CN107144554A - A kind of aflatoxin detection means - Google Patents
A kind of aflatoxin detection means Download PDFInfo
- Publication number
- CN107144554A CN107144554A CN201710456830.9A CN201710456830A CN107144554A CN 107144554 A CN107144554 A CN 107144554A CN 201710456830 A CN201710456830 A CN 201710456830A CN 107144554 A CN107144554 A CN 107144554A
- Authority
- CN
- China
- Prior art keywords
- reflecting
- aflatoxin
- detection means
- grating
- illuminator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of aflatoxin detection means, the aflatoxin detection means includes：Materiel delivery system, illuminator, illuminator is arranged on the side of materiel delivery system, materiel delivery system transports testing sample after the illumination region of illuminator, aflatoxin in the ultraviolet excitation testing sample that illuminator is sent produces fluorescence signal, and reflecting system, reflecting grating, reflecting system, reflecting grating and illuminator are arranged on the same side of materiel delivery system, reflecting system reflexes to fluorescence signal in reflecting grating, fluorescence signal is cut into multiple light beams by reflecting grating, and forms interference pattern according to multiple light beams.The present invention is by using reflecting system and reflecting grating, so as to be laid out by the reflective structure, it is to avoid the problem of transmission-type structure absorbs to week fluorescent, and improves week fluorescent interference pattern picture contrast, and then improves accuracy of detection.
Description
Technical field
The present invention relates to technical field of optical detection, it particularly relates to a kind of aflatoxin detection means.
Background technology
Aflatoxin (Aflatoxin, abbreviation AF) is to produce a kind of fungi toxic metabolite by aspergillus flavus and aspergillus parasiticus
Product, is also the toxicity found so far a most strong class mycotoxin.Aflatoxin mainly have AFB1 (aflatoxin B1),
This 5 class of AFB2, AFG1, AFG2, AFM1.Wherein, AFB1 toxicity is most strong, and its toxicity is 10 times of potassium cyanide, is the 68 of arsenic
Times, it is 416 times of melamine.Moreover, AFB1 carciongenic potencies are 75 times of dimethyl nitramine, international tumor research machine in 1993
Structure (International Agency for Research on Cancer, IARC) has just been classified as I class carcinogenic substances, its
Its 4 class is listed in II class carcinogenic substances.Not only toxicity is big for aflatoxin, content is low, and pollution range is wide, in grain, oil, tea, dry
Really, find there is aflatoxin contamination in more than the 100 kinds of agricultural product such as breast, feed, soy sauce and food.Therefore, aspergillus flavus is carried out
Toxin detection technology research is particularly significant.
The detection technique on aflatoxin mainly includes damage detection and the class of Non-Destructive Testing two at present.Damage detection skill
Art is predominantly based on materialization principle (such as：High performance liquid chromatography, liquid chromatography-mass spectrography/mass spectrography etc.), it has to sample
Destructiveness, carries out purification pretreatment etc., detection time is long, is not suitable for the industrial on-line checking of high-volume.And non-destructive testing technology
With to detection the nondestructive advantage of object, as people study focus.The non-destructive testing technology reported at present is typically
Using the detection technique of Ultraluminescence intensity image, it mainly irradiates object to be measured using long wave ultraviolet and excites visible fluorescence, and ties
Close camera and obtain visible fluorescence intensity image.There is publication number currently with the progress aflatoxin detection of this technology
CN105044062A patent discloses a kind of aflatoxin on-line measuring device and the material separation device using the device)
A kind of aflatoxin detection and sorting unit etc. are disclosed with publication number CN103234945A patent.Because this technology is direct
Obtain be visible fluorescence picture, but generally burst of ultraviolel produce fluorescence it is very weak, cause intensity image resolution ratio it is relatively low and
Noise is poor, and it can influence the accuracy of detection.
In addition, interference phase measurement technology is the interference pattern that intensity coherent superposition is formed using optical interference, and then from dry
Relate to and the related phase mehtod of object physical quantity to be measured is obtained in figure.Compared with aforementioned optical imaging technique, interfere phase measurement skill
Art has wide range, high sensitivity and high-precision feature, is used widely in precision optics detection field.2009,
Pierre Bon1 et al. deliver entitled " the Quadriwave lateral shearing of opinion in Optics Express
In interferometry for quantitative phase microscopy of living cells " papers, report
The phase image of living body biological under dim light is obtained using two-dimensional transmission grating shearing interferometer.It is relative with directly obtaining figure
Picture, phase image further increases picture contrast.However, reporting that detection means light path integrally uses transmission-type in this text
Layout structure, it is reducing light wave transmissions intensity in transmission to a certain degree, is having influence on interference pattern intensity distribution, limit a phase
The further raising of measurement accuracy.
The problem of in correlation technique, effective solution is not yet proposed at present.
The content of the invention
The problem of in correlation technique, the present invention proposes a kind of aflatoxin detection means, so as to overcome existing purple
The deficiency of outer fluorescence imaging, the detection means uses reflective structure, it is to avoid the problem of transmission-type structure absorbs to week fluorescent,
Improve the efficiency of light energy utilization.In addition, being designed using reflective two-dimentional phase grating, ± 3 grades and ± 3 grades of times of order diffraction light can be eliminated
Influence so that the interference light wave formed is concentrated mainly in ± 3 order diffraction light, so as to improve accuracy of detection.
The technical proposal of the invention is realized in this way：
According to an aspect of the invention, there is provided a kind of aflatoxin detection means.
The aflatoxin detection means includes：Materiel delivery system, illuminator, illuminator are arranged on material transmission
The side of system, materiel delivery system transports testing sample after the illumination region of illuminator, and it is ultraviolet that illuminator is sent
Light excites the aflatoxin in testing sample to produce fluorescence signal, and reflecting system, reflecting grating, reflecting system, reflection
Grating and illuminator are arranged on the same side of materiel delivery system, and reflecting system reflexes to fluorescence signal in reflecting grating,
Fluorescence signal is cut into multiple light beams by reflecting grating, and forms interference pattern according to multiple light beams.
According to one embodiment of present invention, reflecting grating includes：Substrate；And on the vertical and horizontal of substrate array
The reflecting part and phase delay reflecting part of arrangement, reflecting part and phase delay reflecting part are adjacent in longitudinally and laterally away
Set, non-reflective alternatively non-transparent part is provided between adjacent reflecting part and phase delay reflecting part, wherein, reflecting part
It is arranged on non-reflective alternatively non-transparent part above substrate, phase delay part is arranged in the groove of substrate.
According to one embodiment of present invention, reflecting part and phase delay reflecting part are square, reflecting part
It is identical with the size that phase delay reflects.
According to one embodiment of present invention, the first square length of side and second horizontally-parallel, the square length of side with
Parallel longitudinal, first length of side and second length of side are vertical.
According to one embodiment of present invention, the first square length of side is with being in laterally 45 ° of angles, the second square side
Long is in 45 ° with longitudinal direction, and first length of side and second length of side are vertical.
According to one embodiment of present invention, the material of substrate is SiO2, the material of emitting portion is Al, and phase delay is anti-
The material for penetrating part is Al, and the material of non-reflective alternatively non-transparent part is Cr.
According to one embodiment of present invention, fluorescence signal is divided into four light beams, four beam interference shapes by reflecting grating
Into two-dimentional interference pattern.
According to one embodiment of present invention, irradiation system includes：First lighting unit, the second lighting unit, first shines
The illumination region of bright unit and the illumination region of the second lighting unit are overlapped.
According to one embodiment of present invention, the front end of the first lighting unit and the second lighting unit is provided with Fresnel
Lens or post lens.
According to one embodiment of present invention, aflatoxin detection means also includes：Photoelectric detection unit, signal transacting
Unit, control unit, photodetector unit and signal processing unit connection, signal processing unit and control unit connection, photoelectricity
Two-dimentional interference pattern is transferred to signal processing unit by probe unit, and signal processing unit is handled two-dimentional interference pattern, with true
Determine whether contain aflatoxin in testing sample, control unit control rejecting mechanism is sorted to testing sample.
The advantageous effects of the present invention are：
1) aflatoxin detection means of the invention employs reflecting system and reflecting grating, so that reflective by this
Topology layout, it is to avoid the problem of transmission-type structure absorbs to week fluorescent, and raising week fluorescent interference pattern picture contrast,
And then improve accuracy of detection；
2) phase image of testing sample is obtained by interference imaging, relative to existing Ultraluminescence imaging technique, sheet
The phase image that invention is obtained can further improve picture contrast, improve detection accuracy；
3) the reflective two-dimentional phase grating that the present invention is designed using special construction only produces four beam reflected lights, reduce or
Other level diffraction light influences are eliminated, so as to improve the degree of accuracy of detection.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing needed to use is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, on the premise of not paying creative work, can also be obtained according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is the schematic diagram of aflatoxin detection means according to embodiments of the present invention；
Fig. 2 is the top view of the first reflecting grating according to embodiments of the present invention；
Fig. 3 is the sectional view of the first reflecting grating according to embodiments of the present invention；
Fig. 4 is the top view of the second reflecting grating according to embodiments of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belongs to what the present invention was protected
Scope.
There is provided a kind of aflatoxin detection means for embodiments in accordance with the present invention.
As shown in figure 1, aflatoxin detection means according to embodiments of the present invention includes：Materiel delivery system 101, photograph
Bright system 201, illuminator 201 is arranged on the side of materiel delivery system 101, and materiel delivery system 101 is by testing sample 102
After the illumination region for transporting illuminator 201, the aspergillus flavus in the ultraviolet excitation testing sample 102 that illuminator 201 is sent
Toxin produces fluorescence signal, and reflecting system 202, reflecting grating 203, reflecting system 202, reflecting grating 203 and illumination system
System 201 is arranged on the same side of materiel delivery system 101, and reflecting system 202 reflexes to fluorescence signal in reflecting grating 203,
Fluorescence signal is cut into multiple light beams by reflecting grating 203, and forms interference pattern according to multiple light beams.
By means of the above-mentioned technical proposal of the present invention, by using reflecting system and reflecting grating, so that anti-by this
Penetrate formula topology layout, it is to avoid the problem of transmission-type structure absorbs to week fluorescent, and improve week fluorescent interference pattern image pair
Than degree, and then improve accuracy of detection.
According to one embodiment of present invention, reflecting grating 203 includes：Substrate；And on the vertical and horizontal of substrate
The reflecting part b1 and phase delay reflecting part b2, reflecting part b1 and phase delay reflecting part b2 of array arrangement are along vertical
Set to laterally adjacent, non-reflective alternatively non-transparent portion is provided between adjacent reflecting part b1 and phase delay reflecting part b2
Divide a1, wherein, reflecting part b1 and non-reflective alternatively non-transparent part a1 are arranged on above substrate, and phase delay part is arranged on substrate
Groove in.
According to one embodiment of present invention, reflecting part b1 and phase delay reflecting part b2 are square, reflection
Part b1 is identical with the size that phase delay reflects.
According to one embodiment of present invention, the first square length of side and second horizontally-parallel, the square length of side with
Parallel longitudinal, first length of side and second length of side are vertical.
According to one embodiment of present invention, the first square length of side is with being in laterally 45 ° of angles, the second square side
Long is in 45 ° with longitudinal direction, and first length of side and second length of side are vertical.
According to one embodiment of present invention, the material of substrate is SiO2, the material of emitting portion is Al, and phase delay is anti-
The material for penetrating part b2 is Al, and non-reflective alternatively non-transparent part a1 material is Cr.
According to one embodiment of present invention, fluorescence signal is divided into four light beams, four beam interferences by reflecting grating 203
Form two-dimentional interference pattern.
According to one embodiment of present invention, irradiation system includes：First lighting unit, the second lighting unit, first shines
The illumination region of bright unit and the illumination region of the second lighting unit are overlapped.
According to one embodiment of present invention, the front end of the first lighting unit and the second lighting unit is provided with Fresnel
Lens or post lens.
According to one embodiment of present invention, aflatoxin detection means also includes：Photoelectric detection unit 204, signal
Processing unit 205, control unit 206, photodetector unit and signal processing unit 205 are connected, signal processing unit 205 and control
Unit 206 processed is connected, and two-dimentional interference pattern is transferred to signal processing unit 205, signal processing unit by photoelectric detection unit 204
205 pairs of two-dimentional interference patterns are handled, and to determine whether to contain in testing sample 102 aflatoxin, control unit 206 is controlled
Rejecting mechanism is sorted to testing sample 102.
Above-mentioned technical proposal is described in detail below by specific embodiment in order to preferably describe the present invention.
As shown in figure 1, the aflatoxin detection means includes materiel delivery system 101, testing sample 102, illumination system
System 201, reflecting system (or reflective colimated light system) 202, reflecting grating (or reflective two-dimentional phase grating) 203, photoelectricity are visited
Unit 204, signal processing unit 205 and control unit 206 are surveyed, wherein, the reflecting system 202, reflecting grating 203 and illumination system
System 201 is arranged on the same side of materiel delivery system 101, and reflecting system 202 is in arc-shaped, the tangent line and thing at the midpoint of the circular arc
Expect conveyer belt in transmission system 101 at an angle, while it is arranged on the top of illuminator 201, reflecting grating 203 with
Conveyer belt in materiel delivery system 101 is in 45 ° of settings.In addition, the energy in order to improve illumination region, the present invention, which will be illuminated, is
201 points of system is ultra violet illumination unit 201A and ultra violet illumination unit 201B, and both of which is laid out using ultraviolet LED one-dimensional array, and
And first purple that sends of lighting unit (or ultra violet illumination unit 201A) and the second lighting unit (or ultra violet illumination unit 201B)
Outer Line beam is set with the conveyer belt in materiel delivery system 101 in 45 °, and ultra violet illumination unit 201A illumination region
Overlapped with ultra violet illumination unit 201B illumination region.While the uniformity in order to improve illumination region, ultra violet illumination unit
Fresnel Lenses or post lens are provided with before 201A and ultra violet illumination unit 201B is used for even light.
In addition, the specific detection process of the aflatoxin detection means is：
After materiel delivery system 101 transports Uniform Illumination region of the testing sample 102 by illuminator 201, illumination system
Aflatoxin in the ultraviolet excitation testing sample 102 that system 201 is sent produces fluorescence signal (or visible fluorescence).Treat test sample
Product 102 are irradiated the reflecting system 202 that the visible fluorescence excited enters in detection transducing part, the reflection system by illuminator 201
System 202 is by incident fluorescence collimated incident to reflecting grating 203；Reflecting grating 203 is designed using special construction, is primarily served point
The effect of light shearing elements, (+1 ,+1), (- 1 ,+1), (- 1, -1), (+1, -1) four beam are cut into by incident visible fluorescence light splitting
Reflected light, above-mentioned four beams reflecting interference forms two-dimentional interference pattern, and reception is detected by photoelectric detection unit 204, wherein, the photoelectricity
Probe unit 204 can be area array CCD detector.The interference pattern of detection is transferred to signal processing unit by photoelectric detection unit 204
205 are handled, and obtain correspondence aflatoxin material level phasor, and with being contrasted without infection aflatoxin material level phasor.
Threshold value with setting is worth according to contrast difference, point of rejecting mechanism progress aflatoxin material is controlled using control unit 205
Choosing.
Reflecting grating 203 includes two kinds of structures of reflecting grating I or reflecting grating II, specifically：With continued reference to Fig. 2 and 3,
The reflecting part b1 and phase delay reflecting part b2 of array arrangement, reflecting part on the two-dimensional directional (vertical and horizontal) of substrate
B1 and phase delay reflecting part b2 are disposed adjacent in longitudinally and laterally away, adjacent reflecting part b1 and phase delay reflecting part
Non-reflective alternatively non-transparent part a1, i.e. reflecting part b1 are provided between point b2 and non-reflective alternatively non-transparent part a1 is arranged in substrate
Side, phase delay part is arranged on the cycle phases of the groove internal reflection grating I of substrate in the two-dimensional direction together, and both direction
Comprising non-reflective alternatively non-transparent unit and reflection light unit in a cycle.Wherein, non-reflective alternatively non-transparent unit is comprising non-reflective
Alternatively non-transparent part a1 and non-reflective alternatively non-transparent part a2, reflected beam portion includes direct reflecting part b1 and phase delay reflecting part
Divide b2, moreover, a1 is identical with a2 size, b1 is identical with b2 size, and reflecting part b1 and phase delay reflecting part b2 are equal
Square, reflecting part b1 is identical with the size that phase delay reflects, at the same first length of side of the square with it is horizontally-parallel,
Square second length of side with it is parallel longitudinal, first length of side and second length of side are vertical.Similarly, the reflecting grating II in Fig. 4 with it is anti-
Penetrate that grating I is similar, its be specifically distinguished as first length of side of square with laterally in 45 ° of angles, square second length of side with it is vertical
To in 45 °, first length of side and second length of side are vertical.Reflective two-dimentional phase grating 203 is designed using above-mentioned special construction, will be entered
Irradiating light beam is diffracted primarily into (+1 ,+1), (- 1 ,+1), (- 1, -1), (+1, -1) four beam reflected light, and other levels are reduced or eliminated
Diffraction light influences.
In addition, the base material of reflecting grating 203 uses SiO2, non-reflective alternatively non-transparent part is Cr films, reflecting grating 203
In directly reflecting part b1 be Al films, and phase delay reflecting part b2 is that the substrate of reflecting grating 203 is etched and obtained, after etching
Surface is coated with Al films.
Illustrated below by taking reflecting grating I as an example, if the alternatively non-transparent part a1 and alternatively non-transparent part a2 of reflecting grating 203
Width be Wa, directly reflecting part b1 and phase delay reflecting part b2 width is W in reflected beam portionb, phase prolongs
Reflecting part b2 is relative to direct reflecting part b1 bit phase delay late.Theoretical, the reflecting grating 203 according to Fourier transformation
Reflected light far-field intensity distribution can be expressed as：
Wherein, m, n are reflected light level.
It can be seen that from above-mentioned formula (1)：When direct reflecting part b1 and phase in reflecting grating I reflected beam portion
Delayed reflex part b2 phase differencesWhen, now reflecting grating I reflection 0 grade and all even number level diffraction lights disappear
Lose；Work as Wa=2WbWhen, ± 3 grades of reflecting grating I and ± 3 times order diffraction light disappear, and reflecting grating I is by incident light wave master
It is diffracted into (+1 ,+1), (- 1 ,+1), (- 1, -1), (+1, -1) four beam reflected light.
Similarly, based on similar foundation, reflecting grating II also incident light wave can be mainly diffracted into (+1 ,+1), (- 1 ,+
1), (- 1, -1), (+1, -1) four beam reflected light.
In addition, phase delay reflecting part b2 etching depths are calculated as follows：
According to Fig. 4, it is assumed that phase delay reflecting part b2 etching depths are that h introducings position is mutually in reflecting grating 203By
In the etched transmission mediums of phase delay reflected beam portion b2 be air, then according to the relation between optical path difference and position phase, relatively
In direct reflected beam portion b1, the bit phase delay calculation formula that phase delay reflected beam portion b2 is introduced is as follows：
Wherein, in order to reduce reflection visible fluorescence in spectrum influence, negate the mean wavelength for penetrating visible fluorescenceCarry out
Calculate.
Understood according to above analysis, obtain phase delay reflecting part b2 etching depths so as to be calculated with reference to formula (2) and be：
Therefore, reflecting grating 203 of the invention by incident fluorescence signal be mainly diffracted into (+1 ,+1), (- 1 ,+1), (-
1, -1), (+1, -1) four beam reflected light.
In summary, by means of the above-mentioned technical proposal of the present invention, by using reflecting system and reflecting grating, so that
It is laid out by the reflective structure, it is to avoid the problem of transmission-type structure absorbs to week fluorescent, and improves week fluorescent interference
Figure picture contrast, and then improve accuracy of detection；The phase image of testing sample is obtained by interference imaging, relative to existing
Ultraluminescence imaging technique, the phase image that the present invention is obtained can further improve picture contrast, improve detection accuracy
The reflective two-dimentional phase grating that the present invention is designed using special construction only produces four beam reflected lights, and other levels are reduced or eliminated
Secondary diffraction light influence, so as to improve the degree of accuracy of detection.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (10)
1. a kind of aflatoxin detection means, including：Materiel delivery system, illuminator, the illuminator are arranged on institute
The side of materiel delivery system is stated, the materiel delivery system transports testing sample after the illumination region of the illuminator,
Aflatoxin in testing sample described in the ultraviolet excitation that the illuminator is sent produces fluorescence signal, and its feature exists
In, in addition to：Reflecting system, reflecting grating, the reflecting system, the reflecting grating and the illuminator are arranged on described
The same side of materiel delivery system, the reflecting system reflexes to the fluorescence signal in the reflecting grating, the reflection
The fluorescence signal is cut into multiple light beams by grating, and forms interference pattern according to the multiple light beam.
2. aflatoxin detection means according to claim 1, it is characterised in that the reflecting grating includes：Substrate；
And
The reflecting part of array arrangement and phase delay reflecting part, the reflecting part on the vertical and horizontal of the substrate
It is disposed adjacent with the phase delay reflecting part along the vertical and horizontal, adjacent reflecting part and phase delay reflection
Non-reflective alternatively non-transparent part is provided between part, wherein, the reflecting part and the non-reflective alternatively non-transparent part are arranged on
Above the substrate, the phase delay part is arranged in the groove of the substrate.
3. aflatoxin detection means according to claim 2, it is characterised in that the reflecting part and the phase
Delayed reflex part is square, and the reflecting part is identical with the size that the phase delay reflects.
4. aflatoxin detection means according to claim 3, it is characterised in that the first square length of side with
It is described horizontally-parallel, the second square length of side with it is described parallel longitudinal, first length of side and second length of side are hung down
Directly.
5. aflatoxin detection means according to claim 3, it is characterised in that the first square length of side with
The transverse direction is in 45 °, first length of side and described second with the longitudinal direction in 45 ° of angles, the second square length of side
The length of side is vertical.
6. aflatoxin detection means according to claim 2, it is characterised in that the material of the substrate is SiO2, institute
The material for stating emitting portion is Al, and the material of the phase delay reflecting part is Al, the material of the non-reflective alternatively non-transparent part
Expect for Cr.
7. aflatoxin detection means according to claim 2, it is characterised in that the reflecting grating is by the fluorescence
Signal is divided into four light beams, and four beam interferences form two-dimentional interference pattern.
8. aflatoxin detection means according to claim 1, it is characterised in that the irradiation system includes：First
The illumination region of lighting unit, the second lighting unit, the illumination region of first lighting unit and second lighting unit
Overlap.
9. aflatoxin detection means according to claim 8, it is characterised in that first lighting unit and second
The front end of lighting unit is provided with Fresnel Lenses or post lens.
10. aflatoxin detection means according to claim 7, it is characterised in that the aflatoxin detection dress
Putting also includes：Photoelectric detection unit, signal processing unit, control unit, the photodetector unit and the signal transacting list
Member connection, the signal processing unit and described control unit connection, the photoelectric detection unit pass the two-dimentional interference pattern
The signal processing unit is defeated by, the signal processing unit is handled the two-dimentional interference pattern, described to be measured to determine
Whether aflatoxin is contained in sample, and described control unit control rejecting mechanism is sorted to testing sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710456830.9A CN107144554A (en) | 2017-06-16 | 2017-06-16 | A kind of aflatoxin detection means |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710456830.9A CN107144554A (en) | 2017-06-16 | 2017-06-16 | A kind of aflatoxin detection means |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107144554A true CN107144554A (en) | 2017-09-08 |
Family
ID=59781594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710456830.9A Pending CN107144554A (en) | 2017-06-16 | 2017-06-16 | A kind of aflatoxin detection means |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107144554A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202101939U (en) * | 2011-05-17 | 2012-01-04 | 中国农业科学院油料作物研究所 | Measuring device for aflatoxin |
| US20120061586A1 (en) * | 2010-09-10 | 2012-03-15 | Haibo Yao | Method and detection system for detection of aflatoxin in corn with fluorescence spectra |
| CN102681365A (en) * | 2012-05-18 | 2012-09-19 | 中国科学院光电技术研究所 | Projection objective lens wave aberration detection device and method |
| CN103234945A (en) * | 2013-03-29 | 2013-08-07 | 合肥美亚光电技术股份有限公司 | Aflatoxin detecting and sorting apparatus |
| CN103246077A (en) * | 2013-05-10 | 2013-08-14 | 中国科学院上海光学精密机械研究所 | Device utilizing grating to realize object imaging |
| CN105044062A (en) * | 2015-07-31 | 2015-11-11 | 合肥美亚光电技术股份有限公司 | Online aflatoxin detecting device and material sorting equipment adopting same |
| CN204945039U (en) * | 2015-07-20 | 2016-01-06 | 中国水稻研究所 | Aflatoxin ultraviolet fluorescent detecting device |
| CN106442458A (en) * | 2016-11-29 | 2017-02-22 | 合肥泰禾光电科技股份有限公司 | Device for detecting aflatoxin by aid of ultraviolet fluorescence spectra in online manner |
| CN207248746U (en) * | 2017-06-16 | 2018-04-17 | 合肥泰禾光电科技股份有限公司 | A kind of aflatoxin detection device |
-
2017
- 2017-06-16 CN CN201710456830.9A patent/CN107144554A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120061586A1 (en) * | 2010-09-10 | 2012-03-15 | Haibo Yao | Method and detection system for detection of aflatoxin in corn with fluorescence spectra |
| CN202101939U (en) * | 2011-05-17 | 2012-01-04 | 中国农业科学院油料作物研究所 | Measuring device for aflatoxin |
| CN102681365A (en) * | 2012-05-18 | 2012-09-19 | 中国科学院光电技术研究所 | Projection objective lens wave aberration detection device and method |
| CN103234945A (en) * | 2013-03-29 | 2013-08-07 | 合肥美亚光电技术股份有限公司 | Aflatoxin detecting and sorting apparatus |
| CN103246077A (en) * | 2013-05-10 | 2013-08-14 | 中国科学院上海光学精密机械研究所 | Device utilizing grating to realize object imaging |
| CN204945039U (en) * | 2015-07-20 | 2016-01-06 | 中国水稻研究所 | Aflatoxin ultraviolet fluorescent detecting device |
| CN105044062A (en) * | 2015-07-31 | 2015-11-11 | 合肥美亚光电技术股份有限公司 | Online aflatoxin detecting device and material sorting equipment adopting same |
| CN106442458A (en) * | 2016-11-29 | 2017-02-22 | 合肥泰禾光电科技股份有限公司 | Device for detecting aflatoxin by aid of ultraviolet fluorescence spectra in online manner |
| CN207248746U (en) * | 2017-06-16 | 2018-04-17 | 合肥泰禾光电科技股份有限公司 | A kind of aflatoxin detection device |
Non-Patent Citations (6)
| Title |
|---|
| WANG HAI等: "Approach to characterize manufacture tolerances of two-dimensional cross-phase grating", 《OPTICAL ENGINEERING》 * |
| WANG HAI等: "Approach to characterize manufacture tolerances of two-dimensional cross-phase grating", 《OPTICAL ENGINEERING》, vol. 52, no. 10, 3 October 2013 (2013-10-03), pages 1 - 2, XP060025597, DOI: 10.1117/1.OE.52.10.104101 * |
| 刘志祥等: "大数值孔径物镜的波像差测量及其特殊问题", 《光学精密工程》 * |
| 刘志祥等: "大数值孔径物镜的波像差测量及其特殊问题", 《光学精密工程》, vol. 24, no. 3, 31 March 2016 (2016-03-31), pages 484 * |
| 王叶群等: "用于污染黄曲霉毒素花生分选的荧光信号研究", 《农业工程学报》 * |
| 王叶群等: "用于污染黄曲霉毒素花生分选的荧光信号研究", 《农业工程学报》, vol. 32, no. 01, 8 January 2016 (2016-01-08), pages 187 - 192 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI310653B (en) | Spatial information detecting device | |
| CN106442458A (en) | Device for detecting aflatoxin by aid of ultraviolet fluorescence spectra in online manner | |
| JP2013507608A (en) | Measuring instrument and method for determining properties of articles and their surfaces | |
| FI125408B (en) | Method and gauge for measuring surface distance, target thickness and optical properties | |
| CN102253020B (en) | Cavity enhanced detection apparatus for heavy metal content in air | |
| CZ200519A3 (en) | Spectroscopy of surface plasmons for sensors with surface plasmons and element for making the same | |
| US20010015414A1 (en) | Method and arrangement for inspection of surfaces | |
| CN207248746U (en) | A kind of aflatoxin detection device | |
| CN104395735B (en) | Method for carrying out optical measurement to the pyramid on veining silicon single crystal wafer | |
| JP5570771B2 (en) | Authenticity discrimination method and apparatus for paper sheets using terahertz light | |
| RU2549122C2 (en) | Sensor to inspect documents of value | |
| CN107144554A (en) | A kind of aflatoxin detection means | |
| CN206862897U (en) | A kind of aflatoxin detection means | |
| CN100451623C (en) | Fluorescence measuring equipment | |
| JP2003254856A (en) | Optical gas leakage detector and gas leakage detection vehicle | |
| Melgarejo et al. | Simultaneous determination of nickel, zinc and copper by second-derivative spectrophotometry using 1-(2-pyridylazo)-2-naphthol as reagent | |
| CN206292163U (en) | A kind of aflatoxin on-line measuring device of utilization Ultraluminescence spectrum | |
| CN107328745A (en) | A kind of Determination Methods of Aflatoxins and device | |
| CN105277131B (en) | Measuring device and measuring method of three-dimensional pore structure | |
| AU2559199A (en) | Method and apparatus for making absolute range measurements | |
| CN103353390B (en) | A kind of measuring method of air-gap type ahrens prism gummed error | |
| Rathod et al. | Validated UV spectrophotometric methods for the determination of anticancer drug erlotinib hydrochloride in bulk and tablet formulations | |
| EP3081901A1 (en) | Inspection method and device for inspecting a surface pattern | |
| CN107991266A (en) | A kind of on-line measuring device of aflatoxins | |
| US6850333B1 (en) | Optimized aperture shape for optical CD/profile metrology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |