CN105176968A - Biological degrading agent for degrading cyanide and preparing method and application of biological degrading agent - Google Patents
Biological degrading agent for degrading cyanide and preparing method and application of biological degrading agent Download PDFInfo
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Abstract
The embodiment of the invention discloses a biological degrading agent for degrading cyanide and a preparing method and application of the biological degrading agent. The preparing method comprises the steps that waste biomass is used for preparing an activation carrier; an efficient microbial flora is used for culturing hyphae, and an active cyanide degrading bacterium solution is further prepared through the processes of culturing, centrifuging and dispersing; the activation carrier is steeped into the active cyanide degrading bacterium solution, and the biological degrading agent is obtained through the processes of culturing, filtering and washing. Compared with a chemical restoring method in the prior art, in the method, no acid-base agent or oxidizing agent is used, a biological degrading mode is adopted, and damage to the soil structure and the biocenosis can be avoided.
Description
Technical field
The present invention relates to cyanid field, particularly a kind of biodegradation agent for prussiate of degrading, its preparation method and application.
Background technology
Prussiate, such as potassium cyanide or sodium cyanide, with the complex performance of its brilliance, are widely used in metallurgical, plating, manufacture, coking field, and more and more prussiate enters soil and accumulates in soil simultaneously.Because prussiate and iron-binding capacity are too strong, can destroy in organism in enzyme system containing iron molecule (as P450 etc.), thus have very toxic to the various biologies comprising the mankind.So soil cyanide pollution receives increasing concern at present.Prussiate has two kinds of existing waies in soil: a kind of exist with stable complex state, and its toxicity is lower, and risk is less; Another kind of exist with unstable complex state or free state, is called easily release state prussiate, and easily entering in organism and to cause organism to damage, risk is larger.So in cyanide polluted soil repair process, control and the removal of easily release state prussiate should be given priority to.
At present, mainly chemical redemption is repaired for cyanide polluted soil, namely in cyanide polluted soil, add a large amount of oxygenant, the easy release state cyanide oxidation in soil is become harmless nitride.But oxygenant can cause the serious change of Soil structure, even spoiled soil structure and coenosis.
Summary of the invention
The embodiment of the invention discloses a kind of biodegradation agent for prussiate of degrading, its preparation method and application, adopting chemical mode to repair spoiled soil structure existing for cyanide polluted soil and biocenological problem for solving.Technical scheme is as follows:
For a preparation method for the biodegradation agent of prussiate of degrading, comprise the following steps:
Abandoned biomass is placed in after activator soaks 12-18h, under the protection of rare gas element, calcines 3-5h, obtain precursor carrier; First use detergent washing, rear washed with de-ionized water, drying after the precursor carrier obtained being cooled, after pulverizing, carry out sterilising treatment, obtain activated carrier; Wherein, calcining temperature is 350-450 DEG C; Nitrate and/or the aqueous phosphatic of described activator to be volumetric concentration be 5%-15%; Nitrate and/or the aqueous phosphatic of described washing composition to be volumetric concentration be 3%-30%;
Microbial strains is inoculated in liquid nutrient medium, adds the CN that prussiate makes in this substratum
-concentration reaches 1-2mg/L, and constant temperature culture grows mycelia to microbial strains, adds identical liquid nutrient medium afterwards every 2-3 days in the liquid nutrient medium being vaccinated with microbial strains, and adds prussiate with by CN
-concentration improve 1-2mg/L, the amount of wherein said identical liquid nutrient medium is 5% ~ 40% of initial liquid nutrient medium volume; As the CN in substratum
-after concentration reaches 10-15mg/L, continue to cultivate 3-5 days, be separated, collect cultured microbial strains, and collected microbial strains is distributed in new liquid nutrient medium, obtain active cyanide degradation bacterium liquid; Described microbial strains comprises pseudomonas and/or Penicillium notatum;
Activated carrier is soaked in described active cyanide degradation bacterium liquid, is placed in shaking table and cultivates 30-40h; Filter, with concentration be 0.05-0.1mol/L, pH value is the phosphoric acid buffer washing and filtering gained solid of 7-8, obtains biodegradation agent.
