CN107436299B - Method and device for automatically analyzing and adjusting brightness of biological fluorescence imaging - Google Patents
Method and device for automatically analyzing and adjusting brightness of biological fluorescence imaging Download PDFInfo
- Publication number
- CN107436299B CN107436299B CN201710610519.5A CN201710610519A CN107436299B CN 107436299 B CN107436299 B CN 107436299B CN 201710610519 A CN201710610519 A CN 201710610519A CN 107436299 B CN107436299 B CN 107436299B
- Authority
- CN
- China
- Prior art keywords
- brightness
- excitation light
- light intensity
- fluorescence imaging
- intensity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/02—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the intensity of light
- G02B26/023—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the intensity of light comprising movable attenuating elements, e.g. neutral density filters
Abstract
The invention relates to a method and a device for automatically analyzing and adjusting the brightness of biological fluorescence imaging, which comprises the following steps of S1: acquiring a fluorescence imaging brightness value and an excitation light intensity of a current sample, and calculating a brightness difference value according to a preset brightness reference value; s2: adjusting the intensity of the excitation light in a grading manner to adjust the fluorescence imaging brightness value of the current sample to a brightness reference value; s3: fitting a relation formula of the fluorescence imaging brightness value and the excitation light intensity: i isF∞Fm(ii) a S4: according to the method, the relation formula between the fluorescence imaging light intensity and the excitation light intensity is automatically calculated through the preset brightness reference value, and the light source is automatically adjusted to enable the fluorescence intensity to reach the brightness reference value, so that the analysis of the relation between the fluorescence light intensity and the excitation light intensity is simplified, the manual operation is reduced, the acquisition efficiency is improved, and the image quality of fluorescence imaging is ensured.
Description
Technical Field
The invention relates to a method and a device for automatically analyzing and adjusting the brightness of biological fluorescence imaging, belonging to the field of biological fluorescence imaging.
Background
Biological Optical Imaging (Optical Imaging) refers to a method for obtaining biological information from cells or tissues or even organisms by using Optical detection means in combination with Optical detection molecules to image the cells or tissues. If bio-optical imaging is limited to the visible and near infrared light ranges, different bio-optical imaging depending on the detection mode can be classified into fluorescence imaging, bioluminescence imaging, photoacoustic imaging, optical tomographic imaging, and the like.
The bio-optical imaging is widely applied due to the advantages of mature development of detection instruments, high sensitivity, high contrast, high resolution, direct imaging, high imaging speed, nondestructive detection and the like. It has important practical significance and application prospect in the aspects of searching pathogenesis, clinical manifestation and genetic lesion of diseases, understanding corresponding physiological and pathological information, disease diagnosis, development of new medical means and the like.
In the field of biological fluorescence imaging, the relationship between the intensity of excitation light irradiated by a biological sample and the intensity of the excited fluorescence sometimes needs to be analyzed, the current method adopting manual adjustment and calculation is time-consuming and difficult to ensure precision, the intensity of laser excitation light sometimes needs to be adjusted to enable the biological sample to obtain a clear fluorescence image, and the situation that the image is not clear due to over-dark or over-bright of the fluorescence image is avoided.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: in order to overcome the problems, a method and a device for automatically analyzing and adjusting the brightness of the bioluminescence imaging, which can be automatically adjusted, are provided.
The technical scheme adopted by the invention for solving the technical problems is as follows: a method for automatically analyzing and adjusting the brightness of biological fluorescent imaging comprises the following steps
S1: acquiring a fluorescence imaging brightness value and an excitation light intensity of a current sample, and calculating a brightness difference value according to the fluorescence imaging brightness value and a preset brightness reference value;
s2: adjusting the intensity of the excitation light in a grading manner according to the brightness difference value, adjusting the fluorescence imaging brightness value of the current sample to a brightness reference value, and recording the intensity of the excitation light and the corresponding fluorescence imaging brightness value each time;
s3: fitting a relation formula between the fluorescence imaging brightness value and the excitation light intensity according to the recorded excitation light intensity and the fluorescence imaging brightness value: i isF∝Fm(F is excitation light intensity, I)FIs the fluorescence imaging brightness value), and the value of m is obtained according to the formula;
s4: and calculating the excitation light intensity corresponding to the sample fluorescence imaging as the brightness reference value according to the formula, and adjusting the current excitation light intensity to the excitation light intensity corresponding to the brightness reference value.
