CN108776217A - A kind of full instrument of immune colloid gold - Google Patents
A kind of full instrument of immune colloid gold Download PDFInfo
- Publication number
- CN108776217A CN108776217A CN201811016700.4A CN201811016700A CN108776217A CN 108776217 A CN108776217 A CN 108776217A CN 201811016700 A CN201811016700 A CN 201811016700A CN 108776217 A CN108776217 A CN 108776217A
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- kit
- sample
- master board
- scanning
- interface screen
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 109
- 239000010931 gold Substances 0.000 title claims abstract description 52
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 52
- 239000000084 colloidal system Substances 0.000 title claims abstract description 50
- 230000006698 induction Effects 0.000 claims abstract description 31
- 238000011534 incubation Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000012937 correction Methods 0.000 claims abstract description 5
- 230000003760 hair shine Effects 0.000 claims abstract description 5
- 238000001514 detection method Methods 0.000 claims description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims description 19
- 239000000020 Nitrocellulose Substances 0.000 claims description 11
- 229920001220 nitrocellulos Polymers 0.000 claims description 11
- 239000003292 glue Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 2
- 238000012545 processing Methods 0.000 description 14
- 238000005070 sampling Methods 0.000 description 9
- 238000010586 diagram Methods 0.000 description 7
- 239000013078 crystal Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 230000002093 peripheral effect Effects 0.000 description 4
- 230000005611 electricity Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- WYROLENTHWJFLR-ACLDMZEESA-N queuine Chemical compound C1=2C(=O)NC(N)=NC=2NC=C1CN[C@H]1C=C[C@H](O)[C@@H]1O WYROLENTHWJFLR-ACLDMZEESA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of full instruments of immune colloid gold, including a babinet, kit pallet, kit position induction device are provided in babinet, two-dimensional code scanning device, it is loaded sensing device, temperature-adjusting device, light source scanning photographic device, scanning driving device, master board, control interface screen, power switch and supply unit；Kit pallet places colloidal gold kit；It is loaded the addition of sensing device induction sample, master board judges that self-clocking when addition reaches predetermined amount, temperature-adjusting device induction ambient temperature are simultaneously adjusted to set temperature；Scanning driving device control light source scanning photographic device shines and scans acquisition colour developing result；Result data is transmitted to control interface screen display by master board to generating result data after colour developing interpretation of result, correction process and storing；By automatically controlling incubation time and built-in temperature, solve the problems, such as it is existing need to be incubated outside machine, manual operation error is easy to cause result error.
Description
Technical field
The present invention relates to reagent detection technique field, more particularly to a kind of full instrument of immune colloid gold.
Background technology
LED light source scanning technique is very universal for the full quantitative detection field of colloidal gold.Scanning means and detection zone it
Between lack comprehensive matching, this is because there are sweep span, width, travel speed, be protected from light, the factors such as reflective influence, cause
The problem of detection background and the lack of resolution of signal.The inadequate problem of the processing function of additional software, leads to last inspection
Survey the precision of result and the deficiency of sensitivity.
For the prior art using special LED light as light source, the comprehensive matching detection window of device of scan camera shooting system is right
The object to be checked colour developing degree and background of different colloid gold labels carry out comprehensive capture, and by powerful software to background and letter
Number operation is processed, full quantitative analysis is carried out to object to be checked according to built-in fitted calibration curve and is marked with the dense of corresponding determinand
Degree.But due to being single channel design, a kit can only be once read, it is limited on flux；And it needs to incubate outside machine when big flux
It educates, the error of manual operation is easy to cause the deviation of result.Additionally need to manually advance and kit and exit kit, completely according to
The manual operation for relying client, can not achieve intelligent propulsion.
Thus the prior art could be improved and improve.
Invention content
Place in view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of immune colloid golds quantitatively to examine entirely
Instrument is surveyed, quantitatively detection needs the error of incubation outside machine, manual operation to be easy to cause the deviation of result entirely to solve existing colloidal gold
The problem of.
In order to achieve the above object, this invention takes following technical schemes：
A kind of full instrument of immune colloid gold, including a babinet, wherein kit pallet, examination are provided in the babinet
Agent box position sensing device, two-dimensional code scanning device, sample-adding sensing device, temperature-adjusting device, light source scanning photographic device are swept
Retouch driving device, master board, control interface screen, power switch and supply unit；The sample-adding sensing device, temperature adjust dress
It sets and master board is assembled into one and connected with kit pallet, kit position induction device drives with two-dimensional code scanning device
Connection, light source scanning photographic device connect scanning driving device and master board, and master board connects scanning driving device, reagent
Box position sensing device, two-dimensional code scanning device, control interface screen, power switch and supply unit；
The kit pallet is for placing colloidal gold kit to be measured；Kit position induction device incudes colloid gold reagent
When box reaches the setting position of kit tray bottom, the information of driving two-dimensional code scanning device identification colloidal gold kit adds
Sample sensing device incudes the addition of sample, and master board judges self-clocking when the addition reaches predetermined amount, temperature tune
Regulating device incudes ambient temperature and is adjusted to the set temperature of colloidal gold kit；The scanning driving device controls light source
Scan camera shooting device shines and scans the colour developing result obtained on colloidal gold kit；Master board carries out the colour developing result
Result data is generated after analysis, correction process and is stored, and result data is transmitted to control interface screen display；The power supply is opened
The keying of the down state control supply unit of pass, powers to the full instrument of immune colloid gold when supply unit starts.
