CN108267599A - A kind of portable blood detection and analysis card - Google Patents
A kind of portable blood detection and analysis card Download PDFInfo
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- CN108267599A CN108267599A CN201810304900.3A CN201810304900A CN108267599A CN 108267599 A CN108267599 A CN 108267599A CN 201810304900 A CN201810304900 A CN 201810304900A CN 108267599 A CN108267599 A CN 108267599A
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- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5761—Hepatitis B
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Abstract
The present invention discloses a kind of portable blood detection and analysis card, which is any one in following (I)~(II)：(I) the detection and analysis card is any one in Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and hemoglobin detection reagent card；(II) the detection and analysis card includes at least two in Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and hemoglobin detection reagent card, and adjacent two detection reagent cards are combined and spliced together by connector.Using micro whole blood sample, the detection of blood screening entry is completed on a portable inspectiont analyzing card, accurate interpretation is carried out to testing result with portable blood fluorescence immunity analyzer, and export detection data after progress finishing analysis inside analyzer, tester is made to obtain intuitive detection sentence read result.Portable blood detection and analysis, which block, to be convenient for carrying, and can be detected at any time, and blood screening detects before being suitble to the urgent blood transfusion of field operation, also can operate with all kinds of preoperative, blood safety screening detection before blood sampling.
Description
Technical field
The invention belongs to medical instruments fields, are related to a kind of portable blood detection and analysis card, portable blood detection
Analyzing card cooperation portable blood fluorescence immunity analyzer is for blood safety screening before field operation promptly blood transfusion, preoperative blood sieve
It looks into, the preliminary screening of blood safety before the blood sampling of street corner.
Background technology
The safe screening method of blood mainly has fluoroimmunoassay, solid phase percolation at present, and dry chemical performance rate method is done
Chemical spotting method etc., but be mostly individual event detection in blood screening detection product on the market, detection reagent is mostly sentenced with naked eyes
It reads, using the product of instrument interpretation more or less there is complicated for operation, instrument is heavy, and detection time is long, can not take with oneself
The shortcomings that band.To cover project few there is also detection for a small number of joint-detection products, it is impossible to meet a card and meet multinomial blood sieve
The requirement looked into, and it is low to there is a situation where that detection accuracy is detected compared with individual event.Major blood screening detection product on the market at present
Such as gold mark readout instrument is separately self-existent with dry type chemical analysis instrument, and is carried out for single detection project mostly
Single channel detection, encountering needs entry that can only be carried out when detecting by different detecting instruments, and more people detection when deposit
In serious efficiency.Therefore need to provide a kind of portable blood suitable for entry detection and detection and analysis card,
Portable blood fluorescence immunity analyzer and the safe screening fluoroimmunoassay system of portable blood.
Invention content
Blood screening detection product on the market is mostly individual event detection at present, and there is detection culverts for a small number of joint-detection products
Lid project is not complete, cumbersome, it is impossible to meet the requirement that a card meets multinomial blood screening, be existed using artificial interpretation higher
Error possibility, it is low that multinomial joint-detection has that accuracy of detection is detected compared with individual event.It is an object of the invention to be directed to
Upper technical problem provides a kind of portable blood detection and analysis card.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of portable blood detection and analysis card, the detection and analysis card are any one in following (I)~(II)：
(I) the detection and analysis card for Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and
Any one in hemoglobin detection reagent card；
(II) the detection and analysis card includes Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagent cards
With at least two in hemoglobin detection reagent card, adjacent two detection reagent cards are combined and spliced one by connector
It rises.
As a kind of optimal technical scheme, which includes four Blood grouping reagent card, infectious disease detection examinations
Agent card, ALT detection reagents card and hemoglobin detection reagent card, adjacent two detection reagent cards are combined and spliced by connector
Together.Further preferably, the Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and blood
Lactoferrin detection reagent card splices and combines successively in order.