Wherein, described abandoned biomass comprises: at least one in corn cob, Pericarppium arachidis hypogaeae, bagasse, coconut palm chaff.
Wherein, the nitrate in described activator and/or phosphoric acid salt comprise: at least one in iron nitrate, tertiary iron phosphate, magnesium nitrate and nitrocalcite.
Wherein, the nitrate in described washing composition and/or phosphoric acid salt comprise: at least one in ammonium nitrate, ammonium phosphate, SODIUMNITRATE, sodium phosphate.
Wherein, described microbial strains is the flora B350 of Biosystem company.
Wherein, described liquid culture based formulas is: KH
2pO
40.5-1.0g/L, K
2hPO
40.5-1.0g/L, MgSO
47H
2o0.1-0.2g/L, CaCl
20.1-0.2g/L, NaCl0.1-0.2g/L, MnSO
4h
2o0.01-0.05g/L, FeCl
20.01-0.02g/L, NH
4cl1.0-1.5g/L.
Wherein, after the pulverizing, operation of sieving also is comprised before sterilising treatment.
Wherein, described prussiate comprises: potassium cyanide and/or sodium cyanide.
Wherein, the application method of described biodegradation agent, comprises step: being joined by biodegradation agent containing easily discharging in the cyanide polluted soil of state prussiate, adding water to water-retaining quantity among field of soil, digging and biodegradation agent is evenly distributed, and keeps 12-20 days.
The embodiment of the present invention is disclosed loose porous for the biodegradation agent of prussiate of degrading, and to the high adsorption capacity of prussiate, effectively can reduce in soil and easily discharges state concentration of cyanide and prussiate is enriched to degradation agents surface.Meanwhile, the fixing active cyanogen degrading microorganism in degradation agents surface can prussiate be carbon source, to be degraded adsorbed prussiate by biological process.
What the embodiment of the present invention provided utilizes the method for biodegradation agent degraded prussiate not use any soda acid agent and oxygenant, adopts biological degradation mode, can not damage Soil structure and coenosis.
Embodiment
Adopt technical scheme of the present invention to prepare biodegradation agent, first aspect will prepare activated carrier:
Abandoned biomass is placed in after activator soaks 12-18h, under the protection of rare gas element, calcines 3-5h, obtain precursor carrier; First use detergent washing, rear washed with de-ionized water, drying after the precursor carrier obtained being cooled, after pulverizing, carry out sterilising treatment, obtain activated carrier.
Wherein, said predicate " abandoned biomass " can be understood as agricultural waste material, timber waste etc., such as, can be one or more in corn cob, Pericarppium arachidis hypogaeae, bagasse, coconut palm chaff.After activator immersion treatment is carried out to abandoned biomass, the activated carrier of gained can be made to produce vesicular structure, improve the adsorptive power of activated carrier, be more conducive to adsorb can degrading activity prussiate bacterial classification and be fixed in biodegradation agent surface.Said activator can be nitrate and/or aqueous phosphatic, and wherein nitrate and/or phosphoric acid salt can comprise: at least one in iron nitrate, tertiary iron phosphate, magnesium nitrate and nitrocalcite.Preferred volume concentration is nitrate and/or the aqueous phosphatic of 5%-15%, and more preferably volumetric concentration is 8%-12%, and treatment effect is better.The ratio of abandoned biomass and activator can be determined according to practical situation by technician, does not limit at this, and the ratio of abandoned biomass quality and activator volume can be soaked in the activator of 80-150ml for the abandoned biomass of every 100 grams.
Abandoned biomass after activator immersion treatment needs to calcine 3-5h under the protection of rare gas element, and calcining temperature can be 350-450 DEG C.Calciner required during calcining, can adopt the calciner that this area is conventional, as long as can meet the requirements of calcining temperature under protection of inert gas state, the present invention does not limit at this, such as, can be tube furnace etc.In addition, said rare gas element can be the conventional nitrogen, argon gas etc. in this area.
Obtain precursor carrier after having calcined, first carry out cooling process, be generally cooled to room temperature, and then precursor carrier is washed; Said washing comprises first uses detergent washing, use deionized water wash again, can effectively remove impurity in carrier like this, be more conducive to the carrying out of subsequent preparation process, the number of times of washing can be determined according to practical situation by technician, usually, can detergent washing 2-4 time be used, then use deionized water wash 3-5 time.Said washing composition can be understood as nitrate and/or aqueous phosphatic that volumetric concentration is 3%-30% as washing composition, and its washing effect is better.With in the process of detergent washing, can first with detergent washing 1-2 time that volumetric concentration is 15%-30%, then be detergent washing 1-2 time of 3%-10% by volumetric concentration, the residual of washing composition can be reduced like this, reach the effect improving carrier quality.
Precursor carrier drying after washing, after pulverizing, sterilising treatment, obtains activated carrier.Wherein bake out temperature can be 105-110 DEG C.Can first sieve after pulverizing, to ensure the consistence of particle diameter, the order number of sieve can be determined by technician, such as, can select 120 orders.Finally to carry out sterilising treatment, be specifically as follows: use ozone sterilization 10min.It should be noted that, dry, pulverize, sieve, ozone sterilization is the conventional working method in this area, the present invention is not specifically limited at this.
Adopt technical scheme of the present invention to prepare biodegradation agent, second aspect will carry out cultivation, the domestication of bacterial classification:
Microbial strains is inoculated in liquid nutrient medium, adds the CN that prussiate makes in this substratum
-concentration reaches 1-2mg/L, and constant temperature culture grows mycelia to microbial strains, adds identical liquid nutrient medium afterwards every 2-3 days in the liquid nutrient medium being vaccinated with microbial strains, and adds prussiate with by CN
-concentration improve 1-2mg/L, as the CN in substratum
-after concentration reaches 10-15mg/L, continue to cultivate 3-5 days, be separated, collect cultured microbial strains, and collected microbial strains is distributed in new liquid nutrient medium, obtain active cyanide degradation bacterium liquid.
In the present invention, selected can comprise pseudomonas and/or Penicillium notatum for cultivation, acclimated microorganism bacterial classification, and concrete, microbial strains can select the flora B350 of Biosystem company, and its content of microorganisms is 30-50 hundred million/g.
When cultivation, acclimated microorganism bacterial classification, substratum used can be liquid nutrient medium, and be made up of water and the nutritive salt placed wherein, liquid culture based formulas can be: KH
2pO
40.5-1.0g/L, K
2hPO
40.5-1.0g/L, MgSO
47H
2o0.1-0.2g/L, CaCl
20.1-0.2g/L, NaCl0.1-0.2g/L, MnSO
4h
2o0.01-0.05g/L, FeCl
20.01-0.02g/L, NH
4cl1.0-1.5g/L.In culturing process, start most the substratum used by inoculating and the substratum added every 2-3 days and the last substratum for disperseing collected microbial strains below, adopt identical formula, more convenient like this operation, certainly, technician also can adopt different formulas according to practical situation, and this is all fine.
The prussiate added in culturing process can be potassium cyanide, sodium cyanide and other one or more to potassium cyanide, in the similar prussiate of sodium cyanide character.When actually operating, the method that culturing micro-organisms bacterial classification makes it to grow mycelia can be: the liquid nutrient medium being vaccinated with microbial strains is placed in 30-37 DEG C of shaking table, constant temperature culture 2-4 days.
After microbial strains grows silk, new substratum to be supplemented every 2-3 days, and supplement new prussiate to improve the concentration of prussiate in substratum gradually, in actual mechanical process, each supplement new prussiate and can make CN
-concentration improve 1-2mg/L, so namely can reach cultivations, tame object, can prevent due to CN again
-concentration improve and too fastly cause bacterial classification mortality, make to cultivate, tame unsuccessfully.
The add-on of said identical liquid nutrient medium, can be 5% ~ 40% of initial liquid nutrient medium volume, be preferably 10%-30%, be more preferably 20%-25%.In addition, in actual mechanical process, when cultivating with 1g microbial strains, collecting cultured microbial strains and can be scattered in the new liquid nutrient medium of 50-200ml.
The concrete mode of said strain separating can be centrifugal separation, such as: be put in whizzer by cultured bacterial classification, with the centrifugal 5-40min of the rotating speed of 3500-5500 rev/min, removes container middle and upper part and divides liquid, obtain the bacterial classification after cultivating.
After preparing activated carrier and active cyanide degradation bacterium liquid, activated carrier can be soaked in the obtained active cyanide degradation bacterium liquid of said process, and be placed in the shaking table that rotating speed is 120 revs/min, be cultivate 30-40h under the condition of 30-37 DEG C in temperature; Filter, with concentration be 0.05-0.1mol/L, pH value is gained solid after the phosphoric acid buffer washing and filtering of 7-8, obtains biodegradation agent; Wherein, the ratio of activated carrier and active cyanide degradation bacterium liquid can be determined according to practical situation by technician, do not limit at this, the ratio that activated carrier quality and active cyanide degradation bacteria liquid amass can be soaked in 8-12ml active cyanide degradation bacterium liquid for the activated carrier of every 1g.
It should be noted that, above-mentioned filtration, washing operation are the conventional working method in this area, and the present invention is not specifically limited at this.
When applying the obtained biodegradation agent of aforesaid method and carrying out cyanide polluted soil reparation, can comprise the following steps: biodegradation agent is joined containing easily discharging in the cyanide polluted soil of state prussiate, add water to water-retaining quantity among field of soil, dig and biodegradation agent is evenly distributed, keep 12-20 days.
Wherein, add water to field capacity, be conducive to bacterial classification in biodegradation agent and keep greater activity.Dig and biodegradation agent is evenly distributed, be conducive to biodegradation agent and fully contact with the easy release state prussiate in soil, remove the prussiate in soil to greatest extent, make degraded prussiate better effects if.
Be described technical scheme of the present invention below in conjunction with specific embodiment, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
In the examples below, said percentage ratio if no special instructions, all refers to percent by volume.
Embodiment 1
Get Pericarppium arachidis hypogaeae 200g in 500mL beaker, add the iron nitrate aqueous solution 200ml of 10%, soak 12h; after solution all blots; gained solid is placed in tube furnace, and logical nitrogen protection, calcines 3h under 450 DEG C of conditions; aqueous ammonium nitrate solution successively with 15%, 3% after cooling respectively washs 1 time; use deionized water wash again 3 times, after 105 DEG C of oven dry, pulverize; after crossing 120 mesh sieves, logical ozone sterilization 10min, obtains activated carrier.
Get high effective microbial community B3501g, be inoculated in 100ml liquid nutrient medium, liquid culture based formulas is: KH
2pO
40.5g/L, K
2hPO
40.5g/L, MgSO
47H
2o0.2g/L, CaCl
20.1g/L, NaCl0.2g/L, MnSO
4h
2o0.01g/L, FeCl
20.01g/L, NH
4cl1.0g/L; Add potassium cyanide and make CN
-concentration is 2.0mg/L, is placed in 37 DEG C of shaking tables and cultivates 3 days, after growing mycelia, every 3 days, supplements 10ml substratum in substratum, adds potassium cyanide by CN simultaneously
-concentration improves 2.0mg/L, by CN
-concentration is increased to 4.0mg/L; Repeat abovementioned steps until CN
-concentration is 14.0mg/L, cultivates and takes out substratum after 5 days, centrifugal 10min under 5000 revs/min of rotating speeds, and precipitation collects bacterial classification, and is scattered in 200mL not containing CN
-aseptic liquid nutrient medium in, obtain active cyanide degradation bacterium liquid.
20g activated carrier is joined in 200mL active cyanide degradation bacterium liquid, be placed in 37 DEG C of shaking tables, cultivate 36h with 120 revs/min of rotating speeds, filtering, is 0.05mol/L by concentration, and pH value is the phosphate buffered saline buffer washing solid 3 times of 8, obtain biodegradation agent, be placed in 4 DEG C of refrigerators and preserve.
