CN106119110A - A kind of culture carrier structure - Google Patents
A kind of culture carrier structure Download PDFInfo
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- CN106119110A CN106119110A CN201610699550.6A CN201610699550A CN106119110A CN 106119110 A CN106119110 A CN 106119110A CN 201610699550 A CN201610699550 A CN 201610699550A CN 106119110 A CN106119110 A CN 106119110A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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Abstract
The invention discloses a kind of culture carrier structure, including base and some culture vessels, each culture vessel stacks gradually, culture vessel includes ware body, column and liquid injection pipe, on column, the equal opening in lower two ends, one rotates pipe through all columns, rotate pipe can relatively rotate with column, and be in sealing contact between rotation pipe and column, rotate pipe lateral wall and be provided with sump pit, in a culture vessel: be provided with the equal liquid hole connected with portion in ware body on column, cell filtration net and equal liquid check valve it is provided with in equal liquid hole, all liquid check valves are in cell filtration net and rotate between pipe, all Tong Guo the direction of liquid check valve is by external to ware in ware body, liquid injection pipe lower end connects with portion in ware body.The invention has the beneficial effects as follows: be applicable to cell cultivate and measure, comparison, rationally and Stability Analysis of Structures, operating process is the most convenient, and the regulation concordance in process of the test is good, accuracy is high for space layout.
Description
Technical field
The invention belongs to technical field of cell culture, particularly relate to a kind of culture carrier structure.
Background technology
Cell is cultivated and is also cell clone technology, and the regular noun in biology is cell culture technology.The life of cell
Length needs nutrient environment, is referred to as culture medium for the nutrient matrix maintaining cell to grow.Culture medium can be divided into by its physical state
Fluid medium and solid medium.Cell culture technology, in addition to being applied to produce, is the most more for testing (as right
Group sample parameter be measured, and compare, analyze and study), and test in the more commonly used to be fluid medium.This
Outward, cell is cultivated to be needed to use cell culture system, common are the cell under culture dish, incubator etc., general environment and cultivates
Culture dish can be directly used, if desired for some special environments (such as energising, heating etc.), then special incubator can be used.
At present carry out cell cultivation and during Comparability test time, although common culture apparatus can play and carry for culture medium
For the function of bearing space, but at aspects such as space layout reasonability, integrated operation convenience, regulating effect concordance, still have
It is short of.
Summary of the invention
The present invention is to overcome deficiency of the prior art, it is provided that one is applicable to cell and cultivates, measures and analyze
Comparison, rationally and Stability Analysis of Structures, operating process is the most convenient, and the regulation concordance in process of the test is good, accuracy for space layout
High culture carrier structure.
To achieve these goals, the present invention is by the following technical solutions:
A kind of culture carrier structure, including base and the culture vessel of some upper end open, each culture vessel is the most successively
Stacking, a culture vessel contact base of bottom, described base is provided with fixing vertical rod, and culture vessel lateral wall is provided with
The retaining collar adaptive with fixing vertical rod, retaining collar is set in fixing vertical rod, and culture vessel includes ware body, is arranged on ware body
Column on inner bottom part and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, the equal opening of column upper/lower terminal, one is erected
The straight rotation pipe arranged can relatively rotate through all columns, described rotation pipe with column, and rotates in pipe lateral wall and column
It is in sealing contact between sidewall, rotates pipe lateral wall and be provided with the sump pit running through rotation pipe upper/lower terminal, a culture vessel
In: column is provided with the equal liquid hole connected with portion in ware body, is provided with in cell filtration net, liquid injection pipe lower end and ware body in equal liquid hole
Portion connects, and ware body sidewall is provided with the tune amount transverse tube connected with portion in ware body, and tune amount transverse tube is provided with and runs through tune amount transverse tube two
The tune amount post of pipe end, tune amount post is threadeded with tune amount transverse tube, and tune amount post is coaxial with tune amount transverse tube, and tune amount post the inner is in ware
In body, tune amount post outer end is in outside tune amount transverse tube, and tune amount post sidewall is provided with baseline trough and is in the alignment mark outside tune amount transverse tube
Show that bar, tune amount transverse tube are provided with baseline rod.
As preferably, described liquid injection pipe upper end is provided with and the lid of liquid injection pipe threaded engagement, rotate be arranged with on pipe for
The vessel cover that the upper end open of the top culture vessel is sealed.
As preferably, described base includes matrix and some support columns being arranged on below matrix, and matrix is provided with and runs through
The end through hole on the upper and lower surface of matrix, rotates pipe lower end and stretches in end through hole, is provided with and opening up connects fluid cylinder below end through hole,
Being provided with the bottom bracing plate being connected with at least one support column below matrix, bottom bracing plate is provided with and connects cylinder groove adaptive bottom fluid cylinder,
Connect and be in bottom fluid cylinder in a groove.
