CN108303423A - A kind of simple efficiently fluorescin reagent card digital independent device and method - Google Patents
A kind of simple efficiently fluorescin reagent card digital independent device and method Download PDFInfo
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- CN108303423A CN108303423A CN201810145231.XA CN201810145231A CN108303423A CN 108303423 A CN108303423 A CN 108303423A CN 201810145231 A CN201810145231 A CN 201810145231A CN 108303423 A CN108303423 A CN 108303423A
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 62
- MURGITYSBWUQTI-UHFFFAOYSA-N fluorescin Chemical compound OC(=O)C1=CC=CC=C1C1C2=CC=C(O)C=C2OC2=CC(O)=CC=C21 MURGITYSBWUQTI-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000009795 derivation Methods 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 abstract description 7
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000007547 defect Effects 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000003044 adaptive effect Effects 0.000 description 4
- 238000009792 diffusion process Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 102000006830 Luminescent Proteins Human genes 0.000 description 1
- 108010047357 Luminescent Proteins Proteins 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- Urology & Nephrology (AREA)
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- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Optics & Photonics (AREA)
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Abstract
The invention discloses a kind of simple efficiently fluorescin reagent card digital independent device and method, include Kato equipped with card slot, electric connection MCU and data reading device；The digital independent device is arranged on the mobile route that reagent card is inserted into card slot.Digital independent device reading reagent card data and is transmitted to MCU advantages and is during reagent card is inserted into card slot, the reading of fluorescin data can be carried out by digital independent device during reagent card is inserted directly into Kato, instead of original transmission device, so that the cost of fluorescin detector is lower, occupied space smaller, reading efficiency higher, and without the wear-out defect in machine driving.
Description
Technical field
The present invention relates to a kind of digital independent devices of fluorescin reagent card, also while being related to a kind of fluorescin reagent
The method for reading data of card.
Background technology
Current fluorescin detector generally comprises MCU, Kato, data acquisition device, transmission device and reagent card.
A transmission device driven by stepper motor is needed, after manually reagent card is put into the card slot of Kato, stepping electricity
Machine starts running.Kato uniform motion in metal guide rail is driven by transmission device, data are adopted during uniform motion
Acquisition means start to acquire the data of fluorescin, and send data to MCU.Data sorting after reading is taken out first by MCU
The value of wave crest, secondary peak, to calculate result of laboratory test.
Due to being provided with stepper motor, it is therefore desirable to carry out additional control to stepper motor and program, and need more
Space transmission device.So that fluorescin monitor occurs, complicated, with high costs, collecting efficiency is low, mechanical machine
The technical problems such as structure is easy to wear.
When Kato is at the uniform velocity driven by stepper motor, two uniform parabolas of smooth-going, two parabolical peak values are formed
Ratio is testing result.It, can be directly according to the type of reagent card since data waveform has stability under fixed speed
Number pre-set the data interval for reading peak value.But user is then needed to manually adjust every time using the model of different reagent cards
Corresponding data interval parameter is to adapt to different reagent card models, and otherwise read data can not be proper use of.
Invention content
It is a kind of simple in structure, at low cost present invention aims at providing in order to solve the above-mentioned problems of the prior art
It is honest and clean, collecting efficiency is high, without transmission abrasion fluorescin reagent card digital independent device and method.
A kind of simple efficiently fluorescin reagent card digital independent device of the present invention, includes the card equipped with card slot
Support, which is characterized in that further include the MCU being electrically connected and data reading device；The digital independent device is arranged in reagent
Card insertion enters on the mobile route of card slot, for reading the reagent card data passed through and being transmitted to MCU.
Preferably, the fluorescin reagent card digital independent device further includes first sensor, and described first passes
Sensor setting is in card slot arrival end, for sending the first electric signal to MCU after reagent card is inserted into card slot arrival end.
Preferably, the MCU starts to read the data of digital independent device after receiving the first electric signal.
Preferably, the MCU receives log-on data reading device after the first electric signal and starts to read data.
Preferably, the fluorescin reagent card digital independent device further includes second sensor, and described second passes
Sensor setting is in card slot end, for sending the second electric signal to MCU after reagent card reaches card slot end.
Preferably, the MCU receives the data of stopping reading digital independent device after the second electric signal.
Preferably, the MCU stops reading data and closes digital independent device after receiving the second electric signal.
Preferably, the default record array of the MCU and a derivative array；Digital independent device will be read successively
To data one by one be written record array in；Derivative number is written to record array derivation and by the derivative value of corresponding points successively in MCU
Group；Using search method, using record array index be sequential search record array in first maximum value, if described first most
It is 0 to be worth corresponding derivative value greatly, then records the value that first maximum value is primary peak；Using the method for lookup, to record number
Second maximum value being designated as under group in sequential search record array, if the corresponding derivative value of second maximum value is 0,
Record the value that second maximum value is secondary peak.It needing to illustrate, this MCU specifically filters-and adaptive-peak value carries
The circuit structure and method taken is not the unique data extracting method of above-mentioned fluorescin reagent card digital independent device, above-mentioned glimmering
Photoprotein reagent card digital independent device can complete peak extraction by other disclosed data processing methods in the prior art.
