CN105265362A - Cross breeding method for improving growth traits of slender mandarinfish - Google Patents
Cross breeding method for improving growth traits of slender mandarinfish Download PDFInfo
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 3
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- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 208000035240 Disease Resistance Diseases 0.000 abstract description 5
- 235000013372 meat Nutrition 0.000 abstract description 5
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a cross breeding method for improving growth traits of slender mandarinfish. The cross breeding method can be widely applied in large-scale breeding and belongs to the field of agricultural technology which is necessary for human life. The cross breeding method comprises the following main steps of parent fish cultivating, artificial inseminating, fish seed cultivating, cultivating in a pool, etc. Mandarin fish is selected as female parent fish and the slender mandarinfish is selected as male parent fish and furthermore intensive cultivation is performed. Artificial propagation technical measures of hasten parturition according to a male-and-female synchronous one-time exogenous hormone injection method, artificial dry-method inseminating, circular-channel water-flow hatching, etc., fish seeds are hatched. A segmented cultivation mode is adopted. High-palatability fish bait is applied. Furthermore breeding management in a seed period and a mature period are reinforced. The invention provides the method for improving the growth traits of the slender mandarinfish. The shape of the crossbred slender mandarinfish which is generated according to the method is different from that of the male parent fish and that of the female parent fish, and furthermore the growth advantage is better than that of the male parent fish. Furthermore the crossbred slender mandarinfish has advantages of high meat quality, delicious taste, high disease resistance, high growing speed, capability of eating dead fish, etc. The crossbred slender mandarinfish is a novel breed of the crossbred mandarin fish which facilitates artificial large-scale breeding.
Description
Technical field
The invention belongs to fish culture propagation technique field, particularly relate to and utilize fish interspecific cross breeding technique to produce the method with the long body mandarin fish of new varieties of obvious Morphological Features and growth vigor.Can be widely used in family, collective's mandarin fish cultivation, particularly large-scale cultivation is bred.
Background technology
Long body mandarin fish (Sinipercaroulei) and Mandarin fish (Sinipercachuatsi) belong to Perciformes ， Sushi section together, mandarin fish genus is high-grade freshwater fish kind.Long body mandarin fish is a kind in wild mandarin fish, and also known as long body mandarin fish, another name thick bamboo tube mandarin fish, color mandarin fish, color osmanthus are warm temperate zone mountain stream fish, the clear stream water environment of the many stone of happiness sediment.Long body mandarin fish is the special product fish of east China, is distributed widely in Zhejiang on the south the Changjiang river, Fujian, Jiangxi, Hunan, Guizhou and Deng Di waters, Guangxi.Compared with Mandarin fish, this meat quality of fish is solid, sting less between flesh, taste is more delicious, disease resistance good and the non-live fish that can ingest, and shortcoming is that growth is slow, individuality is less.Long body mandarin fish mainly wild resource in the market, the long body mandarin fish of commodity of few people's work cultivation.Mandarin fish life thermophilic 7 DEG C ~ 32 DEG C, optimum temperature 18 DEG C ~ 25 DEG C, taking live fish and shrimp as food throughout one's life from opening food, is predacious fish of dwelling at the bottom of the fresh water in Perciformes.Mandarin fish to the Qinling Mountains to the east of crosses the Yellow River to northeast Heilongjiang river through Central Yunnan to Sichuan from Vietnam of low latitudes all has distribution, extends to the Korea peninsula eastwards.From the early 1990s in last century, the cultivation of Mandarin fish has extensively been carried out in all parts of the country, achieves good economic and social benefit.
In fish culture propagation technique field, innovation and creation and Technique in Fishes insemination are hybridized and breeding method, and correlation technique also has a lot, and technical scheme beyond doubt success is feasible, but species variation obviously exists.According to the biological property of hybridization, if long body mandarin fish and Mandarin fish are hybridized, gene are reconfigured, through directive breeding, be expected to obtain the crossbreed possessing parent both sides' merit or produce parent unexistent new merit, the non-live fish that is namely expected to obtain to ingest, disease resistance be good, fast growth and the good hybridization mandarin fish cultivation new varieties of meat.But, there is not yet the technology that customs director's body mandarin fish and Mandarin fish hybridize cultivation aspect so far and report.
