CN106305797A - Biological pesticide with camellia oleifera roots and method for preparing biological pesticide - Google Patents
Biological pesticide with camellia oleifera roots and method for preparing biological pesticide Download PDFInfo
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- CN106305797A CN106305797A CN201610697403.5A CN201610697403A CN106305797A CN 106305797 A CN106305797 A CN 106305797A CN 201610697403 A CN201610697403 A CN 201610697403A CN 106305797 A CN106305797 A CN 106305797A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a biological pesticide with camellia oleifera roots and a method for preparing the biological pesticide, and relates to the technical field of biomedicine. The biological pesticide comprises effective components including camellia oleifera root extract A, camellia oleifera root extract B, folium pterocaryae stenopterae extract, paecilomyces lilacinus powder, radix boehmeriae extract, radix boehmeriae extract liquid, chelidonium majus extract liquid, aschersonia sp powder, hirsutella thompsonii powder, nitrated coccus powder, tea saponin and nerium oleander root extract. The method includes (1), preparing mixed microbial agents; (2), preparing mixtures A; (3), preparing mixtures B; (4), mixing the mixed microbial agents, the mixtures A and the mixtures B with one another. The biological pesticide and the method have the advantages that insects can be killed and expelled by the biological pesticide, the biological pesticide is antibacterial, antiviral and fully naturally botanical, is broad in spectrum and high in efficiency, is suitable for preventing and treating diseases and pests for various vegetables, melons, fruits and rice and can be used for preventing and treating plant diseases and insect pests for other plants, and the like.
Description
Technical field
The present invention relates to biomedicine technical field, particularly a kind of oil tea root biological pesticide and preparation method thereof.
Background technology
Corps diseases is one of principal element causing crop production reduction, and what disease was serious may result in the significantly underproduction,
And many crops can not continuous cropping plantation.Although, the preventing and treating of chemical pesticide has certain effect, but seriously polluted, and along with length
Phase uses, many bacterial resistance occurred, causes patient's condition to rise.Meanwhile, because the amount of executing of medicament is relatively big, cause crop that medicine occurs
Evil and intoxicating phenomenon, have a strong impact on agricultural production, more seriously in grain, veterinary antibiotics and all agricultural product, all
Having a large amount of noxious substance to remain, very harmful to people, animal, people, animal intoxicating phenomenon occurs often, has directly threatened human health.
In recent years, international and domestic green agriculture of greatly developing, advocate and use biological pesticide, replace now widely used chemical pesticide.
At present, the kind of biological pesticide mainly has: jingganmycin, antibiotic in pyrimidine nucleoside class, Ningnanmycin, macro tetrolide, agriculture resist
120, c-type creotoxin etc..
Chinese patent (number of patent application is 201410443738.5) is disclosed " a kind of nuisanceless antimicrobial peptide biological pesticide ",
Material including following parts by weight: earthworm antimicrobial peptide 40-50 part, Herba Houttuyniae 15-20 part, bean starch 5-17 part, phenethyl
Phenol polyethenoxy ether 15-19 part, corn straw powder 5-23 part, Paenibacillus polymyxa 3-9 part, oleifera dry cake 22-31 part, eight
Angle 5-8 part, Flos Caryophylli 10-14 part, oleum anisi 20-31 part, N,N'-dimethyl-.gamma..gamma.'-dipyridylium 13-20 part, water 90-150 part.
Another Chinese patent (number of patent application is 201410694549.5) disclosed " a kind of microbial pesticide antibacterial ",
Material including following parts by weight: Toosendanin 17-26 part, alkyl polyglucoside 9-49 part, methyl-benzyl polyoxyethylene ether 3-17 part,
Methanol 20-34 part, Radix Et Rhizoma Fagopyri Tatarici powder 25-41 part, card patuletin 9-11 part, Bacillus cercus powder 5-12 part, emamectin benzoate 25-
34 parts, leach camellia seed oil 20-30 part, bentonite 5-8 part, glycerol 14-26 part, seat shell cryptogam end 3-7 part, draw glue 5-9 part,
Bacillus subtilis 45-62 part, Mancozeb 3-5 part.
