CN108102885B - Microbial physiology cultivates test device with integral type - Google Patents
Microbial physiology cultivates test device with integral type Download PDFInfo
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- CN108102885B CN108102885B CN201810162398.7A CN201810162398A CN108102885B CN 108102885 B CN108102885 B CN 108102885B CN 201810162398 A CN201810162398 A CN 201810162398A CN 108102885 B CN108102885 B CN 108102885B
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- 238000012360 testing method Methods 0.000 title claims abstract description 124
- 230000000813 microbial effect Effects 0.000 title claims abstract description 29
- 230000035479 physiological effects, processes and functions Effects 0.000 title claims abstract description 19
- 238000002347 injection Methods 0.000 claims abstract description 81
- 239000007924 injection Substances 0.000 claims abstract description 81
- 244000005700 microbiome Species 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims description 80
- 238000005070 sampling Methods 0.000 claims description 66
- 238000004140 cleaning Methods 0.000 claims description 39
- 239000000243 solution Substances 0.000 claims description 39
- 238000004891 communication Methods 0.000 claims description 17
- 238000005485 electric heating Methods 0.000 claims description 11
- 238000002474 experimental method Methods 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 2
- 238000007599 discharging Methods 0.000 claims 4
- 238000000034 method Methods 0.000 abstract description 24
- 238000000605 extraction Methods 0.000 abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000011550 stock solution Substances 0.000 abstract 1
- 230000000644 propagated effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention discloses an integrated culture test device for microbial physiology, and mainly relates to the field of test devices for microbial physiology. Including electric servo pneumatic cylinder, test platform, stock solution box, it stores chamber, injection chamber, experimental observation sample chamber, middle part box body and places chamber and each check valve to be equipped with the microorganism in the test platform, electric servo pneumatic cylinder, microorganism store chamber, injection chamber, experimental observation sample chamber and put the annular array along test platform central point and distribute. The invention has the beneficial effects that: the error in the whole process before the extraction of the microorganism sample can be reduced, the deviation of the culture condition can be reduced, and the whole process is more time-saving and efficient. Because the microscopic observation is the most important ring for obtaining the test result, the micro observation can enable testers to have more energy to be used for the microscopic observation after the extraction of the microorganism samples, so that the test result is more accurate, and the test efficiency is greatly improved.
Description
Technical Field
The invention relates to the field of test devices for microbial physiology, in particular to an integrated culture test device for microbial physiology.
Background
When a microbial physiology test is carried out, firstly, a microorganism to be tested is cultured and propagated, a culture solution for the test is prepared, then, the microorganism liquid and the culture solution for the test are converged into a test dish for culture test, the suitable test temperature is matched, observation is carried out after a period of time, then, a microorganism sample for the culture test is extracted, and a microorganism slide is prepared for microscopic observation after extraction. In order to ensure the accuracy of data in the test, the steps need to be repeatedly operated, and the average value is obtained through multiple times of test observation. The whole process is not provided with a corresponding integrated culture test device, so that the whole test process is time-consuming and energy-consuming, and when the process is operated every time, each step of a microorganism sample before extraction is unavoidable to have errors and culture conditions to influence the test result. Therefore, an integrated culture test device suitable for microbial physiology is urgently needed at present, so that errors in the whole process before extraction of a microbial sample are reduced, the deviation of culture conditions is reduced, and the whole process is more time-saving and efficient. Because the microscopic observation is the most important ring for obtaining the test result, the micro observation can enable testers to have more energy to be used for the microscopic observation after the extraction of the microorganism samples, so that the test result is more accurate, and the test efficiency is greatly improved.
Disclosure of Invention
The invention aims to provide an integrated culture test device for microbial physiology, which can reduce errors in the whole process before extraction of a microbial sample and reduce deviation of culture conditions, and is more time-saving and efficient in the whole process. Because the microscopic observation is the most important ring for obtaining the test result, the micro observation can enable testers to have more energy to be used for the microscopic observation after the extraction of the microorganism samples, so that the test result is more accurate, and the test efficiency is greatly improved.