20g biodegradation agent being joined 200g, easily to discharge state cyanide content be 112.4mg/L, and total cyanogen amount is in the cyanide polluted soil of 482.9mg/L, adds water to water-retaining quantity among field of soil, digs and biodegradation agent is evenly distributed.Keep 14 days, adopt every day in pedotheque monitoring soil and easily discharge state cyanide content.Experimental result shows, and add biodegradation agent easily release state cyanide content reduction by 63.4% in soil after 2 days, total cyanogen amount reduces cyanide content after 3.6%, 14 days and reduces by 92.5%, and total cyanogen amount reduces by 19.7%.
Embodiment 2
Get coconut palm chaff 100g in 500mL beaker, add the tertiary iron phosphate aqueous solution 150ml of 12%, soak 18h; after solution all blots; gained solid is placed in tube furnace, and logical nitrogen protection, calcines 4h under 400 DEG C of conditions; ammonium phosphate solution successively with 20%, 4% after cooling respectively washs 1 time; use deionized water wash again 3 times, after 110 DEG C of oven dry, pulverize; after crossing 120 mesh sieves, logical ozone sterilization 10min, obtains activated carrier.
Getting high effective microbial community B3501g is inoculated in 75ml liquid nutrient medium, and liquid culture based formulas is: KH
2pO
40.7g/L, K
2hPO
40.7g/L, MgSO
47H
2o0.1g/L, CaCl
20.2g/L, NaCl0.15g/L, MnSO
4h
2o0.03g/L, FeCl
20.02g/L, NH
4cl1.2g/L; Add potassium cyanide and make CN
-concentration is 1.0mg/L, is placed in 35 DEG C of shaking tables and cultivates 2 days, after growing mycelia, every 2 days, supplements 15ml substratum in substratum, adds potassium cyanide by CN simultaneously
-concentration improves 1.0mg/L, by CN
-concentration is increased to 2.0mg/L; Repeat abovementioned steps until CN
-concentration is 10.0mg/L, cultivates and takes out substratum after 3 days, centrifugal 15min under 4500 revs/min of rotating speeds, and precipitation collects thalline, and is scattered in 150mL not containing CN
-aseptic liquid nutrient medium in, obtain active cyanide degradation bacterium liquid.
15g activated carrier is joined in 150mL active cyanide degradation bacterium liquid, be placed in 35 DEG C of shaking tables, cultivate 30h with 120 revs/min of rotating speeds, filtering, is 0.05mol/L by concentration, and pH value is the phosphate buffered saline buffer washing solid 3 times of 7, obtain biodegradation agent, be placed in 4 DEG C of refrigerators and preserve.
15g biodegradation agent being joined 150g, easily to discharge state cyanide content be 87.2mg/L, total cyanogen amount is in the cyanide polluted soil of 292.4mg/L, add water to water-retaining quantity among field of soil, stir 12 days, adopt every day in pedotheque monitoring soil and easily discharge state cyanide content.Experimental result shows, and add biodegradation agent easily release state cyanide content reduction by 51.6% in soil after 1 day, total cyanogen amount reduces cyanide content after 5.1%, 12 days and reduces by 90.3%, and total cyanogen amount reduces by 21.4%.
Embodiment 3
Get corn cob 500g in 1000mL beaker, add the magnesium nitrate aqueous solution 500ml of 8%, soak 15h; after solution all blots; gained solid is placed in tube furnace, and logical nitrogen protection, calcines 4.5h under 380 DEG C of conditions; sodium nitrate aqueous solution successively with 17%, 5% after cooling respectively washs 1 time; use deionized water wash again 5 times, after 105 DEG C of oven dry, pulverize; after crossing 120 mesh sieves, logical ozone sterilization 10min, obtains activated carrier.
Getting high effective microbial community B35010g is inoculated in 1000ml liquid nutrient medium, and liquid culture based formulas is: KH
2pO
40.65g/L, K
2hPO
40.65g/L, MgSO
47H
2o0.2g/L, CaCl
20.15g/L, NaCl0.15g/L, MnSO
4h
2o0.01g/L, FeCl
20.02g/L, NH
4cl1.3g/L; Add potassium cyanide and make CN
-concentration is 2.0mg/L, is placed in 37 DEG C of shaking tables and cultivates 4 days, after growing mycelia, every 3 days, supplements 300ml substratum in substratum, adds potassium cyanide by CN simultaneously
-concentration improves 2.0mg/L, by CN
-concentration is increased to 4.0mg/L; Repeat abovementioned steps until CN
-concentration is 15.0mg/L, cultivates and takes out substratum after 5 days, centrifugal 5min under 5500 revs/min of rotating speeds, and precipitation collects bacterial classification, and is scattered in 500mL not containing CN
-aseptic liquid nutrient medium in, obtain active cyanide degradation bacterium liquid.
25g activated carrier is joined in 250mL active cyanide degradation bacterium liquid, be placed in 37 DEG C of shaking tables and cultivate 40h with 120 revs/min of rotating speeds, filter, be 0.1mol/L by concentration, pH value is the phosphate buffered saline buffer washing solid 4 times of 7, obtains biodegradation agent, is placed in 4 DEG C of refrigerators and preserves.
25g biodegradation agent being joined 300g, easily to discharge state cyanide content be 153.8mg/L, total cyanogen amount is in the cyanide polluted soil of 531.1mg/L, add water to water-retaining quantity among field of soil, stir 20 days, adopt every day in pedotheque monitoring soil and easily discharge state cyanide content.Experimental result shows, and add biodegradation agent easily release state cyanide content reduction by 64.5% in soil after 4 days, total cyanogen amount reduces cyanide content after 5.4%, 20 days and reduces by 84.7%, and total cyanogen amount reduces by 15.3%.
Embodiment 4
Get bagasse 300g in 800mL beaker, add the calcium nitrate aqueous solution 240ml of 5%, soak 13h; after solution all blots; gained solid is placed in tube furnace, and logical nitrogen protection, calcines 5h under 350 DEG C of conditions; sodium phosphate aqueous solution successively with 30%, 10% after cooling respectively washs 2 times; use deionized water wash again 4 times, after 110 DEG C of oven dry, pulverize; after crossing 120 mesh sieves, logical ozone sterilization 10min, obtains activated carrier.
Getting high effective microbial community B3502g is inoculated in 200ml liquid nutrient medium, and liquid culture based formulas is: KH
2pO
40.75g/L, K
2hPO
40.75g/L, MgSO
47H
2o0.15g/L, CaCl
20.2g/L, NaCl0.15g/L, MnSO
4h
2o0.02g/L, FeCl
20.02g/L, NH
4cl1.2g/L; Add sodium cyanide and make CN
-concentration is 1.5mg/L, is placed in 30 DEG C of shaking tables and cultivates 4 days, after growing mycelia, every 2 days, supplements 10ml substratum in substratum, adds sodium cyanide by CN simultaneously
-concentration improves 1.5mg/L, by CN
-concentration is increased to 3.0mg/L; Repeat abovementioned steps until CN
-concentration is 12.0mg/L, cultivates and takes out substratum after 4 days, centrifugal 40min under 3500 revs/min of rotating speeds, and precipitation collects bacterial classification, and is scattered in 400mL not containing CN
-aseptic liquid nutrient medium in, obtain active cyanide degradation bacterium liquid.
10g activated carrier is joined in 80mL active cyanide degradation bacterium liquid, be placed in 30 DEG C of shaking tables and cultivate 35h with 120 revs/min of rotating speeds, filter, be 0.1mol/L by concentration, pH value is the phosphate buffered saline buffer washing solid 4 times of 8, obtains biodegradation agent, is placed in 4 DEG C of refrigerators and preserves.
10g biodegradation agent being joined 100g, easily to discharge state cyanide content be 105.6mg/L, total cyanogen amount is in the cyanide polluted soil of 329.5mg/L, add water to water-retaining quantity among field of soil, stir 15 days, adopt every day in pedotheque monitoring soil and easily discharge state cyanide content.Experimental result shows, and add biodegradation agent easily release state cyanide content reduction by 55.4% in soil after 2 days, total cyanogen amount reduces cyanide content after 4.2%, 15 days and reduces by 88.6%, and total cyanogen amount reduces by 17.9%.
Embodiment 5
Get corn cob and Pericarppium arachidis hypogaeae 400g in 800mL beaker, add the iron nitrate aqueous solution 600ml of 15%, soak 16h; after solution all blots; gained solid is placed in tube furnace, and logical nitrogen protection, calcines 3.5h under 420 DEG C of conditions; aqueous ammonium nitrate solution successively with 25%, 7% after cooling respectively washs 2 times; use deionized water wash again 5 times, after 105 DEG C of oven dry, pulverize; after crossing 120 mesh sieves, logical ozone sterilization 10min, obtains activated carrier.
Getting high effective microbial community B3505g is inoculated in 300ml liquid nutrient medium, and liquid culture based formulas is: KH
2pO
40.85g/L, K
2hPO
40.65g/L, MgSO
47H
2o0.1g/L, CaCl
20.15g/L, NaCl0.2g/L, MnSO
4h
2o0.04g/L, FeCl
20.01g/L, NH
4cl1.3g/L; Add potassium cyanide and make CN
-concentration is 1mg/L, is placed in 32 DEG C of shaking tables and cultivates 5 days, after growing mycelia, every 3 days, supplements 120ml substratum in substratum, adds potassium cyanide by CN simultaneously
-concentration improves 1.0mg/L, by CN
-concentration is increased to 2.0mg/L; Repeat abovementioned steps until CN
-concentration is 10.0mg/L, cultivates and takes out substratum after 3 days, centrifugal 25min under 4000 revs/min of rotating speeds, and precipitation collects bacterial classification, and is scattered in 300mL not containing CN
-aseptic liquid nutrient medium in, obtain active cyanide degradation bacterium liquid.
30g activated carrier is joined in 360mL active cyanide degradation bacterium liquid, be placed in 32 DEG C of shaking tables and cultivate 32h with 120 revs/min of rotating speeds, filter, be 0.08mol/L by concentration, pH value is the phosphate buffered saline buffer washing solid 3 times of 7, obtains biodegradation agent, is placed in 4 DEG C of refrigerators and preserves.
30g biodegradation agent being joined 300g, easily to discharge state cyanide content be 120.3mg/L, total cyanogen amount is in the cyanide polluted soil of 431.7mg/L, add water to water-retaining quantity among field of soil, stir 18 days, adopt every day in pedotheque monitoring soil and easily discharge state cyanide content.Experimental result shows, and add biodegradation agent easily release state cyanide content reduction by 61.2% in soil after 3 days, total cyanogen amount reduces cyanide content after 5.2%, 18 days and reduces by 91.1%, and total cyanogen amount reduces by 20.6%.
As can be seen from the various embodiments described above, adopt technical scheme of the present invention, in the cyanide polluted soil after process, easily release state cyanide content reduces by more than 80%, and total cyanogen amount reduces by more than 15%.Visible, technical scheme of the present invention easily discharges state prussiate high adsorption capacity in soil, and degradation treatment is effective.And the method for the degraded prussiate that the embodiment of the present invention provides does not use any soda acid agent and oxygenant, adopt biological degradation mode, can not damage Soil structure and coenosis.
Above a kind of biodegradation agent for prussiate of degrading provided by the present invention, its preparation method and application are described in detail.Apply specific embodiment herein to set forth principle of the present invention and embodiment, the explanation of above embodiment just understands method of the present invention and clou thereof for helping.It should be pointed out that for the person of ordinary skill of the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify the protection also falling into the claims in the present invention.
