CN106190843B - A kind of multisample culture apparatus - Google Patents
A kind of multisample culture apparatus Download PDFInfo
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- CN106190843B CN106190843B CN201610701445.1A CN201610701445A CN106190843B CN 106190843 B CN106190843 B CN 106190843B CN 201610701445 A CN201610701445 A CN 201610701445A CN 106190843 B CN106190843 B CN 106190843B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention discloses a kind of multisample culture apparatus, including pedestal and several culture vessels, each culture vessel stacks, and culture vessel includes ware body, column, convex block and liquid injection pipe, and ware body top end surface is equipped with ware inverted draw cut, base top is equipped with base inverted draw cut, the convex block of one culture vessel of bottom is in base inverted draw cut, and a rotation pipe passes through all columns, and rotation pipe can be relatively rotated with column, in sealing contact between rotation pipe lateral wall and column madial wall, rotation pipe lateral wall is equipped with sump pit；Column is equipped with the equal liquid hole with being connected inside ware body, and cell filtering net and equal liquid check valve are equipped in equal liquid hole, is connected inside liquid injection pipe lower end and ware body；The convex block of top culture vessel is at the bottom in the ware inverted draw cut of culture vessel.The beneficial effects of the invention are as follows：Suitable for cell culture and measure, comparison, rationally and stable structure, operating process is more convenient, and the adjusting consistency during testing is good, accuracy is high for space layout.
Description
Technical field
The invention belongs to technical field of cell culture more particularly to a kind of multisample culture apparatus.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.The life of cell
Length needs nutrient environment, and the nutrient matrix for maintaining cell growth is known as culture medium.Culture medium can be divided by its physical state
Fluid nutrient medium and solid medium.Cell culture technology is more for testing at present other than being applied to production（It is such as right
Group sample parameter is measured, and be compared and study）, and test in it is more commonly used to be fluid nutrient medium.It is in addition, thin
Born of the same parents' culture needs to use cell culture apparatus, common are culture dish, incubator etc., and the cell culture under general environment can be straight
It connects using culture dish, if desired for some particular surroundings（It is such as powered, heats）, then special incubator can be used.Exist at present
When carrying out cell culture and Comparability test in the process, although common culture apparatus can play provides carrying for culture medium
The function in space, but in space layout reasonability, integrated operation convenience, regulating effect consistency etc., be still short of.
Invention content
It is suitable for cell culture and measure, comparison it is an object of the invention to overcome the defect of prior art, and provide a kind of,
Space layout rationally and stable structure, operating process is more convenient, the training that the adjusting consistency during testing is good, accuracy is high
Support device.
To achieve these goals, the present invention uses following technical scheme：
A kind of multisample culture apparatus, the culture vessel including pedestal and several upper end openings, each culture vessel by down toward
On stack gradually, a culture vessel of bottom contact pedestal, the culture vessel includes ware body, is arranged on bottom in ware body
On column, the convex block that is arranged on the external bottom of ware and be arranged on the liquid injection pipe on ware body sidewall and being in tilted layout, convex block is horizontal
Rectangular in cross-section, ware body top end surface are equipped with the ware inverted draw cut being adapted to convex block, and base top is equipped with the base inverted draw cut being adapted to convex block,
The convex block of one culture vessel of bottom is in base inverted draw cut, and column upper/lower terminal is open, a rotation arranged vertically
Pipe can be relatively rotated across all columns, the rotation pipe with column, and be sealed between rotation pipe lateral wall and column madial wall
Contact, rotation pipe lateral wall are equipped with the sump pit through rotation pipe upper/lower terminal；
In a culture vessel：Column is equipped with the equal liquid hole with being connected inside ware body, and cell mistake is equipped in equal liquid hole
It is connected inside strainer, liquid injection pipe lower end and ware body, ware body sidewall is equipped with the tune amount transverse tube with being connected inside ware body, tune amount transverse tube
The tune amount column through two pipe ends of tune amount transverse tube is equipped with, tune amount column is threadedly coupled with tune amount transverse tube, and tune amount column and tune amount are horizontal
Pipe is coaxial, and tune amount column inner end is in ware body, and tune amount column outer end is in outside tune amount transverse tube, tune amount column side wall be equipped with baseline trough and
The alignment mark rod being in outside tune amount transverse tube, tune amount transverse tube are equipped with baseline rod；
In two neighboring culture vessel：The convex block of top culture vessel is at the bottom in the ware inverted draw cut of culture vessel；
The column includes cylinder and orifice plate, and cylinder lateral wall is equipped with side opening, on a column：Orifice plate is stuck in side opening
Interior, in sealing contact between orifice plate and side opening hole wall, orifice plate is made of mutually symmetrical with upper half plate with lower half plate, upper half plate and lower half
Plate is an integral molding structure, and equal liquid hole is located on upper half plate；
The liquid injection pipe upper end is equipped with pipe lid with liquid injection pipe thread fitting, is arranged in rotation pipe for by the top
The vessel cover that the upper end opening of one culture vessel seals.
Preferably, the pedestal includes matrix and several support columns being arranged below matrix, matrix, which is equipped with, to be run through
The bottom through-hole on the upper and lower surface of matrix, rotation pipe lower end are stretched into the through-hole of bottom, and fluid cylinder is connect equipped with opening up below the through-hole of bottom,
The bottom bracing plate being connect at least one support column is equipped with below matrix, bottom bracing plate is equipped with the cylinder slot being adapted to connecing fluid cylinder bottom,
Fluid cylinder bottom is connect to be in a slot.
