CN108624624A - A method of generating methane using fungi solid state fermentation kitchen garbage - Google Patents
A method of generating methane using fungi solid state fermentation kitchen garbage Download PDFInfo
- Publication number
- CN108624624A CN108624624A CN201810528375.3A CN201810528375A CN108624624A CN 108624624 A CN108624624 A CN 108624624A CN 201810528375 A CN201810528375 A CN 201810528375A CN 108624624 A CN108624624 A CN 108624624A
- Authority
- CN
- China
- Prior art keywords
- kitchen garbage
- solid state
- fungi
- state fermentation
- kitchen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of methods generating methane using fungi solid state fermentation kitchen garbage, include the following steps：Mechanical Crushing after the sorting removal of impurities of a certain amount of kitchen garbage is formed into pending kitchen garbage；5% 20% pending kitchen garbages are chosen, fungi aspergillus awamori is added respectively and aspergillus oryzae carries out the solid matter that solid state fermentation culture is rich in amylase and protease；The cultured solid matter rich in amylase and protease is added to after being hydrolyzed in the mixed water kitchen garbage and forms the hydrolysate rich in small molecule nutriment (glucose and amino acid)；By hydrolysate successively through being separated by solid-liquid separation and producing kitchen garbage hydrolyzate after water-oil separating；Kitchen garbage hydrolyzate is added to anaerobic fermentation tank and produces methane.The present invention, to hydrolyze kitchen garbage, can accelerate the hydrolyzate speed of kitchen garbage by fungi solid state fermentation culture amylase and protease, improve substrate utilization ratio, and then improve methane production.
Description
Technical field
The present invention relates to kitchen garbage processing technology fields more particularly to a kind of utilization fungi solid state fermentation kitchen garbage to produce
The method of raw methane.
Background technology
Kitchen garbage is the chief component of domestic waste, accounts for about 30%-40%, its processing is to solve city
The important link of house refuse problem.Kitchen garbage is based on organic component, and average total starch content reaches 22.3-31.4%, slightly
Protein content reaches 14.2-19.1%, has higher utilization of resources value, is such as filled out using traditional domestic rubbish disposal mode
Bury, burn etc. that all there is certain drawbacks, and Anaerobic Digestion technology is obtained as a kind of more satisfactory processing mode
Extensive use.
However, there is also the science and technical barrier of several keys for current anaerobic fermentation of kitchen waste methane technology：
(1) referring to Fig. 1, the four-stage of anaerobic fermentation of kitchen waste methane, the nutrients in kitchen garbage are shown
Matter (such as starch and protein) usually in the form of macromolecular exists, these macro-nutrients need to generate small molecule through hydrolysis
, just can be by microorganism using fermentation methane after nutriment (such as glucose and amino acid), and this hydrolytic process is meal
The rate-limiting step of kitchen garbage anaerobic fermentation methane, increases the cost of methane phase；
(2) it is different from liquid organic waste, the nutriment majority in kitchen garbage is existing in solid form, is led
It causes substrate utilization ratio low, in turn results in the low output of anaerobic fermentation of kitchen waste methane；
(3) contain a large amount of greases in kitchen garbage, during its anaerobic fermentation methane, these greases can be attached to hair
On ferment methanogen surface, it is suppressed that methanogen activity reduces the specific surface area that substrate is contacted with microorganism,
To influence the methane phase efficiency of anaerobic fermentation methane system.
Therefore in view of the drawbacks of the prior art, it is really necessary to propose a kind of technical solution to solve of the existing technology ask
Topic.
Invention content
In view of this, it is necessory to fast and be easy to the new process of commercial application, the technique profit that provides a kind of hydrolysis rate
Methane is generated with fungi solid state fermentation kitchen garbage, the hydrolyzate speed of kitchen garbage can be accelerated, improves substrate utilization ratio,
Influence of the grease to anaerobic fermentation methane system is reduced, and then improves methane production, reduce methane phase cost.
