CN103424547A - Liquid detection cup and method for manufacturing same - Google Patents

Liquid detection cup and method for manufacturing same Download PDF

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Publication number
CN103424547A
CN103424547A CN201310357045XA CN201310357045A CN103424547A CN 103424547 A CN103424547 A CN 103424547A CN 201310357045X A CN201310357045X A CN 201310357045XA CN 201310357045 A CN201310357045 A CN 201310357045A CN 103424547 A CN103424547 A CN 103424547A
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cup
test
test paper
test strips
hole
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CN103424547B (en
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龙晖
王继华
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Guangzhou Wondfo Biotech Co Ltd
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Guangzhou Wondfo Biotech Co Ltd
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Abstract

The invention discloses a liquid detection cup and a method for manufacturing the same, and belongs to the technical field of liquid detection. The liquid detection cup comprises a cup, a test strip clamp plate and cover films; the test strip clamp plate is arranged in the cup, test strip slots used for accommodating test strips are formed in the test strip clamp plate, and the cover films cover opening surfaces of the test strip slots; through holes are formed in the cover films corresponding to the test strip slots, and the distance from the center of each through hole to the edge of the bottom of the corresponding test strip slot is 1/30-1/2 of the height of the test strip slot. The liquid detection cup and the method have the advantages that air inside the test strips can be properly released by the aid of the through holes under the condition that chromatography reaction of the test strips is assuredly normally carried out, the purpose of relieving the pressure is achieved, accordingly, a chromatography effect can be sufficiently and effectively realized, and excellent test results can be obtained.

Description

Liquid detection cup and preparation method thereof
Technical field
The present invention relates to the liquid detecting technical field, particularly relate to a kind of liquid detection cup and preparation method thereof.
Background technology
Existing detection urine cup can be used for the joint-detection of entry, wall of cup is provided with the test paper clamp, this test paper clamp is provided with the test paper slot of placing test strips, and the test strips of test use is embedded in this test paper slot uses (being that the size of test strips and the size of test paper slot are complementary).When needs detect, give by detecting urine cup the patient who needs detection, allow it fill voluntarily urine, and the patient often has little understanding to structure, the principle of test strips, when filling urine, more than very likely urine can being installed to the MAX line, cause test strips to lose efficacy, can't complete detection.
In order to address the above problem, people are through research and development, this detection urine cup has been made to improvement, simultaneously set up transparent film layer in test strips in the face of liquid, this transparent film layer is covered in the test strips surface, thereby test strips is all covered, or cover major part, only stay bottom part sample application zone and directly contact with liquid.Therefore, while in detecting cup, liquid being housed, transparent film layer gets up the test strips top seal, and only staying sample application zone can directly contact with liquid, thereby can not make ELISA test strip invalid because liquid surpasses the MAX line, and then guarantees the effective loading of test strips, normal reaction.
But we find, in the chromatography process of being everlasting, the chromatography of liquid on test strips creeped may be subject to hindering and slack-offly even stopped, and causes normally being measured.
Summary of the invention
Based on this, the object of the invention is to overcome the defect of prior art, a kind of liquid detection cup is provided, adopt this detection cup to carry out the fluid sample detection, can guarantee carrying out smoothly of chromatography process, obtain and measure preferably effect.
For achieving the above object, the present invention takes following technical scheme:
A kind of liquid detection cup, comprise cup, test paper clamp and coverlay; Described test paper clamp is placed in cup, which is provided with for the test paper slot of placing test strips, and described coverlay covers test paper slot opening surface, sealing test paper slot inner space; This through hole is located on the coverlay corresponding to the test paper slot, the 1/30-1/2 that the length of this through hole centre distance test paper slot bottom margin is test paper slot height.