Preferably, the "adjusting the intensity of the excitation light in fractions" is specifically: the intensity of the excitation light is adjusted by rotating the continuously variable metal film filter to different angles for a plurality of times.
Preferably, the continuously variable metal film filter is controlled by the stepping motor to rotate at different angles, the continuously variable metal film filter is continuously rotated by the steps of the stepping motor during rotation, different attenuation levels are generated for the light source by driving the continuously variable metal film filter to rotate at different angles, and the different steps of the rotation of the stepping motor and the excitation light intensity corresponding to the steps are calibrated and recorded into the light flux level table.
Preferably, in the calibration recording into the light flux level table, by the formula:
excitation light intensity F is 10-ODAnd I (OD is optical density of the optical filter, and I is light source intensity), a luminous flux level table of which the step number of each stepping motor corresponds to the excitation light intensity is worked out, the step number of the corresponding stepping motor can be found through the excitation light intensity at the moment, and the luminous flux level table can be found through the current step number of the stepping motor to obtain the excitation light intensity value.
The device for automatically analyzing and adjusting the brightness of the biological fluorescence imaging comprises a continuous variable metal film filter driven to rotate by a driving device, wherein a sample and a light source are correspondingly arranged on two sides of the continuous variable metal film filter respectively, a collecting camera is further arranged on the rear side of the sample and is connected with a controller, and the controller is further connected with a display device and the driving device.
Preferably, the driving device is a stepping motor, and the stepping motor drives the continuous variable metal film optical filter to rotate through a rotating shaft.
Preferably, the controller can be a computer, an ARM, an FPGA or a DSP embedded platform.
The invention has the beneficial effects that: according to the invention, through the preset brightness reference value, the relation formula between the fluorescence imaging brightness value and the excitation light intensity is automatically calculated, and the light source is automatically adjusted to enable the fluorescence imaging brightness value to reach the brightness reference value, so that the analysis of the relation between the fluorescence imaging brightness value and the excitation light intensity is simplified, the manual operation is reduced, the acquisition efficiency is greatly improved, and the image quality of fluorescence imaging is ensured.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a flow chart of a method for automated analysis and adjustment of bioluminescence imaging brightness in accordance with the present invention;
FIG. 2 is a structural diagram of an embodiment of the automatic analyzing and adjusting device for biological fluorescence imaging brightness according to the present invention.
The labels in the figure are: 1-collection camera, 2-display device, 3-sample, 4-continuous variable metal film optical filter, 5-rotating shaft, 6-driving and rotating device, 7-light source, 8-controller, 9-exciting light and 10-fluorescence.
Detailed Description
The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic views illustrating only the basic structure of the present invention in a schematic manner, and thus show only the constitution related to the present invention.
Example 1
The method for automatically analyzing and adjusting the brightness of the bioluminescence imaging provided by the invention as shown in figure 1 comprises the following steps
S1: the method comprises the steps of obtaining the current fluorescence imaging brightness value and the excitation light intensity of a sample 3, and calculating a brightness difference value according to the fluorescence imaging brightness value and a preset brightness reference value, wherein the preset brightness reference value is the average value of all pixels of an image when a sample fluorescence image is clear, and the reference value can be obtained by conventional limited calculation of a person skilled in the art.
S2: adjusting the intensity of the excitation light in a grading manner according to the brightness difference value, so that the current fluorescence imaging brightness value of the sample 3 is adjusted to a brightness reference value, and recording the intensity of the excitation light and the corresponding fluorescence imaging brightness value each time;
s3: fitting a relation formula between the fluorescence imaging brightness value and the excitation light intensity according to the recorded excitation light intensity and the fluorescence imaging brightness value, wherein the relation formula according to a power dependence formula is as follows: i isF∝Fm(F is excitation light intensity, I)FIs the fluorescence imaging brightness value), and the value of m is obtained according to the formula;
s4: the corresponding exciting light intensity when calculating the luminance reference value of standard sample 3 according to above-mentioned formula, adjust the exciting light intensity that current exciting light intensity corresponds to the luminance reference value, exciting light intensity is the light intensity after passing through the light filter according to light source 7, in the in-service use, light source 7 intensity is adjustable, under the fixed condition of optical density of light filter, exciting light intensity can be adjusted through multiple means, the mode of adjusting exciting light intensity is taken to the angle of rotatory light filter in this embodiment, after adjusting the exciting light intensity that the luminance reference value corresponds, when the image of sample 3 is the clearest this moment, the image quality is high, the experimental data is more accurate.