Still more preferably it is that the Blood grouping reagent card includes three upper cover, pedestal and Blood grouping strip portions
Point, the upper cover and pedestal can closely be fitted into an entirety by plate muscle column, and the Blood grouping strip is arranged on
In the card slot of the pedestal, the upper lid be equipped with location hole and blood group well, the blood group well correspond to described in
The blood group sample application zone of Blood grouping strip；The back side of the pedestal is equipped with positioning plate rib；
Described four detection reagent cards of infectious disease include upper cover, four detection strips of pedestal and infectious disease, three parts,
The upper cover can be closely chimeric into an entirety, described four detection strip difference of infectious disease by plate muscle column with pedestal
It is arranged in the card slot of the pedestal, the upper lid is equipped with observation window, label window, sample application hole and chromatography auxiliary
Liquid well, the observation window correspond to the NC films in four detection strips of the infectious disease, the sample application hole and
Chromatography auxiliary liquid well corresponds to sample application zone and chromatography auxiliary liquid sample application zone in described four detection strips of infectious disease, institute respectively
The back side for stating pedestal is equipped with positioning plate rib；
The ALT detection reagents card includes upper cover, three parts of pedestal and ALT detection strips, the upper cover and bottom
Seat can closely be fitted into an entirety by plate muscle column, and ALT detections strip is arranged in the card slot of the pedestal, institute
The upper lid stated is equipped with ALT detection windows, ALT wells and location hole；The back side of the pedestal is respectively equipped with bottoming hole and determines
Position plate rib；
The hemoglobin detection reagent card includes three upper cover, pedestal and Hb detection strips parts, the upper cover
Can be closely chimeric into an entirety by plate muscle column with pedestal, Hb detections strip is arranged on the card slot of the pedestal
Interior, the upper lid is equipped with hemoglobin well, and the back side of the pedestal is respectively equipped with hemoglobin loophole and positioning
Plate rib.
The connector includes male knot that can be undone by a pull and female knot that can be undone by a pull, passes through public knot that can be undone by a pull between adjacent two individual event detection reagent cards
It is combined and spliced with female knot that can be undone by a pull.
The Blood grouping strip is disposed with substrate, blotting paper III, blotting paper II, blotting paper I, delays from bottom to top
The lap of punching pad and antibody pad, the antibody pad and cushion pad forms blood group sample application zone；
Described four detection strips of infectious disease are disposed with glass pad, antibody pad, NC films and substrate, institute from top to bottom
The one end and one end of the antibody pad for stating glass pad partly overlap, the one end of the other end of the antibody pad and the NC films
Divide overlapping, the other end of the NC films is partially covered with blotting paper；The top that the sample application zone is located at the glass pad is close
The part of antibody pad, described chromatography auxiliary liquid sample application zone are located at the sample application zone down at 3~5mm.
The portable blood detection and analysis card of the present invention employs fluoroimmunoassay, solid phase percolation, dry chemical speed
The detection methods such as rate method, dry chemical spotting method；Portable blood detection and analysis card cooperation portable blood fluorescence immunoassay point
Analyzer is for blood safety screening before field operation promptly blood transfusion, preoperative blood screening, the preliminary screening of blood safety before the blood sampling of street corner.Institute
The portable blood fluorescence immunity analyzer stated is to detect end-point method, detection rates method, the integrated use of Machine Vision Recognition method.
Fluoroimmunoassay, solid phase percolation, dry chemical performance rate method, dry chemical spotting method is at present there is corresponding lists on the market
Item detection reagent.Detect all ripe utilizations on biochemical analyzer of end-point method and detection rates method；Machine Vision Recognition
Method, currently on the market with portable blood fluorescence immunity analyzer of the present invention it is closest have gold mark readout instrument, dry type
Credit analyzer.But fluoroimmunoassay, solid phase percolation, dry chemical performance rate method, dry chemical spotting method is at present on the market
Existing is mostly corresponding individual event detection reagent, and detection reagent is or more using the product of instrument interpretation mostly with naked eyes interpretation
Or it is few there is complicated for operation, instrument is heavy, and detection time is long, the shortcomings that can not carrying.Gold marks reading on the market at present
Instrument is separately self-existent with dry type chemical analysis instrument, and is the single channel inspection carried out for single detection project mostly
It surveys, encountering needs entry that can only be carried out when detecting by different detecting instruments, and there is serious efficiencies.
This patent contains portable blood fluorescence immunity analyzer and blocks with portable blood detection and analysis, specifically for blood
Liquid safe screen is checked to survey and be used：Compared with other detection reagent products, portable blood detection and analysis card covers country will
Ask blood safety screening entry detection, can comprehensive multinomial detection, also can respectively as needed carry out individual event detection, detection
Need sample size few, detection and analysis card only has the general size of credit card, easy to carry；Compared with other detecting instruments, Portable blood
Liquid fluorescence immunity analyzer is small using semi-automatic detection pattern, light-weight, easy to carry, and shock resistance is strong, can be fast
Speed is accurately detected the control of reaction temperature and time, and voluntarily carries out analysis interpretation to testing result, is completed in ten minutes
Testing result exports.
Key Term is explained：
A) it is portable：It is mainly characterized by it is easy to carry, it is light and with stronger shock-resistance features, field situation is suitble to make
With.
