CN105716905A - Sample collecting and detecting method - Google Patents
Sample collecting and detecting method Download PDFInfo
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- CN105716905A CN105716905A CN201610101325.8A CN201610101325A CN105716905A CN 105716905 A CN105716905 A CN 105716905A CN 201610101325 A CN201610101325 A CN 201610101325A CN 105716905 A CN105716905 A CN 105716905A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 135
- 238000001514 detection method Methods 0.000 claims abstract description 45
- 238000012360 testing method Methods 0.000 claims description 273
- 238000007789 sealing Methods 0.000 claims description 18
- 238000002835 absorbance Methods 0.000 claims description 7
- 230000008595 infiltration Effects 0.000 claims description 4
- 238000001764 infiltration Methods 0.000 claims description 4
- 230000003111 delayed effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 description 13
- 239000007789 gas Substances 0.000 description 12
- 239000012982 microporous membrane Substances 0.000 description 12
- 239000004033 plastic Substances 0.000 description 7
- 229920003023 plastic Polymers 0.000 description 7
- 239000000853 adhesive Substances 0.000 description 6
- 230000001070 adhesive effect Effects 0.000 description 6
- 210000002700 urine Anatomy 0.000 description 6
- 239000007787 solid Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000012491 analyte Substances 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 239000010839 body fluid Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229950000845 politef Drugs 0.000 description 3
- 229920002635 polyurethane Polymers 0.000 description 3
- 239000004814 polyurethane Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- -1 eluate Substances 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000007779 soft material Substances 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 210000003756 cervix mucus Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Abstract
The invention provides a liquid sample collecting and detecting method. The method comprises the steps that a sample cavity and a detection cavity communicated with the sample cavity with a channel are provided; a sample enters the sample cavity and enters the detection cavity along the channel; the sample reaches a first position of the detection cavity, wherein a device which is connected to the detection cavity and used for controlling the liquid level of the detection cavity enables the sample to reach the first position, and the device used for controlling the liquid level in the detection cavity enables the sample to reach a second position of the detection cavity before reaching the first position, in other words, the sample enters the sample cavity and then enters the detection cavity along the channel, the sample reaches the second position of the detection cavity, and then the sample reaches the first position of the detection cavity. Thus, the device can quantitatively detect the sample, and meanwhile the sample can be detected in a delayed mode while quantitative detection is achieved.
Description
The application is Chinese invention patent application, application number: the divisional application of 201110009577.5.
Technical field
The present invention relates to the apparatus and method of a kind of sample collection and detection, especially the apparatus and method of detection by quantitative sample after sample collection.
Background technology
At medical diagnostic field, body fluid detection is relatively common detection.Utilize detecting device or detection cup to collect detection liquid sample, and judge whether liquid sample contains analyte, be a kind of commonplace method.Such detecting device or detection cup generally require that sample cup is collected in sample container, and person skilled is inserted a detectable bar and makes a part of submergence of reagent strip in the sample, take out reagent strip and read testing result after the some time.The detection of this mode, technical staff may touch sample, and crisis is healthy or makes sample contaminated.In order to avoid this risk, it is necessary to be operated plus after closure body on sample collection container.At present, have many obturators, such as US Patent No. 4,976,923, US5,429,804, and US6,726,879 disclosed devices.These devices are test strip to be fixed on the lid of detecting device.During use, container upset or inclination make sample infiltration detectable bar detect.U.S. Patent application US2003/0027359A1 disclosed in 6 days February in 2003 discloses a kind of detection urine cup.This detection urine cup still needs to promote pillar piston movement to make fluid sample flow out from cup cavity and moisten detecting element with push rod after lid lid to the opening of cup.Chinese patent application 200510113911.5 discloses a kind of detecting device for liquid sample, and in this device, detectable bar is positioned on bowl cover, has a pipe between bowl cover and cup, makes liquid entrance bowl cover detect by liquid sample is pressed into pipe.In actual application, not only sample is carried out qualitative analysis, in addition it is also necessary to carry out detection by quantitative.It addition, part sample is not required to detect immediately after collection, until after the regular hour, then carry out detection and the interpretation of result of sample.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of sample collection and detecting device, when using this device, it is possible to makes a number of sample enter test chamber, and detects with regard to this fixing sample size.Namely the detection limit of sample can be fixing, it is possible to detects quantitative sample.