Preferably, being equipped with core pipe and several horizontally disposed diaphragm plates in the rotation pipe, core pipe lower end is stretched into bottom and is led to
In hole, support ring is arranged in core pipe, support ring is in higher than core pipe bottom and support ring in the through-hole of bottom, and branch is equipped in the through-hole of bottom
Seat is supportted, seat slot and the seat apical ring slot being adapted to rotation pipe lower end are equipped at the top of support base, contact seat slot slot bottom in support ring bottom turns
Dynamic pipe lower end is stretched into an apical ring slot and rotation bottom of the tube contact seat apical ring slot slot bottom, rotation pipe are rotatably assorted with support base, core
Pipe is rotatably assorted across support base and core pipe with support base, and diaphragm plate is set in core pipe, and diaphragm plate cross section is in circular ring shape, is turned
Dynamic pipe, core pipe and each diaphragm plate are coaxial, and core pipe can be relatively rotated with diaphragm plate, in sealing contact between core pipe and diaphragm plate, horizontal
It seals and fixes between partition board and rotation pipe；
Diaphragm plate is equipped with even lumen pore, and core pipe is equipped with several hole sealing plates for the company's of sealing lumen pore, hole sealing plate and tabula
Plate corresponds, in corresponding hole sealing plate and diaphragm plate：Hole sealing plate is in above diaphragm plate, hole sealing plate bottom surface contact diaphragm plate
Top surface；
Quantitative chamber is formed in rotation pipe between adjacent two pieces of diaphragm plates, if rotation pipe is equipped with main pipe to fluid apertures, quantitative chamber
Fluid apertures one-to-one correspondence is given with pipe, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber and culture vessel：On column
Equipped with cylinder to fluid apertures, quantitative chamber is connected to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is to fluid apertures and cylinder to fluid apertures in circle
Cylindricality, and the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder gives fluid apertures axis horizontal, and quantitative chamber lowest part is given higher than cylinder
Fluid apertures lowest part, cylinder are higher than equal liquid hole highest point to fluid apertures lowest part.
Preferably, in corresponding quantitative chamber and culture vessel：Quantitative intracavitary is equipped with tune amount liquid capsule, is set in tune amount liquid capsule
It is useful for the liquid supply pipe of tune amount liquid capsule feed flow, liquid supply pipe one end is connected with tune amount liquid capsule, and liquid supply pipe is by being in quantitative intracavitary
Hard tube section and the outer segment being in outside quantitative chamber are formed；
Pedestal is used to supply fluid cylinder to liquid supply pipe feed flow equipped with several, includes cylinder body for fluid cylinder, is slidingly sealed with cylinder body
The feed flow piston of cooperation and the piston rod for connecting and being in above feed flow piston with feed flow piston, the slidably direction of feed flow piston
For upper and lower directions, supplied in fluid cylinder at one：Space in cylinder body below feed flow piston is sap cavity, in cylinder body above feed flow piston
Space is adjusting cavity；
Sap cavity is corresponded with liquid supply pipe, and the liquid supply pipe other end is connected with corresponding sap cavity, and liquid supply pipe passes through rotation pipe side
Wall and rotation pipe lower end seal between liquid supply pipe and rotation pipe side wall and fix.
Preferably, the pedestal is equipped with is equipped with horizontally disposed tune with the fixed adjustment seat of pedestal, adjustment seat lower section
Plate is saved, for piston rod across adjustable plate, piston rod is equipped with upper holder block and lower drawing block, and adjustment seat is equipped with vertical arrangement and passes through and adjusts
The homophony pole of seat is saved, homophony pole is threadedly coupled with adjustment seat, and homophony pole is equipped with upper holder block and lower drawing block, upper holder block bottom
Face contact adjusts plate top surface, and lower drawing block top surface contact adjustable plate bottom surface, homophony pole top is equipped with and the fixed master of homophony pole
Adjusting knob.
Preferably, the homophony pole is equipped with the instruction disk being in above adjustment seat, indicate that disk and homophony pole are same
Axis, adjustment seat are equipped with the indicating arm of slot with a scale, and indicating arm is arranged vertically and indicating arm contact instruction disk side wall.
Preferably, be equipped with the sealing compression bar arranged vertically in the rotation pipe, sealing compression bar pass through all diaphragm plates and
It being threadedly coupled between sealing compression bar and all diaphragm plates, sealing compression bar is equipped with several pressure plates for being used to compress hole sealing plate,
Pressure plate is corresponded with hole sealing plate, and pressure plate is in the top of corresponding hole sealing plate.
The beneficial effects of the invention are as follows：Suitable for cell culture and measure, comparison, space layout rationally and stable structure,
Operating process is more convenient, and the adjusting consistency during testing is good, accuracy is high.
Description of the drawings
Fig. 1 is the structure diagram of the present invention；
Fig. 2 is the structure diagram of orifice plate of the present invention；
Fig. 3 is the enlarged drawing at A in Fig. 1；
Fig. 4 is the enlarged drawing at B in Fig. 1；
Fig. 5 is the enlarged drawing at C in Fig. 1；
Fig. 6 is the enlarged drawing at D in Fig. 1；
Fig. 7 is a sectional view at rotation pipe of the present invention.
In figure：Pedestal 1 connects fluid cylinder 12, bottom bracing plate 13, support base 14, matrix 1a, support column 1b, bottom through-hole 1c, incubator
Ware 2, cylinder are to fluid apertures 21, equal liquid hole 22, pipe lid 23, vessel cover 24, orifice plate 25, tune amount transverse tube 26, tune amount column 27, alignment mark rod
28th, baseline rod 29, ware body 2a, column 2b, convex block 2c, liquid injection pipe 2d, rotation pipe 3, sump pit 31, even diaphragm plate 32, lumen pore
321st, pipe to fluid apertures 33, tune amount liquid capsule 34, feed flow funnel 35, quantitative chamber 3a, core pipe 4, support ring 41, hole sealing plate 42, for fluid cylinder
5th, cylinder body 5a, feed flow piston 5b, piston rod 5c, sap cavity 5d, adjusting cavity 5e, adjustment seat 6, adjustable plate 61, upper holder block 611, drop-down
Block 612, homophony pole 62, main regulation knob 621, instruction disk 622, indicating arm 63, sealing compression bar 7, pressure plate 71.
Specific embodiment
The present invention will be further described with reference to the accompanying drawings and detailed description.
In embodiment as shown in Figures 1 to 7, a kind of multisample culture apparatus, including pedestal 1 and several upper end openings
Culture vessel 2, each culture vessel stack gradually from the bottom to top, the culture vessel contact pedestal of bottom, the incubator
Ware includes ware body 2a, the column 2b being arranged in ware body on bottom, the convex block 2c being arranged on the external bottom of ware and is arranged on ware
On body sidewall and the liquid injection pipe 2d that is in tilted layout, convex block cross section is rectangular, and ware body top end surface is equipped with the ware that is adapted to convex block
Inverted draw cut, base top are equipped with the base inverted draw cut being adapted to convex block, and the convex block of a culture vessel of bottom is in base inverted draw cut, erect
Cylinder upper/lower terminal is open, and a rotation pipe 3 arranged vertically passes through all columns, and the rotation pipe opposite can turn with column
Dynamic and in sealing contact between rotation pipe lateral wall and column madial wall, rotation pipe lateral wall is equipped with through rotation pipe upper and lower two
The sump pit 31 at end；
In a culture vessel：Column is equipped with the equal liquid hole 22 with being connected inside ware body, and cell is equipped in equal liquid hole
It is connected inside filter screen, liquid injection pipe lower end and ware body, ware body sidewall is equipped with the tune amount transverse tube 26 with being connected inside ware body, tune amount
Transverse tube is equipped with the tune amount column 27 through two pipe ends of tune amount transverse tube, and tune amount column is threadedly coupled with tune amount transverse tube, and tune amount column and
Tune amount transverse tube is coaxial, and tune amount column inner end is in ware body, and tune amount column outer end is in outside tune amount transverse tube, and tune amount column side wall is equipped with base
Quasi- slot and the alignment mark rod 28 being in outside tune amount transverse tube, tune amount transverse tube are equipped with baseline rod 29；
In two neighboring culture vessel：The convex block of top culture vessel is at the bottom in the ware inverted draw cut of culture vessel.
Before experiment, one of bottom is set to train when each culture vessel can be stacked gradually from the bottom to top placement, and place
It supports in the convex block insertion base inverted draw cut of vessel, the convex block of top culture vessel is made to be embedded in the ware inverted draw cut of lower section culture vessel, make to erect
Outside jacket casing to rotation pipe（Structure in rotation pipe and rotation pipe can be pre-set）, so that each culture vessel can be aligned
And it is fixed, the culture vessel of top just covers the culture vessel of lower section.Then liquid feeding is carried out to each liquid injection pipe（Add in liquid
Culture medium or culture solution）, without deliberately control liquid volume added during liquid feeding, as long as liquid level lid crosses equal liquid hole.Whole liquid feedings are completed
Afterwards, turn rotation pipe allows sump pit to be connected with each equal liquid hole, so that the liquid in each culture vessel can be from equal liquid hole stream
Go out, remaining liquid measure reaches consistent in culture vessel, then can turn rotation pipe, sump pit is allowed to leave equal liquid hole（It is made not connect
It is logical）, liquid feeding has so just been rapidly completed.And can also be realized if liquid measure by turn tune amount column in desired adjusting ware body,
And mode is very various：It such as, can be inside by tune amount column before liquid feeding（Outside）Turn certain distance, so then entire liquid feeding walks
After the completion of rapid（After the process of drain being carried out using equal liquid hole and sump pit）The liquid measure finally left in ware body can be reduced（Increase
Add）；For another example, after liquid feeding, sump pit is with equal liquid hole when connecting, the inside turn certain distance of tune amount column can be continued to discharge
A part of liquid in ware body, so as to which the liquid measure left be allowed in ware body to reduce.In above-mentioned regulative mode, the latter is more commonly used, this
Once being because after the completion of liquid feeding, with the mode drawn, toppled over, drain is relatively time-consuming again, and operation is also pretty troublesome, still
It carried out drain with aforementioned manner, adjusted, then it is very convenient.And the quantitative control mode of tune amount column displacement distance is also very more
Sample is given one example：In the present embodiment, at the beginning, can allow alignment mark rod be aligned with baseline rod, baseline trough and tune amount transverse tube it is remote
One end alignment from ware body, can be set as reference position herein（Baseline trough can also be allowed to be aligned in other setting positions, depending on making
Depending on the considering when demand of used time, design）, after liquid feeding, sump pit is with equal liquid hole when connecting, and tune amount column is per inside turn one
Circle, alignment mark rod will turn one enclose and be aligned again with baseline rod（It is aligned mark rod, the baseline rod person of being convenient to operation carries out
Observation and adjusting）, after being enclosed due to the inside turn one of tune amount column, the volume stretched into ware body is definite value（This value design when just
It has determined that）, so the liquid measure of discharge is also definite value.In addition, when culture, experiment, tend not to be that all tune fluid columns are all used
It arrives or each tune fluid column turn distance can be different, this is to adapt to different samples（Variety classes cell may may require that not
Same initial incubation liquid measure）Or quickly provide different culture environments（With cell types with identical or different Culture liquid measure come
Carry out culture and subsequent measurements, Comparability test）And the structure designed.It is noted that alignment mark rod is preferably provided at ware body
Outside, because being once arranged in ware body, alignment mark rod easily has an impact liquid level when rotating, and degree of regulation can be by certain
It influences, and is unfavorable for observing（It if culture vessel is opaque, then can cause not observing, if culture vessel is transparent, see
Examine also not as good as alignment mark rod be arranged on ware it is external when it is convenient）.
It should be pointed out that cell to be cultivated can just be placed in culture vessel before culture solution is added in（It needs to wait to cultivate
Cell of short duration can leave culture solution and non-inactivation or death）, at this time due to there is the presence of cell filtering net, cell will not flow out
Culture vessel；It can also be added in culture solution, equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that liquid in each ware body
Measure consistent process）Cell to be cultivated is added later；Also the culture solution that band directly can be needed to be cultivated to cell is directly injected into training
Support vessel（Experiment under the premise of this should not consider the influence of initial cell quantity, and due to the presence for having cell filtering net, cell
It will not flow out culture vessel）.It can specifically be selected according to test objective, demand.
The liquid injection pipe upper end is equipped with pipe lid 23 with liquid injection pipe thread fitting, is arranged in rotation pipe for by the top
A culture vessel the vessel cover 24 that seals of upper end opening.Pipe lid can block rotation pipe and extraneous connection, vessel cover
A culture vessel of the top and extraneous connection can be blocked, remaining culture vessel is then by a nearest training of itself top
Vessel are supported to block and extraneous connection.So as to ensure that each culture vessel separates during cell culture, experiment with the external world,
So as to which the present invention be allowed to be applicable to the experiment under the premise of being isolated from the outside world.
The pedestal includes matrix 1a and several support column 1b being arranged below matrix, and matrix is equipped with through matrix
The bottom through-hole 1c on upper and lower surface, rotation pipe lower end are stretched into the through-hole of bottom, and fluid cylinder 12 is connect equipped with opening up below the through-hole of bottom,
The bottom bracing plate 13 being connect at least one support column is equipped with below matrix, bottom bracing plate is equipped with the cylinder being adapted to connecing fluid cylinder bottom
Slot connects fluid cylinder bottom and is in a slot.The liquid discharged from sump pit can be recycled easily（It falls into and connects fluid cylinder）, wave can be avoided
Take and avoid polluting the external world.
The column includes cylinder and orifice plate 25, and cylinder lateral wall is equipped with side opening, on a column：Orifice plate is stuck in side opening
Interior, in sealing contact between orifice plate and side opening hole wall, orifice plate is made of mutually symmetrical with upper half plate with lower half plate, upper half plate and lower half
Plate is an integral molding structure, and equal liquid hole is located on upper half plate.In addition to equal liquid hole, orifice plate is structure symmetrical above and below, thus,
One piece of orifice plate can be achieved with the Liquid level of two kinds of different heights, that is, by orifice plate by upper half plate in upper and lower half plate in the form of lower
Side opening is caught in, then by equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）
Afterwards, liquid level is consistent with equal liquid hole lowest part in culture vessel（More than equal liquid hole is in the middle part of orifice plate at this time）；And by orifice plate
By upper half plate under, lower half plate side opening is caught in the form of upper, then liquid level and equal liquid hole lowest part in final culture vessel
Unanimously（Equal liquid hole is in following in the middle part of orifice plate at this time）.Furthermore, it is possible to which more set orifice plates are configured, there was only equal liquid hole between each set orifice plate
Height is different, so can be achieved with the quick control of various different initial liquid measures in culture vessel（Which liquid level is needed, just
A set of orifice plate corresponding with the liquid level is caught in advance and is used in the side opening of each culture vessel）.
Core pipe 4 is equipped in the rotation pipe and several horizontally disposed diaphragm plates 32, core pipe lower end are stretched into the through-hole of bottom, core
Support ring 41 is arranged on pipe, support ring is in higher than core pipe bottom and support ring in the through-hole of bottom, and support base is equipped in the through-hole of bottom
14, support base top is equipped with seat slot and the seat apical ring slot being adapted to rotation pipe lower end, support ring bottom contact seat slot slot bottom, rotation
Pipe lower end is stretched into an apical ring slot and rotation bottom of the tube contact seat apical ring slot slot bottom, rotation pipe are rotatably assorted with support base, core pipe
It is rotatably assorted across support base and core pipe with support base, diaphragm plate is set in core pipe, and diaphragm plate cross section is in circular ring shape, is rotated
Pipe, core pipe and each diaphragm plate are coaxial, and core pipe can be relatively rotated with diaphragm plate, in sealing contact between core pipe and diaphragm plate, tabula
It seals and fixes between plate and rotation pipe；
Diaphragm plate be equipped with even lumen pore 321, core pipe be equipped with several hole sealing plates 42 for the company's of sealing lumen pore, hole sealing plate and
Diaphragm plate corresponds, in corresponding hole sealing plate and diaphragm plate：Hole sealing plate is in above diaphragm plate, and the contact of hole sealing plate bottom surface is horizontal
Partition board top surface；
Quantitative chamber 3a is formed in rotation pipe between adjacent two pieces of diaphragm plates, it is fixed if rotation pipe is equipped with main pipe to fluid apertures 33
It measures chamber and gives fluid apertures one-to-one correspondence with pipe, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber and culture vessel：It is perpendicular
Cylinder is equipped with cylinder to fluid apertures 21, and quantitative chamber is connected to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is to fluid apertures and cylinder to fluid apertures
It is cylinder, and the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder gives fluid apertures axis horizontal, and quantitative chamber lowest part is high
Fluid apertures lowest part is given in cylinder, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part；
The feed flow funnel 35 being threadedly coupled with rotation pipe is equipped at the top of rotation pipe.During each structure installation, cooperation, if
Feed flow funnel can interfere, then can first unload feed flow funnel, after the completion of to be assembled, operation, put in back feed flow funnel, and after
It is continuous to carry out subsequent operation.
When needs carry out each culture vessel mid-term liquid feeding simultaneously（New culture solution, Ke Yiyu are added in incubation
Initial incubation liquid type, proportioning are identical, can also be different from initial incubation liquid type, proportioning）When, for each ware body, liquid
The time difference that body adds in is smaller, and the interference of suffered external cause is also smaller when finally measuring（It is preferably capable of adding in culture simultaneously
Liquid）.But it unless more people is allowed to carry out liquid feeding, and to ensure height while property to each culture vessel simultaneously, can be only achieved more
Ideal effect, and in practical operation, the feasibility of this mode is relatively low.And in the present solution, first turn rotation pipe, allowing pipe
It is staggered to fluid apertures with corresponding cylinder between fluid apertures（It does not connect）, then allow rotation pipe motionless, first turn core pipe（If rotation pipe with
Frictional force is sufficiently large between column, then can direct turn core pipe, at this time rotation pipe will not be driven；If it rubs between rotation pipe and column
It is limited to wipe power, then rotation pipe can be pinched on the other hand, the other hand carrys out turn core pipe；The present invention is in design, preferential former feelings
Shape）, hole sealing plate is driven to leave even lumen pore（Allow the sealing plate not company's of sealing lumen pore）, liquid feeding, culture then can be carried out at the top of rotation pipe
Liquid can pass through each even lumen pore, and finally then proceed to make rotation pipe motionless, turn core pipe makes envelope full of all quantitative chambers
Orifice plate seals even lumen pore, separates between quantitative chamber each at this time, and each quantitative intracavitary liquid measure is consistent.Then can turn rotation pipe, lead to
Crossing diaphragm plate drives core pipe to rotate together（It is fixed between diaphragm plate and rotation pipe, although can phase between core pipe and diaphragm plate
To rotation, but necessarily there is the power compressed mutually due in sealing contact between core pipe and diaphragm plate, between core pipe and diaphragm plate
And frictional force, so if relatively rotating, need to apply the external force that them is allowed to relatively rotate.And only turn core pipe when, rubbing
Under the action of wiping power, diaphragm plate can together be rotated with core pipe, so relative position is constant between rotation pipe and core pipe）, allow
Pipe is connected to fluid apertures with corresponding cylinder between fluid apertures, at this time each quantify chamber can give corresponding ware body inside simultaneously, equivalent
Liquid feeding can thoroughly eliminate during intermediate liquid feeding the disturbing factors such as the liquid feeding time started is inconsistent, liquid feeding duration is inconsistent.This
Outside, this programme is in addition to can be used for mid-term liquid feeding, it can also be used to liquid feeding when initially.And after having support base, it is arranged from sump pit
The liquid gone out can first reach at the top of support base, then flow down outward.
It is noted that when sump pit is connected with equal liquid hole, pipe is staggered to fluid apertures with corresponding cylinder between fluid apertures；Manage to
Fluid apertures with corresponding cylinder to being connected between fluid apertures when, sump pit is staggered with equal liquid hole.And pass through equal hydraulic control amount（I.e. mentioned by equal
Fluid apertures carries out drain so that the consistent process of liquid measure in each ware body）Afterwards, in the behaviour using quantitative chamber to progress liquid feeding in ware body
During work（During turn rotation pipe）, it is impossible to sump pit is allowed to be connected again with equal liquid hole, otherwise can lead to the leakage at sump pit
（Liquid flows out again from equal liquid hole, sump pit in ware body）, there are many kinds of specific constructive forms and mode of operation, in this measure two
Example,
Example 1：In the circumferential direction of rotation pipe, sump pit, equal liquid hole, pipe to fluid apertures, cylinder to fluid apertures along up time pointing needle successively
Arrangement.Equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）During operation, row
Liquid bath is clockwise to alignment equal liquid hole with rotation pipe（The process middle pipe is not connected to fluid apertures with corresponding cylinder to fluid apertures）,
After the completion of hydraulic control amount, sump pit continues to rotate clockwise and be staggered with equal liquid hole, at this time pipe to fluid apertures and corresponding cylinder to fluid apertures according to
So do not reach connected state, if then continuing turn rotation pipe clockwise, pipe can be allowed to be connected to fluid apertures with corresponding cylinder to fluid apertures；
Example 2：In the circumferential direction of rotation pipe, equal liquid hole, sump pit, pipe to fluid apertures, cylinder to fluid apertures along clockwise direction successively
Arrangement, equal hydraulic control amount（Drain is carried out mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）During operation, row
Liquid bath is rotated counterclockwise with rotation pipe to alignment equal liquid hole（The process middle pipe is not connected to fluid apertures with corresponding cylinder to fluid apertures）,
After the completion of hydraulic control amount, sump pit is rotated clockwise and is staggered with equal liquid hole, and pipe does not have still to fluid apertures with corresponding cylinder to fluid apertures at this time
Have and reach connected state, if then continuing turn rotation pipe clockwise, pipe can be allowed to be connected to fluid apertures with corresponding cylinder to fluid apertures.
In corresponding quantitative chamber and culture vessel：Quantitative intracavitary is equipped with tune amount liquid capsule 34, and tune amount liquid capsule is equipped with and is used for
To the liquid supply pipe of tune amount liquid capsule feed flow, liquid supply pipe one end is connected with tune amount liquid capsule, and liquid supply pipe is by being in the hard tube section of quantitative intracavitary
And the outer segment being in outside quantitative chamber is formed；
Pedestal be equipped with it is several be used for liquid supply pipe feed flow supply fluid cylinder 5, for fluid cylinder include cylinder body 5a, with cylinder body slip it is close
It seals the feed flow piston 5b of cooperation and is connect with feed flow piston and be in the piston rod 5c above feed flow piston, feed flow piston is slided
Dynamic direction is upper and lower directions, is supplied in fluid cylinder at one：Space in cylinder body below feed flow piston is sap cavity 5d, and feed flow is lived in cylinder body
Square space is adjusting cavity 5e beyond the Great Wall；
Sap cavity is corresponded with liquid supply pipe, and the liquid supply pipe other end is connected with corresponding sap cavity, and liquid supply pipe passes through rotation pipe side
Wall and rotation pipe lower end seal between liquid supply pipe and rotation pipe side wall and fix.
The pedestal is equipped with adjustment seat 6 fixed with pedestal, and horizontally disposed adjustable plate 61 is equipped with below adjustment seat, living
For stopper rod across adjustable plate, piston rod is equipped with upper holder block 611 and lower drawing block 612, and adjustment seat is equipped with vertical arrangement and passes through and adjusts
The homophony pole 62 of seat is saved, homophony pole is threadedly coupled with adjustment seat, and homophony pole is equipped with upper holder block and lower drawing block, upper holder block
Bottom surface contact adjusts plate top surface, and lower drawing block top surface contact adjustable plate bottom surface, homophony pole top is equipped with fixed with homophony pole
Main regulation knob 621.
The homophony pole is equipped with the instruction disk 622 being in above adjustment seat, indicates that disk and homophony pole are coaxial, adjusts
Seat is equipped with the indicating arm 63 of slot with a scale, and indicating arm is arranged vertically and indicating arm contact instruction disk side wall.
As previously mentioned, the present invention can be to carrying out equivalent while liquid feeding in each ware body.And in the present solution, can also be right
Liquid volume added is regulated and controled.At the beginning, tune amount liquid capsule is vacuum, if specified rate chamber is full of at this time, liquid volume added is exactly the reality of quantitative chamber
Border volume.It, can be with turn homophony pole when needing to control liquid volume added（Main regulation knob）, homophony pole is made to move upward or downward
It is dynamic, for moving down：Homophony pole drives adjustable plate to move down vertically by the upper holder block on homophony pole at this time（Upper holder block
Rotation, adjustable plate do not turn）, adjustable plate pushes down on each feed flow piston by each lower drawing block on piston rod, the liquid in each sap cavity
Body（Can be water or other liquid, as long as in stable condition, the structures such as the pipeline of process will not be damaged）Injection pair
The tune amount liquid capsule answered, at this point, the actual volume of quantitative chamber（Actual volume refers to accommodate the volume of culture solution）Become smaller,
And the numerical value specifically to become smaller, it can be read from indicating arm（Scale slot on indicating arm can mark and the practical appearance of quantitative chamber
The corresponding number of product, digital occurrence can be converted when manufacturing and designing with measured in advance.Bid can be done on instruction disk
Note, convenient for the reading being directly aligned according to label come mark-sense, alternatively, instruction disk can also be used as label to make in itself
With such as using indicating that disk top surface or bottom surface be used as label）.The above process can become smaller the actual volume of quantitative chamber, so as to
Control liquid volume added（Make its reduction）；Conversely, homophony pole is allowed to move up, then the liquid in tune amount liquid capsule can be extracted out, so as to
Controllable liquid volume added（Make its increase）.It is noted that liquid supply pipe is by being in the hard tube section of quantitative intracavitary and being in outside quantitative chamber
Outer segment form, wherein outer segment cannot be perfect rigidity, to ensure that diaphragm plate, core pipe etc. being capable of normal rotations（Core pipe
It is connect with outer segment, when rotating core pipe, diaphragm plate, outer segment is also required for certain locomotivity）, outer segment can
To select the pipe of the types such as sebific duct, oil cylinder petroleum pipeline.
The sealing compression bar 7 arranged vertically is equipped in the rotation pipe, sealing compression bar passes through all diaphragm plates and sealing compression bar
It is threadedly coupled between all diaphragm plates, sealing compression bar is equipped with several pressure plates 71 for being used to compress hole sealing plate, pressure plate
It is corresponded with hole sealing plate, pressure plate is in the top of corresponding hole sealing plate.As previously mentioned, liquid is filled it up in rotation pipe, Ran Houfeng
After orifice plate seals even lumen pore, separate between each quantitative chamber.But, in order to allow hole sealing plate will can the thorough company's of sealing lumen pore, hole sealing plate
Mutual extruding between one piece of diaphragm plate of lower contact is inevitable larger.After being used for multiple times, easily cause due to abrasion is larger
Sealing is insufficient.And in the present solution, hole sealing plate is sealed even after lumen pore, compression bar can be sealed with turn, pressure plate is driven to move down and press
Tight corresponding hole sealing plate, further improves leakproofness, ensures the partition effect between each quantitative chamber.And there are sealing compression bar, pressure
After tight plate, the too tight of pressure is not had to during fabrication, between hole sealing plate and diaphragm plate（Leakproofness can be protected by the pushing of pressure plate
Barrier）, so as to effectively reduce abrasion, prolong the service life.It is noted that after having sealing compression bar, pressure plate, turn allowing
Before the motionless and turn core pipe of dynamic pipe, pressure plate should be allowed to be not located at the state for compressing corresponding hole sealing plate, otherwise will appear " turning motionless "
Situation.
Claims (6)
1. a kind of multisample culture apparatus, it is characterized in that, the culture vessel including pedestal and several upper end openings, each culture vessel
It stacks gradually from the bottom to top, the culture vessel contact pedestal of bottom, the culture vessel includes ware body, is arranged on ware body
Column on interior bottom, the convex block being arranged on the external bottom of ware and the liquid injection pipe on ware body sidewall and being in tilted layout is arranged on,
Convex block cross section is rectangular, and ware body top end surface is equipped with the ware inverted draw cut being adapted to convex block, and base top is equipped with what is be adapted to convex block
Base inverted draw cut, the convex block of a culture vessel of bottom are in base inverted draw cut, and column upper/lower terminal is open, a vertical cloth
The rotation pipe put passes through all columns, and the rotation pipe can be relatively rotated with column, and rotation pipe lateral wall and column madial wall
Between it is in sealing contact, rotation pipe lateral wall be equipped with through rotation pipe upper/lower terminal sump pit；
In a culture vessel：Column is equipped with the equal liquid hole with being connected inside ware body, and cell filtering net is equipped in equal liquid hole,
It is connected inside liquid injection pipe lower end and ware body, ware body sidewall is equipped with the tune amount transverse tube with being connected inside ware body, is set on tune amount transverse tube
There is the tune amount column through two pipe ends of tune amount transverse tube, tune amount column is threadedly coupled with tune amount transverse tube, and tune amount column and tune amount transverse tube are same
Axis, tune amount column inner end are in ware body, and tune amount column outer end is in outside tune amount transverse tube, and tune amount column side wall is equipped with baseline trough and is in
Alignment mark rod outside tune amount transverse tube, tune amount transverse tube are equipped with baseline rod；
In two neighboring culture vessel：The convex block of top culture vessel is at the bottom in the ware inverted draw cut of culture vessel；
The column includes cylinder and orifice plate, and cylinder lateral wall is equipped with side opening, on a column：Orifice plate is stuck in side opening, hole
In sealing contact between plate and side opening hole wall, orifice plate is made of mutually symmetrical with upper half plate with lower half plate, and upper half plate is with lower half plate
Integrated formed structure, equal liquid hole are located on upper half plate；
The liquid injection pipe upper end is equipped with the pipe lid with liquid injection pipe thread fitting, is arranged in rotation pipe for by one of the top
The vessel cover that the upper end opening of culture vessel seals；
Core pipe is equipped in the rotation pipe and several horizontally disposed diaphragm plates, core pipe lower end are stretched into the through-hole of bottom, covered in core pipe
Equipped with support ring, support ring is in higher than core pipe bottom and support ring in the through-hole of bottom, and support base, support base top are equipped in the through-hole of bottom
Portion is equipped with seat slot and the seat apical ring slot being adapted to rotation pipe lower end, and support ring bottom contact seat slot slot bottom, rotation pipe lower end is stretched into
In seat apical ring slot and rotation bottom of the tube contact seat apical ring slot slot bottom, rotation pipe are rotatably assorted with support base, and core pipe passes through support base
And core pipe is rotatably assorted with support base, diaphragm plate is set in core pipe, diaphragm plate cross section be in circular ring shape, rotation pipe, core pipe and
Each diaphragm plate is coaxial, and core pipe can be relatively rotated with diaphragm plate, in sealing contact between core pipe and diaphragm plate, diaphragm plate and rotation pipe
Between seal fix；
Diaphragm plate is equipped with even lumen pore, and core pipe is equipped with several hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One corresponds to, in corresponding hole sealing plate and diaphragm plate：Hole sealing plate is in above diaphragm plate, hole sealing plate bottom surface contact diaphragm plate top
Face；
Quantitative chamber is formed in rotation pipe between adjacent two pieces of diaphragm plates, if rotation pipe is equipped with main pipe to fluid apertures, quantitative chamber and pipe
Fluid apertures one-to-one correspondence is given, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber and culture vessel：Column is equipped with
Cylinder is to fluid apertures, and quantitative chamber is connected to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is cylinder to fluid apertures with cylinder to fluid apertures,
And the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder gives fluid apertures axis horizontal, and quantitative chamber lowest part is higher than cylinder to fluid apertures
Lowest part, cylinder are higher than equal liquid hole highest point to fluid apertures lowest part.
2. a kind of multisample culture apparatus according to claim 1, it is characterized in that, the pedestal includes matrix and several sets
The support column below matrix is put, matrix is equipped with the bottom through-hole through the upper and lower surface of matrix, and bottom through-hole is stretched into rotation pipe lower end
It is interior, the opening up fluid cylinder that connects is equipped with below the through-hole of bottom, the bottom bracing plate being connect at least one support column, bottom are equipped with below matrix
Fagging is equipped with the cylinder slot being adapted to connecing fluid cylinder bottom, connects fluid cylinder bottom and is in a slot.
3. a kind of multisample culture apparatus according to claim 1, it is characterized in that, in corresponding quantitative chamber and culture vessel
In：Quantitative intracavitary is equipped with tune amount liquid capsule, and tune amount liquid capsule is equipped with for the liquid supply pipe to tune amount liquid capsule feed flow, liquid supply pipe one end with
Tune amount liquid capsule connects, and liquid supply pipe is made of the outer segment for being in the hard tube section of quantitative intracavitary and being in outside quantitative chamber；
Pedestal is used to supply fluid cylinder to liquid supply pipe feed flow equipped with several, includes cylinder body for fluid cylinder, is slidingly sealed cooperation with cylinder body
Feed flow piston and the piston rod that connect and be in above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction is supplied at one in fluid cylinder：Space in cylinder body below feed flow piston is sap cavity, the space in cylinder body above feed flow piston
For adjusting cavity；
Sap cavity and liquid supply pipe correspond, and the liquid supply pipe other end is connected with corresponding sap cavity, liquid supply pipe across rotation pipe side wall and
Rotation pipe lower end seals between liquid supply pipe and rotation pipe side wall and fixes.
4. a kind of multisample culture apparatus according to claim 3, it is characterized in that, the pedestal is equipped with and is fixed with pedestal
Adjustment seat, be equipped with horizontally disposed adjustable plate below adjustment seat, piston rod passes through adjustable plate, piston rod be equipped with upper holder block and
Lower drawing block, adjustment seat are equipped with vertical arrangement and across the homophony poles of adjustment seat, and homophony pole is threadedly coupled with adjustment seat, main
Adjusting rod is equipped with upper holder block and lower drawing block, and the contact of upper holder block bottom surface adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface,
It is equipped with and the fixed main regulation knob of homophony pole at the top of homophony pole.
5. a kind of multisample culture apparatus according to claim 4, it is characterized in that, the homophony pole, which is equipped with, is in tune
The instruction disk above seat is saved, indicates that disk and homophony pole are coaxial, adjustment seat is equipped with the indicating arm of slot with a scale, and indicating arm is vertical
Arrangement and indicating arm contact instruction disk side wall.
6. a kind of multisample culture apparatus according to claims 1 or 2 or 3, it is characterized in that, it is equipped in the rotation pipe perpendicular
The sealing compression bar directly arranged, sealing compression bar are threadedly coupled across all diaphragm plates and sealing compression bar and all diaphragm plates,
Sealing compression bar is equipped with several for compressing the pressure plates of hole sealing plate, and pressure plate is corresponded with hole sealing plate, and pressure plate is in pair
Answer the top of hole sealing plate.
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| CN201610701445.1A CN106190843B (en) | 2016-08-22 | 2016-08-22 | A kind of multisample culture apparatus |
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|---|---|---|---|
| CN201610701445.1A CN106190843B (en) | 2016-08-22 | 2016-08-22 | A kind of multisample culture apparatus |
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| CN106190843B true CN106190843B (en) | 2018-06-15 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106190843B (en) * | 2016-08-22 | 2018-06-15 | 刘力伟 | A kind of multisample culture apparatus |
| CN106222087B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of sample culture carrier |
| CN106282017B (en) * | 2016-08-22 | 2018-09-21 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106222089B (en) * | 2016-08-22 | 2018-09-21 | 王铁苗 | A kind of experiment auxiliary culture apparatus |
| CN106222090B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment assistant carrier |
| CN106047703B (en) * | 2016-08-22 | 2018-08-10 | 青岛爱博生物科技有限公司 | A kind of test sample culture apparatus |
| CN106467893B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment auxiliary device |
| CN106244457B (en) * | 2016-08-22 | 2018-08-03 | 日照安维信生物科技有限公司 | A kind of experiment culture carrier structure |
| CN107711304B (en) * | 2017-10-31 | 2019-09-13 | 四川明峰农业开发有限公司 | One plant stereo cultivation apparatus |
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