B) blood safety screening：Different general individual event screening, the blood safety screening mentioned in this patent are comprehensive
Entry screening, usual blood screening include blood group ABO according to national regulation and are detected with blood group RhD sizings, and alanine amino turns
Move enzyme detection, hemoglobin detection, infectious disease four (hepatitis B surface antibody, AIDS, syphilis, hepatitis) detection；Preoperative blood sieve
It looks into.
C) fluorescence immunoassay：Cross-linking method is immunized with antigen-antibody with fluorescence immunoassay label in this patent.
D) analysis system：It is tested and analyzed in this patent comprising portable blood fluorescence immunity analyzer and portable blood
Card, is collectively referred to as the safe screening fluoroimmunoassay system of portable blood.
Beneficial effects of the present invention：
The portable blood detection and analysis card of the present invention can complete multinomial detection：Using micro whole blood sample, one
It opens and completes the detection of blood screening entry on portable blood detection and analysis card, with portable blood fluorescence immunity analyzer pair
Testing result carries out accurate interpretation, and is exported after detection data is carried out finishing analysis inside analyzer, obtains tester
Intuitive detection sentence read result.Portable blood detection and analysis card is convenient for carrying with portable blood fluorescence immunity analyzer,
It can be detected at any time, blood screening detects before being suitble to the urgent blood transfusion of field operation, also can operate with all kinds of preoperative, blood peace before blood sampling
Full screening detection.
Description of the drawings
Fig. 1：Portable blood detection and analysis card structure figure (multinomial comprehensive detection reagent mode card).
Fig. 2：Portable blood detection and analysis card structure figure (individual event detection reagent mode card).
Fig. 3：Blood grouping reagent card structure diagram.
Fig. 4：Blood grouping strip structure diagram.
Fig. 5：Four detection reagent card structure schematic diagrames of infectious disease.
Fig. 6：Four detection strip structure diagrams of infectious disease.
Fig. 7：ALT detection reagent card structure schematic diagrames.
Fig. 8：Hemoglobin detection reagent card structure schematic diagram.
Fig. 9：Portable blood tests and analyzes card back structures schematic diagram.
Figure 10：The appearance shape figure of portable blood fluorescence immunity analyzer.
Figure 11：The internal structure chart of portable blood fluorescence immunity analyzer.
Figure 12：(A is the back side solid knot of upper shell to the shape and structure figure of portable blood fluorescence immunity analyzer upper shell
Composition, B are the front three-dimensional structure diagram of upper shell).
Figure 13：The structural member stent of portable blood fluorescence immunity analyzer and the shape and structure figure of backlight.
Figure 14：The shape and structure figure of the fixed plate of portable blood fluorescence immunity analyzer.
Figure 15：The shape and structure figure of the bottom plate of portable blood fluorescence immunity analyzer.
Figure 16：(A is detection Kato disk to the shape and structure figure of the detection Kato disk of portable blood fluorescence immunity analyzer
Back side three-dimensional structure diagram, B are the front three-dimensional structure diagram for detecting Kato disk).
Figure 17：(A is that the front of lower housing is three-dimensional to the shape and structure figure of the lower housing of portable blood fluorescence immunity analyzer
Structure chart, B are the back side three-dimensional structure diagram of lower housing).
Specific embodiment
The safe screening immunoassay system of portable blood of the present invention is a kind of inspection for blood safety screening detection
The combination product of card and instrument is surveyed, blood safety screening detection entry detection is completed on a portable detection reagent card,
And pass through portable blood fluorescence immunity analyzer and interpretation, analysis are carried out to testing result, data output obtained in ten minutes
Testing result, it is main to be detected with blood screening before transfusing blood with field operation and the blood safety screening of other situations.It comprises two
A major part：Portable blood detection and analysis card and portable blood fluorescence immunity analyzer.
With reference to specific embodiment to the portable blood detection and analysis card of the present invention and portable blood fluorescence immunoassay
Analyzer is described in detail：
Embodiment 1, portable blood detection and analysis card
Portable blood detection and analysis card employs solid phase percolation, fluorescence immune chromatography method, three kinds of dry chemical detection method
The square foundation science of law.Wherein blood group ABO is with RhD sizing detections using solid phase percolation, and infectious disease four is using fluorescence
Immunochromatographic method, alanine aminotransferase (ALT) and hemoglobin (Hb) are using dry chemical detection method.Portable blood
Test and analyze card structure schematic diagram below figure 1 and Fig. 2：
Portable blood detection and analysis card can use conduct after also decomposable asymmetric choice net as multinomial comprehensive detection reagent mode card
Individual event detection reagent mode card uses, the male and female that each single deck tape-recorder of individual event detection reagent mode card is identified by Fig. 2 (round frame)
Knot that can be undone by a pull is connected, and is combined into the multinomial comprehensive detection reagent mode card of Fig. 1.