As preferably, being provided with core pipe and some horizontally disposed diaphragm plates in described rotation pipe, it is logical that the end, is stretched in core pipe lower end
In hole, core pipe being arranged with support ring, support ring is higher than bottom core pipe and support ring is in end through hole, is provided with and props up in end through hole
Support seat, supports seat top and is provided with seat slot and the annular groove adaptive with rotating pipe lower end, contact seat slot bottom land, rotate pipe bottom support ring
Contacting annular groove bottom land in annular groove is stretched in lower end and bottom rotation pipe, rotate pipe and be rotatably assorted with supporting seat, core pipe passes and supports seat
And core pipe with support seat be rotatably assorted, diaphragm plate is set on core pipe, and diaphragm plate cross section is annular, rotate pipe, core pipe and
Each diaphragm plate is all coaxial, and core pipe can relatively rotate with diaphragm plate, is in sealing contact between core pipe and diaphragm plate, and diaphragm plate is managed with rotating
Between seal fixing；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One is corresponding, in corresponding hole sealing plate with diaphragm plate: hole sealing plate is in above diaphragm plate, and contact diaphragm plate top, hole sealing plate bottom surface
Face；
Rotate and form quantitative chamber between the interior adjacent two pieces of diaphragm plates of pipe, if rotating pipe to be provided with main feeding hole, quantitative chamber and pipe
To fluid apertures one_to_one corresponding, quantitative chamber and culture vessel one_to_one corresponding, in corresponding quantitative chamber with culture vessel: be provided with on column
Fluid apertures given by cylinder, and quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe to fluid apertures and cylinder to fluid apertures all in cylinder,
And the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder feeding axially bored line level, quantitative chamber lowest part is higher than cylinder to fluid apertures
Lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
As preferably, in corresponding quantitative chamber with culture vessel: quantitatively intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule sets
Having for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end connects with tune amount liquid capsule, and feed pipe is by being in quantitative intracavity
Hard tube section and the outer segment being in outside quantitative chamber are constituted；
Base is provided with some for the confession fluid cylinder of feed pipe feed flow, including that cylinder body coordinates with cylinder body slipper seal for fluid cylinder
Feed flow piston and be connected and be in the piston rod above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, at one in fluid cylinder: in cylinder body, the space below feed flow piston is sap cavity, space above feed flow piston in cylinder body
For regulation chamber；
Sap cavity and feed pipe one_to_one corresponding, the feed pipe other end connects with corresponding sap cavity, feed pipe pass rotation tube side wall and
Rotate pipe lower end, seal fixing between feed pipe and rotation tube side wall.
As preferably, described base is provided with the adjustment seat fixing with base, is provided with horizontally disposed tune below adjustment seat
Joint plate, piston rod passes adjustable plate, and piston rod is provided with upper holder block and lower drawing block, and adjustment seat is provided with vertically arranges and through adjusting
Saving the homophony pole of seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, at the bottom of upper holder block
Face contact adjustable plate end face, lower drawing block end face contact adjustable plate bottom surface, main regulation masthead portion is provided with the master fixing with homophony pole
Adjusting knob.
As preferably, described homophony pole is provided with the instruction dish being in above adjustment seat, and instruction dish is same with homophony pole
Axle, adjustment seat is provided with the indicating arm of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction dish sidewall.
As preferably, in described rotation pipe, be provided with the sealing depression bar of vertically layout, seal depression bar through all diaphragm plates and
Seal depression bar all to threaded between all diaphragm plates, seal depression bar and be provided with some pressure strips for compressing hole sealing plate,
Pressure strip and hole sealing plate one_to_one corresponding, pressure strip is in the top of corresponding hole sealing plate.
The invention has the beneficial effects as follows: be applicable to cell cultivate and measure, comparison, space layout rationally and Stability Analysis of Structures,
Operating process is the most convenient, and the regulation concordance in process of the test is good, accuracy is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation at tune amount transverse tube of the present invention；
Fig. 3 is the structural representation of orifice plate of the present invention；
Fig. 4 is the enlarged drawing in Fig. 1 at A；
Fig. 5 is the enlarged drawing in Fig. 1 at B；
Fig. 6 is the enlarged drawing in Fig. 1 at C；
Fig. 7 is the sectional view that the present invention rotates at pipe.
In figure: base 1, fixing vertical rod 11, connect fluid cylinder 12, bottom bracing plate 13, support seat 14, matrix 1a, support column 1b, the end, lead to
Hole 1c, culture vessel 2, retaining collar 21, equal liquid hole 22, lid 23, vessel cover 24, orifice plate 25, cylinder are to fluid apertures 26, tune amount transverse tube
271, tune amount post 272, alignment mark rod 28, baseline rod 29, ware body 2a, column 2b, liquid injection pipe 2c, rotate pipe 3, sump pit 31,
Diaphragm plate 32, even lumen pore 321, pipe are to fluid apertures 33, tune amount liquid capsule 34, quantitative chamber 3a, core pipe 4, support ring 41, hole sealing plate 42, confession
Fluid cylinder 5, cylinder body 5a, feed flow piston 5b, piston rod 5c, sap cavity 5d, regulation chamber 5e, adjustment seat 6, adjustable plate 61, upper holder block 611,
Lower drawing block 612, homophony pole 62, main regulation knob 621, instruction dish 622, indicating arm 63, sealing depression bar 7, pressure strip 71.
Detailed description of the invention
The present invention will be further described with detailed description of the invention below in conjunction with the accompanying drawings.
In embodiment as shown in Figures 1 to 7, a kind of culture carrier structure, including base 1 and the training of some upper end open
Supporting vessel 2, each culture vessel stacks gradually from the bottom to top, a culture vessel contact base of bottom, and described base sets
Having fixing vertical rod 11, culture vessel lateral wall is provided with and fixes the retaining collar 21 that vertical rod is adaptive, and retaining collar is set in solid
Determining in vertical rod, culture vessel includes ware body 2a, the column 2b being arranged on the interior bottom of ware body and is arranged on ware body sidewall and inclines
The liquid injection pipe 2c tiltedly arranged, the equal opening of column upper/lower terminal, the rotation pipe 3 of a vertical layout through all columns, described turn
Dynamic pipe can relatively rotate with column, and is in sealing contact between rotation pipe lateral wall and column medial wall, rotates and sets on pipe lateral wall
Have and run through the sump pit 31 rotating pipe upper/lower terminal, in a culture vessel: be provided with equal with what portion in ware body connected on column
Fluid apertures 22, is provided with cell filtration net in equal liquid hole, liquid injection pipe lower end connects with portion in ware body, ware body sidewall be provided with in ware body
The tune amount transverse tube 271 of portion's connection, tune amount transverse tube is provided with the tune amount post 272 running through tune two pipe ends of amount transverse tube, tune amount post and tune
Amount transverse tube is threaded, and tune amount post is coaxial with tune amount transverse tube, and tune amount post the inner is in ware body, and tune amount post outer end is in tune amount
Outside transverse tube, tune amount post sidewall is provided with baseline trough and is in the alignment mark rod 28 outside tune amount transverse tube, and tune amount transverse tube is provided with base
Quasi-bar 29.
Before test, each culture vessel can be stacked gradually placement from the bottom to top, and it is fixing vertical to make retaining collar be inserted in when placing
Bar, make column set to rotating pipe outer (rotate pipe and the structure that rotates in pipe can pre-set) so that each culture vessel
Can align, the culture vessel of top just covers the culture vessel of lower section.Then each liquid injection pipe is carried out liquid feeding and (adds liquid
Body culture medium or culture fluid), without deliberately controlling liquid volume added during liquid feeding, as long as liquid level lid crosses equal liquid hole.All liquid feeding is complete
Cheng Hou, turn rotates pipe, allows sump pit connect with each equal liquid hole, so that the liquid in each culture vessel can be from equal liquid hole stream
Going out, in culture vessel, remaining liquid measure reaches consistent, turn can rotate pipe subsequently, allow sump pit leave equal liquid hole and (make it not connect
Logical), the most just it is rapidly completed liquid feeding.And want to regulate in ware body if liquid measure, it is also possible to realized by turn tune amount post,
And mode is the most various: as, before liquid feeding, tune can be measured post inwardly (outward) turn certain distance, the most whole liquid feeding walks
Suddenly the liquid measure finally stayed in ware body (after the process utilizing equal liquid hole and sump pit to carry out discharge opeing terminates) after completing can reduce and (increase
Add)；And for example, after liquid feeding, sump pit is when connecting with equal liquid hole, tune can be measured post inside turn certain distance, continue to discharge
A part of liquid in ware body, thus allow the liquid measure stayed in ware body reduce.In above-mentioned regulative mode, the latter is more commonly used, this
It is because after once liquid feeding completes, is relatively time-consuming with the mode discharge opeing again drawn, topple over, and operation is the most pretty troublesome, but
Discharge opeing, regulation is carried out by aforementioned manner, the most very convenient.And the fixing quantity mode of tune amount post displacement is the most
Sample, gives one example: in the present embodiment, at the beginning, can allow alignment mark rod align with baseline rod, baseline trough remote with tune amount transverse tube
Align from one end of ware body, can be set as reference position herein and (baseline trough can also be allowed to be aligned in other setting position, depending on making
Depending on the considering when demand of used time, design), after liquid feeding, sump pit is when connecting with equal liquid hole, the often inside turn one of tune amount post
Circle, alignment mark rod will turn one circle again align with baseline rod that (mark rod that aligns, the baseline rod person of being convenient to operation are carried out
Observe and regulation), due to tune amount post inside turn one enclose after, stretch into the volume in ware body be definite value (this value design time just
Have determined that), so the liquid measure discharged also is definite value.During additionally, cultivate, test, tend not to be that all tune fluid columns are all used
Arrive, or each adjust fluid column turn distance can difference, this be in order to adapt to different sample (variety classes cell may needs not
Same initial incubation liquid measure) or quickly provide different culture environment (to come with the identical or different cultivation liquid measure of cell types
Carry out cultivating and subsequent measurements, Comparability test) and the structure that designs.It is noted that alignment mark rod is preferably provided at ware body
Outward, because being once arranged in ware body, alignment mark rod easily produces impact to liquid level when rotating, and degree of regulation can be by certain
Impact, and it is unfavorable for that observation (if culture vessel is opaque, then can cause not observing, if culture vessel is transparent, see
Examine also not as align mark rod be arranged on ware external time convenient).