On the one hand, the present invention provides a kind of device, and including sample chamber and test chamber, wherein test chamber is connected by passage with sample chamber；It addition, this device also includes controlling the device of liquid level in test chamber, this control device is connected in test chamber.In one embodiment, in this control test chamber, the device of liquid level makes sample have primary importance in test chamber.
In the present invention, after liquid sample enters sample chamber, the passage that this sample can connect with sample chamber along test chamber enters test chamber, when sample enter test chamber reach a certain amount of after, being connected in the controls test chamber in test chamber the device of liquid level makes test chamber become a sealing cavity.And when the gas in this annular seal space body and fluid pressure reach poised state, the liquid sample in sample chamber would not enter back into the test chamber of sealing, sample forms a liquid level in test chamber, i.e. sample primary importance in test chamber.After sample is fixed on primary importance, the sample size in test chamber will not change, and therefore, it can carry out detection by quantitative.In common detecting device, in sample chamber, the liquid level difference of sample can cause that the sample size entering in test chamber is different so that in test chamber the position of sample can because of sample add number and different.Such detecting device does not just reach the detection by quantitative of fixed amount, it is also difficult to be applied to non-professional operator.And apparatus of the present invention control the device of liquid level in test chamber and is arranged on the position of test chamber and may decide that test chamber becomes gas flow when sealing cavity in cavity, thus determining sample primary importance.Therefore, the sample in test chamber will not be how many and change with the sample in sample chamber, it is achieved real quantitative pattern detection；Meanwhile, different people operates this device also can reach identical result, is particularly well-suited to family or non-specialist operators uses.
In one preferred embodiment, in order to ensure to obtain the sample of stabilizing amount, the position that the device of this control liquid level is connected in test chamber is not less than the communicating passage of sample chamber and test chamber.When this device and passage are contour, sample is after entering test chamber, and sample entrance sample chamber is all sealed by sample along with the device of the increase of sample, passage and this control liquid level, and test chamber is sealed；And when this device is higher than communicating passage, after sample enters test chamber, first this interface channel can be sealed, now, test chamber only can be communicated with the external world by the device of control liquid level, when sample is further added by, controls the device of liquid level in test chamber and sealed, so that test chamber becomes one seals cavity.Two ways all makes test chamber be sealed, and when the pressure of liquid sample entered in the test chamber sealed and the gas pressure of sealing reach to balance, sample does not enter back into test chamber, forms fixing liquid level, completes detection by quantitative.
In another embodiment, in the control test chamber of the present invention, the device of liquid level can also make sample have the second position in test chamber.Namely in this control test chamber, the device of liquid level cover degree can make sample have primary importance and two fixing sample sizes of the second position in test chamber.In the present embodiment, sample enters sample chamber and enters test chamber by passage, after sample seals communicating passage, the device of this control liquid level makes test chamber become one and seals cavity, and liquid sample rests on the second position because the gas-liquid pressure in annular seal space body balances.When needs increase sample size, the device controlling liquid level makes test chamber communicate with ambient atmos, and the air pressure in test chamber reduces, and liquid sample travels further into test chamber because the air pressure in cavity reduces.After the device controlling liquid level is again sealed off test chamber, the gas-liquid pressure balance in this new annular seal space body makes the liquid level that sample is maintained at the second position.In this embodiment, the sample size not only entering test chamber can control, and can realize detection by quantitative.And also can control because sample enters time of test chamber, it is possible to achieve the collection of sample and detection split carry out, and namely the collection of sample and detection process can separate, it is possible to detect while collection, it is also possible to first collect sample, after detect；And can time delay detection sample.
In one preferred embodiment, in this control test chamber, the device of liquid level is between the primary importance and the second position of test chamber.
In the present invention, in this control test chamber, the device of liquid level has the function making the liquid of entrance test chamber be fixed on certain position, and this device tool there are ways to realize this function.In one embodiment, use the valve with opening and closing function to be arranged in test chamber, controlled the amount of liquid in test chamber by the opening and closing of valve.
