CN105967840A - A microbial fertilizer promoting root nodule growth for soybean and a preparing method thereof - Google Patents

Abstract

A microbial fertilizer promoting root nodule growth for soybean and a preparing method thereof are disclosed. The fertilizer is prepared from papermaking black liquid, a sulfuric solution having a concentration of 10 wt%, a formaldehyde solution having a concentration of 5%, sodium sulfite, bone meal, brown sugar residues, ardealite, azotobacteria, potassium bacteria, phosphate solubilizing bacteria, edible mushroom leftovers, red bean residues, Chinese milk vetch straw, calcium ammonium nitrate, ammonium molybdate, ferrous sulfate, zinc chloride, shell powder and a proper amount of water by weight parts. The fertilizer is an organic-inorganic composite fertilizer rich in microbes. Soil microbe inhibiting functions of inorganic components after being adsorbed by lignin can be greatly reduced so that the living environment of the azotobacteria and other microbes added is good. Through simple multiplication culture and adaptive culture, viability of microbes in soil is greatly improved. Soybean root nodule formation is effectively promoted. Absorption of potassium and phosphorus is promoted. Disease resistance of leguminous plants is improved.

Description

A kind of Semen sojae atricolor promotes microorganism fertilizer of Nodule Growth and preparation method thereof

Technical field

The invention belongs to the Technology field of fertilizer, be specifically related to a kind of Semen sojae atricolor microorganism fertilizer promoting Nodule Growth and preparation method thereof.

Background technology

The soda pulp technique that paper industry is widely used, no matter with woody or nonwood plant as raw material, all substantial amounts of black liquor of by-product, the annual black liquor yield in the whole world is about 500,000,000 tons according to statistics, the solid content that there are about 70% in black liquor is Organic substance, and including lignin, hemicellulose and cellulose degradation product etc., lignin is a kind of complicated macromolecular compound being present in major part land plant xylem, quantity is the hugest, estimates that the domestic lignin produced by papermaking every year there are about more than 1,000 ten thousand tons.At present, major part black liquor concentrated to 45 ~ 60% after-combustions, carry out alkali collection, thus causing the huge waste of this Renewable resource of lignin contained therein, black liquid flows into environment, and pollution level is high, containing substantial amounts of organic fiber, inorganic salt and pigment, contained pollutant have accounted for more than the 90% of industrial pollution caused by paper manufacturing thing, and alkalescence is relatively big, color is deep, and stink weight and foam are many, a large amount of dissolved oxygen consumed in water, cause serious destruction to environment.

The sulfonation of black liquid refers to that contained lignin reaction generates the process of lignosulfonate to sulfite ion with black liquor.The methylolation of first paragraph lignin, determines optimal value according to the introduction volume of methylol in lignin；The sulfonation of second segment lignin, determines optimal value according to paste flowing degree, is aided with sulfonation degree and relative molecular mass analysis.The method that wherein methylolation of lignin is to increase lignin activity；Sulfonating reaction is set as pH value≤7, makees sulfonating agent with sodium sulfite, and the lignin structure unit of phenolic and non-phenolic can occur the sulfonating reaction on beneficially α carbon, the most also carries out on γ carbon, but seldom on β carbon.

Black liquid in commercial paper is carried out emphasis utilization, it is subsequently used for the preparation of fertilizer, not only solve black liquid and flow into the environmental pollution that environment causes, also carry out garbage huge profit use, development for slow release fertilizer, the black liquid of main flow is with after caustic lye of soda high temperature steaming slurrying mostly now, originally lignin dissolution becomes alkali lignin, cellulose separates out, the black liquid obtained after leaching cellulose, so general pH is more than 12, total alkali 20 ~ 25%, lignin is 20 ~ 25g/L, and pollution level is high, containing a large amount of fibers, inorganic salt and pigment.