Portable blood detection and analysis card can be analyzed to four independent individual event detection reagent cards：Blood grouping reagent card 1,
Four detection reagent cards 2 of infectious disease, 4 four parts of ALT detection reagents card 3 and hemoglobin detection reagent card.Above-mentioned four
Individual event detection reagent card is secondary successively to be combined splicing by male knot that can be undone by a pull 5 and female knot that can be undone by a pull 6, and the bottom of each reagent card is respectively equipped with plate
Rib 7 is used to position.
As shown in figure 3, Blood grouping reagent card 1 divides for 103 3 upper cover 101, pedestal 102 and Blood grouping strip portions
Point, upper cover 101 can be closely chimeric into an entirety by plate muscle column with pedestal 102, and the Blood grouping strip 103 is placed
In the card slot of pedestal 102, the upper cover 101 is equipped with location hole 101-1 and blood group well 101-2, the blood group
Well 101-2 corresponds to the blood group sample application zone of Blood grouping strip 103；There is positioning plate rib 7 on the pedestal 102, be used for
Location reagent is stuck in the placement location on portable blood fluorescence immunity analyzer.
The Blood grouping strip 103 is using diafiltration and chromatography compages, and structure is as shown in figure 4, lowest level base
Plate 103-1 is polyester fiber board, and layer is overlapped III 103-2 of blotting paper, II 103-3 of blotting paper, blotting paper I successively as shown on it
103-4, cushion pad 103-5, antibody pad 103-6.Antibody pads 103-6 and cushion pad 103-5 laps form blood group sample application zone,
It is corresponding with the blood group well 101-2 of Fig. 3.Sample contacts after adding in antibody pad 103-6, adds in auxiliary liquid aid sample later
Be percolated from top to bottom, II 103-3 of blotting paper drains the liquid of diafiltration by chromatography scheme, III 103-2 of blotting paper then into
One step prevents liquid accumulator, reaction is promoted to be normally carried out by surplus liquid diafiltration drainage and toward reversely chromatography.
As shown in figure 5, described four detection reagent cards 2 of infectious disease divide for upper cover 201, pedestal 202 and infectious disease four
203 3 parts of strip are detected, upper cover 201 can be closely chimeric into an entirety, infectious disease four by plate muscle column with pedestal 202
Item detection strip 203 is arranged in the 202 corresponding card slot of pedestal, and upper cover 201 is equipped with observation window 201-1, label window
Mouth 201-2, sample application hole 201-3, chromatography auxiliary liquid well 201-4, observation window 201-1 correspond to four detections of infectious disease
NC film 203-3, sample application hole 201-3 and chromatography auxiliary liquid well 201-4 in strip 203 correspond to infectious disease four respectively
The sample application zone in strip 203 and chromatography auxiliary liquid sample application zone are detected, has positioning plate rib 7 on pedestal 202, for location reagent card
Placement location on portable blood fluorescence immunity analyzer.
The fluorescence immune chromatography method that described four detection strips 203 of infectious disease use, foundation structure is as shown in fig. 6, certainly
Glass pad 203-1, antibody pad 203-2, NC film 203-3 and substrate 203-4 are disposed under above, the glass pads 203-1
One end and one end of antibody pad 203-2 partly overlap, the other end and the NC films 203-3 of antibody pad 203-2
One end partly overlap, the other end of the NC films 203-3 is partially covered with blotting paper 203-5；The sample application zone is located at institute
The top of glass pad 203-1 is stated close to the part of antibody pad 203-2, the chromatography auxiliary liquid sample application zone is located at the sample-adding
Area is down at 4mm.Four detection strips 203 of infectious disease are tied using polyester sheet as substrate 203-4 using the basis of general chromatography
Structure, the albumen of fluorescence signal is marked in coating on antibody pad 203-2, and immune cross-linking reaction, NC are carried out with target detection albumen
Test lines and control lines are coated on film 203-3, as test and quality control index.