Claims (10)
1. for a preparation method for the biodegradation agent of prussiate of degrading, it is characterized in that, comprising:
Abandoned biomass is placed in after activator soaks 12-18h, under the protection of rare gas element, calcines 3-5h, obtain precursor carrier; First use detergent washing, rear washed with de-ionized water, drying after the precursor carrier obtained being cooled, after pulverizing, carry out sterilising treatment, obtain activated carrier; Wherein, calcining temperature is 350-450 DEG C; Nitrate and/or the aqueous phosphatic of described activator to be volumetric concentration be 5%-15%; Nitrate and/or the aqueous phosphatic of described washing composition to be volumetric concentration be 3%-30%;
Microbial strains is inoculated in liquid nutrient medium, adds the CN that prussiate makes in this substratum
-concentration reaches 1-2mg/L, and constant temperature culture grows mycelia to microbial strains, adds identical liquid nutrient medium afterwards every 2-3 days in the liquid nutrient medium being vaccinated with microbial strains, and adds prussiate with by CN
-concentration improve 1-2mg/L, the amount of wherein said identical liquid nutrient medium is 5% ~ 40% of initial liquid nutrient medium volume; As the CN in substratum
-after concentration reaches 10-15mg/L, continue to cultivate 3-5 days, be separated, collect cultured microbial strains, and collected microbial strains is distributed in new liquid nutrient medium, obtain active cyanide degradation bacterium liquid; Described microbial strains comprises pseudomonas and/or Penicillium notatum;
Activated carrier is soaked in described active cyanide degradation bacterium liquid, is placed in shaking table and cultivates 30-40h; Filter, with concentration be 0.05-0.1mol/L, pH value is the phosphoric acid buffer washing and filtering gained solid of 7-8, obtains biodegradation agent.
2. the preparation method of biodegradation agent as claimed in claim 1, it is characterized in that, described abandoned biomass comprises: at least one in corn cob, Pericarppium arachidis hypogaeae, bagasse, coconut palm chaff.
3. the preparation method of biodegradation agent as claimed in claim 1, it is characterized in that, the nitrate in described activator and/or phosphoric acid salt comprise: at least one in iron nitrate, tertiary iron phosphate, magnesium nitrate and nitrocalcite.
4. the preparation method of biodegradation agent as claimed in claim 1, it is characterized in that, the nitrate in described washing composition and/or phosphoric acid salt comprise: at least one in ammonium nitrate, ammonium phosphate, SODIUMNITRATE, sodium phosphate.
5. the preparation method of biodegradation agent as claimed in claim 1, it is characterized in that, described microbial strains is the flora B350 of Biosystem company.
6. the preparation method of biodegradation agent as claimed in claim 1, it is characterized in that, described liquid culture based formulas is: KH
2pO
40.5-1.0g/L, K
2hPO
40.5-1.0g/L, MgSO
47H
2o0.1-0.2g/L, CaCl
20.1-0.2g/L, NaCl0.1-0.2g/L, MnSO
4h
2o0.01-0.05g/L, FeCl
20.01-0.02g/L, NH
4cl1.0-1.5g/L.
7. the preparation method of biodegradation agent as claimed in claim 1, is characterized in that, after the pulverizing, also comprise operation of sieving before sterilising treatment.
8. the preparation method of biodegradation agent as claimed in claim 1, it is characterized in that, described prussiate comprises: potassium cyanide and/or sodium cyanide.
9. the biodegradation agent prepared of the method according to any one of claim 1-8.
10. the application method of biodegradation agent as claimed in claim 9, it is characterized in that, comprising: biodegradation agent being joined containing easily discharging in the cyanide polluted soil of state prussiate, adding water to water-retaining quantity among field of soil, dig and biodegradation agent is evenly distributed, keep 12-20 days.
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| CN110340138A (en) * | 2019-07-10 | 2019-10-18 | 内蒙古科技大学 | The method of biomass carbon enhancement microbiological reparation Cr (VI)-cyanide combined contamination soil |
| CN110803845A (en) * | 2019-11-27 | 2020-02-18 | 长沙凯天工研院环保服务有限公司 | Biological conditioner and method for improving dehydration performance of anaerobic digestion substances of kitchen waste |
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| CN111635648A (en) * | 2020-06-10 | 2020-09-08 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Degradation promoter prepared by layer-by-layer coating method and preparation and application thereof |
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