According to the invention, through the preset brightness reference value, the relation formula between the fluorescence imaging light intensity and the excitation light intensity is automatically calculated, and the light source 7 is automatically adjusted to enable the fluorescence intensity to reach the brightness reference value, so that the analysis of the relation between the fluorescence light intensity and the excitation light intensity is simplified, the manual operation is reduced, the acquisition efficiency is greatly improved, and the image quality of fluorescence imaging is ensured.
Example 2
On the basis of the method for automatically analyzing and adjusting the brightness of the bioluminescence imaging described in the examples, in a preferred embodiment, the step-by-step adjustment of the intensity of the excitation light is specifically as follows: the intensity of the exciting light is adjusted by rotating the continuous variable metal film filter 4 for multiple times to different angles, and the adjustment of the intensity of the exciting light is based on the continuous variable metal film filter 4 in the invention, because the change of the filter is regular, the intensity of the exciting light can be calculated according to the rule.
In a preferred embodiment, the continuously variable metal film filter 4 is controlled by a stepping motor to rotate at different angles, the continuously variable metal film filter 4 is continuously rotated by the steps of the stepping motor during rotation, different attenuation levels are generated for the laser light source 7 by driving the continuously variable metal film filter 4 to rotate at different angles, different excitation light intensities generated by the different attenuation levels are irradiated onto the sample 3, and different steps of the stepping motor rotation and the excitation light intensities corresponding to the different steps are calibrated and recorded into a light flux level table;
in a preferred embodiment, in adjusting the excitation light intensity, the calibration is recorded as a light flux level table by the formula:
excitation light intensity F is 10-ODAnd I (OD is optical density of the optical filter, and I is intensity of the light source 7), a luminous flux level table of which the step number of each stepping motor corresponds to the excitation light intensity is worked out, the step number of the corresponding stepping motor is searched according to the excitation light intensity at the moment, and the luminous flux level table is searched according to the current step number of the stepping motor to obtain the value of the excitation light intensity.
Taking the reflective continuous variable neutral density filter of the metal full-coating film with the optical density range of 0-2.0 and the circular 270 degrees as an example according to the method as follows: the coating range is 90-360 degrees, the optical density of the optical filter linearly increases from the coating start, and the relation between the incident light transmittance and the optical density of the optical filter is T10-OD(T is incident light transmittance, OD is filter optical density), let the incident light intensity be I, the stepping motor step number be N, and the excitation light intensity F be 10-2*((360*n/N-90)/270)I, the calculated luminous flux levels are tabulated in table 1 as follows:
| number of steps of step motor | Intensity of excitation light |
| 1 | I |
| 2 | I |
| … | I |
| n＜(90*N)/360 | I |
| n＞＝(90*N)/360 | 10-2*((360*n/N-90)/270)*I |
| … | … |
| N | I/100 |
TABLE 1
Exciting the relation between light intensity and the number of steps of stepping motor rotation according to the luminous flux level table, and substituting the luminance reference value into formula IF∝FmSince the value of m is already obtained, the excitation light intensity F corresponding to the brightness reference value can be obtained according to the formula, and the corresponding step number of the stepping motor is found through the table of the excitation light intensity and the step number of the stepping motor, for example, n is 2, then in this case, in the luminous flux level table, the value of the excitation light intensity is equal to I.
