CN107417402A - A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease and preparation method thereof - Google Patents

A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease and preparation method thereof Download PDF

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Publication number
CN107417402A
CN107417402A CN201710723124.6A CN201710723124A CN107417402A CN 107417402 A CN107417402 A CN 107417402A CN 201710723124 A CN201710723124 A CN 201710723124A CN 107417402 A CN107417402 A CN 107417402A
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parts
preparation
preventing
microbe fertilizer
liquid microbe
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饶霜
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Foshan Sanshui Charles Agricultural Technology Research Institute (general Partner)
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Foshan Sanshui Charles Agricultural Technology Research Institute (general Partner)
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C05G5/27—Dispersions, e.g. suspensions or emulsions

Abstract

The present invention relates to a kind of liquid microbe fertilizer and preparation method thereof for preventing and treating tomato fungal disease, include the raw material of following weight parts：5~25 parts of streptomyces microflavus zymotic fluid, 3~12 parts of calcium silicates, 5~17 parts of epsom salt, 0.5~1.5 part of ferric sulfate, 0.3~1.0 part of borax, 0.3~0.5 part of zinc sulfate；Its preparation method prepares seaweed extracted liquor including ultrasonic wave added enzymolysis, the seaweed extracted liquor of preparation is configured to fermentation medium again, streptomyces microflavus is cultivated under certain condition, and finally by zymotic fluid and middle trace element built, liquid microbe fertilizer of the invention is made.Gained liquid microbe fertilizer of the invention can not only effectively improve utilization rate of fertilizer, promote absorption, operating and the use of tomato nutrient material, tomato disease resistance can also be improved, prevent and treat fungal disease, improve Tomato Quality, tomato yield is improved, is to integrate nutrition, volume increase, disease-resistant fully natural green fertilizer.

Description

A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease and preparation method thereof
Technical field
The invention belongs to microbial manure technical field, and in particular to a kind of liquid microbe for preventing and treating tomato fungal disease Fertilizer and preparation method thereof.
Background technology
Tomato is that Solanaceae tomato genus is annual or herbaceos perennial, Peru, strategic point originating from South America western part plateau The country such as melon Dorr and Bolivia.Tomato both can be described as vegetables, also referred to as fruit, containing abundant vitamin and mineral, Particularly vitamin C and lycopene content highest, there is very high nutritive value.In recent years, with cultivation of the tomato in China Training area constantly expands, and tomato disease also aggravates increasingly, wherein several local infection venereal diseases do harm to, such as gray mold, leaf mold, early epidemic Disease, late blight, powdery mildew, droop etc., occurrence frequency is high, then infects often, popular strong, it is difficult to prevent and treat, made to production Into heavy losses.Traditional prevention and controls, such as chemical pesticide or the abuse of bactericide, caused to China's Agro-ecological System Have a strong impact on, not only plant is influenceed by poisoning, directly hinder its growth, can also cause nuisance or residues of pesticides, Environment is polluted, can more make the increase of the pathogen resistance to the action of a drug, secondly, several plant source bactericide few in number is also set because it is complicated The reasons such as standby, smart sharp technology, high cost, and be difficult to widely be promoted.Therefore, there is an urgent need to a kind of high now Effect, safely, conveniently and " three-lows " prophylactico-therapeutic measures, by the damage control caused by disease in floor level.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of liquid microbe for preventing and treating tomato fungal disease Fertilizer, effectively solves that tomato harmful substance or residues of pesticides, pathogen are developed immunity to drugs in the prior art and environmental pollution etc. is asked Topic, and the validity of nutrient and moisture can be effectively improved, promote absorption, operating and the use of tomato nutrient material, improve tomato Disease resistance, improve Tomato Quality, improve tomato yield, be to integrate nutrition, volume increase, disease-resistant fully natural green fertilizer.
The present invention is achieved through the following technical solutions：
A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, include the raw material of following weight parts：Thin yellow strepto- 5~25 parts of fermented liquid, 3~12 parts of calcium silicates, 5~17 parts of epsom salt, 0.5~1.5 part of ferric sulfate, borax 0.3~1.0 Part, 0.3~0.5 part of zinc sulfate.
