CN106811433A - A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect - Google Patents
A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect Download PDFInfo
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- CN106811433A CN106811433A CN201710098015.XA CN201710098015A CN106811433A CN 106811433 A CN106811433 A CN 106811433A CN 201710098015 A CN201710098015 A CN 201710098015A CN 106811433 A CN106811433 A CN 106811433A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Abstract
The present invention relates to a kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect, it is made up of the microbial fermentation solution of following parts by volume：1~10 part of blown-ball Azotobacter, 1~30 part of gel-shaped bacillus, 1~10 part of phosphorus decomposing bacillus megaterium, 1~10 part of bacillus amyloliquefaciens, 1~10 part of bacillus subtilis, 1~5 part of photosynthetic bacteria, 1~10 part of streptomyces microflavus.The present invention selects above-mentioned functions bacterium as the major function flora of microbial manure, products obtained therefrom not only has significant fixing nitrogen, dissolving phosphor and dissolving potassium and disease-resistant effect, soil bacterial community total metabolism activity and community function diversity can also be strengthened, improve soil microenvironment, promote soil beneficial microorganism growth and breeding, suppress growth of pathogenic bacteria, be the preferable fertilizer of green agriculture, organic agriculture, there are bright prospects in agricultural sustainable development.
Description
Technical field
The invention belongs to fertilizer field, and in particular to a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect and
Preparation method.
Background technology
Microbial manure, also known as bio-feritlizer, bacterial manure or Inoculant, is that a class is led with microbial life activity and its product
Crops are caused to obtain the living microorganisms product of specific fertilizer effect.Microbial manure culture fertility, improve chemical fertilizer utilization ratio,
Suppress crops to nitrate nitrogen, heavy metal, agricultural chemicals absorption, purification and rehabilitating soil, reduce corps diseases occur, promote agriculture
Crop material becomes thoroughly decomposed utilization, protects the fields and environment and improves crop products quality and food security aspect is demonstrated by replacing
The effect in generation, is the key of agricultural production sustainable development synchronous with ecological environment.
Strain is the core of microbial manure product, is also the key of product effect.However, single fertilizer amendments bacterium
Planting can only often provide single nutrient or simple function, and in agricultural production practice, N P and K and other moderate-elements need to put down
Weighing apparatus coordinates supply, therefore, multi-cultur es compounding is one of underlying issue of microbial manure research.
The A of Chinese patent application 103613441, discloses a kind of composite microbe fertilizer and preparation method thereof, the composite microbial
Thing fertilizer by by molasses, wheat bran, filter mud of sugarcane, tobacco powder, medical stone, bacterium of becoming thoroughly decomposed, fixing nitrogen, dissolving phosphor and dissolving potassium strain and bentonite in hair
After fermenting pot 30 days, wet thallus are collected, then obtain, wet thallus high-temperature sterilization in the mistake of high-temperature sterilization through granulating and drying
Cheng Zhong, fixing nitrogen, dissolving phosphor and dissolving potassium strain is also inactivated simultaneously, therefore, obtained microorganism fertilizer is without fixing nitrogen, dissolving phosphor and dissolving potassium strain etc.
Beneficial microbe, product itself does not possess effect of fixing nitrogen, dissolving phosphor and dissolving potassium.The A of Chinese patent application 1331061, discloses one kind
Microbial manure with nitrogen-fixating and P and K decomposing functions, the microbial manure function bacterium is Bacillus macerans, huge gemma bar
Bacterium, colloid bacillus cereus, have functions that fixed nitrogen, phosphorus decomposing, potassium decomposing and promote plant strain growth, but the microbial manure not to add
Suppress the microorganism of plant growth of pathogenic bacteria breeding, it is impossible to direct effectively prevention and control plant diseases.
The content of the invention
It is an object of the present invention to meet the market demand, solve above-mentioned technological deficiency, there is provided one kind has fixed nitrogen phosphorus decomposing
Liquid microbe fertilizer of potassium decomposing and disease-resistant effect and preparation method thereof.
The present invention is achieved through the following technical solutions：
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, by the parts by volume group of following microbial fermentation solution
Into：1~10 part of blown-ball Azotobacter, 3~30 parts of gel-shaped bacillus, 1~10 part of phosphorus decomposing bacillus megaterium, solution starch gemma
1~10 part of bacillus, 1~10 part of bacillus subtilis, 0.5~5 part of photosynthetic bacteria, 1~10 part of streptomyces microflavus；
Preferably, a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, by following microbial fermentation solution
Parts by volume is constituted：3~8 parts of blown-ball Azotobacter, 9~24 parts of gel-shaped bacillus, 3~8 parts of phosphorus decomposing bacillus megaterium, Xie Dian
3~8 parts of afnyloliquefaciens, 3~8 parts of bacillus subtilis, 1.5~4 parts of photosynthetic bacteria, 3~8 parts of streptomyces microflavus；
Preferably, a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, by following microbial fermentation solution
Parts by volume is constituted：6 parts of blown-ball Azotobacter, 18 parts of gel-shaped bacillus, 6 parts of phosphorus decomposing bacillus megaterium, solution starch gemma bar
6 parts of bacterium, 6 parts of bacillus subtilis, 3 parts of photosynthetic bacteria, 6 parts of streptomyces microflavus；
Preferably, a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, the photosynthetic bacteria is selected from marsh
Red pseudomonas, plus black red pseudomonas or Rhodopseudomonas spheroides in one or more.
Preferably, a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, be applied to paddy rice, vegetables, melon and fruit,
Tobacco, cotton, flowers, the plantation of Chinese herbal medicine.
Each function bacterium mechanism of action of the present invention is as follows：
Blown-ball Azotobacter：Blown-ball Azotobacter belongs to itself nitrogen-fixing bacteria, and it is obligate aerobic to be that a class can independently be carried out in soil
The bacterium of fixed nitrogen activity, there is important value in terms of microbial manure.Blown-ball Azotobacter can be aobvious by nitrogen fixation
Writing increases the soil organism and total nitrogen content, improves soil physico-chemical property, and the total metabolism of enhancing plant rhizosphere soil bacterial community is lived
Property and community function diversity, promote plant strain growth, improve quality of vegetable.Its mechanism of action includes：(1) blown-ball Azotobacter metabolism
Product can promote the growth of other beneficial microbe monoids of soil；(2) blown-ball Azotobacter can promote root system of plant biomass
Improve and root system bifurcated number increases, and then cause that root activity increases, root exudates increases (such as carbohydrate, amino acid and many
Plant trace element), accelerate Soil Nutrient Transformation.Now, the available existence attachment space of other bacteriums in Rhizosphere environment
(root system of plant) and energy matter (plant residue or root exudates, the protein matter of mycelia secretion) are significantly improved, from
And bacterial number increases, increased activity.
Gel-shaped bacillus：Gel-shaped bacillus, is commonly called as B. mucilaginocus, also known as silicate bacteria, is common in farmland
A kind of soil bacteria, its as plant growth-promoting rhizobacteria to phosphorus potassium mineral have utilization and capacity of decomposition, potassium can be discharged, increase
Plus the content of soil quick-effective phosphor, alkali-hydrolyzable nitrogen and available potassium, strengthen soil enzyme activities, so as to improve soil microenvironment, it is beneficial to
Beneficial microbe and the growth of plant.Gel-shaped bacillus can also produce the materials such as organic acid, amino acid, hormone, promote to plant
Strain growth, effectively improves plant quality.
Phosphorus decomposing bacillus megaterium：Phosphorus decomposing bacillus megaterium is that a kind of phosphorus decomposing promotees B. mucilaginocus, can produce corydalis tuber, breast
The organic acids such as acid, hydroxyacetic acid and butanedioic acid, these organic acids can reduce pH value, be combined with aluminium, iron, magnesium, calcium plasma,
The phosphate of slightly solubility is dissolved, the ADSORPTION STATE organophosphor that cannot be directly utilized to plant in lecithin, soil, Phos have substantially
Decomposition, and can increase soil fertility, promote the volume increase of crop；There is synergy to make fixed nitrogen when being mixed with nitrogen-fixing bacteria
With.
Bacillus amyloliquefaciens：Bacillus amyloliquefaciens are a kind of and bacillus subtilis affinity bacterium very high,
A series of metabolite, amount of activated material can be produced in the growth course of its own can effectively suppress phytopathogen
Growth and breeding, such as low-molecular-weight antibiotic and antibacterial protein or polypeptide；Amount of activated material can remarkably promote plant
Growth, such as gibberellin, heteroauxin, the basic element of cell division.Secondly, bacillus amyloliquefaciens can also be by nutrition and competition for space
Suppress the growth and breeding of phytopathogen.Research shows, bacillus amyloliquefaciens can effectively suppress aspergillus niger, Pyricularia oryzae,
The plurality of plant diseases such as Rhizoctonia solani Kuhn, bitter gourd wilt bacterium, Fusarium oxysporum, have broad application prospects.
Bacillus subtilis：Bacillus subtilis is a kind of shaft-like thin mesophilous aerobic sporiferous Gram-positive
Bacterium, can produce the endospore of heat-resisting, drought-enduring, uvioresistant and organic solvent.Bacillus subtilis pass through successfully to colonize to
Plant rhizosphere, body surface or internal, with the nutrition around pathogen competitive plant, secrete antibacterial material to suppress growth of pathogenic bacteria,
Plant defense system is induced to resist pathogen invasion simultaneously, so as to reach the purpose of biological and ecological methods to prevent plant disease, pests, and erosion.Bacillus subtilis can mainly press down
Make the plurality of plant diseases as caused by the phytopathogens such as filamentous fungi, including root disease, trunk disease, leaf, flower portion disease
Fruit disease after evil and results, is a kind of preferable Biocontrol microorganism.
Photosynthetic bacteria：The metabolite of photosynthetic bacteria is nutritious, rich in various B family vitamins, somatomedin, coenzyme
The physiological activator such as Q and antiviral agent, can activate the activity of vegetable plant cells, improve photosynthetic capacity.It is reported that photosynthetic
Bacterium has protective effect to the root of crop, and is conducive to beneficial microorganism to grow, and suppresses the growth of spoilage organisms, improves soil
Fertility, so as to improve vegetable yields and quality.Photosynthetic bacteria can also be using nitrate as electron acceptor, by nitrate reductase
With the effect of nitrite reductase, nitrate reduction is discharged into amino acid or nitrogen, so that the nitric acid in reducing vegetables
Salt content.Secondly, photosynthetic bacteria has cooperative effect with the nitrogenase activity of blown-ball Azotobacter.Photosynthetic bacteria promotes blown-ball Azotobacter
Generation can be secreted into extracellular metabolin, and this metabolin influences blown-ball Azotobacter in turn again, so that its nitrogenase activity
Improve.
Streptomyces microflavus：Streptomyces microflavus are a kind of typical actinomyces, can produce netropsin, spiramvcin, are suppressed
Gram-positive and negative bacteria, saccharomycete, the growth and breeding of filamentous fungi.Research shows that streptomyces microflavus are to clover downy mildew
The pathogen of crop such as disease, powdery mildew, brown spot, root rot show good prevention effect, and the upper diseases prevention that is often used in agriculture is protected
Seedling；Streptomyces microflavus also act as bacterial manure, convert Nitrogen In Soils P elements, increase soil fertility, and have stimulation plant growth
Effect.
A kind of preparation method of the liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, comprises the following steps：
(1) respectively by blown-ball Azotobacter, gel-shaped bacillus, phosphorus decomposing bacillus megaterium, bacillus amyloliquefaciens, withered
The parent species of careless bacillus, photosynthetic bacteria and streptomyces microflavus are inoculated in first cell culture medium and are cultivated, and obtain first class inoculum；
(2) above-mentioned first class inoculum is inoculated in into secondary medium respectively to be cultivated, obtains second class inoculum；
(3) above-mentioned second class inoculum is inoculated in into fermentation medium respectively to be cultivated, obtains zymocyte liquid；
(4) each zymocyte liquid is mixed in proportion and both obtains liquid microbe fertilizer.
Each function bacterium condition of culture of the present invention and method are following (each medium component is by percentage to the quality)：
Blown-ball Azotobacter
One-level culture
First cell culture medium：Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, chlorination
Sodium 0.02%, agar 3%, balance of water, pH 7.0-7.2.25-27 DEG C of cultivation temperature, incubation time 2-3 days.
Two grades of cultures
Secondary medium：Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, chlorination
Sodium 0.02%, balance of water, pH 7.0-7.2.25-27 DEG C of cultivation temperature, incubation time 3-5 days.
Fermented and cultured
Fermentation medium：Sodium acetate 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, carbonic acid
Sodium 0.05%, magnesium sulfate 0.05%, balance of water, pH 7.0-7.2.25-27 DEG C of cultivation temperature, incubation time 3-5 days.
Gel-shaped bacillus
One-level culture
First cell culture medium：Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, chlorination
Sodium 0.02%, yeast extract 0.05%, agar 3%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, incubation time 2-3 days.
Two grades of cultures
Secondary medium：Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, chlorination
Sodium 0.02%, yeast extract 0.05%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, incubation time 3-5 days.
Fermented and cultured
Fermentation medium：Sodium acetate 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, carbonic acid
Sodium 0.05%, magnesium sulfate 0.05%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, incubation time 5-7 days.
Phosphorus decomposing bacillus megaterium
One-level culture
First cell culture medium：Potato 20%, glucose 2%, agar 2%, balance of water, pH 7.0.Cultivation temperature 25-27
DEG C, incubation time 2-3 days.
Two grades of cultures
Secondary medium：Potato 20%, glucose 2%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, culture
3-5 days time.
Fermented and cultured
Fermentation medium：Sodium acetate 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, carbonic acid
Sodium 0.05%, magnesium sulfate 0.05%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, incubation time 5-7 days.
Bacillus amyloliquefaciens
One-level culture
First cell culture medium：Beef extract 0.3%, peptone 1%, sodium chloride 0.5%, agar 2%, balance of water, pH 7.0.
25-27 DEG C of cultivation temperature, incubation time 2-3 days.
Two grades of cultures
Secondary medium：Beef extract 0.3%, peptone 1%, sodium chloride 0.5%, balance of water, pH 7.0.Cultivation temperature
25-27 DEG C, incubation time 3-5 days.
Fermented and cultured
Fermentation medium：Sodium acetate 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, carbonic acid
Sodium 0.05%, magnesium sulfate 0.05%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, incubation time 5-7 days.
Bacillus subtilis
One-level culture
First cell culture medium：Beef extract 0.3%, peptone 1%, sodium chloride 0.5%, agar 2%, balance of water, pH 7.0.
25-27 DEG C of cultivation temperature, incubation time 2-3 days.
Two grades of cultures
Secondary medium：Beef extract 0.3%, peptone 1%, sodium chloride 0.5%, balance of water, pH 7.0.Cultivation temperature
25-27 DEG C, incubation time 3-5 days.
Fermented and cultured
Fermentation medium：Sodium acetate 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, carbonic acid
Sodium 0.05%, magnesium sulfate 0.05%, balance of water, pH 7.0.25-27 DEG C of cultivation temperature, incubation time 5-7 days.
Photosynthetic bacteria
One-level culture
First cell culture medium：Yeast extract 0.5%, peptone 0.5%, sodium carbonate 0.05%, sodium chloride 0.05%, phosphoric acid hydrogen two
Potassium 0.05%, magnesium sulfate 0.05%, balance of water, pH 6-10.Intensity of illumination 3000-4000 luxs, cultivation temperature 28-35
DEG C, incubation time 6-8 days.
Two grades of cultures
Secondary medium：Yeast extract 0.5%, peptone 0.5%, sodium carbonate 0.05%, sodium chloride 0.05%, phosphoric acid hydrogen two
Potassium 0.05%, magnesium sulfate 0.05%, ferric citrate 0.1%, balance of water, pH 6-10.Intensity of illumination 3000-4000 Le gram
This, 28-35 DEG C of cultivation temperature, incubation time 5-7 days.
Fermented and cultured
Fermentation medium：Sodium acetate 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, carbonic acid
Sodium 0.05%, magnesium sulfate 0.05%, balance of water, pH 7-8.
By two grades of culture strain inoculation seeding tanks, intensity of illumination 3000-4000 luxs, 26-35 DEG C of cultivation temperature, culture
6-8 days time；Fermentation tank culture is inoculated in afterwards 2-3 days, condition of culture is ibid.
Streptomyces microflavus
One-level culture
First cell culture medium：Yeast extract 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, sulfuric acid
Magnesium 0.05%, sodium carbonate 0.05%, agar 2%, balance of water, pH7.0-7.4.28-32 DEG C of cultivation temperature, incubation time 5-7
My god.
Two grades of cultures
Secondary medium：Yeast extract 0.5%, peptone 0.5%, sodium chloride 0.05%, dipotassium hydrogen phosphate 0.05%, sulfuric acid
Magnesium 0.05%, sodium carbonate 0.05%, ferric citrate 0.1%, balance of water, pH 7.0-7.4.28-32 DEG C of cultivation temperature, training
Support 5-7 days time.
Fermented and cultured
Fermentation medium：Glucose 1-3%, yeast juice 1-2%, potassium dihydrogen phosphate 0.01-0.05%, sodium chloride 0.02-
0.05%, ferrous sulfate 0.001-0.005%, balance of water, pH 7.0-7.4.28-32 DEG C of cultivation temperature, incubation time 5-7
My god.
Each non-specified particular of function bacterium culture of the present invention, is carried out with the means of prior art.
Beneficial effects of the present invention：
1st, the blown-ball Azotobacter that the present invention is selected can dramatically increase the soil organism and total nitrogen content by nitrogen fixation,
Improve soil physico-chemical property；Gel-shaped bacillus has utilization and capacity of decomposition to phosphorus potassium mineral, can discharge potassium, increases soil
The content of earth rapid available phosphorus, alkali-hydrolyzable nitrogen and available potassium；Phosphorus decomposing bacillus megaterium cannot directly utilize to plant in lecithin, soil
ADSORPTION STATE organophosphor, Phos have obvious decomposition；The activity of photosynthetic bacteria energy activated plant cell, improves plant light
Ability to function is closed, and root to crop has protective effect, four is mutually coordinated, collective effect, can significantly improve soil physical chemistry
Property, promotes the conversion of soil nutrient, increases soil fertility, and for plant provides nitrogen, phosphorus, potassium element, promotes plant growth, improves
Vegetable yields and quality, while overcome the excessive of chemical fertilizer applying and uneven the drawbacks of causing of applying fertilizer；
2nd, streptomyces microflavus, bacillus amyloliquefaciens and the bacillus subtilis that the present invention is selected can secrete antibacterial
Matter, effectively suppresses the growth and breeding of phytopathogen, to downy mildew of alfalfa, powdery mildew, brown spot, root rot, aspergillus niger, rice blast
The plurality of plant diseases such as disease, rice sheath blight disease, bitter gourd wilt have notable preventive effect, are biological preferable biological and ecological methods to prevent plant disease, pests, and erosions；
3rd, the present invention is from blown-ball Azotobacter, gel-shaped bacillus, phosphorus decomposing bacillus megaterium, solution starch gemma bar
Bacterium, bacillus subtilis, photosynthetic bacteria and streptomyces microflavus as microbial manure major function flora, products obtained therefrom is not only
With significant fixing nitrogen, dissolving phosphor and dissolving potassium and disease-resistant effect, soil bacterial community total metabolism activity can also be strengthened and community function is more
Sample, improves soil microenvironment, promotes soil beneficial microorganism growth and breeding, suppresses the growth of pathogen, is green
Agricultural, the preferable fertilizer of organic agriculture, there is bright prospects in agricultural sustainable development.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, the volume of each zymocyte liquid is based on parts by volume
For：10 parts of blown-ball Azotobacter, 3 parts of gel-shaped bacillus, 10 parts of phosphorus decomposing bacillus megaterium, 10 parts of bacillus amyloliquefaciens,
1 part of bacillus subtilis, 5 parts of photosynthetic bacteria, 1 part of streptomyces microflavus；
Embodiment 2
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, the volume of each zymocyte liquid is based on parts by volume
For：8 parts of blown-ball Azotobacter, 9 parts of gel-shaped bacillus, 8 parts of phosphorus decomposing bacillus megaterium, 8 parts of bacillus amyloliquefaciens, withered grass
3 parts of bacillus, 4 parts of photosynthetic bacteria, 3 parts of streptomyces microflavus；
Embodiment 3
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, the volume of each zymocyte liquid is based on parts by volume
For：It is 6 parts of blown-ball Azotobacter, 18 parts of gel-shaped bacillus, 6 parts of phosphorus decomposing bacillus megaterium, 6 parts of bacillus amyloliquefaciens, withered
6 parts of careless bacillus, 3 parts of photosynthetic bacteria, 6 parts of streptomyces microflavus；
Embodiment 4
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, the volume of each zymocyte liquid is based on parts by volume
For：It is 1 part of blown-ball Azotobacter, 30 parts of gel-shaped bacillus, 1 part of phosphorus decomposing bacillus megaterium, 1 part of bacillus amyloliquefaciens, withered
10 parts of careless bacillus, 0.5 part of photosynthetic bacteria, 10 parts of streptomyces microflavus；
Embodiment 5
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, the volume of each zymocyte liquid is based on parts by volume
For：It is 3 parts of blown-ball Azotobacter, 24 parts of gel-shaped bacillus, 3 parts of phosphorus decomposing bacillus megaterium, 3 parts of bacillus amyloliquefaciens, withered
8 parts of careless bacillus, 1.5 parts of photosynthetic bacteria, 8 parts of streptomyces microflavus；
Above-described embodiment 1~5, a kind of preparation method of the liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, bag
Include following steps：
(1) respectively by blown-ball Azotobacter, gel-shaped bacillus, phosphorus decomposing bacillus megaterium, bacillus amyloliquefaciens, withered
The parent species of careless bacillus, photosynthetic bacteria and streptomyces microflavus are inoculated in first cell culture medium and are cultivated, and obtain first class inoculum；
(2) above-mentioned first class inoculum is inoculated in into secondary medium respectively to be cultivated, obtains second class inoculum；
(3) above-mentioned second class inoculum is inoculated in into fermentation medium respectively to be cultivated, obtains zymocyte liquid；
(4) each zymocyte liquid is mixed in proportion and both obtains liquid microbe fertilizer.
Experimental example 1
A kind of measure of liquid microbe fertilizer technology index with fixing nitrogen, dissolving phosphor and dissolving potassium effect of the invention
1st, test objective：Detect in a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect of the invention effectively
Viable count, miscellaneous bacteria rate, pH.
2nd, sample is detected：By a kind of liquid microbe with fixing nitrogen, dissolving phosphor and dissolving potassium effect prepared by above-described embodiment 1~5
Fertilizer sample.
3rd, detection method：National standard (GB20287-2006) according to microbial manure is detected.
4th, testing result：Testing result is as shown in table 1 below.
The technical indicator measurement result of table 1
5th, conclusion
After testing, a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect of the invention meets microbial manure
Living bacteria count highest contained in national standard, and embodiment 3, is 4.3 hundred million/milliliter, and miscellaneous bacteria rate is minimum, is 3.7%.
Experimental example 2
A kind of suppression of the liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect of the invention to pathogen of crop
Effect
1st, test objective：By in vitro indoors, method is suppressed using mycelial growth, detect that the product is supplied 5 kinds
Try the inhibitory action of pathogen of crop.
2nd, sample is detected：By a kind of liquid microbe with fixing nitrogen, dissolving phosphor and dissolving potassium effect prepared by above-described embodiment 1~5
Fertilizer sample.
3rd, assay method and step：
Method detection is suppressed to 5 kinds of inhibitory action for examination pathogen of crop using mycelial growth indoors, sample is dilute
It is interpreted into 30,60,90,120 times, each plate and adds 1mL dilute samples and 9mL potato culture mediums to shake up, then cultured
The pathogen card punch of diameter 5mm breaks into bacterium colony block and is put into above-mentioned plate center, with plus with sample equivalent sterilized water to compare,
Each concentration is repeated 3 times, and is put into 26-28 DEG C of constant incubator culture 66-70h, calculates mycelial growth inhibition rate.
4th, measurement result：Testing result is as shown in table 2 below.
2 pairs of inhibitory action for examination pathogen of crop of table
5th, conclusion
After testing, 5 kinds of inhibiting rate highests for examination pathogen of crop of embodiment 3 pair, show that embodiment 3 is the present invention one
Plant the optimum formula of the liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect.In embodiment 3, when Sample Dilution 30,60,
At 100 times, the inhibiting rate to botrytis cinerea is 100%；When 30,60 times of Sample Dilution, to water spinach pine root fungus, strawberry
The inhibiting rate of Powdery Mildew is 100%；When 30 times of Sample Dilution, the inhibiting rate to rice blast fungus is 100%, to cucumber
The inhibiting rate of wilt is 77.8%.
Experimental example 3
A kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect of the invention is to tomato economical character and yield
Influence
1st, experimental design
For studying thing for tomato, kind is " Di Rui ", and planting density is 3000 plants/acre；Set two treatment altogether, 3 repetitions,
Plot area is 20m2, random district's groups arrangement.Each treatment sets as follows：
Treatment A：Habits fertilization, composite fertilizer (N-P2O5-K2O=15-15-15) 50kg/ mus；
Treatment B：Liquid fertilizer 3kg/ mus prepared by above-described embodiment 3 carries out pouring root, composite fertilizer (N-P2O5-K2O=15-
15-15) 50kg/ mus.
2nd, fertilization time and method
When site preparation applies fertilizer to the subsoil, applied by every mu of 50kg composite fertilizer and supplied in examination soil, then ridging.Choose growing way uniform
After the healthy seedling unification field planting of cause, field planting 2 weeks, treatment B is filled that to apply the liquid with fixing nitrogen, dissolving phosphor and dissolving potassium effect micro- by every mu of 3kg
Bio-feritlizer.Experimental plot others base manure, topdress, irrigate and the condition such as other field management is consistent.
3rd, pilot survey
Receipts phase determine that its plant height, stem be thick, individual plant is beared fruit number, single fruit weight and yield is contained in tomato, sample collection is every cell
20~30 plants of investigation, averages.
4th, result of the test：Result is as shown in table 3 below.
Influence of the table 3 to tomato economical character and yield
5th, conclusion
By the investigation statisticses to tomato Other Main Agronomic Characters and yield, using of the invention a kind of with fixed nitrogen solution
The liquid microbe fertilizer of phosphorus potassium decomposing effect significantly improves the economical character of tomato, and yield is dramatically increased.With habits fertilization phase
Than, treatment B plant heights, stem are thick, individual plant is beared fruit number, single fruit weight and yield increase by 15.8% respectively, 11.6%, 28%, 4.9%,
10.89%, illustrate contained beneficial micro- life in a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect of the invention
Thing can more comprehensively provide tomato growth needed for nutrient, promote tomato growth, be obviously improved tomato economical character, produce
Amount is dramatically increased.
Claims (6)
1. a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect, it is characterised in that by following microbial fermentation solution
Parts by volume composition：1~10 part of blown-ball Azotobacter, 3~30 parts of gel-shaped bacillus, 1~10 part of phosphorus decomposing bacillus megaterium,
1~10 part of bacillus amyloliquefaciens, 1~10 part of bacillus subtilis, 0.5~5 part of photosynthetic bacteria, 1~10 part of streptomyces microflavus.
2. a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect according to claim 1, it is characterised in that
It is made up of the parts by volume of following microbial fermentation solution：3~8 parts of blown-ball Azotobacter, 9~24 parts of gel-shaped bacillus, phosphorus decomposing are huge
It is 3~8 parts of Bacterium anthracoides, 3~8 parts of bacillus amyloliquefaciens, 3~8 parts of bacillus subtilis, 1.5~4 parts of photosynthetic bacteria, thin
3~8 parts of yellow streptomycete.
3. a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect according to claim 2, it is characterised in that
It is made up of the parts by volume of following microbial fermentation solution：6 parts of blown-ball Azotobacter, 18 parts of gel-shaped bacillus, the huge gemma of phosphorus decomposing
6 parts of bacillus, 6 parts of bacillus amyloliquefaciens, 6 parts of bacillus subtilis, 3 parts of photosynthetic bacteria, 6 parts of streptomyces microflavus.
4. a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect according to claim 3, it is characterised in that
The photosynthetic bacteria be selected from Rhodopseudomonas palustris, plus black red pseudomonas or Rhodopseudomonas spheroides in one or more.
5. a kind of a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect as described in any one of Claims 1 to 4
Preparation method, comprise the following steps：
(1) respectively by blown-ball Azotobacter, gel-shaped bacillus, phosphorus decomposing bacillus megaterium, bacillus amyloliquefaciens, withered grass bud
The parent species of spore bacillus, photosynthetic bacteria and streptomyces microflavus are inoculated in first cell culture medium and are cultivated, and obtain first class inoculum；
(2) above-mentioned first class inoculum is inoculated in into secondary medium respectively to be cultivated, obtains second class inoculum；
(3) above-mentioned second class inoculum is inoculated in into fermentation medium respectively to be cultivated, obtains zymocyte liquid；
(4) each zymocyte liquid is mixed in proportion and both obtains liquid microbe fertilizer.
6. a kind of liquid microbe fertilizer with fixing nitrogen, dissolving phosphor and dissolving potassium effect as described in any one of Claims 1 to 5 is in water
Application in rice, vegetables, melon and fruit, tobacco, cotton, flowers, Chinese herbal medicine plantation.
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Application publication date: 20170609 |