Example 3
The embodiment provides an automatic analysis and adjustment device for biological fluorescence imaging brightness, which is shown in fig. 2 and adopts one or more methods, and the automatic analysis and adjustment device comprises a continuous variable metal film filter 4 driven to rotate by a driving device 6, wherein a sample 3 and a light source 7 are respectively and correspondingly arranged on two sides of the continuous variable metal film filter 4, a collecting camera 1 is further arranged on the rear side of the sample 3, the collecting camera 1 is connected with a controller 8, and the controller 8 is further connected with a display device 2 and the driving device 6.
In a preferred embodiment, the driving device 6 is a stepping motor, the stepping motor drives the continuous variable metal film filter 4 to rotate through the rotating shaft 5, the continuous variable metal film filter 4 is driven to rotate by different angles to generate different attenuation levels for the laser light source 7, fluorescence imaging is performed by exciting the sample 3 with the exciting light 9 to emit fluorescence 10, the collecting camera 1 collects a fluorescence image of the sample 3 and analyzes fluorescence imaging intensity, the collecting camera 1 usually adopts an industrial camera suitable for the field of fluorescence imaging in specific implementation, combines a preset fluorescence imaging intensity reference value, calculates a brightness difference value between the fluorescence imaging brightness value and a preset brightness reference value through the controller 8, continuously rotates the continuous variable metal film filter 4 with one stepping unit of the stepping motor as a step length, and respectively records the exciting light intensity corresponding to each rotation and the fluorescence imaging brightness value corresponding to each rotation, and fitting a relation formula between the fluorescence imaging brightness value and the intensity of the laser light source 7 by the data, calculating the corresponding excitation light intensity of the sample 3 when the sample 3 sends a reference brightness value according to the formula, and adjusting the excitation light intensity of the light source 7 to the excitation light intensity of the sample 3, thereby obtaining a clear fluorescence image. And the parameters of the relation formula between the fitting fluorescence imaging brightness value and the intensity of the laser light source 7 are displayed through the display device 2, so that the biological fluorescence imaging characteristic analysis is facilitated, and the display device 2 can adopt an LCD, an LED or other liquid crystal display screens.
In the preferred embodiment, the controller 8 may be a computer or an embedded platform such as an ARM, an FPGA, a DSP, etc., but is not limited thereto, and other controllers 8 may be adopted as needed.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (7)
1. The method for automatically analyzing and adjusting the brightness of the biological fluorescence imaging is characterized by comprising the following steps
S1: acquiring a fluorescence imaging brightness value and an excitation light intensity of a current sample, and calculating a brightness difference value according to the fluorescence imaging brightness value and a preset brightness reference value;
s2: adjusting the intensity of the excitation light in a grading manner according to the brightness difference value, adjusting the fluorescence imaging brightness value of the current sample to a brightness reference value, and recording the intensity of the excitation light and the corresponding fluorescence imaging brightness value each time;
s3, fitting a relation formula between the fluorescence imaging brightness value and the excitation light intensity according to the recorded excitation light intensity and the fluorescence imaging brightness value: i isF∝FmWherein F is the intensity of the excitation light, IFThe value of the fluorescence imaging brightness is obtained, and the value of m is obtained according to the formula;
s4: and calculating the excitation light intensity corresponding to the sample fluorescence imaging as the brightness reference value according to the formula, and adjusting the current excitation light intensity to the excitation light intensity corresponding to the brightness reference value, wherein the excitation light intensity is the light intensity of the light source after the light source penetrates through the optical filter.
2. The method for automatically analyzing and adjusting the brightness of bioluminescence imaging according to claim 1, wherein the step-by-step adjustment of the excitation light intensity is specifically: the intensity of the excitation light is adjusted by rotating the continuously variable metal film filter to different angles for a plurality of times.
3. The method for automatically analyzing and adjusting brightness in bioluminescence imaging as claimed in claim 2, wherein the continuous variable metal film filter is controlled by the stepping motor to rotate at different angles, the continuous variable metal film filter is continuously rotated by the number of steps of the stepping motor during the rotation, and since the continuous variable metal film filter is driven to rotate at different angles to generate different attenuation levels for the light source, the different number of steps of the stepping motor rotation and the corresponding excitation light intensity are calibrated and recorded as the light flux level table.
