CN106852939A - Treat lactic bacteria composition of vaginitis and preparation method thereof - Google Patents
Treat lactic bacteria composition of vaginitis and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
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Abstract
The invention discloses a kind of lactic bacteria composition for treating vaginitis, including lactic acid bacteria combination core, the lactic acid bacteria combination core is wrapped with coated layer;The coated layer includes the composition of parts by weight:Oligoisomaltose 30~50, protein substance 30~50, Cranberry fruit powder 5~10, nitrocellulose 5~10;The lactic acid bacteria combination core is made up of the bacterial strain of following parts by weight:Lactobacillus salivarius 30~40, lactobacillus reuteri 20~30, Lactobacillus rhamnosus 10~20, lactobacterium acidophilus 10~20.The invention also discloses its preparation method.The present invention has the advantages that significantly to treat vaginitis and without any side effects.
Description
Technical field
The present invention relates to probiotics, with significantly treatment vaginitis and without any side effects controlled more particularly, to a kind of
Treat lactic bacteria composition of vaginitis and preparation method thereof.
Background technology
Vaginitis is colpitis.Intrusion of the characteristics of normal healthy women vagina is due to anatomical tissue to pathogen has certainly
Right defense function.Such as the closure of introitus, vagina front and rear wall is close to, hyperplasia of the vaginal epithelial cell under the influence of estrogen and
Cells of superficial layer angling, vagina acid-base degree keeps balance, is suppressed the breeding of the pathogen of adaptation alkalescence, and neck tube mucus is in
Alkalescence, when the natural defense function of vagina is destroyed, pathogen is easy to invade, and can cause colpitis.The hair of vaginitis
Existence is in many reasons, but main cause is that flora imbalance causes.It is normal to parasitize healthy women vaginal flora, lactobacillus
Dominance, Lactobacillus vaginalis play key effect to maintaining vagina normal flora.The glycogen of vagina epithelium through lactobacillus effect
Lactic acid is decomposed into, makes vagina locally in weakly acidic condition.Following situations are possible to influence the balance of vaginal ecosystem:1. property swashs
Element:Such as estrogen level reduction before and after the menstrual period, intravaginal pH is caused to rise the growth for being conducive to anaerobic bacteria.2. some spermicidals are sub
Contraceptive jelly to the toxic effect of lactobacillus.3. medicine:Perhaps lactobacillus can be killed or suppressed to multi-medicament such as broad-spectrum antibiotic
And influence the environment of intravaginal.4. infect;Such as the unrest of women's property, STD etc. is infected, may interfere with the original flora of intravaginal and cause to lose
Adjust.At present, use doctor trained in Western medicine antibiotic therapy, but effect not to feel quite pleased hospital, easily recurrence, very big pain is brought to patient more
It is bitter.Therefore, a kind of good effect is developed, recurrence is difficult and the low treatment method of toxic and side effect is prior art skill urgently to be resolved hurrily
Art problem.
The content of the invention
It is a kind of with significantly treatment vaginitis and without any present invention aim at providing to overcome the shortcoming of prior art
Lactic bacteria composition for the treatment of vaginitis of toxic and side effect and preparation method thereof.
The present invention is by following technical measures realization, a kind of lactic bacteria composition for treating vaginitis, including lactic acid bacteria
Combination core, the lactic acid bacteria combination core is wrapped with coated layer;The coated layer includes the composition of parts by weight:Oligomeric different malt
Sugar 30~50, protein substance 30~50, Cranberry fruit powder 5~10, nitrocellulose 5~10;The lactic acid bacteria combines core by such as
The bacterial strain composition of lower parts by weight:Lactobacillus salivarius 30~40, lactobacillus reuteri 20~30, Lactobacillus rhamnosus 10~20,
Lactobacterium acidophilus 10~20.
Used as a kind of preferred embodiment, the content of Lactobacillus salivarius is 10 in the lactic bacteria composition7~109Cuf/g, sieve
The content of Yi Shi lactobacillus is 106~108Cuf/g, the content of Lactobacillus rhamnosus are 106~107Cuf/g, lactobacterium acidophilus
Content is 106~107cuf/g。
As a kind of preferred embodiment, Lactobacillus salivarius, lactobacillus reuteri, rhamnose breast bar in the lactic acid bacteria combination core
Bacterium and lactobacterium acidophilus are resistant to 0.3% Pig cholate and acyclic acidic that pH is 2.5 respectively through resistance to Pig cholate, acidproof screening
Border.
Used as a kind of preferred embodiment, the lactobacillus reuteri is GL-104 bacterial strains, and the Lactobacillus salivarius is AP-32 bacterium
Strain, deposit number CCTCC NO:M2011127;The lactobacillus reuteri is TE-33 bacterial strains, deposit number CCTCC NO:
M2011126;The Lactobacillus rhamnosus is CT-53 bacterial strains, deposit number CCTCC NO:M2011129;The lactobacillus acidophilus
It is F-1 bacterial strains, deposit number CCTCC NO:M2011124.
Used as a kind of preferred embodiment, the protein substance is casein and lactoferrin layer, wherein casein and newborn iron egg
White weight ratio is 1-5:1-5.
Also a kind of preparation method of the lactic bacteria composition for treating vaginitis of the present invention, comprises the following steps:
(1) by Lactobacillus salivarius, lactobacillus reuteri, Lactobacillus rhamnosus, lactobacterium acidophilus respectively with 1ml/100ml's
Inoculum concentration is seeded in culture medium, at 35 DEG C~45 DEG C, cultivates 12~24h, the lactobacillus strain for being activated;
(2) the four kinds of strains that will be activated are with parts by weight:Lactobacillus salivarius 30~40, lactobacillus reuteri 20~30, mouse
After Lee's sugar lactobacillus 10~20, lactobacterium acidophilus 10~20 mix, then mixed bacteria is seeded to the inoculum concentration of 1ml/100ml
In nutrient solution, at 35 DEG C~45 DEG C, after 10~12h of culture, centrifuging and taking precipitation, after being incubated 6~10h at 4 DEG C~10 DEG C,
Matter, obtains combination strain;
(3) by parts by weight:Oligoisomaltose 30~50, protein substance 30~50, Cranberry fruit powder 5~10, nitre
Acid cellulose 5~10 is added water and is sufficiently mixed into coating buffer, and combination strain addition coating buffer is mixed evenly;
(4) liquid that will be mixed evenly solidifies in being added drop-wise to calcium chloride solution, and vacuum freeze-drying 12h obtains lactic acid bacteria after filtering
Composition.
Used as a kind of preferred embodiment, the content of Lactobacillus salivarius is 10 in the lactic bacteria composition7~109Cuf/g, sieve
The content of Yi Shi lactobacillus is 106~108Cuf/g, the content of Lactobacillus rhamnosus are 106~107Cuf/g, lactobacterium acidophilus
Content is 106~107cuf/g。
As a kind of preferred embodiment, the Lactobacillus salivarius, lactobacillus reuteri, Lactobacillus rhamnosus and lactobacterium acidophilus
Before activation respectively through resistance to Pig cholate, acidproof screening, 0.3% Pig cholate and sour environment that pH is 2.5 are resistant to.
Used as a kind of preferred embodiment, the Lactobacillus salivarius is AP-32 bacterial strains, deposit number CCTCC NO:M2011127;
The lactobacillus reuteri is TE-33 bacterial strains, deposit number CCTCC NO:M2011126;The Lactobacillus rhamnosus is CT-53
Bacterial strain, deposit number CCTCC NO:M2011129;The lactobacillus acidophilus is F-1 bacterial strains, deposit number CCTCC NO:
M2011124。
Used as a kind of preferred embodiment, the protein substance is casein and lactoferrin layer, wherein casein and newborn iron egg
White weight ratio is 1-5:1-5.
The present invention has the effect of significantly treatment vaginitis, and because being prebiotic becteriums product, does not have any poison to human body
Side effect.
Specific embodiment
The present invention is described in further detail with reference to embodiment.
Embodiment 1
(1) actication of culture
Filter out the Lactobacillus salivarius AP-32 bacterial strains of resistance to 0.3% Pig cholate, the sour environment that resistance to PH is 2.5, deposit number
CCTCC NO:M2011127;Lactobacillus reuteri TE-33 bacterial strains, deposit number CCTCC NO:M2011126;Rhamnose breast bar
Bacterium CT-53 bacterial strains, deposit number CCTCC NO:M2011129;Lactobacillus acidophilus F-1 bacterial strains, deposit number CCTCC NO:
M2011124.The four kinds of bacterium that will be filtered out are seeded in MRS culture mediums with the inoculum concentration of 1ml/100ml respectively, at 45 DEG C, training
Support 12h, the lactobacillus strain for being activated;
Wherein, the formula of MRS culture mediums is:10g peptones, 8g beef extract powders, 20g Portugals are added in 1000mL distilled water
Grape sugar, 4g yeast extracts, the water manganese sulfates of 0.05g tetra-, 1mL Tween 80s, 2g dipotassium hydrogen phosphates, 5g sodium acetates, 0.2g magnesium sulfate, 2g
Dibasic ammonium citrate;
(2) combination strain is prepared
The four kinds of strains that will be activated are with parts by weight:Lactobacillus salivarius 30g, lactobacillus reuteri 20g, Lactobacillus rhamnosus
After 10g, lactobacterium acidophilus 10g mixing, then mixed bacteria is seeded in nutrient solution with the inoculum concentration of 1ml/100ml, at 45 DEG C
Under, after culture 10h, with 6000r/min, at 5 DEG C, centrifugation 15min, taking precipitate, after being incubated 6h at 4 DEG C, homogeneous is obtained
Combination strain;
(3) coating combination strain is prepared
By following weight:Oligoisomaltose 30g, casein 15g, lactoferrin 15g, nitrocellulose 5g add water
1000ml is sufficiently mixed into coating buffer, and combination strain addition coating buffer is mixed evenly;
(4) lactic bacteria composition is prepared
The liquid that will be mixed evenly solidifies in being added drop-wise to calcium chloride solution, and vacuum freeze-drying 12h obtains lactic acid bacteria group after filtering
Compound.The content of Lactobacillus salivarius is 10 in lactic bacteria composition7~109Cuf/g, the content of lactobacillus reuteri are 106~
108Cuf/g, the content of Lactobacillus rhamnosus are 106~107Cuf/g, the content of lactobacterium acidophilus are 106~107cuf/g。
Embodiment 2
Filter out the Lactobacillus salivarius AP-32 bacterial strains of resistance to 0.3% Pig cholate, the sour environment that resistance to PH is 2.5, deposit number
CCTCC NO:M2011127;Lactobacillus reuteri TE-33 bacterial strains, deposit number CCTCC NO:M2011126;Rhamnose breast bar
Bacterium CT-53 bacterial strains, deposit number CCTCC NO:M2011129;Lactobacillus acidophilus F-1 bacterial strains, deposit number CCTCC NO:
M2011124.The four kinds of bacterium that will be filtered out are seeded in MRS culture mediums with the inoculum concentration of 1ml/100ml respectively, at 35 DEG C, training
Support 24h, the lactobacillus strain for being activated;
Wherein, the formula of MRS culture mediums is:10g peptones, 8g beef extract powders, 20g Portugals are added in 1000mL distilled water
Grape sugar, 5g yeast extracts, the water manganese sulfates of 0.06g tetra-, 1mL Tween 80s, 2g dipotassium hydrogen phosphates, 5g sodium acetates, 0.2g magnesium sulfate, 2g
Dibasic ammonium citrate;
(2) combination strain is prepared
The four kinds of strains that will be activated are with parts by weight:Lactobacillus salivarius 40g, lactobacillus reuteri 30g, Lactobacillus rhamnosus
After 20g, lactobacterium acidophilus 20g mixing, then mixed bacteria is seeded in nutrient solution with the inoculum concentration of 1ml/100ml, at 35 DEG C
Under, after culture 12h, with 4500r/min, at 10 DEG C, centrifugation 20min, taking precipitate, after being incubated 10h at 10 DEG C, homogeneous,
Obtain combination strain;
(3) coating combination strain is prepared
By following weight:Oligoisomaltose 50g, casein 35g, lactoferrin 35g, nitrocellulose 10g add water
1000ml is sufficiently mixed into coating buffer, and combination strain addition coating buffer is mixed evenly;
(4) lactic bacteria composition is prepared
The liquid that will be mixed evenly solidifies in being added drop-wise to calcium chloride solution, and vacuum freeze-drying 12h obtains lactic acid bacteria group after filtering
Compound.The content of Lactobacillus salivarius is 10 in lactic bacteria composition7~109Cuf/g, the content of lactobacillus reuteri are 106~
108Cuf/g, the content of Lactobacillus rhamnosus are 106~107Cuf/g, the content of lactobacterium acidophilus are 106~107cuf/g。
Embodiment 3
Filter out the Lactobacillus salivarius AP-32 bacterial strains of resistance to 0.3% Pig cholate, the sour environment that resistance to PH is 2.5, deposit number
CCTCC NO:M2011127;Lactobacillus reuteri TE-33 bacterial strains, deposit number CCTCC NO:M2011126;Rhamnose breast bar
Bacterium CT-53 bacterial strains, deposit number CCTCC NO:M2011129;Lactobacillus acidophilus F-1 bacterial strains, deposit number CCTCC NO:
M2011124.The four kinds of bacterium that will be filtered out are seeded in MRS culture mediums with the inoculum concentration of 1ml/100ml respectively, at 42 DEG C, training
Support 13h, the lactobacillus strain for being activated;
Wherein, the formula of MRS culture mediums is:10g peptones, 8g beef extract powders, 20g Portugals are added in 1000mL distilled water
Grape sugar, 4g yeast extracts, the water manganese sulfates of 0.05g tetra-, 1mL Tween 80s, 2g dipotassium hydrogen phosphates, 5g sodium acetates, 0.2g magnesium sulfate, 2g
Dibasic ammonium citrate;
(2) combination strain is prepared
The four kinds of strains that will be activated are with parts by weight:Lactobacillus salivarius 35g, lactobacillus reuteri 23g, Lactobacillus rhamnosus
After 15g, lactobacterium acidophilus 15g mixing, then mixed bacteria is seeded in nutrient solution with the inoculum concentration of 1ml/100ml, at 42 DEG C
Under, after culture 11h, with 7000r/min, at 5 DEG C, centrifugation 10min, taking precipitate, after being incubated 10h at 4 DEG C, homogeneous is obtained
To combination strain;
(3) coating combination strain is prepared
By parts by weight:Oligoisomaltose 35g, casein 20g, lactoferrin 20g, nitrocellulose 6g add water
1000ml is sufficiently mixed into coating buffer, and combination strain addition coating buffer is mixed evenly;
(4) lactic bacteria composition freeze-dried powder is prepared
The liquid that will be mixed evenly solidifies in being added drop-wise to calcium chloride solution, and vacuum freeze-drying 12h obtains lactic acid bacteria group after filtering
Compound freeze-dried powder.The content of Lactobacillus salivarius is 10 in lactic bacteria composition freeze-dried powder7~109Cuf/g, lactobacillus reuteri
Content be 106~108Cuf/g, the content of Lactobacillus rhamnosus are 106~107Cuf/g, the content of lactobacterium acidophilus are 106~
107cuf/g。
It is used to further illustrate the present invention below by clinical test.
1st, clinical observation
Using lactic bacteria composition freeze-dried powder of the present invention (being prepared according to the method for embodiment 1) treatment patient totally 240, pass through
Gynecologial examination is made a definite diagnosis, 20~48 years old its age, average 33 years old.It is randomly divided into two groups, control group 120, treatment group 120.
2nd, medicament selection
Control group using oral medicine woman inflammation capsule (Divine Land pharmacy), 0.4g/, one time 3,2 times a day, continuously take 30
My god;
Treatment group anthology invention lactic bacteria composition freeze-dried powder (is prepared) according to the method for embodiment 1, course for the treatment of (30 days
The course for the treatment of), 1.5g/ bags, two times a day, 2 bags every time, boiling water is taken after mixing it with water.
3rd, diagnostic criteria
Vaginal fluid increases, pruritus vulvue, cusalgia, can be with frequent micturition, odynuria and intercourse pain;Gynecologial examination finds vagina
Secretion increases, vagina mucosa it is congested or it is visible be dispersed in bleeding spot, posterior fornix of vagina has volume leukorrhea, in lark or yellowish green
Color purulent secretion, show bubble or white stiff curdled milk or bean dregs sample sometimes, the visible oedema of vulva, scratch, pattern erythema;
Vaginal fluid laboratory examination finds gemma and the mycelia of trichomonad or candidiasis.
4th, efficacy assessment standard
Cure:After treatment end, clinical symptoms are wholly absent;
Effectively:After treatment end, clinical symptoms partial disappearance, the state of an illness is alleviated substantially;
It is invalid:After treatment end, clinical symptoms are not taken on a new look substantially, and disease amelioration is not obvious.
Specific treatment results see the table below:
Group | Treatment number | Cure | Effectively | It is invalid | Total effective rate |
Control group | 120 | 32 | 39 | 49 | 59.2% |
Treatment group | 120 | 75 | 42 | 3 | 97.5% |
Conclusion:The cure rate for the treatment of group is 45%, and effective percentage is 90%, significantly larger than control group.
Model case
Case 1:Lee so-and-so, 50 years old.Main suit:Pruritus vulvue more than 1 year, with frequent micturition, odynuria and intercourse pain, takes Western medicine
Half a year is more, and recurrence is twice.Check and find, vaginal fluid increases, posterior fornix of vagina has volume leukorrhea, vaginal fluid chemical examination inspection
Look into discovery trichomonad.Take lactic bacteria composition freeze-dried powder of the present invention (being prepared according to the method for embodiment 1), a course for the treatment of, one day two
Secondary, 2 bags every time, boiling water is taken after mixing it with water, and after continuous 10 days, symptom disappears, and is continuing with consolidating within 20 days stabilization therapeutic effect;Follow-up 1 year
No relapse.
Case 2:Wang, 22 years old, main suit:Pruritus vulvue, cusalgia, vaginal fluid increase, after gynecologial examination finds vagina
Arched roof has a large amount of stiff curdled milk sample leukorrhea, and vaginal fluid checks the mycelia of visible candidiasis, is diagnosed as and examines mycotic the moon
Road is scorching.Take lactic bacteria composition freeze-dried powder of the present invention (according to the method for embodiment 1 prepare), a course for the treatment of, two times a day, every time
2 bags, boiling water is taken after mixing it with water, and after continuous 15 days, symptom disappears, and is continuing with consolidating within 15 days stabilization therapeutic effect;Follow-up half a year has no multiple
Hair.
Above is lactic bacteria composition of vaginitis and preparation method thereof is treated to the present invention being set forth, it is used to help
Understand the present invention, but embodiments of the present invention and be not restricted to the described embodiments, it is any without departing from institute under the principle of the invention
The change of work, modification, replacement, combination, simplify, should be equivalent substitute mode, be included in protection scope of the present invention it
It is interior.
Claims (10)
1. a kind of lactic bacteria composition for treating vaginitis, it is characterised in that:Core, the lactic acid bacteria combination are combined including lactic acid bacteria
Core is wrapped with coated layer;The coated layer includes the composition of parts by weight:Oligoisomaltose 30~50, protein substance 30~
50th, Cranberry fruit powder 5~10, nitrocellulose 5~10;The lactic acid bacteria combination core is made up of the bacterial strain of following parts by weight:
Lactobacillus salivarius 30~40, lactobacillus reuteri 20~30, Lactobacillus rhamnosus 10~20, lactobacterium acidophilus 10~20.
2. lactic bacteria composition according to claim 1, it is characterised in that:Lactobacillus salivarius in the lactic bacteria composition
Content be 107~109Cuf/g, the content of lactobacillus reuteri are 106~108Cuf/g, the content of Lactobacillus rhamnosus are 106
~107Cuf/g, the content of lactobacterium acidophilus are 106~107cuf/g。
3. lactic bacteria composition according to claim 1, it is characterised in that:Saliva breast bar in the lactic acid bacteria combination core
Bacterium, lactobacillus reuteri, Lactobacillus rhamnosus and lactobacterium acidophilus are resistant to 0.3% respectively through resistance to Pig cholate, acidproof screening
Pig cholate and sour environment that pH is 2.5.
4. lactic bacteria composition according to claim 1, it is characterised in that:The Lactobacillus salivarius is AP-32 bacterial strains, is protected
Hide numbering CCTCC NO:M2011127;The lactobacillus reuteri is TE-33 bacterial strains, deposit number CCTCC NO:
M2011126;The Lactobacillus rhamnosus is CT-53 bacterial strains, deposit number CCTCC NO:M2011129;The lactobacillus acidophilus
It is F-1 bacterial strains, deposit number CCTCC NO:M2011124.
5. lactic bacteria composition according to claim 1, it is characterised in that:The protein substance is casein and newborn iron egg
The weight ratio of white, wherein casein and lactoferrin is 1-5:1-5.
6. a kind of preparation method of the lactic bacteria composition for treating vaginitis, it is characterised in that comprise the following steps:
(1) by Lactobacillus salivarius, lactobacillus reuteri, Lactobacillus rhamnosus, lactobacterium acidophilus respectively with the inoculation of 1ml/100ml
Amount is seeded in culture medium, at 35 DEG C~45 DEG C, cultivates 12~24h, the lactobacillus strain for being activated;
(2) the four kinds of strains that will be activated are with parts by weight:Lactobacillus salivarius 30~40, lactobacillus reuteri 20~30, rhamnose
After lactobacillus 10~20, lactobacterium acidophilus 10~20 mix, then mixed bacteria is seeded to culture with the inoculum concentration of 1ml/100ml
In liquid, at 35 DEG C~45 DEG C, after 10~12h of culture, centrifuging and taking precipitation, after being incubated 6~10h at 4 DEG C~10 DEG C, homogeneous,
Obtain combination strain;
(3) by parts by weight:Oligoisomaltose 30~50, protein substance 30~50, Cranberry fruit powder 5~10, nitric acid are fine
Dimension element 5~10 is added water and is sufficiently mixed into coating buffer, and combination strain addition coating buffer is mixed evenly;
(4) liquid that will be mixed evenly solidifies in being added drop-wise to calcium chloride solution, and vacuum freeze-drying 12h obtains lactic acid bacteria combination after filtering
Thing.
7. preparation method according to claim 6, it is characterised in that:Lactobacillus salivarius contains in the lactic bacteria composition
Measure is 107~109Cuf/g, the content of lactobacillus reuteri are 106~108Cuf/g, the content of Lactobacillus rhamnosus are 106~
107Cuf/g, the content of lactobacterium acidophilus are 106~107cuf/g。
8. preparation method according to claim 6, it is characterised in that:The Lactobacillus salivarius, lactobacillus reuteri, sandlwood
Sugared lactobacillus and lactobacterium acidophilus respectively through resistance to Pig cholate, acidproof screening, are resistant to 0.3% Pig cholate and pH before activation
It is 2.5 sour environment.
9. preparation method according to claim 6, it is characterised in that:The Lactobacillus salivarius is AP-32 bacterial strains, and preservation is compiled
Number CCTCC NO:M2011127;The lactobacillus reuteri is TE-33 bacterial strains, deposit number CCTCC NO:M2011126;Institute
Lactobacillus rhamnosus is stated for CT-53 bacterial strains, deposit number CCTCC NO:M2011129;The lactobacillus acidophilus is F-1 bacterial strains,
Deposit number CCTCC NO:M2011124.
10. preparation method according to claim 6, it is characterised in that:The protein substance is casein and lactoferrin
Layer, wherein the weight ratio of casein and lactoferrin are 1-5:1-5.
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