CN106922694A - A kind of bactericidal composition - Google Patents

A kind of bactericidal composition Download PDF

Info

Publication number
CN106922694A
CN106922694A CN201511008836.7A CN201511008836A CN106922694A CN 106922694 A CN106922694 A CN 106922694A CN 201511008836 A CN201511008836 A CN 201511008836A CN 106922694 A CN106922694 A CN 106922694A
Authority
CN
China
Prior art keywords
picarbutrazox
fluoxastrobin
pyraclostrobin
plant
bactericidal composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201511008836.7A
Other languages
Chinese (zh)
Inventor
罗昌炎
詹姆斯·T·布里斯托
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Rotam Chemical Co Ltd
Original Assignee
Jiangsu Rotam Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Rotam Chemical Co Ltd filed Critical Jiangsu Rotam Chemical Co Ltd
Priority to CN201511008836.7A priority Critical patent/CN106922694A/en
Publication of CN106922694A publication Critical patent/CN106922694A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Soil Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a kind of bactericidal composition, the microbicide compositions include active component Picarbutrazox and Fluoxastrobin or pyraclostrobin, and the weight ratio of Picarbutrazox and Fluoxastrobin or pyraclostrobin is 50:1-1:50.The present invention carries out binary built by by Picarbutrazox and Fluoxastrobin or pyraclostrobin so that the present invention has obvious gain effect on preventing and treating crop by fungi, bacterial disease；In addition fungicidal spectrum is also expanded by binary built, reduces respective usage amount, reduce the risk that germ develops immunity to drugs.

Description

A kind of bactericidal composition
Technical field
The present invention relates to a kind of bactericidal composition, plant, crop or seed are more particularly, to protected from fungal disease Bactericidal composition.
Background technology
The current disease control for being agriculturally also easy to produce resistance, mixture is carried out using the different pesticide species of the mechanism of action It is optimal selection, if reasonable mixture ratio, significant synergistic effect can be produced, field control effect is substantially better than each list The effect of agent.Bactericide containing single-activity composition is usually present certain defect, continuous several times in agricultural disease preventing and treating Using not only making pathogen be also easy to produce the resistance to the action of a drug, and easily cause the pollution to food and environment, by fungicidal activity into point it Between rational mixture can overcome disadvantage mentioned above.Rational compounding makes the synergistic effect that active ingredient is produced, and can improve preventive effect, subtracts Few effective ingredient consumption, it is cost-effective, delay the drug-fast generation of pathogen, and then can mitigate or even avoid agricultural chemicals to food The pollution of thing and environment.
On pesticide activity, particularly to crop protection, one of key problem of research carried out in the technical field is Improve the performance in terms of performance, especially bioactivity and the performance for keeping within a certain period of time in terms of this activity.
Picarbutrazox, test code number NF-171；CAS：500207-04-5；Chemical formula is [6- [[[[(z)-(1- first Base -1H-TETRAZOLE -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- bases] t-butyl carbamate；Molecular structure Formula is：
Picarbutrazox is the oximino ether bactericide of Japanese Cao Da companies exploitation, and it has preferable to downy mildew and epidemic disease Prevention effect.Picarbutrazox is by known in CN02817805.X.
Pyraclostrobin（Pyraclostrobin）, also known as pyraclostrobin, its chemical name is：Methyl（N)-[[[1-(4- Chlorobenzene）The base of pyrazoles -3）- oxygen] -0- methoxyl groups]-N- methoxymethyl carbamates, its structural formula is：
Pyraclostrobin is the same with the mechanism of action of other synthesis strobin analogs, is a kind of mitochondrial respiratory inhibitor. It suppresses mitochondrial respiratory and acts on by preventing electron transmission between thin race pigment b and C1, prevents mitochondria from producing and providing Energy required for cell eubolism（ATP）, ultimately result in cell death.Pyraclostrobin has stronger suppression germ spore Sub- sprouting ability, has its lasting period of good inhibiting effect more long to mycelial growth in blade, and is lived with potential treatment Property.The compound is weaker to blade tip or phyllopodium conduction and fumigation action in blade, but conductive activity in plant is stronger. It is again a kind of hormone-type bactericide simultaneously, and crop can be made to absorb more nitrogen, promotes the growth of crop.In a word, pyrazoles ether bacterium Ester has protective effect, therapeutic action, Uptake and translocation and resistance of rainwater washing against performance, and range of application is wider.Pyraclostrobin It is mainly used in preventing and treating wheat, paddy rice, peanut, grape, vegetable Lay, banana, lemon, coffee, fruit tree, walnut, vegetables, tea tree, tobacco With the disease on ornamental plant, lawn and other field crops.Pyraclostrobin can be prevented and treated effectively by Ascomycetes, basidiomycetes Leaf blight, rust, powdery mildew, downy mildew, epidemic disease, anthracnose, scab, leaf that guiding principle, Fungi Imperfecti and Oomycete fungal cause The multiple diseases such as pinta, shot hole, brown spot, damping-off.Pyraclostrobin is applied to preventing and treating cereal crops disease, to cereal The disease of leaf portion and fringe grain has prominent prevention effect, and obvious effect of increasing production.Pyraclostrobin is right not only to crop safety Non-target organism safety, and it is friendly to user and environment safety.
Fluoxastrobin（Azoxystrobin）, its chemical name is：(E) -2- { 2- [6 (2- cyano-benzene oxygens) pyrimidine-4-yls Oxygen] phenyl } -3- methoxy-methyl acrylates, its structural formula is：
Fluoxastrobin is methoxy acrylate (Strobilurin) class sterilization pesticide.Fluoxastrobin is efficient, wide spectrum, to nearly all Mycota（Ascomycotina, Basidiomycotina, Mastigomycotina and Deuteromycotina）Disease such as powdery mildew, rust, grain husk is withered Disease, net blotch, downy mildew, rice blast etc. have good activity.Fluoxastrobin can be used for cauline leaf spraying, Seed Treatment, can also enter Row soil treatment.Fluoxastrobin is mainly used in cereal, paddy rice, peanut, grape, potato, fruit tree, vegetables, coffee, lawn etc..
Because the environmental requirement and economic requirement now to bactericide are constantly improve, such as to activity profile, toxicity, selection The requirement of property, rate of application, residual and favourable preparation feasibility, additionally asks due to being there may be in terms of such as drug resistance Topic.Therefore, exploitation is in some aspects lasting task better than the new bactericide of existing bactericide.
The content of the invention
It is an object of the invention to provide a kind of bactericidal composition, just reduced in the case where the reactive compound for reducing is using total amount and applied For activity profile with rate and improvement known compound Picarbutrazox and Fluoxastrobin or pyraclostrobin, the present invention is in drop Low activity compound is applied under total amount, and having to harmful fungoid improves activity, synergistic function.
We have found that simultaneously, i.e., Picarbutrazox and Fluoxastrobin or pyraclostrobin are jointly or separately applied, or Cause preferably to have been prevented and treated than each compound is administered alone using Picarbutrazox and Fluoxastrobin or pyraclostrobin successively Evil fungi.
The invention provides a kind of bactericidal composition, said composition is by by Picarbutrazox and Fluoxastrobin or pyrazoles Kresoxim-methyl carries out binary built so that the composition for obtaining has gain effect in prevention effect, and has expanded fungicidal spectrum, The multiplex effect of a medicine is served, effectively slows down or avoid germ to develop immunity to drugs.Astoundingly, Bactericidal composite of the invention The bactericidal activity of thing is more active plus and considerably higher than each reactive compound.In other words, exist it is unpredictable, truly deposit Cooperative effect, and be not only activity supplement.
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, cooperative effect is especially bright It is aobvious.But, the weight ratio of the reactive compound in bactericidal composition of the present invention can change within the specific limits.
A kind of bactericidal composition of the present invention is to take following technical scheme to realize：
A kind of bactericidal composition, it is characterised in that：Containing active component Picarbutrazox and Fluoxastrobin or pyraclostrobin, The percentage by weight of wherein Picarbutrazox and Fluoxastrobin or pyraclostrobin is 50:1-1:50, preferably 25:1-1:25, 10:1-1:10, more preferably 5:1-1:5.
The weight proportion of Picarbutrazox and Fluoxastrobin or pyraclostrobin in the present invention for example can also be 50: 1、45:1、40:1、35:1、30:1、25:1、20:1、15:1、10:1、5:1、1:1、1:5、1:10、1:15、1:20、1:25、1: 30、1:35、1:40、1:45、1:50。
Described bactericidal composition, it is characterised in that：The Picarbutrazox and Fluoxastrobin or pyraclostrobin quality Sum accounts for the 5%-90% of the Bactericidal composite amount of substance, more preferably more preferably 10%-80%, 20%-60%.
A kind of method for preventing and treating plant pathogen, pathogenic bacteria and/or its environment, Huo Zhezhi are acted on by bactericidal composition In thing, plant parts, seed, soil, region, material or space.
A kind of method for preventing and treating plant pathogen, Picarbutrazox and Fluoxastrobin or pyraclostrobin are administered simultaneously, Or apply respectively or sequential application.
A kind of bactericidal composition, comprising Picarbutrazox and Fluoxastrobin or pyraclostrobin and filler and/or surface Activating agent.
A kind of bactericidal composition, can be configured to any formulation for agriculturally allowing.The formulation of described bactericidal composition is Suspending agent, seed coat agent, suspension emulsion, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, Missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids.
Described bactericidal composition is used to prevent and treat Cereal, vegetables, clover, soybean, turf, timber, trees, fruit tree or garden The purposes of fungi and bacterium on skill plant.
The bactericidal composition is used to protect the purposes of plant propagation material and the plant organ for then growing.
The bactericidal composition is used to prevent and treat the purposes of fruit vegetables storing period disease.
The bactericidal composition be applied to needed for preventing and treating place preventing and treating soil cause a disease or saprophytic fungi and bacterium use On the way.
A kind of plant pathogenic fungi of control plant, plant part, plant propagation material and the plant organ for then growing Method, the method include by the amount of application of described bactericidal composition effective and basic plant-less toxicity with agronomy with seed at The methods such as reason, foliage applying, stem are applied, are impregnated with, instiling, pouring into a mould, spraying, spraying, dusting, distribution or smoke be administered to plant, Plant part, plant propagation material or plant are being grown or needed in the soil or cultivation medium for growing wherein.
Described bactericidal composition is in various crop plants such as banana, cotton, vegetable variety（Such as cucumber, beans, tomato And cucurbitaceous plant）, it is barley, dogstail, oat, coffee, potato, corn, fruit variety, rice, rye, soybean, grapevine, small A large amount of fungies are prevented and treated in wheat, ornamental plant, sugarcane and a large amount of seeds to be even more important.
Bactericidal composition of the invention has very strong activity to various phytopathogens, and can be to being drawn by phytopathogen The prevention and treatment of the plant disease for rising play very strong preventive effect.Bactericidal composition of the invention to such as Plasmodiophoromycetes, Oomycete, Chytridiomycetes, connect there is pole with the wide scope plant pathogenic fungi such as Gammaproteobacteria, Ascomycetes, Basidiomycetes, deuteromycetes Good activity.
Bactericidal composition of the invention also has extraordinary bactericidal performance, and it is thin to can be used for preventing and treating plant pathogenic Bacterium.Such as pseudomonadaceae, Rhizobiaceae, enterobacteriaceae, bar Cordycepps and Streptomycetaceae.
Bactericidal composition of the invention can be used as blade face fungicide in crop protection, can act also as fungicide use In seed dressing and as soil fungicide.
Bactericidal composite of the invention also can be used to prevent and treat the disease of fruit vegetables storing period.
Bactericidal composition of the invention is particularly suitable for the following phytopathogen of preventing and treating：
As oomycetes subclass, can be exemplified as, such as various crops such as the corruption of black leg of beet bacterium (Pythium ultium) Mould category bacterium；Such as potato blight bacterium (PhytophthoraiNfestaNs), botrytis cinerea (Phytophthora Capsici Phytophthora category bacterium)；Such as bacterium of downy mildew of cucumber (PseudoperoNospora cubeNsis), Humulus The Pseudoperonospora bacterium of Pseudoperonospora cubensis (PseudoperoNospora humuli)；Such as Plasmopara viticola (Plasmopara Viticola Plasmopara bacterium)；Such as Pseudoperonospora cubensis (PeroNospora brassicae), the green onion downy mildew of brassicaceous vegetable Germ (PeroNospora destructor), Peronospora bacterium of downy mildew of spinach bacterium (PeroNospora spiNaciae) etc..
As Ascomycetes, can be exemplified as, for example, such as the powdery mildew of powdery mildew of cereals bacterium (ErysiphegramiNis) Category bacterium；Such as the Sphaerotheca bacterium of greengrocery powdery mildew (Sphaerotheca fuligiNea)；Such as apple black star bacteria The Venturia bacterium of (VeNturia iNaequalis), pear cucumerinum (VeNturia Nashicola)；Such as net blotch of barley The PyreNophora category bacterium of bacterium (PyreNophorateres)；Such as wheat class spot disease (Cochliobolus sativus) Cochliobolus belongs to bacterium；Such as Sclerotinia bacterium of greengrocery hyphal cluster germ (SclerotiNia sclerotiorum).
As Basidiomycetes, can be exemplified as, for example, such as double spores of wheat leaf rust germ (PucciNiarecoNdita) Rust bacterium；Such as the Tilletia bacterium of bunt of wheat bacterium (Tilletia caries)；Such as loose smut of barley bacterium The Ustilago bacterium of (Ustilago Nuda) etc.
As Deuteromycotina, can be exemplified as, for example, such as the Phoma of lucid asparagus Phoma asparagi Sacc (Phomaasparagi) Bacterium；Such as the Septoria bacterium of wheat class crust rot bacterium (Septoria Nodorum)；Such as Colletotrichumlagenarium The Colletotrichum bacterium of (Colletotrichum lageNarium)；Such as wilt (Pyricularia oryzae) Pyricularia belongs to bacterium；Such as the Botrytis bacterium of greengrocery ash arrhizus bacteria (Botrytis ciNerea)；Such as apple spot fallen leaves Germ (AlterNaria mali), the Alternaria bacterium of tomato early blight bacterium (AlterNaria solaNi)；Such as beet Cercospora The Cercospora bacterium of germ (Cercospora beticola)；Such as peach black star germ (Cladosporium carpophilum) Blastocladia bacterium；Such as Rhizoctonia bacterium of Oryza sheath blight fungus (RhizoctoNia solaNi).
Suitable crop mainly includes field crop, such as corn, soybean, cotton, rape oilseeds, such as southern general rape (Brassica napus) (such as rape (canola)), overgrown with weeds green grass or young crops (Brassica rapa), leaf mustard (B.juncea) (such as mustard Sub (mustard)) and brassicacarinata (Brassica carinata), rice, wheat, beet, sugarcane, oat, brown wheat, greatly Wheat, broomcorn millet, triticale, flax, grapevine and the other fruit of various plants or vegetable crop, such as rose family (Rosaceaesp.) (for example, pip fruit, such as apple and pears, also drupe, such as apricot, cherry, almond and peach, berry such as strawberry), tea sugarcane Scarabaeidae (Ribesioidae sp.), Juglandaceae (Juglandaceae sp.), Betulaceae (Betulaceae sp.), Anacardiaceae (Anacardiaceae sp.), Fagaceae (Fagaceae sp.), Moraceae (Moraceae sp.), Oleaceae (Oleaceae Sp.), Actinidiaceae (Actinidaceae sp.), Lauraceae (Lauraceae sp.), Musaceae (Musaceae sp.) be (for example Banana tree and powder bajiao banana (plantings)), Rubiaceae (Rubiaceae sp.) (such as coffee), Theaceae (Theaceae Sp.), Sterculiaceae (Sterculiceaesp.), Rutaceae (Rutaceae sp.) (such as lemon, orange and grape fruit)；Solanaceae (Solanaceaesp.) (for example, tomato, potato, pepper, eggplant), Liliaceae (Liliaceae sp.), composite family (Compositiae sp.) (such as lettuce, globe artichoke and witloof-include root witloof (root chicory), hare's-lettuce (endive) Or common witloof (common chicory)), Umbelliferae (Umbelliferae sp.) (such as carrot, parsley, celery and block Rooted celery), Curcurbitaceae (Cucurbitaceae sp.) (for example cucumber-include pickled cucumber (pickling cucumber), south Melon, watermelon, cucurbit and muskmelon), green onion section (Alliaceae sp.) (such as onion and leek), Cruciferae (Cruciferae Sp.) (for example white cabbage, red cabbage, broccoli, cauliflower, brussels sprout, green vegetables, kohlrabi, radish, horseradish, solely Row dish, Chinese cabbage), pulse family (Leguminosaesp.) (such as peanut, pea and French beans-such as climning bean and broad bean), Chenopodiaceae (Chenopodiaceae sp.) (such as fodder beet, spinach sweet (spinach beet), spinach, beet root), Malvaceae (Malvaceae) (such as gumbo), Asparagaceae (Asparagaceae) (such as lucid asparagus)；Garden crop and forest crop； Ornamental plant；And the homologue of the genetic modification of these crops.
The preferably for example following disease of Bactericidal composite of the invention etc. is prevented and kill off：
The brown spot (Cercospora beticola) of " beet ", black root（Aphanomyces cochlloides）, root-rot Disease（Thanatephorus cucumeris）, leaf rot（Thanatephorus cucumeris）；
The brown spot of " peanut "（Mycosphaerella arachidis）, black spot（Mycosphaerella berkeleyi）；
The powdery mildew of " cucumber "（Sphaerotheca fuliginea）, downy mildew（Pseudoperonospora cubensis）, blight dis-ease（Mycosphaerella melonis）, dead arm（Fusarium oxysporum）, sclerotiniose （Sclerotinia sclerotiorum）, gray mold（Botrytis cinerea）, anthracnose（Colletotrichum orbiculare）, scab（Cladosporium cucumerinum）, brown spot（Corynespora cassicola）, seedling Damping-off（Pythium debaryanam, Rhizoctonia solani Kuhn）, bacterial leaf spot（Pseudomonas syringae pv.Lecrymans）；
The gray mold of " tomato "（Botrytis cinerea）, leaf mold（Cladosporium fulvum）, late blight （Phytophthora infestans）；
The gray mold of " eggplant "（Botrytis cinerea）, bacterial blight（Corynespora melongenae）, powdery mildew （Erysiphe cichoracearum）, suede bacterial plaque disease（Mycovellosiella nattrassii）；
The gray mold of " strawberry "（Botrytis cinerea）, powdery mildew（Sohaerotheca humuli）, anthracnose （Colletotrichum acutatum、Colletotrichum fragariae）, epidemic disease（Phytophthora cactorum）；
The neck rot of " onion "（Botrytis allii）, gray mold（Botrytis cinerea）, hickie leaf blight （Botrytis squamosa）, downy mildew（Peronospora destructor）；
The root knot of " cabbage "（Plasmodiophora brassicae）, soft rot（Erwinia carotovora）, frost Mildew（Peronospora parasitica）；
The sclerotiniose of " French beans "（Sclerotinia sclerotiorum）, gray mold（Botrytis cinerea）；
The powdery mildew of " apple "（Podosphaera leucotricha）, scab（Venturia inaequalis）, spend corruption Disease（Monilinia mali）, diplostomiasis（Mycosphaerella pomi）, rot（Valsa mali）, spot defoliation （Alternaria mali）, rust（Gymnosporangium yamadae）, ring spot（Botryosphaeria berengeriana）, anthracnose（Glomerella cingulata、Colletotrichum acutatum）, brown spot （Diplocarpon mali）, fly pinta（Zygophiala jamaicensis）, sooty mould（Gloeodes pomigena）；
The powdery mildew of " persimmon "（Phyllactinia kakicola）, anthracnose（Gloeosporium kaki）, angle spot fallen leaves Disease（Cercospora kaki）；
The brown rot of " peach "（Monilinia fructicola）, scab（Cladosporium carpophilum）, intend stem Point mildew（Phomopsis sp.）；
The brown rot of " cherry "（Monilinia fructicola）；
The gray mold of " grape "（Botrytis cinerea）, powdery mildew（Uncinula necator）, late blight （Glomerella cingulata、Colletotrichum acutatum）, downy mildew（Plasmopara viticola）, it is black Acne disease（Elsinoe ampelina）, brown spot（Pseudocercospora vitis）, black rot（Guignardia bidwellii）；
The scab of " pears "（Venturia nashicola）, rust（Gymnosporangium asiaticum）, black spot （Alternaria kikuchiana）, ring spot（Botryosphaeria berengeriana）, powdery mildew （Phyllactinia mali）；
The zonate spot of " tea "（Pestalotia theae）, anthracnose（Colletotrichum theae-sinensis）；
The shot hole of " citrus "（Elsinoe fawcetti）, penicilliosis（Penicillium italicum）, green mould （Penicillium digitatum）, gray mold（Botrytis cinerea）, diplostomiasis（Diaporthe citri）, ulcer Disease（Xanthomonas campestris pv.Citri）；
The powdery mildew of " wheat "（Erysiphe graminis f.sp.tritici）, head blight（Gibberella zeae）, leaf Rust（Puccinia recondita）, brown snow mold（Pythium iwayamai）, snow red rot （Monographella nivalis）, wheat basal stem rot（Pseudocercosporella herpotrichoides）, leaf it is withered Disease（Septoria tritici）, glume blight（Leptosphaeria nodorum）, the rotten sclerotium oryzicola of snow（Typhula incarnata）, snow big grain sclerotinia rot（Myriosclerotinia borealis）, damping-off（Gaeumanomyces graminis）；
The stripe disease of " barley "（Pyrenophora graminea）, clouding disease（Rhynchosporium secalis）, dissipate it is black Fringe disease（Ustilago tritici、U.nuda）；
The rice blast of " rice "（Pyricularia oryzae）, banded sclerotial blight（Rhizoctonia solani）, bakanae disease （Gibberella fujikuroi）, flax leaf spot（Cochliobolus niyabeanus）, seedling blight（Pythium graminicola）, bacterial leaf-blight（Xanthomonas oryzae）, bacillary seedling blight（Burkholderia plantarii）, brown streak（Acidovorax avenae）, the withered phytosis of paddy（Burkholderia glumae）
The sclerotiniose of " tobacco "（Sclerotinia sclerotiorum）, powdery mildew（Erysiphe cichoracearum）；
The gray mold of " tulip "（Botrytis cinerea）；
The snow big grain sclerotinia rot of " Western Korea lawn grass "（Sclerotinia borealis）, samping off（Pythium aphanidermatum）；
The powdery mildew of " orchard grass "（Erysiphe graminis）；
The purple spot of " soybean "（Cercospora kikuchii）, downy mildew（Peronospora Manshurica）, cane blight （Phytophthora sojae）；
Potato, tomato late blight（Phytophthora infestans）.
Bactericidal composition of the invention can be good preventing and treating cause pathogen and the medicament drug-fast bacteria of following disease：Paddy rice Seedbed damping-off, bakanae disease；Disease in cotton seedling stage such as damping-off, anthracnose, samping off, red rot；Vegetable nursery bed disease is for example sudden Sick anthracnose, damping-off, the phytophthora root rot of tomato, gray mold, leaf rust, spot disease, anthracnose and early phytophthora root rot；The frost of cucumber Mildew, powdery mildew, gray mold, anthracnose, withered rattan disease, black spot and brown spot；The anthracnose and gray mold of strawberry；Pumpkin it is white Powder disease, anthracnose and withered rattan disease；The hickie leaf blight of onion, black spot, gray mold and Mild Powder；The powdery mildew of eggplant, grey mold Disease and bacterial blight；The black spot of shallot；The Leucoplakia and black spot of Chinese cabbage；The sclerotiniose and gray mold of green pepper；Cucumber it is white Powder disease, anthracnose and withered rattan disease；The powdery mildew of muskmelon, anthracnose and withered rattan disease；The gray mold of lettuce；The black spot and ash of red bayberry Mildew；The powdery mildew of persimmon, anthracnose, gray mold and defoliation；The gray mold of oranges and tangerines；The scab of pears, black spot and early epidemic Mildew；The gray mold of grape, anthracnose, brown spot, black rattan are sick, withered rattan is sick, viscous rot and the swollen disease of branch；The scab of peach, ash Pinta, anthracnose；The scab of apple, zonate spot, early phytophthora root rot, brown spot, coal point disease, coal spot disease, diplostomiasis and brown rot； The stem wilt of mung bean, anthracnose, gray mold and sclerotiniose；The anthracnose of Kidney bean, gray mold and sclerotiniose；The ring spot of broad bean； Purple spot of soybean；The powdery mildew of tobacco, the brown spot of beet and spot disease；The anthracnose of tea, zonate spot and the withered disease of sprouting and Gray mold；Spend the plant diseases such as scab, anthracnose, gray mold and the powdery mildew of class.Bactericidal composition of the invention is particularly Preventing and treating tomato that can be good, cucumber, eggplant, strawberry, grape, the epidemic disease of peach and oranges and tangerines, powdery mildew and the gray mold cause of disease Phytopathogen and medicament drug-fast bacteria.
Bactericidal composition of the invention is especially suitable for preventing and treating apple, banana, grape, oranges and tangerines, mango, paddy rice, wheat, jade Downy mildew on rice, potato, soybean, tomato, capsicum, cucumber, eggplant, grape, early blight, late blight, balck shank, scab, Leaf spot, anthracnose, powdery mildew, wilt disease, damping-off, samping off.
The crop of bactericidal composition treatment of the invention is such as but not limited to cereal, vegetables, clover, soybean, turf, wood Material, trees, fruit tree or gardening plant.
Fungicidai mixtures of the invention can also be used to prevent and treat fruit vegetables storing period disease.The fruit for for example being caused by following pathogen It is real to rot：
Aspergillus bacterium, such as aspergillus flavus；
Botrytis（Botrytis）Strain, such as Botrytis cinerea（Botrytis cinerea）;
Penicillium（Penicillium）Strain, such as penicillium expansum（Penicillium expansum）With P.purpurogenum;
Sclerotinia（Sclerotinia）Strain, such as sclerotinite（ Sclerotinia sclerotiorum）;
Verticillium（Verticilium）Strain, such as black and white wheel branch spore（Verticilium alboatrum）.
Bactericidal composition of the invention be also particularly well suited for preventing and treating fruit vegetables storing period disease blossom-end rot, green mould, penicilliosis, Anthracnose.
Fungicidai mixtures of the invention can be additionally used in preventing and treating kind and pass and soil-borne disease.The kind for for example being caused by following pathogen Pass and native rotten and wilting disease and the seedling disease for passing：
Alternaria category kind, such as rape give birth to rod method (Alternaria brassicicola);
Aphanomyces (Aphanomyce) category kind, such as mould (Aphanomyces of Kidney bean silk capsule
euteiches) ;
Ascochyta (Ascochyta) category kind, such as Ascochyta lentis;
Aspergillus, such as aspergillus flavus;
Cladosporium category kind, such as draft branch spore (Cladosporium herbarum);
Cochliobolus category category kind, such as standing grain cochliobolus;
(conidial form:Drechslera, Syn:The compacted spore bacterium of length);
Colletotrichum category kind, such as hair core anthrax-bacilus (Colletotrichum coccodes);
Fusarium category kind, such as yellow fusarium;
Gibberella category kind, such as beautiful another name for Sichuan Province multitude is red mould;
Shell ball spore category (Macrophomina) category kind, such as Kidney bean shell ball spore (Macrophomina
phaseolina) ;
Small setting-out shell belongs to strain, for example, avenge rotten small setting-out shell;
Penicillium category kind, such as penicillium expansum;
Phaeosphaeria strains, such as Phaeosphaeria nodorum;
Phoma (Phoma) category kind, such as black embryo Phoma sp (Phoma lingam);
Phomopsis (Phomopsis), such as soybean Phomopsis (Phomopsis sojae);
Phytophthora category kind, such as Phytophthora cactorum (Phytophthora cactorum);
Nuclear cavity Pseudomonas category kind, such as wheat class nuclear cavity bacteria (Pyrenophora graminea);
Pyricularia Sacc. (Pyricularia) category kind, such as Magnaporthe grisea (Pyricularia oryzae);
Pythium kind, such as Pythium ultimum;
Rhizoctonia category kind, such as Rhizoctonia solani Kuhn;
Rhizopus (Rhizopus) category kind, such as Rhizopus oryzae (Rhizopus oryzae);
Bulbil category (Sclerotium) category kind, such as Sclerotium rolfsii (Sclerotium rolfsii);
Core coral Pseudomonas (Typhula) category kind, such as meat spore core coral bacterium (Typhula incarnata);
Verticillium (Verticillium) category kind, such as verticillium dahliae (Verticillium dahliae).
Bactericidal composition of the invention also has excellent bactericidal effect to the bacterium of existing bactericide display tolerance.For example Ash arrhizus bacteria（Botrytis cinerea）, sugar beet leaf spot bacteria（Cercospora beticola）, apple black star bacteria （Venturia inaequalis）, pear cucumerinum（Venturia nashicola）Deng.
A kind of plant pathogenic fungi of control plant, plant part, plant propagation material and the plant organ for then growing Method, including by the amount of application of the bactericidal composition of the invention effective and basic plant-less toxicity with agronomy with seed at The methods such as reason, foliage applying, stem are applied, are impregnated with, instiling, pouring into a mould, spraying, spraying, dusting, distribution or smoke be administered to plant, Plant part, plant propagation material or plant are being grown or needed in the soil or cultivation medium for growing wherein.
Bactericidal composition of the invention can be spread by Seed Treatment, cauline leaf, soil application or it is used for ponds etc. and The prevention and treatment of the various diseases for being produced when cultivation includes the agriculture garden crop of flowers, lawn, herbage.
The present invention provides a kind of method for preventing and treating plant pathogen, and bactericidal composition is acted on into pathogenic bacteria and/or its ring In border, or plant, plant parts, plant propagation material, soil, region, material or space.
Bactericidal composition of the invention can be used for the plant device protected plant part, plant propagation material and then grow Official.
Bactericidal composition of the invention, can process all plants and plant part." plant " refers to all plants and plant Population, such as preferably with undesirable wild plant, cultivated plant and plant variety (regardless of whether receiving plant variety or plant Cultivate the protection of people's right).Cultivated plant and plant variety can be the plants obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique method, such as using dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Above and below the ground part and organ, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.Crop and nourish and generate and case of propagation material, for example, transplant, bulb, rhizome, running roots Plant part is fallen within seed.
Term " plant propagation material " is interpreted as referring to all plant parts for having a fertility, such as seed, and it can be used In breeding the latter, and vegetable matter such as cuttage bar or stem tuber（Such as potato）.Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.Treat The germination plant and effective plant suppressed after being germinateed from soil or after emerging.Young plant can before transplantation by being impregnated into Row whole or Local treatment are protected.
Currently preferred propagating materials is seed.The method of Seed Treatment, for example, can there are, diluent liquid or solid The medicament of shape or seed is directly immersed in liquid condition solution the method for making medicament be impregnated with seed without dilution, by solid Medicament or liquid preparation are mixed with seed, be coated treatment make the surface of the seed adhere to medicament method, plantation Simultaneously near seed sprinkling etc. method.
Plant part and the plant organ for then growing are by any of the plant propagation material such as plant of seed generation Part.Plant part, plant organ and plant can also be benefited from and be applied to plant propagation material institute by by bactericidal composition The pathogenic damage protection of acquisition.The plant organ grown behind certain plants part and some places can also regard plant propagation as Material, its own can be applied with bactericidal composition（Or treatment）；So as to by the plant part through processing and the plant through processing Plant, other plant parts and the other plant organs that organ is produced can also be benefited from and applied by by bactericidal composition With.
Bactericidal composition of the invention can also be used to prevent or control soil or cultivate various in medium causing a disease or saprophytic Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria（Alternaria spp.）, Ascochyta (Ascochyta spp.), Botrytis cinerea（Botrytis cinerea, Cercospora（Cercospora spp., ergot （Claviceps purpurea）, standing grain cochliobolus（Cochliobolus sativus）, Colletotrichum（colletotrichum Spp., Epicoccum（Epicoccum spp., F.graminearum schw（Fusarium graminearum）, rice evil seedling chain spore （Fusarium moniliforme）, fusarium oxysporum（Fusarium oxysporum, fusarium moniliforme（Fusarium proliferatum）, fusariun solani (Fusarium solani) ties up glue chain spore (Fusarium subglitinans), long compacted Spore belongs to (Helminthosporium spp), rotten microtorr bacterium (Microdochium nivale) of snow, Penicillium (Pencillium Spp), Phoma (Phoma spp.), wheat class nuclear cavity bacteria (Pyrenophora graminea), rice blast Pyricularia Sacc. (Pyricularia oryzae), Rhizoctonia solani Kuhn (Rhizoctonia solani), Rhizoctonia cerealis (Rhizoctonia Cerealis), Sclerotinia (Sclerotinia spp.), Septoria (Septoria spp.), silk axle smut (Sphacelotheca reilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula Incarnate), hidden bar smut (Urocystis occulta), Ustilago (Ustilago spp.) or Verticillium (Verticillium spp.)。
Soil germ has rhizoctonia solani, sickle-like bacteria, phytophthora, bacterium of dampinging off, root rot, pythium spp, botrytis cinerea, soft-rot fungi Deng.In general, soil germ can produce a large amount of thalline, as long as condition is favourable to pathogen growth development, host is sense Disease, germ just with amount reproduction and can infect host, and in the presence of susceptible host, these germs can just enter lasting The phase of causing a disease, the amount reproduction diffusion with the continuous cropping of crop, but nutrient is depleted or edaphic condition such as temperature, humidity etc. afterwards When unfavorable to germ, germ can enter rest period again.When susceptible host does not exist, soil-borne disease bacterium can also survive in soil Get off, in addition to soil germ has extensive host range, moreover it is possible to survived on the root surface of non-host or the broken branches and fallen leaves, with it It is undivided with saprophytic competitiveness.But different germs are discrepant, as sickle-like bacteria almost can be unlimited in soil Phase survives.
Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, for example：Soil, water etc., Specific raw material can using such as sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Wood chip, bark etc..
To the method in soil using medicament, for example, liquid preparation is diluted in water or do not diluted and be directly applied to plant The medium method in the root of body or the rice seedling bed of seedling, the side in the rice seedling bed of the root of plant or seedling is disseminated to by granule Method has the method that pulvis, water dispersible granules etc. are sprayed in soil and are integrally mixed with soil prior to seeding, and sowing is preceding or plants In being sprayed on implantation hole, seed furrow after pulvis, water-dispersible grain dilution agent before kind plant, in method sowed etc..
Another object of the present invention, there is provided a kind of control plant, plant part, plant propagation material and then grow The method of the plant pathogenic fungi of plant organ, the method include by bactericidal composition of the invention with agronomy it is effective and substantially without Phytotoxic amount of application is applied with Seed Treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution Or the method such as smoke is administered to plant, plant part, plant propagation material or plant and is growing or needing what is grown wherein In soil or cultivation medium.
Bactericidal composition of the invention can be applied by different processing methods, and these methods are for example：
- liquid comprising the bactericidal composition is sprayed onto the aerial part of the plant；
- dusting, mixes particle or powder in soil, in surrounding plants sprinkling, and in tree injection or the situation of smearing Under；
- seed of plant is coated or film coated.
- for postharvest fruit and vegetable anti-corrosive fresh-keeping when, 200-2000 times of liquid of usual dilute with water, leaching fruit after leach.
The present invention provides a kind of method for preventing and treating plant pathogen, can treat, prevent or method of eradication.
Bactericidal composition of the invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid The dosage forms such as agent, granule, seed coat agent are used, its amount of application, mixing ratio, meteorological condition according to active ingredient, medicament Form, Dressing date, application process, using place, prevent and kill off the different and variant of object noxious livings, objective crop etc..
A kind of method for preventing and treating plant pathogen, Picarbutrazox and Fluoxastrobin or pyraclostrobin are administered simultaneously, Or apply respectively or sequential application.
Treatment in accordance with the present invention is there may be super plus (" collaboration ") effect.For example, sterilized group used according to the present invention The rate of application of compound and/or widen its field of activity and/or increase its activity, it is possible to obtain following effect：More preferable plant Growth, the tolerance to high temperature or low temperature increases, and the tolerance to arid or water or soil salt content increases, and performance of blooming is carried It is high, it is easier to harvest, the maturation of quickening, yield rate higher, bigger fruit, plant height higher, the color of leaf is more It is green, to bloom earlier, the quality or nutritive value of the product of results are higher, and sugared concentration is higher in fruit, the storage of the product of results More preferably, these benefits have exceeded the effect actually estimated for stability and/or processability.
Processing method of the invention can be additionally used in process propagating materials such as stem tuber or rhizome, and can be used for treatment seed, Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for processing root.The present invention Processing method can also be used for processing the aerial part of plant such as about the doing of plant, stem or stalk, leaf, flower and fruit.
- generally for leaf portion treatment：0.1-10000g/ha, preferably 10-1000 g/ha, more preferably 50-500 g/ha；It is right For dipping or administration of instiling, the dosage can also even be reduced, particularly when using inert base such as asbestos or pearl During rock；
- for Seed Treatment：2-5000g/100kg seeds, preferably 3-1000g/100kg seeds；
- for soil or treatment used for ponds：0.1-10000g/ha, preferably 1-1000g/ha；
- fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, leached after leaching fruit.
Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to actual feelings during actual administration Condition and needs, the seriousness especially in accordance with the property and germ of pending plant or crop adjust rate of application.
Picarbutrazox of the invention is combined with Fluoxastrobin or pyraclostrobin/co-administered.Including separating, successively Or Picarbutrazox and Fluoxastrobin or pyraclostrobin is administered simultaneously.Preferably, the Picarbutrazox and Fluoxastrobin Or pyraclostrobin is combined as the form of the composition comprising Picarbutrazox and Fluoxastrobin or pyraclostrobin.
Composition of the invention can be based on dosage form, i.e., each material is blended in composition, composition into Point can also unit dose form provide, use it is preceding mix in bucket or tank, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.
As a further improvement on the present invention, bactericidal composition of the invention can be configured to any agent for agriculturally allowing Type.
As a further improvement on the present invention, the formulation of bactericidal composition of the invention is suspending agent, seed coat agent, wettable It is pulvis, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, super Low capacity liquor, suspension emulsion.
In bactericidal composition of the present invention, comprising Picarbutrazox and Fluoxastrobin or pyraclostrobin, filler And/or surfactant.
The content of bactericidal composition of the present invention, wherein Picarbutrazox and Fluoxastrobin or pyraclostrobin is accounted for The 5%-90% of bactericidal composition.
The content of described bactericidal composition, wherein Picarbutrazox and Fluoxastrobin or pyraclostrobin accounts for sterilized group The 10%-80% of compound.
The content of described bactericidal composition, wherein Picarbutrazox and Fluoxastrobin or pyraclostrobin accounts for sterilized group The 20%-60% of compound.
According to the present invention, term " filler " refers to can be combined with reactive compound or combines to make it easier for being administered to Object（Such as plant, crop or careless class）Natural or synthetic organic or inorganic compound.Therefore, the filler is preferably It is inert, at least should be agricultural acceptable.The filler can be solid or liquid.
The nonactive medium that can be used in the present invention both can be that solid can also be liquid, can be as solid matchmaker What dielectric material was used has for example：Phyteral powdery type（Such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut The particle of shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem, extracts residue after plant essence etc.）, paper , sawdust, crush synthesized polymer body, the clay class of synthetic resin etc.（Such as kaolin, bentonite, acid china clay etc.）, talcum powder Class.Silica（Such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates）, activated carbon, natural mineral matter class（Float stone, Lv Po Thread stone and zeolite etc.）, fire diatomite, sand, plastics medium etc.（Such as polyethylene, polypropylene, polyvinylidene chloride etc.）, chlorination The chemical fertilizer of inorganic mineral powder, ammonium sulfate, ammonium phosphate, urea, the ammonium chloride of potassium, calcium carbonate, calcium phosphate etc. etc., soil Fertilizer, these materials can be used alone or two or more is mixed.
Can be selected in llowing group of materials as liquid media materials'use, such as water, alcohol type（Such as first Alcohol, ethanol, isopropanol, butanol, ethylene glycol etc.）, ketone（Such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng）, ethers（Such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.）, aliphatic hydrocarbon class（Such as kerosene, Mineral oil etc.）, aromatic hydrocarbons class（Such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro fat Fat hydrocarbon, chlorobenzene, etc.）, halogenated hydrocarbon class, amide-type, sulfone class, dimethyl sulfoxide (DMSO), mineral and vegetable oil, animal oil etc..
To emulsify active ingredient compound, dispersion and/or soak, it is possible to use surfactant for example can be with Enumerate AEO, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the fatty acid ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total to Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as thing, alkyltaurate, protein hydrolysate.Suitable oligosaccharide thing or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or its with for example（It is polynary）Alcohol or（It is polynary）The combination of amine.
To make active ingredient compound dispersion stability, attachment and/or combining, such as xanthans, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid（Such as cephalin and lecithin）With And the adjuvant such as synthetic phospholipid, bentonite, sodium lignin sulfonate.
The wherein optional spent glycol of antifreezing agent, propane diols, glycerine, sorbierite.As the deflocculant of suspension product The adjuvant of such as naphthalene sulfonic acid polymer, polymeric phosphate etc. can be used.
Organic silicon defoamer can be used as defoamer.
The colouring agent that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff：Alizarin dyes, azo dyes and metallized phthalocyanine dye；And trace element, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.
Optionally, can also include other annexing ingredients, for example protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.
The reactive compound can be mixed and prepared by the preparation of the invention by known way with conventional additives. The conventional additives such as conventional extender and solvent or diluent, emulsifying agent, dispersant, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, drier and colouring agent, stabilizer, pigment, defoamer, preservative, increasing can also be included Thick dose, water and other processing aids.
Bactericidal composition of the invention not only includes can be by suitable equipment as spraying or dusting equipment are applied to immediately Pending object, and the concentration commercial composition for needing to be diluted is additionally included in before being applied to object.
It is of the invention to be applied with other active ingredient combinations containing Picarbutrazox and Fluoxastrobin or pyraclostrobin With for example for expanding activity profile or preventing from forming resistance.Described other active components such as fungicide, bactericide, draw Lure, insecticide, acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..
Reactive compound Picarbutrazox and Fluoxastrobin or pyraclostrobin can be administered simultaneously, or apply respectively, or phase Order when administration, separate administration is on the result prevented and treated generally without influence.
Composition of the invention can be based on dosage form, i.e., each material is blended in composition, composition into Point can also unit dose form provide, use it is preceding mix in bucket or tank, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.
In the case where reactive compound is reduced using total amount, have to harmful fungoid improves activity to bactericidal composition of the invention, Synergistic function.And it is excellent that bactericidal composition of the invention shows that the bacterium of tolerance also has to existing bactericide Bactericidal effect.
A kind of bactericidal composition of the present invention, said composition is by by Picarbutrazox and Fluoxastrobin or pyraclostrobin Carry out binary built so that the composition for obtaining has gain effect in prevention effect, and has expanded fungicidal spectrum, serves The multiplex effect of one medicine, effectively slows down or avoids germ to develop immunity to drugs.The bactericidal activity of bactericidal composition of the invention is than each The active of individual reactive compound adds and considerably higher.In other words, there is unpredictable, necessary being cooperative effect, and Not exclusively active supplement.
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, cooperative effect is especially bright It is aobvious.But, the weight ratio of the reactive compound in bactericidal composition of the present invention can change within the specific limits.
Can have greater activity and the more long bactericidal composition of activity holding the invention provides a kind of.This sterilized group Compound has relatively low-dose, while the toxicity of the bactericidal composition is relatively low, fruits and vegetables and seed with fungal disease is carried out Control.
Specific embodiment
The invention will be further elaborated to first pass through specific example of formulations below.
Example of formulations
Embodiment 1：40% Picarbutrazox Fluoxastrobin wettable powders
Picarbutrazox 10%, Fluoxastrobin 30%, lignosulfonates 6%, spaonin powder 4%, attapulgite adds to 100%, will Aforementioned formula material mixes, the uniform stirring in stirred tank, is remixed after airslide disintegrating mill uniformly, that is, be obtained 40% Picarbutrazox Fluoxastrobin wettable powders.
Embodiment 2：50% Picarbutrazox pyraclostrobin wettable powders:
Picarbutrazox 5%, pyraclostrobin 45%, naphthalene sulfonic acid-formaldehyde condensation product 8%, tea seed cake 5%, kaolin adds to 100%, Aforementioned formula material is mixed, the uniform stirring in stirred tank, be well mixed after airslide disintegrating mill, be obtained 50% Picarbutrazox pyraclostrobin wettable powders.
Embodiment 3：51% Picarbutrazox pyraclostrobin wettable powders
Picarbutrazox 1%, pyraclostrobin 50%, AEO 7%, wetting and penetrating agent F 5%, diatomite adds To 100%, aforementioned formula material is mixed, the uniform stirring in stirred tank, be well mixed after airslide disintegrating mill, be obtained 51% Picarbutrazox pyraclostrobin wettable powders.
Embodiment 4：32% Picarbutrazox azoxystrobin water dispersible granules
Picarbutrazox 30%, Fluoxastrobin 2%, alkyl benzene sulfonate calcium salt 8%, Nekal BX 6%, ammonium sulfate 2%, bentonite adds To 100%, aforementioned formula material is uniformly mixed, with micro jet, through mediating, being subsequently adding fluidized-bed granulation dryer In granulated, dry, sieve after be mixed to prepare 32% Picarbutrazox azoxystrobin water dispersible granules through sampling analysis.
Embodiment 5：60% Picarbutrazox azoxystrobin water dispersible granules
Picarbutrazox 20%, Fluoxastrobin 40%, aliphatic acid polyethenoxy ether 7%, lauryl sodium sulfate 4%, aluminium chloride 2.8%, kaolin adds to 100%, and aforementioned formula material is uniformly mixed, with micro jet, through mediating, being subsequently adding fluidisation Bed granulating and drying machine in granulated, dry, sieve after be mixed to prepare 60% Picarbutrazox Fluoxastrobins through sampling analysis Water dispersible granules.
Embodiment 6：90% Picarbutrazox pyraclostrobin water dispersible granules
Picarbutrazox 20%, pyraclostrobin 70%, octylphenol polyethylene oxyethylene group ether sulfate 1%, DBSA Sodium 1%, sodium carbonate 1%, white carbon adds to 100%, and aforementioned formula material is uniformly mixed, with micro jet, through mediating, so Add afterwards granulated in fluidized-bed granulation dryer, dry, sieve after through sampling analysis i.e. prepared 90% Picarbutrazox pyraclostrobin water dispersible granules.
Embodiment 7：45% Picarbutrazox pyraclostrobin suspending agents
Picarbutrazox 40%, pyraclostrobin 5%, polycarboxylate 6%, silicone oil 0.3%, xanthans 0.9%, diethylene glycol (DEG) 2.5%, Deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to active ingredient is crossed, by high speed shear It is well mixed, active ingredient is added, ball milling 2 ~ 3 hours in ball mill make particle diameter all below 5 μm, that is, be obtained 45% Picarbutrazox pyraclostrobin suspending agents.
Embodiment 8：55% Picarbutrazox Fluoxastrobin suspending agents
Picarbutrazox 50%, Fluoxastrobin 5%, naphthalene sulfonic acid-formaldehyde condensation product 6%, hydroxyethyl cellulose 0.7%, silicone chemical combination Thing 0.1%, polyethylene glycol 2%, deionized water adds to 100%, remaining group after thickener and antifreezing agent are mixed in addition to active ingredient Point, it is well mixed by high speed shear, add active ingredient, ball milling 2 ~ 3 hours in ball mill, make particle diameter all 5 μm with Under, that is, 55% Picarbutrazox Fluoxastrobin suspending agents are obtained.
Embodiment 9：40% Picarbutrazox pyraclostrobin suspending agents
Picarbutrazox 15%, pyraclostrobin 25%, lignosulfonates 8%, Magnesiumaluminumsilicate 1%, silicone 0.2%, the third three Alcohol 2.8%, deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to active ingredient, by a high speed Shear-mixed is uniform, adds active ingredient, and ball milling 2 ~ 3 hours in ball mill make particle diameter all below 5 μm, that is, be obtained 40% Picarbutrazox pyraclostrobin suspending agents.
Embodiment 10：20% Picarbutrazox Fluoxastrobin suspension emulsions
Picarbutrazox 10%, Fluoxastrobin 10%, SOLVESSOTM100 10%, alkyl benzene sulfonate calcium salt 7%, methylcellulose 1.2%, propane diols 2.4%, C8 ~ 10 aliphatic alcohols 0.4%, Tween 80 7%, water adds to 100%；Add in the continuous phase containing Fluoxastrobin Enter the suspended phase of finely ground Picarbutrazox, be mixed to prepare 20% Picarbutrazox Fluoxastrobin suspension emulsions.
Embodiment 11：20% Picarbutrazox pyraclostrobin suspension emulsions
Picarbutrazox 5%, pyraclostrobin 15%, lignosulfonates 6%, xanthans 0.8%, ethylene glycol 2.5%, SOLVESSOTM100 15%, silicone oil 0.2%, 600# phosphates 5%, water adds to 100%.In the continuous phase of pyraclostrobin-containing The suspended phase of finely ground Picarbutrazox is added, the outstanding breast of 20% Picarbutrazox pyraclostrobins is mixed to prepare Agent.
Embodiment 12：30% Picarbutrazox Fluoxastrobin suspension emulsions
Picarbutrazox 20%, Fluoxastrobin 10%, naphthalene sulfonic acid-formaldehyde condensation product 7%, spaonin powder 3%, phenolic resin 1.1%, three is sweet Alcohol 2%, silicone 0.1%, agriculture breast 400#6%, water adds to 100%, is added in the continuous phase containing Fluoxastrobin finely ground The suspended phase of Picarbutrazox, is mixed to prepare 30% Picarbutrazox Fluoxastrobin suspension emulsions.
Embodiment 13：10% Picarbutrazox azoxystrobin microemulsions
Picarbutrazox 2%, Fluoxastrobin 8%, N- methyl pyrrolidones 6%, oxirane-propylene oxide block copolymer 4%th, alkyl biphenyl ether disulfonic acid magnesium salts 5%, silicone compound 0.4%, toluene 3%, propane diols 2%, epoxychloropropane 1.1%, go Ionized water adds to 100%, is made 10% Picarbutrazox azoxystrobin microemulsions.
Embodiment 14：5% Picarbutrazox pyraclostrobin microemulsions
Picarbutrazox 1%, pyraclostrobin 4%, cyclohexanone 5%, agriculture breast 500#4%, Nongru-700 #5%, C8 ~ 10 fatty alcohols Class 0.2%, ethyl acetate 2.5%, polyethylene glycol 2%, tributyl phosphate 1.4%, deionized water add to 100%, are made 5% Picarbutrazox pyraclostrobin microemulsions.
Embodiment 15：50% Picarbutrazox azoxystrobin water dispersible granules
Picarbutrazox 40%, Fluoxastrobin 10%, octylphenol polyethylene oxyethylene group ether sulfate 1%, neopelex 1%, sodium carbonate 1%, white carbon adds to 100%, and aforementioned formula material is uniformly mixed, with micro jet, through mediating and then Add granulated in fluidized-bed granulation dryer, dry, sieve after through i.e. prepared 50% Picarbutrazox of sampling analysis Azoxystrobin water dispersible granule.
Embodiment 16：10% Picarbutrazox azoxystrobin water emulsions
Picarbutrazox 5%, Fluoxastrobin 5%, 600# phosphates 4%, propyl alcohol 7%, agriculture breast 600#3%, xanthans 0.7%, the third two Alcohol 2.4%, toluene 4%, deionized water add to 100%, are made 10% Picarbutrazox azoxystrobin water emulsions.
Embodiment 17：11% Picarbutrazox Fluoxastrobin missible oil
Picarbutrazox 1%, Fluoxastrobin 10%, ethoxylated castor oil 5%, calcium dodecyl benzene sulfonate 3%, SOLVESSOTM 200 adds to 100%, stirs to transparent homogeneous phase is obtained, and obtains 11% Picarbutrazox Fluoxastrobins breast Oil.
Embodiment 18：30% Picarbutrazox pyraclostrobin missible oils
Picarbutrazox 5%, pyraclostrobin 25%, ethoxylated castor oil 5%, calcium dodecyl benzene sulfonate 3%, SOLVESSOTM 200 adds to 100%, stirs to transparent homogeneous phase is obtained, and obtains 30% Picarbutrazox pyrazoles ether bacterium Emulsifiable concentrate.
Embodiment 19：20%Picarbutrazox Fluoxastrobin microcapsule suspension-suspendinagent agents
Picarbutrazox 10%, Fluoxastrobin 10%, Synperonic PE/64 15%, citric acid 0.05%, water 10%, PAPI 20%, SOLVESSOTM 100 5%, dispersant LFH0.15%, defoamer 0.16%, urea 5.5%；Water is complemented to 100%；In the oil phase aqueous solution of the addition containing Synperonic PE/64 that PAPI, Fluoxastrobin, SOLVESSOTM100 are formed, shape Into emulsion.Then heat and be incubated and add catalyst reaction 2 hours at 50 DEG C.The microcapsules of Fluoxastrobin is obtained after cooling. Synperonic PE/64, dispersant LFH, defoamer, urea, Picarbutrazox and water are mixed in proportion, and through sand Mill, is prepared into suspending agent.The Fluoxastrobin microcapsules that will be obtained is added in the suspending agent of Picarbutrazox, is uniformly mixing to obtain 20%Picarbutrazox Fluoxastrobin microcapsule suspension-suspendinagent agents.
The 35%Picarbutrazox Fluoxastrobin seed coat agents of embodiment 20
Picarbutrazox 30%, Fluoxastrobin 5%, AEO sulfosuccinic acid monoesters disodium 10%, innovation wood Quality sulfoacid calcium 5%, xanthans 1%, bentonite 1%.Glycerine 5%, PVP-K30 1%, complements to 100%, and above-mentioned each component is pressed Ratio is well mixed, and through being sanded, is prepared into 35%Picarbutrazox Fluoxastrobin seed coat agents.
The 50%Picarbutrazox Fluoxastrobin coated granules of embodiment 21
Picarbutrazox 10%, Fluoxastrobin 40%, polyethylene glycol 3%, the silicic acid 1% of high degree of dispersion, calcium carbonate complements to 100%, In a mixer, by levigate active component even spread to the carrier soaked by polyethylene glycol, 50% can be obtained in this way The dust-free coated granules agent of Picarbutrazox Fluoxastrobins.
Be well mixed for Picarbutrazox 50% and Fluoxastrobin 50% by embodiment 22.
Be well mixed for Picarbutrazox 50% and pyraclostrobin 50% by embodiment 23.
Above example proportioning is weight proportion.
Biological test example：
The combination of active principles of different agricultural chemicals is made agricultural chemicals, is exploitation at present and development novel pesticide and preventing and treating agriculturally resistance A kind of effective and quick way of germ.After the pestsides synthesis of different cultivars, three kinds of type of action are typically exhibited：It is added and makees With, synergistic effect and antagonism.But which kind of effect be specially, it is impossible to predict, only just be can know that by many experiments.Compounding Synergy is formulated well, due to significantly improving actual prevention effect, reduces the usage amount of agricultural chemicals, so as to greatly delay The generation of resistance.
First, virulence test：
The toxicity index of each medicament and the co-toxicity coefficient of mixture are calculated according to the abundant methods of Sun Yun（CTC values）, when CTC≤80, then combine Thing shows antagonism, when 80<CTC<120, then composition show summation action, when CTC >=120, then composition performance Go out synergistic effect.
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
B in the percentage composition+B medicament toxicity index * mixtures of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures Percentage composition
Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixture theoretical toxicity index (TTI) * 100
Experiment 1：The toxicity test of cucumber downy mildew
Selected from the cucumber seedling that growing way is consistent, sprayed under 50PSI pressure with potter spray towers, per basin about 5mL, each medicine Agent sets 12 concentration gradients.24h connects bacterium after chemicals treatment, and the cucumber downy mildew leaf that will pick up from field is uniform above cucumber seedling Shake off conidium to be inoculated with, then cucumber seedling is put into greenhouse and is cultivated.Morbidity after 7d according to cucumber downy mildew is classified Standard complete stool investigates disease index, and calculates prevention effect, and concentration EC50 in suppressing then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).
Table 1
As known from Table 1, Picarbutrazox and Fluoxastrobin or pyraclostrobin are preventing the weight proportion 50 of cucumber downy mildew: 1-1:When in 50 scope, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect Really.
Experiment 2：The toxicity test of tomato blight
Selected from the tomato seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the tomato in field Epidemic disease leaf is uniformly shaken off conidium above tomato seedling and is inoculated with, and then tomato seedling is put into greenhouse and is cultivated.Pressed after 7d Morbidity grade scale complete stool according to tomato blight investigates disease index, and calculates prevention effect, is then calculated with least square method Concentration EC50 in suppression, then calculate co-toxicity coefficient (CTC) according to the abundant methods of Sun Yun.
Table 2：Virulence test result of the present invention on preventing and treating tomato blight
As known from Table 2, Picarbutrazox and Fluoxastrobin or pyraclostrobin are preventing the weight proportion 50 of tomato blight:1- 1:When in 50 scope, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect.
Experiment 3：The toxicity test of Penicillium italicum
Using suppression mycelial growth rate method：Experiment target is designated as Penicillium italicum.
Picarbutrazox and Fluoxastrobin or pyraclostrobin are used into acetone solution respectively, then with 0.1% Tween-80 Aqueous solution dilution is configured to the liquid of series concentration, draws 6mL to the conical flask of sterilizing respectively in superclean bench, adds 50 DEG C or so of potato dextrose agar（PDA）54mL, pours into 4 plates of diameter 9cm after shaking up, be made 4 The toxic culture medium of respective concentration；With same method by the Picarbutrazox and Fluoxastrobin of different ratio or pyrazoles ether bacterium Ester series concentration compound solution is made toxic culture medium.The Penicillium italicum bacterium of 2 days will be cultivated, existed with the card punch of diameter 5mm Colony edge breaks into bacterium block, and bacterium block is moved to the toxic PDA culture medium center being configured in advance with transfer needle, is subsequently placed in 25 DEG C Culture in incubator, often treatment is repeated 4 times.After 3 days, each treatment colony diameter cm is measured using crossing method slide calliper rule, asked Go out correction and suppress percentage.Two diameters are surveyed in each bacterium colony right-angled intersection, and bacterium colony size is represented with its average.Then as the following formula Obtain colony growth inhibiting rate：
Then concentration EC in suppressing is calculated with least square method50, then calculate co-toxicity coefficient according to the abundant methods of Sun Yun（CTC）.
Table 3：To the virulence test result of Penicillium italicum
As known from Table 3, the combination preventing and treating Penicillium italicum of Picarbutrazox and Fluoxastrobin or pyraclostrobin is in proportioning 50: 1-1:When in 50 scope, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect Really.
Experiment 4：The toxicity test of rice seedling blight
Selected from the rice seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers in 50PSI pressure Lower spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the water in field Rice damping-off leaf is uniformly shaken off conidium above rice seedling and is inoculated with, and then rice seedling is put into greenhouse and is trained Support.Morbidity grade scale complete stool after 7d according to rice seedling blight investigates disease index, and calculates prevention effect, then with minimum Square law calculates concentration EC50 in suppressing, then calculates co-toxicity coefficient (CTC) according to the abundant methods of Sun Yun.
Table 4：Virulence test result of the present invention on preventing and treating rice seedling blight
As known from Table 4, Picarbutrazox and Fluoxastrobin or pyraclostrobin are preventing the weight proportion 50 of rice seedling blight: 1-1:When in 50 scope, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect Really.
Test 5 powdery mildews（Wheat）Toxicity test
Selected from the wheat seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the wheat in field Powdery mildew leaf is uniformly shaken off conidium above wheat seedling and is inoculated with, and then wheat seedling is put into greenhouse and is cultivated.After 7d Morbidity grade scale complete stool according to wheat powdery mildew investigates disease index, and calculates prevention effect, then uses least square method Concentration EC50 in suppressing is calculated, then co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.
Table 5：Virulence test result of the present invention on preventing and treating wheat powdery mildew
As known from Table 5, Picarbutrazox and Fluoxastrobin or pyraclostrobin are preventing the weight proportion 50 of wheat powdery mildew: 1-1:When in 50 scope, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect Really.
Test 6 leaf blights（Wheat）Toxicity test
Selected from the wheat seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the wheat in field Leaf spoting bacteria is uniformly shaken off conidium above wheat seedling and is inoculated with, and then wheat seedling is put into greenhouse and is cultivated.After 7d Morbidity grade scale complete stool according to wheat leaf blight investigates disease index, and calculates prevention effect, then uses least square method Concentration EC50 in suppressing is calculated, then co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.
Table 6：Virulence test result of the present invention on preventing and treating wheat leaf blight
As known from Table 6, Picarbutrazox and Fluoxastrobin or pyraclostrobin are preventing the weight proportion 50 of wheat leaf blight: 1-1:When in 50 scope, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect Really.
2nd, the evaluation of pesticide effectiveness：
When the effect of active compound combinations exceedes the summation of the effect when each reactive compound is administered alone, there is association Same synergistic effect.The predictive role of two kinds of particular combinations of reactive compound can be used so-called " Colby formula " (referring to S.R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations ", Weeds 1967,15,20-22) it is calculated as below:If
X is the activity when the reactive compound A that consumption is mg/ha or concentration is mppm is used；
Y is the activity when the reactive compound B that consumption is ng/ha or concentration is nppm is used, and is expressed as accounting for untreated right According to percentage；
E is the activity when the reactive compound A and B that consumption is m and n g/ha or concentration is m and n ppm is used,
So
If the activity of actual observation（O）More than expection activity（E）, then said composition has synergistic effect.
Following biological test example is used to illustrate the present invention.But, the present invention is not limited to these embodiments.
Experiment 7：Epidemic disease is tested（Tomato）The efficacy testing of/protectiveness
Solvent：24.5 pbw acetones
24.5 weight portion dimethylacetylamides
Emulsifying agent：1 weight portion alkylaryl polyglycol ether
In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.
In order to test protectiveness activity, young plant is sprayed with certain rate of application with active agent preparations.In sprinkling Coating dry after, plant is inoculated with the aqueous spore suspension of phytophthora infestans.Then the plant is placed on about 20 DEG C, in the incubate box that relative atmospheric humidity is 100%.
3 days after incubation, test result is evaluated.0% represents the drug effect equivalent to control sample, 100% drug effect Indicate and occur without illness.
Reactive compound Reactive compound is applied With rate, ppm Actual medicine Effect, % " Colby formula " is calculated Desired value %
Picarbutrazox 1 44 -
Picarbutrazox 10 53.4 -
Picarbutrazox 50 69.4 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 1 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 10 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 50 5 -
Fluoxastrobin 1 0 -
Fluoxastrobin 10 15 -
Fluoxastrobin 25 32 -
Fluoxastrobin 50 57.2 -
Fluoxastrobin 100 78.2 -
Fluoxastrobin 1000 89.3 -
Pyraclostrobin 1 0 -
Pyraclostrobin 10 18 -
Pyraclostrobin 25 38.4 -
Pyraclostrobin 50 63.7 -
Pyraclostrobin 100 83.6 -
Pyraclostrobin 1000 93.2 -
Picarbutrazox+ Fluoxastrobins 50+1 83.5 69.4
Picarbutrazox+ Fluoxastrobins 10+1 75.3 53.4
Picarbutrazox+ Fluoxastrobins 10+10 79.4 60.4
Picarbutrazox+ Fluoxastrobins 1+25 74.1 61.9
Picarbutrazox+Fluoxastrobin 1+50 85.2 76.0
Picarbutrazox+Fluoxastrobin 1+100 85.9 87.7
Picarbutrazox+Fluoxastrobin 1+1000 92.7 94.0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 50+1 5 5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+10 15 15
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+25 32 32
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+50 55.7 57.2
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+100 77.9 78.2
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+1000 89.1 89.3
Picarbutrazox+ pyraclostrobins 50+1 86.9 69.4
Picarbutrazox+ pyraclostrobins 10+1 81.4 53.4
Picarbutrazox+ pyraclostrobins 10+10 83.7 61.7
Picarbutrazox+ pyraclostrobins 1+25 80.5 65.5
Picarbutrazox+pyraclostrobin 1+50 89.4 79.6
Picarbutrazox+pyraclostrobin 1+100 90.5 90.8
Picarbutrazox+pyraclostrobin 1+1000 95.2 96.2
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 50+1 5 5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+10 18 18
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+25 37.5 38.4
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+50 65.3 63.7
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+100 83.5 83.6
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+1000 93.1 93.2
Upper table is clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyrazoles ether bacterium The weight ratio of ester is 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of tomato blight in the range of 50, that is, exists bright Aobvious cooperative effect.
Experiment 8：Epidemic disease is tested（Tomato）/ curative efficacy testing
Solvent：24.5 pbw acetones
24.5 weight portion dimethylacetylamides
Emulsifying agent：1 weight portion alkylaryl polyglycol ether
In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.
In order to test therapeutical active, the spore suspension of phytophthora infestans is inoculated with to the tomato of 5-6 leaf phases.Then will be described Prevented and treated 18 hours in the incubate box that plant is placed on about 20 DEG C, relative atmospheric humidity is 100%.After air-drying leaf, with activation Compound preparation sprays young plant with certain rate of application, and is sprayed with the degree of the liquid that drips, and then makes in greenhouse Its morbidity.Inoculation investigates occurring degree after 7 days.Test result is evaluated.0% represents the drug effect equivalent to control sample, 100% drug effect indicates occur without illness.
Reactive compound Reactive compound is applied With rate, ppm Actual medicine Effect, % " Colby formula " is calculated Desired value %
Picarbutrazox 1 44 -
Picarbutrazox 10 50.1 -
Picarbutrazox 50 62.1 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 1 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 10 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 50 5 -
Fluoxastrobin 1 0 -
Fluoxastrobin 10 11 -
Fluoxastrobin 25 27 -
Fluoxastrobin 50 51.5 -
Fluoxastrobin 100 71.3 -
Fluoxastrobin 1000 81.6 -
Pyraclostrobin 1 0 -
Pyraclostrobin 10 13 -
Pyraclostrobin 25 32.4 -
Pyraclostrobin 50 61.7 -
Pyraclostrobin 100 78.6 -
Pyraclostrobin 1000 90.2 -
Picarbutrazox+ Fluoxastrobins 50+1 77.8 62.1
Picarbutrazox+ Fluoxastrobins 10+1 73.6 50.1
Picarbutrazox+ Fluoxastrobins 10+10 72.6 55.6
Picarbutrazox+ Fluoxastrobins 1+25 71.3 59.1
Picarbutrazox+Fluoxastrobin 1+50 82.1 72.8
Picarbutrazox+Fluoxastrobin 1+100 83.2 83.9
Picarbutrazox+Fluoxastrobin 1+1000 88.6 89.7
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 50+1 5 5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+10 10 11
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+25 27 27
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+50 50.5 51.5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+100 71.1 71.3
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+1000 81.5 81.6
Picarbutrazox+ pyraclostrobins 50+1 80.1 62.1
Picarbutrazox+ pyraclostrobins 10+1 76.5 50.1
Picarbutrazox+ pyraclostrobins 10+10 78.4 50.1
Picarbutrazox+ pyraclostrobins 1+25 77.4 62.1
Picarbutrazox+pyraclostrobin 1+50 88.4 78.5
Picarbutrazox+pyraclostrobin 1+100 87.4 88.0
Picarbutrazox+pyraclostrobin 1+1000 93.1 94.5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 50+1 5 5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+10 14 13
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+25 31.7 32.4
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+50 62.8 61.7
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+100 78.5 78.6
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+1000 90.5 90.2
Upper table clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyraclostrobin Weight ratio is 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of tomato blight, i.e., in the presence of obvious in the range of 50 Cooperative effect.
Experiment 9：Downy mildew is tested（Grape）The efficacy testing of/protectiveness
Solvent：24.5 pbw acetones
24.5 weight portion dimethylacetylamides
Emulsifying agent：1 weight portion alkylaryl polyglycol ether
In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.
In order to test protectiveness activity, young plant is sprayed with certain rate of application with active agent preparations.In sprinkling Coating dry after, plant is inoculated with the aqueous spore suspension of Plasmopara viticola.Then the plant is placed on About 20 DEG C, in the incubate box that relative atmospheric humidity is 100%.
3 days after incubation, test result is evaluated.0% represents the drug effect equivalent to control sample, 100% drug effect Indicate and occur without illness.
Reactive compound Reactive compound is applied With rate, ppm Actual medicine Effect, % " Colby formula " is calculated Desired value %
Picarbutrazox 1 26.9 -
Picarbutrazox 10 54.2 -
Picarbutrazox 50 92.6 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 1 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 10 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 50 0 -
Fluoxastrobin 1 0 -
Fluoxastrobin 10 5 -
Fluoxastrobin 25 17 -
Fluoxastrobin 50 43 -
Fluoxastrobin 100 67 -
Fluoxastrobin 1000 79 -
Pyraclostrobin 1 0 -
Pyraclostrobin 10 8 -
Pyraclostrobin 25 28 -
Pyraclostrobin 50 58 -
Pyraclostrobin 100 76 -
Pyraclostrobin 1000 84 -
Picarbutrazox+ Fluoxastrobins 50+1 100 92.6
Picarbutrazox+ Fluoxastrobins 10+1 76.2 54.2
Picarbutrazox+ Fluoxastrobins 10+10 85.3 56.5
Picarbutrazox+ Fluoxastrobins 1+25 61.2 39.3
Picarbutrazox+Fluoxastrobin 1+50 73.1 58.3
Picarbutrazox+Fluoxastrobin 1+100 74.7 75.8
Picarbutrazox+Fluoxastrobin 1+1000 84.4 84.6
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+10 5 5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+25 15 17
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+50 43 43
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+100 67 67
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+1000 77 79
Picarbutrazox+ pyraclostrobins 50+1 100 92.6
Picarbutrazox+ pyraclostrobins 10+1 78.6 54.2
Picarbutrazox+ pyraclostrobins 10+10 87.3 57.8
Picarbutrazox+ pyraclostrobins 1+25 63.6 47.3
Picarbutrazox+pyraclostrobin 1+50 77.6 69.2
Picarbutrazox+pyraclostrobin 1+100 80.4 82.4
Picarbutrazox+pyraclostrobin 1+1000 84.3 88.3
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+10 8 8
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+25 26.1 28
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+50 57.6 58
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+100 74.1 76
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+1000 83.8 84
Upper table is clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyrazoles ether bacterium The weight ratio of ester is 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of downy mildew of garpe in the range of 50, that is, exists Cooperative effect, and the effect of cooperative effect is the most obvious.
Experiment 10：Downy mildew is tested（Grape）/ curative efficacy testing
Solvent：24.5 pbw acetones
24.5 weight portion dimethylacetylamides
Emulsifying agent：1 weight portion alkylaryl polyglycol ether
In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.
In order to test therapeutical active, the grape to the 5-6 leaf phases in seedling-growing container is inoculated with the spore suspension of downy mildew of garpe Liquid, places 18 hours in the incubate box that 25 DEG C, relative atmospheric humidity are 100%.After air-drying leaf, active agent preparations are used Plant is sprayed with certain rate of application, then it is fallen ill in greenhouse.Inoculation investigates occurring degree after 10 days.0% represents phase When in the drug effect of control sample, 100% drug effect indicates occur without illness.
Reactive compound Reactive compound is applied With rate, ppm Actual medicine Effect, % " Colby formula " is calculated Desired value %
Picarbutrazox 1 22.9 -
Picarbutrazox 10 48.2 -
Picarbutrazox 50 86.6 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 1 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 10 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 50 0 -
Fluoxastrobin 1 0 -
Fluoxastrobin 10 0 -
Fluoxastrobin 25 13 -
Fluoxastrobin 50 38 -
Fluoxastrobin 100 62 -
Fluoxastrobin 1000 73 -
Pyraclostrobin 1 0 -
Pyraclostrobin 10 5 -
Pyraclostrobin 25 22 -
Pyraclostrobin 50 54 -
Pyraclostrobin 100 71 -
Pyraclostrobin 1000 80 -
Picarbutrazox+ Fluoxastrobins 50+1 97.8 86.6
Picarbutrazox+ Fluoxastrobins 10+1 73.2 48.2
Picarbutrazox+ Fluoxastrobins 10+10 81.3 48.2
Picarbutrazox+ Fluoxastrobins 1+25 58.2 32.9
Picarbutrazox+Fluoxastrobin 1+50 71.1 52.2
Picarbutrazox+Fluoxastrobin 1+100 71.2.4 70.7
Picarbutrazox+Fluoxastrobin 1+1000 77.4 79.2
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+10 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+25 11 13
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+50 35 38
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+100 61 62
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+1000 73 73
Picarbutrazox+ pyraclostrobins 50+1 98.3 86.6
Picarbutrazox+ pyraclostrobins 10+1 75.2 48.2
Picarbutrazox+ pyraclostrobins 10+10 83.3 50.8
Picarbutrazox+ pyraclostrobins 1+25 61.2 39.8
Picarbutrazox+pyraclostrobin 1+50 75.1 64.6
Picarbutrazox+pyraclostrobin 1+100 76.1 77.6
Picarbutrazox+pyraclostrobin 1+1000 83.1 84.6
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+10 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+25 22 22
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+50 53 54
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+100 70 71
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+1000 77 80
Upper table is clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyrazoles ether bacterium The mass ratio of ester is 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of downy mildew of garpe in the range of 50, that is, exists Cooperative effect, and the effect of cooperative effect is the most obvious.
Experiment 11：Antiseptic effect to preserving oranges and tangerines
Treatment oranges and tangerines picking time plucks fruit, rejects disease fruit and wound fruit, and fruit 1min is soaked with treatment liquid.Control clear water dipping.Respectively Dried after treatment leaching fruit, next day loads the plastic box that surrounding is lined with newspaper, normal temperature laboratory storage.Check decayed fruit number within 7 days after treatment And decayed fruit is rejected, while having weighed up fruit weight.And calculate preventive effect.
Upper table is clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyrazoles ether bacterium The weight ratio of ester is 50:1-1:In the range of 50, to the anti-corrosion of storage period oranges and tangerines, actual drug effect is higher than the drug effect being calculated, There is obvious cooperative effect.
Experiment 12：Damping-off is tested（Paddy rice）/ curative efficacy testing
Solvent：24.5 pbw acetones
24.5 weight portion dimethylacetylamides
Emulsifying agent：1 weight portion alkylaryl polyglycol ether
In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.
In order to test therapeutical active, to the spore of the rice seedlings Inoculated Rice rhizoctonia solani of the 5-6 leaf phases in seedling-growing container Water slurry, places 18 hours in the incubate box that 25 DEG C, relative atmospheric humidity are 100%.After air-drying leaf, active ingredient is used Thing preparation sprays plant with certain rate of application, then it is fallen ill in greenhouse.Inoculation investigates occurring degree after 10 days.0% The drug effect equivalent to control sample is represented, 100% drug effect indicates occur without illness.
Reactive compound Reactive compound is applied With rate, ppm Actual medicine Effect, % " Colby formula " is calculated Desired value %
Picarbutrazox 1 25.9 -
Picarbutrazox 10 51.2 -
Picarbutrazox 50 89.8 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 1 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 10 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 50 0 -
Fluoxastrobin 1 0 -
Fluoxastrobin 10 0 -
Fluoxastrobin 25 10 -
Fluoxastrobin 50 37.8 -
Fluoxastrobin 100 67.4 -
Fluoxastrobin 1000 82.1 -
Pyraclostrobin 1 0 -
Pyraclostrobin 10 13 -
Pyraclostrobin 25 15 -
Pyraclostrobin 50 39.3 -
Pyraclostrobin 100 69.2 -
Pyraclostrobin 1000 85.8 -
Picarbutrazox+ Fluoxastrobins 50+1 100 89.8
Picarbutrazox+ Fluoxastrobins 10+1 81.4 51.2
Picarbutrazox+ Fluoxastrobins 10+10 86.4 51.2
Picarbutrazox+ Fluoxastrobins 1+25 56.8 33.3
Picarbutrazox+Fluoxastrobin 1+50 69.5 53.9
Picarbutrazox+Fluoxastrobin 1+100 73.8 74.8
Picarbutrazox+Fluoxastrobin 1+1000 83.8 86.7
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+10 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+25 9 10
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+50 34.1 37.8
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+100 66.4 67.4
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+1000 80.4 82.1
Picarbutrazox+ pyraclostrobins 50+1 100 89.8
Picarbutrazox+ pyraclostrobins 10+1 78.4 51.2
Picarbutrazox+ pyraclostrobins 10+10 80.4 57.5
Picarbutrazox+ pyraclostrobins 1+25 54.8 37.0
Picarbutrazox+pyraclostrobin 1+50 67.5 55.0
Picarbutrazox+pyraclostrobin 1+100 72.7 77.2
Picarbutrazox+pyraclostrobin 1+1000 84.7 89.5
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+10 11 13
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+25 15 15
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+50 33.7 39.3
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+100 68.7 69.2
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+1000 85.1 85.8
Upper table is clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyrazoles ether bacterium The weight ratio of ester is 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of rice seedling blight in the range of 50, that is, exists Cooperative effect, and the effect of cooperative effect is the most obvious.
Experiment 13：Powdery mildew is tested（Wheat）/ curative efficacy testing
Solvent：24.5 pbw acetones
24.5 weight portion dimethylacetylamides
Emulsifying agent：1 weight portion alkylaryl polyglycol ether
In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.
In order to test therapeutical active, the wheat seedling to the 5-6 leaf phases in seedling-growing container is inoculated with the spore of wheat powdery mildew Water slurry, places 18 hours in the incubate box that 25 DEG C, relative atmospheric humidity are 100%.After air-drying leaf, active ingredient is used Thing preparation sprays plant with certain rate of application, then it is fallen ill in greenhouse.Inoculation investigates occurring degree after 10 days.0% The drug effect equivalent to control sample is represented, 100% drug effect indicates occur without illness.
Reactive compound Reactive compound is applied With rate, ppm Actual medicine Effect, % " Colby formula " is calculated Desired value %
Picarbutrazox 1 21.4 -
Picarbutrazox 10 36.8 -
Picarbutrazox 50 58.6 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 1 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 10 0 -
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate 50 0 -
Fluoxastrobin 1 0 -
Fluoxastrobin 10 11 -
Fluoxastrobin 25 26 -
Fluoxastrobin 50 54.2 -
Fluoxastrobin 100 78.4 -
Fluoxastrobin 1000 87.2 -
Pyraclostrobin 1 0 -
Pyraclostrobin 10 18 -
Pyraclostrobin 25 32.8 -
Pyraclostrobin 50 59.1 -
Pyraclostrobin 100 83.1 -
Pyraclostrobin 1000 90.4 -
Picarbutrazox+ Fluoxastrobins 50+1 76.2 58.6
Picarbutrazox+ Fluoxastrobins 10+1 62.3 36.8
Picarbutrazox+ Fluoxastrobins 10+10 72.9 43.7
Picarbutrazox+ Fluoxastrobins 1+25 77.4 41.8
Picarbutrazox+Fluoxastrobin 1+50 82.1 64.0
Picarbutrazox+Fluoxastrobin 1+100 82.8 83.0
Picarbutrazox+Fluoxastrobin 1+1000 85.7 89.9
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 10+10 11 11
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+25 23 26
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+50 50.2 54.2
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+100 77.1 78.4
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+Fluoxastrobin 1+1000 85.2 87.2
Picarbutrazox+ pyraclostrobins 50+1 74.1 58.6
Picarbutrazox+ pyraclostrobins 10+1 65.3 36.8
Picarbutrazox+ pyraclostrobins 10+10 75.9 43.7
Picarbutrazox+ pyraclostrobins 1+25 76.4 47.1
Picarbutrazox+pyraclostrobin 1+50 82.1 67.8
Picarbutrazox+pyraclostrobin 1+100 83.8 86.7
Picarbutrazox+pyraclostrobin 1+1000 91.3 92.4
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 50+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+1 0 0
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 10+10 18 18
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+25 32.1 32.8
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+50 58.7 59.1
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+100 80.3 83.1
[[6- [[[[(z)-(1- methyl isophthalic acid H- tetrazolium -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- Base] amyl carbamate+pyraclostrobin 1+1000 85.6 90.4
Upper table is clearly illustrated according to bactericidal composition of the invention, Picarbutrazox and Fluoxastrobin or pyrazoles ether bacterium The weight ratio of ester is 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of wheat powdery mildew in the range of 50, that is, exists Cooperative effect, and the effect of cooperative effect is the most obvious.

Claims (12)

1. a kind of bactericidal composition, it is characterised in that：The bactericidal composition includes active component Picarbutrazox and phonetic bacterium The weight ratio of ester or pyraclostrobin, Picarbutrazox and Fluoxastrobin or pyraclostrobin is 50:1-1:50, preferably 25: 1-1:25, more preferably 10:1-1:10, more preferably 5:1-1:5.
2. bactericidal composition according to claim 1, it is characterised in that：The Picarbutrazox and Fluoxastrobin or pyrrole Azoles kresoxim-methyl quality sum accounts for the 5%-90% of the Bactericidal composite amount of substance, more preferably more preferably 10%-80%, 20%-60%.
3. bactericidal composition according to claim 1, it is characterised in that：The formulation of the bactericidal composition be suspending agent, Seed coat agent, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, missible oil, microemulsion, water Emulsion, effervescent tablet, ultra low volume liquids, suspension emulsion.
4. bactericidal composition according to claim 1, it is characterised in that also comprising filler and/or surfactant.
5. it is a kind of prevent and treat plant pathogen method, it is characterised in that the bactericidal composition described in claim 1 is acted on into cause In germ and/or its environment, or plant, seed, soil, region, material or space.
6. method according to claim 5, it is characterised in that by the Picarbutrazox described in claim 1 and phonetic Bacterium ester or pyraclostrobin are administered simultaneously or apply respectively or sequential application.
7. the bactericidal composition described in claim 1 is used to prevent and treat the purposes of fungi and bacterium.
8. the bactericidal composition described in claim 1 is used to prevent and treat the purposes of fungi and bacterium in cereal, water fruits and vegetables.
9. the bactericidal composition described in claim 1 is used to protect the use of plant propagation material and the plant organ for then growing On the way.
10. caused a disease in the place preventing and treating soil or cultivation medium of preventing and treating needed for the bactericidal composition described in claim 1 is applied to Or saprophytic fungi and the purposes of bacterium.
Bactericidal composition described in 11. claims 1 is used to prevent and treat the purposes of fruit vegetables storing period disease.
The plant pathogenic fungi of a kind of 12. control plants, plant part, plant propagation material and the plant organ for then growing Method, it is characterised in that the method is included the bactericidal composition described in claim 1 with agronomy effectively and substantially without plant poison The amount of application of property is applied with Seed Treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution or smoke Etc. method be administered to plant, plant part, plant propagation material or plant growing or needing the soil that grows wherein or In cultivation medium.
CN201511008836.7A 2015-12-30 2015-12-30 A kind of bactericidal composition Pending CN106922694A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201511008836.7A CN106922694A (en) 2015-12-30 2015-12-30 A kind of bactericidal composition

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201511008836.7A CN106922694A (en) 2015-12-30 2015-12-30 A kind of bactericidal composition

Publications (1)

Publication Number Publication Date
CN106922694A true CN106922694A (en) 2017-07-07

Family

ID=59457593

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201511008836.7A Pending CN106922694A (en) 2015-12-30 2015-12-30 A kind of bactericidal composition

Country Status (1)

Country Link
CN (1) CN106922694A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107372532A (en) * 2017-08-11 2017-11-24 北京科发伟业农药技术中心 The bactericidal composition of the urethane of pyrrole containing tetrazolium
CN108184880A (en) * 2016-12-08 2018-06-22 深圳诺普信农化股份有限公司 Bactericidal composition containing picarbutrazox
CN110338009A (en) * 2019-07-11 2019-10-18 甘肃省农业工程技术研究院 A kind of control method of seed production corn base rot disease
CN111567175A (en) * 2020-05-07 2020-08-25 内蒙古农业大学 Method for preventing micro-organisms from skinning, intercepting, rainfall and infiltration in arid desert area
US20210227825A1 (en) * 2018-05-16 2021-07-29 Nippon Soda Co., Ltd. Safener

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101828560A (en) * 2010-06-02 2010-09-15 陕西上格之路生物科学有限公司 Agricultural sterilizing composite containing thifluzamide
CN106922710A (en) * 2015-12-29 2017-07-07 浙江新农化工股份有限公司 Compositions of the picarbutrazox with mitochondrial cytochrome enzyme inhibitor series bactericidal agent and its preparation and application

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101828560A (en) * 2010-06-02 2010-09-15 陕西上格之路生物科学有限公司 Agricultural sterilizing composite containing thifluzamide
CN106922710A (en) * 2015-12-29 2017-07-07 浙江新农化工股份有限公司 Compositions of the picarbutrazox with mitochondrial cytochrome enzyme inhibitor series bactericidal agent and its preparation and application

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨吉春等: "国内外杀菌剂进展", 《现代农药》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108184880A (en) * 2016-12-08 2018-06-22 深圳诺普信农化股份有限公司 Bactericidal composition containing picarbutrazox
CN107372532A (en) * 2017-08-11 2017-11-24 北京科发伟业农药技术中心 The bactericidal composition of the urethane of pyrrole containing tetrazolium
US20210227825A1 (en) * 2018-05-16 2021-07-29 Nippon Soda Co., Ltd. Safener
CN110338009A (en) * 2019-07-11 2019-10-18 甘肃省农业工程技术研究院 A kind of control method of seed production corn base rot disease
CN110338009B (en) * 2019-07-11 2021-04-16 甘肃省农业工程技术研究院 Method for preventing and treating basal rot of seed corn
CN111567175A (en) * 2020-05-07 2020-08-25 内蒙古农业大学 Method for preventing micro-organisms from skinning, intercepting, rainfall and infiltration in arid desert area
CN111567175B (en) * 2020-05-07 2022-04-08 内蒙古农业大学 Method for preventing micro-organisms from skinning, intercepting, rainfall and infiltration in arid desert area

Similar Documents

Publication Publication Date Title
CN106922694A (en) A kind of bactericidal composition
CN103988845B (en) A kind of Fungicidai mixtures
CN106922703A (en) A kind of bactericidal composition
CN106922706A (en) A kind of bactericidal composition
CN106922695A (en) A kind of bactericidal composition
CN106922697A (en) A kind of bactericidal composition
CN106922693A (en) A kind of bactericidal composition
CN106922720A (en) A kind of bactericidal composition
CN106922698A (en) A kind of bactericidal composition
CN106922721A (en) A kind of bactericidal composition
CN110384109A (en) A kind of bactericidal composition
CN110384104A (en) A kind of bactericidal composition
CN106922722A (en) A kind of bactericidal composition
CN106922704A (en) A kind of bactericidal composition
CN103988843B (en) A kind of bactericidal composition
CN103999870B (en) A kind of bactericidal composition
CN106922702A (en) A kind of bactericidal composition
CN106922705A (en) A kind of bactericidal composition
CN106922696A (en) A kind of bactericidal composition
CN106922699A (en) A kind of bactericidal composition
CN107318852A (en) A kind of bactericidal composition
CN106922701A (en) A kind of bactericidal composition
CN106922712A (en) A kind of bactericidal composition
CN106922700A (en) A kind of bactericidal composition
CN103988846B (en) A kind of bactericidal composition

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20170707