This section of improved MCU is the optimal solution for the irregular fast phenomenon of speed when being inserted into.
A kind of simple efficiently fluorescin reagent card method for reading data of the present invention, which is characterized in that will count
It is arranged on the mobile route that reagent card is inserted into card slot according to reading device；Digital independent device is inserted into the process of card slot in reagent card
Middle reading reagent card data are simultaneously transmitted to MCU.
Preferably, first sensor is set, in card slot end setting second sensor in card slot arrival end；MCU is in reagent
Block log-on data reading device when triggering first sensor and starts to read data, stop when reagent card triggers second sensor
It reads data and closes digital independent device.
One kind of the present invention is simple, and efficiently fluorescin reagent card digital independent device and method, advantage exist
In using characteristics such as more wave crests of sample data, intermittents, the method for the uniform motion of abandoning tradition is directly inserted in reagent card
The reading that can carry out fluorescin data during entering Kato by digital independent device, fills instead of original transmission
It sets.MCU, using filtering, adaptive, peak extraction scheduling algorithm, data is handled after getting data.Make fluorescence egg
The cost of white detector is lower, occupied space smaller, reading efficiency higher, and without the wear-out defect in machine driving.
Description of the drawings
Fig. 1 is the structural schematic diagram of simple efficiently fluorescin reagent card digital independent device of the invention.
Specific implementation mode
As shown in Figure 1, a kind of simple efficiently fluorescin reagent card digital independent device and method of the present invention,
Kato 100 with card slot is arranged in fluorescin detector, and reagents card 200 uses.Reagent card 200 is provided with
Liquid feeding point 210 and the diffusion region 220 being connected with liquid feeding point 210.In the inlet of card slot setting first sensor 130, in card
Second sensor 140 is arranged in slot end.It is described close to the position of sensor setting digital independent device 120 above card slot
Digital independent device 120 is located at reagent card 200 and is inserted on the mobile route of card slot.First sensor 130, second sensor 140
It is electrically connected respectively with MCU110 with data reading device 120.
Operator will detect after drop enters liquid feeding point 210, and testing liquid diffuses in diffusion region 220.It then manually will examination
Agent card 200 is inserted into from the entrance of card slot.First sensor 130 is triggered in card slot arrival end first during insertion, then
Diffusion region 220 moves through digital independent device 120, finally triggers second sensor 140 in card slot end.Digital independent fills
It sets the data that 120 read testing liquid when diffusion region 220 is passed through and is transmitted to MCU110 and carry out subsequent processing.
MCU110 controls the startup or closing of digital independent device 120, and can directly read digital independent device
120 data.Be arranged first sensor 130 effect be when reagent card 200 be inserted into card slot arrival end when, can be right
MCU110 sends out the first electric signal.When receiving the first electric signal, just control digital independent device 120 starts MCU110, can be with
Avoid the long-term standby waste energy of digital independent device 120.In order to avoid MCU110 for a long time to data reading device 120 be in etc.
Pending data reading state just can send out reading instruction when MCU110 receives the first electric signal to data reading device 120, into
One step saves program resource.
The effect that second sensor 140 is arranged is when reagent card 200 is inserted into card slot end, can be right
MCU110 sends out the second electric signal.MCU110 controls the closing of digital independent device 120 when receiving the second electric signal, can save
The standby energy of digital independent device 120.Simultaneously number is waited in order to avoid MCU110 is for a long time in data reading device 120
According to reading state, the instruction of the reading to data reading device 120 can be stopped when MCU110 receives the second electric signal, into one
Step saves program resource.
MCU110 obtains a large amount of acquired data values by digital independent device 120, it is contemplated that the speed being manually inserted into
Uneven, faster or slower, acquired waveform cannot generally form the uniform parabola of smooth-going of two standards.Although this field
Technical staff can obtain two peak values in the waveform, but its by other published data processing methods in the prior art
Method is generally complex, and to this, present invention is alternatively directed to the technical problems to provide a kind of extraction circuit of peak value：The MCU is pre-
If a record array Data [m-1] and a derivative array De [k], wherein parameter m represents record array Data's [m-1]
Length, parameter k represent 0 to the variable between m.Record array is written in the data read by digital independent device one by one successively
In, at the end of plug-in card, the remaining array of array is filled with number 0.The data of array are used (Data [k]-by MCU successively
Data [k-1]) mode of/m differentiates to data, and is written in derivative array De [k]；Using the method for lookup, with record
Array index is first maximum value Data [m1] in sequential search record array, is led if first maximum value is corresponding
Numerical value De [m1]=0 then records the value that first maximum value Data [m1] is primary peak Cmax, is labeled as Cmax=
Data[m1].Using search method, using record array index be sequential search record array in second maximum value Data [m2],
If the corresponding derivative value De [m2]=0 of second maximum value Data [m2], records second maximum value Data
[m2] is the value of secondary peak Tmax, is labeled as Tmax=Data [m2].Finally obtain the value that Tmax divided by Cmax obtains T/C.