In order to solve technical problem of the existing technology, technical scheme is as follows：
A method of methane being generated using fungi solid state fermentation kitchen garbage, is included the following steps：
Step S1：Mechanical Crushing after the sorting removal of impurities of a certain amount of kitchen garbage is formed into pending kitchen garbage；
Step S2：Choose 5%-20% in the pending kitchen garbage that step S1 is formed, addition fungi aspergillus awamori and
Aspergillus oryzae carries out the solid matter that solid state fermentation culture is rich in amylase and protease, and by the pending kitchen of rest part
Rubbish adds water to be mixed to form mixed water kitchen garbage；
Wherein, the process of solid state fermentation is to be proportionally added into fungi solution in waiting for fermented garbage from kitchen to carry out solid state fermentation
Amylase and protease are cultivated, the ratio is to wait for that 1L fungi solutions are added in fermented garbage from kitchen per 10kg-20kg；
Step S3：The cultured solid matter rich in amylase and protease in step S2 is added to the mixed water
The hydrolysate rich in small molecule nutriment (glucose and amino acid) is formed after being hydrolyzed in kitchen garbage；
Step S4：The hydrolysate that step S3 is formed successively is produced into kitchen garbage water after separation of solid and liquid and water-oil separating
Solve liquid；
Step S5：Kitchen garbage hydrolyzate is added to anaerobic fermentation tank and produces methane.
In above-mentioned technical proposal, by carrying out solid state fermentation culture amylase and protein to a certain proportion of kitchen garbage
It is hydrolyzed again after enzyme, to greatly accelerate the hydrolyzate speed of kitchen garbage, improves substrate utilization ratio.
As a preferred technical solution, in the step S2, the process of solid state fermentation is that will wait for that fermented garbage from kitchen is put down
Be assigned in 2 culture vessels, according to mass volume ratio 10kg-20kg/L distinguish uniform inoculated fungi aspergillus awamori solution and
On aspergillus oryzae solution to kitchen garbage surface, solid state fermentation 80-100 hour is carried out in insulating box, produce respectively amylase with
Protease.
Amylase and protease are cultivated respectively by different culture vessels, have separated in incubation the mutual of two kinds of enzymes
Effect, improves enzyme training quality and efficiency.
A concentration of the 1 × 10 of the fungi aspergillus awamori solution as a preferred technical solution,5-2.8×108spores/
ml；A concentration of the 1 × 10 of the aspergillus oryzae solution6-6.3×107spores/ml。
During carrying out solid state fermentation in insulating box as a preferred technical solution, constant temperature the temperature inside the box maintains 30
℃。
As a preferred technical solution, in above-mentioned steps S1 by kitchen garbage Mechanical Crushing at the particle of diameter≤5mm.
As a preferred technical solution, by above-mentioned steps S3, hydrolysis temperature control is at 50-55 DEG C, stir speed (S.S.) 300-
500rpm, hydrolyzate time are 20-24h.
Kitchen garbage is hydrolyzed into mix products in 2-6 DEG C of temperature condition in above-mentioned steps S4 as a preferred technical solution,
Separation of solid and liquid, centrifugation rate 8000-10000rpm are realized in lower centrifugation.
As a preferred technical solution, by the mixture after separation of solid and liquid by filter paper vacuum filter carry out water-oil separating with
Grease is removed, the kitchen garbage hydrolyzate rich in glucose and amino acid is obtained.
It in above-mentioned technical proposal, is handled by water-oil separating, reduces influence of the grease to anaerobic fermentation methane system.
The kitchen garbage hydrolyzate produced is added to anaerobic fermentation tank in above-mentioned steps S5 as a preferred technical solution,
Methane is produced in fermentation, wherein control anaerobic fermentation temperature is 35-38 DEG C, rotating speed 150-220rpm.
Compared with prior art, the present invention utilizes culture by fungi solid state fermentation culture amylase and protease
Amylase and proteolytic kitchen garbage, can accelerate the hydrolyzate speed of kitchen garbage, improve substrate utilization ratio；It is logical
Crossing water-oil separating processing reduces influence of the grease to anaerobic fermentation methane system, and then improves methane production, reduces methane phase
Cost.
Description of the drawings
Fig. 1 is anaerobic fermentation of kitchen waste methane process in the prior art.
Fig. 2 is the process flow chart of the present invention.
Following specific embodiment will be further illustrated the present invention in conjunction with above-mentioned attached drawing.
Specific implementation mode
Technical solution provided by the invention is described further below with reference to attached drawing.