Preferably, a kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, the raw material of following weight parts are included：Carefully 10~20 parts of yellow streptomycete zymotic fluid, 5~10 parts of calcium silicates, 7~13 parts of epsom salt, 0.8~1.3 part of ferric sulfate, borax 0.3~0.8 part, 0.3~0.5 part of zinc sulfate.
Preferably, a kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, the raw material of following weight parts are included：Carefully 18 parts of yellow streptomycete zymotic fluid, 8 parts of calcium silicates, 11 parts of epsom salt, 1.0 parts of ferric sulfate, 0.6 part of borax, zinc sulfate 0.4 Part.
The present invention also provides a kind of preparation method for the liquid microbe fertilizer for preventing and treating tomato fungal disease, including following step Suddenly：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：30~60 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 Amount adds deionized water, heats enzyme deactivation, obtains suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral protein Enzyme, cellulase, non-starch polysaccharide enzyme, digest under ultrasonic assistant effect, during which at the uniform velocity stirred using mixer；Enzymolysis knot Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor after beam；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A) Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1~3%, 1~2%, 0.01~0.05%, 0.02~0.05%, 0.001~0.005%, pH is adjusted to 7.0~7.4；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), after inoculation activation Streptomyces microflavus, oscillation and fermentation cultivation, in obtained zymotic fluid the CFU of streptomyces microflavus for streptomyces microflavus >= 2.0×109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Thin yellow strepto- obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (1) is weighed by weight Fermented liquid, be well mixed, both liquid microbe fertilizer of the present invention.
Preferably, α~amylase in step (1), neutral proteinase, cellulase, non-starch polysaccharide enzyme addition according to It is secondary for 3~5%, 1~2%, 2~3%, the 1~2% of marine alga quality.
Preferably, enzymatic hydrolysis condition is in step (1)：50~70 DEG C, ultrasonic power 300W of hydrolysis temperature, enzymolysis time 30 ~60min.
Preferably, streptomyces microflavus inoculation percentage by weight is 1%~5% in step (1).
Preferably, fermentation culture conditions are in step (1)：28~32 DEG C, 4~6d of incubation time of cultivation temperature, vibration training It is 160~260rpm/min to support rotating speed.
Each composition mechanism of action of the present invention is as follows：
Marine alga：Marine alga is the cryptogam of plant kingdom, and species is various, abundance.According to contained pigment in marine alga not Together, green alga, brown alga, blue-green algae, xanthophyta, chrysophyceae etc. can be classified as.Marine alga is rich in algal polysaccharides, the dirty acid of algae, amino acid, inorganic The physiological activator such as salt, vitamin and a small amount of enzyme, various plants hormone, polyphenol, plant growth can be induced and reduced non- The mechanism of biotic, the defence capability and osmotic protection function of crop are excited, and it is nontoxic pollution-free, it is pollution-free food Preferred fertilizer.Secondly, alginic acid contained in marine alga, has the function that complexation of metal ions, and itself and middle trace element are coordinated Using nutrient can be kept, fertilizer loss is reduced, improves utilization rate of fertilizer.
Streptomyces microflavus：Streptomyces microflavus is a kind of resourceful actinomyces, can produce netropsin, spiramvcin etc. Antibacterial material, suppress Gram-positive and negative bacteria, saccharomycete, the growth and breeding of filamentous fungi, promote plant growth, prevention Disease occurs.Research shows that streptomyces microflavus shows to pathogen of crop such as downy mildew of alfalfa, powdery mildew, brown spot, root rot Good prevention effect is shown, the upper diseases prevention that is often used in agriculture is kept a full stand of seedings；Streptomyces microflavus also acts as bacterial manure, converts nitrogen phosphorus in soil Element, increase soil fertility, and play the role of to stimulate plant growth.
Silicon：Silicon is the important component of plant, is the important nutrient that plant needs.Silicon is mainly deposited on blade And leaf sheath epidermal cell, form silicified cell and cutin-silicon double-decker, can strengthen host plant cell wall mechanical strength and Steadiness, so as to delay and resist the intrusion of germ and extension.Silicon processing can also dramatically increase plant leaf blade protective enzyme (peroxidating Thing enzyme, polyphenol oxidase, phenylalanine lyase etc.) active and induction host's generation secondary metabolism resisting substance (such as protective plant protecting agent, Polyphenol compound, lignin), so as to the system of defense of activated plant, strengthen the resistivity to pathogen.Secondly, silicon is also Absorption of the plant to mineral nutrition can be improved, promote the robust growth of plant, enhancing plant drought, disease-resistant, pest-resistant, salt resistance, The degeneration-resistant effect such as freeze proof, anti-metallic contamination, resistant to lodging, improve quality of agricultural product, be advantageous to store and transport.