As shown in fig. 7, the ALT detection reagents card 3 divides for upper cover 301, pedestal 302 and ALT detection strips 303 3
Part, the upper cover 301 and pedestal 302 can closely be fitted into an entirety by plate muscle column, and ALT detections strip 303 is put
Put in 302 corresponding card slot of pedestal, there is ALT detection window 301-1 in upper cover 301, for portable blood fluorescence immunoassay
Analyzer is used cooperatively, and ALT wells 301-2 is additionally provided in upper cover 301 for detecting the addition of sample, is also set in upper cover 301
There is anchor point 301-3, cooperation detection is stuck in the positioning in portable blood fluorescence immunity analyzer.The back side of pedestal 302 is provided with
Bottoming hole 302-1, cooperation portable blood fluorescence immunity analyzer is to the heat effect of ALT detection strips 303.
As shown in figure 8, the hemoglobin detection reagent card 4 divides for upper cover 401, pedestal 402 and Hb detection strips 403
Three parts, upper cover 401 and pedestal 402 can closely be fitted into an entirety by plate muscle column, and Hb detections strip 403 is placed on
In 402 corresponding card slot of pedestal, there is hemoglobin well 401-1 in upper cover 401, for detecting the addition of sample.Pedestal 402
The back side is provided with hemoglobin loophole 402-1, cooperation portable blood fluorescence immunity analyzer to hemoglobin detection project into
Row digital independent.
The Blood grouping reagent card 1, four detection reagent cards 2 of infectious disease, ALT detection reagents card 3, hemoglobin inspection
4 tetrameric detection reagent card of test agent card can be combined by male knot that can be undone by a pull 5 and female knot that can be undone by a pull 6, be spliced into
Integrated combine detection reagent card.The tetrameric back side is distributed with positioning plate rib 7, for detection reagent to be coordinated to be stuck in just
The placement positioning inside formula Blood fluorescence immunity analysis instrument is taken, portable blood detection and analysis card back structures figure is as shown in Figure 9.
The detection card of the present invention realizes multinomial blood screening safety detection, can independently select individual event mesh, entry group
Close the portable blood detection and analysis card of comprehensive detection；The cooperation portable blood detection point of portable blood fluorescence immunity analyzer
Analysis card uses, and reaction condition is detected for controlling, testing result interpretation processing, Analysis of test results, and testing result output preserves,
Portable blood fluorescence immunity analyzer can coordinate the portable blood detection and analysis card of various combinations to be detected.
2 portable blood fluorescence immunity analyzer of embodiment
Portable blood fluorescence immunity analyzer as a multi-functional analytical instrument, it is main form component for it is existing into
Cooking technique is the integrated function mechanism of a multimode, and the further exploitation for belonging to existing detection technique function is transported with comprehensive
With.
As shown in Figure 10~17, the portable blood fluorescence immunity analyzer of the invention designed, including testing agency, master control
Plate 818, data memory module 810 and the detection for transmitting microscope carrier portable blood detection and analysis card 805 described in embodiment 1
Kato disk 804, the testing agency include four alanine aminotransferase detection module 817, fluorescence infectious disease detection moulds
Block 811, Blood grouping module 812 and hemoglobin detection module 806；
The hemoglobin detection module 806 is fixed on the bottom of the detection Kato disk 804, the detection Kato disk
804 lower section is also equipped with warm bath module 825, and the detection Kato disk 804 is erected on bottom plate 823 and passes through driving device
Its disengaging movement of I driving；The driving device I includes direct current generator 822 and screw rod, and the direct current generator 822 passes through spiral shell
The bar connection detection Kato disk 804, the direct current generator 822 drive the disengaging campaign of the detection Kato disk 804.Institute
The detection Kato disk 804 stated is erected at by two support shafts 824 being set up in parallel on bottom plate 823, and the two of the support shaft 824
End is separately fixed on the bottom plate 823.
Guide rail 816, the alanine aminotransferase detection module are vertically installed on the bottom plate 823
817 are assemblied in by structure stand 813 on the sliding block of the guide rail 816, and the alanine is driven by driving device II
Aminopherase detection module 817 moves up and down in the top of the detection Kato disk 804；The driving device II is step
Stepper motor 815, the stepper motor 815 are fixedly mounted on the vertical connecting plate 820-1 of the fixed plate 820.The instrument
In, the detection Kato disk 804 blocks 805 transmission microscope carrier for portable blood detection and analysis described in embodiment 1.
The Blood grouping module 812 is installed on the module installing zone 819-2 of structural member stent 819, the structure
The fluorescence excitation that backlight 821 is also equipped on the backlight installing zone 819-1 of part stent 819 for four detections of infectious disease shines
It penetrates, the structural member stent 819 is fixedly installed in lower section (the Blood grouping module 812 and the backlight of fixed plate 820
821 between structural member stent 819 and fixed plate 820), described four detection modules 811 of fluorescence infectious disease are fixedly mounted
In the top of the fixed plate 820, described four detection modules 811 of fluorescence infectious disease are fixed in the master control borad 818 stacking
Top；
The fixed plate 820 is fixed on by stud 826 on the bottom plate 823；
Data memory module 810, the warm bath module 825 and each module of the testing agency passes through
The FPC lines connection master control borad 818.