Referring to Fig. 2, it show a kind of method generating methane using fungi solid state fermentation kitchen garbage of present invention offer
Flow chart includes the following steps：
Step S1：Mechanical Crushing after the sorting removal of impurities of a certain amount of kitchen garbage is formed into pending kitchen garbage；
Step S2：Choose 5%-20% in the pending kitchen garbage that step S1 is formed, addition fungi aspergillus awamori and
Aspergillus oryzae carries out the solid matter that solid state fermentation culture is rich in amylase and protease, and by the pending kitchen of rest part
Rubbish adds water to be mixed to form mixed water kitchen garbage；
Wherein, the process of solid state fermentation is to be proportionally added into fungi solution in waiting for fermented garbage from kitchen to carry out solid state fermentation
Amylase and protease are cultivated, the ratio is to wait for that 1L fungi solutions are added in fermented garbage from kitchen per 10kg-20kg；
Step S3：The cultured solid matter rich in amylase and protease in step S2 is added to the mixed water
The hydrolysate rich in small molecule nutriment (glucose and amino acid) is formed after being hydrolyzed in kitchen garbage；
Step S4：The hydrolysate that step S3 is formed successively is produced into kitchen garbage water after separation of solid and liquid and water-oil separating
Solve liquid；
Step S5：Kitchen garbage hydrolyzate is added to anaerobic fermentation tank and produces methane.
Wherein, fungi solution is fungi aspergillus awamori (Aspergillus awamori) and aspergillus oryzae
(Aspergillusoryzae)。
In a preferred embodiment, include the following steps：
(1) by Mechanical Crushing after kitchen garbage sorting removal of impurities at the particle of diameter≤5mm；
(2) be respectively added 10g kitchen garbages in 2 culture dishes, respectively uniform inoculated fungi 1ml aspergillus awamoris (2.8 ×
107) or aspergillus oryzae (6.3 × 10 spores/ml6Spores/ml) on kitchen garbage surface, (30 DEG C) progress in insulating box
Solid state fermentation 96h produces amylase and protease；
(3) using the solid matter rich in amylase and protease produced, (dischargeable capacity is in Fermentation
1L), the kitchen garbage of different quality (40-100g) is hydrolyzed, hydrolysis temperature control is at 50-55 DEG C, stir speed (S.S.) 300-
500rpm, hydrolyzate time are 20-24h；
(4) kitchen garbage hydrolysis mix products are centrifuged under the conditions of low temperature (2-6 DEG C), realizes and is separated by solid-liquid separation, centrifugation speed
Rate is 8000-10000rpm；Mixture after centrifugation is gone into oil removing by the graceful No.1 filter paper vacuum filter (water-oil separating) of water
Fat finally obtains the kitchen garbage hydrolyzate rich in glucose and amino acid；
(5) the kitchen garbage hydrolyzate produced is added to anaerobic fermentation tank (dischargeable capacity 1L) fermentation and produces methane, controlled
Anaerobic fermentation temperature processed is 35-38 DEG C, rotating speed 150-220rpm.
Table 1, table 2, the experimental data that table 3 is new_type treatment kitchen garbage through the invention.As Tables 1 and 2 is respectively
The output condition of glucose and amino acid, can be seen that from table 1 and 2 after the kitchen garbage hydrolysis of different quality (40-100g)
The yield of glucose and amino acid increases with the increase of hydrolysis time in kitchen garbage hydrolysate, and is in hydrolysis time
Reach maximum for 24 hours and stablize, realizes the fast hydrolyzing of kitchen garbage；
Table 3 is that the kitchen garbage hydrolysate of different quality (40-100g) adds up methane phase (mL) situation, can from table 3
It to find out, is stepped up as the increase of fermentation time adds up methane phase, and is to reach within the scope of 66-72h in fermentation time
It is maximum.