Calcium：Calcium is the constituent of cell membrane mesoglea, enables cell to be tied to form tissue with cell, and make plant Organ or individual there is certain mechanical strength；Secondly, calcium has a certain impact to nitrogen metabolism in crop body, and can neutralize work The organic acid formed in thing metabolic process, to stablize intracellular environment play the role of it is important；Calcium can also lower plasm colloid Decentralization, water-filling degree, viscosity, elasticity and permeability of cell etc. is maintained normal physiological status, be advantageous to crop just Often metabolism.Tomato genus likes the vegetable crop of calcium, reacts sensitive to calcium, and during tomato calcium deficiency, plant is thin and weak, and root system is undeveloped, and meeting Induce physiological disturbance, such as blossom-end rot.
Magnesium：Magnesium is the constituent of plant chlorophyll and polysome, and the activator of a variety of enzymes, can promote to make object Interior vitamin A, ascorbic formation.Therefore, the synthesis of the photosynthesis and protein of crops can be promoted using fertiliser containing magnesium, carried High crop yield, improve quality of agricultural product.According to another report, magnesium can also promote absorption of the plant to silicon, be advantageous to prevent germ Intrusion.
Iron：Iron participates in the synthesis of chlorophyll, and it is the composition of some enzymes of composition and protein, participates in the oxygen in plant The manufacture of change-reduction process and carbohydrate.
Boron：Boron is the constituent of a variety of enzymes of plant, participates in the generation of the synthesis of plant vivo protein, nitrogen and carbohydrate Thank.Boron can promote the normal operation of carbohydrate and the normal development of reproductive organs, improve the supply of oxygen, the development to root system And the formation of fruit, seed has a major impact.Secondly, boron can also adjust absorption and oxidation-reduction process of the plant to moisture, Also the formation of Legume nodule can be promoted.
Zinc：Zinc be growth and development of plants necessary to trace element, be many enzymes in plant constituent and activator, The formation of auximone is directly participated in, Substance P hydrolysis, oxidation-reduction process in plant are played an important role, can be also catalyzed Protein synthesizes, and promotes seed maturity etc..
Beneficial effects of the present invention：
1st, the present invention prepares seaweed extracted liquor using the method for ultrasonic wave added enzymolysis, avoids strong in marine alga process The destruction of alkali, high temperature, high pressure, the nutriment enriched in marine alga is fully retained；
2nd, the present invention digests ultrasonic wave added the fermented and cultured that the seaweed extracted liquor prepared is used for streptomyces microflavus, gained hair Zymotic fluid has marine alga activity and the live body regulatory function of streptomyces microflavus concurrently, and it is active organic with bacteria agent to realize seaweed bio It is unified；
3rd, the present invention not only can effectively carry streptomyces microflavus zymotic fluid and middle trace element built, gained liquid fertilizer High utilization rate of fertilizer, promote absorption, operating and the use of tomato nutrient material, can also improve tomato disease resistance, preventing and treating blade face disease Evil, improve Tomato Quality, improve tomato yield, be to integrate nutrition, volume increase, disease-resistant fully natural green fertilizer.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
Embodiment 1
A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, include the raw material of following weight parts：Thin yellow strepto- 5 parts of fermented liquid, 12 parts of calcium silicates, 17 parts of epsom salt, 0.5 part of ferric sulfate, 0.3 part of borax, 0.5 part of zinc sulfate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating tomato fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：60 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 3%, 2%, 3%, the 1% of marine alga quality, is acted in ultrasonic assistant Lower enzymolysis, 50 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 60min, is during which at the uniform velocity stirred using mixer；Enzymolysis Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor after end；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A) Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1%, 2%, 0.01%, 0.05%, 0.001%, pH are adjusted to 7.0；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with Than the streptomyces microflavus after the activation for 5%, the thin yellow chain in 32 DEG C, 160rpm/min shaken cultivation 6d, obtained zymotic fluid The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Thin yellow strepto- obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (1) is weighed by weight Fermented liquid, be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 2
A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, include the raw material of following weight parts：Thin yellow strepto- 20 parts of fermented liquid, 5 parts of calcium silicates, 13 parts of epsom salt, 0.8 part of ferric sulfate, 0.3 part of borax, 0.5 part of zinc sulfate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating tomato fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：40 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 4.5%, 1.8%, 2.7%, the 1.7% of marine alga quality, in ultrasonic wave Digest, 65 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 45min, during which at the uniform velocity stirred using mixer under booster action Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A) Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1.5%, 1.8%, 0.02%, 0.04%, 0.004%, pH are adjusted to 7.1；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with Than the streptomyces microflavus after the activation for 4%, the thin yellow chain in 30 DEG C, 180rpm/min shaken cultivation 6d, obtained zymotic fluid The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Thin yellow strepto- obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (1) is weighed by weight Fermented liquid, be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 3