It is pointed out that cell to be cultivated can just be inserted culture vessel before adding culture fluid and (be needed to wait to cultivate
Cell of short duration can leave culture fluid and non-inactivation or death), now owing to there being the existence of cell filtration net, cell will not flow out
Culture vessel；Can also add at culture fluid, equal hydraulic control amount (i.e. carries out discharge opeing mentioned by equal liquid hole so that liquid in each ware body
Measure consistent process) add cell to be cultivated afterwards；The culture fluid that band also can directly need to be cultivated cell is directly injected into training
(test under this premise should not consider the impact of initial cell quantity, and owing to having the existence of cell filtration net, cell to support vessel
Will not flow out culture vessel).Specifically can select according to test objective, demand.
Described liquid injection pipe upper end is provided with the lid 23 with liquid injection pipe threaded engagement, rotates and is arranged with for by the top on pipe
The vessel cover 24 that seals of the upper end open of a culture vessel.Lid can block and rotate pipe and extraneous connection, vessel cover
Can block a culture vessel of the top and extraneous connection, remaining culture vessel is then by a training nearest above self
Support vessel to block and extraneous connection.Such that it is able to ensure that each culture vessel separates with the external world in cell cultivation, process of the test,
Thus allow the present invention be applicable to the test being hedged off from the outer world under premise.
Described base includes matrix 1a and some support column 1b being arranged on below matrix, and matrix is provided with and runs through matrix
The end through hole 1c on upper and lower surface, rotates pipe lower end and stretches in end through hole, is provided with and opening up connects fluid cylinder 12 below end through hole,
Being provided with the bottom bracing plate 13 being connected with at least one support column below matrix, bottom bracing plate is provided with and connects cylinder adaptive bottom fluid cylinder
Groove, connects and is in bottom fluid cylinder in a groove.The liquid discharged from sump pit can be reclaimed (fall into and connect fluid cylinder) easily, can avoid wave
Take and avoid polluting to external world.
Described column includes cylinder and orifice plate 25, and cylinder lateral wall is provided with side opening, on a column: orifice plate is stuck in side opening
In, it being in sealing contact between orifice plate and side opening hole wall, orifice plate is made up of with lower half plate mutually symmetrical with upper half plate, upper half plate and lower half
Plate is formed in one structure, and equal liquid hole is positioned on upper half plate.In addition to equal liquid hole, orifice plate is laterally zygomorphic structure, consequently, it is possible to
One piece of orifice plate can be achieved with the Liquid level of two kinds of differing heights, i.e. by orifice plate with upper half plate upper and lower half plate under form
Snap in side opening, then through equal hydraulic control amount (i.e. carry out discharge opeing mentioned by equal liquid hole so that process that in each ware body, liquid measure is consistent)
After, liquid level consistent with equal liquid hole lowest part (now equal liquid hole is in more than in the middle part of orifice plate) in culture vessel；And by orifice plate
With upper half plate under, lower half plate snap in side opening in upper form, then liquid level and equal liquid hole lowest part in final culture vessel
Unanimously (now equal liquid hole is in the middle part of orifice plate following).Furthermore, it is possible to orifice plate is overlapped in configuration more, between each set orifice plate, only has equal liquid hole
Highly different, in so can be achieved with culture vessel, which liquid level is the quick control of the initial liquid measure of various differences (need, just
Use in a set of orifice plate corresponding with this liquid level is snapped in advance the side opening of each culture vessel).