Glucose yield (g/L) situation after the kitchen garbage hydrolysis of 1 different quality of table (40-100g)
Amino acid production (mg/L) situation after the kitchen garbage hydrolysis of 2 different quality of table (40-100g)
The kitchen garbage hydrolysate of 3 different quality of table (40-100g) adds up methane phase (mL) situation
| Fermentation time (h) | 4% | 6% | 8% | 10% |
| 0 | 0 | 0 | 0 | 0 |
| 2 | 0 | 0 | 0 | 0 |
| 4 | 0 | 0 | 0 | 0 |
| 6 | 40.6 | 54.9 | 102 | 122.8 |
| 18 | 386.3 | 522.4 | 605.6 | 728.9 |
| 24 | 671 | 907.4 | 1007.2 | 1212.1 |
| 28 | 1067.5 | 1443.6 | 1565.2 | 1883.7 |
| 42 | 1403.1 | 1897.3 | 2191.3 | 2637.3 |
| 48 | 1504.8 | 2034.7 | 2422.7 | 2915.7 |
| 52 | 1535.3 | 2076 | 2518 | 3030.4 |
| 66 | 1545.4 | 2089.7 | 2565.6 | 3087.7 |
| 72 | 1565.8 | 2117.2 | 2586.1 | 3112.3 |
The explanation of above example is only intended to facilitate the understanding of the method and its core concept of the invention.It should be pointed out that pair
For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out
Some improvements and modifications, these improvement and modification are also fallen within the protection scope of the claims of the present invention.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest range caused.
Claims (9)
1. a kind of method generating methane using fungi solid state fermentation kitchen garbage, which is characterized in that include the following steps：
Step S1：Mechanical Crushing after the sorting removal of impurities of a certain amount of kitchen garbage is formed into pending kitchen garbage；
Step S2：5%-20% is chosen in the pending kitchen garbage that step S1 is formed, and adds fungi aspergillus awamori and meter Qu
Mould progress solid state fermentation culture is rich in amylase and protease solid matter, and the pending kitchen garbage of rest part is added
Water is mixed to form mixed water kitchen garbage；
Wherein, the process of solid state fermentation is：In waiting for fermented garbage from kitchen, it is proportionally added into fungi solution and carries out solid state fermentation training
Amylase and protease are supported, the ratio is to wait for that 1L fungi solutions are added in fermented garbage from kitchen per 10kg-20kg；
Step S3：The cultured solid matter rich in amylase and protease in step S2 is added to the mixed water kitchen
The hydrolysate rich in small molecule nutriment (glucose and amino acid) is formed after being hydrolyzed in rubbish；
Step S4：The hydrolysate that step S3 is formed successively is hydrolyzed through being separated by solid-liquid separation and producing kitchen garbage after water-oil separating
Liquid；
Step S5：Kitchen garbage hydrolyzate is added to anaerobic fermentation tank and produces methane.
2. the method according to claim 1 for generating methane using fungi solid state fermentation kitchen garbage, which is characterized in that
In the step S2, the process of solid state fermentation is that will wait for that fermented garbage from kitchen is evenly distributed in 2 culture vessels, according to quality
Volume ratio 10kg-20kg/L distinguishes on uniform inoculated fungi aspergillus awamori solution and aspergillus oryzae solution to kitchen garbage surface,
Solid state fermentation is carried out in insulating box 80-100 hours, produce amylase and protease respectively.
3. the method according to claim 2 for generating methane using fungi solid state fermentation kitchen garbage, which is characterized in that institute
State a concentration of the 1 × 10 of fungi aspergillus awamori solution5-2.8×108spores/ml；A concentration of the 1 of the aspergillus oryzae solution ×
106-6.3×107spores/ml。
4. the method according to claim 2 for generating methane using fungi solid state fermentation kitchen garbage, which is characterized in that
During carrying out solid state fermentation in insulating box, constant temperature the temperature inside the box maintains 30 DEG C.
5. the method according to claim 1 or 2 for generating methane using fungi solid state fermentation kitchen garbage, feature exist
In by kitchen garbage Mechanical Crushing at the particle of diameter≤5mm in above-mentioned steps S1.
6. the method according to claim 1 or 2 for generating methane using fungi solid state fermentation kitchen garbage, feature exist
In by above-mentioned steps S3, at 50-55 DEG C, stir speed (S.S.) 300-500rpm, the hydrolyzate time is 20- for hydrolysis temperature control
24h。
7. the method according to claim 1 or 2 for generating methane using fungi solid state fermentation kitchen garbage, feature exist
In, kitchen garbage hydrolysis mix products are centrifuged under 2-6 DEG C of temperature condition in above-mentioned steps S4, realizes and is separated by solid-liquid separation, centrifugation
Rate is 8000-10000rpm.
8. the method according to claim 1 or 2 for generating methane using fungi solid state fermentation kitchen garbage, feature exist
In, by the mixture after separation of solid and liquid by filter paper vacuum filter carry out water-oil separating to remove grease, obtain be rich in glucose
With the kitchen garbage hydrolyzate of amino acid.