A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, include the raw material of following weight parts：Thin yellow strepto- 18 parts of fermented liquid, 8 parts of calcium silicates, 11 parts of epsom salt, 1.0 parts of ferric sulfate, 0.6 part of borax, 0.4 part of zinc sulfate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating tomato fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：47 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 4%, 1.5%, 2.5%, the 1.5% of marine alga quality, auxiliary in ultrasonic wave Help effect is lower to digest, 60 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 40min, during which at the uniform velocity stirred using mixer Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A) Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 2%, 1.5%, 0.03%, 0.03%, 0.003%, pH are adjusted to 7.3；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with Than the streptomyces microflavus after the activation for 3%, the thin yellow chain in 29 DEG C, 230rpm/min shaken cultivation 5d, obtained zymotic fluid The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Thin yellow strepto- obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (1) is weighed by weight Fermented liquid, be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 4
A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, include the raw material of following weight parts：Thin yellow strepto- 25 parts of fermented liquid, 3 parts of calcium silicates, 5 parts of epsom salt, 1.5 parts of ferric sulfate, 1.0 parts of borax, 0.3 part of zinc sulfate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating tomato fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：30 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 3.5%, 1.3%, 2.3%, the 1.3% of marine alga quality, in ultrasonic wave Digest, 55 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 50min, during which at the uniform velocity stirred using mixer under booster action Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A) Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 2.5%, 1.3%, 0.04%, 0.03%, 0.002%, pH are adjusted to 7.4；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with Than the streptomyces microflavus after the activation for 3%, the thin yellow chain in 27 DEG C, 200rpm/min shaken cultivation 5d, obtained zymotic fluid The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Thin yellow strepto- obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (1) is weighed by weight Fermented liquid, be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 5
A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease, include the raw material of following weight parts：Thin yellow strepto- 10 parts of fermented liquid, 10 parts of calcium silicates, 7 parts of epsom salt, 1.3 parts of ferric sulfate, 0.8 part of borax, 0.3 part of zinc sulfate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating tomato fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：50 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 5%, 1%, 2%, the 2% of marine alga quality, is acted in ultrasonic assistant Lower enzymolysis, 70 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 30min, is during which at the uniform velocity stirred using mixer；Enzymolysis Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor after end；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A) Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 3%, 1%, 0.05%, 0.02%, 0.005%, pH are adjusted to 7.2；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with Than the streptomyces microflavus after the activation for 3%, the thin yellow chain in 28 DEG C, 260rpm/min shaken cultivation 4d, obtained zymotic fluid The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Thin yellow strepto- obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (1) is weighed by weight Fermented liquid, be well mixed, both liquid microbe fertilizer of the present invention.
Comparative example 1
Streptomyces microflavus is not added in liquid fertilizer, includes the raw material of following weight parts：8 parts of calcium silicates, seven water sulfuric acid 11 parts of magnesium, 1.0 parts of ferric sulfate, 0.6 part of borax, 0.4 part of zinc sulfate.
The preparation method of aforesaid liquid fertilizer, comprises the following steps：
(1) preparation of seaweed extracted liquor：47 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 4%, 1.5%, 2.5%, the 1.5% of marine alga quality, auxiliary in ultrasonic wave Help effect is lower to digest, 60 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 40min, during which at the uniform velocity stirred using mixer Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
(2) glucose, yeast juice, potassium dihydrogen phosphate, sodium chloride, sulfuric acid are added into seaweed extracted liquor obtained by step (1) Ferrous iron, addition be respectively seaweed extracted liquor quality 3%, 1%, 0.05%, 0.02%, 0.005%, pH be adjusted to 7.2, obtain mixed Close liquid A；
(3) weigh and mixed obtained by calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and step (2) by weight Liquid A, be well mixed, both liquid fertilizer of the present invention.