Summary of the invention
The object of the invention is, for developing high-grade fresh water culture fishery, to provide one to make full use of resource, more effectively improveing the hybridization cultural method of long body mandarin fish growth traits.The method produce hybridization mandarin fish has obvious growth vigor compared with male parent, disease resistance is good; Morphologically different from female parent, maintain that Mandarin fish meat exquisiteness is soft, the feature of delicious flavour simultaneously.
The present invention is for achieving the above object, and the technical scheme of employing is as follows:
A hybridization cultural method for long body mandarin fish growth traits, the method comprises the following steps:
(1) parent population is introduced and reinforced cultivating
Male parent parent population selects 1 age more than and wild long body mandarin fish more than body weight 0.5kg, maternal parent population selects 2 ages more than and healthy and strong original seed Mandarin fish more than body weight 1kg, they are placed in different net cage or pond respectively and carry out reinforced cultivating, the cycle of gonadal development of maternal Mandarin fish and the long body mandarin fish of male parent can synchronously be carried out;
(2) artificial propagation
1. Juvenile stage: select belly to expand, soft, ovary profile obviously, the Mandarin fish raun of the soft and whippy gonad maturity of hand belly as female parent, select that indent in the middle of anus be blush, light pressure belly has milky seminal fluid to flow out and enter long body mandarin fish milter that namely water fall apart as male parent;
2. artificial induced spawning: take synchronous shot exogenous hormone method to hasten parturition to the parent of gonad maturity, checks after 24 hours and to hasten parturition situation, when parent population oestrus reach a climax be about to lay eggs time, immediately parent population has been caught, has carried out ovum artificial collection, semen collection;
3. artificial insemination: maternal parent fish selection gonopore takes on a red color, belly expands, soft, when facing upward abdomen in the middle of recessed healthy and strong Mandarin fish, male parent parent fish selection gonopore is light color, the light belly adularescent seminal fluid that squeezes flows out, meets the namely loose healthy and strong spot mandarin fish of water, father, maternal parent population ratio are 1: 3
Adopt dry towel parcel parent population, first long for male parent body mandarin fish sperm is extruded, add 0.75% sodium chloride solution being equivalent to 5 times of sperm volumes to dilute, pour in fish-egg again and stir, add a small amount of clear water to stir a little, leave standstill 3 minutes, with clean water several, putting into circuit hatching, is 3:1 in order to adopt maternal quantity and the male parent quantitative proportion of ovum and semen collection;
4. hatch: hatching adopts circuit Lotic hatching, every cube of water body enters 3 ~ 40,000, fertilized egg, and guarantee to hatch water quality reaching standard, timely removing circuit or the egg membrane of hatching in cylinder, control the water velocity of circular hatching channal between the incubation period well, fry puberty flow rates demand fertilized egg can all be stirred but acutely not stir;
(3) cultivate seedling stage
Take segmentation cultivate mode, rupture of membranes is thrown something and fed after 2 days agreeable to the taste bait fry; Four large Chinese carp fries of after opening 3 days firm membranes of throwing something and feeding; Opening proceeds to cement from circuit after 4 days and cultivates; Put into pond when reaching 1cm and carry out the cultivation of special pond; And adjust in time according to forage fiss volume residual in fishpond at any time and supplement, and carry out water quality management and disease control work;
(4) pond culture
Above-mentioned hybridization mandarin fish seedling, forwards pond culture through nurture body long dozen to 3 cm, before hybridization mandarin fish puts into pond, cultivates the bait of bait fry as hybridization Siniperca kneri of 2 cm in the pool.
Further, parent population in described step (1) is introduced and hot housing, selection of time in the fall after, male parent parent population long body mandarin fish cultivates throw something and feed agreeable to the taste live fish or fish block, and maternal parent population Mandarin fish cultivates agreeable to the taste live fish of then can throwing something and feeding.
Further, Second Year adopts flowing water to irritate after the beginning of spring during mid-April, impels parent's gonadal maturation; Adhere to morning, patrol the pool evening, change of water quality and parent population active situation are observed in attention, carry out raising daily record; Find fish disease, treat in time; Again water is changed once every 15 ~ 20 days; Lay eggs the previous moon, should, every bath in 7 days, keep water quality pure and fresh.