Being provided with core pipe 4 and some horizontally disposed diaphragm plates 32 in described rotation pipe, core pipe lower end is stretched in end through hole, core
Being arranged with support ring 41 on pipe, support ring is higher than bottom core pipe and support ring is in end through hole, is provided with support seat in end through hole
14, support seat top and be provided with seat slot and the annular groove adaptive with rotating pipe lower end, contact seat slot bottom land bottom support ring, rotate under pipe
End stretches in annular groove and rotates and contacts annular groove bottom land bottom pipe, rotates pipe and is rotatably assorted with supporting seat, core pipe through support seat and
Core pipe is rotatably assorted with supporting seat, and diaphragm plate is set on core pipe, and diaphragm plate cross section is annular, rotates pipe, core pipe and each
Diaphragm plate is all coaxial, and core pipe can relatively rotate with diaphragm plate, is in sealing contact between core pipe and diaphragm plate, diaphragm plate and rotation pipe
Between seal fixing；
Diaphragm plate is provided with even lumen pore 321, and core pipe is provided with some hole sealing plates 42 for the company's of sealing lumen pore, hole sealing plate and tabula
Plate one_to_one corresponding, in corresponding hole sealing plate with diaphragm plate: hole sealing plate is in above diaphragm plate, hole sealing plate bottom surface contact diaphragm plate
End face；
Rotate and form quantitative chamber 3a between the interior adjacent two pieces of diaphragm plates of pipe, if rotating pipe to be provided with main feeding hole 33, quantitative chamber
With pipe to fluid apertures one_to_one corresponding, quantitative chamber and culture vessel one_to_one corresponding, in corresponding quantitative chamber with culture vessel: on column
Being provided with cylinder to fluid apertures 26, quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe to fluid apertures and cylinder to fluid apertures all in
Cylinder, and the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder feeding axially bored line level, quantitative chamber lowest part is higher than cylinder
To fluid apertures lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
When needing to carry out each culture vessel mid-term while, liquid feeding (adds new culture fluid, Ke Yiyu in incubation
Initial incubation liquid kind, proportioning are identical, it is also possible to different from initial incubation liquid kind, proportioning) time, for each ware body, liquid
The time difference that body adds is the least, and exopathogenic factor interference suffered during final mensuration is the least (to be preferably capable of being simultaneously introduced cultivation
Liquid).But unless allow many people simultaneously to each culture vessel to carry out property while liquid feeding, and height to be ensured, can be only achieved more
Preferably effect, and in practical operation, the feasibility of this mode is on the low side.And in this programme, first turn rotates pipe, allow pipe
To fluid apertures with corresponding cylinder to stagger between fluid apertures (not connecting), then allow rotation pipe motionless, first turn core pipe (if rotate pipe with
Between column, frictional force is sufficiently large, then can direct turn core pipe, now rotating pipe will not be driven；Rub between pipe and column if rotating
Wiping power is limited, then can pinch rotation pipe on the other hand, another hands carrys out turn core pipe；The present invention design time, preferential former feelings
Shape), drive hole sealing plate leave even lumen pore (allow shrouding not company of sealing lumen pore), then can carry out liquid feeding from rotating pipe top, cultivation
Liquid through each even lumen pore, and can finally be full of all of quantitative chamber, then proceedes to make rotation pipe motionless, and turn core pipe makes envelope
Orifice plate seals even lumen pore, cuts off, and each quantitative intracavity liquid measure is consistent between the most each quantitative chamber.Then turn pipe can be rotated, logical
Cross diaphragm plate drive core pipe rotate together (diaphragm plate and to rotate between pipe be fixing, although can phase between core pipe and diaphragm plate
To rotation, but owing to being in sealing contact between core pipe and diaphragm plate, therefore between core pipe and diaphragm plate, necessarily there is the power of compression mutually
And frictional force, so if relatively rotating, need applying allow they external force in relative rotation.And only turn core pipe time, rubbing
Under the effect of wiping power, diaphragm plate can rotate together with core pipe, so rotating relative invariant position between Guan Yuxin pipe), allow
Pipe connects between fluid apertures to fluid apertures and corresponding cylinder, the most each quantitative chamber can give in corresponding ware body portion simultaneously, equivalent
Liquid feeding, can thoroughly eliminate the interference factors such as the liquid feeding time started is inconsistent, liquid feeding duration is inconsistent during middle liquid feeding.This
Outward, this programme except can be used for liquid feeding in mid-term it can also be used to initially time liquid feeding.And after having had support seat, arrange from sump pit
The liquid gone out can first reach to support seat top, the most outwards flows down.
During it is noted that sump pit connects with equal liquid hole, pipe staggers between fluid apertures with corresponding cylinder to fluid apertures；Pipe is given
Fluid apertures is with corresponding cylinder to when connecting between fluid apertures, and sump pit staggers with equal liquid hole.And through equal hydraulic control amount (i.e. mentioned by all
Fluid apertures carries out discharge opeing so that process that in each ware body, liquid measure is consistent) after, in utilizing quantitative chamber to ware body, carry out the behaviour of liquid feeding
During work (when turn rotates pipe), it is impossible to allow sump pit again connect with equal liquid hole, otherwise can cause leakage at sump pit
(in ware body, liquid flows out from equal liquid hole, sump pit again), specific constructive form and mode of operation have a variety of, this measure two
Example,
Example 1: in the circumference rotating pipe, sump pit, equal liquid hole, pipe are arranged along up time pointing needle to fluid apertures successively to fluid apertures, cylinder.