In the full instrument of the immune colloid gold, the side in babinet, light source is arranged in the kit pallet
Scan camera shooting device is disposed adjacent with kit position induction device, two-dimensional code scanning device and is located at the upper of kit pallet
Side, scanning driving device are arranged in one end of babinet and adjacent with light source scanning photographic device, and master board is located at light source scanning
The other side of photographic device, control interface screen are arranged in the upper surface of babinet and are placed in the top of master board, sample-adding induction dress
Install in kit pallet and opposite with the sample-adding bottom of window of colloidal gold kit, temperature-adjusting device and colloidal gold
The bottom of the detection window of kit is opposite.
In the full instrument of the immune colloid gold, the kit position induction device is infrared electro induction dress
It sets, judges whether colloidal gold kit is put into and incudes whether colloidal gold kit is advanced into babinet by infrared electro
Setting position.
In the full instrument of the immune colloid gold, the information of acquisition is imported master control by the two-dimensional code scanning device
In making sheet；Master board judges whether there is the chemical examination table of identical information according to the information：If so, the behaviour that then master board will prestore
It coaches and is transmitted to control interface screen display；If no, the master board prompt message that prestores of output is to control interface screen
Upper display.
In the full instrument of the immune colloid gold, whether the master board also judges the date of manufacture in information
It is expired, it is to export the warning message to prestore to control interface screen display, stopping continues sample-adding and uses.
In the full instrument of the immune colloid gold, the sample-adding sensing device is using capacitive sensing sample-adding window
Sample-adding operates, and capacitance generates corresponding variation during sample-adding；Sample-adding does not start self-clocking, control mainboard output when insufficient
Continuous plus information to control interface screen shows that control mainboard detection capacitance starts timing and be shown in control automatically when reaching threshold value
Interface screen is incubation time and result read access time.
In the full instrument of the immune colloid gold, a reagent strip, the examination are placed in the colloidal gold kit
Agent item includes the support pad of bottom, the nitrocellulose filter being located in the support pad, and the one of the nitrocellulose filter
End top stacking connection water absorption pad, the other end top stacking connection conjugates gasket of nitrocellulose filter, the conjugates pad
One end of the top stacking connection sample gasket of piece, the other end top of the sample gasket are equipped with fixing glue, and the nitric acid is fine
The intermediate position of the plain film of dimension is equipped with one or two p-wires and a control line.
In the full instrument of the immune colloid gold, further include for carry out the USB interface of data input and output and
Network interface, the USB interface and network interface are disposed adjacent the other end and exposing in babinet.
Further include a built-in printer in the full instrument of the immune colloid gold, the built-in printer connection
Control interface screen is used for the result data of print control interface screen display.
In the full instrument of the immune colloid gold, in the light source scanning photographic device, light path and LED light are imaged
Angle between source is 45 °, and camera shooting clarity is by turntable driving speed control.
Compared to the prior art, the full instrument of immune colloid gold provided by the invention, including a babinet, the babinet
It is inside provided with kit pallet, kit position induction device, two-dimensional code scanning device, sample-adding sensing device, temperature adjust dress
It sets, light source scanning photographic device, scanning driving device, master board, control interface screen, power switch and supply unit；It is described
Sample-adding sensing device, temperature-adjusting device and kit pallet are assembled into one and connect master board, kit location sensitive
Device is drivingly connected with two-dimensional code scanning device, and light source scanning photographic device connects scanning driving device and master board, master control
Making sheet connect scanning driving device, kit position induction device, two-dimensional code scanning device, control interface screen, power switch and
Supply unit；The kit pallet is for placing colloidal gold kit to be measured；Kit position induction device incudes colloid
When gold reagent box reaches the setting position of kit tray bottom, the letter of driving two-dimensional code scanning device identification colloidal gold kit
Breath, the addition of sample-adding sensing device induction sample, master board judge self-clocking when the addition reaches predetermined amount, temperature
Degree regulating device induction ambient temperature and the set temperature for being adjusted to colloidal gold kit；The scanning driving device control
Light source scanning photographic device shines and scans the colour developing result obtained on colloidal gold kit；Master board is to the colour developing result
It is analyzed, result data is generated after correction process and is stored, result data is transmitted to control interface screen display；The electricity
The keying of the down state control supply unit of source switch, supplies the full instrument of immune colloid gold when supply unit starts
Electricity.By automatically controlling incubation time and built-in temperature, solving existing colloidal gold, quantitatively detection needs machine to be incubated outside, is artificial entirely
The error of operation is easy to cause the problem of deviation of result.