In order to achieve the purpose, the invention is realized by the following technical scheme:
an integrated culture test device for microbial physiology comprises an electric servo hydraulic cylinder, a test platform and a liquid storage box, wherein the test platform is a transparent glass platform, a microbial storage cavity, an injection cavity, a test observation sampling cavity and a middle box body placing cavity are arranged in the test platform, the electric servo hydraulic cylinder, the microbial storage cavity, the injection cavity and the test observation sampling cavity are distributed along the central position of the test platform in an annular array manner, the microbial storage cavity is communicated with the injection cavity, a first check valve is arranged at the communication position of the microbial storage cavity and the injection cavity, an injection piston is arranged in the injection cavity, a piston rod is arranged on the injection piston, the electric servo hydraulic cylinder is arranged on the side surface of the test platform, a hydraulic rod of the electric servo hydraulic cylinder is connected with the tail end of the piston rod, an injection head communicated with the test observation sampling cavity is arranged on one side of the injection cavity, which is close to the test observation, the injection head is internally provided with a second one-way valve, the inner wall of the experimental observation sampling cavity is provided with an electric heating plate, the liquid storage box is arranged in the middle box body for placing the cavity, the liquid storage box comprises an experimental culture solution storage cavity and a cleaning cavity which are mutually isolated, the experimental culture solution storage cavity is communicated with the experimental observation sampling cavity, a third one-way valve is arranged at the communication position of the experimental culture solution storage cavity and the experimental observation sampling cavity, the cleaning cavity is communicated with the experimental observation sampling cavity, a fourth one-way valve is arranged at the communication position of the cleaning cavity and the experimental observation sampling cavity, a culture solution injection pipe is arranged at the top of the experimental culture solution storage cavity, a cleaning solution injection pipe is arranged at the top of the cleaning cavity, a liquid discharge pipe is arranged at the bottom of the experimental observation sampling cavity, and a fifth one-way valve is.
The vacuum liquid discharge device is characterized in that a high-pressure air pump and a vacuum collection box are respectively arranged on two sides of the bottom of the liquid discharge pipe, a sixth one-way valve is arranged at the communication position of the high-pressure air pump and the liquid discharge pipe, and a seventh one-way valve is arranged at the communication position of the vacuum collection box and the liquid discharge pipe.
The electric heating plate on the bottom surface of the experimental observation sampling cavity is a conical surface, and a through hole communicated with the top of the liquid discharge pipe is formed in the conical surface.
The top surface of the experimental observation sampling cavity is provided with a sampling hole, and a sealed rubber plug is arranged on the sampling hole.
The bottom of the test platform is provided with a support frame, and the vacuum collection box is placed on the support frame.
The bottom surface of the test platform is provided with a vibrating plate, and the support frame is provided with a vibrating motor.
And a microorganism injection pipe is arranged on the microorganism storage cavity.
Compared with the prior art, the invention has the beneficial effects that:
annular array distributes on the test platform of this device has the electronic servo pneumatic cylinder of multiunit with the specification, the microorganism stores the chamber, the injection chamber, the experimental sample chamber of observing, when using, the whole process that the microorganism sample was drawed can the multiunit go on simultaneously, each liquid quantity in the cultivation process, the incubation time, condition such as cultivation temperature are unanimous basically, the whole in-process error that makes the microorganism sample draw before reduces, reduce the deviation that the cultivation condition exists, save more time in the whole process, the efficiency. Because the microscopic observation is the most important ring for obtaining the test result, the micro observation can enable testers to have more energy to be used for the microscopic observation after the extraction of the microorganism samples, so that the test result is more accurate, and the test efficiency is greatly improved.
Drawings
FIG. 1 is a top view of the present invention.
FIG. 2 is a schematic diagram of the internal structure of the present invention.
Reference numerals shown in the drawings:
1. an electric servo hydraulic cylinder; 2. a test platform; 3. a liquid storage box; 4. a microorganism storage chamber; 5. an injection cavity; 6. the sampling cavity is observed in a test; 7. a middle box body placing cavity; 8. a first check valve; 9. an injection piston; 10. a piston rod; 11. an injection head; 12. a second one-way valve; 13. an electrical heating plate; 14. a culture solution storage cavity for test; 15. a cleaning chamber; 16. a third check valve; 17. a fourth check valve; 18. a culture solution injection tube; 19. a cleaning solution injection tube; 20. a liquid discharge pipe; 21. a fifth check valve; 22. a high pressure air pump; 23. a vacuum collection box; 24. a sixth check valve; 25. a seventh check valve; 26. a sampling hole; 27. sealing the rubber plug; 28. a support frame; 29. a vibrating plate; 30. a vibration motor; 31. microorganism injection tube.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.