All during hydraulic control amount (i.e. carry out discharge opeing mentioned by equal liquid hole so that process that in each ware body, liquid measure is consistent) operation, sump pit with
Rotation pipe and be clockwise to the equal liquid hole (during Gai, pipe connect to fluid apertures with corresponding cylinder to fluid apertures) that aligns, equal hydraulic control amount
After completing, sump pit continues to rotate clockwise and stagger with equal liquid hole, and now pipe does not still have to fluid apertures with corresponding cylinder to fluid apertures
Reaching connected state, if continuing turn clockwise subsequently to rotate pipe, pipe can be allowed to connect to fluid apertures with corresponding cylinder to fluid apertures；
Example 2: in the circumference rotating pipe, equal liquid hole, sump pit, pipe are arranged to fluid apertures the most successively to fluid apertures, cylinder,
All during hydraulic control amount (i.e. carry out discharge opeing mentioned by equal liquid hole so that process that in each ware body, liquid measure is consistent) operation, sump pit with
Rotation pipe to rotate counterclockwise to alignment equal liquid hole (during Gai, pipe connect to fluid apertures with corresponding cylinder to fluid apertures), equal hydraulic control amount
After completing, sump pit rotates clockwise and staggers with equal liquid hole, and now pipe is still not reaching to fluid apertures with corresponding cylinder to fluid apertures
Connected state, if continuing turn clockwise subsequently to rotate pipe, can allow pipe connect to fluid apertures with corresponding cylinder to fluid apertures.
In corresponding quantitative chamber with culture vessel: quantitatively intracavity is provided with tune amount liquid capsule 34, tune amount liquid capsule be provided with for
To the feed pipe of tune amount liquid capsule feed flow, feed pipe one end connects with tune amount liquid capsule, and feed pipe is by the hard tube section being in quantitative intracavity
And the outer segment being in outside quantitative chamber is constituted；
Base is provided with some for the confession fluid cylinder 5 of feed pipe feed flow, including that cylinder body 5a joins with cylinder body slipper seal for fluid cylinder
The feed flow piston 5b that closes and be connected with feed flow piston and be in the piston rod 5c above feed flow piston, feed flow piston slidably just
To for above-below direction, in one for fluid cylinder: in cylinder body, the space below feed flow piston is sap cavity 5d, in cylinder body on feed flow piston
The space of side is regulation chamber 5e；
Sap cavity and feed pipe one_to_one corresponding, the feed pipe other end connects with corresponding sap cavity, feed pipe pass rotation tube side wall and
Rotate pipe lower end, seal fixing between feed pipe and rotation tube side wall.
Described base is provided with the adjustment seat 6 fixing with base, is provided with horizontally disposed adjustable plate 61 below adjustment seat, lives
Stopper rod passes adjustable plate, and piston rod is provided with upper holder block 611 and lower drawing block 612, and adjustment seat is provided with vertically arranges and through adjusting
Saving the homophony pole 62 of seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, upper holder block
Bottom surface contact adjustable plate end face, lower drawing block end face contact adjustable plate bottom surface, main regulation masthead portion is provided with fixing with homophony pole
Main regulation knob 621.
Described homophony pole is provided with the instruction dish 622 being in above adjustment seat, and instruction dish is coaxial with homophony pole, regulation
Seat is provided with the indicating arm 63 of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction dish sidewall.
As it was previously stated, the present invention can carry out equivalent, simultaneously liquid feeding in each ware body.And in this programme, it is also possible to right
Liquid volume added regulates and controls.At the beginning, tune amount liquid capsule is vacuum, if now specified rate chamber is full of, then liquid volume added is exactly the reality in quantitative chamber
Border volume.When needs control liquid volume added, homophony pole can be made to move up or down with turn homophony pole (main regulation knob)
Dynamic, as a example by moving down: now homophony pole drives adjustable plate vertically to move down (upper holder block by the upper holder block on homophony pole
Rotating, adjustable plate does not turns), adjustable plate pushes down on each feed flow piston, the liquid in each sap cavity by each lower drawing block on piston rod
Body (can be water or other liquid, if in stable condition, the structures such as the pipeline of process will not be damaged) inject right
The tune amount liquid capsule answered, now, the actual volume (actual volume refers to accommodate the volume of culture fluid) in quantitative chamber diminishes,
And the numerical value specifically diminished, can read from indicating arm (the scale groove on indicating arm can mark actual with quantitative chamber hold
Long-pending corresponding numeral, the occurrence of numeral, when manufacturing and designing, can convert with measured in advance.Bid can be done on instruction dish
Note, it is simple to directly carry out, according to labelling, the reading that mark-sense is alignd, or, instruction dish can also make as labelling itself
With, as utilized instruction dish end face or bottom surface to be used as labelling).The actual volume in quantitative chamber can be diminished by said process, thus
Control liquid volume added (making it reduce)；Otherwise, allow homophony pole move up, then tune can be measured the liquid in liquid capsule and extract out, thus
Liquid volume added (making it increase) can be controlled.It is noted that feed pipe is by being in the hard tube section of quantitative intracavity and being in outside quantitative chamber
Outer segment constitute, wherein outer segment can not be perfect rigidity, to guarantee that diaphragm plate, core pipe etc. can normal rotation (core pipes
Being connected with outer segment, when rotating core pipe, diaphragm plate, outer segment is also required for certain locomotivity), outer segment can
To select the pipe of the type such as sebific duct, oil cylinder petroleum pipeline.