Description of the drawings
Fig. 1 is the structural schematic diagram that the full instrument of immune colloid gold provided by the invention places colloidal gold kit.
Fig. 2 is structural schematic diagram when the full instrument of immune colloid gold provided by the invention is vacant.
Fig. 3 is the structure chart of the full instrument of immune colloid gold provided by the invention.
Fig. 4 is the structural schematic diagram of reagent strip in the full instrument of immune colloid gold provided by the invention.
Fig. 5 is the structural representation of the light source scanning photographic device in the full instrument of immune colloid gold provided by the invention
Figure.
Fig. 6 is the side view of camera part in light source scanning photographic device provided by the invention.
Fig. 7 is the structural schematic diagram of sweep test in two-dimensional code scanning device provided by the invention.
Fig. 8 is the circuit diagram of master board in the full instrument of immune colloid gold provided by the invention.
Fig. 9 is the circuit diagram of two-dimensional code scanning device in the full instrument of immune colloid gold provided by the invention.
Figure 10 is the circuit diagram that sensing device is loaded in the full instrument of immune colloid gold provided by the invention.
Specific implementation mode
The present invention provides a kind of full instrument of immune colloid gold, is suitable for medical diagnosis technical field, and in particular to
The full quantitative detection system of colloid gold reagent.To make the purpose of the present invention, technical solution and effect clearer, clear and definite, below
Developing simultaneously with reference to attached drawing, the present invention is described in more detail for embodiment.It should be appreciated that specific embodiment described herein is only used
To explain the present invention, it is not intended to limit the present invention.
Please refer to Fig. 1, Fig. 2 and Fig. 3, the full instrument of immune colloid gold provided in an embodiment of the present invention includes one
Babinet is provided with kit pallet A1, sample-adding sensing device A2, temperature-adjusting device A3, reagent box position sense in the babinet
Answer device B1, two-dimensional code scanning device B2, light source scanning photographic device C1, scanning driving device C2, master board D, control circle
Face F, power switch H1 and supply unit H2（Built-in rechargeable battery H3 or setting external power supply, supply unit H2 can be two
Switch between person and uses）；The kit position induction device B1 is drivingly connected with two-dimensional code scanning device B2, and the two is controlled by
Master board D；Sample-adding sensing device A2, temperature-adjusting device A3 and kit pallet A1 are assembled into one, and are controlled by main control
Plate D；Light source scanning photographic device C1 connection scanning driving device C2 and master board D, master board D connection turntable drivings dress
Set C2, control interface screen F, power switch H1 and supply unit H2（Master board D is electrically connected with built-in rechargeable battery）.
The light source scanning photographic device C1 is used to scan the object on kit pallet A1, then, can in specific setting
By the kit pallet A1（Including being loaded sensing device A2 and temperature-adjusting device A3）The side being arranged in babinet, light source
Scan camera shooting device C1（Close to body side）With kit position induction device B1（Positioned at box house）, two-dimensional code scanning dress
The top that B2 is disposed adjacent and is located at kit pallet A1 is set, scanning driving device C2 is arranged in one end of babinet and is swept with light source
Retouch that photographic device C1 is adjacent, master board D is located at the other side of light source scanning photographic device C1, and control interface screen F is arranged in case
The upper surface of body and the top for being placed in master board D.
The kit pallet A1 is arranged for placing colloidal gold kit M to be measured, sample-adding sensing device A2 in reagent
It is in box pallet and opposite with the sample-adding bottom of window of colloidal gold kit M, temperature-adjusting device A3 and colloidal gold kit M's
The bottom of detection window is opposite.Kit position induction device B1 is arranged in kit pallet A1 close to one end of babinet, is used for
Incude the disengaging of colloidal gold kit M on kit pallet A1（Colloidal gold kit M can specifically be incuded and be inserted into kit pallet A1
The setting position of bottom）.It is opposite with sample-adding window on kit pallet A1 to be loaded sensing device A2 settings（As being loaded window
Underface）Position, for incude the addition for being loaded sample in window N.Temperature-adjusting device A3 is arranged in kit pallet
A1 is upper opposite with detection window（Such as the underface of detection window）Position, for the environment temperature around incuding and be adjusted to glue
The set temperature of body gold reagent box M.
