CN104964973A - Test paper reading and analyzing method and system based on mobile terminal camera - Google Patents
Test paper reading and analyzing method and system based on mobile terminal camera Download PDFInfo
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- CN104964973A CN104964973A CN201510397181.0A CN201510397181A CN104964973A CN 104964973 A CN104964973 A CN 104964973A CN 201510397181 A CN201510397181 A CN 201510397181A CN 104964973 A CN104964973 A CN 104964973A
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Abstract
The invention discloses a test paper reading and analyzing method and system based on a mobile terminal camera, which is used for calculating a concentration value of a to-be-detected substance in a detection sample and analyzing a test result. The method comprises the following steps: collecting, by the mobile terminal camera, a test paper image, analyzing and identifying the edge of a color developing area, and intercepting a required color development area; calculating a color value or a gray value of a test area (T line) and a control area (C line) in the color development area picture, and calculating the concentration ratio of the sample by utilizing the ratio of the color value or the gray value of the test area (T line) and the control area (C line); calibrating the sample concentration ratio in batches, substituting the sample concentration ratio into a standard curve of the test paper of the batch to perform the fitting operation, and calculating the real concentration value of the to-be-detected substance in the sample; looking for a matched detection result according to the real concentration value, and transmitting the detection result to the mobile terminal.
Description
Technical field
The invention belongs to test paper to read and analysis field, particularly a kind of test paper based on mobile terminal camera and cloud server reads and analytical approach and system.
Background technology
Test paper is widely used in the pattern detection of numerous areas, such as, in clinical treatment conventional routine urinalysis detection, prenatal and postnatal care, infertile detection, formaldehyde in environment measuring, the content detection of benzene, and clenbuterol hydrochloride, melamine, waste oil or the Detecting Pesticide etc. in food security, all need to use corresponding special test paper to detect.
Some simple test paper inspections can be realized by visual inspection test paper, such as very early pregnancy test paper, but this mode by range estimation test paper can only detect qualitatively, and it is lower if there is detected object content, when developing the color more shallow, easily occur misreading, visual inspection is also subject to the interference of many artificial or environmental factors such as mood, light in addition, thus affects its accuracy.Particularly some even cannot use such test paper to detect to the insensitive crowd of particular color.
Need the test paper carrying out quantitatively detecting normally to be come by distinctive equipment in professional institution, also have some detections to need the result comprehensively repeatedly checked, the micro-judgment relying on professional just can complete.The morning of such as ectopic pregnancy finds for protection female fertility function, prevents habitual abortion etc. to be all very helpful; but the preventive inspection of ectopic pregnancy needs repetitive measurement HCG concentration; if HCG concentration doubled above in every 48 hours; be inferred as normal uterine pregnancy; if be starkly lower than one times of concentration, ectopic pregnancy or hypogenetic possibility increase; such pregnant woman repeatedly need arrive the detection that hospital carries out HCG concentration, brings many fatigues and the risk contacting nosocomialtion.But current existing Test paper also cannot be accomplished to detect in family, is limited to the restriction of family's quantitative measurement technology exactly.
Even the Test paper in professional institution, for the test paper of different batches, if do not have supporting good batch calibration card, its assay just has differences between batches, also can have influence on the accuracy of testing result.And the detection usefulness of immune detection product also can produce certain decay after dispatching from the factory along with the prolongation of time, even if traditional testing product is furnished with the calibration card of subsidiary calibration curve when dispatching from the factory, but cannot accomplish to carry out time dependent real-time update to calibration curve.
Summary of the invention
(1) technical matters that will solve
The technical problem to be solved in the present invention is: in existing test paper detecting method, the accuracy of artificial judgment, poor stability, cannot quantitatively judge, and machine judges to need professional equipment, also likely need the micro-judgment relying on professional, detection must be completed in special environment.
Another problem that the present invention will solve there is difference between batch between the test paper of different batches, have impact on the accuracy of measurement.Traditional testing product also can occur to decay along with time variations usefulness thus affect the problem of measurement accuracy.
The object of this invention is to provide a kind of test paper based on mobile terminal camera and cloud server to read and analytical approach and system, the quantitative and qualitative analysis making user that existing mobile terminal and cloud server can be utilized to complete test paper detects, at home or open air can complete some test items that originally only just can complete in hospital or special environment, thus realize the function of mobile telemedicine, mobile monitoring.
Another object of the present invention is to provide a kind of test paper based on mobile terminal camera and cloud server and reads and analytical approach and system, eliminates the difference between batch of test paper different batches, and improves the accuracy of detection paper.