As a kind of optimal technical scheme, shell 802, institute are provided with outside the portable blood fluorescence immunity analyzer
The shell 802 stated divides for upper shell 802-2 and lower housing 802-8.The bottom plate 823 is fixedly mounted on the lower housing
On 802-8, the data memory module 810 is fixed on the panel-mounted position 802-5 of the upper shell 802-2.The analysis
Instrument further includes printer 803, display screen 800, key panel 801, power interface 809, PS2 scanner interfaces 808 and USB interface
807.The printer 803, which is installed on the printer assembling position of the upper shell 802-2 and passes through FPC lines, is connected to interface
Plate 814, the interface board 814 are installed on the lower housing；The display screen 800 is installed on the upper shell 802-
In the display window in 2 centre positions, and pass through FPC lines and be connected to master control borad 818；The key panel 801 is installed on described
The button installation position of upper shell 802-2 simultaneously passes through FPC lines and is connected to master control borad 818.
The power interface 809 inputs for working power and reserve battery charging, the reserve battery are assemblied in
In the battery settling groove of lower housing 802-8.
The printer 803 is preferably thermal printer as data-printing output equipment.800 conduct of display screen
Presentation of information, indicating equipment.The human-computer interaction device that the key panel 801 sets or selects as function.The PS2 scannings
Instrument interface 808 is used as bar code scanner input equipment.The USB interface 807 is as USB principal and subordinate links equipment, it can be achieved that data
Storage, data-transformation facility.The power interface 809 charges for power work power input and reserve battery.The number
It is used to detect the input of the information such as card batch, parameter, and its detection data after test according to the preferably RFID module of memory module 810
Preservation.The guide rail 816 is oriented to for the up and down motion of alanine aminotransferase detection module 817.The stepper motor
815 are used to that alanine aminotransferase detection module 817 to be driven to move up and down.The master control borad 818 is assisted for Comprehensive Control
The normal work of each electric component inside demodulating apparatus.
Each component assembling process of Portable fluorescence immunity analysis instrument of the present invention is as described below：
The built-in printer 803 is fixed by 4 screws, is installed on the printer assembling of 802 upper shell 802-2 of shell
Position 802-3 is connected to interface board 814 by FPC lines.Liquid crystal display 800 is fixed by two screws, is installed on shell
802-1 in the display window in 802 upper shell 802-2 centre positions, master control borad 818 is connected to by FPC lines.Key panel 801
Key panel is affixed on by 3M gluings and assembles position 802-4, master control borad 818 is connected to by FPC lines.Data memory module
(RFID module) 810 is affixed on the memory module panel-mounted position 802-5 of upper shell 802-2 by 3M gluings, is connected by FPC lines
It is connected to master control borad 818.The alanine aminotransferase detection module 817 is assemblied in the cunning of guide rail 816 by structure stand 813
On block, moved up and down by the driving of stepper motor 815.Alanine aminotransferase detection module 817 is connected by FPC leads
Master control borad 818 is connected to as data acquisition and control.
The Blood grouping module 812 is installed on the inspection that blood group is used on the module installing zone 819-2 of structural member stent 819
It surveys and data acquires.Backlight 821 is installed on the backlight installing zone 819-1 of structural member stent 819 upper for infectious disease four
Item fluorescence-exciting radiation.The structural member stent 819 is screwed in 820 lower section of fixed plate.Four inspections of fluorescence infectious disease
Module 811 is surveyed to be screwed above fixed plate 820.Master control borad 818 is fixed on fluorescence infectious disease four by screw stacking
811 top of item detection module.The stepper motor 815 is screwed at fixed plate 820-1.The hemoglobin detection
Module 806 and warm bath module 825 are screwed on pallet 804.The pallet 804 is set up in bottom by support shaft 824
On plate 823, for direct current generator 822 by screw rod connecting trays 804, direct current generator 822 drives pallet disengaging movement.The fixed plate
820 are fixed on by stud 826 on bottom plate 823.The bottom plate 823 is screwed on lower housing 802-8, interface board
814 are screwed on the interface board installation position 802-7 of lower housing.The reserve battery is assemblied in the battery of lower housing
In slot 802-6, fixed by battery cover and screw hole.