In the specific implementation, control kit pallet A1 is advanced into box house, kit when colloidal gold kit M is put into
Position induction device B1 incudes the position of colloidal gold kit M, senses that colloidal gold kit M is inserted into kit support
It when the setting position of the bottoms disk A1, then identifies that colloidal gold kit M is normally put into, drives two-dimensional code scanning device B2 to glue at this time
The Quick Response Code P being arranged on one end of body gold reagent box M is scanned, to obtain the information of colloidal gold kit M.Sample-adding induction dress
The addition of induction sample is set, master board judges self-clocking when the addition reaches predetermined amount.Temperature-adjusting device A3
Current environment temperature is adjusted into trip temperature according to set temperature, the environment temperature around colloidal gold kit M is made to meet glue
The optimum temperature being incubated needed for body gold reagent box M（That is set temperature）.The scanning driving device C2 controls light source scanning camera shooting
Device C1 shines, and driving light source scanning photographic device C1 is scanned and obtained the colour developing of result display window O on colloidal gold kit M
As a result；Master board D analyzes the colour developing result, result data is generated after correction process and is stored, by result data
It is transmitted on control interface screen F and shows.The keying of the down state control supply unit H2 of the power switch H1, supply unit
It powers to the full instrument of immune colloid gold when H2 starts（Arrow in Fig. 1 indicates the power supply to other devices）, specific right
It is loaded sensing device A2, temperature-adjusting device A3, kit position induction device B1, two-dimensional code scanning device B2, light source scanning
Photographic device C1, scanning driving device C2, master board D and the F power supplies of control interface screen.
Its position is detected automatically when detecting colloidal gold kit M and being put by kit position induction device B1, and is led to
Cross control interface screen F and instruct guest operation, wait for sample-adding finishing, by be loaded sensing device A2 self-clockings and read as a result, from
And solve the problems, such as that quantitatively detection needs to manually advance kit and causes operation not intelligent existing colloidal gold entirely.The present embodiment
In, infrared electro sensing device can be used in the kit position induction device B1, judges colloid gold reagent by infrared electro
Whether box M is put into and incudes kit（The present embodiment is colloidal gold kit M）Whether setting position in babinet is advanced into,
Ensure that each colloidal gold kit M is inserted into the position consistency of babinet, it is ensured that the scan position with light source scanning photographic device C1
Accurate corresponding to read accurate result, correct information is arrived in scanning.
In further embodiment, the sample-adding sensing device A2 incudes the sample-adding behaviour of sample-adding window using capacitive sensing mode
Make, capacitance generates corresponding variation during sample-adding.Sample-adding cannot start self-clocking when insufficient, control mainboard D outputs are continuous
Information is added to show that, to prompt client to continue sample-adding until reaching predetermined amount, control mainboard D detects capacitance to control interface screen F
Output stops when reaching threshold value plus information show to control interface screen F, to prompt client to stop being loaded.Control mainboard D control sample-addings
The incubation time of colloidal gold kit M and result read access time afterwards.
In further embodiment, the product of colloidal gold kit M is loaded in the Quick Response Code P of the colloidal gold kit M
The relevant informations such as project, date of manufacture, lot number, the term of validity.The information of acquisition is imported master board by two-dimensional code scanning device B2
In D.Master board D judges whether there is the chemical examination table of identical information according to the information：If so, the behaviour that then master board D will prestore
It coaches to be transmitted on control interface screen F and show, to instruct client to be loaded, later fill in result data to the correlation of chemical examination table
Xiang Zhong；If being shown in the prompt message to control interface screen F that no, master board D outputs prestore, to prompt customer first to enter
The chemical examination table of this batch.Meanwhile master board also judges whether the date of manufacture in Quick Response Code P information is expired, is that then output is pre-
Subsequent colour developing interpretation of result is shown and stopped in the warning message deposited to control interface screen F, can prompt client's colloidal gold
Kit M is expired, and has prevented the use of any expired colloidal gold kit M.
In the present embodiment, as shown in figure 4, placing a reagent strip in the colloidal gold kit M, the reagent strip includes bottom
The support pad 1 in portion, the nitrocellulose filter 3 being located in the support pad 1, one end top of the nitrocellulose filter 3
Stacking connection water absorption pad 2, the other end top stacking connection conjugates gasket 4 of nitrocellulose filter 3, the conjugates gasket 4
Top stacking connection sample gasket 5 one end, the sample gasket 5（The present embodiment is Whole Blood Filtration gasket）The other end on
Side is equipped with fixing glue 6（Double faced adhesive tape or one side glue）.The intermediate position of the nitrocellulose filter 3 is equipped with p-wire 7（One or two
Item）With a control line 8.
In further embodiment, the full instrument of immune colloid gold further includes for carrying out data input and output
USB interface E1 and network interface E2（Model RJ-45）；The USB interface E1 and network interface E2 be disposed adjacent babinet the other end simultaneously
Expose, is all connected with master board D.USB interface E1 and network interface E2 are separately connected corresponding peripheral hardware, could be used for exporting peripheral hardware
The data such as chemical examination table, software upgrade procedure, patient information are directed into master board D, and by result data（Or the detection of patient
Data）It spreads out of to corresponding peripheral hardware.
In further embodiment, the full instrument of immune colloid gold further includes a built-in printer G, and setting exists
The other side in babinet is simultaneously adjacent with power switch H1.Built-in printer G connection control interface screen F are used for print control interface
The result data shown on screen F, to facilitate user to understand testing result.
In the present embodiment, the design of light source scanning photographic device C1 matches setting with colloidal gold kit to be measured.In order to
Increase the clarity and precision of camera system, the present embodiment is to the height according to the result display window O of colloidal gold kit M
Degree, width, length are designed and adjust, the factors pair such as leakage light and shade reflective to avoid to light source scanning photographic device C1
The influence of results acquisition.The bevel angle at the detection window of colloidal gold kit M close to Quick Response Code end is 45° angle and the photograph of LED
Mingguang City is parallel, so as to avoid the formation of shade；And be 85 ° of angles close to the detection window inclined-plane at sample-adding end, it avoids reflective to sweeping
Retouch the influence of result.
Also referring to Fig. 5, Fig. 6 and Fig. 7, the light source in light source scanning photographic device C1 uses LED light source, passes through lens
It focuses.LED light source K2 after the focusing is with 45° angle irradiating and detecting window（That is result display window O）, angle with detection window
The inclined-plane of mouth O1 is parallel.The inclined design at the water absorption pad end based on detection window is also 45 °, with light ray parallel, detection window side
Edge shadow-free forms generated mistake and reads.Clarity is imaged by turntable driving speed control, camera shooting light path K 1 and LED light
Angle α between the K2 of source is at 45 °, collects scanning signal with detection window shape vertical angle in 90 °, avoids LED illumination light source
Interference.The LED light source and camera are tightly attached to the edge of result display window O peripheral plastic planes simultaneously, avoid outside
Light source enters interference caused by result display window.Since signal capture is special（Shadow-free, no outer light source enter）, clear,
Accurate positioning, additional software analyzing processing work(is powerful, to realize colloid gold reagent quantitative high precision and highly sensitive entirely
The performance of degree.Camera system is the precision-matched of scanning means and colloidal gold kit M structure, not by external interference, moreover it is possible to will
This application of structural design quantifies in the semi-automatic detector of multichannel and automatic detection system entirely to colloidal gold.Quick Response Code P's
Reading is then the reflection by eyeglass L by Quick Response Code identifying system R（Corresponding two-dimensional code scanning device B2）It is identified.
Also referring to Fig. 8 and Fig. 9, main chip U0 is provided in the master board（Model JZ4770）,
13rd foot to the 36th foot is by a FPC connector FB connection control interface screen, specially：The PC0/LCD_ of main chip U0
B0/LCD_REV feet, PC1/LCD_B1/LCD_PS feet, PC2/LCD_B2 feet, PC3/LCD_B3 feet, PC4/LCD_B4 feet, PC5/
LCD_B5 feet, PC6/LCD_B6 feet, PC7/LCD_B7 feet, PC10/LCD_G0/LCD_SPL feet, PC11/LCD_G1 feet, PC12/
LCD_G2 feet, PC13/LCD_G3 feet, PC14/LCD_G4 feet, PC15/LCD_G5 feet, PC16/LCD_G6 feet, PC17/LCD_G7
Foot, PC20/LCD_R0/LCD_CLS feet, PC21/LCD_R2 feet, PC22/LCD_R2 feet, PC23/LCD_R3 feet, PC24/LCD_
R4 feet, PC25/LCD_R5 feet, PC26/LCD_R6 feet, PC27/LCD_R7 feet respectively with the 1st foot of FPC connector FB, the 2nd foot,
3rd foot, the 4th foot, the 5th foot, the 6th foot, the 7th foot, the 8th foot, the 9th foot, the 10th foot, the 11st foot, the 12nd foot, the 13rd foot, the 14th foot,
15th foot, the 16th foot, the 17th foot, the 18th foot, the 19th foot, the 20th foot, the 21st foot, the 22nd foot, the 23rd foot, the 24th one-to-one company of foot
It connects.The 25th foot of FPC connector FB, the 26th foot, the 27th foot, the 28th foot, the 29th foot, the 30th foot, the 31st foot, the 32nd foot, the 33rd
Foot, the 34th foot, the 35th foot, the 36th foot, the 37th foot, the 38th foot, the 39th foot, the 40th foot, the 41st foot, the 42nd foot, the 43rd foot, the 44th
Foot, the 45th foot, the 46th foot, the 47th foot, the 48th foot are all connected with control interface screen.