Test example 1
The measure of technical indicator
1st, test objective：Detect effective viable bacteria in a kind of liquid microbe fertilizer of preventing and treating tomato fungal disease of the present invention Number, miscellaneous bacteria rate, pH and innoxious index.
2nd, sample is detected：The fertilizer sample prepared by above-described embodiment 1~5 and comparative example 1.
3rd, detection method：Detected according to the national standard (GB20287-2006) of microbial manure.
4th, testing result：Testing result is as shown in table 1 below.
The technical indicator measurement result of table 1
5th, conclusion
After testing, a kind of liquid microbe fertilizer of preventing and treating tomato fungal disease of the invention meets the state of microbial manure Family's standard (GB20287-2006).Compared with comparative example 1, living bacteria count dramatically increases in embodiments of the invention 1~5, miscellaneous Bacterium rate significantly reduces, and living bacteria count highest contained in embodiment 3, is 5.5 hundred million/milliliter, and miscellaneous bacteria rate is minimum, is 3.2%. Living bacteria count is the main index of microbial manure product, illustrates that a kind of liquid of preventing and treating tomato fungal disease of the present invention is micro- Bio-feritlizer is had fine qualities, and the yield and quality of agricultural product can be significantly improved for agricultural production.
Test example 2
A kind of inhibitory action of the liquid microbe fertilizer of preventing and treating tomato fungal disease of the present invention to tomato disease fungus
1st, test objective：The liquid microbe fertilizer of the present invention is detected to 5 kinds of inhibitory action for examination tomato disease fungus.
2nd, sample is detected：The fertilizer sample prepared by above-described embodiment 1~5 and comparative example 1.
3rd, for trying pathogen：Ash arrhizus bacteria, Fulvia fulva Ciferri, early epidemic germ, Powdery Mildew, wilt.
4th, assay method and step：
Fertilizer sample is determined to 5 kinds of inhibitory action for examination pathogen using mycelial growth rate method.Fertilizer sample is used Aseptic deionized water is diluted to 30,60,100,150 times, is added to and 50 DEG C or so of PDA culture medium is cooled to after quantitative sterilizing In, fully shake up, drug containing flat board is made.To add with sample equivalent aseptic deionized water to compare, often processing is repeated 3 times.With straight Footpath 5mm card punch, which is beaten, takes well-grown stem, and stem mycelia is inoculated in into drug containing flat board center downwards.After connecting strain 25 DEG C of constant incubator culture a couple of days are positioned over, measure colony diameter, calculate mycelial growth inhibition rate.Checked by the 3rd day As a result, stop checking when bacterium colony length to plate edge is compareed.Bacteriostasis rate is calculated by following formula.
Bacteriostasis rate=[(control colony diameter-processing colony diameter)/(control colony diameter -5)] × 100%
5th, measurement result：Testing result is as shown in table 2 below.
Table 2 is to the inhibitory action for trying pathogen
6th, conclusion
After testing, the liquid microbe fertilizer dilution of a kind of preventing and treating tomato fungal disease prepared by the embodiment of the present invention 1~5 During to 30 times, 60 times, the bacteriostasis rate of ash arrhizus bacteria, Fulvia fulva Ciferri, early epidemic germ, Powdery Mildew and wilt is reached 100%, when fertilizer sample is diluted to 100 times, 150 times, to the bacteriostasis rate of ash arrhizus bacteria, Fulvia fulva Ciferri and Powdery Mildew compared with Height, reach more than 86.2%.
Result of the test shows that a kind of liquid microbe fertilizer of preventing and treating tomato fungal disease of the invention can be prevented and treated effectively Fungal disease, have a extensive future.
