CN110220896A - A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance - Google Patents
A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance Download PDFInfo
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- CN110220896A CN110220896A CN201910614716.3A CN201910614716A CN110220896A CN 110220896 A CN110220896 A CN 110220896A CN 201910614716 A CN201910614716 A CN 201910614716A CN 110220896 A CN110220896 A CN 110220896A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention provides a kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance, and reaction colorimetric card and a qualitative detection item after detection liquid is added when being equipped with the 5ml detection liquid centrifuge tube containing 3ml detection liquid, the blank centrifuge tube of 5ml, a different paraquat concentration in the kit.The poisonous substance qualitative method includes the following steps: that (1) sample to be tested adds in detection liquid, and the mass ratio of sample to be tested and detection liquid is 1:4 ~ 4:1；(2) it if the mixed liquor in step (1) changes colour, represents and contains paraquat in sample to be tested；(3) it is soaked into sample to be tested when using qualitative detection item immersion end, reaction tank changes colour immediately, represents and contains paraquat in sample to be tested.The principle of metachromasia occurs under alkaline condition using sodium dithionite and paraquat for the present invention, prepare qualitative detection item, qualitative and half-quantitative detection kit, paraquat poisoning can be excluded for clinic and new diagnostic method is provided, effectively serve in clinic, there is high social value.
Description
Technical field
The present invention relates to medical quick detection reagent, test paper to research and develop field, and in particular to a kind of poisonous substance is qualitative and sxemiquantitative
Detection kit and the poisonous substance qualitative method for utilizing it are applied to clinical quickly poisonous substance detection.
Background technique
The whole nation is only limitted to public security, pesticide medicament residue detection in agronomy for poisonous substance detection at present, medically only few
Number hospital can carry out poisonous substance detection, and all detect examination without the quick poisonous substance for poisonous substance in hospital in the whole country at present
Agent box, clinic poisoning judging efficiency are low.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of qualitative half-quantitative detection kits of poisonous substance and poisonous substance qualitative half
Quantitative approach prepares qualitative detection item, half-quantitative detection kit using simple metachromasia principle, can be clinical row
Except paraquat poisoning provides new diagnostic method, clinic is effectively served in, there is high social value.
In order to solve the above technical problems, the embodiment of the present invention provides a kind of qualitative half-quantitative detection kit of poisonous substance, institute
State the detection liquid centrifuge tube that a 5ml containing 3ml detection liquid is equipped in kit, the blank centrifuge tube of 5ml, one not
With the reaction colorimetric card and a qualitative detection reagent strip after addition detection liquid when paraquat concentration.
The present invention also provides a kind of preparation methods for detecting liquid, and in 5ml test tube, 3ml ddH is added2O is sequentially added
At least sodium dithionite of 0.05g is added in at least alkaline reagent of 0.1g, wherein alkaline reagent and sodium dithionite
Mass ratio is 2:1；It shakes up, saves at room temperature.
Preferably, the alkaline reagent is sodium bicarbonate.
The present invention also provides a kind of qualitative semi-quantitative methods of the poisonous substance using kit, include the following steps:
(1) sample to be tested adds in detection liquid, and the mass ratio of sample to be tested and detection liquid is 1:4 ~ 4:1；
(2) it if the mixed liquor in step (1) changes colour, represents and contains paraquat in sample to be tested；
(3) it is soaked into sample to be tested when using qualitative detection item immersion end, reaction tank changes colour immediately, represents sample to be tested
In contain paraquat.
Wherein, the sample to be tested is one of serum, blood plasma and urine.
Preferably, the mass ratio of the sample to be tested and detection liquid is 1:1.
The present invention also provides a kind of poisonous substance qualitative detection reagent strip, including a substrate, immersion is followed successively by the substrate
It is logical to be equipped with addition pool, capillary in the immersion end to the region of mark line and detection line for end, mark line, detection line, handheld terminal
Road, the reaction tank for being preset with alkaline reagent and sodium dithionite；The addition pool, reaction tank are connected to by capillary channel, institute
The sum for stating the alkaline reagent and sodium dithionite in reaction tank is corresponding with the quantity of reaction tank.When impregnate end be dipped into
After test sample sheet (urine, serum, blood plasma), sample to be tested enters addition pool, enters reaction tank by capillary channel afterwards, once reaction
Pond changes colour, and represents and contains paraquat in sample to be tested.
The advantageous effects of the above technical solutions of the present invention are as follows: the present invention is using sodium dithionite and paraquat in alkali
Property under the conditions of the principle of metachromasia occurs, prepare qualitative detection item, half-quantitative detection kit, hundred grass can be excluded to be clinical
Withered poisoning provides new diagnostic method, effectively serves in clinic, has high social value.
Detailed description of the invention
Reaction colorimetric card after detection liquid is added when Fig. 1 is a different paraquat concentration；
Fig. 2 is the structural schematic diagram of detection kit in the present invention；
Fig. 3 is the structural schematic diagram of toxicity qualitative detection reagent strip of the present invention；
Fig. 4 is the structural schematic diagram in the present invention at addition pool.
Description of symbols:
1, end is impregnated；2, mark line；3, detection line；4, handheld terminal；5, addition pool；6, capillary channel；7, reaction tank；8, liquid is detected
Centrifuge tube；9, blank centrifuge tube；10, colorimetric card is reacted；11, qualitative detection reagent strip.
Specific embodiment
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with specific implementation
Example is described in detail.
As shown in Figure 1, the embodiment of the present invention provides a kind of qualitative half-quantitative detection kit of poisonous substance, set in the kit
There are detection liquid centrifuge tube, the blank centrifuge tube of 5ml, a different paraquat concentration of a 5ml containing 3ml detection liquid
When be added detection liquid after reaction colorimetric card 10 and a qualitative detection reagent strip.
The embodiment of the present invention also provides a kind of preparation method for detecting liquid, and in 5ml test tube, 3ml ddH is added2O, then according to
The secondary alkaline reagent that 0.1g or more is added, is added the sodium dithionite of 0.05g or more, wherein alkaline reagent and even two sulfurous
The mass ratio of sour sodium is 2:1；It shakes up, it at room temperature can long-term preservation.
Preferably, the alkaline reagent is sodium bicarbonate.
The embodiment of the present invention also provides a kind of qualitative semi-quantitative method of the poisonous substance using above-mentioned kit, including walks as follows
It is rapid:
(1) it takes one of serum, blood plasma and urine as sample to be tested, sample to be tested is added in detection liquid, sample to be tested
Mass ratio with detection liquid is 1:4 ~ 4:1, optimum quality ratio 1:1；
(2) if the mixed liquor in step (1) changes colour, such as become faint yellow, light blue, blue or blue black color, represent to
Contain paraquat in test sample sheet；
(3) it is soaked into sample to be tested when using qualitative detection item immersion end, reaction tank changes colour immediately, represents sample to be tested
In contain paraquat.
Influence for urine background color: urine itself is in yellow, and depth difference is very big, in order to eliminate this influence,
It is too deep if there is color, 1:1 ddH can be used2Row detection again after O dilution.
When Fig. 1 show different paraquat concentration, reaction colorimetric card after this detection liquid is added dropwise, the depth of grey blackness in Fig. 1
Superficial shows the blue depth after detection.
In experimentation, concentrations are more than 1.5*10-5 g/ml.According to the reaction colorimetric card, we can also be right
The semi-quantitative assessment of sample to be tested progress paraquat concentration.Such case, for being clinically diagnosed as the patient of paraquat poisoning
Treat curative effect and prognosis evaluation clinical value with higher and meaning, we can for the patient of paraquat poisoning,
Using our kits, daily blood urine concentration evaluation is carried out after being admitted to hospital, and according to the color of blood urine sample metachromasia, is gradually become
It is light until colourless time and patient's related symptoms, sign and auxiliary examination are as a result, can be with comprehensive assessment patient curative effect and pre-
Situation afterwards.
The present invention also provides a kind of poisonous substance qualitative detection reagent strip as shown in Figure 3, including a substrate, on the substrate
It is followed successively by and impregnates end 1, mark line 2, detection line 3, handheld terminal 4, set in the immersion end 1 to the region of mark line 2 and detection line 3
There are addition pool 5, capillary channel 6, the reaction tank 7 for being preset with alkaline reagent and sodium dithionite；The addition pool 5, reaction tank 7
Be connected to by capillary channel 6, as shown in figure 4, the alkaline reagent and sodium dithionite in the reaction tank 7 sum with react
The quantity in pond is corresponding.After immersion end is dipped into sample to be tested (urine, serum, blood plasma), sample to be tested enters addition pool, after
Enter reaction tank by capillary channel, once reaction tank changes colour, represents and contain paraquat in sample to be tested.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, without departing from the principles of the present invention, it can also make several improvements and retouch, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (7)
1. a kind of qualitative half-quantitative detection kit of poisonous substance, which is characterized in that be equipped with one in the kit and detected containing 3ml
After detection liquid is added when the detection liquid centrifuge tube of the 5ml of liquid, the blank centrifuge tube of 5ml, a different paraquat concentration
React colorimetric card and a qualitative detection reagent strip.
2. a kind of preparation method for detecting liquid, which is characterized in that in 5ml test tube, 3ml ddH is added2O is sequentially added at least
At least sodium dithionite of 0.05g is added, wherein the quality of alkaline reagent and sodium dithionite in the alkaline reagent of 0.1g
Than for 2:1；It shakes up, saves at room temperature.
3. the preparation method of detection liquid according to claim 2, which is characterized in that the alkaline reagent is sodium bicarbonate.
4. a kind of qualitative semi-quantitative method of poisonous substance using kit described in claim 1, which is characterized in that including walking as follows
It is rapid:
(1) sample to be tested adds in detection liquid, and the mass ratio of sample to be tested and detection liquid is 1:4 ~ 4:1；
(2) it if the mixed liquor in step (1) changes colour, represents and contains paraquat in sample to be tested；
(3) it is soaked into sample to be tested when using qualitative detection item immersion end, reaction tank changes colour immediately, represents sample to be tested
In contain paraquat.
5. the qualitative semi-quantitative method of poisonous substance according to claim 4, which is characterized in that the sample to be tested is serum, blood
One of slurry and urine.
6. the qualitative semi-quantitative method of poisonous substance according to claim 4, which is characterized in that the sample to be tested and detection liquid
Mass ratio is 1:1.
7. a kind of poisonous substance qualitative detection reagent strip, which is characterized in that including a substrate, be followed successively by the substrate impregnate end,
Mark line, detection line, handheld terminal are equipped with addition pool, capillary channel, pre- in the immersion end to the region of mark line and detection line
Reaction tank equipped with alkaline reagent and sodium dithionite；The addition pool, reaction tank are connected to by capillary channel, the reaction
The sum of alkaline reagent and sodium dithionite in pond is corresponding with the quantity of reaction tank.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910614716.3A CN110220896A (en) | 2019-07-09 | 2019-07-09 | A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance |
| PCT/CN2020/080795 WO2021004091A1 (en) | 2019-07-09 | 2020-03-24 | Qualitative and semi-quantitative toxicant detection reagent kit and qualitative and semi-quantitative method for toxicant |
| ZA2020/06353A ZA202006353B (en) | 2019-07-09 | 2020-10-13 | Qualitative and semiquantitative detection kit for toxicants and qualitative and semiquantitative method for toxicants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910614716.3A CN110220896A (en) | 2019-07-09 | 2019-07-09 | A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110220896A true CN110220896A (en) | 2019-09-10 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910614716.3A Pending CN110220896A (en) | 2019-07-09 | 2019-07-09 | A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN110220896A (en) |
| WO (1) | WO2021004091A1 (en) |
| ZA (1) | ZA202006353B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021004091A1 (en) * | 2019-07-09 | 2021-01-14 | 南通大学附属医院 | Qualitative and semi-quantitative toxicant detection reagent kit and qualitative and semi-quantitative method for toxicant |
Citations (4)
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|---|---|---|---|---|
| CN101881735A (en) * | 2010-06-11 | 2010-11-10 | 赵永秀 | Reagent for qualitatively detecting paraquat and method for detecting paraquat in urine |
| CN202486140U (en) * | 2011-11-11 | 2012-10-10 | 北京勤邦生物技术有限公司 | Kit for detecting tetracyclines antibiotic |
| CN103792231A (en) * | 2014-01-24 | 2014-05-14 | 河南科技大学第一附属医院 | Medical qualitative paraquat monitoring test paper |
| CN105806840A (en) * | 2016-03-23 | 2016-07-27 | 江苏省海洋资源开发研究院(连云港) | Reagent kit and method for qualitatively and semi-quantitatively detecting paraquat in serum or urine |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103399007A (en) * | 2013-08-01 | 2013-11-20 | 王丹 | Kit and detecting method for qualitatively detecting paraquat in urine |
| CN203705346U (en) * | 2014-01-24 | 2014-07-09 | 河南科技大学第一附属医院 | Medical paraquat qualitative monitoring test paper |
| CN104607259A (en) * | 2015-01-22 | 2015-05-13 | 中国人民武装警察部队后勤学院附属医院 | Paraquat detection reagent tube |
| WO2017139843A1 (en) * | 2016-02-15 | 2017-08-24 | Red Garage Ventures Pty Ltd | Colorimetric hydration sensor systems & methods |
| US10330603B1 (en) * | 2016-04-08 | 2019-06-25 | Michael D. Callahan | Mass produced, low cost, portable test kit for the detection and identification of chemical and biological agents |
| WO2018017197A1 (en) * | 2016-07-18 | 2018-01-25 | Siemens Healthcare Diagnostics Inc. | Improved low sample volume urinalysis assay strip, analytical kits, and methods of use related thereto |
| CN207908376U (en) * | 2018-03-21 | 2018-09-25 | 施康培医疗科技（武汉）有限公司 | Iodine test strip in a kind of urine |
| CN110220896A (en) * | 2019-07-09 | 2019-09-10 | 南通大学附属医院 | A kind of qualitative half-quantitative detection kit of poisonous substance and the qualitative semi-quantitative method of poisonous substance |
-
2019
- 2019-07-09 CN CN201910614716.3A patent/CN110220896A/en active Pending
-
2020
- 2020-03-24 WO PCT/CN2020/080795 patent/WO2021004091A1/en active Application Filing
- 2020-10-13 ZA ZA2020/06353A patent/ZA202006353B/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101881735A (en) * | 2010-06-11 | 2010-11-10 | 赵永秀 | Reagent for qualitatively detecting paraquat and method for detecting paraquat in urine |
| CN202486140U (en) * | 2011-11-11 | 2012-10-10 | 北京勤邦生物技术有限公司 | Kit for detecting tetracyclines antibiotic |
| CN103792231A (en) * | 2014-01-24 | 2014-05-14 | 河南科技大学第一附属医院 | Medical qualitative paraquat monitoring test paper |
| CN105806840A (en) * | 2016-03-23 | 2016-07-27 | 江苏省海洋资源开发研究院(连云港) | Reagent kit and method for qualitatively and semi-quantitatively detecting paraquat in serum or urine |
Non-Patent Citations (1)
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| 陈珊珊 等: "百草枯检测方法的研究进展", 《农药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021004091A1 (en) * | 2019-07-09 | 2021-01-14 | 南通大学附属医院 | Qualitative and semi-quantitative toxicant detection reagent kit and qualitative and semi-quantitative method for toxicant |
Also Published As
| Publication number | Publication date |
|---|---|
| ZA202006353B (en) | 2021-07-28 |
| WO2021004091A1 (en) | 2021-01-14 |
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Application publication date: 20190910 |