The two-dimensional code scanning device B2 includes first resistor R1, scanning lens chip U1（Model W25X40）And processing
Chip U2（Model STM32F101C8t6）；The scanning lens chip U1'sThe PA3 feet of foot connection processing chip U2 are gone back
Pass through first resistor R1 the first power ends of connection（+3.3V）；The SO feet of scanning lens chip U1, SI feet, SCK feet respectively with processing
The PA6 feet of chip U2, PA7 feet, the one-to-one connection of PA5 feet；Scanning lens chip U1's、It is all connected with VCC feet
One power end（+3.3V）, the GND feet ground connection of scanning lens chip U1；The PB5 feet connection main chip U0's of processing chip U2
The PB6 feet of PB0/SA0 (CL) foot, processing chip U2 are all connected with the PB2/SA2 feet of main chip U0, the VBAT of processing chip U2
Foot, VDDA feet, VDD feet are all connected with the first power end（+3.3V）, the VSSA feet of processing chip U2, PA4 feet, PB2 feet, VSS feet,
BOOT0 feet are grounded.
The processing chip U2 output enable signals SPI_CS starts scanning lens chip U1, when exporting clock signal SCK
Scanning lens chip U1 starts to scan, by the scanning information of acquisition（SO and SI）After the U2 processing of input processing chip, output information
（STM_TXD and STM_RXD）Into main chip U0.Algorithm software built in main chip U0 judges according to the information
The no chemical examination table for having identical information：If so, then the Operating Guideline to prestore is passed through signal wire by main chip U0（LCD_B0~
LCD_B7 and LCD_R0 ~ LCD_R7）It is transmitted to control interface screen display；If no, main chip U0 passes through signal wire
（LCD_B0 ~ LCD_B7 and LCD_R0 ~ LCD_R7）The prompt message that prestores is exported to control interface screen display.
The main chip U0 also judges whether the date of manufacture in information is expired, is, by passing through signal wire
（LCD_B0 ~ LCD_B7 and LCD_R0 ~ LCD_R7）The warning message to prestore is exported to control interface screen display, stops continuing
Sample-adding uses.
Also referring to Fig. 8 and Figure 10, the sample-adding sensing device includes the first capacitance C1, the second capacitance C2, third electricity
Hold C3, the 4th capacitance C4, the 5th capacitance C5, the 6th capacitance C6, inductance L, phase inverter UD（Model SN74HC05）, triode Q,
Electrode CS, amplifier UA（Model TLC272）, second resistance R2,3rd resistor R3, the 4th resistance R4, the 5th resistance R5,
Six resistance R6, the 7th resistance R7, the 8th resistance R8, sampling A/D chip U3（Model STC12C5630AD）With crystal oscillator Y；Described first
The input terminal of one end and phase inverter UD of one end connection inductance L of capacitance C1, the other end of the first capacitance C1 connect the second capacitance
The other end and ground of C2, one end of the other end and second resistance R2 of one end connection inductance L of the second capacitance C2；Second resistance R2
The output end of other end connection phase inverter UD, one end of 3rd resistor R3, the 4th resistance R4 one end and triode Q base
Pole, the other end and ground of the 4th resistance R4 of emitter connection of triode Q, one end of the collector connection electrode CS of triode Q,
One end of one end and the 6th resistance R6 of the 5th resistance R5, the other end of the other end connection 3rd resistor R3 of the 5th resistance R5 and
Second source end（VCC）, the in-phase input end of one end and amplifier UA of the other end connection third capacitance C3 of the 6th resistance R6,
The other end of electrode CS and the other end of third capacitance C3 are grounded, and the inverting input of amplifier UA connects the 7th resistance R7's
The other end of one end of one end and the 8th resistance R8, the 7th resistance R7 passes through the 4th capacitance C4 ground connection, the output end of amplifier UA
The other end of the 8th resistance R8 and the P2.5 feet of sampling A/D chip U3 are connected, the XTAL2 feet of sampling A/D chip U3 connect the 5th capacitance C5
One end and crystal oscillator Y the 1st foot, the XTAL1 feet of sampling A/D chip U3 connect the 3rd foot of one end and crystal oscillator Y of the 6th capacitance C6；The
The 4th foot of other end connection crystal oscillator Y of five capacitance C5, the other end and ground of the 2nd foot of crystal oscillator Y, the 6th capacitance C6；Sampling A/D chip
The PA25/CS5_N feet of the P3.0/RXD feet connection main chip U0 of U3, the P3.1/TXD feet of sampling A/D chip U3 connect main system
The PA24/CS4_N feet of chip U0.
