side and out sample side,
So-called sample introduction side and the out division of sample side are by liquid when flowing on substrate, and the direction that liquid flows in and out test paper area determines
, specifically, the side that liquid flows into test paper area is sample introduction side, and the side that liquid flows out test paper area is sample side.On substrate
Each area between mode of communicating there is directly connection and two kinds of indirect communication, for example, if loading area and test paper area
Sample introduction side is directly connected to, then the liquid in loading area directly can enter test paper from the loading side in test paper area without other regions
Area, if the sample introduction side indirect communication in loading area and test paper area, the liquid in loading area also need to flow through other regions or structure it
Test paper area could be entered from the sample introduction side in test paper area afterwards.
In the detection card provided by the embodiment of the present invention, the loading area of substrate, several test paper areas and outlet area are only
The groove structure set is erected, the so-called groove structure being independently arranged refers to that loading area, several test paper areas and outlet area are all
Has effigurate specific groove structure, although it is gone back from each other due to loading area, several test paper areas and outlet area
It need to directly or indirectly be connected to, groove structure may not be completely enclosed, but the region shape that groove structure is constituted should be
Explicitly, there is clear boundaries in the division of region shape for loading area, several test paper areas and outlet area.Generally, recessed
The bottom portion of groove shape that the region shape that slot structure is constituted directly reacts for groove structure, optionally, loading area, several examinations
The groove structure bottom in paper area and outlet area is substantially rectangular, due to each groove structure in substrate and other groove knots
Structure is connected, and mode of communicating is varied, therefore the bottom of groove structure may not be one completely regular and closed
Rectangle, but its most of feature that should meet rectangle.
Optionally, a generally rectangular shaped region is collectively formed in loading area, several test paper areas and outlet area, due to common
Detection card be mostly rectangle, a generally rectangular shaped region is collectively formed in loading area, several test paper areas and outlet area can be with
The rectangular shape for adapting to detection card can also be improved the surface utilisation of the substrate of detection card.
Optionally, in the detection card provided by the embodiment of the present invention, the loading area of substrate, several test paper areas and outlet
The groove structure bottom in area is in same level.Using this structure, when can be to avoid being loaded to test paper, groove structure bottom
It rises and falls and bring resistance is flowed to liquid, allow the liquid to more swimmingly flow.
Optionally, in the detection card provided by the embodiment of the present invention, substrate is with hydrophilic substrate.Substrate can be with
Hydrophilic material production is directlyed adopt, hydrophilic treated can also be carried out to the substrate made by non-hydrophilic material, such as impregnated hydrophilic
Solution makes substrate surface covering hydrophilic film to obtain hydrophily, and hydrophilic treated result is not more than 20 with contact angle。It is advisable.Tool
There is hydrophilic substrate surface lower, when so that being loaded to test paper, the liquid of substrate surface produces under the action of surface tension
It has given birth to a kind of the phenomenon that being similar to capillarity, driving liquid flows on the first substrate, to make the stream of liquid in a substrate
It is dynamic more smooth.
Detection card provided in based on the above embodiment, the embodiment of the present invention provides a kind of method for analyzing body fluid, with reality
Now it is loaded without the test paper in the case of additionaling power device.Fig. 2 is a kind of method for analyzing body fluid process provided in an embodiment of the present invention
Schematic diagram, as shown in Figure 2, comprising the following steps:
S201: processing encapsulated layer forms the injection port being connected with atmosphere and gas outlet.
Wherein, injection port is located at the top in the loading area, and gas outlet is located at the top in substrate outlet area.
S202: liquid to be detected is injected to the loading area of substrate by injection port.
S203: liquid to be detected is guided into the test paper into substrate test paper area.
S204: after waiting the default reaction time, the test paper in Test paper area.