There are the improved MCU adaptive various insertion speeds to be formed by curve waveform, no matter first wave or second wave
Waveform how to change because of insertion speed, can accurately obtain corresponding two peaks, while also solving difference
The compatibility issue of the formed different location waveform of reagent card model.Being inserted into different model reagent card every time all need not hand again
The corresponding data interval parameter of adjustment is moved, it is very easy to use quick.
The digital independent device 120 uses high-speed AD (analog-to-digital conversion) equipment, the useful number for operator
According to being wave crest one and wave crest two, the data such as ambient noise belong to invalid data.Know from multi-pass operation result, operator is by reagent
Card 200 is inserted into the overall process of Kato 100 general most fast 200ms or so and completes the action.In 200ms, high-speed A/D device
The up to data of 200K can be acquired, which may insure there are 1000 sampled points in 1ms.It is adaptive by using filtering
Answer, after peak extraction scheduling algorithm calculates, may insure ambient noise completely, the correct differentiation of the second-class peak value of wave crest one, wave crest and
It obtains.
Using characteristics such as more wave crests of sample data, intermittents, the method for the uniform motion of abandoning tradition is manually being inserted into
Reagent card 200 arrive Kato 100 during realize data reading, MCU110 after getting data, using filtering, it is adaptive
It answers, peak extraction scheduling algorithm, data is handled.Compared with prior art, the beneficial effects of the present invention are simplify hardware electricity
Road design, the design of mechanical structure achieve the purpose that more rapidly to obtain result of laboratory test.
For those skilled in the art, technical solution that can be as described above and design are made other each
The corresponding change of kind and deformation, and all these changes and deformation should all belong to the protection model of the claims in the present invention
Within enclosing.
Claims (10)
1. a kind of simple efficiently fluorescin reagent card digital independent device, includes the Kato equipped with card slot, which is characterized in that
Further include the MCU being electrically connected and data reading device；The shifting that card slot is inserted into reagent card is arranged in the digital independent device
On dynamic path, for reading the reagent card data passed through and being transmitted to MCU.
2. simple efficiently fluorescin reagent card digital independent device according to claim 1, which is characterized in that further include
First sensor；The first sensor setting is used for after reagent card is inserted into card slot arrival end in card slot arrival end to MCU
Send the first electric signal.
3. simple efficiently fluorescin reagent card digital independent device according to claim 2, which is characterized in that described
MCU starts to read the data of digital independent device after receiving the first electric signal.
4. simple efficiently fluorescin reagent card digital independent device according to claim 2, which is characterized in that described
MCU receives log-on data reading device after the first electric signal and starts to read data.
5. simple efficiently fluorescin reagent card digital independent device according to claim 1, which is characterized in that further include
Second sensor；The second sensor setting is in card slot end, for being sent to MCU after reagent card reaches card slot end
Second electric signal.
6. simple efficiently fluorescin reagent card digital independent device according to claim 5, which is characterized in that described
MCU receives the data of stopping reading digital independent device after the second electric signal.
7. simple efficiently fluorescin reagent card digital independent device according to claim 5, which is characterized in that described
MCU stops reading data and closes digital independent device after receiving the second electric signal.
8. according to claim 1 to 7, any described simple efficiently fluorescin reagent card digital independent device, feature exist
In the default record array of the MCU and a derivative array；Digital independent device successively by the data read one by one
In write-in record array；Derivative array is written to record array derivation and by the derivative value of corresponding points successively in MCU；Using lookup
Method, to record array index as first maximum value in sequential search record array, if first maximum value is corresponding
Derivative value is 0, then records the value that first maximum value is primary peak；It is suitable to record array index using the method for lookup
Sequence searches second maximum value in record array, if the corresponding derivative value of second maximum value is 0, records described the
Two maximum values are the value of secondary peak.
9. a kind of simple efficiently fluorescin reagent card method for reading data, which is characterized in that digital independent device to be arranged
On the mobile route that reagent card is inserted into card slot；Digital independent device reading reagent card number during reagent card is inserted into card slot
According to and be transmitted to MCU.
10. simple efficiently fluorescin reagent card method for reading data according to claim 9, which is characterized in that in card
Slot arrival end is arranged first sensor, second sensor is arranged in card slot end；MCU is opened when reagent card triggers first sensor
Dynamic digital independent device simultaneously starts to read data, stops reading data and closing data reading when reagent card triggers second sensor
Take device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810145231.XA CN108303423A (en) | 2018-02-12 | 2018-02-12 | A kind of simple efficiently fluorescin reagent card digital independent device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810145231.XA CN108303423A (en) | 2018-02-12 | 2018-02-12 | A kind of simple efficiently fluorescin reagent card digital independent device and method |
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| CN108303423A true CN108303423A (en) | 2018-07-20 |
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ID=62865412
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| CN201810145231.XA Pending CN108303423A (en) | 2018-02-12 | 2018-02-12 | A kind of simple efficiently fluorescin reagent card digital independent device and method |
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