The invention relates to an integrated culture test device for microbial physiology, which comprises an electric servo hydraulic cylinder 1, a test platform 2 and a liquid storage box 3, wherein the test platform 2 is a transparent glass platform, and the test platform 2 is set to be the transparent glass platform, so that observation is more convenient. Be equipped with microorganism in test platform 2 and store chamber 4, injection chamber 5, experimental observation sample chamber 6, middle part box body and place chamber 7, electric servo hydraulic cylinder 1, microorganism store chamber 4, injection chamber 5, experimental observation sample chamber 6 and put the annular array along test platform 2 central point and distribute, so, the whole process that the microorganism sample was drawed can the multiunit go on simultaneously. The microorganism storage cavity 4 is communicated with the injection cavity 5, a first one-way valve 8 is arranged at the communication position of the microorganism storage cavity 4 and the injection cavity 5, an injection piston 9 is arranged in the injection cavity 5, a piston rod 10 is arranged on the injection piston 9, the electric servo hydraulic cylinder 1 is arranged on the side surface of the test platform 2, a hydraulic rod of the electric servo hydraulic cylinder 1 is connected with the tail end of the piston rod 10, an injection head 11 communicated with the test observation sampling cavity 6 is arranged on one side of the injection cavity 5 close to the test observation sampling cavity 6, a second one-way valve 12 is arranged in the injection head 11, when the device is used, the hydraulic rod of the electric servo hydraulic cylinder 1 drives the piston rod 10 to move towards one end far away from the injection head 11, the second one-way valve 12 seals a pipeline of the injection head 11 in the process, the first one-way valve 8 is opened, and microorganism liquid in the microorganism storage cavity 4 enters the injection cavity 5 through the first, after the injection cavity 5 is filled with the microbial liquid, the hydraulic rod of the electric servo hydraulic cylinder 1 drives the piston rod 10 to move towards one end close to the injection head 11, the second one-way valve 12 opens the pipeline of the injection head 11 in the process, the second one-way valve 12 is opened, and the microbial liquid in the injection cavity 5 enters the experimental observation sampling cavity 6 through the first one-way valve 8 under the action of the piston rod 10 and the injection piston 9. The inner wall of the experimental observation sampling cavity 6 is provided with an electric heating plate 13, the liquid storage box 3 is arranged in the middle box body placing cavity 7, the liquid storage box 3 comprises an experimental culture solution storage cavity 14 and a cleaning cavity 15 which are isolated from each other, the experimental culture solution storage cavity 14 is communicated with the experimental observation sampling cavity 6, a third one-way valve 16 is arranged at the communication position of the experimental culture solution storage cavity 14 and the experimental observation sampling cavity 6, the experimental culture solution is injected into the experimental culture solution storage cavity 14 through an injector, then the experimental culture solution in the experimental culture solution storage cavity 14 is injected into the experimental observation sampling cavity 6 again, in the process, the third one-way valve 16 is opened, the experimental culture solution injected into the experimental observation sampling cavity 6 is mixed with the microbial liquid injected before to carry out culture experiment, the electric heating plate 13 provides proper culture experiment temperature, culture experiments were performed after each interval. The cleaning cavity 15 is communicated with the experimental observation sampling cavity 6, a fourth one-way valve 17 is arranged at the communication position of the cleaning cavity 15 and the experimental observation sampling cavity 6, a culture solution injection tube 18 is arranged at the top of the experimental culture solution storage cavity 14, and the experimental culture solution is injected into the experimental culture solution storage cavity 14 through the culture solution injection tube 18 by the injector. The top of the cleaning cavity 15 is provided with a cleaning liquid injection tube 19, after the test, the cleaning liquid is injected into the cleaning cavity 15 through the cleaning liquid injection tube 19, then the cleaning liquid in the cleaning cavity 15 is injected into the test observation sampling cavity 6, and the fourth one-way valve 17 is opened in the process, so that the test observation sampling cavity 6 is cleaned. A liquid discharge pipe 20 is arranged at the bottom of the test observation sampling cavity 6, a fifth one-way valve 21 is arranged in the liquid discharge pipe 20, after cleaning, the fifth one-way valve 21 is opened, and cleaning liquid after cleaning is discharged through the liquid discharge pipe 20.