Due to encapsulating layer package substrate, encapsulated layer need to be handled first to form the injection port being connected with atmosphere and go out
Port is located at loading since substrate is provided with the loading area being directly or indirectly connected to from each other, test paper area and outlet area
The internal balance with ambient atmosphere of detection card may be implemented in injection port above area and the gas outlet above outlet area, thus
The liquid to be detected blocking that gas moves liquid flow to be detected during detecting card internal flow is avoided, in addition test paper itself
Water imbibition, allow liquid to be detected to be impregnated with the test paper in test paper area, thus realize test paper be loaded.
For example, Fig. 3 is a kind of board structure top view provided in an embodiment of the present invention, as shown in Fig. 2, substrate 1 wraps
Loading area 11, test paper area 12 and outlet area 13 for carrying test paper are included, loading area 11 and the sample introduction side in test paper area 12 directly connect
Logical, outlet area 13 is directly connected to the sample side that goes out in test paper area 12, and loading area 12, test paper area and outlet area are to be independently arranged on substrate
Groove structure.As shown in figure 3, the groove structure bottom substantially square in loading area 11, several test paper areas 12 and outlet area 13
A generally rectangular shaped region is collectively formed in shape, loading area 11, several test paper areas 12 and outlet area 13.
It is different according to the difference of the specific packing forms of detection card to the processing mode of encapsulated layer 2 in S201.Fig. 4
For a kind of detection card structure side view provided in an embodiment of the present invention, in conjunction with Fig. 1 as it can be seen that encapsulated layer 2 includes interior 23 He of encapsulated layer
Outer package layer 24；Interior encapsulated layer 23 is the transparent film layer for covering substrate 1, and outer package layer 24 is the packaging bag of detection card.With Fig. 4 institute
The side view shown is corresponding, and Fig. 5 is a kind of detection card structure top view provided in an embodiment of the present invention, wherein inspection described in Fig. 5
Card is surveyed comprising substrate as shown in Figure 3, the test paper 121 of 1 test paper area 12 of substrate carrying and by interior encapsulated layer 23 and outer package layer
24 encapsulated layers 2 constituted.In Fig. 5, the region in interior 23 counterpart substrate outlet area of encapsulated layer is provided with gas outlet 232, interior encapsulated layer
The region in counterpart substrate loading area is provided with injection port 231.When to the processing of encapsulated layer 2, outer package layer 24 is directly removed, thus cruelly
Injection port 231 and gas outlet 232 in exposing on encapsulated layer 23, so that injection port 231 and gas outlet 232 are connected with atmosphere
It is logical.Wherein, interior encapsulated layer 23 is transparent material, can directly detect in this way to test paper in S204, otherwise, also need elder generation
Encapsulated layer 23 can just detect test paper in removing, and operate relatively complicated.
The embodiment of the present invention also provides another packing forms, and encapsulated layer 2 is the transparent film layer for covering substrate 1, encapsulated layer 2
Confined space is constituted with substrate 1.Fig. 6 is a kind of detection card structure top view provided in an embodiment of the present invention, for using such
The detection card of packing forms, when handling encapsulated layer 2, the 2 selections position corresponding with the loading area 11 of substrate 1 on encapsulated layer
Opening is set and be arranged, injection port is obtained, and, position corresponding with the outlet area 13 of substrate 1 is selected on encapsulated layer 2 and is arranged
Opening obtains gas outlet, and injection port, gas outlet are connected with atmosphere.In the specific implementation process, due to 11 conduct of loading area
The region of sample is injected in substrate 1, area is larger, and the encapsulated layer 2 of film layer material usually has certain elasticity in addition, will
Increase the difficulty that opening is set on encapsulated layer 2, when being such as open in such a way that needle is pricked, encapsulated layer 2 is squeezed by needle point to contact substrate 1
Encapsulated layer 2 may not all be pricked when 11 bottom of loading area.In order to cope with the above problem, optionally, as shown in fig. 7, for the present invention
A kind of detection card structure top view that embodiment provides, detection shown in Fig. 7 are provided with auxiliary in blocking in the loading area 11 of substrate 1
Protrusion 18 is helped, auxiliary projection 18 selects position corresponding with the loading area 11 of substrate 1 for propping up encapsulated layer 2 on encapsulated layer 2
And opening is set, obtain injection port when, on encapsulated layer 2 select close to auxiliary projection 18 region and opening is set, obtain into
Sample mouth, by auxiliary projection to the supporting role of the first encapsulated layer 2 to increase the elastic deformation of encapsulated layer 2, to reduce encapsulation
Influence of the elasticity of layer 2 to opening process.
