CN105021670A - Apparatus and method for analyzing concentration of cycloheximide based on surface acoustic wave series resonator biological tongue - Google Patents

Apparatus and method for analyzing concentration of cycloheximide based on surface acoustic wave series resonator biological tongue Download PDF

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CN105021670A
CN105021670A CN201510067409.XA CN201510067409A CN105021670A CN 105021670 A CN105021670 A CN 105021670A CN 201510067409 A CN201510067409 A CN 201510067409A CN 105021670 A CN105021670 A CN 105021670A
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drip chamber
cycloheximide
acoustic wave
surface acoustic
solution
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CN105021670B (en
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惠国华
郑钢英
汤旭翔
金姣姣
葛阳杨
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Zhejiang Gongshang University
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Zhejiang Gongshang University
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Abstract

The invention relates to an apparatus and method for analyzing the concentration of cycloheximide based on a surface acoustic wave series resonator biological tongue. The apparatus and method provided by the invention overcome the problem of high cost of usage of a concentration analyzer in the prior art. The apparatus comprises an acquisition workbench, an acquisition unit, a conversion unit and a treating unit, wherein the acquisition workbench comprises a seat body, the seat body is provided with a disc, the disc is provided with a plurality of liquid storage chambers, the lower part of one side of the disc is provided with a dropping liquid chamber, the bottom of the dropping liquid chamber is provided with a dropping liquid head, and the seat body is provided with a standing groove at a position below the dropping liquid head; the acquisition unit comprises a cell plate electrode and a surface acoustic wave resonator, the cell plate electrode is located in the standing groove and the surface acoustic wave resonator is connected with the conversion unit; and the conversion unit is connected with the treating unit. The apparatus and method have the advantages of simple structure, low making cost, capacity of automatic detection of the concentrations of a plurality of solutions, convenient and fast operation, reduction in workload of operation personnel and time, convenient and fast detection process and high precision.

Description

Based on the cycloheximide concentration analysis device and method of the biological tongue of surface acoustic wave series resonator
Technical field
The present invention relates to a kind of solution concentration detection technology, especially relate to a kind of cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator, and based on the cycloheximide concentration analysis of the biological tongue of surface acoustic wave series resonator.
Background technology
Often needing in the lab to detect the concentration of solution, when detecting solution concentration, usually use special concentration analyzer to detect, but concentration analyzer is expensive, also needs unit up to ten thousand at least, this considerably increases experimental cost.Therefore, be necessary to design a kind of structure simple, swift to operate, concentration detection apparatus with low cost, and the detection method of this pick-up unit.
Summary of the invention
The present invention mainly solves in prior art the problem adopting concentration analyzer cost high, provides a kind of structure simple, swift to operate, the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator that cost is low.
Present invention also offers a kind of cycloheximide concentration analysis based on the biological tongue of surface acoustic wave series resonator swift to operate.