The present invention finds by research, simple coating epiphragma all or the test strips that covers of major part be unfavorable for the chromatography reaction, its reason is as follows: when the test strips loading has just started, there is certain gap between test strips surface and covering hyaline membrane, in test strips, gas is pushed by extraneous liquid, part can be discharged by this gap, but expand rapidly after the sample application zone water suction, the test strips sample application zone of coverlay and imbibition fits tightly, this gap is filled disappearance soon, in test strips, gas can't be discharged from here again, so far, test strips is completely sealed.And because the reaction of test strips chromatography is to occur by capillary action, as gaseous tension in test strips is greater than the pulling force that capillary action produces, if the capillary action pulling force can't overcome the resistance of γ-ray emission in test strips, the chromatography effect of test strips will be hindered, and can't normally carry out chromatography.
And, in the process of liquid chromatography on test strips, need to get over the label district from sample application zone, then show result in results display area, this process is undertaken by capillary pulling force.And once test strips inside is closed, the unnecessary gas remained in test strips can't be discharged, in the inner certain pressure intensity that forms of test strips, in the liquid process that chromatography is creeped on test strips, need opposing to live this internal pressure, once pressure is greater than the pulling force that capillary action produces, the chromatography effect will be hindered or can't normally be carried out.From such principle, the present invention has investigated multiple release mode, and, through a large amount of experimental studies, the final selection arranges by the bottom corresponding to test strips on coverlay the purpose that through hole reaches release.
The present invention also provides a kind of liquid detection cup, comprises cup, test paper clamp, test strips and coverlay; Described test paper clamp is placed in cup, which is provided with the test paper slot, and described test strips is positioned in described test paper slot, and described coverlay covers the detection faces of test strips, the sealing test strips; Described coverlay is provided with through hole, and this through hole is located on the coverlay corresponding to the test paper slot, the 1/30-1/2 that the length of this through hole centre distance test paper slot bottom margin is test paper slot height.
In embodiment, described through hole is located at corresponding on the coverlay below the test strips label pad therein.The present invention finds after deliberation, and the setting position of through hole is also very crucial.Higher when the position of through hole, as while being arranged on the thieving paper position corresponding to the test strips sample application zone other end, when in detecting cup, liquid levels is lower than this through hole, in test strips, unnecessary gas can be discharged by this through hole, and chromatography reacts and still can normally carry out; And in cup liquid level during higher than this through hole, in a flash incipient, form a gas column between the test strips that the coating epiphragma seals and coverlay, through hole is equivalent to the opening on this gas column, and at this moment, this gas column and outside liquid form huge pressure difference, the gas column internal gas pressure is far smaller than outside liquid pressure, in order to reach inside and outside pressure equilibrium, outside liquid will enter in this gas column by this through hole under the pressure of this pressure difference, and soak rapidly the test strips that full coating epiphragma seals, cause test strips invalid.
Below through hole being located at corresponding to test strips bottom label pad, on the coverlay at (corresponding to the sample application zone place), through hole is in the bottom of test strips.Liquid is in test strips sample application zone loading process, after the sample application zone water suction, expand gradually, but also not with cover in the situation that hyaline membrane fits fully, there is gap in the test strips sample application zone with covering between hyaline membrane, in test strips, gas can be by this gap and through hole discharge; And, when sample application zone expand into fully laminating and covers hyaline membrane, gap is sealed, the gas in test strips can't be discharged from here again.When liquid is come lead to the hole site, the yet imbibition gradually of the test strips of through hole part, also do not expanding into firmly hyaline membrane of laminating fully, and in the situation that through hole is not blocked fully, in test strips, gas still can be discharged from this through hole.When in cup, liquid level is lower than the through hole position, the Exhaust Gas naked eyes are invisible; When in cup, liquid level is higher than through hole place height, liquid is discharged macroscopic bubble by through hole.When the test strips of through hole continues to expand into when coverlay is lived in laminating fully, gas can not be discharged from through hole again, and now, test strips is in complete sealing state, and test strips will be carried out chromatography by capillary force.At this moment may still have a certain amount of gas in test strips, but, after place that can exhaust by above-mentioned two places discharges, the interior pressure of test strips is moderate, the chromatography effect can be carried out smoothly.