It is provided with the sealing depression bar 7 of vertically layout in described rotation pipe, seals depression bar and through all diaphragm plates and seal depression bar
All threaded with between all diaphragm plates, seal depression bar and be provided with some pressure strips 71 for compressing hole sealing plate, pressure strip
With hole sealing plate one_to_one corresponding, pressure strip is in the top of corresponding hole sealing plate.As it was previously stated, fill it up with liquid in rotation pipe, then seal
Orifice plate seals after connecting lumen pore, cuts off between each quantitative chamber.But, in order to allow hole sealing plate want can the thoroughly company's of sealing lumen pore, hole sealing plate
And the extruding mutually between one piece of diaphragm plate of lower contact is inevitable bigger.After being used for multiple times, easily cause because abrasion is relatively big
Seal deficiency.And in this programme, hole sealing plate seals after connecting lumen pore, it is also possible to turn seals depression bar, pressure strip is driven to move down and press
Tight corresponding hole sealing plate, improves sealing further, ensures the partition effect between each quantitative chamber.And had sealing depression bar, pressure
After tight plate, during fabrication, need not press between hole sealing plate and diaphragm plate too tight (sealing can be protected by the pressure down of pressure strip
Barrier), such that it is able to effectively reduce abrasion, increase the service life.It is noted that after having had sealing depression bar, pressure strip, turn allowing
Dynamic pipe is motionless and before turn core pipe, pressure strip should be allowed to be not located at compressing the state of corresponding hole sealing plate, otherwise there will be " turning motionless "
Situation.
Claims (7)
1. a culture carrier structure, is characterized in that, including base and the culture vessel of some upper end open, each culture vessel by
Under supreme stack gradually, a culture vessel contact base of bottom, described base is provided with fixing vertical rod, outside culture vessel
Sidewall is provided with and fixes the retaining collar that vertical rod is adaptive, and retaining collar is set in fixing vertical rod, culture vessel include ware body,
Being arranged on the column on the interior bottom of ware body and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, column upper/lower terminal is equal
Opening, the rotation pipe of a vertical layout can relatively rotate through all columns, described rotation pipe with column, and rotates outside pipe
It is in sealing contact between wall and column medial wall, rotates pipe lateral wall and be provided with the sump pit running through rotation pipe upper/lower terminal, one
In individual culture vessel: column is provided with the equal liquid hole connected with portion in ware body, is provided with cell filtration net, under liquid injection pipe in equal liquid hole
End connects with portion in ware body, and ware body sidewall is provided with the tune amount transverse tube connected with portion in ware body, and tune amount transverse tube is provided with and runs through tune
The tune amount post of amount two pipe ends of transverse tube, tune amount post is threadeded with tune amount transverse tube, and tune amount post is coaxial with tune amount transverse tube, tune amount post
The inner is in ware body, and tune amount post outer end is in outside tune amount transverse tube, and tune amount post sidewall is provided with baseline trough and is in tune amount transverse tube
Outer alignment mark rod, tune amount transverse tube is provided with baseline rod.
A kind of culture carrier structure the most according to claim 1, is characterized in that, described base includes matrix and some settings
Support column below matrix, matrix is provided with the end through hole running through the upper and lower surface of matrix, rotates pipe lower end and stretches into end through hole
In, it is provided with the opening up fluid cylinder that connects below end through hole, below matrix, is provided with the bottom bracing plate being connected with at least one support column, the end
Fagging is provided with and connects cylinder groove adaptive bottom fluid cylinder, connects and is in bottom fluid cylinder in a groove.