Above-mentioned technical matters of the present invention is mainly solved by following technical proposals: a kind of cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator, comprise collecting work platform, collecting unit, converting unit and processing unit, described collecting work platform comprises pedestal, on pedestal, rotary setting has disk, disk is provided with multiple liquid storage cylinder around the center of circle, disk one side lower part is provided with drip chamber, described drip chamber top contacts with disk bottom, liquid outlet is provided with bottom fluid reservoir, liquid outlet is provided with the first solenoid valve, at drip chamber top, corresponding liquid outlet position is provided with inlet, liquid droping head is provided with on bottom described drip chamber, wiper mechanism and drainage mechanism is provided with in drip chamber, the below pedestal of liquid droping head is provided with standing groove, described collecting unit comprises the cell battery lead plate and SAW (Surface Acoustic Wave) resonator that are connected, cell battery lead plate is located in standing groove, described SAW (Surface Acoustic Wave) resonator is connected with converting unit, converting unit is connected with processing unit.Variable concentrations solution drips on cell battery lead plate and detects by collecting work platform of the present invention automatically respectively, SAW (Surface Acoustic Wave) resonator gathers reaction information on cell battery lead plate, output to converting unit with the form of frequency to turn, converting unit gathers the frequency signal of SAW (Surface Acoustic Wave) resonator, and convert digital signal to and send to processing unit, processing unit carries out resonance process to digital signal and obtains output signal-to-noise ratio value.After variable concentrations solution is detected, obtain the output signal-to-noise ratio value corresponding respectively with N number of digital signal, then obtain solution concentration forecast model according to fitting a straight line.Disk can place multiple strength solution, and rotate at set intervals according to setting and a liquid storage luminal solution is joined in drip chamber.Solution drips on cell battery lead plate by drip chamber, can discharge surplus solution and can clean drip chamber after a kind of strength solution detects.The present invention can analyze the concentration of variable concentrations solution automatically, simple to operation, waits for side always and manually changes and detect solution, alleviate operating personnel's workload, saved the time without the need to operating personnel.Drip chamber of the present invention has cleaning function, after a kind of solution of concentration is discharged, wiper mechanism can pass into water and clean, and pass into gas and dry up, can not mix with remaining liq before when ensure that another kind of strength solution enters drip chamber, make can not changing of solution concentration, ensure that the accuracy of ultimate analysis data.
As a kind of preferred version, described wiper mechanism comprises the water inlet and air intake opening that are communicated with drip chamber inside, water inlet is connected with water tank, air intake opening is connected with air pump, water inlet and air intake opening are respectively arranged with entering water electromagnetic valve and air inlet electromagnetic valve, described drainage mechanism comprises the freeing port be arranged on bottom drip chamber, and freeing port is provided with drain solenoid valve.Wiper mechanism cleans drip chamber.Freeing port is opened during cleaning, pass into clear water by water inlet and carry out water cleaning, pass into air and carry out drying in drip chamber after water cleaning by air intake opening, this solution added after making can not mix with Liquid Residue before, solution concentration can not be changed, ensure the accuracy of analysis result.
As a kind of preferred version, described liquid droping head is connected with drip chamber by micro pump.By micro pump, the solution in drip chamber was dropped on cell battery lead plate according to set amount and time.
As a kind of preferred version, oxygenation rabbling mechanism is provided with in drip chamber, oxygenation rabbling mechanism comprises rotating shaft, pipeline is provided with in rotating shaft, described rotating shaft is connected with air intake opening with water inlet, rotating shaft is connected with stirring pipe, the centre position of stirring pipe is provided with shaft seat, stirring pipe by shaft seat according in rotating shaft, form T-type structure, stirring pipe is hollow seal pipe, stirring pipe is connected with rotating shaft, one end, termination of stirring pipe is provided with some first pores, another termination of stirring pipe and the opposing side of the first pore are provided with some second pores.Air is passed in the solution in drip chamber by oxygenation rabbling mechanism, increase the oxygen level of solution, passing into air can drive stirring pipe to rotate simultaneously, can stir solution, solution is better mixed with air, increase oxygen level, such solution drips to after on cell battery lead plate, the cell survival on cell battery lead plate can be made better, and the survival life-span is longer, ensure that the accuracy detecting data.
As a kind of preferred version, the pipeline of described rotating shaft is provided with the retaining valve leading to stirring pipe.The gentle body of anti-sealing pours in down a chimney.
As a kind of preferred version, described disk side lower limb is provided with a spacing edge of circle, described drip chamber top is provided with limited block, limited block surface is arcwall face, limited block surface is affixed closely with disk side, limited block on the surface correspondence be provided with stopper slot, spacing edge is embedded in stopper slot.Spacing edge and stopper slot ensure that disk contacts with drip chamber, can not deviation position during disk rotational, and liquid outlet and inlet are aligned.
As a kind of preferred version, described cell battery lead plate is provided with working electrode, to electrode and two contrast electrodes, the front end of described working electrode is circular, this circular portion adopts foam copper to make, and one deck Gold plated Layer is also coated with on these foam coppers, cell is attached on working electrode front end, and the described front end to electrode and contrast electrode is arranged around working electrode front end, and at working electrode, part is coated with one deck painting layer to outside the front end of electrode and contrast electrode.Electrode adopts foam copper to make, and foam copper is equally distributed three-dimensional netted pore structure, and it has the reticulate texture of multi-layer stable and connects closely, and reticulate texture is not yielding and subside.This makes cell can enter foam copper inside configuration, and cell can better be attached on electrode.It is lower that Gold plated Layer compares foam copper toxicity, makes cytoactive better, also make detection sensitivity better with cells contacting.
As a kind of preferred version, described setting on base is provided with electric rotating machine, and described disk is arranged in the rotating shaft of electric rotating machine, is outside equipped with axle sleeve in electric rotating machine rotating shaft, and described drip chamber is fixed on axle sleeve.
Based on a cycloheximide concentration analysis for the biological tongue of surface acoustic wave series resonator, comprise the following steps:
Step one: be placed in by cycloheximide solution in liquid storage cylinder relative with drip chamber in disk, then injects drip chamber by liquid storage luminal solution; Liquid storage cylinder is relative with drip chamber, and namely the liquid outlet of fluid reservoir and the inlet of drip chamber align.
Step 2: by micro pump to dropping liquid on cell battery lead plate, setting each dropping liquid amount is 0.05ml, and dropping liquid interval time is 3.4 seconds, and dropping liquid number of times is 140 times, after dropping liquid terminates, and discharges surplus solution in drip chamber and carry out cleaning to drip chamber to dry up; Open drain solenoid valve, the surplus solution in drip chamber is discharged, after discharge, closes drain solenoid valve.Then open entering water electromagnetic valve and pass into clear water, rinse in drip chamber, open drain solenoid valve after cleaning again and carry out draining.Close entering water electromagnetic valve, open air inlet electromagnetic valve, pass into gas and dry up process in drip chamber, finally close drain solenoid valve.
Step 3: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate exports in the frequency form, converting unit is converted to frequency curve to frequency signal, then be sent on processing unit, processing unit obtains input value S (t) at frequency curve up-sampling; Input value S (t) is substituted into second-order linear system accidental resonance model, this model is:
d 2 x ( t ) dt 2 + [ 4 r 2 + 2 ξ ( t ) + bξ 2 ( t ) ] dx ( t ) dt + ω 4 x ( t ) = A cos ( Ωt ) + cS ( t )
And second-order linearity coefficient accidental resonance model is resonated; Wherein x (t) is vibration particle displacement, and Ω is angular frequency, r and ω is respectively the attenuation coefficient of setting and the frequency of linear oscillator particle, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting 2t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p s(a)=p s(-a)=q, p s(0)=1-2q, wherein 0 < q < 0.5; Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa 2e -λ τ; λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is obtain the output signal-to-noise ratio value of solution
SNR = r&lambda;&Omega; + 2 qa 2 bc ( &Omega; 3 - &Omega; ) 1 + 2 qa 2 b + 5 r - a 2 ;
Step 4: output signal-to-noise ratio value is substituted into cycloheximide solution concentration forecast model:
Con CX = SNR - 553.19 102.87 ,
Calculate the concentration of cycloheximide solution.
As a kind of preferred version, described cycloheximide solution concentration forecast model is calculated by following steps:
Step a: the cycloheximide solution of deployed multiple concentration respectively, is placed in the liquid storage cylinder of disk respectively by cycloheximide solution, inputs the concentration value k of corresponding each cycloheximide solution in processor simultaneously 1, k 2..., k n; N is the quantity detecting solution.
Step b: liquid storage luminal solution relative with drip chamber in disk is injected drip chamber, according to step 2 and three, obtains the output signal-to-noise ratio value of solution;
Step c: rotary disk, makes next liquid storage cylinder relative with drip chamber, repeat step b, like this until on disk all solution all detect, last processor obtain correspondence these solution output signal-to-noise ratio value SNR 1, SNR 2..., SNR n, then according to the solution concentration k of correspondence 1, k 2..., k n, utilize (k 1, SNR 1), (k 2, SNR 2) ..., (k n, SNR n) fit to straight line, draw cycloheximide solution concentration forecast model according to the straight line of matching: Con CX = SNR - 553.19 102.87 .
Therefore, advantage of the present invention is: structure is simple, and cost of manufacture is low, can automatically detect multiple solution concentration, convenient to operation, decreases operating personnel's workload simultaneously, has saved the time.Testing process is convenient and swift, and precision is high.
Accompanying drawing explanation
Accompanying drawing 1 is a kind of structural frames diagram of the present invention;
Accompanying drawing 2 is a kind of structural representations of collecting work platform in the present invention;
Accompanying drawing 3 is a kind of structural representations of oxygenation rabbling mechanism in the present invention;
Accompanying drawing 4 is a kind of structural representations of cell battery lead plate in the present invention.
1-collecting work platform 2-collecting unit 3-converting unit 4-processing unit 5-SAW (Surface Acoustic Wave) resonator 6-cell battery lead plate 7-base 8-disk 9-liquid storage cylinder 10-drip chamber 11-standing groove 12-liquid outlet 13-liquid outlet electromagnetic valve 14-inlet 15-liquid droping head 16-air intake opening 17-air inlet electromagnetic valve 18-water inlet 19-entering water electromagnetic valve 20-freeing port 21-drain solenoid valve 22-electric rotating machine 23-axle sleeve 24-micro pump 25-rotating shaft 26-stirring pipe 27-shaft seat 28-first pore 29-second pore 30-spacing along 31-working electrode 32-to electrode 33-contrast electrode 34-painting layer 35-stopper slot 36-retaining valve
Embodiment
Below by embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.
Embodiment:
A kind of cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator of the present embodiment, includes collecting work platform 1, collecting unit 2, converting unit 3 and processing unit 4.
As shown in Figure 2, collecting work platform includes pedestal 7, and pedestal is vertically provided with electric rotating machine 22, the rotating shaft of electric rotating machine is provided with disk 8, and the rotating shaft of electric rotating machine is outside equipped with axle sleeve 23.Disk is evenly provided with around the center of circle multiple liquid storage cylinder 9 for placing solution, and the bottom of disk side is provided with drip chamber 10, and drip chamber is fixedly connected on axle sleeve, and the top of drip chamber contacts with disk bottom.Disk side lower limb is provided with a circle spacing along 30, drip chamber top is provided with limited block, and limited block surface is arcwall face, and limited block surface is affixed closely with disk side, limited block on the surface correspondence be provided with stopper slot 35, spacing edge is embedded in stopper slot.
The bottom of liquid storage cylinder is provided with liquid outlet 12, liquid outlet is provided with liquid outlet electromagnetic valve 13, on the top of fluid reservoir, corresponding liquid outlet position is provided with inlet 14, and inlet diameter is greater than liquid outlet diameter.The bottom of drip chamber is provided with liquid droping head 15, liquid droping head is provided with micro pump 24, be connected with drip chamber inside by micro pump.
In drip chamber, be provided with wiper mechanism and drainage mechanism, wiper mechanism comprises water inlet 18 and air intake opening 16, and air intake opening and water inlet converge into a pipeline and be then connected with drip chamber inside.Water inlet is provided with entering water electromagnetic valve 19, water inlet is connected with water tank by pipeline, and pipeline is provided with water pump.Air intake opening is provided with air inlet electromagnetic valve 17, air intake opening is connected with air pump.Drainage mechanism comprises the freeing port 20 be arranged on bottom drip chamber, and freeing port is provided with drain solenoid valve 21.
Oxygenation rabbling mechanism is also provided with in drip chamber, as shown in Figure 3, oxygenation rabbling mechanism comprises rotating shaft 25, is provided with pipeline in rotating shaft, and in rotating shaft, pipeline is connected with air intake opening with water inlet, rotating shaft is connected with stirring pipe 26, the centre position of stirring pipe is provided with shaft seat 27, and stirring pipe is arranged in rotating shaft by shaft seat, forms T-type structure, stirring pipe is hollow seal pipe, and stirring pipe is connected with rotating shaft.One end, termination of stirring pipe is provided with some first pores 28, another termination of stirring pipe and the opposing side of the first pore are provided with some second pores 29.The pipeline of rotating shaft is provided with the retaining valve 36 leading to stirring pipe.
Liquid droping head lower base is provided with standing groove 11, bottom standing groove, is provided with water delivering orifice.
Collecting unit comprises cell battery lead plate 6 and SAW (Surface Acoustic Wave) resonator 5, on the bag resonator of cell battery lead plate connection sound surface.During operation, cell battery lead plate is placed in the standing groove of collecting work platform.As shown in Figure 1, SAW (Surface Acoustic Wave) resonator is connected with converting unit, and converting unit is connected with processing unit, and SAW (Surface Acoustic Wave) resonator is connected with power supply.As shown in Figure 4, cell battery lead plate is provided with working electrode 31, to electrode 32 and two contrast electrodes 33.The front end of working electrode is circular, this circular portion adopts foam copper to make, and one deck Gold plated Layer is also coated with on these foam coppers, cell is attached on working electrode front end, the front end of electrode and contrast electrode is arranged around working electrode front end, and at working electrode, part is coated with one deck painting layer 34 to outside the front end of electrode and contrast electrode.
Based on the cycloheximide concentration analysis of the biological tongue of surface acoustic wave series resonator, comprise the following steps:
Step one: be placed in by cycloheximide solution in liquid storage cylinder relative with drip chamber in disk, then injects drip chamber by liquid storage luminal solution.
Step 2: by micro pump to dropping liquid on cell battery lead plate, setting each dropping liquid amount is 0.05ml, and dropping liquid interval time is 3.4 seconds, and dropping liquid number of times is 140 times.After dropping liquid terminates, open drain solenoid valve, the surplus solution in drip chamber is discharged, after discharge, closes drain solenoid valve.Then open entering water electromagnetic valve and pass into clear water, rinse in drip chamber, open drain solenoid valve after cleaning again and carry out draining.Close entering water electromagnetic valve, open air inlet electromagnetic valve, pass into gas and dry up process in drip chamber, finally close drain solenoid valve.
Step 3: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate exports in the frequency form, converting unit is converted to frequency curve to frequency signal, then be sent on processing unit, processing unit obtains input value S (t) at frequency curve up-sampling; Input value S (t) is substituted into second-order linear system accidental resonance model, this model is:
d 2 x ( t ) dt 2 + [ 4 r 2 + 2 &xi; ( t ) + b&xi; 2 ( t ) ] dx ( t ) dt + &omega; 4 x ( t ) = A cos ( &Omega;t ) + cS ( t )
And second-order linearity coefficient accidental resonance model is resonated; Wherein x (t) is vibration particle displacement, and Ω is angular frequency, r and ω is respectively the attenuation coefficient of setting and the frequency of linear oscillator particle, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting 2t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p s(a)=p s(-a)=q, p s(0)=1-2q, wherein 0 < q < 0.5; Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa 2e -λ τ; λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is obtain the output signal-to-noise ratio value of solution
SNR = r&lambda;&Omega; + 2 qa 2 bc ( &Omega; 3 - &Omega; ) 1 + 2 qa 2 b + 5 r - a 2 ;
Step 4: output signal-to-noise ratio value is substituted into cycloheximide solution concentration forecast model:
Con CX = SNR - 553.19 102.87 ,
Calculate the concentration of cycloheximide solution.
Wherein cycloheximide solution concentration forecast model is calculated by following steps:
Step a: the cycloheximide solution of deployed multiple concentration respectively, is placed in the liquid storage cylinder of disk respectively by cycloheximide solution, inputs the concentration value k of corresponding each cycloheximide solution in processor simultaneously 1, k 2..., k n;
Step b: liquid storage luminal solution relative with drip chamber in disk is injected drip chamber, according to step 2 and three, obtains the output signal-to-noise ratio value of solution;
Step c: rotary disk, makes next liquid storage cylinder relative with drip chamber, repeat step b, like this until on disk all solution all detect, last processor obtain correspondence these solution output signal-to-noise ratio value SNR 1, SNR 2..., SNR n, then according to the solution concentration k of correspondence 1, k 2..., k n, utilize (k 1, SNR 1), (k 2, SNR 2) ..., (k n, SNR n) fit to straight line, draw cycloheximide solution concentration forecast model according to the straight line of matching: Con CX = SNR - 553.19 102.87 .
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.
Although more employ the terms such as collecting work platform, collecting unit, converting unit, processing unit herein, do not get rid of the possibility using other term.These terms are used to be only used to describe and explain essence of the present invention more easily; The restriction that they are construed to any one additional is all contrary with spirit of the present invention.

Claims (10)

1. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator, it is characterized in that: comprise collecting work platform (1), collecting unit (2), converting unit (3) and processing unit (4), described collecting work platform comprises pedestal (7), on pedestal, rotary setting has disk (8), disk is provided with multiple liquid storage cylinder (9) around the center of circle, disk one side lower part is provided with drip chamber (10), described drip chamber top contacts with disk bottom, liquid outlet (12) is provided with bottom liquid storage cylinder, liquid outlet is provided with liquid outlet electromagnetic valve (13), at drip chamber top, corresponding liquid outlet position is provided with inlet (14), liquid droping head (15) is provided with on bottom described drip chamber, wiper mechanism and drainage mechanism is provided with in drip chamber, the below pedestal of liquid droping head is provided with standing groove (11), described collecting unit comprises the cell battery lead plate (6) and SAW (Surface Acoustic Wave) resonator (5) that are connected, cell battery lead plate is located in standing groove, described SAW (Surface Acoustic Wave) resonator is connected with converting unit, converting unit is connected with processing unit.
2. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 1, it is characterized in that described wiper mechanism comprises the water inlet (18) and air intake opening (16) be communicated with drip chamber inside, water inlet is connected with water tank, air intake opening is connected with air pump, water inlet and air intake opening are respectively arranged with entering water electromagnetic valve (19) and air inlet electromagnetic valve (17), described drainage mechanism comprises the freeing port (20) be arranged on bottom drip chamber, and freeing port is provided with drain solenoid valve (21).
3. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 1, is characterized in that described liquid droping head (15) is connected with drip chamber (10) by micro pump (24).
4. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 2, it is characterized in that being provided with oxygenation rabbling mechanism in drip chamber (10), oxygenation rabbling mechanism comprises rotating shaft (25), pipeline is provided with in rotating shaft, described rotating shaft is connected with air intake opening (16) with water inlet (18), rotating shaft is connected with stirring pipe (26), the centre position of stirring pipe is provided with shaft seat (27), stirring pipe is arranged in rotating shaft by shaft seat, form T-type structure, stirring pipe is hollow seal pipe, stirring pipe is connected with rotating shaft, one end, termination of stirring pipe is provided with some first pores (28), another termination of stirring pipe and the opposing side of the first pore are provided with some second pores (29).
5. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 4, is characterized in that on the pipeline of described rotating shaft (25), be provided with the retaining valve (36) leading to stirring pipe.
6. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 1 or 2 or 3 or 4 or 5, it is characterized in that described disk (8) side lower limb is provided with a spacing edge of circle (30), described drip chamber (10) top is provided with limited block, limited block surface is arcwall face, limited block surface is affixed closely with disk side, limited block on the surface correspondence be provided with stopper slot (35), spacing edge is embedded in stopper slot.
7. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 1 or 2 or 3 or 4 or 5, it is characterized in that being provided with working electrode (31) on described cell battery lead plate (6), to electrode (32) and two contrast electrodes (33), the front end of described working electrode is circular, this circular portion adopts foam copper to make, and one deck Gold plated Layer is also coated with on these foam coppers, cell is attached on working electrode front end, the described front end to electrode and contrast electrode is arranged around working electrode front end, and at working electrode, to outside the front end of electrode and contrast electrode, part is coated with one deck painting layer (34).
8. the cycloheximide concentration analysis device based on the biological tongue of surface acoustic wave series resonator according to claim 1 or 2 or 3 or 4 or 5, described in it is characterized in that, the upper setting of base (7) is provided with electric rotating machine (22), described disk is arranged in the rotating shaft of electric rotating machine, be outside equipped with axle sleeve (23) in electric rotating machine rotating shaft, described drip chamber is fixed on axle sleeve.
9., based on a cycloheximide concentration analysis for the biological tongue of surface acoustic wave series resonator, adopt the device in any one of power 1-8, it is characterized in that comprising the following steps:
Step one: be placed in by cycloheximide solution in liquid storage cylinder relative with drip chamber in disk, then injects drip chamber by liquid storage luminal solution;
Step 2: by micro pump to dropping liquid on cell battery lead plate, setting each dropping liquid amount is 0.05ml, and dropping liquid interval time is 3.4 seconds, and dropping liquid number of times is 140 times, after dropping liquid terminates, and discharges surplus solution in drip chamber and carry out cleaning to drip chamber to dry up;
Step 3: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate exports in the frequency form, converting unit is converted to frequency curve to frequency signal, then be sent on processing unit, processing unit obtains input value S (t) at frequency curve up-sampling; Input value S (t) is substituted into second-order linear system accidental resonance model, this model is:
d 2 x ( t ) dt 2 + [ 4 r 2 + 2 &xi; ( t ) + b&xi; 2 ( t ) ] dx ( t ) dt + &omega; 4 x ( t ) = A cos ( &Omega;t ) + cS ( t )
And second-order linearity coefficient accidental resonance model is resonated; Wherein x (t) is vibration particle displacement, and Ω is angular frequency, r and ω is respectively the attenuation coefficient of setting and the frequency of linear oscillator particle, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting 2t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p s(a)=p s(-a)=q, p s(0)=1-2q, wherein 0 < q < 0.5; Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa 2e -λ τ; λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is obtain the output signal-to-noise ratio value of solution
SNR = r&lambda;&Omega; + 2 qa 2 bc ( &Omega; 3 - &Omega; ) 1 + 2 qa 2 b + 5 r - a 2 ;
Step 4: output signal-to-noise ratio value is substituted into cycloheximide solution concentration forecast model:
Con CX = SNR - 553.19 102.87 ,
Calculate the concentration of cycloheximide solution.
10. the cycloheximide concentration analysis based on the biological tongue of surface acoustic wave series resonator according to claim 9, is characterized in that described cycloheximide solution concentration forecast model is calculated by following steps:
Step a: the cycloheximide solution of deployed multiple concentration respectively, is placed in the liquid storage cylinder of disk respectively by cycloheximide solution, inputs the concentration value k of corresponding each cycloheximide solution in processor simultaneously 1, k 2..., k n;
Step b: liquid storage luminal solution relative with drip chamber in disk is injected drip chamber, according to step 2 and three, obtains the output signal-to-noise ratio value of solution;
Step c: rotary disk, makes next liquid storage cylinder relative with drip chamber, repeat step b, like this until on disk all solution all detect, last processor obtain correspondence these solution output signal-to-noise ratio value SNR 1, SNR 2..., SNR n, then according to the solution concentration k of correspondence 1, k 2..., k n, utilize (k 1, SNR 1), (k 2, SNR 2) ..., (k n, SNR n) fit to straight line, draw cycloheximide solution concentration forecast model according to the straight line of matching: Con CX = SNR - 553.19 102.87 .
CN201510067409.XA 2015-02-09 2015-02-09 Cycloheximide concentration analysis device and method based on surface acoustic wave series resonator biology tongue Expired - Fee Related CN105021670B (en)

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