Test example 3
A kind of influence of the liquid microbe fertilizer of preventing and treating tomato fungal disease of the present invention to tomato yield and quality
1st, experimental design
For studying thing to grind short Fructus Lycopersici esculenti kind together, planting density is 4000 plants/hm2；Set two processing altogether, 3 repetitions, Plot area is 30m2, random district's groups arrangement.Each processing sets as follows：
Handle A：Habits fertilization, composite fertilizer (N-P2O5-K2O=15-15-15) 50kg/ mus；
Handle B：The liquid microbe fertilizer prepared by above-described embodiment 3 carries out foliage-spray or pouring root, composite fertilizer (N- P2O5-K2O=15-15-15) 50kg/ mus.
2nd, fertilization time and method
When site preparation applies fertilizer to the subsoil, applied by every mu of 50kg composite fertilizer for trying in soil, then ridging.Choose growing way uniform one The healthy seedling of cause is unified to be colonized.The liquid that processing B respectively sprays in Tomato during Flower Initiation Stage, first fruiting period, the best fruiting period or prepared by pouring root embodiment 3 Once, the initial bloom stage amount of spraying or pouring root amount are 2kg/ mus to body microbial manure, and the first fruiting period amount of spraying or pouring root amount is 4kg/ mus, are contained The fruiting period amount of spraying or pouring root amount are 8kg/ mus.The conditions such as experimental plot others base manure, top dressing, irrigation and other field management are equal Unanimously.
3rd, pilot survey
Harvest and sample in the tomato best fruiting period, and determine each processing tomato yield (single fruit weight, single plant yield and cell production) And quality (vitamin C, reduced sugar and titrable acidity).Sample collection is that 20~30 plants are investigated per cell, is averaged.
4th, result of the test：As a result it is as shown in table 3 below.
Influence of the table 3 to tomato yield and quality
5th, conclusion
By the investigation statisticses to tomato yield and quality, using a kind of preventing and treating tomato fungal disease of the invention Liquid microbe fertilizer can be effectively increased tomato yield, improve Tomato Quality.Compared with habits fertilization, processing B single fruit weights, individual plant Yield and cell production add 18.80%, 51.99%, 28.42% respectively, and vitamin C and reduction sugar amount add respectively 36.14% and 29.64%, titrable acidity reduces 33.48%.
Vitamin C, reduced sugar and titrable acidity are the important indicators of tamato fruit nutritional quality, and the height of its content is determined Tomato nutrient value and the quality of taste are determined, so as to influence the commodity value of tomato.Shown by the comparison of data above, A kind of liquid microbe fertilizer of preventing and treating tomato fungal disease of the present invention can be effectively increased vitamin C and reduction in tamato fruit Sugared content, titratable acid content is reduced, Tomato Quality can be effectively improved by illustrating the liquid microbe fertilizer of the present invention, improve tomato Nutritive value, has important society and economic value, and market development has a extensive future.

Claims (8)

  1. A kind of 1. liquid microbe fertilizer for preventing and treating tomato fungal disease, it is characterised in that the raw material including following weight parts： 5~25 parts of streptomyces microflavus zymotic fluid, 3~12 parts of calcium silicates, 5~17 parts of epsom salt, 0.5~1.5 part of ferric sulfate, borax 0.3~1.0 part, 0.3~0.5 part of zinc sulfate.
  2. A kind of 2. liquid microbe fertilizer for preventing and treating tomato fungal disease according to claim 1, it is characterised in that including The raw material of following weight parts：10~20 parts of streptomyces microflavus zymotic fluid, 5~10 parts of calcium silicates, 7~13 parts of epsom salt, 0.8~1.3 part of ferric sulfate, 0.3~0.8 part of borax, 0.3~0.5 part of zinc sulfate.
  3. A kind of 3. liquid microbe fertilizer for preventing and treating tomato fungal disease according to claim 2, it is characterised in that including The raw material of following weight parts：18 parts of streptomyces microflavus zymotic fluid, 8 parts of calcium silicates, 11 parts of epsom salt, 1.0 parts of ferric sulfate, 0.6 part of borax, 0.4 part of zinc sulfate.