The sample-adding sensing device passes through the first capacitance C1 and the second capacitance C2（Capacitance is 1000pF）And inductance
Value is the inductance L of 1MH, generates the harmonic high frequency vibration wave of a 500KHZ, this harmonic high frequency vibration wave accesses electrode CS1.When there is outer liquid
When body is loaded, the capacitance at the both ends electrode CS1 generates variation, and the capacitance of variation causes the frequency of the harmonic high frequency vibration wave of 500KHZ to occur
Variation, so as to cause resonance end（The in-phase input end of amplifier UA+）Voltage change, this voltage signal is after amplification
It exports amplified signal SAMPLE_DET and carries out sampling processing, the induction that sampling A/D chip U3 obtains final process to sampling A/D chip U3
Data（STC_RXD and STC_TXD）It is transferred to main chip U0 processing.During sample-adding, main chip U0 is according to induction
Data（STC_RXD and STC_TXD）Judge not start self-clocking when sample-adding is insufficient（Built-in timer）, main chip U0 roots
According to sensed data（STC_RXD and STC_TXD）Detection capacitance starts timing automatically when reaching threshold value, and passes through signal wire
（LCD_B0 ~ LCD_B7 and LCD_R0 ~ LCD_R7）Incubation time and result read access time are shown in control interface screen, or are started
The countdown of incubation time, and be displayed on the screen.
It is to be appreciated that main chip U0 only lists each pin related to the present embodiment and its connection relation,
Other each pins unrelated with the present embodiment and its connection relation are the prior art（It can be arranged according to actual demand）, in Fig. 8
It is not shown.Above-mentioned each circuit may be provided on a circuit board, or is arranged in various boards according to actual demand.It is above-mentioned
Each circuit further includes other devices, other signals of other pins also input and output of each chip, the present embodiment only illustrate with it is immune
The relevant circuit structure of the improved function of the full instrument of colloidal gold, other devices and signal are the prior art, or according to
In addition actual functional capability demand is arranged, be not described further to it herein.
In conclusion the full instrument of immune colloid gold provided by the invention, is existed by kit position induction device
Detection colloidal gold kit starts instrumentation control system when being put into, including the prompt that control interface screen is loaded client, and incudes
Sample-adding starts self-clocking when reaching predetermined amount, the optimum temperature being incubated needed for reaching is adjusted by temperature, by automatically controlling
Incubation time and built-in temperature, solving existing colloidal gold, quantitatively detection needs the error of incubation outside machine, manual operation to be easy entirely
The problem of leading to the deviation of result.By the way that the angle α imaged in light source scanning photographic device between light path and LED light source is arranged
It it is 45 °, the inclined-plane at the water absorption pad end of detection window is set as 45 °, not by its edge when camera scanning result display window
The interference of shade and ambient light, to improve the clarity and precision of camera system.
The division of above-mentioned function module, can be as needed by above-mentioned function only to for example, in practical applications
Distribution is completed by different function modules, that is, different function modules is divided into, to complete all or part of foregoing description
Function.
It, can according to the technique and scheme of the present invention and its hair it is understood that for those of ordinary skills
Bright design is subject to equivalent substitution or change, and all these changes or replacement should all belong to the guarantor of appended claims of the invention
Protect range.
Claims (10)
1. a kind of full instrument of immune colloid gold, including a babinet, which is characterized in that be provided with kit in the babinet
Pallet, kit position induction device, two-dimensional code scanning device, sample-adding sensing device, temperature-adjusting device, light source scanning camera shooting
Device, scanning driving device, master board, control interface screen, power switch and supply unit；The sample-adding sensing device, temperature
Degree regulating device and kit pallet are assembled into one and connect master board, kit position induction device and two-dimensional code scanning
Device is drivingly connected, and light source scanning photographic device connects scanning driving device and master board, and master board connects turntable driving
Device, kit position induction device, two-dimensional code scanning device, control interface screen, power switch and supply unit；
The kit pallet is for placing colloidal gold kit to be measured；Kit position induction device incudes colloid gold reagent
When box reaches the setting position of kit tray bottom, the information of driving two-dimensional code scanning device identification colloidal gold kit adds
Sample sensing device incudes the addition of sample, and master board judges self-clocking when the addition reaches predetermined amount, temperature tune
Regulating device incudes ambient temperature and is adjusted to the set temperature of colloidal gold kit；The scanning driving device controls light source
Scan camera shooting device shines and scans the colour developing result obtained on colloidal gold kit；Master board carries out the colour developing result
Result data is generated after analysis, correction process and is stored, and result data is transmitted to control interface screen display；The power supply is opened
The keying of the down state control supply unit of pass, powers to the full instrument of immune colloid gold when supply unit starts.