The reasonable setting of this through hole can be assisted the discharge of gas in test strips, after the test strips at coverlay bottom margin place expands, exhausr port is blocked, unnecessary gas makes in test strips when can't be discharged from here again, gas also can be discharged by through hole, until expanding, test strips blocks through hole, thereby extended gas efflux time in test strips, reduced pressure in test strips, guaranteed that the chromatography effect normally carries out.
Therein in embodiment, the 1/6-5/6 that described coverlay bottom edge is sample application zone length on test strips apart from the length of test strips bottom margin.And the bottom edge reference position of coverlay and through hole setting position are very crucial to the quality of chromatography effect, allow coverlay cover a part of sample application zone, rather than test strips is covered fully or only started to cover by the label pad district, purpose is to make liquid that a buffer action be arranged when the test strips loading, liquid can get over labeling pad by the chromatography effect, then shows result in viewing area.As test strips is covered fully, be difficult to the loading that assures success; As coverlay only overlay marks pad district and above part, the sample application zone of steeping in liquid is oversize, and liquid may directly be broken through label pad, rushes the sample phenomenon, causes the chromatography reaction normally not carry out.
Therein in embodiment, the distance of the distance of described through hole center and test strips bottom margin and through hole center and label pad lower limb is than being 7:1-3:1.When through hole is positioned at this height, inside and outside pressure difference the best of test strips, can obtain best chromatography effect.
Therein in embodiment, the 1/3-1/2 that the diameter of described through hole or the length of side are the test strips width.As through hole is too large, liquid easily directly enters in test strips by this through hole, and through hole does not have scavenging action, is equivalent to coverlay from through hole, and through hole performs practically no function.And if the through hole setting is too little, when liquid on test strips during loading, sample application zone expands rapidly, soon through hole is sealed, the gas flow of discharging from this through hole is quite few, be unfavorable for the release of pressure in test strips, the gas that remains in test strips inside is more, is unfavorable for that on test strips, the chromatography sufficient reacting carries out.Therefore, through after experiment repeatedly, while determining the 1/3-1/2 that diameter or the length of side when through hole are the test strips width, best results.
In embodiment, described coverlay is hyaline membrane therein; Described test paper clamp circumferentially arranges along cup, and described test paper slot is located at the test paper clamp towards cup one side, and described cup is transparent cup, is convenient to observe reading result.
In embodiment, described test paper clamp is connected with cup is detachable therein.Production, easy to assembly.
In embodiment, also comprise bowl cover, described bowl cover and cup threaded engagement therein; Be provided with O-ring seal at the bottom of described bowl cover.Coordinate the use of bowl cover, can avoid detecting the problem of fluid seepage in cup.The use of O-ring seal, improved the leakproofness between cup and bowl cover.Described O-ring seal is preferably made by silica gel.Silica gel material is difficult for aging, good leak tightness, and it is inert material, can not react with the liquid detected in cup, affects testing result.And, when tested liquid need to be sent to secondary detection, this is provided with and is beneficial to sealing and preserves a liquid in cup, avoid liquid in the process of transporting owing to being inverted, tilting to leak, affect censorship, contaminated environment.
The present invention also provides a kind of method for making of liquid detection cup, comprises the following steps:
1) with the detection faces of test strips outside the test paper slot, test strips is positioned in the test paper slot on the test paper clamp;
2) be covered in above-mentioned ELISA test strip face with the coverlay that is provided with through hole, test strips is sealed in the confined space that coverlay and test paper slot form, and the position that makes described through hole is corresponding to the following part of test strips label pad;
The test strips that 3) will assemble by above-mentioned steps, coverlay and test paper clamp are put into the cup that detects cup, obtain.
The liquid detection cup made by said method, can guarantee carrying out smoothly of chromatography process, obtains and measure preferably effect.