The two sides of the bottom of the liquid discharge pipe 20 are respectively provided with a high-pressure air pump 22 and a vacuum collection box 23, a sixth one-way valve 24 is arranged at the communication position of the high-pressure air pump 22 and the liquid discharge pipe 20, and a seventh one-way valve 25 is arranged at the communication position of the vacuum collection box 23 and the liquid discharge pipe 20. Before the cleaned cleaning liquid is discharged, the high-pressure air pump 22 flushes the sixth one-way valve 24 to act on the fifth one-way valve 21 in the liquid discharge pipe 20, liquid leakage of the fifth one-way valve 21 is avoided, when the cleaned cleaning liquid needs to be discharged, the high-pressure air pump 22 does not work any more, the seventh one-way valve 25 is opened, the sixth one-way valve 24 is naturally closed, the fifth one-way valve 21 in the liquid discharge pipe 20 is opened under the action of air pressure difference, and the cleaned cleaning liquid is discharged to sequentially pass through the fifth one-way valve 21 and the seventh one-way valve 25 and then is discharged into the vacuum collection box 23.
The electric heating plate 13 on the bottom surface of the experimental observation sampling cavity 6 is a conical surface, and a through hole communicated with the top of the liquid discharge pipe 20 is formed in the conical surface. By providing the bottom surface of the electric heating plate 13 with a tapered surface, the microbial test culture liquid remaining after sampling and the cleaning liquid after cleaning can be easily discharged from the test observation sampling chamber 6 into the vacuum collection box 23.
The top surface of the experimental observation sampling cavity 6 is provided with a sampling hole 26, and a sealing rubber plug 27 is arranged on the sampling hole 26. When the microorganism test culture liquid in the test observation sampling cavity 6 is sampled, the closed rubber plug 27 is taken down and is inserted into the test observation sampling cavity 6 by a syringe for sampling.
The bottom of the test platform 2 is provided with a support frame 28, and the vacuum collection box 23 is placed on the support frame 28. The support frame 28 is used for supporting the vacuum collection box 23.
The bottom surface of the test platform 2 is provided with a vibrating plate 29, and the support frame 28 is provided with a vibrating motor 30. The vibration motor 30 can drive the vibration plate 29 to vibrate, and can vibrate the microorganism test culture liquid in the test observation sampling cavity 6, so that the microorganism test culture liquid can be mixed uniformly more quickly.
And a microorganism injection pipe 31 is arranged on the microorganism storage cavity 4. The microorganism liquid is injected into the microorganism storage chamber 4 through the microorganism injection tube 31.
The using method comprises the following steps:
when the device is used, microorganisms to be tested are cultured and propagated in each microorganism storage cavity 4, and culture solution for testing is prepared and then injected into the culture solution storage cavity 14 for testing. After a period of time, the electric servo hydraulic cylinder 1 is started, the hydraulic rod of the electric servo hydraulic cylinder 1 drives the piston rod 10 to move towards one end far away from the injection head 11, the second one-way valve 12 seals the pipeline of the injection head 11 in the process, the first one-way valve 8 is opened, the microorganism liquid in the microorganism storage cavity 4 enters the injection cavity 5 through the first one-way valve 8, after the injection cavity 5 is filled with the microorganism liquid, the hydraulic rod of the electric servo hydraulic cylinder 1 drives the piston rod 10 to move towards one end close to the injection head 11, the second one-way valve 12 opens the pipeline of the injection head 11 in the process, the second one-way valve 12 is opened, the microorganism liquid in the injection cavity 5 enters the experimental observation sampling cavity 6 through the first one-way valve 8 under the action of the piston rod 10 and the injection piston 9. At the same time, the culture solution for test in the culture solution storage chamber 14 for test is again injected into the test observation sampling chamber 6, and in this process, the third check valve 16 is opened, and the culture solution for test injected into the test observation sampling chamber 6 is mixed with the previously injected microorganism liquid to perform the culture test, and the electric heating plate 13 supplies a suitable culture test temperature to perform the culture test observation after a certain period of time. The vibration motor 30 can drive the vibration plate 29 to vibrate, and can vibrate the microorganism test culture liquid in the test observation sampling cavity 6, so that the microorganism test culture liquid can be mixed uniformly more quickly.
Then, the microorganism sample of the culture test in the test observation sampling cavity 6 is extracted at a proper time, and a microorganism slide is manufactured after the extraction for microscopic observation.
After the test, the cleaning liquid is injected into the cleaning cavity 15 through the cleaning liquid injection tube 19, then the cleaning liquid in the cleaning cavity 15 is injected into the test observation sampling cavity 6, and the fourth one-way valve 17 is opened in the process to clean the test observation sampling cavity 6. A liquid discharge pipe 20 is arranged at the bottom of the test observation sampling cavity 6, a fifth one-way valve 21 is arranged in the liquid discharge pipe 20, after cleaning, the fifth one-way valve 21 is opened, and cleaning liquid after cleaning is discharged through the liquid discharge pipe 20.