In another embodiment, in this control test chamber, the device of liquid level can include the parts of permeable watertight, and these permeable watertight parts have and can pass the gas through, but can not make the function that liquid passes through.These parts are arranged in test chamber, it is ensured that test chamber communicates with ambient atmos and the liquid of intracavity can not flow out to the external world by these parts.After liquid sample adds apparatus of the present invention, being flowed into test chamber by sample chamber and passage, because of the existence of permeable watertight parts, test chamber communicates with ambient atmosphere, and air pressure is identical, namely also identical with the air pressure in sample chamber；Now, because of in sample chamber with the relation of liquid level difference in test chamber, liquid sample enters test chamber always.When liquid arrives these permeable watertight parts, because of its waterproof function, liquid will not flow or penetrate into outside test chamber.When liquid increases further, these permeable watertight parts by liquid seal, test chamber becomes sealing cavity.When reaching gas-liquid pressure balance in annular seal space body, liquid sample rests on the certain position in test chamber, i.e. primary importance.In some detailed description of the invention, these permeable watertight parts are that the material with this function is made, such as politef (PTFE) series of micropores film, polyurethane (TPU) series of micropores film etc.；In another specific embodiment, these permeable watertight parts are the aperture being arranged in test chamber, and this hole diameter is not more than the diameter of the film that liquid is formed because of surface tension.
In the embodiment that another one is more excellent, the device of this control liquid level can be breathed freely by one but fluid-tight parts and potted component combine, and potted component is used for sealing the parts of this permeable watertight.Permeable watertight parts and potted component can be bonded together, it is also possible to split and come.When both are bonded together and are connected in test chamber, it is possible to make sample have set, i.e. a second position in test chamber；After the two separates, it is possible to make sample have another position in test chamber, i.e. primary importance.After liquid sample adds sample chamber, enter test chamber through passage.Now, covered by potted component because of permeable watertight parts and seal, test chamber is only communicated with the external world by passage, when the sample of q.s enters test chamber, after sealing up the communicating passage of sample chamber and test chamber, test chamber becomes one and seals cavity, and intracavity liquid rests on fixing position after reaching gas-liquid pressure balance, i.e. the second position.After the potted component on permeable watertight parts is separated, the test chamber sealed is communicated with ambient atmos by permeable watertight parts, intracavity air pressure reduces, the further test chamber of liquid was not until having this parts, test chamber is sealed again, new gas-liquid pressure balance in annular seal space body makes sample be maintained at new liquid level, i.e. a primary importance.In some detailed description of the invention, these permeable watertight parts are that the material with this function is made, such as politef (PTFE) series of micropores film, polyurethane (TPU) series of micropores film etc.；And potted component can be made by the glue for having viscosity, as used the strong viscosity adhesive sticker that synthetic paper or plastic sheeting material (PET, PP, PE, PVC etc.) are surface layer.One more specifically in embodiment, adhesive sticker covers the device forming this control liquid level on microporous membrane.
The sample chamber of the present invention is used for collecting sample, especially liquid sample.Its profile is unrestricted, it is possible to for arbitrary shape, it is possible to meet the function accepting sample, particularly liquid sample, such as circle, oval or square etc..The material making sample chamber is also unrestricted, such as hard plastic, PVC；Or, it is possible to make for soft material, such as plastic sheeting etc..Test chamber is used for laying testing element, and completes the detection of sample.Its profile is also unrestricted, meets and can lay testing element and accept sample function.The material making test chamber can be identical with test chamber.As: PVC, plastics etc..
Test chamber is various with the connected mode of sample chamber, it is possible to be directly connected to, it is also possible to be indirectly connected with.As in one embodiment, test chamber and sample chamber are integrated, and are positioned at same cavity, are connected by perforate mode on the sidewall at the two interval.Separately in one embodiment, test chamber is connected in bottom with sample chamber, forms a passage.In another embodiment, sample chamber and test chamber are connected by valve and connect.
In apparatus of the present invention, the testing element being positioned at test chamber can be one or more.In one embodiment, testing element can be test strips.In a detailed description of the invention, in order to ensure the accuracy of test effect and test result, test strips position in test chamber is that its mark zone is higher than the sample primary importance in test chamber.In a more excellent embodiment, in order to realize the function of time delay detection, it is possible to make the sample absorbance district second position higher than test chamber of test strips.
In one embodiment, this sample collection detecting device also includes lid, and lid is used for sealing sample chamber.After sample is positioned at sample chamber, cover lid, sample chamber is sealed to ensure that sample is sealed in sample chamber.Lid is various with the closing mode of sample chamber, it is preferred that, it is possible to adopt the lid of press-button type.Specifically, installing a clasp on lid, install a clasp on the sidewall of sample chamber, clasp and clasp are worked in coordination, and can reach to close the purpose of sample chamber opening during cooperation.Additionally preferred, the closing mode of lid and sample chamber, also screw type.As, in a specific embodiment, the sidewall inwall of lid and cavity being respectively arranged with screw thread, work in coordination between screw thread and reach to close the purpose of sample chamber opening.In order to ensure lid and sample chamber sealing, it is possible to use potted component.In one embodiment, this potted component is " O " type ring, is positioned at cover bottom and sample chamber accent intersection.