Further, as shown in Figure 6 and Figure 7, gas outlet mark 22 and injection port mark 21 are preset on encapsulated layer 2, out
Port mark 22 is located at 13 top of outlet area, and injection port mark 21 is located at 11 top of loading area.Opening is set on encapsulated layer 2
When, the region openings on encapsulated layer 2 where injection port mark 21 are as injection port；Gas outlet identifies 22 institutes on encapsulated layer 2
Region openings as gas outlet, pass through injection port mark 21 and gas outlet mark 22 directly calibration injection port and gas outlet
Position simplifies opening manipulation.On this basis, in order to enhance the internal counterbalance effect with atmosphere of detection card, gas outlet identifies 22
Quantity it is corresponding with the quantity in test paper area 12 that outlet area 13 is connected to, gas outlet mark 22 position and outlet area 13 connect
The position in logical test paper area 12 is corresponding, that is to say, that each test paper area being connected with outlet area have one it is right therewith
The gas outlet answered, specifically the structure after opening is as shown in figure 8, Fig. 8 is one kind provided by the embodiment of the present invention provided with multiple
The detection card structure top view of gas outlet, wherein default gas outlet mark 22 is equivalent to gas outlet, and injection port mark 21 is equivalent to
Injection port, as shown in figure 8, removing outside gas outlet 22, there are also gas outlet 22a, gas outlet 22b, gas outlet 22c, gas outlet 22 goes out
The quantity in multiple test paper areas 12 that port 22a, gas outlet 22b, gas outlet 22c are connected to outlet area 13 respectively and position are opposite
It answers, the gas in test paper area 12 is more directly and quickly discharged from gas outlet.
During liquid to be detected is guided test paper 121 into 1 test paper area 12 of substrate by S203, may exist by
Quantity in be connected to test paper area 12, loading area 11 is excessive, causes the test paper 121 in the farther away test paper area 12 of injection port 21
Liquid to be detected is touched later compared with the test paper 121 in the closer test paper area 12 of injection port, so that each test paper in detection card
121 reaction time is inconsistent.In order to solve this problem, optionally, as shown in Figure 7 and Figure 8, detection card further includes being located at examination
The guiding thing 14 for having water suction property in paper area 12 and 11 linking area of loading area, one end of guiding thing 14 is located at loading area
11, the other end of guiding thing 14 is in contact with the test paper 121 in test paper area 12.There is the guiding thing of water suction property by being provided with
14 so that the test paper 121 being in contact with guiding thing 14 can almost absorb that guiding thing 14 absorbs from loading area 11 simultaneously to
Detection liquid simultaneously reacts therewith, to solve the problems, such as that the reaction time of test paper 121 in each test paper area 12 is inconsistent.
Optionally, as shown in Figure 7 and Figure 8, substrate 1 further includes the first fluid channel for being connected to test paper area 12 and outlet area 13
15, the width in first fluid channel 15 is less than the width of test paper 121.First fluid channel 15 can keep test paper area 12 and go out
While connection between gas area 13, certain fixed function is played to the test paper 121 in test paper area 12, prevents test paper 121 from sending out
Raw displacement.Moreover, can also be passed through after liquid to be detected is guided the test paper 121 into 1 test paper area 12 of substrate by S203
One fluid channel 15 and test paper area 12 control the liquid to be detected amount that test paper area 12 is accommodated, and in general, test paper area 12 is held
The liquid to be detected amount received is related with the relative width of the groove volume in test paper area 12 and first fluid channel 15, sets in substrate
Timing rationally controls the groove volume in test paper area 12 and the relative width in first fluid channel 15, can be in use
The liquid to be detected amount that control test paper area 12 is accommodated is preferred values.