Before the cleaned cleaning liquid is discharged, the high-pressure air pump 22 flushes the sixth one-way valve 24 to act on the fifth one-way valve 21 in the liquid discharge pipe 20, liquid leakage of the fifth one-way valve 21 is avoided, when the cleaned cleaning liquid needs to be discharged, the high-pressure air pump 22 does not work any more, the seventh one-way valve 25 is opened, the sixth one-way valve 24 is naturally closed, the fifth one-way valve 21 in the liquid discharge pipe 20 is opened under the action of air pressure difference, and the cleaned cleaning liquid is discharged to sequentially pass through the fifth one-way valve 21 and the seventh one-way valve 25 and then is discharged into the vacuum collection box 23.
Example (b):
the invention relates to an integrated culture test device for microbial physiology, which comprises an electric servo hydraulic cylinder 1, a test platform 2 and a liquid storage box 3, wherein the test platform 2 is a transparent glass platform, and the test platform 2 is set to be the transparent glass platform, so that observation is more convenient. Be equipped with microorganism in test platform 2 and store chamber 4, injection chamber 5, experimental observation sample chamber 6, middle part box body and place chamber 7, electric servo hydraulic cylinder 1, microorganism store chamber 4, injection chamber 5, experimental observation sample chamber 6 and put the annular array along test platform 2 central point and distribute, so, the whole process that the microorganism sample was drawed can the multiunit go on simultaneously. The microorganism storage cavity 4 is communicated with the injection cavity 5, a first one-way valve 8 is arranged at the communication position of the microorganism storage cavity 4 and the injection cavity 5, an injection piston 9 is arranged in the injection cavity 5, a piston rod 10 is arranged on the injection piston 9, the electric servo hydraulic cylinder 1 is arranged on the side surface of the test platform 2, a hydraulic rod of the electric servo hydraulic cylinder 1 is connected with the tail end of the piston rod 10, an injection head 11 communicated with the test observation sampling cavity 6 is arranged on one side of the injection cavity 5 close to the test observation sampling cavity 6, a second one-way valve 12 is arranged in the injection head 11, an electric heating plate 13 is arranged on the inner wall of the test observation sampling cavity 6, the liquid storage box 3 is arranged in the middle box body placing cavity 7, the liquid storage box 3 comprises a test culture solution storage cavity 14 and a cleaning cavity 15 which are mutually isolated, the test culture solution storage cavity 14 is communicated with the test observation sampling cavity 6, and experimental culture solution storage chamber 14 and experimental observation sample chamber 6 intercommunication department are equipped with third check valve 16, wash chamber 15 and experimental observation sample chamber 6 and be linked together, and wash chamber 15 and experimental observation sample chamber 6 intercommunication department and be equipped with fourth check valve 17, experimental culture solution storage chamber 14 top is equipped with culture solution injection pipe 18, wash chamber 15 top and be equipped with washing liquid injection pipe 19, experimental observation sample chamber 6 bottom is equipped with fluid-discharge tube 20, be equipped with fifth check valve 21 in the fluid-discharge tube 20. The third check valve 16, the fourth check valve 17 and the fifth check valve 21 are all electromagnetic control valves. The two sides of the bottom of the liquid discharge pipe 20 are respectively provided with a high-pressure air pump 22 and a vacuum collection box 23, a sixth one-way valve 24 is arranged at the communication position of the high-pressure air pump 22 and the liquid discharge pipe 20, and a seventh one-way valve 25 is arranged at the communication position of the vacuum collection box 23 and the liquid discharge pipe 20. The sixth check valve 24 and the seventh check valve 25 are electromagnetic control valves. The electric heating plate 13 on the bottom surface of the experimental observation sampling cavity 6 is a conical surface, and a through hole communicated with the top of the liquid discharge pipe 20 is formed in the conical surface. The top surface of the experimental observation sampling cavity 6 is provided with a sampling hole 26, and a sealing rubber plug 27 is arranged on the sampling hole 26. The bottom of the test platform 2 is provided with a support frame 28, the vacuum collection box 23 is placed on the support frame 28, the bottom surface of the test platform 2 is provided with a vibration plate 29, and the support frame 28 is provided with a vibration motor 30. And a microorganism injection pipe 31 is arranged on the microorganism storage cavity 4.