9. the method according to claim 1 for generating methane using fungi solid state fermentation kitchen garbage, which is characterized in that on
State in step S5 by the kitchen garbage hydrolyzate produced be added to anaerobic fermentation tank fermentation produce methane, wherein control anaerobism hair
Ferment temperature is 35-38 DEG C, rotating speed 150-220rpm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810528375.3A CN108624624A (en) | 2018-05-26 | 2018-05-26 | A method of generating methane using fungi solid state fermentation kitchen garbage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810528375.3A CN108624624A (en) | 2018-05-26 | 2018-05-26 | A method of generating methane using fungi solid state fermentation kitchen garbage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108624624A true CN108624624A (en) | 2018-10-09 |
Family
ID=63690585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810528375.3A Pending CN108624624A (en) | 2018-05-26 | 2018-05-26 | A method of generating methane using fungi solid state fermentation kitchen garbage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108624624A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336348A (en) * | 2018-11-16 | 2019-02-15 | 江南大学 | A kind of kitchen garbage strengthens aspergillus oryzae and lays eggs white enzyme and the method that promotes excess sludge anaerobism digest production biogas altogether |
| CN110066831A (en) * | 2019-03-13 | 2019-07-30 | 杭州电子科技大学 | The quick natural pond method processed of kitchen garbage |
| CN110305775A (en) * | 2019-07-03 | 2019-10-08 | 河南攸行环保科技有限公司 | A kind of hydrolysis reactor and its application method handling solid waste |
| CN111250046A (en) * | 2020-03-18 | 2020-06-09 | 台州职业技术学院 | Method for preparing biochar by hydrolyzing and carbonizing kitchen waste |
| CN112979355A (en) * | 2021-02-08 | 2021-06-18 | 中新国际联合研究院 | Method for treating kitchen waste by utilizing self-made compound hydrolase |
| CN114350715A (en) * | 2022-01-11 | 2022-04-15 | 武汉轻工大学 | Method for improving anaerobic methane production of kitchen waste |
| CN114904879A (en) * | 2022-03-02 | 2022-08-16 | 上海市园林科学规划研究院 | Preparation method of kitchen waste biogas residue with low phytotoxicity |
| CN115141818A (en) * | 2022-08-23 | 2022-10-04 | 南京农业大学 | Preparation method of biological complex enzyme and application of biological complex enzyme in strengthening kitchen waste fermentation acid production |
| CN115141818B (en) * | 2022-08-23 | 2024-05-28 | 南京农业大学 | Preparation method of biological compound enzyme and application of biological compound enzyme in enhancing fermentation and acid production of kitchen waste |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10235315A (en) * | 1997-02-24 | 1998-09-08 | Mitsubishi Kakoki Kaisha Ltd | Treatment of fluid organic waste |
| CN104561222A (en) * | 2014-03-24 | 2015-04-29 | 浙江大学 | Kitchen waste and sludge hydrogen-producing acidification pretreatment method capable of increasing methane production rate |
| CN106947786A (en) * | 2017-05-12 | 2017-07-14 | 中科院广州能源所盱眙凹土研发中心 | The method that rubbish from cooking prepares biogas |
-
2018
- 2018-05-26 CN CN201810528375.3A patent/CN108624624A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10235315A (en) * | 1997-02-24 | 1998-09-08 | Mitsubishi Kakoki Kaisha Ltd | Treatment of fluid organic waste |
| CN104561222A (en) * | 2014-03-24 | 2015-04-29 | 浙江大学 | Kitchen waste and sludge hydrogen-producing acidification pretreatment method capable of increasing methane production rate |
| WO2015143906A1 (en) * | 2014-03-24 | 2015-10-01 | 浙江大学 | A method for improving the methane production rate through acidification pretreatment of hydrogen production of kitchen waste and sludge |
| CN106947786A (en) * | 2017-05-12 | 2017-07-14 | 中科院广州能源所盱眙凹土研发中心 | The method that rubbish from cooking prepares biogas |
Non-Patent Citations (2)
| Title |
|---|
| WEI HAN等: "Batch dark fermentation from enzymatic hydrolyzed food waste for hydrogen production", 《BIORESOURCE TECHNOLOGY》 * |
| 王永会等: "餐厨垃圾和剩余污泥混合物水解酸化过程研究", 《2013年中国沼气学会学术年会论文集》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336348A (en) * | 2018-11-16 | 2019-02-15 | 江南大学 | A kind of kitchen garbage strengthens aspergillus oryzae and lays eggs white enzyme and the method that promotes excess sludge anaerobism digest production biogas altogether |
| CN110066831A (en) * | 2019-03-13 | 2019-07-30 | 杭州电子科技大学 | The quick natural pond method processed of kitchen garbage |
| CN110305775A (en) * | 2019-07-03 | 2019-10-08 | 河南攸行环保科技有限公司 | A kind of hydrolysis reactor and its application method handling solid waste |
| CN111250046A (en) * | 2020-03-18 | 2020-06-09 | 台州职业技术学院 | Method for preparing biochar by hydrolyzing and carbonizing kitchen waste |
| CN112979355A (en) * | 2021-02-08 | 2021-06-18 | 中新国际联合研究院 | Method for treating kitchen waste by utilizing self-made compound hydrolase |
| CN114350715A (en) * | 2022-01-11 | 2022-04-15 | 武汉轻工大学 | Method for improving anaerobic methane production of kitchen waste |
| CN114904879A (en) * | 2022-03-02 | 2022-08-16 | 上海市园林科学规划研究院 | Preparation method of kitchen waste biogas residue with low phytotoxicity |
| CN115141818A (en) * | 2022-08-23 | 2022-10-04 | 南京农业大学 | Preparation method of biological complex enzyme and application of biological complex enzyme in strengthening kitchen waste fermentation acid production |
| CN115141818B (en) * | 2022-08-23 | 2024-05-28 | 南京农业大学 | Preparation method of biological compound enzyme and application of biological compound enzyme in enhancing fermentation and acid production of kitchen waste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108624624A (en) | A method of generating methane using fungi solid state fermentation kitchen garbage | |
| Wu et al. | Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21 | |
| CN107267427B (en) | Threonine mother liquor recycling method | |
| Oumer et al. | Comparative studies of pectinase production by Bacillus subtilis strain Btk 27 in submerged and solid-state fermentations | |
| Zhu et al. | Bioconversion of yellow wine wastes into microbial protein via mixed yeast-fungus cultures | |
| US11104920B2 (en) | Enzymatic digestion of microalgal biomass for lipid, sugar, and protein recovery | |
| CN104342462A (en) | Dehydroacetic acid (DHA) feed additive prepared from kitchen waste and preparation method thereof | |
| CN112205514A (en) | Method for producing multifunctional spirit vinasse feed | |
| Zohri et al. | Utilization of cheese whey for bio-ethanol production | |
| Bogale | Microbial protein production from agro-industrial wastes as food and feed | |
| CN101824442B (en) | Fermentation production technique of schizogenesis chytrid | |
| CN101613724B (en) | Method for preparing arachidonic acid (ARA) by recycling fungal meal of Mortierella alpine | |
| CN108004015A (en) | A kind of Suaeda salsa seed oil supercritical carbon dioxide extraction process | |
| CN111607622B (en) | Process method for producing 3-hydroxy butanone by using wheat B starch | |
| WO2007083746A1 (en) | Fermentation method for producing ethanol | |
| CN107460223A (en) | A kind of degreasing silkworm chrysalis hydrolysate for microculture and its preparation method and application | |
| RU2127760C1 (en) | Grain-origin ethanol production | |
| JP2008259517A (en) | Equipment and method for producing lactic acid from cellulose | |
| JP4170016B2 (en) | Lactic acid production apparatus and method for producing lactic acid from cellulose | |
| CN114409826B (en) | Method for extracting chitin from periplaneta americana dregs | |
| Vinche et al. | Chitosan: A valuable byproduct of ethanolic fermentation by Rhizopus oryzae | |
| NZ198239A (en) | Two-stage production of ethanol by fermentation | |
| CN106282030A (en) | The Application way of schizochytrium limacinum thalline residue | |
| Balakumar et al. | Palmyrah distillery spent wash for ethanol production by a thermotolerant Saccharomyces cerevisiae S1 at 40° C | |
| JP6737986B2 (en) | Apparatus for producing ethanol from biomass resources and method for producing ethanol from biomass resources |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181009 |
|
| WD01 | Invention patent application deemed withdrawn after publication |