In S204, the reaction time is preset according to project detected and the decision of the characteristic of test paper 121, generally 30s is extremely
15min.When using test paper 121 in detection device Test paper area 12, if encapsulated layer 2 and/or interior encapsulated layer 23 are transparent material
Matter then can be detected directly, if encapsulated layer 2 and/or interior encapsulated layer 23 are nontransparent material, should remove encapsulated layer 2 and/or interior envelope
The test paper 121 in test paper area 12 is detected again after dress floor 23.Therefore, a kind of to be more preferably achieved in that using transparent
Material constitutes encapsulated layer 2 and/or interior encapsulated layer 23, and such as polyester resin (Polyethylene terephthalate, PET) gathers
Methyl methacrylate (Polymeric Methyl Methacrylate, PMMA), polycarbonate (Polycarbonate, PC)
Equal materials.
In embodiments of the present invention, due to 2 package substrate 1 of encapsulated layer, it is and big to be formed first to handle encapsulated layer 2
Gas phase connection injection port 21 and gas outlet 22, due to substrate 1 be provided with the loading area 11 being directly or indirectly connected to from each other,
Test paper area 12 and outlet area 13, therefore being located at the injection port of the top of loading area 11 and gas outlet above outlet area 13 can be with
Realize the internal balance with ambient atmosphere of detection card, so as to avoid liquid to be detected during detecting card internal flow gas
To the blocking that liquid flow to be detected is moved, the water imbibition of test paper 121 itself, allows liquid to be detected to be impregnated with test paper area 12 in addition
In test paper 121, to realize to the sample-adding of test paper 121.
In detection card provided by the embodiment of the present invention, the loading area of substrate both can directly connect with the side in test paper area
It is logical, it can also be with indirect communication.The embodiment of the present invention also provides a kind of following implementation of feasible method for analyzing body fluid, is applicable in
The detection card belonging to the substrate for realizing loading area and test paper area indirect communication by fluid channel.
Fig. 9 is a kind of detection card structure top view provided in an embodiment of the present invention, as shown in figure 9,2 package substrate of encapsulated layer
1, constitute confined space.Substrate 1 includes loading area 11, the test paper area 12 for carrying test paper 121, outlet area 13；Loading area 11 with
The sample introduction side in test paper area 12 passes through 17 indirect communication of second fluid channel 16 and third fluid channel；Outlet area is another with test paper area
Side is connected to by first fluid channel 15.Wherein, second fluid channel 16 is connected to loading area 11, third fluid channel 17 with
Test paper area 12 is connected to, and third fluid channel 17 is the branch in second fluid channel 16.It is carried out using detection card as shown in Figure 9
When body fluid analysis, step is roughly the same with previous embodiment, and difference is, in S201, " processing encapsulated layer 2, forms equal and atmosphere
In the injection port being connected and gas outlet ", third fluid channel 17 be it is multiple, test paper area 12 be it is multiple；Any one third fluid
Channel 17 is connected with the sample introduction side at least one test paper area, and corresponding 13 quantity of outlet area increases, therefore need to go out at each
Gas area all outputs port 22 in 13 top, as shown in figure 9, certainly, to enhance exhaust effect, can also open above outlet area 13
More gas outlets, such as gas outlet 22a, the gas outlet 22b in Fig. 5.
In S203, in " liquid to be detected to be guided to the test paper 121 into 1 test paper area 12 of substrate ", in loading area 11
Liquid to be detected is flowed along second fluid channel 16, and further flows into branch's third fluid channel in second fluid channel 16
17, since third fluid channel 17 is connected to test paper area 12, liquid to be detected will flow into test paper area 12 along third fluid channel 17,
And it reacts with the test paper 121 in test paper area 12.While liquid to be detected flowing, the venthole 22 of 13 top of outlet area
The internal connection with ambient atmosphere of detection card is realized, avoids the blocking that the gas inside detection card flows liquid, in addition
Test paper 121 has stronger water imbibition in itself, the test paper 121 that liquid is impregnated in test paper area 12, to realize nothing
Outer power drive completes the sample-adding to multiple test paper 121 next time.