Claims (7)
1. The utility model provides a microbial physiology cultivates test device with integral type which characterized in that: comprises an electric servo hydraulic cylinder (1), a test platform (2) and a liquid storage box (3), wherein the test platform (2) is a transparent glass platform, the test platform (2) is internally provided with a microorganism storage cavity (4), an injection cavity (5), a test observation sampling cavity (6) and a middle box body placing cavity (7), the electric servo hydraulic cylinder (1), the microorganism storage cavity (4), the injection cavity (5) and the test observation sampling cavity (6) are distributed along the central position of the test platform (2) in an annular array manner, the microorganism storage cavity (4) is communicated with the injection cavity (5), a first check valve (8) is arranged at the communication position of the microorganism storage cavity (4) and the injection cavity (5), an injection piston (9) is arranged in the injection cavity (5), a piston rod (10) is arranged on the injection piston (9), the electric servo hydraulic cylinder (1) is arranged on the side surface of the test platform (2), and the hydraulic rod of the electric servo hydraulic cylinder (1) is connected with the tail end of the piston rod (10), one side of the injection cavity (5) close to the experimental observation sampling cavity (6) is provided with an injection head (11) communicated with the experimental observation sampling cavity (6), a second one-way valve (12) is arranged in the injection head (11), the inner wall of the experimental observation sampling cavity (6) is provided with an electric heating plate (13), the liquid storage box (3) is arranged in the middle box body placing cavity (7), the liquid storage box (3) comprises a culture solution storage cavity (14) for experiment and a cleaning cavity (15) which are mutually isolated, the culture solution storage cavity (14) for experiment is communicated with the experimental observation sampling cavity (6), a third one-way valve (16) is arranged at the communication position of the culture solution storage cavity (14) for experiment and the experimental observation sampling cavity (6), and the cleaning cavity (15) is communicated with the experimental observation sampling cavity (6), and wash chamber (15) and experimental observation sample chamber (6) intercommunication department and be equipped with fourth check valve (17), experimental culture solution is stored chamber (14) top and is equipped with culture solution injection pipe (18) with the experiment, it is equipped with washing liquid injection pipe (19) to wash chamber (15) top, experimental observation sample chamber (6) bottom is equipped with fluid-discharge tube (20), be equipped with fifth check valve (21) in fluid-discharge tube (20).
2. The integrated culture test device for microbial physiology according to claim 1, characterized in that: the liquid discharging device is characterized in that a high-pressure air pump (22) and a vacuum collecting box (23) are respectively arranged on two sides of the bottom of the liquid discharging pipe (20), a sixth one-way valve (24) is arranged at the communication position of the high-pressure air pump (22) and the liquid discharging pipe (20), and a seventh one-way valve (25) is arranged at the communication position of the vacuum collecting box (23) and the liquid discharging pipe (20).
3. The integrated culture test device for microbial physiology according to claim 1, characterized in that: the electric heating plate (13) on the bottom surface of the experimental observation sampling cavity (6) is a conical surface, and a through hole communicated with the top of the liquid discharge pipe (20) is formed in the conical surface.
4. The integrated culture test device for microbial physiology according to claim 1, characterized in that: the top surface of the experimental observation sampling cavity (6) is provided with a sampling hole (26), and a sealing rubber plug (27) is arranged on the sampling hole (26).
5. The integrated culture test device for microbial physiology according to claim 2, characterized in that: the bottom of the test platform (2) is provided with a support frame (28), and the vacuum collection box (23) is placed on the support frame (28).
6. The integrated culture test device for microbial physiology according to claim 5, wherein: the bottom surface of the test platform (2) is provided with a vibrating plate (29), and the support frame (28) is provided with a vibrating motor (30).
7. The integrated culture test device for microbial physiology according to claim 1, characterized in that: and a microorganism injection pipe (31) is arranged on the microorganism storage cavity (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810162398.7A CN108102885B (en) | 2018-02-27 | 2018-02-27 | Microbial physiology cultivates test device with integral type |
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| CN201810162398.7A CN108102885B (en) | 2018-02-27 | 2018-02-27 | Microbial physiology cultivates test device with integral type |
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| CN108102885B true CN108102885B (en) | 2021-04-20 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107060709A (en) * | 2017-03-14 | 2017-08-18 | 海安县石油科研仪器有限公司 | Microbial oil displacement acts on three-dimensional physical simulation experimental provision |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107060709A (en) * | 2017-03-14 | 2017-08-18 | 海安县石油科研仪器有限公司 | Microbial oil displacement acts on three-dimensional physical simulation experimental provision |
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