(2) technical scheme
The invention provides a kind of test paper based on mobile terminal camera and cloud server to read and analytical approach, for calculating the concentration value of sample in test paper and analytical test result, comprise the following steps: mobile terminal camera gathers test paper image, analyze the edge identifying colour developing district, intercept satisfactory color development area, send to cloud server; Calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio; To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample; Search the testing result of coupling according to the actual concentration value of thing to be checked in sample, and testing result is sent to mobile terminal.
Preferably, before mobile terminal camera gathers test paper image, first camera gathers the identification code of test paper, and identification code is sent to cloud server, cloud server call from database detection paper project, batch etc. information temporary storage return to mobile terminal.
Optionally, before mobile terminal camera gathers test paper image, prompting user, in manual classification, the batch information selecting input test paper of mobile terminal, sends to cloud server.
Preferably, mobile terminal camera gathers the image of whole test strips; Color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, find the frame preset, this region selected; Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
In another kind of preferred mode, mobile terminal camera gathers the image of whole test strips; The position of screening anchor point, by the position of the anchor point position of color development area, location and level, vertical direction; Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
Optionally, for the common test paper of third party's Non-precondition frame, have the view-finder meeting test paper size on a display screen, prompting user entangles whole test paper or color development area with view-finder, and clicks button of taking pictures.
Preferably, mobile terminal carries out standardization to the colour developing district picture intercepted, and photo finishing is become unified pixel size, or is returned by the picture rotation offset on time space of taking pictures or in plane.
Preferably, beyond the clouds in server, if the color value of reference area (C line) or gray-scale value are β, color value or the gray-scale value of test section (T line) are α, obtain concentration of specimens ratio γ by the relevant ratio computing of α and β.
For nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color value or gray-scale value, as with reference to district's color value or gray-scale value β.
Cloud server carries out a batch step for calibration and comprises: corresponding every series-produced test paper product, use normal concentration material to calibrate this batch products, draw concentration of specimens ratio by the ratio computing of test section (T line) and control zone (C line) both color values or gray-scale value; According to the corresponding relation of concentration of specimens ratio and standard substance actual concentration value, produce the typical curve of this batch of test paper, and this typical curve is kept in the database of cloud server; The time dependent change of product that cloud server keeps sample according to stock, upgrades typical curve; According to the colour developing image of mobile terminal transmission, the concentration of specimens calculated ratio (C, T ratio), carries out fitting operation with the typical curve of this batch, draws this actual concentration value checked.
Another preferred embodiment in, cloud server also needs to judge that this test item is the need of again detecting; If without the need to again detecting, then the numerical range in the actual concentration value of thing to be checked in the sample calculated and database being compared, finding out diagnostic result corresponding to this concentration value and corresponding medical advice; Preserve sample values and corresponding testing result, send testing result, corresponding diagnosis and suggestion to mobile terminal; If need to carry out repeated detection, then preserve this sample actual concentration value, send to mobile terminal and detect sample results and detection time next time; Timer sets the time next time detected, and sends prompting message when timer arrives to mobile terminal, and receives the test paper picture next time detected.
The present invention also provides a kind of test paper based on mobile terminal camera and cloud server to read and analytic system, for calculating the concentration value of sample in test paper and analytical test result, comprise: mobile terminal, for gathering test paper image, analyze the edge identifying colour developing district, intercept satisfactory color development area, send to cloud server; Cloud server, for receiving and analyze colour developing district picture, calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio; To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample; Search the testing result of coupling according to the actual concentration value of thing to be checked in sample, and testing result is sent to mobile terminal.
Test paper for testing comprises immune chromatography test paper or chemical colour reaction test paper, described test paper comprises identification code, shown in identification code comprise Quick Response Code, bar code or other coded systems for identifying, for distinguishing type and the batch information of test paper, be positioned at the optional position on test strips and shell.
Preferably, first mobile terminal gathers the identification code of test paper, identification code is sent to cloud server before gathering test paper image, cloud server call from database detection paper project, batch etc. information temporary storage return to mobile terminal.
Optionally, before mobile terminal gathers test paper image, prompting user, in manual classification, the batch information selecting input test paper of mobile terminal, sends to cloud server.
Preferably, mobile terminal also comprises: camera, for gathering the image of whole test strips; Processing unit, for color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, finds the frame preset, this region selected; Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out; Transmission and reception unit, for sending to cloud server by the color development area image of intercepting.
Preferably, mobile terminal also comprises: camera, for gathering the image of whole test strips; Processing unit, for screening the position of anchor point, by the position of the anchor point position of color development area, location and level, vertical direction; Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out; Transmission and reception unit, for sending to cloud server by the color development area image of intercepting.