In order to ensure that the liquid level that liquid sample enters in test chamber is fixed, in an embodiment, it is possible to install the parts of permeable watertight on the lid sealing sample chamber.Such as: use and there are politef (PTFE) the series of micropores film of this function, polyurethane (TPU) series of micropores film etc..In one specific embodiment, installing microporous membrane on lid, after lid covers sample chamber, the remainder of lid, the part particularly contacted with sample chamber seals sample chamber, to ensure that sample will not leak into outside.Now, microporous membrane makes sample chamber identical with ambient pressure, and the air pressure in sample chamber will not increasing or reducing and change because of liquid.When in control test chamber, the device of liquid level works, the liquid sample amount entering test chamber only controls along with the device controlling liquid level and changes, and will not change because of the air pressure in sample chamber.So that liquid sample primary importance in test chamber or the second position arbitrarily will not change because of the liquid level in the sample chamber that seals and air pressure, reach to control the purpose of fixing liquid level, also ensure that the fixing of sample size in test chamber.
On the other hand, the present invention also provides for a kind of method collecting and detect liquid sample, including providing sample chamber and the test chamber connected by passage with sample chamber；Sample is made to enter sample chamber and enter test chamber along passage；Sample is made to arrive the primary importance of test chamber.In one detailed description of the invention, the device of the control test chamber liquid level being connected in test chamber makes sample arrive primary importance.More excellent, in this control test chamber, the device of liquid level can make sample first arrive the second position of test chamber before arriving primary importance, even if sample enters sample chamber and enters test chamber along passage；Sample is made to arrive the second position of test chamber；Sample is made to arrive the primary importance of test chamber again.
In one embodiment, control the position of the device of liquid level in test chamber and be not less than described passage.
In another detailed description of the invention, control the device of liquid level in test chamber and include permeable watertight parts.More excellent, this device is made up of permeable watertight parts and the potted component for sealing these permeable watertight parts.
In one embodiment, when sample arrives primary importance, liquid sample infiltration testing element, testing element detection sample.
In another embodiment, after sample enters sample chamber, make lid combine and seal sample chamber.One preferred embodiment in, the gas permeable devices of lid makes sample chamber communicate with ambient atmosphere.As being provided with the parts of a permeable watertight on lid.Such as, microporous membrane etc..
Beneficial effect
The present invention controls to enter the sample size of test chamber by controlling the device of liquid level in test chamber, further, it is also possible to controls sample and enters the time of test chamber.Therefore, apparatus of the present invention can realize the detection by quantitative of sample, meanwhile, that meet detection by quantitative at the same time it can also be time delay detection sample.
Accompanying drawing explanation
Fig. 1 is the generalized section of apparatus of the present invention
Fig. 2 is that in apparatus of the present invention, sample adds sample chamber and arrives the generalized section of the second position
Fig. 3 is the generalized section of apparatus of the present invention cover lid
Fig. 4 controls the generalized section that liquid level apparatus structure separates in apparatus of the present invention
Fig. 5 is the generalized section that in apparatus of the present invention, sample arrives primary importance
Accompanying drawing mark explanation
This device 800；Sample chamber 100；Sample chamber accent 103；At the bottom of sample chamber chamber 102, sample chamber sidewall 101；Test chamber 200；Test chamber and sample chamber joint 201, another sidewall 202 of test chamber；Control the device 300 of liquid level；Permeable watertight parts 301；Seal member 302；Testing element 400；Testing element sample absorbance district 401；Testing element mark zone 403；Testing element results display area 404；Testing element pad 402；Sample chamber and test chamber passage 700；Sample is in the second position 601 of test chamber；Sample is in the primary importance 602 of test chamber；Lid 500, permeable watertight parts 501 on lid
Below the structure of liquid-sample collection device or these technical terms used are described further.
Sample
" sample " that the present invention refers to refers to any material needing chemical examination whether to there is and (or) analyze analyte concentration, or it needs to be determined that whether one or more samples exist and (or) the material of the analyte of quantity, or need to carry out the material of qualitative evaluation.Sample can be fluid sample, for instance liquid sample.Liquid sample includes body fluid, such as blood, serum, blood plasma, saliva, urine, tear, seminal fluid and bone marrow；Liquid sample can also be water sample, such as sea water, river, river etc., or from domestic water, municipal water use or industry water resource, runoff water or sewage；Sample can be food sample, such as milk and wine.Mucus, semisolid or solid sample can be used to make liquid, eluate, suspension or leachate equal samples.Such as, throat or genitals sample may be dipped in and make sample in liquid.Sample can include mixture or any relevant mixture, the cell suspending liquid in such as diluent or solution of liquid, solid and gas.Sample includes biological substance, such as cell, microorganism, organelle and biochemical complexes.Liquid sample solid, semisolid or the high-viscosity material such as non-liquid samples can be produced from such as soil, feces, tissue, organ, biological fluid or other nature.Such as, these solids or semi-solid samples can mix by the solution suitable with diluent one class.Sample can be macerated, freezing and defrosting, or other extracting method form liquid sample.Remaining particulate material can use the method that filtration or precipitation etc. are traditional to remove.In one example being embodied as, it is urine for the sample detected.
Testing element
" testing element " is any element performing test.In one embodiment, testing element is test strip.This test strip may be included on it has specific binding material pair for immunoassay.Test strip can be a kind of change by color upon completion of the assays or the test chemical bar of other signal intensity judged results.It is suitable for the sample that the present invention carries out detecting and includes but not limited to body fluid, the sample separated from biological tissue or body fluid.Such as, sample can be saliva, blood, serum, blood plasma, urine, Excreta, spinal fluid, vaginal secretion, mucus and tissue.Testing element is not limited to one, it is possible to be that two or more testing element is arranged in detecting device simultaneously, respectively different component in test sample.
Detailed description of the invention
In the following detailed description, accompanying drawing and corresponding explanatory note are only illustrate that the present invention can the mode of actable specific concrete scheme by way of example.We are not precluded from the present invention can also without prejudice to any other detailed description of the invention within the scope of the claims in the present invention.
In the device 800 of one embodiment of the present of invention, by sample chamber 100, test chamber 200 forms with controlling the device 300 of liquid level in test chamber, as shown in Figure 1 and Figure 5.Sample chamber 100 by sidewall 101, at the bottom of chamber 102 and accent 103 constitute.Test chamber is made up of sidewall, top 201 and bottom.In one specific embodiment, sample chamber 100 and test chamber 200 are positioned at same cavity, as shown in Figure 1, test chamber 200 shares a sidewall 101 and bottom 102 with sample chamber 100, test chamber top 201 is connected with sample chamber accent 103 and seals, and itself and sample chamber are separated by another sidewall 202 of test chamber.This device 300 controls the device 300 of liquid level in test chamber to be connected with test chamber 200, as it is shown in figure 1, may be mounted on the sidewall 101 of test chamber 200.In this control test chamber, the device 300 of liquid level can make the sample in entrance test chamber be maintained at primary importance 602；Meanwhile, this device can also make sample have two fixing liquid levels in test chamber: primary importance 602 and the second position 601.
The sample chamber 100 of the present invention is unrestricted with test chamber 200 profile, meets the function accepting sample and detection sample.In a specific embodiment, sample chamber 100 is cylindrical, and test chamber 200 is positioned at sample chamber, for arc-shaped structure.Sample chamber 100 and test chamber 200 material can be that PVC, plastic cement, plastics etc., sample chamber 100 and test chamber 200 can also deform or soft material composition for having, e.g., plastic sheeting, soft pvc etc..This sample chamber 100 and test chamber 200 can also be made for transparent material, " transparent " represents the material that a kind of light can pass through, thus under general indoor light, the bore hole of people is it can be seen that transparent material object below, and uses the people of this device 800 it can be seen that sample size under this transparency window or test result.
Sample chamber 100 and test chamber 200 are connected by passage and connect, and the mode of this passage 700 is not limit, but must meet the liquid sample in sample chamber and can pass through this channel flow function to test chamber.Therefore, passage 700 can be direct channel, it is also possible to for indirect path.Such as, when sample chamber and test chamber are integral type, it is possible to connect by connecting side-wall hole mode, it is also possible to a special channel connection is set between, then or, test chamber with sample chamber are installed valve and controls the connection of the two.When sample chamber and test chamber are split type, connected by a tubular conduit, it is also possible to valve is installed in tubular conduit and controls the connection of the two.In one detailed description of the invention, test chamber 200 and sample chamber 100 is connected on sidewall 202 perforate 700 by the two another and connects.This interface channel 700 may be located at the bottom of sample chamber 100, as shown in Figure 1.Preferably, this passage 700 is not higher than controlling the device 300 of liquid level in test chamber.
In one specific embodiment, in this control test chamber, the device 300 of liquid level is permeable watertight parts 301, these permeable watertight parts 301 are arranged on test chamber sidewall 101, and now, test chamber 200 is only communicated with ambient atmosphere by these parts 301 and passage 700.After liquid sample is added into this device 800, sample enters sample chamber 100.Sample chamber 100 and atmosphere, test chamber 200 is also by passage 700 and permeable watertight parts 301 and atmosphere, and namely test chamber 200 is identical with the gas pressure in sample chamber 100.So, sample passes through the passage 700 bottom sample chamber flow into test chamber 200 because sample chamber 100 liquid level is higher than test chamber 200 liquid level.Along with increasing of sample, passage 700 is sealed by sample gradually, and now, test chamber 200 can also be communicated with ambient atmos by permeable watertight parts 301, so test chamber 200 internal gas pressure is still identical with in sample chamber 100, sample goes successively to test chamber 200 because of the relation of liquid level.When the sample in test chamber 200 increases to and covers these permeable watertight parts 301, these parts 301 no longer communicate with ambient atmosphere, and test chamber 200 is sealed.Now, sample goes successively to the test chamber 200 of sealing, until when gas and liquid reach pressure balance in this cavity 200, sample is no longer flow into test chamber 200, is maintained at a fixed position in test chamber 200, i.e. the primary importance 602 of test chamber.
In another specific embodiment, in this control test chamber the device 300 of liquid level by one ventilative but fluid-tight parts 301 and seal member 302 combine.When permeable watertight parts 301 are bonded together with seal member 302, test chamber 200 is sealed by this device 300, does not communicate with the external world；After sample enters sample chamber 100, passage 700 can be passed through and arrive and be maintained at the second position 601 of test chamber 200, as shown in Figure 2.And after permeable watertight parts 301 and seal member 302 split and come, such as Fig. 4, by this device 300, test chamber 200 and ambient atmos can circulate, but liquid can not pass through this device 300 and extraneous circulation, now, test chamber 200 is communicated with ambient atmosphere by this device 300, and sample may proceed to enter test chamber 200, until liquid sample seals permeable watertight parts 301, last sample arrives and is maintained at the primary importance 602 of test chamber 200, as shown in Figure 5.
Being provided with testing element 400 in test chamber 200, testing element 400 can be one or more, detects multiple presence of analyte or content in a certain liquid sample simultaneously.In one embodiment, this testing element can be specially test strips 400, and test strips 400 has the sample absorbance district 401 adding sample and mark zone 403 and the region 404 of display test result.
In one embodiment, in order to ensure testing result, it is possible to make the mark zone 403 of testing element 400 be positioned on the primary importance 602 of test chamber.In one more specifically embodiment, having a pad 402 in the bottom of testing element, this pad can make the mark zone 403 of testing element 400 be positioned on primary importance 602.More excellent, this pad 402 makes the sample absorbance district 401 of test strips higher than the second position 601 simultaneously.This pad 402 can be mounted or located in test chamber 200；Can also being connected as a single entity with test chamber 200, e.g., pad 402 and test chamber 200, or pad 402 and test chamber 200, sample chamber 100 is single injection-molded.Pad 402 can adopt and not absorb liquid sample and do not occur the material of chemistry or biological respinse to make with liquid sample.
Assembly of the invention 800 can also include lid 500, and this lid 500 is used for sealing sample chamber 100, as shown in Figure 3.Lid 500 engages with the accent 103 of sample chamber 100 and forms sealing.In one specific embodiment, lid 500 is connected for spiral with sample chamber 100, and lid 500 medial wall has screw-in screw thread, and accent 103 lateral wall of sample chamber 100 has reception screw thread.In order to ensure that sample will not leak from sample chamber accent 103, it is possible to add potted component in lid 500, such as "O"-ring.When lid 500 covers sample chamber, this "O"-ring seals up the accent of sample chamber.
In order to ensure that the air pressure in sample chamber 100 will not change because of cover lid 500, it is possible to be provided with a ventilative device 501 on lid 500 so that sample chamber 100 always with atmosphere.Meanwhile, in order to ensure to enter this detecting device 800 sample will not seepage, this gas permeable devices 501 is simultaneously need to have fluid-tight function.In an embodiment, at the top of lid 500 equipped with ventilative but fluid-tight parts 501 so that sample chamber is after lid 500 seals sample chamber accent 103, and chamber air communicates with ambient atmosphere.
Below in conjunction with, shown in Fig. 1 to Fig. 5, the collection of this device one specific embodiment being described in detail and detects the idiographic flow of sample.As shown in Figure 1, test chamber 200 is positioned at sample chamber 100, test chamber 200 shares a sidewall 101 with sample chamber 100, another sidewall 202 separates test chamber 200 and sample chamber 100, test chamber top 201 is connected with sample chamber accent 103 and seals, and test chamber 200 is connected by opening 700 in the bottom of sidewall 202 with sample chamber 100.In the control test chamber being made up of microporous membrane 301 and adhesive sticker 302, the device 300 of liquid level is arranged on the sidewall 101 that test chamber is common with sample chamber.Testing element 400 is positioned at test chamber 200, and pad 402 is positioned at the bottom of testing element 400.When original state, adhesive sticker 302 covers to be formed on microporous membrane 301 and seals, and now, test chamber 200 is only communicated with the external world by passage 700.
By liquid sample, such as urine, from sample chamber accent 103 addition sample chamber 100, because sample chamber 100 is connected with test chamber 200, liquid sample can enter test chamber 200 along the passage 700 that the two is connected, as shown in Figure 2.When the liquid sample entered in test chamber 100 increases gradually, finally can seal up passage 700, now, the unique opening 700 communicated with the external world of test chamber 200 is sealed by sample, and test chamber 200 becomes one and seals cavity, in this sealing cavity 200, when the pressure of gas and liquid reaches to balance, the liquid sample in sample chamber 100 will not enter back into test chamber 200, thus forming a fixing liquid level, the i.e. sample second position 601 in test chamber, is shown in Fig. 2 shown in 601 positions.Now, being positioned at the bottom of the testing element 400 of test chamber 200 and sample absorbance district 401 second position 601 higher than test chamber, namely higher than sample liquid level, therefore, testing element 400 is not in contact with to sample, not being analyzed detection.
Will with permeable watertight parts 501, the lid 500 such as microporous membrane covers the accent 103 of the sample chamber 100 after adding sample, and sample chamber 100 forms liquid-tight envelope after being covered by lid 500.Because lid 500 there being ventilative microporous membrane 501 exist so that sample chamber 100 communicates with ambient atmosphere, so, sample chamber 100 will not change before and after lid 500 covers with the liquid level in test chamber 200.As it is shown on figure 3, the liquid level in test chamber remains at the second position 601.
Now, adhesive sticker 302 is torn from microporous membrane 301, as shown in Figure 4, after adhesive sticker 302 separates with microporous membrane 301, the microporous membrane 301 with permeable watertight performance makes test chamber 200 communicate with ambient atmosphere, now, gas pressure in test chamber 200 is reduced to atmospheric pressure, identical with the air pressure in sample chamber 100, and liquid level is higher than liquid level in test chamber 100 in sample chamber 100, forming pressure reduction, this pressure reduction makes the liquid sample in sample chamber 100 flow in test chamber 200 again by passage 700.When liquid sample increases to when sealing up microporous membrane 301 in test chamber 200, test chamber 200 becomes a cavity sealed again.Seal in cavity 200 at this, when liquid reach a certain amount of after, reach new vapor liquid equilibrium, now, liquid forms another liquid level fixed test chamber 200 in, i.e. sample primary importance 602 in test chamber, as shown in Figure 5.
When sample flows from the second position to primary importance, infiltration is positioned at the sample absorbance district 401 of the testing element of test chamber 200, and testing element 400 receives sample, carries out sample analysis and completes detection.
Claims (12)
1. the method collecting and detecting liquid sample, it is provided that sample chamber and the test chamber connected with sample chamber by passage；
Sample is made to enter sample chamber and enter test chamber along passage；
Sample is made to arrive the primary importance of test chamber；Wherein, the device of the control test chamber liquid level being connected in test chamber makes sample arrive primary importance；
In this control test chamber, the device of liquid level can make sample first arrive the second position of test chamber before arriving primary importance；That is: sample is made to enter sample chamber and enter test chamber along passage；Sample is made to arrive the second position of test chamber；Sample is made to arrive the primary importance of test chamber again.
2. the method for claim 1, it is characterised in that in described control test chamber, the position of the device of liquid level is not less than described passage.