Further, artificial induced spawning in described step (2), wherein oxytocic hormone kind has luteotropin releasing hormone d-ala analog (LRH-A), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM).
Further, maternal parent population injected dose is DOM4-7mg+LRH-A12 ~ 15 μ g+HCG1400 ~ 1700I/kg, and male parent parent population dosage reduces by half, and female milter is injected simultaneously, and the parent population after injection is placed in circuit to be supported temporarily, and stimulation by running water promotes that it is oestrused.
Further, the artificial insemination in described step (2), the good parent population of maturity can a shot, otherwise can divide biphasic injection, and during biphasic injection, two pins is spaced apart 12 hours;
Further, after injection exogenous hormone maternal parent population and male parent parent population be placed in same net cage to irritate to impel with the current of flow velocity 0.2m/s and oestrus, ovulate, maternal parent population and male parent parent population to be picked up for 24 ~ 28 hours after injection exogenous hormone starts to check, when the maternal parent population belly of light pressure has ovum to flow out, maternal parent population and male parent parent population are separated, carries out dry method of artificial fertilization.
Further, the hatching in described step (2), can strengthen flow velocity before and after fry membrane a little, controls at about 0.5m/s; Fry energy autonomous swimming flow velocity, controls as 0.2m/s.
Further, seedling stage in described step (3) cultivates, and rupture of membranes agreeable to the taste bait fry of throwing something and feeding after 2 days is megalobrama amblycephala, dace seedling etc., and feeding volume is 4 ~ 28 tail forage fisses/every tail hybridization mandarin fish seedling, and feeding volume is cumulative with age in days.
Further, pond culture in described step (4), supplement in time according to the quantity of forage fiss in the pool and the quantity of hybridization mandarin fish afterwards, carry out sterilization, oxygenation, management work simultaneously, after cultivating half a year, therefrom select the hybridization mandarin fish obtaining and there is improvement growth traits.
The present invention, relative to prior art, has following technique effect:
The inventive method produce hybridization mandarin fish has obvious growth vigor compared with male parent, disease resistance is good; Morphologically different from female parent, maintain that Mandarin fish meat exquisiteness is soft, the feature of delicious flavour simultaneously.Present device requires low, and operation is easy to realize, and can be used for general fish reproduction place; for developing high-grade fresh water aquaculture; cultivate mandarin fish cultivation improved Varieties and provide a kind of cost-effective method completely newly, can improve cultured output and quality, applicable large-scale cultivation breeds operation.
Embodiment
Below the embodiment of the hybridization cultural method of a kind of long body mandarin fish growth traits disclosed by the invention is elaborated, and be not used to limit the scope of the invention.
One, parent population is introduced and reinforced cultivating
October, male parent parent population selects 1 age more than and wild long body mandarin fish more than body weight 0.5kg, maternal parent population selects 2 ages more than and healthy and strong original seed Mandarin fish more than body weight 1kg, they are placed in different net cage or pond respectively and carry out reinforced cultivating, build plastic tunnel, improve temperature of cultivation, hot housing, the cycle of gonadal development of maternal Mandarin fish and the long body mandarin fish of male parent synchronously can be carried out, thus ensure effective overlap of two kinds of mandarin fish breeding periods, the hybridization between being conducive to kind.Selection of time in the fall after.Male parent parent population long body mandarin fish cultivates throw something and feed agreeable to the taste live fish or fish block, and maternal parent population Mandarin fish cultivates agreeable to the taste live fish of then can throwing something and feeding, and Second Year adopts flowing water to irritate after the beginning of spring during mid-April, impels parent's gonadal maturation.
Adhere to morning, patrol the pool evening, change of water quality and parent population active situation are observed in attention, carry out raising daily record; Find fish disease, treat in time; Again water is changed once every 15 ~ 20 days; Lay eggs the previous moon, should, every bath in 7 days, keep water quality pure and fresh.
Two, artificial propagation
1. Juvenile stage: at the beginning of 4 months, checks that the synchronous maturing rate of parent reaches more than 80%.Artificial propagation can be carried out during water temperature about 22 ~ 28 DEG C.Select belly to expand, soft, ovary profile obviously, the Mandarin fish raun of the soft and whippy gonad maturity of hand belly is as female parent.Indent in the middle of anus is selected to be that blush, gently pressure belly have milky seminal fluid to flow out and enter the namely loose long body mandarin fish milter of water as male parent.
2. artificial induced spawning: take synchronous shot exogenous hormone method to hasten parturition to the parent of gonad maturity, oxytocic hormone kind has luteotropin releasing hormone d-ala analog (LRH-A), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), maternal parent population injected dose is DOM4-7mg+LRH-A12 ~ 15 μ g+HCG1400 ~ 1700I/kg, male parent parent population dosage reduces by half, and female milter is injected simultaneously.Parent population after injection is placed in circuit to be supported temporarily, and stimulation by running water promotes that it is oestrused.Check after 24 hours and to hasten parturition situation, when parent population oestrus reach a climax be about to lay eggs time (be Effect time from artificial induced spawning to reaching the time of oestrusing needed for climax), immediately parent population has been caught, has carried out ovum artificial collection, semen collection.
3. artificial insemination: parent population breeding water body temperature is 21 DEG C ~ 28 DEG C before and after artificial insemination, day and night temperature 3 ~ 5 DEG C is the most suitable.(also plastic tunnel can be built when parent population breeding water body water temperature reaches 22 DEG C, improve temperature of cultivation), (exogenous hormone can optional maleic acid DOM (DOM) to start synchronously to inject exogenous hormone to maternal parent population and male parent parent population, human chorionic gonadtropin (HCG), one or more in luteotropin releasing hormone d-ala analog (LRH-A)), adopt maleic acid DOM (DOM), the hormotone of human chorionic gonadtropin (HCG) and luteotropin releasing hormone d-ala analog (LRH-A), raun dosage is DOM4-7mg+LRH-A12 ~ 15 μ g+HCG1400 ~ 1700I/kg (raun body weight), milter injection volume is the half of raun.The good parent population of general maturity can a shot, otherwise can divide biphasic injection, and during biphasic injection, two pins is spaced apart 12 hours.After injection exogenous hormone maternal parent population and male parent parent population be placed in same net cage to irritate to impel with the current of flow velocity 0.2m/s and oestrus, ovulate.Maternal parent population and male parent parent population to be picked up for 24 ~ 28 hours after injection exogenous hormone starts to check, when the maternal parent population belly of light pressure has ovum to flow out, maternal parent population and male parent parent population is separated, carries out dry method of artificial fertilization.
It is maternal that parent fish selection gonopore takes on a red color, belly expands, soft, when facing upward abdomen in the middle of recessed healthy and strong Mandarin fish, male parent parent fish selection gonopore is light color, the light belly adularescent seminal fluid that squeezes flows out, meets the namely loose healthy and strong spot mandarin fish of water, and father, maternal parent population ratio are 1: 3.
Adopt dry towel parcel parent population, first long for male parent body mandarin fish sperm is extruded, add 0.75% sodium chloride solution being equivalent to 5 times of sperm volumes and dilute, pour in fish-egg again and stir, add a small amount of clear water and stir a little, leave standstill 3 minutes, with clean water several, put into circuit hatching.Be 3:1 in order to adopt maternal quantity and the male parent quantitative proportion of ovum and semen collection.
4. hatch: hatching adopts circuit Lotic hatching, and every cube of water body enters 3 ~ 40,000, fertilized egg, and guarantee to hatch water quality reaching standard, remove the egg membrane in circuit or hatching cylinder in time.Control the water velocity of circular hatching channal between the incubation period well, fry puberty flow rates demand fertilized egg can all be stirred but acutely not stir; Flow velocity can be strengthened a little before and after fry membrane, control to be advisable at about 0.5m/s; Fry energy autonomous swimming flow velocity, the controlled 0.2m/s of being made as is advisable.