  4. A kind of a kind of 4. system of the liquid microbe fertilizer of preventing and treating tomato fungal disease as described in any one of claims 1 to 3 Preparation Method, comprise the following steps：
    (1) preparation of streptomyces microflavus zymotic fluid：
    A, the preparation of seaweed extracted liquor：30~60 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre Plain enzyme, non-starch polysaccharide enzyme are tieed up, digests under ultrasonic assistant effect, is during which at the uniform velocity stirred using mixer；After enzymolysis terminates Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor；
    B, the preparation of fluid nutrient medium：Added into seaweed extracted liquor obtained by step (A) glucose, yeast juice, potassium dihydrogen phosphate, Sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1~3%, 1~2%, 0.01~0.05%, 0.02~ 0.05%th, 0.001~0.005%, pH is adjusted to 7.0~7.4；
    C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), the thin Huang after inoculation activation Streptomycete, oscillation and fermentation cultivation, in obtained zymotic fluid the CFU of streptomyces microflavus for streptomyces microflavus >=2.0 × 109cfu/mL；
    (2) preparation of liquid microbe fertilizer：
    Calcium silicates, epsom salt, ferric sulfate, borax, zinc sulfate and streptomyces microflavus hair obtained by step (1) are weighed by weight Zymotic fluid, it is well mixed, produces liquid microbe fertilizer of the present invention.
  5. 5. a kind of preparation method of liquid microbe fertilizer for preventing and treating tomato fungal disease according to claim 4, it is special Sign is that α~amylase, neutral proteinase, cellulase, the addition of non-starch polysaccharide enzyme are followed successively by the step (1) 3~5%, 1~2%, 2~3%, the 1~2% of marine alga quality.
  6. 6. a kind of preparation method of liquid microbe fertilizer for preventing and treating tomato fungal disease according to claim 4, it is special Sign is that enzymatic hydrolysis condition is in the step (1)：50~70 DEG C, ultrasonic power 300W of hydrolysis temperature, enzymolysis time be 30~ 60min。
  7. 7. a kind of preparation method of liquid microbe fertilizer for preventing and treating tomato fungal disease according to claim 4, it is special Sign is that streptomyces microflavus inoculation percentage by weight is 1%~5% in the step (1).
  8. 8. a kind of preparation method of liquid microbe fertilizer for preventing and treating tomato fungal disease according to claim 4, it is special Sign is that fermentation culture conditions are in the step (1)：28~32 DEG C, 4~6d of incubation time of cultivation temperature, shaken cultivation turn Speed is 160~260rpm/min.
CN201710723124.6A 2017-08-21 2017-08-21 A kind of liquid microbe fertilizer for preventing and treating tomato fungal disease and preparation method thereof Pending CN107417402A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108947685A (en) * 2018-08-22 2018-12-07 中国热带农业科学院热带作物品种资源研究所 The nutrient compositions for preventing and treating capsicum epidemic disease
CN112592239A (en) * 2020-02-06 2021-04-02 山东世纪瑞文信息技术有限公司 Seaweed calcium magnesium fertilizer and preparation method thereof
CN114410518A (en) * 2022-01-07 2022-04-29 春华秋实科技集团有限公司 Compound microbial agent containing streptomyces and application thereof
US11521381B2 (en) * 2019-05-13 2022-12-06 Bao Tran Smart farming
CN116874321A (en) * 2023-07-14 2023-10-13 广州优卡思农业技术有限公司 Method for preparing seaweed liquid fertilizer by using seaweed

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106866270A (en) * 2017-03-25 2017-06-20 广州聚禅现代农业研究院有限公司 A kind of liquid microbe fertilizer of the changing food waste into resources production containing streptomyces microflavus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106866270A (en) * 2017-03-25 2017-06-20 广州聚禅现代农业研究院有限公司 A kind of liquid microbe fertilizer of the changing food waste into resources production containing streptomyces microflavus

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108947685A (en) * 2018-08-22 2018-12-07 中国热带农业科学院热带作物品种资源研究所 The nutrient compositions for preventing and treating capsicum epidemic disease
CN108947685B (en) * 2018-08-22 2021-03-23 中国热带农业科学院热带作物品种资源研究所 Nutrient element composition for preventing and treating pepper phytophthora blight
US11521381B2 (en) * 2019-05-13 2022-12-06 Bao Tran Smart farming
CN112592239A (en) * 2020-02-06 2021-04-02 山东世纪瑞文信息技术有限公司 Seaweed calcium magnesium fertilizer and preparation method thereof
CN114410518A (en) * 2022-01-07 2022-04-29 春华秋实科技集团有限公司 Compound microbial agent containing streptomyces and application thereof
CN116874321A (en) * 2023-07-14 2023-10-13 广州优卡思农业技术有限公司 Method for preparing seaweed liquid fertilizer by using seaweed

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