3. method as claimed in claim 1 or 2, it is characterised in that in described control test chamber, the device of liquid level includes permeable watertight parts.
4. method as claimed in claim 3, it is characterised in that in described control test chamber, the device of liquid level includes permeable watertight parts and for sealing the potted component of these permeable watertight parts.
5. the method for claim 1, it is characterised in that when sample arrives primary importance, sample infiltration testing element, testing element detection sample.
6. method as claimed in claim 5, it is characterised in that after sample enters sample chamber, make lid combine and seal sample chamber.
7. method as claimed in claim 6, it is characterised in that the gas permeable devices of lid makes sample chamber communicate with ambient atmosphere.
8. a sample collection detecting device, including:
Sample chamber, test chamber, the two passes through channel connection；And the device of liquid level in control test chamber, this device is connected with test chamber: it is characterized in that, in this control test chamber, the device of liquid level makes sample have primary importance in test chamber；After in control test chamber, the permeable watertight parts of the device of liquid level separate with potted component, sample has primary importance in test chamber；In this control test chamber, the device of liquid level also makes sample have the second position in test chamber；The permeable watertight parts and the potted component that control the device of liquid level in test chamber seal after combining, and sample has the second position in test chamber.
9. device as claimed in claim 8, it is characterised in that control the device of liquid level in test chamber and include permeable watertight parts and for sealing the potted component of permeable watertight parts.
10. device as claimed in claim 9, it is characterised in that comprise the test strips of detection sample in test chamber.
11. device as claimed in claim 10, it is characterised in that test strips mark zone is higher than primary importance at detection intracavitary locations.
12. device as claimed in claim 11, it is characterised in that position in test chamber, the test strips sample absorbance district is higher than the second position.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610101325.8A CN105716905B (en) | 2011-01-15 | 2011-01-15 | A kind of sample collection detection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110009577.5A CN102589929B (en) | 2011-01-15 | 2011-01-15 | A kind of sample collection pick-up unit |
| CN201610101325.8A CN105716905B (en) | 2011-01-15 | 2011-01-15 | A kind of sample collection detection method |
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| CN201110009577.5A Division CN102589929B (en) | 2011-01-15 | 2011-01-15 | A kind of sample collection pick-up unit |
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| CN105716905A true CN105716905A (en) | 2016-06-29 |
| CN105716905B CN105716905B (en) | 2019-04-16 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107782906A (en) * | 2016-08-24 | 2018-03-09 | 杭州奥泰生物技术股份有限公司 | A kind of detection means and detection method |
| CN109342673A (en) * | 2018-12-14 | 2019-02-15 | 深圳市心月生物科技有限公司 | A kind of cream jug |
| CN111650389A (en) * | 2020-06-12 | 2020-09-11 | 杭州准芯生物技术有限公司 | Liquid detection cell and liquid analysis system |
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| US20040132091A1 (en) * | 2003-01-04 | 2004-07-08 | Ramsey James T. | Specimen collection and assay container |
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| US20060064032A1 (en) * | 2004-09-22 | 2006-03-23 | Medtox Scientific, Inc. | Systems and methods for collecting, testing and transporting liquid biological specimens |
| CN101556277A (en) * | 2009-01-05 | 2009-10-14 | 艾博生物医药(杭州)有限公司 | Detecting device |
| CN101876661A (en) * | 2009-05-08 | 2010-11-03 | 艾博生物医药(杭州)有限公司 | Device for analyzing analyte in liquid sample |
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| US20040132091A1 (en) * | 2003-01-04 | 2004-07-08 | Ramsey James T. | Specimen collection and assay container |
| US20060029517A1 (en) * | 2004-08-03 | 2006-02-09 | Hartselle R L | Specimen collection, storage, transportation and assaying device |
| US20060064032A1 (en) * | 2004-09-22 | 2006-03-23 | Medtox Scientific, Inc. | Systems and methods for collecting, testing and transporting liquid biological specimens |
| CN101556277A (en) * | 2009-01-05 | 2009-10-14 | 艾博生物医药(杭州)有限公司 | Detecting device |
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| CN107782906A (en) * | 2016-08-24 | 2018-03-09 | 杭州奥泰生物技术股份有限公司 | A kind of detection means and detection method |
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| CN111650389A (en) * | 2020-06-12 | 2020-09-11 | 杭州准芯生物技术有限公司 | Liquid detection cell and liquid analysis system |
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| CN105716905B (en) | 2019-04-16 |
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