Alanine aminotransferase detection module, hemoglobin used in the portable blood fluorescence immunity analyzer
Detection module, Blood grouping module, four detection modules of fluorescence infectious disease and warm bath module those skilled in the art can lead to
Cross conventional means acquisition.
The compact arrangement for the portable blood fluorescence immunity analyzer that the present invention designs has splendid shock resistance effect, letter
Appearance about and key arrangement, also allow operation more succinctly with intuitively.Modules group as structure can be in related market
Upper directly to purchase, assembly material facilitates acquisition.
When portable blood detection and analysis card is used cooperatively with portable blood fluorescence immunity analyzer, portable blood inspection
Survey analyzing card can be both put into portable blood fluorescence immunity analyzer with the whole card of multinomial comprehensive detection reagent mode card and be carried out
Detection, can also be put into portable blood fluorescence immunity analyzer with one project of individual event detection reagent mode card or entry
In be detected, final detection result by portable blood fluorescence immunity analyzer carry out interpretation output.
Portable blood detection and analysis card is divided into four independent detection zones：Blood grouping area, four detection zones of infectious disease,
ALT detection zones, hemoglobin detection zone.Each region can independently be used as individual event detection reagent mode card and portable blood
Fluorescence immunity analyzer is used cooperatively, in this case, reagent card putting on portable blood fluorescence immunity analyzer
Positioning is put mainly to position by the plate rib (square box) of card bottom.
Portable detection reagent card is combined with each other the semi-automatic comprehensive inspection to be formed with portable blood fluorescence immunity analyzer
Examining system is also the main points of the present invention.
The reaction condition of detection is accurately controlled by portable blood fluorescence immunity analyzer, the interpretation of testing result with
The output of data is controlled by portable blood fluorescence immunity analyzer and completed, can be real inside portable blood fluorescence immunity analyzer
Self backup of existing data, and can be uploaded by modes such as data line, bluetooths and backup to corresponding computer, testing result can be with
It needs to make analyzing and processing and export according to client inside detection analysis instrument.
The present invention combines multinomial blood screening detection project, the multinomial blood screening detection of a card is realized, before blood transfusion
The detection method that blood safety screening is provided convenience；Sample size is few needed for detection, improves the convenience of detection, reduces inspection
The volume requirement of test sample sheet reduces detection cost of labor；Portable blood detection and analysis card only has credit card-sized, convenient for taking
Band can do multinomial comprehensive detection, can also separate and do individual event or several combine detections as needed；Detection has been used advanced
Fluorescence immunoassay detection technique and ripe solid phase diafiltration techniques in combination with dry chemical, have accomplished the integrated of multinomial detection technique
It is unified, realize a card integrated use of multinomial Measurement for Biotechnique；Testing result is exported using instrument interpretation and result data,
It the step of reducing the error of artificial interpretation, having used semi-automatic detection operation mode, reduced manual operation, improves
The convenience of operation is detected, detection reagent only has portable size with matched interpretative instrument, handy to carry about, tool
There is stronger shock resistance, Field Operational is suitble to use, can also be used in blood screening situation before other blood transfusions.
Claims (6)
1. a kind of portable inspectiont analyzing card, which is characterized in that the detection and analysis card is any one in following (I)~(II)
Kind：
(I) the detection and analysis card be Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and blood red
Any one in protein assay reagent card；
(II) the detection and analysis card includes Blood grouping reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and blood
At least two in Lactoferrin detection reagent card, adjacent two detection reagent cards are combined and spliced together by connector.
2. portable inspectiont analyzing card according to claim 1, which is characterized in that the detection and analysis card includes Blood grouping
Reagent card, four detection reagent cards of infectious disease, ALT detection reagents card and hemoglobin detection reagent card, adjacent two detections
Reagent card is combined and spliced together by connector.
3. portable inspectiont analyzing card according to claim 2, which is characterized in that the Blood grouping reagent card passes
Catch an illness four detection reagent cards, ALT detection reagents card and hemoglobin detection reagent card splices and combines successively in order.