Compared with prior art, the present invention has following beneficial effect:
Liquid detection cup of the present invention, corresponding to part below the test strips label pad, through hole is set on coverlay, the reasonable setting of this through hole can be assisted the discharge of gas in test strips, after the test strips at coverlay bottom margin place expands, the place exhausr port is blocked, make in test strips when unnecessary gas can't be discharged from here, gas also can continue to discharge by described through hole, until test strips expands, blocks through hole, thereby further reduced pressure in test strips, guaranteed that the chromatography effect normally carries out.
And the present invention designs by repetition test and verifies, finally determined the parameters such as best coverlay reference position, lead to the hole site and size, can obtain with optimum chromatography process best test result.
The liquid detection cup made by making liquid detection cup method of the present invention, can guarantee carrying out smoothly of chromatography process, obtains and measure preferably effect.
The accompanying drawing explanation
Detection cup cup, test paper clamp and cup lid structure schematic diagram that Fig. 1 is embodiment 1;
Fig. 2 is Fig. 1 pilot scale paper templet structural representation;
Fig. 3 is test paper slot floor map on Fig. 1 pilot scale paper templet;
Fig. 4 is embodiment 1 covered film structure schematic diagram;
Fig. 5 is embodiment 1 test strips structural representation;
Fig. 6 is embodiment 1 test paper clamp, test strips and coverlay assembling schematic diagram;
Fig. 7 is that Fig. 6 has assembled schematic diagram;
Fig. 8 is Fig. 7 front view;
The cup of the detection cup that Fig. 9 is embodiment 2, test paper clamp and cup lid structure schematic diagram.
Wherein: 1. cup; 2. test paper clamp; 21. test paper slot; 3. test strips; 31. sample application zone; 32. label pad district; 33. results display area; 331. detection zone; 332. control zone; 34. thieving paper; 4. coverlay; 41. through hole; 5. bowl cover; 51. O-ring seal.
Embodiment
Describe the present invention in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
A kind of liquid detection cup, as shown in Fig. 1, Fig. 6, comprise cup 1, test paper clamp 2, test strips 3 and coverlay 4; Described test paper clamp 2 is placed in cup 1, which is provided with test paper slot 21, and described test strips 3 is positioned in described test paper slot 21, as shown in Fig. 5-6, and the wide 3mm of described test strips 3, long 60mm; Sample application zone 31 and label pad district 32 be long 20mm altogether, wherein, and sample application zone 12mm, label pad district 8mm; Results display area 33 is 10mm, is provided with again detection zone 331 and control zone 332 on results display area 33; Thieving paper 34 part 22mm.Described coverlay 4 is hyaline membrane, and as shown in Figure 4, coverlay 4 is covered in the detection faces of test strips 2, the sealing test strips; And the dash area single spreading in Fig. 4, in order to paste with test paper clamp 2, coverlay 4 is adhesively fixed.Coverlay 4 bottom edge are 8mm apart from the length of test strips 3 bottom margins; Be provided with through hole 41 corresponding to part below test strips 2 label pad on described coverlay 4, as shown in Fig. 6-8, described through hole 41 centre distance label pad district 32 lower limb 2mm, this through hole 41 is circular, its diameter is 1.25mm.Be understandable that, these through hole 41 shapes also can be for square, and any shape such as triangle, as long as can be by gas.
As Figure 1-3, described test paper clamp 2 circumferentially arranges along cup 1, and described test paper slot 21 is located at test paper clamp 2 towards cup 1 one sides, and described cup is transparent cup.Be convenient to observe and reading result.
Described test paper clamp 2 is connected with cup 1 is detachable.Production, easy to assembly.Described cup 1 bottom is square.
This detection cup also comprises bowl cover 5, described bowl cover 5 and cup 1 threaded engagement; Be provided with O-ring seal 51 at the bottom of described bowl cover.Coordinate the use of bowl cover 5, can avoid detecting the problem of fluid seepage in cup.The use of O-ring seal, improved the leakproofness between cup and bowl cover.Described O-ring seal is preferably made by silica gel.Silica gel material is difficult for aging, good leak tightness, and it is inert material, can not react with the liquid detected in cup, affects testing result.And, when tested liquid need to be sent to secondary detection, this is provided with and is beneficial to sealing and preserves a liquid in cup, avoid liquid in the process of transporting owing to being inverted, tilting to leak, affect censorship, contaminated environment.
The method for making of the liquid detection cup of the present embodiment is:
1) with the detection faces of test strips outside the test paper slot, test strips is positioned in the test paper slot on the test paper clamp, fix;
2) be covered in above-mentioned ELISA test strip face with the coverlay that is provided with through hole, and at coverlay and test paper clamp contact portion gluing, in order to paste with the test paper clamp, thereby test strips is sealed in the confined space that coverlay and test paper slot form, and the position that makes described through hole is corresponding to the following part of test strips label pad;
The test strips that 3) will assemble by above-mentioned steps, coverlay and test paper clamp are put into the cup that detects cup, obtain.
Embodiment 2
As shown in Figure 9, the detection cup of the liquid detection cup of the present embodiment and embodiment 1 is basic identical, and the key distinction is:
The coverlay bottom edge is 6mm apart from the length of test strips bottom margin; Be provided with through hole corresponding to part below the test strips label pad on described coverlay, described through hole centre distance label pad district lower limb 3mm, this through-hole diameter is 1mm.
Described bottom of cup is circular.
Embodiment 3
The detection cup of the liquid detection cup of the present embodiment and embodiment 1 is basic identical, and the key distinction is:
The coverlay bottom edge is 9mm apart from the length of test strips bottom margin; Be provided with through hole corresponding to part below the test strips label pad on described coverlay, described through hole centre distance label pad district lower limb 1.5mm, this through-hole diameter is 1.5mm.
Embodiment 4
The detection cup of the liquid detection cup of the present embodiment and embodiment 1 is basic identical, and the key distinction is:
Application of sample head of district 15mm, the coverlay bottom edge is 10mm apart from the length of test strips bottom margin; Be provided with through hole corresponding to part below the test strips label pad on described coverlay, described through hole centre distance label pad district lower limb 2.5mm, this through-hole diameter is 1.4mm.
Embodiment 5 comparative experimental example
Adopt detection cup, the through hole of embodiment 1-4 to be located at the detection cup of not establishing through hole in the detection cup of thieving paper position and prior art on coverlay and carry out chromatography test.
Wherein: it is basic identical that through hole is located at the detection cup of the detection cup of thieving paper position and embodiment 1, and the key distinction is, the coverlay bottom edge is 17mm apart from the length of test strips bottom margin; Be provided with through hole corresponding to test strips thieving paper position on described coverlay, described through hole centre distance test strips tip edge 5mm.
This experiment is by being divided into 500 parts to gathering the sample come, employing specification, the ketamine test item test strips that type is identical are tested, and will detect glass interior liquid and all be filled into the results display area do not had on test strips, investigate its effect, experimental result is as shown in table 1 below.
Figure BDA00003671646500081
By the above results, can find out, use the detection cup of not establishing through hole in prior art, because the test strips internal pressure is excessive, the required loading overlong time of chromatography effect that causes the part test strips, even can't successful loading, be the tracer liquid precalculated position of can not climbing, can't make control zone produce respective change, the loading rate is lower.And through hole is located to the thieving paper part, due to liquid climb too fast, cause determinand also not have and labeling pad in respective markers thing reaction, or liquid is broken through detection zone fast, there is no time enough and the antigen/antibody reaction that is coated on detection zone, cause the sample rate of rushing of generation higher.And the detection cup of embodiment of the present invention 1-4, owing to having adopted preferably the parameters such as coverlay reference position, lead to the hole site and size, its loading rate is higher, rushes the sample rate little, detects duration moderate, and best detection effect is arranged.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (10)

1. a liquid detection cup, comprise cup, test paper clamp and coverlay; Described test paper clamp is placed in cup, which is provided with for the test paper slot of placing test strips, and described coverlay covers test paper slot opening surface; It is characterized in that: described coverlay is provided with through hole, and this through hole is located on the coverlay corresponding to the test paper slot, the 1/30-1/2 that the length of this through hole centre distance test paper slot bottom margin is test paper slot height.
2. a liquid detection cup, comprise cup, test paper clamp, test strips and coverlay; Described test paper clamp is placed in cup, which is provided with the test paper slot, and described test strips is positioned in described test paper slot, and described coverlay covers the detection faces of test strips; It is characterized in that: described coverlay is provided with through hole, and this through hole is located on the coverlay corresponding to the test paper slot, the 1/30-1/2 that the length of this through hole centre distance test paper slot bottom margin is test paper slot height.
3. liquid detection cup according to claim 2, is characterized in that, described through hole is located at corresponding on the coverlay below the test strips label pad.
4. liquid detection cup according to claim 2, is characterized in that, the 1/6-5/6 that described coverlay bottom edge is sample application zone length on test strips apart from the length of test strips bottom margin.
5. liquid detection cup according to claim 2, is characterized in that, the distance of the distance of described through hole center and test strips bottom margin and through hole center and label pad lower limb is than being 7:1-3:1.
6. liquid detection cup according to claim 2, is characterized in that, the 1/3-1/2 that the diameter of described through hole or the length of side are the test strips width.
7. liquid detection cup according to claim 2, is characterized in that, described coverlay is hyaline membrane; Described test paper clamp circumferentially arranges along cup, and described test paper slot is located at the test paper clamp towards cup one side, and described cup is transparent cup.
8. liquid detection cup according to claim 2, is characterized in that, described test paper clamp is connected with cup is detachable.
9. liquid detection cup according to claim 2, is characterized in that, also comprises bowl cover, described bowl cover and cup threaded engagement; Be provided with O-ring seal at the bottom of described bowl cover.
10. the method for making of a liquid detection cup, is characterized in that, comprises the following steps:
1) with the detection faces of test strips outside the test paper slot, test strips is positioned in the test paper slot on the test paper clamp;
2) be covered in above-mentioned ELISA test strip face with the coverlay that is provided with through hole, test strips is sealed in the confined space that coverlay and test paper slot form, and the position that makes described through hole is corresponding to the following part of test strips label pad;
The test strips that 3) will assemble by above-mentioned steps, coverlay and test paper clamp are put into the cup that detects cup, obtain.
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CN105044102B (en) * 2015-08-10 2019-02-01 杭州康永生物技术有限公司 Test strips inserted sheet, manufacturing method and detection device for detection cup
CN106645663A (en) * 2017-01-22 2017-05-10 英科新创(厦门)科技有限公司 Anti-pollution vertical tomography detection device
CN106645663B (en) * 2017-01-22 2023-07-18 英科新创(厦门)科技股份有限公司 Anti-pollution vertical chromatography detection device
CN107271334B (en) * 2017-05-25 2019-09-17 东莞长盈精密技术有限公司 Film layer liquid detecting system
CN107271334A (en) * 2017-05-25 2017-10-20 东莞长盈精密技术有限公司 Film layer liquid detecting system
CN109085159A (en) * 2017-08-21 2018-12-25 上海荒岛科技有限公司 A kind of substrate and detection card
CN109085348B (en) * 2017-08-21 2019-07-23 上海荒岛科技有限公司 A kind of method for analyzing body fluid
CN109085348A (en) * 2017-08-21 2018-12-25 上海荒岛科技有限公司 A kind of method for analyzing body fluid
CN109342673A (en) * 2018-12-14 2019-02-15 深圳市心月生物科技有限公司 A kind of cream jug
WO2021017417A1 (en) * 2019-07-30 2021-02-04 南通伊仕生物技术股份有限公司 Human chorionic gonadotropin semi-quantitative detection test paper and preparation method therefor, human chorionic gonadotropin semi-quantitative detection reagent cup, and applications thereof
WO2021042447A1 (en) * 2019-09-06 2021-03-11 杭州奥泰生物技术股份有限公司 Micropore pressure-equalizing anti-capillary fast-sampling fluid test device

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