A kind of culture carrier structure the most according to claim 1, is characterized in that, is provided with core pipe and some in described rotation pipe
Horizontally disposed diaphragm plate, core pipe lower end is stretched in end through hole, and core pipe is arranged with support ring, support ring higher than bottom core pipe and
Support ring is in end through hole, is provided with support seat in end through hole, supports seat top and is provided with seat slot and adaptive with rotating pipe lower end
Annular groove, contacts seat slot bottom land bottom support ring, rotate in annular groove is stretched in pipe lower end and rotate contact annular groove bottom land bottom pipe, rotating
Managing and be rotatably assorted with support seat, core pipe is rotatably assorted through supporting seat and core pipe with support seat, and diaphragm plate is set on core pipe, horizontal
Dividing plate cross section is annular, rotates pipe, core pipe and each diaphragm plate all coaxial, and core pipe can relatively rotate with diaphragm plate, core pipe and
It is in sealing contact between diaphragm plate, seals fixing between diaphragm plate and rotation pipe；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One is corresponding, in corresponding hole sealing plate with diaphragm plate: hole sealing plate is in above diaphragm plate, and contact diaphragm plate top, hole sealing plate bottom surface
Face；
Rotate and form quantitative chamber between the interior adjacent two pieces of diaphragm plates of pipe, if rotating pipe to be provided with main feeding hole, quantitative chamber and pipe
To fluid apertures one_to_one corresponding, quantitative chamber and culture vessel one_to_one corresponding, in corresponding quantitative chamber with culture vessel: be provided with on column
Fluid apertures given by cylinder, and quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe to fluid apertures and cylinder to fluid apertures all in cylinder,
And the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder feeding axially bored line level, quantitative chamber lowest part is higher than cylinder to fluid apertures
Lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
A kind of culture carrier structure the most according to claim 3, is characterized in that, in corresponding quantitative chamber and culture vessel
In: quantitatively intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule is provided with for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end with
Tune amount liquid capsule connects, and feed pipe is made up of the hard tube section being in quantitative intracavity and the outer segment that is in outside quantitative chamber；
Base is provided with some for the confession fluid cylinder of feed pipe feed flow, including that cylinder body coordinates with cylinder body slipper seal for fluid cylinder
Feed flow piston and be connected and be in the piston rod above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, at one in fluid cylinder: in cylinder body, the space below feed flow piston is sap cavity, space above feed flow piston in cylinder body
For regulation chamber；
Sap cavity and feed pipe one_to_one corresponding, the feed pipe other end connects with corresponding sap cavity, feed pipe pass rotation tube side wall and
Rotate pipe lower end, seal fixing between feed pipe and rotation tube side wall.
A kind of culture carrier structure the most according to claim 4, is characterized in that, described base is provided with fixing with base
Adjustment seat, is provided with horizontally disposed adjustable plate below adjustment seat, piston rod pass adjustable plate, piston rod be provided with upper holder block and under
Pulling block, adjustment seat is provided with the homophony pole vertically arranging and passing adjustment seat, and homophony pole is threadeded with adjustment seat, homophony
Pole is provided with upper holder block and lower drawing block, upper holder block bottom surface contact adjustable plate end face, lower drawing block end face contact adjustable plate bottom surface, master
Adjusting rod top is provided with the main regulation knob fixing with homophony pole.
A kind of culture carrier structure the most according to claim 5, is characterized in that, described homophony pole is provided with and is in regulation
Instruction dish above Zuo, instruction dish is coaxial with homophony pole, and adjustment seat is provided with the indicating arm of groove with a scale, the vertical cloth of indicating arm
Put and indicating arm contact instruction dish sidewall.
7. according to a kind of culture carrier structure described in claim 3 or 4 or 5, it is characterized in that, be provided with in described rotation pipe vertically
The sealing depression bar arranged, seals depression bar and all threadeds with all diaphragm plates through all diaphragm plates and sealing depression bar, close
Envelope depression bar is provided with some pressure strips for compressing hole sealing plate, pressure strip and hole sealing plate one_to_one corresponding, and pressure strip is in correspondence
The top of hole sealing plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610699550.6A CN106119110A (en) | 2016-08-22 | 2016-08-22 | A kind of culture carrier structure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610699550.6A CN106119110A (en) | 2016-08-22 | 2016-08-22 | A kind of culture carrier structure |
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| CN106119110A true CN106119110A (en) | 2016-11-16 |
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| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106190843A (en) * | 2016-08-22 | 2016-12-07 | 王铁苗 | A kind of multisample culture apparatus |
| CN106222089A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test auxiliary culture apparatus |
| CN106222090A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test assistant carrier |
| CN106222087A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of sample culture carrier |
| CN106244457A (en) * | 2016-08-22 | 2016-12-21 | 王铁苗 | Culture carrier structure is used in a kind of test |
| CN106282017A (en) * | 2016-08-22 | 2017-01-04 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106467893A (en) * | 2016-08-22 | 2017-03-01 | 王铁苗 | A kind of experiment auxiliary device |
| CN113713434A (en) * | 2021-08-12 | 2021-11-30 | 中国人民解放军陆军军医大学第一附属医院 | Blood platelet separator |
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| CN113713434A (en) * | 2021-08-12 | 2021-11-30 | 中国人民解放军陆军军医大学第一附属医院 | Blood platelet separator |
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Application publication date: 20161116 |