2. the full instrument of immune colloid gold according to claim 1, which is characterized in that the kit pallet setting
Side in babinet, light source scanning photographic device are disposed adjacent simultaneously with kit position induction device, two-dimensional code scanning device
Positioned at the top of kit pallet, scanning driving device is arranged in one end of babinet and, master adjacent with light source scanning photographic device
Control panel is located at the other side of light source scanning photographic device, and control interface screen is arranged in the upper surface of babinet and is placed in master board
Top, sample-adding sensing device is arranged in kit pallet and opposite with the sample-adding bottom of window of colloidal gold kit, warm
It is opposite with the bottom of the detection window of colloidal gold kit to spend regulating device.
3. the full instrument of immune colloid gold according to claim 1, which is characterized in that the kit location sensitive
Device is infrared electro sensing device, judges whether colloidal gold kit is put into and incudes colloidal gold examination by infrared electro
Whether agent box is advanced into the setting position in babinet.
4. the full instrument of immune colloid gold according to claim 1, which is characterized in that the two-dimensional code scanning device
The information of acquisition is imported in master board；Master board judges whether there is the chemical examination table of identical information according to the information：If so,
Then the Operating Guideline to prestore is transmitted to control interface screen display by master board；If no, master board output prestores
Prompt message is to control interface screen display.
5. the full instrument of immune colloid gold according to claim 4, which is characterized in that the master board also judges
Whether the date of manufacture in information is expired, is to export the warning message to prestore to control interface screen display, stopping continues to add
Sample uses.
6. the full instrument of immune colloid gold according to claim 3, which is characterized in that the sample-adding sensing device is adopted
The sample-adding operation of window is loaded with capacitive sensing, capacitance generates corresponding variation during sample-adding；Sample-adding does not start when insufficient
Self-clocking, control mainboard output is continuous plus information to control interface screen show, when control mainboard detection capacitance reaches threshold value certainly
It is dynamic to start timing and be shown in control interface screen as incubation time and result read access time.
7. the full instrument of immune colloid gold according to claim 4, which is characterized in that in the colloidal gold kit
A reagent strip is placed, the reagent strip includes the support pad of bottom, the nitrocellulose filter being located in the support pad, institute
Stacking connection water absorption pad above one end of nitrocellulose filter is stated, connection conjugates are laminated above the other end of nitrocellulose filter
Gasket, one end of the top stacking connection sample gasket of the conjugates gasket, the other end top of the sample gasket is equipped with
Fixing glue, the intermediate position of the nitrocellulose filter are equipped with one or two p-wires and a control line.
8. the full instrument of immune colloid gold according to claim 1, which is characterized in that further include for carrying out data
The USB interface and network interface of input and output, the USB interface and network interface are disposed adjacent the other end and exposing in babinet.
9. the full instrument of immune colloid gold according to claim 1, which is characterized in that further include a built-in printing
Machine, the built-in printer connect control interface screen, are used for the result data of print control interface screen display.
10. the full instrument of immune colloid gold according to claim 1, which is characterized in that the light source scanning camera shooting
In device, the angle imaged between light path and LED light source is 45 °, and camera shooting clarity is by turntable driving speed control.
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|---|---|---|---|
| CN201811016700.4A CN108776217B (en) | 2018-09-03 | 2018-09-03 | Immune colloidal gold full quantitative detector |
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| CN111879769A (en) * | 2020-08-05 | 2020-11-03 | 安徽师范大学 | Device for reading medical detection kit |
| CN112230574A (en) * | 2020-07-30 | 2021-01-15 | 深圳加美生物有限公司 | Low-power-consumption multi-channel colloidal gold detection instrument circuit |
| CN112528712A (en) * | 2019-09-19 | 2021-03-19 | 武汉华大智造科技有限公司 | Reagent kit detection device, reagent kit detection method, and storage medium |
| CN112858273A (en) * | 2021-01-13 | 2021-05-28 | 梅里医疗科技(洋浦)有限责任公司 | Detection kit for intelligent hospital, adaptive detection device and detection method |
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Effective date of registration: 20240311 Address after: No. 381 Laodong Road, Diaozhuang Street, Tianning District, Changzhou City, Jiangsu Province, 213000 Patentee after: Changzhou Fubang Biopharmaceutical Co.,Ltd. Country or region after: China Address before: 518000, No. 1004, 10th Floor, Building 10, Shenzhen Biomedical Innovation Industrial Park, No. 14 Jinhui Road, Kengzi Street, Pingshan District, Shenzhen, Guangdong Province Patentee before: SHENZHEN JIAMEI BIOLOGICAL Co.,Ltd. Country or region before: China |