4. according to the portable inspectiont analyzing card described in claim 1,2 or 3, which is characterized in that
The Blood grouping reagent card includes upper cover, three parts of pedestal and Blood grouping strip, the upper cover and pedestal
An entirety can be closely fitted by plate muscle column, the Blood grouping strip is arranged in the card slot of the pedestal, institute
The upper lid stated is equipped with location hole and blood group well, and the blood group that the blood group well corresponds to the Blood grouping strip adds
Sample area；The back side of the pedestal is equipped with positioning plate rib；
Described four detection reagent cards of infectious disease include upper cover, four detection strips of pedestal and infectious disease, three parts, described
Upper cover and pedestal can be closely chimeric into an entirety by plate muscle column, described four detection strips of infectious disease are set respectively
In the card slot of the pedestal, the upper lid is equipped with observation window, label window, sample application hole and chromatography auxiliary liquid and adds
Sample hole, the observation window correspond to the NC films in described four detection strips of infectious disease, the sample application hole and chromatography
Auxiliary liquid well corresponds to sample application zone and chromatography auxiliary liquid sample application zone in described four detection strips of infectious disease, the bottom respectively
The back side of seat is equipped with positioning plate rib；
The ALT detection reagents card includes upper cover, three parts of pedestal and ALT detection strips, the upper cover and pedestal energy
An entirety is closely enough fitted by plate muscle column, ALT detections strip is arranged in the card slot of the pedestal, described
Upper lid is equipped with ALT detection windows, ALT wells and location hole；The back side of the pedestal is respectively equipped with bottoming hole and positioning plate
Rib；
The hemoglobin detection reagent card includes upper cover, three parts of pedestal and Hb detection strips, the upper cover and bottom
Seat can closely be fitted into an entirety by plate muscle column, and Hb detections strip is arranged in the card slot of the pedestal, institute
The upper lid stated is equipped with hemoglobin well, and the back side of the pedestal is respectively equipped with hemoglobin loophole and positioning plate reinforcement bar
Item.
5. portable inspectiont analyzing card according to claim 1 or 2, which is characterized in that the connector includes public living
Button and female knot that can be undone by a pull, it is combined and spliced by male knot that can be undone by a pull and female knot that can be undone by a pull between adjacent two individual event detection reagent cards.
6. portable inspectiont analyzing card according to claim 4, which is characterized in that the Blood grouping strip from lower and
On be disposed with substrate, blotting paper III, blotting paper II, blotting paper I, cushion pad and antibody pad, the antibody pad and buffering
The lap of pad forms blood group sample application zone；
Described four detection strips of infectious disease are disposed with glass pad, antibody pad, NC films and substrate, the glass from top to bottom
One end and one end of the antibody pad of fibre pad partly overlap, one end part weight of the other end of the antibody pad and the NC films
Folded, the other end of the NC films is partially covered with blotting paper；The sample application zone is located at the top of the glass pad close to antibody
The part of pad, described chromatography auxiliary liquid sample application zone are located at the sample application zone down at 3~5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810304900.3A CN108267599A (en) | 2018-04-08 | 2018-04-08 | A kind of portable blood detection and analysis card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810304900.3A CN108267599A (en) | 2018-04-08 | 2018-04-08 | A kind of portable blood detection and analysis card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108267599A true CN108267599A (en) | 2018-07-10 |
Family
ID=62777580
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810304900.3A Pending CN108267599A (en) | 2018-04-08 | 2018-04-08 | A kind of portable blood detection and analysis card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108267599A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521586A (en) * | 2020-04-23 | 2020-08-11 | 安徽华培生物科技有限公司 | Fluorescence detection reagent card box |
| CN112113955A (en) * | 2020-09-15 | 2020-12-22 | 四川行之智汇知识产权运营有限公司 | Hemoglobin detection card |
| CN112213301A (en) * | 2020-09-15 | 2021-01-12 | 四川行之智汇知识产权运营有限公司 | Collecting and detecting device for hemoglobin in blood |
| CN117825697A (en) * | 2024-03-01 | 2024-04-05 | 中国科学院过程工程研究所 | Hazard factor detection reagent card and detection method |
-
2018
- 2018-04-08 CN CN201810304900.3A patent/CN108267599A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521586A (en) * | 2020-04-23 | 2020-08-11 | 安徽华培生物科技有限公司 | Fluorescence detection reagent card box |
| CN112113955A (en) * | 2020-09-15 | 2020-12-22 | 四川行之智汇知识产权运营有限公司 | Hemoglobin detection card |
| CN112213301A (en) * | 2020-09-15 | 2021-01-12 | 四川行之智汇知识产权运营有限公司 | Collecting and detecting device for hemoglobin in blood |
| CN112213301B (en) * | 2020-09-15 | 2022-03-18 | 四川行之智汇知识产权运营有限公司 | Collecting and detecting device for hemoglobin in blood |
| CN117825697A (en) * | 2024-03-01 | 2024-04-05 | 中国科学院过程工程研究所 | Hazard factor detection reagent card and detection method |
| CN117825697B (en) * | 2024-03-01 | 2024-05-07 | 中国科学院过程工程研究所 | Hazard factor detection reagent card and detection method |
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