4. The method for automatic analysis and adjustment of brightness in bioluminescence imaging as claimed in claim 3, wherein during calibration recording into a light flux level table, by the formula:
excitation light intensity F =10-OD I, wherein OD is optical density of the optical filter, I is intensity of the light source, and light with excitation light intensity corresponding to the number of steps of each step motor is determinedThe flux level table can search the corresponding step number of the stepping motor through the exciting light intensity at the moment, and can also search the light flux level table through the current step number of the stepping motor to obtain the value of the exciting light intensity.
5. The device for automatically analyzing and adjusting the brightness of biological fluorescence imaging according to any one of claims 1 to 4, comprising a continuous variable metal film filter driven to rotate by a driving device, wherein a sample and a light source are respectively and correspondingly arranged on two sides of the continuous variable metal film filter, a collecting camera is further arranged on the rear side of the sample, the collecting camera is connected with a controller, and the controller is further connected with a display device and the driving device.
6. The device for automatically analyzing and adjusting the brightness of bioluminescence imaging according to claim 5, wherein the driving device is a stepping motor, and the stepping motor drives the continuously variable metal film filter to rotate through a rotating shaft.
7. The device for automatically analyzing and adjusting the brightness of bioluminescence imaging according to claim 6, wherein the controller can be a computer, an ARM, an FPGA or a DSP embedded platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710610519.5A CN107436299B (en) | 2017-07-25 | 2017-07-25 | Method and device for automatically analyzing and adjusting brightness of biological fluorescence imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710610519.5A CN107436299B (en) | 2017-07-25 | 2017-07-25 | Method and device for automatically analyzing and adjusting brightness of biological fluorescence imaging |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107436299A CN107436299A (en) | 2017-12-05 |
| CN107436299B true CN107436299B (en) | 2020-11-27 |
Family
ID=60460130
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710610519.5A Active CN107436299B (en) | 2017-07-25 | 2017-07-25 | Method and device for automatically analyzing and adjusting brightness of biological fluorescence imaging |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107436299B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110361365A (en) * | 2019-02-03 | 2019-10-22 | 北京理工大学 | A kind of scanning fluoroscopic imaging device and the portable QPCR device using it |
| CN112268879B (en) * | 2020-09-17 | 2022-08-26 | 中国科学院生物物理研究所 | Fluorescence microscopic imaging system |
| CN112914477B (en) * | 2021-03-04 | 2023-03-14 | 广东工业大学 | Capsule endoscope system for fluorescence analysis and control method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1428604A (en) * | 2001-12-22 | 2003-07-09 | 浙江大学 | Devide for measuring absolute brightness of fluorescent material |
| CN1162728C (en) * | 1998-12-17 | 2004-08-18 | 莱卡显微系统韦茨拉尔股份有限公司 | Method for dividual adaptation of excitation intensities in multiband fluorescence microscope and multiband fluorescence microscope for carrying out said method |
| CN101788719A (en) * | 2009-12-31 | 2010-07-28 | 华中科技大学 | Method for obtaining continuous luminous energy attenuation ratio smaller than polaroid extinction ratio and attenuator |
| CN102096325A (en) * | 2009-12-10 | 2011-06-15 | 上海微电子装备有限公司 | Light intensity attenuation device and method |
| CN103690137A (en) * | 2014-01-07 | 2014-04-02 | 深圳市开立科技有限公司 | Endoscope light source brightness automatic adjusting method and device |
| CN105223173A (en) * | 2015-09-17 | 2016-01-06 | 天津理工大学 | A kind of fluorescence spectral measuring instrument that local can carry out data manipulation |
| CN106525864A (en) * | 2016-11-22 | 2017-03-22 | 陕西科技大学 | Light source compensation device for image grayscale and compensation method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6571118B1 (en) * | 1998-05-04 | 2003-05-27 | Board Of Regents, The University Of Texas System | Combined fluorescence and reflectance spectroscopy |