Three, cultivate seedling stage
Take segmentation cultivate mode, rupture of membranes is thrown something and fed after 2 days agreeable to the taste bait fry, and as megalobrama amblycephala, dace seedling etc., feeding volume is 4 ~ 28 tail forage fisses/every tail hybridization mandarin fish seedling, and feeding volume is cumulative with age in days.Opening proceeds to cement from circuit after 4 days and cultivates, cement pit area 18 square metres, and the depth of water 0.8 meter puts 3000 tails for every square metre.Put into pond when reaching 1cm and carry out the cultivation of special pond, pond area is 2 mu, and the depth of water 1.2 meters puts 4000 tails in a suitable place to breed for every mu, the forage fiss of mandarin fish gross weight 5% ~ 10% of throwing something and feeding, and adjusts in time according to forage fiss volume residual in fishpond at any time and supplement.Carry out the work such as water quality management and disease control.Hybrid seedlings survival rate 87%.
Above-mentioned hybridization mandarin fish seedling of hatching two days, carries out cultivating that (density domination is at 10,000 tails/m at the cement pit of circuit or miniflow water
3within, flow control is at about 0.1m/s), the dace of firm membrane of throwing something and feeding, megalobrama amblycephala opening, four large Chinese carp fries of after opening 3 days firm membranes of throwing something and feeding.Along with growing up of hybridization mandarin fish seedling, bait fry is corresponding increase also, and the body of general control bait fry is long is about 60% of hybridization fry ofSinopera chuatsi body length.Forward in washing basin cultivate when hybridization mandarin fish seedling body reaches 1 cm, carry out sterilization, oxygenation, bait throwing in manage.
Four, pond culture
Above-mentioned hybridization mandarin fish seedling, pond culture is forwarded to 3 cm through nurture body long dozen, before hybridization mandarin fish puts into pond, the bait of bait fry as hybridization Siniperca kneri of 2 cm is cultivated in the pool, supplement in time according to the quantity of forage fiss in the pool and the quantity of hybridization mandarin fish later, carry out sterilization, oxygenation, management work simultaneously, after cultivating half a year, therefrom select the hybridization mandarin fish obtaining and there is improvement growth traits.
The foregoing is only the preferred embodiments of the present invention, the numerical value mentioned in the description of above-mentioned specification and number range are not limited to the present invention, just for the invention provides preferred embodiment, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a hybridization cultural method for long body mandarin fish growth traits, the method comprises the following steps:
(1) parent population is introduced and reinforced cultivating
Male parent parent population selects 1 age more than and wild long body mandarin fish more than body weight 0.5kg, maternal parent population selects 2 ages more than and healthy and strong original seed Mandarin fish more than body weight 1kg, they are placed in different net cage or pond respectively and carry out reinforced cultivating, the cycle of gonadal development of maternal Mandarin fish and the long body mandarin fish of male parent can synchronously be carried out;
(2) artificial propagation
1. Juvenile stage: select belly to expand, soft, ovary profile obviously, the Mandarin fish raun of the soft and whippy gonad maturity of hand belly as female parent, select that indent in the middle of anus be blush, light pressure belly has milky seminal fluid to flow out and enter long body mandarin fish milter that namely water fall apart as male parent;
2. artificial induced spawning: take synchronous shot exogenous hormone method to hasten parturition to the parent of gonad maturity, checks after 24 hours and to hasten parturition situation, when parent population oestrus reach a climax be about to lay eggs time, immediately parent population has been caught, has carried out ovum artificial collection, semen collection;
3. artificial insemination: maternal parent fish selection gonopore takes on a red color, belly expands, soft, when facing upward abdomen in the middle of recessed healthy and strong Mandarin fish, male parent parent fish selection gonopore is light color, the light belly adularescent seminal fluid that squeezes flows out, meets the namely loose healthy and strong spot mandarin fish of water, father, maternal parent population ratio are 1: 3
Adopt dry towel parcel parent population, first long for male parent body mandarin fish sperm is extruded, add 0.75% sodium chloride solution being equivalent to 5 times of sperm volumes to dilute, pour in fish-egg again and stir, add a small amount of clear water to stir a little, leave standstill 3 minutes, with clean water several, putting into circuit hatching, is 3:1 in order to adopt maternal quantity and the male parent quantitative proportion of ovum and semen collection;
4. hatch: hatching adopts circuit Lotic hatching, every cube of water body enters 3 ~ 40,000, fertilized egg, and guarantee to hatch water quality reaching standard, timely removing circuit or the egg membrane of hatching in cylinder, control the water velocity of circular hatching channal between the incubation period well, fry puberty flow rates demand fertilized egg can all be stirred but acutely not stir;
(3) cultivate seedling stage
Take segmentation cultivate mode, rupture of membranes is thrown something and fed after 2 days agreeable to the taste bait fry; Four large Chinese carp fries of after opening 3 days firm membranes of throwing something and feeding; Opening proceeds to cement from circuit after 4 days and cultivates; Put into pond when reaching 1cm and carry out the cultivation of special pond; And adjust in time according to forage fiss volume residual in fishpond at any time and supplement, and carry out water quality management and disease control work;
(4) pond culture
Above-mentioned hybridization mandarin fish seedling, forwards pond culture through nurture body long dozen to 3 cm, before hybridization mandarin fish puts into pond, cultivates the bait of bait fry as hybridization Siniperca kneri of 2 cm in the pool.
2. method according to claim 1, it is characterized in that, parent population in described step (1) is introduced and hot housing, selection of time in the fall after, male parent parent population long body mandarin fish cultivates throw something and feed agreeable to the taste live fish or fish block, and maternal parent population Mandarin fish cultivates agreeable to the taste live fish of then can throwing something and feeding.
3. method according to claim 1 and 2, is characterized in that, Second Year adopts flowing water to irritate after the beginning of spring during mid-April, impels parent's gonadal maturation; Adhere to morning, patrol the pool evening, change of water quality and parent population active situation are observed in attention, carry out raising daily record; Find fish disease, treat in time; Again water is changed once every 15 ~ 20 days; Lay eggs the previous moon, should, every bath in 7 days, keep water quality pure and fresh.
4. method according to claim 1 and 2, it is characterized in that, artificial induced spawning in described step (2), wherein oxytocic hormone kind has luteotropin releasing hormone d-ala analog (LRH-A), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM).
5. method according to claim 4, is characterized in that, maternal parent population injected dose is DOM4-7mg+LRH-A12 ~ 15 μ g+HCG1400 ~ 1700I/kg, male parent parent population dosage reduces by half, female milter is injected simultaneously, and the parent population after injection is placed in circuit to be supported temporarily, and stimulation by running water promotes that it is oestrused.
6. method according to claim 1 and 2, is characterized in that, the artificial insemination in described step (2), and the good parent population of maturity can a shot, otherwise can divide biphasic injection, and during biphasic injection, two pins is spaced apart 12 hours.
7. method according to claim 6, it is characterized in that, after injection exogenous hormone maternal parent population and male parent parent population be placed in same net cage to irritate to impel with the current of flow velocity 0.2m/s and oestrus, ovulate, maternal parent population and male parent parent population to be picked up for 24 ~ 28 hours after injection exogenous hormone starts to check, when the maternal parent population belly of light pressure has ovum to flow out, maternal parent population and male parent parent population are separated, carries out dry method of artificial fertilization.
8. method according to claim 1 and 2, is characterized in that, the hatching in described step (2), can strengthen flow velocity before and after fry membrane a little, controls at about 0.5m/s; Fry energy autonomous swimming flow velocity, controls as 0.2m/s.
9. method according to claim 1 and 2, it is characterized in that, seedling stage in described step (3) cultivates, and rupture of membranes agreeable to the taste bait fry of throwing something and feeding after 2 days is megalobrama amblycephala, dace seedling etc., feeding volume is 4 ~ 28 tail forage fisses/every tail hybridization mandarin fish seedling, and feeding volume is cumulative with age in days.
10. method according to claim 1 and 2, it is characterized in that, pond culture in described step (4), supplement in time according to the quantity of forage fiss in the pool and the quantity of hybridization mandarin fish afterwards, carry out sterilization, oxygenation, management work simultaneously, after cultivating half a year, therefrom select the hybridization mandarin fish obtaining and there is improvement growth traits.
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