Summary of the invention

It is an object of the invention to huge profit black liquid, for the production of agrochemical, make a kind of granule adsorbing inorganic elements, make fertilizer play the effect of slow release, realize particular by following methods:

A kind of Semen sojae atricolor promotes the microorganism fertilizer of Nodule Growth, it is characterized in that, be made up of the raw material of following weight portion: black liquid 150 ~ 180,10wt% sulfuric acid solution 30 ~ 40,5% formalin 20 ~ 30, sodium sulfite 10 ~ 15, bone meal 10 ~ 14, brown sugar slag 15 ~ 20, ardealite 7 ~ 10, nitrogen-fixing bacteria 1 ~ 2, potassium solubilizing bacteria 0.5 ~ 0.8, phosphate solubilizing bacteria 0.2 ~ 0.5, edible fungi leftovers 26 ~ 30, Semen Phaseoli slag 10 ~ 14, Herba Astragali Melilotoidis (Herba Astragali Sinici) straw 16 ~ 22, calcium ammonium nitrate 10 ~ 13, ammonium molybdate 2 ~ 4, ferrous sulfate 6 ~ 10, zinc chloride 4 ~ 7, conch meal 5 ~ 8 and appropriate water.

A kind of Semen sojae atricolor promotes the preparation method of microorganism fertilizer of Nodule Growth, it is characterised in that include following step:

(1) black liquid is joined in reactor, it is heated to boiling, steaming and decocting is concentrated into original 1/3, then heating in water bath is turned to 50 ~ 60 DEG C, insulation, it is slowly stirred 30 ~ 40min with the rotating speed of 120 ~ 150r/min, it is simultaneously introduced 10wt% sulfuric acid solution, sucking filtration obtains lignin particles, crosses clear water and washs 3 ~ 5 times, finally standby after vacuum drying；

(2) lignin particles of (1) gained is joined in the container filling 5% formalin, regulation pH is 10 ~ 11, it is heated to 70 ~ 80 DEG C of insulation reaction 1.2 ~ 1.5h, pH value determination is addition sodium sulfite after neutrality, improve and at temperature 130 ~ 150 DEG C, react 3 ~ 4h, again wash and be dried, obtaining modified lignin particles；

(3) mixed edible bacterium leftover bits and pieces, Semen Phaseoli slag, Herba Astragali Melilotoidis (Herba Astragali Sinici) straw and other following residual components being not directed to, add the water of 2 ~ 3 times of its weight portions, water temperature 40 ~ 50 DEG C is soaked 2 ~ 3 days, then filter excessive moisture and pulverize slagging, spray the nitrogen-fixing bacteria of 10 times of use water dilution, potassium solubilizing bacteria, phosphate solubilizing bacteria mixture, heap fermentation 6 ~ 10 angel's Organic substance covers with mycelia thing；

(4) mixing ammonium nitrate calcium, ammonium molybdate, ferrous sulfate and zinc chloride, adds water in the ratio of material-water ratio 1:3 ~ 4, heated and stirred the lignin particles obtained by adding (2), and standing adsorption carries out ion exchange process, continues 20 ~ 30min；

(5) half organic fermentation materials of mixing (3) and the mixing and absorption inorganic nutrient solution described in (4), be watered holding water content 60 ~ 70%, slurry-spraying pelletizing in right amount.

The thinking of the present invention is by acid adding in the black liquid of alkalescence preparation method, the phenates being originally dissolved in alkali is made to make alkali lignin separate out with acid effect, first take a certain amount of black liquid, heating concentrates, the sulfuric acid solution being subsequently adding 10wt% makes the pH of concentrated solution be 2 ~ 3, allows lignin free adsorbing conglomeration form granule in the case of being slowly stirred, and controls mixing speed and makes granule be between 80 ~ 100 mesh sieves, then it is precipitated by the way of sucking filtration, is washed with deionized the lignin particles obtaining flocculating sedimentation 3 ~ 5 times；

Containing various active group in lignin molecule own, humus it is converted into after slowly being degraded by microorganism in soil, and humus is the main component of earth, extremely critical effect is played in growth to plant, soil urease liveness is had certain inhibitory action, promote plant growing, improvement soil etc..But can not single add in fertilizer as organic, organic offer nutrition is provided like that, can not produce with modern fertilizer and contact, and large usage quantity, effect is general, also it is unworthy expending material resources, the manpower transformation to black liquid, does not also meet the consumption that modern fertilizer advocates few, the obvious feature of effect；nullIn view of this point，Lignin is combined granule and carries out chemical modification by us，Make lignosulfonates，It is a kind of good raw material manufacturing ion exchange resin，Become a kind of macromolecular material，Lignin particles is owing to belonging to free settling，Do not add any binding agent，It is cross-linked to each other loosely organized，After making lignosulfonates，Belong to macromolecular structure，Be cross-linked to each other more horn of plenty，It is not easily broken，There is the strongest cation exch ange adsorption ability simultaneously，And the shortcoming overcoming the poorly water-soluble of lignin own，Invention be not intended to prepare pure cation exchange resin，Mainly controlled by certain condition，The combination making lignin particles is tightr，Strengthen its hydrophilic，The lignin molecule simultaneously making particle surface is converted into lignosulfonates，For adsorbing metal cation，After adding fertilizer，Play certain slow release effect.

Beneficial effects of the present invention: this is the fertilizer of the organic composite inorganic that a kind of content of microorganisms enriches, inorganic constituents after lignin adsorbs, its inhibitory action to soil microorganism can be substantially reduced, so the microbe survival environment such as the nitrogen-fixing bacteria added in the present invention to be got well, cultivate through simple amplification cultivation and adaptability simultaneously, substantially increase microorganism survival ability in soil, soybean root system is effectively helped to form root nodule, promote potassium, the absorption of phosphorus, improve the resistance against diseases of leguminous plant self.

Detailed description of the invention

Embodiment 1:

A kind of Semen sojae atricolor promotes the microorganism fertilizer of Nodule Growth, it is characterized in that, be made up of the raw material of following weight portion (Kg): black liquid 168,10wt% sulfuric acid solution 37,5% formalin 23, sodium sulfite 13, bone meal 13, brown sugar slag 17, ardealite 9, nitrogen-fixing bacteria 1, potassium solubilizing bacteria 0.7, phosphate solubilizing bacteria 0.3, edible fungi leftovers 29, Semen Phaseoli slag 13, Herba Astragali Melilotoidis (Herba Astragali Sinici) straw 19, calcium ammonium nitrate 11, ammonium molybdate 3, ferrous sulfate 9, zinc chloride 4, conch meal 7 and appropriate water.

A kind of Semen sojae atricolor promotes the preparation method of microorganism fertilizer of Nodule Growth, it is characterised in that include following step:

(1) black liquid is joined in reactor, it is heated to boiling, steaming and decocting is concentrated into original 1/3, then heating in water bath is turned to 50 ~ 60 DEG C, insulation, it is slowly stirred 30 ~ 40min with the rotating speed of 120 ~ 150r/min, it is simultaneously introduced 10wt% sulfuric acid solution, sucking filtration obtains lignin particles, crosses clear water and washs 3 ~ 5 times, finally standby after vacuum drying；

(2) lignin particles of (1) gained is joined in the container filling 5% formalin, regulation pH is 10 ~ 11, it is heated to 70 ~ 80 DEG C of insulation reaction 1.2 ~ 1.5h, pH value determination is addition sodium sulfite after neutrality, improve and at temperature 130 ~ 150 DEG C, react 3 ~ 4h, again wash and be dried, obtaining modified lignin particles；

(3) mixed edible bacterium leftover bits and pieces, Semen Phaseoli slag, Herba Astragali Melilotoidis (Herba Astragali Sinici) straw and other following residual components being not directed to, add the water of 2 ~ 3 times of its weight portions, water temperature 40 ~ 50 DEG C is soaked 2 ~ 3 days, then filter excessive moisture and pulverize slagging, spray the nitrogen-fixing bacteria of 10 times of use water dilution, potassium solubilizing bacteria, phosphate solubilizing bacteria mixture, heap fermentation 6 ~ 10 angel's Organic substance covers with mycelia thing；

(4) mixing ammonium nitrate calcium, ammonium molybdate, ferrous sulfate and zinc chloride, adds water in the ratio of material-water ratio 1:3 ~ 4, heated and stirred the lignin particles obtained by adding (2), and standing adsorption carries out ion exchange process, continues 20 ~ 30min；

(5) half organic fermentation materials of mixing (3) and the mixing and absorption inorganic nutrient solution described in (4), be watered holding water content 60 ~ 70%, slurry-spraying pelletizing in right amount.