| US6906859B2 (en) * | 2002-06-05 | 2005-06-14 | Nikon Corporation | Epi-illumination apparatus for fluorescent observation and fluorescence microscope having the same |
| JP2004163413A (en) * | 2002-08-28 | 2004-06-10 | Carl-Zeiss-Stiftung Trading As Carl Zeiss | Microscope system and microscope inspection method |
| EP2042082A1 (en) * | 2007-09-28 | 2009-04-01 | Stichting Voor De Technische Wetenschappen | Method and apparatus for measuring fluorescence |
| CN104793293A (en) * | 2015-04-30 | 2015-07-22 | 中国医学科学院生物医学工程研究所 | Optical fiber adjusting and fixing device, fluorescent light collecting device and implementing method |
-
2017
- 2017-07-25 CN CN201710610519.5A patent/CN107436299B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1162728C (en) * | 1998-12-17 | 2004-08-18 | 莱卡显微系统韦茨拉尔股份有限公司 | Method for dividual adaptation of excitation intensities in multiband fluorescence microscope and multiband fluorescence microscope for carrying out said method |
| CN1428604A (en) * | 2001-12-22 | 2003-07-09 | 浙江大学 | Devide for measuring absolute brightness of fluorescent material |
| CN102096325A (en) * | 2009-12-10 | 2011-06-15 | 上海微电子装备有限公司 | Light intensity attenuation device and method |
| CN101788719A (en) * | 2009-12-31 | 2010-07-28 | 华中科技大学 | Method for obtaining continuous luminous energy attenuation ratio smaller than polaroid extinction ratio and attenuator |
| CN103690137A (en) * | 2014-01-07 | 2014-04-02 | 深圳市开立科技有限公司 | Endoscope light source brightness automatic adjusting method and device |
| CN105223173A (en) * | 2015-09-17 | 2016-01-06 | 天津理工大学 | A kind of fluorescence spectral measuring instrument that local can carry out data manipulation |
| CN106525864A (en) * | 2016-11-22 | 2017-03-22 | 陕西科技大学 | Light source compensation device for image grayscale and compensation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107436299A (en) | 2017-12-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107436299B (en) | Method and device for automatically analyzing and adjusting brightness of biological fluorescence imaging | |
| Hansen et al. | Robust model-based analysis of single-particle tracking experiments with Spot-On | |
| US20210140866A1 (en) | Rapid Measurement of Formed Blood Component Sedimentation Rate from Small Sample Volumes | |
| JP6031513B2 (en) | Identification and measurement of reticulocytes | |
| JP5185151B2 (en) | Microscope observation system | |
| US20100227316A1 (en) | Luminescense measuring apparatus and luminescense measuring method | |
| CA2718938A1 (en) | Ocular imaging | |
| JP6552041B2 (en) | Microscope system, refractive index calculation method, and program | |
| US10674916B2 (en) | Integrated NIR and visible light scanner for co-registered images of tissues | |
| JP2005043624A (en) | Microscope control unit, microscope system, and microscope objective lens | |
| CN106052866A (en) | Biophoton spectrum detection system and method | |
| CN1826521A (en) | Device for counting micro particles | |
| JP2017504027A (en) | Rapid measurement of blood component sedimentation rates formed from small sample volumes | |
| US20100207036A1 (en) | Optical imaging device | |
| JP2008032440A (en) | Device and method for measuring emission life | |
| Chaiken et al. | Effect of hemoglobin concentration variation on the accuracy and precision of glucose analysis using tissue modulated, noninvasive, in vivo Raman spectroscopy of human blood: a small clinical study | |
| CN107462561B (en) | Fluorescent dynamic automatic detection device and method for tobacco leaf spot character | |
| CN108918523A (en) | Pathology multiple target point intelligent auxiliary diagnosis system | |
| JP2021504679A (en) | Slide rack judgment system | |
| CN105335425A (en) | Fibrosis value assessing method and apparatus and fibrosis dynamic assessing method and system | |
| JP5263744B2 (en) | Photosynthesis activity evaluation program and photosynthesis activity evaluation apparatus | |
| CN106018309A (en) | Multifunctional nucleic acid protein detection system | |
| US7041951B2 (en) | Method and apparatus for investigating layers of tissues in living animals using a microscope | |
| WO2018230318A1 (en) | Scale, image capturing device, image capturing system, kit, and method for adjusting image capturing device | |
| RU2511262C2 (en) | Method for monitoring of treatment of disease involving fluorescence diagnostics of disease, and device for implementing it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |