CN106872456A - Nitrite detection lateral chromatography test paper and preparation method thereof - Google Patents
Nitrite detection lateral chromatography test paper and preparation method thereof Download PDFInfo
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- CN106872456A CN106872456A CN201710078534.XA CN201710078534A CN106872456A CN 106872456 A CN106872456 A CN 106872456A CN 201710078534 A CN201710078534 A CN 201710078534A CN 106872456 A CN106872456 A CN 106872456A
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- 238000012360 testing method Methods 0.000 title claims abstract description 144
- 238000001514 detection method Methods 0.000 title claims abstract description 125
- 238000002360 preparation method Methods 0.000 title claims abstract description 43
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 title claims abstract description 32
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 64
- 239000002253 acid Substances 0.000 claims abstract description 53
- HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 claims abstract description 52
- 229950000244 sulfanilic acid Drugs 0.000 claims abstract description 52
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000243 solution Substances 0.000 claims description 39
- 239000003365 glass fiber Substances 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 14
- 238000007654 immersion Methods 0.000 claims description 12
- 238000011068 loading method Methods 0.000 claims description 8
- 229920002678 cellulose Polymers 0.000 claims description 6
- 239000001913 cellulose Substances 0.000 claims description 6
- 229920000728 polyester Polymers 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000012670 alkaline solution Substances 0.000 claims description 4
- ZAJAQTYSTDTMCU-UHFFFAOYSA-N 3-aminobenzenesulfonic acid Chemical compound NC1=CC=CC(S(O)(=O)=O)=C1 ZAJAQTYSTDTMCU-UHFFFAOYSA-N 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 239000000523 sample Substances 0.000 description 17
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000012535 impurity Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 4
- 150000001412 amines Chemical class 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- DMDOTRUOIVBPSF-UHFFFAOYSA-N naphthalene;hydrochloride Chemical compound Cl.C1=CC=CC2=CC=CC=C21 DMDOTRUOIVBPSF-UHFFFAOYSA-N 0.000 description 4
- 229910017604 nitric acid Inorganic materials 0.000 description 4
- 244000061458 Solanum melongena Species 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- VAYOSLLFUXYJDT-RDTXWAMCSA-N Lysergic acid diethylamide Chemical compound C1=CC(C=2[C@H](N(C)C[C@@H](C=2)C(=O)N(CC)CC)C2)=C3C2=CNC3=C1 VAYOSLLFUXYJDT-RDTXWAMCSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention provides a kind of nitrite detection lateral chromatography test paper, including it is successively set on detection reaction condition area in preparation, detection colour developing area and suction zones on base plate；One end of the detection reaction condition area in preparation is provided with well, and the detection reaction condition area in preparation presses in sample prescription to two test paper for being arranged in sequence with load acid solution and p-aminobenzene sulfonic acid, detection denuded zone is provided between two test paper；The detection colour developing area is the test paper for being loaded with hydrochloride naphthodiamide.Plurality of reagents cannot coexist in solving the problems, such as existing detection method, realize the detection paper of nitrite.
Description
Technical field
The invention belongs to technical field of chemical detection, and in particular to a kind of detection of nitrite with lateral chromatography test paper and its
Preparation method.
Background technology
Current chemical quick detection test paper, by all detection reagents, Test paper is made in fixation on a sheet of paper in advance,
By being got stuck structure assembling or with naked strips with circular hole after cutting, by be added dropwise or immersion sample extracting solution after, tried with detection
Agent reaction produces particular color, and the test paper color that detects by an unaided eye compares the content for judging detection material with standard color comparison card.
Existing chemical detection test paper is used for nitrite detection and problems with occurs：
1. detection reagent can bring background color per se with color after being fixed on test paper.
2. when the sample after extraction has band colored particle, the judgement of influence testing result after being added drop-wise on test paper.
3. after two kinds of reagent mixing immersions on a sheet of paper, stabilization is preserved in the basic conditions due to p-aminobenzene sulfonic acid,
It is apt to deteriorate in acid condition, and reaction condition is acidity, therefore the shelf-life of traditional detection test paper is not long.
4. step-reaction cannot be realized on a test paper, first step reaction is subject to the ambient influnences such as temperature, causes second step
Reaction has different testing results in same time.
5. naked bar picks extract solution, and color has loss to contaminated samples simultaneously, influences result judgement.
6. impurity removal function is difficult to realize on a test paper.
In sum, existing chemical detection test paper method is not used to the detection of nitrite.
The content of the invention
Based on above technical problem, the invention provides a kind of nitrite detection lateral chromatography test paper, solve existing
There are various incompatible problems of reaction reagent in detection method, realize the detection paper of nitrite.
Technical scheme is as follows：
A kind of nitrite detection lateral chromatography test paper, including the detection reaction condition being successively set on base plate prepares
Area, detection denuded zone, detection colour developing area and suction zones；Including the detection reaction condition area in preparation, the inspection that are successively set on base plate
Survey colour developing area and suction zones；One end of the detection reaction condition area in preparation is provided with well, and the detection reaction condition is accurate
Preparation area presses in sample prescription to two test paper for being arranged in sequence with load acid solution and p-aminobenzene sulfonic acid, and inspection is provided between two test paper
Survey denuded zone；The detection colour developing area is the test paper for being loaded with hydrochloride naphthodiamide.
Lateral chromatography test paper of the invention is free of NC films, and suction zones are blotting paper, and detection colour developing area is without detection line and matter
Control line.Because the flow velocity of NC films is slow, and can steep bad by acid-base solution, therefore cannot be used for detection colour developing area.
Detection sample first is added dropwise to well, detection sample reacts in area in preparation by liquid flow direction with reaction reagent,
After possessing color condition, finally reacted with developer in detection sample colour developing area, and according to chromatographic theory in the colour developing of colour developing area.This
The colour developing principle of test paper is：Nitrite under the mild acid conditions with p-aminobenzene sulfonic acid diazotising after, then with hydrochloride naphthodiamide
Coupling forms aubergine dyestuff.
Test paper used by detection denuded zone of the present invention and detection colour developing area is respectively qualitative filter paper, quantitative filter paper, suction
Water paper, glass fibre element film and polyester cellulose film in any one.
Preferably, described detection denuded zone is the glass fibre element film of blank.Glass fibre element film has filtering function,
The interference of little particle impurity can be well removed, while flow velocity is slower, is reacted fully；Preventing solution antedating response simultaneously influences
Course of reaction and reaction result.
Preferably, described detection colour developing area is the qualitative filter paper for being loaded with hydrochloride naphthodiamide, and qualitative filter paper is enough thin, together
When Tomography Velocity it is moderate, can guarantee that the reaction time is sufficient, distribution of color is uniform after colour developing.
Test paper used by detection reaction condition area in preparation of the present invention is respectively qualitative filter paper, quantitative filter paper, water suction
Paper, glass fibre element film and polyester cellulose film in any one or it is various.
Preferably, the test paper of the detection reaction condition area in preparation load p-aminobenzene sulfonic acid is blotting paper.Blotting paper
Preferably, the reaction reagent and sample solution that can guarantee that q.s react more abundant to water absorbing capacity.
Preferably, the test paper of the detection reaction condition area in preparation load acid solution is glass fibre element film.Due to amino
Benzene sulfonic acid test paper is in alkalescent, and detects reaction and reacted under mild acid conditions more fully, therefore in p-aminobenzene sulfonic acid test paper
It is preceding that acid test paper is set, for reaction provides acid condition；Meanwhile, glass fibre element film has filtering function, can remove before the reaction
Go the interference of sample small particles impurity.
The acid solution loaded in present invention detection reaction condition area in preparation is trichloroacetic acid.Trichloroacetic acid is solid strong acid, its
His hydrochloric acid, sulfuric acid and nitric acid all have volatility, can all be vapored away after immersion test paper drying, and sulfuric acid, and nitric acid has oxygen
The property changed, therefore the present invention has selected trichloroacetic acid.
Base plate of the present invention is PVC board.
Present invention also offers the nitrite detection preparation method of lateral chromatography test paper, comprise the following steps：
A, prepare that p-aminobenzene sulfonic acid test paper, acid test paper and hydrochloride naphthodiamide test paper are standby respectively, preparation method is such as
Under：
P-aminobenzene sulfonic acid test paper：Blotting paper is soaked in the alkaline solution of the p-aminobenzene sulfonic acid of 4g/L, 25- is soaked
40 minutes, it is placed in 30-40 DEG C of baking oven and dries completely, saves backup；
Acid test paper：Glass fibre element film is soaked in the trichloroacetic acid solution of 40g/L, after soaking 25-40 minutes
It is placed in 40-60 DEG C of baking oven and dries completely, saves backup；
Hydrochloride naphthodiamide test paper：Qualitative filter paper is soaked in the hydrochloride naphthodiamide aqueous solution of 4g/L, 25-40 is soaked
It is placed in 40-60 DEG C of baking oven after minute and is dried completely, is saved backup；
B, according to Loading sequence by acid test paper, blank glass fiber element film, p-aminobenzene sulfonic acid test paper, hydrochloric acid naphthalene second two
Amine test paper and blotting paper are assembled on base plate successively.
Preferably, the alkaline solution of the p-aminobenzene sulfonic acid refers to the hydrogen that p-aminobenzene sulfonic acid is dissolved in 0.03mol/L
Sodium hydroxide solution, makes the test paper shelf-life longer with weak basic condition protection p-aminobenzene sulfonic acid.
The beneficial effects of the present invention are：
1st, be separately fixed at chemical detection reagent on test paper by the lateral chromatography test paper, according to reaction sequence according to lateral layer
Analysis structure assembling, solves various incompatible problems of reaction reagent, nitrite elder generation in existing nitrite detection method
Under the mild acid conditions with p-aminobenzene sulfonic acid diazotising after, then be coupled to form aubergine dyestuff with hydrochloride naphthodiamide.Make nitrous
Hydrochlorate detection is gradually completing detection reaction on test paper, realizes detection paper.
2nd, due to p-aminobenzene sulfonic acid test paper, the holding time is most long under weak basic condition, and detects reaction in mild acid conditions
Carry out, therefore acid test paper is set before p-aminobenzene sulfonic acid test paper, for reaction provides acid condition.Meanwhile, select glass fibers
The plain film load acid solution of dimension, glass fibre element film has filtering function, can before the reaction remove the interference of sample small particles impurity；
Selection blotting paper load p-aminobenzene sulfonic acid, the water absorbing capacity of blotting paper preferably, can guarantee that the reaction reagent and sample of q.s
This solution reaction is more abundant, and sensitivity is high.
The 3rd, the glass fibre of blank is set between the sour water test paper and p-aminobenzene sulfonic acid test paper of reaction condition area in preparation
Plain film, glass fibre element film tool filtering function, can well remove the interference of little particle impurity, while flow velocity is slower, make reaction
Fully, preventing solution antedating response influences course of reaction and reaction result, is further ensured that the accuracy of reaction.
4th, the present invention selects trichloroacetic acid to provide acid condition for reaction, and trichloroacetic acid is solid strong acid, other hydrochloric acid, sulphur
Acid and nitric acid all have volatility, can all be vapored away after immersion test paper drying, and sulfuric acid, and nitric acid has oxidisability, therefore
The present invention has selected the trichloroacetic acid suitable for detection paper.
Brief description of the drawings
Fig. 1 is the structure chart of nitrite detection lateral chromatography test paper of the present invention.
Fig. 2 is the colour developing result of lateral chromatography detection paper nitrite various concentrations gradient.
Reference is：1st, base plate, 21, acid test paper, 22, p-aminobenzene sulfonic acid test paper, 3, detection denuded zone, 4, detection
Colour developing area, 5, suction zones.
Specific embodiment
Essentiality content of the invention is described in further detail with reference to specific embodiment.
Embodiment 1
As shown in figure 1, lateral chromatography test paper is used in a kind of nitrite detection, including it is successively set on the detection on base plate 1
Reaction condition area in preparation, detection colour developing area 4 and suction zones 5；One end of the detection reaction condition area in preparation is provided with well,
The detection reaction condition area in preparation presses in sample prescription to two test paper for being arranged in sequence with load acid solution and p-aminobenzene sulfonic acid, two
Detection denuded zone 3 is provided between test paper；The detection colour developing area 4 is the test paper for being loaded with hydrochloride naphthodiamide.
Two test paper of load acid solution and p-aminobenzene sulfonic acid are the acid test paper 21 and p-aminobenzene sulfonic acid test paper in Fig. 1
22。
Embodiment 2
As shown in figure 1, lateral chromatography test paper is used in a kind of nitrite detection, including it is successively set on the detection on base plate 1
Reaction condition area in preparation, detection colour developing area 4 and suction zones 5；One end of the detection reaction condition area in preparation is provided with well,
The detection reaction condition area in preparation presses in sample prescription to two test paper for being arranged in sequence with load acid solution and p-aminobenzene sulfonic acid, two
Detection denuded zone 3 is provided between test paper；The detection colour developing area 4 is the test paper for being loaded with hydrochloride naphthodiamide.
Two test paper of load acid solution and p-aminobenzene sulfonic acid are the acid test paper 21 and p-aminobenzene sulfonic acid test paper in Fig. 1
22。
Detection sample first is added dropwise to well, detection sample presses liquid flow direction in area in preparation and p-aminobenzene sulfonic acid diazotising
Afterwards, possess color condition, finally be coupled to form aubergine dyestuff with hydrochloride naphthodiamide in detection sample colour developing area, according to chromatography
Principle is in the colour developing of colour developing area.
The test paper of the load acid solution is blotting paper.
The test paper of the load p-aminobenzene sulfonic acid is quantitative filter paper.
Test paper used by the detection denuded zone 3 is polyester cellulose film.
Test paper used by the detection colour developing area 4 is quantitative filter paper.
Embodiment 3
The present embodiment is on the basis of embodiment 1：
The test paper of the load acid solution is polyester cellulose film.
The test paper of the load p-aminobenzene sulfonic acid is qualitative filter paper.
Test paper used by the detection denuded zone 3 is blotting paper.
Test paper used by the detection colour developing area 4 is glass fibre element film.
Embodiment 4
The present embodiment is on the basis of embodiment 1：
The test paper of the detection reaction condition area in preparation load p-aminobenzene sulfonic acid is blotting paper, and loading the test paper of acid solution is
Glass fibre element film.
Described detection denuded zone 3 is the glass fibre element film of blank.
Described detection colour developing area 4 is the quantitative filter paper for being loaded with hydrochloride naphthodiamide.
The acid solution loaded in the detection reaction condition area in preparation is trichloroacetic acid.
Embodiment 5
The present embodiment is on the basis of embodiment 1：
The test paper of the detection reaction condition area in preparation load p-aminobenzene sulfonic acid is blotting paper, and loading the test paper of acid solution is
Glass fibre element film.
Described detection denuded zone 3 is the glass fibre element film of blank.
Described detection colour developing area 4 is the qualitative filter paper for being loaded with hydrochloride naphthodiamide.
The acid solution loaded in the detection reaction condition area in preparation is trichloroacetic acid.
Embodiment 6
The nitrite detection preparation method of lateral chromatography test paper of embodiment 5, comprises the following steps：
A, prepare that p-aminobenzene sulfonic acid test paper, acid test paper and hydrochloride naphthodiamide test paper are standby respectively, preparation method is such as
Under：
P-aminobenzene sulfonic acid test paper：Blotting paper is soaked in 4g/L p-aminobenzene sulfonic acid 0.03mol/L sodium hydroxide solutions,
Immersion 30 minutes, is placed in 35 DEG C of baking ovens and dries completely, saves backup；
Acid test paper：Glass fibre element film is soaked in the trichloroacetic acid solution of 40g/L, immersion is placed in after 30 minutes
Dried completely in 50 DEG C of baking ovens, saved backup；
Hydrochloride naphthodiamide test paper：Qualitative filter paper is soaked in the hydrochloride naphthodiamide aqueous solution of 4g/L, 30 points are soaked
It is placed in after clock in 50 DEG C of baking ovens and is dried completely, is saved backup；
B, according to Loading sequence by acid test paper, blank glass fiber element film, p-aminobenzene sulfonic acid test paper, hydrochloric acid naphthalene second two
Amine test paper and blotting paper are assembled on base plate successively.
As shown in figure 1, acid test paper 21 and p-aminobenzene sulfonic acid test paper 22 are assembled in into detection reaction condition successively preparing
Area, hydrochloride naphthodiamide test paper is assembled in detection colour developing area 4, and detection denuded zone 3 is one layer of glass fibre element film of blank.
Embodiment 7
The nitrite detection preparation method of lateral chromatography test paper of embodiment 5, comprises the following steps：
A, prepare that p-aminobenzene sulfonic acid test paper, acid test paper and hydrochloride naphthodiamide test paper are standby respectively, preparation method is such as
Under：
P-aminobenzene sulfonic acid test paper：Blotting paper is soaked in 4g/L p-aminobenzene sulfonic acid 0.03mol/L sodium hydroxide solutions,
Immersion 25 minutes, is placed in 30 DEG C of baking ovens and dries completely, saves backup；
Acid test paper：Glass fibre element film is soaked in the trichloroacetic acid solution of 40g/L, immersion is placed in after 25 minutes
Dried completely in 40 DEG C of baking ovens, saved backup；
Hydrochloride naphthodiamide test paper：Qualitative filter paper is soaked in the hydrochloride naphthodiamide aqueous solution of 4g/L, 25 points are soaked
It is placed in after clock in 40 DEG C of baking ovens and is dried completely, is saved backup；
B, according to Loading sequence by acid test paper, blank glass fiber element film, p-aminobenzene sulfonic acid test paper, hydrochloric acid naphthalene second two
Amine test paper and blotting paper are assembled on base plate successively.
Embodiment 8
The nitrite detection preparation method of lateral chromatography test paper of embodiment 5, comprises the following steps：
A, prepare that p-aminobenzene sulfonic acid test paper, acid test paper and hydrochloride naphthodiamide test paper are standby respectively, preparation method is such as
Under：
P-aminobenzene sulfonic acid test paper：Blotting paper is soaked in 4g/L p-aminobenzene sulfonic acid 0.03mol/L sodium hydroxide solutions,
Immersion 40 minutes, is placed in 40 DEG C of baking ovens and dries completely, saves backup；
Acid test paper：Glass fibre element film is soaked in the trichloroacetic acid solution of 40g/L, immersion is placed in after 40 minutes
Dried completely in 60 DEG C of baking ovens, saved backup；
Hydrochloride naphthodiamide test paper：Qualitative filter paper is soaked in the hydrochloride naphthodiamide aqueous solution of 4g/L, 40 points are soaked
It is placed in after clock in 60 DEG C of baking ovens and is dried completely, is saved backup；
B, according to Loading sequence by acid test paper, blank glass fiber element film, p-aminobenzene sulfonic acid test paper, hydrochloric acid naphthalene second two
Amine test paper and blotting paper are assembled on base plate successively.
Ensure lucifuge operation as far as possible during solution and test paper is prepared, perishable coloured effect is anti-because reagent is shown in light
Answer result.
Due to p-aminobenzene sulfonic acid, the holding time is not long in acid solution and water, apt to deteriorate, and solubility is not high, and
Solubility is high and the holding time is more long under weak basic condition, therefore p-aminobenzene sulfonic acid solution is in alkalescent, in alkalescent bar
Preserved under part.During detection, acid test paper provides acid condition for detection reaction, after sample extracting solution is added dropwise, according to reaction sequence
Carry out obtaining predetermined detection effect.
Because detection reaction is two detection reactions, the first step detection no color of reaction is produced but generation second step shows
Colour response raw material, second step and first step product reaction generation color.So the first step extent of reaction is directly affected in phase
With detection reaction solution situation in the time.Lateral chromatography structure test paper of the invention stops time guarantee reaction to first step reaction
More fully, develop the color evenly.
Pattern detection
The μ g/ml of concentration gradient 5,10 μ g/ml, 20 μ g/ml, 30 μ g/ml, the 50 μ g/ml of nitrite solution are set, group is used
The test paper of dress detects to various concentrations respectively, obtains progressively progressive colour developing result as colorimetric reference, as shown in Figure 2；
With the detection paper sample to be tested of assembling, colour developing result and colorimetric reference is contrasted, you can in drawing sample
The concentration of nitrite.
The detection of test paper is limited to 5mg/kg.
Embodiment described above only expresses specific embodiment of the invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Shield scope.
Claims (9)
1. lateral chromatography test paper is used in a kind of nitrite detection, it is characterised in that the detection including being successively set on base plate is anti-
Answer condition area in preparation, detection colour developing area and suction zones；One end of the detection reaction condition area in preparation is provided with well, described
Detection reaction condition area in preparation presses in sample prescription to two test paper for being arranged in sequence with load acid solution and p-aminobenzene sulfonic acid, two examinations
Detection denuded zone is provided between paper；The detection colour developing area is the test paper for being loaded with hydrochloride naphthodiamide.
2. lateral chromatography test paper is used in nitrite detection according to claim 1, it is characterised in that the detection denuded zone
Qualitative filter paper, quantitative filter paper, blotting paper, glass fibre element film and polyester cellulose are respectively with the test paper used by detection colour developing area
Any one in film.
3. according to the nitrite detection lateral chromatography test paper described in claim 2, it is characterised in that described detection denuded zone
It is the glass fibre element film of blank.
4. according to the nitrite detection lateral chromatography test paper described in claim 2, it is characterised in that described detection colour developing area
To be loaded with the qualitative filter paper of hydrochloride naphthodiamide.
5. according to the nitrite detection lateral chromatography test paper described in claim 1, it is characterised in that the detection reaction condition
Test paper used by area in preparation is respectively in qualitative filter paper, quantitative filter paper, blotting paper, glass fibre element film and polyester cellulose film
Any one is various.
6. according to the nitrite detection lateral chromatography test paper described in claim 5, it is characterised in that the detection reaction condition
The test paper of area in preparation load p-aminobenzene sulfonic acid is blotting paper, and the test paper for loading acid solution is glass fibre element film.
7. lateral chromatography test paper is used in nitrite detection according to claim 1, it is characterised in that detection reaction condition is accurate
The acid solution loaded in preparation area is trichloroacetic acid.
8. the nitrite detection preparation method of lateral chromatography test paper, its feature according to claim 1-7 any one
It is to comprise the following steps：
A, prepare that p-aminobenzene sulfonic acid test paper, acid test paper and hydrochloride naphthodiamide test paper are standby respectively, preparation method is as follows：
P-aminobenzene sulfonic acid test paper：Blotting paper is soaked in the alkaline solution of the p-aminobenzene sulfonic acid of 4g/L, 25-40 points of immersion
Clock, is placed in 30-40 DEG C of baking oven and dries completely, saves backup；
Acid test paper：Glass fibre element film is soaked in the trichloroacetic acid solution of 40g/L, immersion is placed in after 25-40 minutes
Dried completely in 40-60 DEG C of baking oven, saved backup；
Hydrochloride naphthodiamide test paper：Qualitative filter paper is soaked in the hydrochloride naphthodiamide aqueous solution of 4g/L, is soaked 25-40 minutes
After be placed in 40-60 DEG C of baking oven and dry completely, save backup；
B, according to Loading sequence by acid test paper, blank glass fiber element film, p-aminobenzene sulfonic acid test paper, hydrochloride naphthodiamide try
Paper and blotting paper are assembled on base plate successively.
9. nitrite is detected with the preparation method of lateral chromatography test paper according to claim 8, it is characterised in that described right
The alkaline solution of aminobenzenesulfonic acid refers to the sodium hydroxide solution that p-aminobenzene sulfonic acid is dissolved in 0.03mol/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710078534.XA CN106872456A (en) | 2017-02-14 | 2017-02-14 | Nitrite detection lateral chromatography test paper and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710078534.XA CN106872456A (en) | 2017-02-14 | 2017-02-14 | Nitrite detection lateral chromatography test paper and preparation method thereof |
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| CN106872456A true CN106872456A (en) | 2017-06-20 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111208127A (en) * | 2020-01-21 | 2020-05-29 | 谱尼测试集团股份有限公司 | Nitrite rapid detection test paper |
| CN111208128A (en) * | 2020-01-21 | 2020-05-29 | 谱尼测试集团股份有限公司 | Test paper for rapidly detecting nitrite content |
| CN112129752A (en) * | 2020-09-25 | 2020-12-25 | 安徽理工大学 | Microfluidic nitrite concentration detection chip and detection method |
| CN112557381A (en) * | 2021-01-25 | 2021-03-26 | 河南省科学院生物研究所有限责任公司 | Detection test strip for alpha-galactosidase and detection method thereof |
| WO2023201671A1 (en) * | 2022-04-22 | 2023-10-26 | 宁德时代新能源科技股份有限公司 | Measurement assembly and manufacturing method therefor, and measurement apparatus and measurement system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1458514A (en) * | 2003-06-11 | 2003-11-26 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Nitrite detection test paper and its preparing method |
| CN101236163A (en) * | 2007-02-01 | 2008-08-06 | 张洪滔 | Method for detecting food product nitrite content |
| CN104535712A (en) * | 2014-12-26 | 2015-04-22 | 华南师范大学 | Test paper for rapidly testing nitrite and standard colourimetric card and application of test paper |
| CN104764738A (en) * | 2014-01-04 | 2015-07-08 | 无锡市申瑞生物制品有限公司 | Qualitative calcium test strip and manufacturing method thereof |
-
2017
- 2017-02-14 CN CN201710078534.XA patent/CN106872456A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1458514A (en) * | 2003-06-11 | 2003-11-26 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Nitrite detection test paper and its preparing method |
| CN101236163A (en) * | 2007-02-01 | 2008-08-06 | 张洪滔 | Method for detecting food product nitrite content |
| CN104764738A (en) * | 2014-01-04 | 2015-07-08 | 无锡市申瑞生物制品有限公司 | Qualitative calcium test strip and manufacturing method thereof |
| CN104535712A (en) * | 2014-12-26 | 2015-04-22 | 华南师范大学 | Test paper for rapidly testing nitrite and standard colourimetric card and application of test paper |
Non-Patent Citations (4)
| Title |
|---|
| LEUNG, WM 等: "One-step quantitative cortisol dipstick with proportional reading", 《JOURNAL OF IMMUNOLOGICAL METHODS》, vol. 281, no. 1, 1 October 2003 (2003-10-01), pages 109 - 118, XP004468183, DOI: 10.1016/j.jim.2003.07.009 * |
| 张改平 主编: "《免疫层析试纸快速检测技术》", vol. 1, 河南科学技术出版社, pages: 6 - 8 * |
| 贾宇？等: "食品中亚硝酸盐快速检测试纸条的研制", 《中国动物检疫》 * |
| 贾宇？等: "食品中亚硝酸盐快速检测试纸条的研制", 《中国动物检疫》, vol. 32, no. 04, 20 April 2015 (2015-04-20), pages 72 - 74 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111208127A (en) * | 2020-01-21 | 2020-05-29 | 谱尼测试集团股份有限公司 | Nitrite rapid detection test paper |
| CN111208128A (en) * | 2020-01-21 | 2020-05-29 | 谱尼测试集团股份有限公司 | Test paper for rapidly detecting nitrite content |
| CN112129752A (en) * | 2020-09-25 | 2020-12-25 | 安徽理工大学 | Microfluidic nitrite concentration detection chip and detection method |
| CN112557381A (en) * | 2021-01-25 | 2021-03-26 | 河南省科学院生物研究所有限责任公司 | Detection test strip for alpha-galactosidase and detection method thereof |
| WO2023201671A1 (en) * | 2022-04-22 | 2023-10-26 | 宁德时代新能源科技股份有限公司 | Measurement assembly and manufacturing method therefor, and measurement apparatus and measurement system |
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