By Fig. 8 and detection shown in Fig. 9 card as it can be seen that it can be respectively used to different types of test paper sample-adding, it is suitable for be checked
Survey the composition detection of liquid different type reaction principle.The embodiment of the present invention also provides one kind can be simultaneously at least two test paper
The detection card and its application method of sample-adding.Figure 10 is a kind of detection card structure top view provided in an embodiment of the present invention, such as Figure 10
Shown, the substrate 1 for detecting card includes loading area 11, the first reaction zone A, second reaction zone B；It is reacted respectively with first in loading area 11
Area A is connected to second reaction zone B；Wherein, loading area 11 is used to add liquid to be detected to the first reaction zone A and second reaction zone B
Body；First reaction zone A is used for the composition detection of liquid to be detected first kind reaction principle；Second reaction zone B is for be detected
The composition detection of liquid Second Type reaction principle.Wherein, loading area 11 and the first reaction zone A may be constructed and phase shown in Fig. 8
Same or similar board structure realizes the composition detection of liquid to be detected first kind reaction principle, and loading area 11 and second is instead
Answer area B to may be constructed board structure same as shown in Figure 9 or similar, realize liquid to be detected Second Type reaction principle at
Sorting is surveyed, and a kind of feasible specific detection card structure is as shown in figure 11, is that a kind of board structure provided in an embodiment of the present invention is bowed
View, as shown in figure 11, referring to FIG. 10, the first reaction zone A includes that multiple test paper areas 12, outlet area 13 and loading area 11 connect
The third fluid channel 17 that logical second fluid channel 16 and at least one is connected to second fluid channel 16；Third fluid channel
17 be the branch in second fluid channel 16；Every third fluid channel 17 is connected with the sample introduction end in multiple test paper areas 12；Each
Test paper area 12 is for carrying test paper.Further, the test paper area ipsilateral with third fluid channel 17 is located at, each outlet area 13
12 sample outlet end is connected.Meanwhile second reaction zone B includes the test paper area 12 of multiple parallelly distribute ons；The examination of multiple parallelly distribute ons
Each test paper area 12 in paper area 12 is for carrying at least one test paper 121.
Method for analyzing body fluid provided by the embodiment of the present invention is equally applicable to detection card as shown in figure 11, specific implementation
Process is similar with foregoing invention embodiment, wherein gas outlet formed in S201 includes the outlet in the first reaction zone A
Mouthful, and include the gas outlet in the B of second reaction zone.In S203, liquid to be detected from injection port to loading area 11 that injected by
It is spread simultaneously to the first reaction zone A and second reaction zone B, liquid to be detected is successively logical along second fluid in the first reaction zone A
Road 16 and third fluid channel 17 flow into test paper area 12, and the first kind occurs with the test paper 121 in test paper area 12 and reacts；?
Liquid to be detected is absorbed by the guiding thing 14 of water imbibition and diffuses to the test paper 121 being connected with guiding thing in two reaction zone B, with
Second Type reaction occurs for test paper 121.
Through the foregoing embodiment, it is realized to the corresponding test paper of at least two type reaction principles using a detection card
Sample-adding, while be not required to external motive device driving, that is, simplify operation, and reduce costs.
In conclusion the embodiment of the present invention provides a kind of method for analyzing body fluid, it is applied to detection card, detection card includes base
The test paper that plate, the substrate are carried, and for package substrate and test paper with by the encapsulated layer of test paper and isolated from atmosphere, substrate packet
Loading area, test paper area and outlet area for carrying test paper are included, loading area is directly or indirectly connected to the sample introduction side in test paper area, out
Gas area is directly or indirectly connected to the sample side that goes out in test paper area, and loading area, test paper area and outlet area are recessed to be independently arranged on substrate
Slot structure, this method comprises: processing encapsulated layer, forms the injection port being connected with atmosphere and gas outlet；Injection port is located at upper
The top in sample area, gas outlet are located at the top in substrate outlet area；Liquid to be detected is injected to the loading area of substrate by injection port；
Liquid to be detected is guided into the test paper into substrate test paper area；After waiting the default reaction time, the test paper in Test paper area.
Due to encapsulating layer package substrate, encapsulated layer need to be handled first to form the injection port that is connected with atmosphere and gas outlet, by
It is provided with the loading area, test paper area and outlet area being directly or indirectly connected to from each other in substrate, therefore is located above loading area
Injection port and the gas outlet above outlet area the internal balance with ambient atmosphere of detection card may be implemented, so as to avoid
The liquid to be detected blocking that gas moves liquid flow to be detected during detecting card internal flow, the in addition water suction of test paper itself
Property, allow liquid to be detected to be impregnated with the test paper in test paper area, to realize that test paper is loaded.
Although preferred embodiments of the present invention have been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (11)
1. a kind of method for analyzing body fluid, which is characterized in that be applied to detection card, the detection card is held including substrate, the substrate
The test paper of load, and for encapsulating the substrate and the test paper with by the encapsulated layer of the test paper and isolated from atmosphere, the base
Plate includes loading area, test paper area and outlet area for carrying test paper, and the sample introduction side in the loading area and the test paper area is direct
Or indirect communication, the outlet area are directly or indirectly connected to the sample side that goes out in the test paper area, the loading area, the test paper area
It is the groove structure being independently arranged on the substrate with the outlet area, this method comprises:
The encapsulated layer is handled, the injection port being connected with atmosphere and gas outlet are formed；The injection port is located at the loading
The top in area, the gas outlet are located at the top in substrate outlet area；
Liquid to be detected is injected to the loading area of the substrate by the injection port；
The liquid to be detected is guided into the test paper into substrate test paper area；
After waiting the default reaction time, the test paper in the test paper area is detected；
The substrate is with hydrophilic substrate；
The liquid to be detected is guided into the test paper into substrate test paper area, comprising:
The liquid to be detected is guided to the test paper into substrate test paper area by substrate.
2. the method as described in claim 1, which is characterized in that by the injection port to the loading area of the substrate inject to
Before detection liquid, further includes:
It is horizontally arranged the detection card.
3. the method as described in claim 1, which is characterized in that the encapsulated layer includes interior encapsulated layer and outer package layer；It is described
Interior encapsulated layer is the transparent film layer for covering the substrate, and the region that the interior encapsulated layer corresponds to the outlet area is provided with outlet
Mouthful, the region that the interior encapsulated layer corresponds to the loading area is provided with injection port；The outer package layer is the packet of the detection card
Pack；
The encapsulated layer is handled, injection port, gas outlet is made to be connected with atmosphere, comprising:
The outer package layer is removed, the injection port on the interior encapsulated layer and the gas outlet are exposed.
4. the method as described in claim 1, which is characterized in that the encapsulated layer is the transparent film layer for covering the substrate, institute
It states encapsulated layer and the substrate constitutes confined space；
The encapsulated layer is handled, injection port, gas outlet is made to be connected with atmosphere, comprising:
Position corresponding with the loading area of the substrate is selected on the encapsulated layer and opening is set, and obtains the injection port,
And select position corresponding with the outlet area of the substrate on the encapsulated layer and opening is set, the gas outlet is obtained,
The injection port, gas outlet are connected with atmosphere.
5. method as claimed in claim 4, which is characterized in that be provided with auxiliary projection in the loading area；The auxiliary is convex
It rises for propping up the encapsulated layer；
Position corresponding with the loading area of the substrate is selected on the encapsulated layer and opening is set, and obtains the injection port,
Include:
The region close to the auxiliary projection is selected on the encapsulated layer and opening is set, and obtains the injection port.
6. method as claimed in claim 4, which is characterized in that be preset with gas outlet mark and injection port mark on the encapsulated layer
Know, the gas outlet mark is located above the outlet area, and the injection port mark is located above the loading area；
Position opening corresponding with the loading area of the substrate, obtains the injection port on the encapsulated layer, and, described
Position opening corresponding with the outlet area of the substrate, obtains the gas outlet on encapsulated layer, comprising:
Region openings on the encapsulated layer where the injection port mark are as the injection port；
Region openings on the encapsulated layer where the gas outlet mark are as the gas outlet.
7. method as claimed in claim 6, which is characterized in that the quantity of the gas outlet mark is connected to the outlet area
Test paper area quantity it is corresponding；
The position of the gas outlet mark is corresponding with the position in test paper area that the outlet area is connected to.
8. the method as described in claim 1, which is characterized in that detection card further include be located at the test paper area and it is described on
The guiding thing with water suction property of sample area linking area, one end of the guiding thing is located at the loading area, the guidance
The other end of object is in contact with the test paper in the test paper area；
The liquid to be detected is guided into the test paper into substrate test paper area, comprising:
The liquid to be detected in the loading area is guided by the guiding thing to the test paper.
9. the method as described in claim 1, which is characterized in that the substrate further includes being connected to the test paper area and the outlet
The first fluid channel in area, the width in the first fluid channel are less than the width of the test paper；
After the liquid to be detected to guide to the test paper into substrate test paper area, further includes:
The liquid to be detected amount that the test paper area is accommodated is controlled by the first fluid channel and the test paper area.
10. the method as described in claim 1, which is characterized in that the substrate further includes second be connected to the loading area
Fluid channel and the third fluid channel being connected to the test paper area；The third fluid channel is the second fluid channel
Branch；
The liquid to be detected is guided to the test paper into substrate test paper area by substrate, comprising:
By the second fluid channel, the liquid to be detected in the loading area is guided to the third fluid channel；
By the third fluid channel, the liquid to be detected is guided into the test paper into the test paper area.
11. method as claimed in claim 10, which is characterized in that the third fluid channel is multiple；The test paper area is
It is multiple；Any one third fluid channel is connected with the sample introduction side at least one test paper area；
By the third fluid channel, the liquid to be detected is guided into the test paper into the test paper area, comprising:
By the multiple third fluid channel, the liquid to be detected is separately directed to the examination in the multiple test paper area
Paper.
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| CN201710718118.1A CN109085348B (en) | 2017-08-21 | 2017-08-21 | A kind of method for analyzing body fluid |
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| CN201710718118.1A CN109085348B (en) | 2017-08-21 | 2017-08-21 | A kind of method for analyzing body fluid |
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| WO2017024271A1 (en) * | 2015-08-06 | 2017-02-09 | Lia Diagnostics, Inc. | Water dispersible assays |
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| US10648974B2 (en) * | 2014-05-28 | 2020-05-12 | Carehealth America Corporation | Ethyl glucuronide lateral flow test strips, immunoassays, devices and methods for detecting or measuring thereof |
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| CN103079704A (en) * | 2010-08-25 | 2013-05-01 | 康卡特诺英国有限公司 | Sample testing apparatus and method |
| CN202710570U (en) * | 2012-06-15 | 2013-01-30 | 北京中检葆泰生物技术有限公司 | Kit for fast detecting patulin |
| CN103424547A (en) * | 2013-08-15 | 2013-12-04 | 广州万孚生物技术股份有限公司 | Liquid detection cup and method for manufacturing same |
| CN104678108A (en) * | 2015-03-04 | 2015-06-03 | 安徽惠邦生物工程股份有限公司 | Detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase |
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| CN109085348A (en) | 2018-12-25 |
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