Mobile terminal also comprises: touch display screen, for the common test paper for third party's Non-precondition frame, on touch display screen, display meets the view-finder of test paper size, and prompting user entangles whole test paper or color development area with view-finder, and clicks button of taking pictures.
Preferably, mobile terminal carries out standardization to the colour developing district picture intercepted, and photo finishing is become unified pixel size, or is returned by the picture rotation offset on time space of taking pictures or in plane.
Cloud server also comprises processing unit, and for setting the color value of reference area (C line) or gray-scale value as β, color value or the gray-scale value of test section (T line) are α, then obtain concentration of specimens by the relevant ratio computing of α and β and compare γ.
For nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color value or gray-scale value, as with reference to district's color value or gray-scale value β.
Preferably, cloud server also comprises database, for storing the typical curve of each batch of test paper, its standard curve is corresponding every series-produced test paper product, normal concentration material is used to calibrate this batch products, concentration of specimens ratio is drawn by the ratio computing of test section (T line) and control zone (C line) both color values or gray-scale value, according to the corresponding relation of concentration of specimens ratio and standard substance actual concentration value, produce the typical curve of this batch of test paper, and this typical curve is kept in the database of cloud server; Processing unit, according to the time dependent change of product that stock keeps sample, upgrades typical curve; According to the colour developing image of mobile terminal transmission, the concentration of specimens ratio calculated (C, T ratio), carries out fitting operation with the typical curve of this batch, draws this actual concentration value checked.
The present invention also provides a kind of test paper based on mobile terminal camera to read and analytic system, for calculating the concentration value of sample in test paper and analytical test result, comprise: mobile terminal, for gathering test paper image, analyze the edge identifying colour developing district, intercept satisfactory color development area, send to cloud server; Cloud server, for the colour developing district picture according to reception, calculate color value or the gray-scale value of test section (T line) and control zone (C line) in colour developing district picture, the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio; To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample; Judge that this test item is the need of again detecting; If without the need to again detecting, then the numerical range in the sample actual concentration value of calculating and database being compared, finding out diagnostic result corresponding to this concentration value and corresponding medical advice; Preserve sample values and cure diagnostic result accordingly, returning testing result, corresponding diagnosis and suggestion to user; If need to carry out repeated detection, then preserve this sample actual concentration value, send to user and detect sample results and detection time next time; Timer sets the time next time detected, and sends prompting message when timer arrives to the mobile terminal of user, receives the colour developing district picture next time detected.
The present invention also provides a kind of test paper based on mobile terminal camera and cloud server to read and analytic system, for calculating the concentration value of sample in test paper and analytical test result, comprising: mobile terminal, for gathering test paper image, sending to cloud server; Cloud server, for receiving test paper picture, analyze the edge identifying colour developing district, intercept satisfactory color development area, calculate color value or the gray-scale value of test section (T line) and control zone (C line) in colour developing district, the ratio of both utilizations color value or gray-scale value draws concentration of specimens ratio; To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample; Search the testing result of coupling according to the actual concentration value of thing to be checked in sample, and testing result is sent to mobile terminal.
The present invention is also a kind of to read and the mobile terminal analyzed and method for test paper, comprising: camera, for obtaining identification code and the image of test paper; Touch display screen, for showing the picture material of acquisition, prompting user aims at the tram of test paper; Processing unit, for analyzing the edge identifying colour developing district, intercepts satisfactory color development area; Transmission and reception unit, for sending the message that identification code and colour developing district's picture and reception cloud server send to cloud server.
The present invention also provides a kind of and reads and the cloud server analyzed and method for test paper, comprising: sending/receiving module, for receiving test paper color development district picture, and sends testing result to mobile terminal; Database, for storing the typical curve of each test item different batches, described typical curve is for representing the corresponding relation of measured value and actual concentration value, and diagnostic result corresponding to the actual concentration value storing different test item and medical advice; Processing unit, for analyzing colour developing district picture, calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio; To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample; The testing result of coupling is searched according to the actual concentration value of thing to be checked in sample.
(3) beneficial effect
Test paper based on mobile terminal camera and cloud server of the present invention reads and analytical approach and system, relative to visual inspection judged result, user not only can obtain the qualitative results of test paper more accurately, quantitative detection can also be realized, and without the need to relying on professional equipment, more can obtain diagnostic result and the suggestion of specialty, cloud server can carry out a batch calibration to test paper in addition, and measurement result is more accurate.User can set up oneself health account database, is kept at for a long time in cloud server, is convenient to carry out long-term dynamics observation to result.
Accompanying drawing explanation
Fig. 1 is that the test paper based on mobile terminal camera of the present invention reads the schematic diagram with analytic system.
Fig. 2-1-2-3 is the schematic diagram that the present invention uses test paper.
Fig. 3 is the structural representation of mobile terminal of the present invention.
Fig. 4 is the schematic diagram of acquisition for mobile terminal test paper image of the present invention and analysis test paper image.
Fig. 5 is the structural representation of cloud server of the present invention.
Fig. 6 is the exemplary plot of the typical curve stored in cloud server of the present invention.
Fig. 7 is the process flow diagram that cloud server of the present invention calculates test paper image and result judges.
Fig. 8 is the process flow diagram of another embodiment that cloud server of the present invention calculates test paper image and result judges.
Fig. 9 is that cloud server of the present invention carries out a batch process flow diagram for calibration to test paper.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in further detail.
As shown in Figure 1, system of the present invention comprises test paper 1, mobile terminal 2, cloud server 3 and user 4.First the camera 301 of mobile terminal 2 gathers the identification code (step 101) of test paper 1, identification code is sent to cloud server 3 (step 102), cloud server 3 calls detection paper project from database, batch etc. information return to mobile terminal (step 103), mobile terminal 2 gathers colour developing district picture (step 104) of test paper, and colour developing district picture is sent to cloud server 3 (step 105), cloud server calculates the concentration value of sample according to the colour developing district picture received, draw testing result, mobile terminal 2 feedback test result to user 4 advises and stores detecting data (step 106) with specialized.Optionally, cloud server also comprises the function of cloud data analysis, to the data analysis detected, certain a period of time disease in such as a certain area is occurred frequently, or certain residue of veterinary drug exceeds standard early warning etc., also has customer service system, doctor and the consulting service of expert's online direction etc.
The test paper that the present invention adopts can be that immune chromatography test paper, chemical colour reaction test paper etc. can the test paper of Show Color feature.The structure of test paper 1 as shown in Figure 2.Fig. 2-1 is an example of immune chromatography test paper, and control colour developing bar C line display standard vitta, C line represents control zone.T line represents test section, and detect the concentration of material to be checked in colour developing bar T line reaction sample, the depth of T line color and the proportional relation of concentration of material to be checked, in the darker representative sample of color, the concentration of thing to be checked is higher.Extraneous ambient light has a lot of difference, but T line and C line are all under being in same ambient light, and the color depth of C line representative is standard color depth, and C line represents control zone, thus accurately can draw the color depth of T line as a reference with C line.Concrete concentration of specimens quantivative approach elaborates in the introduction hereafter about cloud server 3.Well S is for placing tested sample.Optionally, some test paper can not have well S yet, can be inhale lining bar or can also by sample directly as colour developing district.Identification code 201 can be Quick Response Code, bar code or other coded systems for identifying, and for distinguishing type and the batch information of test paper, identification code can be positioned at the optional position on test strips and shell.In addition, optionally, Quick Response Code may reside on examination bar, in packaging bag, on packing box, or in certain annex.Can be positioned at as shown in Fig. 2-1 above colour developing bar, but be not limited thereto, it will be understood by those skilled in the art that identification code also can be positioned at below colour developing bar or optional position, side.As shown in accompanying drawing 2-1, be color development area in dotted line frame 202, frame can be the color preset, and also by adopting different colours by inside and outside for frame 202, can be distinguished by aberration.
In another optional scheme, test paper or shell are provided with anchor point, for locating the colour developing district of test paper.Anchor point can be spraying or directly be produced on test paper or shell, can be one, two or more.Camera is caught this anchor point and is positioned colour developing frame with the coordinate system that this anchor point is formed.
In addition, test paper of the present invention also can adopt other nonimmune chromatographic test papers, as shown in Fig. 2-2 and 2-3, Fig. 2-2 is urine ten test paper, Fig. 2-3 is other chemical test paper, the place that this type of test paper is identical with chromatographic test paper is all mode based on color reaction, and difference is that chemical test paper does not have C line as a reference, but adopt the color compensating of other form blank pad etc. to be used as reference.