Summary of the invention
It is an object of the invention to provide one can parasite killing, anthelmintic, again can antibacterial, antiviral, and wide spectrum, efficiently, complete
Biological pesticide of natural plant and preparation method thereof.
For achieving the above object, the technical measures that the present invention is taked are a kind of oil tea root biological pesticides of invention, and it is effective
Composition includes the raw material of following weight portion:
Described oil tea root extract is to be prepared from by the following method:
First fresh oil tea root is cleaned, be cut into the thick uniform sheet of 0.1 0.45cm cm, then, put into supersound extraction
In device, add the low polar organic solvent of 1-3 times of weight, under frequency is 500-800Hz, processes 1-3h, filters, respectively
Collect oil tea root low polar organic solvent extracting solution and oil tea root extracts slag；Then, low for oil tea root polar organic solvent is extracted
Liquid is concentrated, dry, obtains oil tea root extract A；The oil tea root of 40-60 mesh is become to extract slag oil tea root extraction Slag treatment thinner
Material, is then added in the clear water of 2-4 times of weight, boils extraction 1.5-3.0h, filters, collects oil tea root Aqueous extracts；Again will
Oil tea root Aqueous extracts is concentrated, dry, obtains oil tea root extract B；
Described Radix Boehmeriae extract and Radix Boehmeriae extracting solution are to be prepared from by the following method:
First fresh Radix Boehmeriae is cleaned, be cut into the thick uniform sheet of 0.1 0.25cm, then, put into supersonic extractors
In, add 2-4 times of weight, volumetric concentration be 60-80% organic solvent solution in, under frequency is 10-20KHz locate
Reason 40-60min, filters, and collects Radix Boehmeriae organic solvent extraction liquid respectively and Radix Boehmeriae extracts slag；Then, Radix Boehmeriae is organic
Solvent extraction liquid vacuum be 5-10KPa, temperature be 40-50 DEG C at be concentrated into the 20-30% of original volume, obtain Radix Boehmeriae
Extracting solution；The clear water that Radix Boehmeriae extracts slag and 2-5 times of weight again boils extraction 0.5-1.5h, filters, obtains Radix Boehmeriae water and carry
Liquid；In Radix Boehmeriae Aqueous extracts, add the decolorising agent accounting for former Radix Boehmeriae weight 10-18% again, at 40-50 DEG C, process 10-
20min, filters, and must decolour Radix Boehmeriae Aqueous extracts；Again by concentrated for decolouring Radix Boehmeriae Aqueous extracts, dry, obtain Radix Boehmeriae extract.
Described bamboo Radix Persicae extract can be commercially available bamboo Radix Persicae extract commercial sources, it is also possible to be as follows
It is prepared from:
By disease-free, clean without the fresh Folium seu Cortex Nerii root rotted, be ground into the Folium seu Cortex Nerii root fines of 40-60 mesh, then with
The clear water of 2-4 times of weight boils extraction 2-4h together, filters, obtains Folium seu Cortex Nerii root Aqueous extracts；Again in Folium seu Cortex Nerii root Aqueous extracts
Add the decolorising agent accounting for former Folium seu Cortex Nerii root weight 15-20%, at 40-50 DEG C, process 10-30min, filter, obtain Folium seu Cortex Nerii
Root water carries destaining solution；Folium seu Cortex Nerii root water is carried destaining solution concentrated, dry again, obtain bamboo Radix Persicae extract.
Described Chinese Wingmut Leaf extract can be commercially available Chinese Wingmut Leaf extract commercial sources, it is also possible to be as follows
It is prepared from:
The fresh Chinese Wingmut Leaf that disease-free, nothing are rotted is cleaned, grinds to form uniform Chinese Wingmut Leaf slurry, then in Chinese Wingmut Leaf is starched
Add 1-3 times of weight, volumetric concentration is the organic solvent solution of 60-80%, soaks 4-7h, filter, obtain Chinese Wingmut Leaf under room temperature
Organic solvent extraction liquid；By concentrated for Chinese Wingmut Leaf organic solvent extraction liquid, dry, obtain Pterocarya Stenoptera cotyledon extract.
Described Herba Chelidonii extracting solution can be commercially available Herba Chelidonii extracting solution commercial sources, it is also possible to be as follows
It is prepared from:
The fresh Herba Chelidonii that disease-free, nothing are rotted is cleaned, with the warm water one that 1-3 times of weight, temperature are 50-60 DEG C
Rise and grind to form uniform Herba Chelidonii slurry, then by Herba Chelidonii slurry through the centrifuge of 5000-10000r/min, obtain Herba Chelidonii water
Immersion；Again by Herba Chelidonii infusion vacuum be 5-10KPa, temperature be 50-65 DEG C at be concentrated into the 20-of original volume
35%, obtain Herba Chelidonii extracting solution.
Described low polar organic solvent is one or more in ethyl acetate, petroleum ether, dichloromethane, when for two kinds
Time above, weight portion or other ratio such as the ratio of each component is.
Described decolorising agent is one or more in acid activated carbon, neutral kieselguhr, macro porous silica gel, when for two kinds with
Time upper, weight portion or other ratio such as the ratio of each component is.
Described organic solvent solution is one or more in methanol, ethanol, acetone, ethyl acetate, when for two kinds with
Time upper, weight portion or other ratio such as the ratio of each component is.
Its active ingredient composition proposal one optimized is the raw material including following weight portion:
Its active ingredient composition proposal two optimized is the raw material that active ingredient includes following weight portion:
Its active ingredient composition proposal three optimized is the raw material that active ingredient includes following weight portion:
The oil tea root biological pesticide of the present invention, its preparation method can be to be mixed by the raw material of above-mentioned active ingredient,
Can also be to prepare according to the following procedure:
(1), prepared by mix bacterium agent: oil tea root extract B, Folium seu Cortex Nerii root extract, Radix Boehmeriae extract are mixed together all
Even, form microbial inoculum compound；In microbial inoculum compound, be slowly added to clear water again, stirring while adding, cause hold can agglomerating time
Become the microbial inoculum compound that adds water；Then, Paecilomyces lilacinus powder, Tang Shi hirsutism mycopowder end, Aschersonia powder are joined
In water microbial inoculum compound, mix thoroughly, process 2-3 days at relative humidity 70-80%, temperature 28-48 DEG C, condition of normal pressure lower seal,
Add the mixing of nitrification coccus powder, obtain mix bacterium agent, standby；
(2), prepared by compound A: is mixed together uniformly by Herba Chelidonii extracting solution and Radix Boehmeriae extracting solution, then, is slowly added to
In tea saponin, limit edged stirs, and to mixing, obtains compound A, standby；
(3), prepared by compound B: be mixed together uniformly with Chinese Wingmut Leaf extract by oil tea root extract A, then by compound A
It is slowly added thereto, stirs evenly, be dried under the conditions of less than 55 DEG C to moisture content less than 8%, obtain compound B, standby；
(4), mixing: mix bacterium agent and compound B are mixed, obtains oil tea root biological pesticide.
The oil tea root biological pesticide of the present invention with clear water by 1:(300-700) part by weight mix after, water can be prepared as
Agent oil tea root biological pesticide.
The oil tea root biological pesticide of the present invention and solvent, emulsifying agent are by (10-30): (30-40): the weight of (10-20)
After ratio mixing, Emulsion oil tea root biological pesticide can be prepared as；Wherein: described solvent is ethanol, methanol, propylene glycol, fourth two
One or more in alcohol, when for time two or more, weight portion or other ratio such as the ratio of each component is；Described emulsifying agent
For one or more in dodecylbenzene sodium sulfonate, phenethyl phenol poly-oxypropylene polyoxyethylene ether, dibenzyl cumenyl phenol,
When for time two or more, weight portion or other ratio such as the ratio of each component is.
The oil tea root biological pesticide of the present invention and water, solvent, emulsifying agent, co-emulsifier are by (10-30): (20-30):
(5-15): (10-15): after the part by weight mixing of (5-10), aqueous emulsion oil tea root biological pesticide can be prepared as；Wherein: institute
The solvent stated is ethanol；Described emulsifying agent is dodecylbenzene sodium sulfonate, phenethyl phenol poly-oxypropylene polyoxyethylene ether, dibenzyl
One or more in base cumenyl phenol, when for time two or more, weight portion or other ratio such as the ratio of each component is；Institute
The co-emulsifier stated is isobutanol.
The oil tea root biological pesticide of the present invention, uses oil tea root extract, Chinese Wingmut Leaf extract, Paecilomyces lilacinus mycopowder
End, Radix Boehmeriae extract, Herba Chelidonii extracting solution, Aschersonia powder, Tang Shi hirsutism mycopowder end, nitrification coccus powder, tea saponin
With Folium seu Cortex Nerii root extract.
Oil tea root extract is that the root with oil tea (Camillia oleifera Abel) or root bark extract and form, and has clear
The effects such as thermal detoxification, regulating QI to relieve pain, promoting blood circulation and detumescence.
Chinese Wingmut Leaf extract is to extract with the leaf of Pterocarya Stenoptera (Pterocarya stenoptera C.DC) to form, and kills
Worm, Detoxication, have preferable curative effect to ulcer.
Paecilomyces lilacinus powder is with Paecilomyces lilacinus [Paecilomyces lilacinus (Thom.) Samson]
Processing, Paecilomyces lilacinus is applicable to the crops such as control of nematode, root knot nematode disease preventing and treating, plant root-knot nematodes preventing and treating
Root knot nematode disease is prevented and treated, such as crop root-knot nematode, cyst lines such as crops Semen sojae atricolor, Fructus Lycopersici esculenti, Nicotiana tabacum L., Fructus Cucumidis sativi, Citrullus vulgaris, Fructus Solani melongenae, Rhizoma Zingiberis Recens
The preventing and treating of worm.
Radix Boehmeriae extract is to extract with the root of Boehmeria (Radix Boehmeriae) to form, and has clearing away heat and promoting diuresis, removing toxic substances
Effect, acylate and alkaloid contained by Radix Boehmeriae all have inhibitory action to gram positive bacteria and negative bacterium, wherein in vitro
Hemolytic streptococcus, streptococcus pneumoniae, escherichia coli, anthrax bacillus are extremely sensitive to acylate；Salmonella is to alkaloid height
Sensitive.
Herba Chelidonii extracting solution is to extract with Herba Chelidonii (Chelidonium majus Linn.) Herb to form, and has removing toxic substances, goes out
Bacterium, insecticidal action, especially have certain killing action to Pieris rapae and brevicoryne brassicae.
Aschersonia powder is to process by Aschersonia (Aschersonia), and Aschersonia can cause aleyrodid and Jie
The popular death of shell worm.
Tang Shi hirsutism mycopowder end is to process with the many trichobacterias of Tang Shi (Hirsutella thompsonii), is that one is posted
Natural disposition compares fungus widely, and it can infect various crop insect, evil demodicid mite, can be used to kill the primary pest rust in citrus culture district
Tick, moreover it is possible to kill the insects such as red spider.
Nitrification coccus powder is to process with nitrification coccus (Nitrococcus), helps out, works as pesticide residues
Time, Nitromonas decomposes Ammonia material and forms nitrate, forms sour environment and makes fungus inactivate.
Tea saponin (Tea Saponin), has another name called TS, has certain stomach toxicity and stronger deterrent effect to Pieris rapae,
And concentration is the highest, deterrent is the strongest, and preventing and treating Pieris rapae harm cabbage is had certain effect；Landscape flower is used as insecticide prevent
Control subterranean pest-insect, such as the insect such as cutworm, nematicide.Also Pomacea canaliculata and Limax, oncomelania etc. to hazard rice all has good poison
Kill effect.
Folium seu Cortex Nerii root extract is to extract with the root of Folium seu Cortex Nerii (Nerium indicum Mill.) to form, and Folium seu Cortex Nerii is extracted
Liquid can be used for eliminating spiral shell and suppression Caulis Zizaniae caduciflorae striped rice borer and the biological activity of nematicide.
The oil tea root biological pesticide being prepared from by above-mentioned many natural plant components, can kill as cutworm,
The insect pest such as nematicide, Aranea, can prevent and treat again the disease such as anthrax, soft rot, thus, there is wide spectrum, the characteristic such as efficiently.
The oil tea root biological pesticide of the present invention, once carried out Oil Tea Anthracnose controlling experiment:
Anthrax is the multiple disease of oil tea, and Oil Tea Anthracnose can bring withered alabastrum, flower bud trace, sick fruit, sick fruit stem, sick leaf
Bud, sick branch such as are survived the winter at the situation.This disease also can infect the tissues such as oil tea young fruit, the camellia oleifera fruit poor growth falling ill light, sick fruit deformity；
May occur in which pink sorus time serious, cause camellia oleifera fruit to come off, not only cause the underproduction then, and owing to branch development is bad,
Carry out annual production also can be affected.
This test is selected in anthrax and occurs serious camellia oleifera lam (oil tea plantation, Lingling District, Yongzhou City, Hubei Province) to carry out,
If 2 chemicals treatment, respectively 400 times of liquid of Bordeaux mixture and oil tea root biological pesticide (preparing according to embodiment) of the present invention
500 times of liquid, every preventing and treating in 10 days once, preventing and treating 3 times continuously.With clear water for comparison.
According to test survey, the sick tree rate of clear water check plot anthrax and disease index sustainable growth, branch is in a bad way, leaf
Sheet is dried-up even to come off；And using the tea oil tree of above-mentioned 2 kinds of pesticide, the state of an illness has all obtained preferable control.Wherein second time medicine
Latter 7 days, the preventive effect with the oil tea root biological pesticide of the present invention was optimal, reaches 96.04%, is better than the preventive effect of Bordeaux mixture
90.14%；After medicine 10 days for the third time, the preventive effect of the oil tea root biological pesticide of the present invention reached 98.4%, is better than Bordeaux mixture
Preventive effect 94.26%.
Result of the test shows, Oil Tea Anthracnose is all had the most anti-by the oil tea root biological pesticide of the present invention, Bordeaux mixture
Effect, the preventive effect of the oil tea root biological pesticide of the present invention is better than the preventive effect of Bordeaux mixture.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.Description below is in the way of enumerating, but this
Bright protection domain is not limited thereto.
The oil tea root biological pesticide of the present embodiment, its preparation process is as follows:
(1), prepared by active ingredient raw material:
1., prepared by oil tea root extract A and oil tea root extract B:
First the fresh oil tea root that disease-free, nothing are rotted is cleaned, be cut into the thick uniform sheet of 0.25cm, then, put into
In supersonic extractors, add the ethyl acetate of 2 times of weight, under frequency is 650Hz, processes 2h, filters, collect oil tea respectively
Root low polar organic solvent extracting solution and oil tea root extract slag；Then, by concentrated for low for oil tea root polar organic solvent extracting solution,
It is dried, obtains oil tea root extract A；Become the oil tea root of 50 mesh to extract slag fines oil tea root extraction Slag treatment again, be then added to 3
In the clear water of times weight, boil extraction 2.5h, filter, collect oil tea root Aqueous extracts；Again by concentrated for oil tea root Aqueous extracts, dry
Dry, obtain oil tea root extract B；
2., prepared by Radix Boehmeriae extract and Radix Boehmeriae extracting solution:
First the fresh Radix Boehmeriae that disease-free, nothing are rotted is cleaned, be cut into the thick uniform sheet of 0.15cm, then, put into
In supersonic extractors, add 3 times of weight, volumetric concentration be 70% ethanol solution in, under frequency is 15KHz process
50min, filters, and collects Radix Boehmeriae organic solvent extraction liquid respectively and Radix Boehmeriae extracts slag；Then, Radix Boehmeriae organic solvent is carried
Take liquid vacuum be 8KPa, temperature be at 45 DEG C, to be concentrated into the 25% of original volume, obtain Radix Boehmeriae extracting solution；Again Radix Boehmeriae is carried
The clear water taking slag and 4 times of weight boils extraction 1.2h, filters, obtains Radix Boehmeriae Aqueous extracts；Add in Radix Boehmeriae Aqueous extracts again and account for
The neutral kieselguhr of former Radix Boehmeriae weight 15%, processes 15min at 45 DEG C, filters, and must decolour Radix Boehmeriae Aqueous extracts；Again will be de-
Color Radix Boehmeriae Aqueous extracts is concentrated, dry, obtains Radix Boehmeriae extract.
3., prepared by bamboo Radix Persicae extract:
By disease-free, clean without the fresh Folium seu Cortex Nerii root rotted, be ground into the Folium seu Cortex Nerii root fines of 50 mesh, then with 3 times
The clear water of weight boils extraction 3h together, filters, obtains Folium seu Cortex Nerii root Aqueous extracts；Add in Folium seu Cortex Nerii root Aqueous extracts again and account for former folder
The acid activated carbon of bamboo Radix Persicae weight 18%, processes 20min at 45 DEG C, filters, obtain Folium seu Cortex Nerii root water and carry destaining solution；Again will folder
It is concentrated, dry that bamboo Radix Persicae water carries destaining solution, obtains bamboo Radix Persicae extract.
4., prepared by Chinese Wingmut Leaf extract:
The fresh Chinese Wingmut Leaf that disease-free, nothing are rotted is cleaned, grinds to form uniform Chinese Wingmut Leaf slurry, then in Chinese Wingmut Leaf is starched
Adding 2 times of weight, volumetric concentrations is the methanol solution of 70%, soaks 6h, filter, obtain Chinese Wingmut Leaf organic solvent extraction under room temperature
Liquid；By concentrated for Chinese Wingmut Leaf organic solvent extraction liquid, dry, obtain Pterocarya Stenoptera cotyledon extract.
5., prepared by Herba Chelidonii extracting solution:
The fresh Herba Chelidonii that disease-free, nothing are rotted is cleaned, grinds together with the warm water that 2 times of weight, temperature are 55 DEG C
Uniformly Herba Chelidonii slurry, then by Herba Chelidonii slurry through the centrifuge of 8000r/min, obtain Herba Chelidonii infusion；To bend in vain again
Dish infusion vacuum be 8KPa, temperature be at 60 DEG C, to be concentrated into the 28% of original volume, obtain Herba Chelidonii extracting solution.
(2), get the raw materials ready:
Take respectively according to following weight portion:
(3), preparation:
1., prepared by mix bacterium agent: oil tea root extract B, Folium seu Cortex Nerii root extract, Radix Boehmeriae extract are mixed together all
Even, form microbial inoculum compound；In microbial inoculum compound, be slowly added to clear water again, stirring while adding, cause hold can agglomerating time
Become the microbial inoculum compound that adds water；Then, Paecilomyces lilacinus powder, Tang Shi hirsutism mycopowder end, Aschersonia powder are joined
In water microbial inoculum compound, mix thoroughly, process 3 days at relative humidity 75%, temperature 38 DEG C, condition of normal pressure lower seal, add nitrification
Coccus powder mixes, and obtains mix bacterium agent, standby；
2., prepared by compound A: is mixed together uniformly by Herba Chelidonii extracting solution and Radix Boehmeriae extracting solution, then, is slowly added to
In tea saponin, limit edged stirs, and to mixing, obtains compound A, standby；
3., prepared by compound B: be mixed together uniformly with Chinese Wingmut Leaf extract by oil tea root extract A, then by compound A
It is slowly added thereto, stirs evenly, be dried under the conditions of less than 55 DEG C to moisture content less than 8%, obtain compound B, standby；
4., mixing: mix bacterium agent and compound B are mixed, obtains oil tea root biological pesticide.
When needing the oil tea root biological pesticide using water preparation, oil tea root biological pesticide prepared by the present embodiment and clear water
By 1:400 part by weight mix after, water preparation oil tea root biological pesticide.
When needing the oil tea root biological pesticide using Emulsion, oil tea root biological pesticide prepared by the present embodiment and second
Alcohol, dodecylbenzene sodium sulfonate press 15:35:15 part by weight mixing after, Emulsion oil tea root biological pesticide.
When need use aqueous emulsion oil tea root biological pesticide time, oil tea root biological pesticide prepared by the present embodiment with
After water, ethanol, phenethyl phenol poly-oxypropylene polyoxyethylene ether, isobutanol press the part by weight mixing of 15:25:10:12:8,
Aqueous emulsion oil tea root biological pesticide.
The oil tea root biological pesticide of the present invention, it is adaptable to the insect pest preventing and controlling of various vegetables, melon and fruit and Oryza sativa L., can be used for other
Plant pest management.
Claims (8)
1. an oil tea root biological pesticide, it is characterised in that active ingredient includes the raw material of following weight portion:
Described oil tea root extract is to be prepared from by the following method:
First fresh oil tea root is cleaned, be cut into the thick uniform sheet of 0.1 0.45cm, then, put in supersonic extractors, then
Add the low polar organic solvent of 1-3 times of weight, under frequency is 500-800Hz, processes 1-3h, filters, collect oil respectively
Tea root low polar organic solvent extracting solution and oil tea root extract slag；Then, by low for oil tea root polar organic solvent extracting solution through dense
Contract, be dried, obtain oil tea root extract A；The oil tea root of 40-60 mesh is become to extract slag fines, then oil tea root extraction Slag treatment again
Join in the clear water of 2-4 times of weight, boil extraction 1.5-3.0h, filter, collect oil tea root Aqueous extracts；Again by oil tea root water
Extract is concentrated, dry, obtains oil tea root extract B；
Described Radix Boehmeriae extract and Radix Boehmeriae extracting solution are to be prepared from by the following method:
First fresh Radix Boehmeriae is cleaned, be cut into the thick uniform sheet of 0.1 0.25cm, then, put in supersonic extractors, then
Add 2-4 times of weight, volumetric concentration be 60-80% organic solvent solution in, under frequency is 10-20KHz process 40-
60min, filters, and collects Radix Boehmeriae organic solvent extraction liquid respectively and Radix Boehmeriae extracts slag；Then, Radix Boehmeriae organic solvent is carried
Take liquid vacuum be 5-10KPa, temperature be 40-50 DEG C at be concentrated into the 20-30% of original volume, obtain Radix Boehmeriae extracting solution；
The clear water that Radix Boehmeriae extracts slag and 2-5 times of weight again boils extraction 0.5-1.5h, filters, obtains Radix Boehmeriae Aqueous extracts；Again to
Radix Boehmeriae Aqueous extracts adds the decolorising agent accounting for former Radix Boehmeriae weight 10-18%, at 40-50 DEG C, processes 10-20min, mistake
Filter, must decolour Radix Boehmeriae Aqueous extracts；Again by concentrated for decolouring Radix Boehmeriae Aqueous extracts, dry, obtain Radix Boehmeriae extract.
Oil tea root biological pesticide the most according to claim 1, it is characterised in that described bamboo Radix Persicae extract is by following
Method is prepared from:
Fresh Folium seu Cortex Nerii root is cleaned, is ground into the Folium seu Cortex Nerii root fines of 40-60 mesh, then with the clear water one of 2-4 times of weight
Rise and boil extraction 2-4h, filter, obtain Folium seu Cortex Nerii root Aqueous extracts；Add in Folium seu Cortex Nerii root Aqueous extracts again and account for former Folium seu Cortex Nerii root weight
The decolorising agent of amount 15-20%, processes 10-30min at 40-50 DEG C, filters, obtain Folium seu Cortex Nerii root water and carry destaining solution；Again will folder
It is concentrated, dry that bamboo Radix Persicae water carries destaining solution, obtains bamboo Radix Persicae extract.
Oil tea root biological pesticide the most according to claim 1, it is characterised in that described Chinese Wingmut Leaf extract is by following
Method is prepared from:
Fresh Chinese Wingmut Leaf is cleaned, grinds to form uniform Chinese Wingmut Leaf slurry, then in Chinese Wingmut Leaf is starched, add 1-3 times of weight, volume
Concentration is the organic solvent solution of 60-80%, soaks 4-7h, filter, obtain Chinese Wingmut Leaf organic solvent extraction liquid under room temperature；By maple
Folium Populi Pseudo-simonii organic solvent extraction liquid is concentrated, dry, obtains Pterocarya Stenoptera cotyledon extract.
Oil tea root biological pesticide the most according to claim 1, it is characterised in that described Herba Chelidonii extracting solution is by following
Method is prepared from:
Fresh Herba Chelidonii is cleaned, grinds to form uniform white in the wrong together with the warm water that 1-3 times of weight, temperature are 50-60 DEG C
Puree, then by Herba Chelidonii slurry through the centrifuge of 5000-10000r/min, obtain Herba Chelidonii infusion；Again by Herba Chelidonii water logging
Liquid vacuum be 5-10KPa, temperature be 50-65 DEG C at be concentrated into the 20-35% of original volume, obtain Herba Chelidonii extracting solution.
Oil tea root biological pesticide the most according to claim 1, it is characterised in that: described low polar organic solvent is acetic acid
One or more in ethyl ester, petroleum ether, dichloromethane, when for time two or more, the ratio of each component is etc. weight portion or its
Its ratio.
Oil tea root biological pesticide the most according to claim 1 and 2, it is characterised in that: described decolorising agent is acid activity
One or more in charcoal, neutral kieselguhr, macro porous silica gel, when for time two or more, the ratio of each component is etc. weight portion or
Other ratio.
7. according to the oil tea root biological pesticide described in claim 1 or 3, it is characterised in that: described organic solvent solution is first
One or more in alcohol, ethanol, acetone, ethyl acetate, when for time two or more, the ratio of each component is etc. weight portion or its
Its ratio.
8. the preparation method of oil tea root biological pesticide as according to any one of claim 1-4, it is characterised in that preparation
Process is as follows:
(1), prepared by mix bacterium agent: be mixed together uniformly by oil tea root extract B, Folium seu Cortex Nerii root extract, Radix Boehmeriae extract,
Form microbial inoculum compound；In microbial inoculum compound, be slowly added to clear water again, stirring while adding, cause hold can agglomerating time add
Water microbial inoculum compound；Then, Paecilomyces lilacinus powder, Tang Shi hirsutism mycopowder end, Aschersonia powder are joined the bacterium that adds water
In agent compound, mix thoroughly, process 2-3 days at relative humidity 70-80%, temperature 28-48 DEG C, condition of normal pressure lower seal, then add
Enter the mixing of nitrification coccus powder, obtain mix bacterium agent, standby；
(2), prepared by compound A: is mixed together uniformly by Herba Chelidonii extracting solution and Radix Boehmeriae extracting solution, then, is slowly added into tea
In Saponin, limit edged stirs, and to mixing, obtains compound A, standby；
(3), prepared by compound B: be mixed together uniformly with Chinese Wingmut Leaf extract by oil tea root extract A, then by slow for compound A
It is added thereto, stirs evenly, be dried under the conditions of less than 55 DEG C to moisture content less than 8%, obtain compound B, standby；
(4), mixing: mix bacterium agent and compound B are mixed, obtains oil tea root biological pesticide.
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| CN201610697403.5A CN106305797A (en) | 2016-08-19 | 2016-08-19 | Biological pesticide with camellia oleifera roots and method for preparing biological pesticide |
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Cited By (4)
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| CN106982564A (en) * | 2017-02-24 | 2017-07-28 | 安徽榈源农业科技有限公司 | A kind of implantation methods for improving wax gourd bacteriostatic activity |
| CN107372653A (en) * | 2017-08-03 | 2017-11-24 | 山东省农业科学院农产品研究所 | Medicament and the application of snail are prevented and treated in officinal dendrobium stem plantation production |
| CN108142445A (en) * | 2018-02-09 | 2018-06-12 | 湖北川玉茶业有限公司 | A kind of plant pesticide and preparation method and application |
| CN113180065A (en) * | 2021-05-27 | 2021-07-30 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Application of tartary buckwheat sprout extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106982564A (en) * | 2017-02-24 | 2017-07-28 | 安徽榈源农业科技有限公司 | A kind of implantation methods for improving wax gourd bacteriostatic activity |
| CN107372653A (en) * | 2017-08-03 | 2017-11-24 | 山东省农业科学院农产品研究所 | Medicament and the application of snail are prevented and treated in officinal dendrobium stem plantation production |
| CN107372653B (en) * | 2017-08-03 | 2019-12-03 | 山东省农业科学院农产品研究所 | Medicament and the application of snail are prevented and treated in officinal dendrobium stem plantation production |
| CN108142445A (en) * | 2018-02-09 | 2018-06-12 | 湖北川玉茶业有限公司 | A kind of plant pesticide and preparation method and application |
| CN113180065A (en) * | 2021-05-27 | 2021-07-30 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Application of tartary buckwheat sprout extract |
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