A kind of Semen sojae atricolor using the present invention promotes the microorganism fertilizer planting experiment for crops Semen sojae atricolor of Nodule Growth, select 5 mu of soybean planting bases, need to use as main fertilizer according to soybean planting using the fertilizer of the present invention, 5 mu of Semen sojae atricolor of matched group use common fertilizer according to general planting method, result shows: Semen sojae atricolor and each side growing way of experimental group are superior to matched group, the fertilizer holding time in soil is longer, the root nodule growing way of soybean root system becomes apparent from, and is also better than the Semen sojae atricolor of matched group in terms of resistance.

Claims (2)

1. the microorganism fertilizer of a Semen sojae atricolor promotion Nodule Growth, it is characterized in that, be made up of the raw material of following weight portion: black liquid 150 ~ 180,10wt% sulfuric acid solution 30 ~ 40,5% formalin 20 ~ 30, sodium sulfite 10 ~ 15, bone meal 10 ~ 14, brown sugar slag 15 ~ 20, ardealite 7 ~ 10, nitrogen-fixing bacteria 1 ~ 2, potassium solubilizing bacteria 0.5 ~ 0.8, phosphate solubilizing bacteria 0.2 ~ 0.5, edible fungi leftovers 26 ~ 30, Semen Phaseoli slag 10 ~ 14, Herba Astragali Melilotoidis (Herba Astragali Sinici) straw 16 ~ 22, calcium ammonium nitrate 10 ~ 13, ammonium molybdate 2 ~ 4, ferrous sulfate 6 ~ 10, zinc chloride 4 ~ 7, conch meal 5 ~ 8 and appropriate water.

A kind of Semen sojae atricolor promotes the preparation method of microorganism fertilizer of Nodule Growth, it is characterised in that include following step:

(1) black liquid is joined in reactor, it is heated to boiling, steaming and decocting is concentrated into original 1/3, then heating in water bath is turned to 50 ~ 60 DEG C, insulation, it is slowly stirred 30 ~ 40min with the rotating speed of 120 ~ 150r/min, it is simultaneously introduced 10wt% sulfuric acid solution, sucking filtration obtains lignin particles, crosses clear water and washs 3 ~ 5 times, finally standby after vacuum drying；

(2) lignin particles of (1) gained is joined in the container filling 5% formalin, regulation pH is 10 ~ 11, it is heated to 70 ~ 80 DEG C of insulation reaction 1.2 ~ 1.5h, pH value determination is addition sodium sulfite after neutrality, improve and at temperature 130 ~ 150 DEG C, react 3 ~ 4h, again wash and be dried, obtaining modified lignin particles；

(3) mixed edible bacterium leftover bits and pieces, Semen Phaseoli slag, Herba Astragali Melilotoidis (Herba Astragali Sinici) straw and other following residual components being not directed to, add the water of 2 ~ 3 times of its weight portions, water temperature 40 ~ 50 DEG C is soaked 2 ~ 3 days, then filter excessive moisture and pulverize slagging, spray the nitrogen-fixing bacteria of 10 times of use water dilution, potassium solubilizing bacteria, phosphate solubilizing bacteria mixture, heap fermentation 6 ~ 10 angel's Organic substance covers with mycelia thing；

(4) mixing ammonium nitrate calcium, ammonium molybdate, ferrous sulfate and zinc chloride, adds water in the ratio of material-water ratio 1:3 ~ 4, heated and stirred the lignin particles obtained by adding (2), and standing adsorption carries out ion exchange process, continues 20 ~ 30min；

(5) half organic fermentation materials of mixing (3) and the mixing and absorption inorganic nutrient solution described in (4), be watered holding water content 60 ~ 70%, slurry-spraying pelletizing in right amount.