The structure of mobile terminal 2 as shown in Figure 3.Mobile terminal 2 comprises camera 301, touch display screen 302, transmission and reception unit 303, processing unit 304.Camera 301 is for obtaining identification code and the image of test paper 1, touch display screen 302 can show the picture material of acquisition, user is and guided to aim at the tram of test paper, processing unit 304 is for controlling camera 301 and touch-screen display 302 obtains test paper image, analyze the edge identifying colour developing district, intercept satisfactory color development area, transmission and reception unit 303 is for sending colour developing district picture, userspersonal information etc. and receiving the message of cloud server 3 transmission to cloud server 3.User can check each testing result and the health account of oneself at any time by the touch display screen 302 of mobile terminal.
Fig. 4 illustrate the embodiment that mobile terminal 2 obtains test paper image and analysis test paper image.As shown in Figure 4, in step 401, camera 301 scans the identification code of test paper, and the information of identification code of acquisition is sent to cloud server 3.In step 402, mobile terminal 2 receive cloud server return detection paper project, batch or the information such as calibration curve.
Another can in alternate embodiments in, for the common test paper not having identification code that third party provides, the touch display screen 302 of mobile terminal can point out user to select test paper type, test item, display screen 302 can provide multiple choice box, user manually selects the type of test paper, input batch information, and the test paper type that user selects is sent to cloud server 3.
In step 403, the image of the whole test strips of camera collection.
In step 404, processing unit, by color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, finds the frame preset, selected this region, i.e. the colour developing district frame 202 of test paper.For the embodiment of test paper being provided with anchor point, by anchor point pixel each other, the distance proportion relation preset, in the process of scanning, remove the identification point of interference and mistake, filter out the position of anchor point.Because anchor point is relative with the position of result determinating area fixing, so accurately can be oriented the position of the position of result determinating area and level, vertical direction by anchor point.
In step 405, find out the color development area meeting proportion requirement in this region, and intercept out.It is no matter the recognizer by anchor point, or to be got stuck by test paper and the algorithm of color distinction between color development area, these two kinds of limb recognition algorithms, make test paper, make plastics get stuck or print make anchor point time, all can set ratio in advance, acquisition image time, as long as have found the proportionate relationship parameter meeting known setting, also just mean and determine object color development area, based on above-mentioned two algorithms, object color development area can both be determined accurately.
For the common test paper of third party's Non-precondition frame, there is the view-finder meeting test paper size on a display screen, user needs to entangle whole test paper or color development area with view-finder, trigger automatic camera or click to take pictures button, mobile terminal can eject after taking pictures the edge that identifies automatically according to above-mentioned edge algorithms picture and identified cut out frame, then whether limb recognition is correct to require user to confirm by hand, can pull if incorrect the edge cutting out frame by hand to revise, then confirmation is clicked, then mobile terminal starts the analysis and calculation carrying out color development area in image, finally return user's result.
In order to increase the accuracy of detection, optionally, in step 406, mobile terminal also carries out standardization to the picture intercepted, effective result determinating area of picture is intercepted out, be organized into unified pixel size simultaneously, also comprise the analysis by algorithm, the picture rotation offset on time space of taking pictures or in plane is returned.
Optionally, step 404-406 also can complete at server end, namely sends to cloud server, by server completing steps 404-406 after the whole test strips image of step 403 camera collection.
The structure of cloud server as shown in Figure 5.Sending/receiving module 501 is for sending and receiving information with mobile terminal.Processing unit 502 is for the function such as analysis chart picture, numerical evaluation, batch calibration, result coupling.Database 503 stores the typical curve of each test item different batches, namely the concentration of specimens ratio measured and the corresponding relation of actual concentration value, simultaneously, database 503 also stored for diagnostic result corresponding to the concentration value of different test item and medical advice, and for the concentration of specimens value that user measures at every turn, a health account is set up to each user.
Fig. 6 shows the test paper standard curve synoptic diagram of some batch stored in database 503.In diagnostic reagent industry, raw material ratio used, as antibody etc., is biological active matter, has active etc. difference, so there is difference between batch between each batch between often criticizing.So-called typical curve, the namely corresponding relation of measured value and actual concentration value.Be illustrated in figure 6 the test paper standard curve synoptic diagram of certain batch, X direction represents actual concentration value x, y direction representative sample concentration ratio γ, cloud server calculates concentration of specimens ratio γ 1, γ 2 etc., γ 1, γ 2 is substituted in typical curve and just correspondence can find actual concentration value x1 and x2 etc.
With reference to figure 7, the method step that cloud server of the present invention 3 pairs of test paper images calculate and result judges is described.In step 701, the test paper identifying information that cloud server mobile terminal receive sends and colour developing picture.In step 702, color, gray analysis are carried out to colour developing picture, changes into corresponding numerical value.In step 703, utilize and change into the C line of color value or gray-scale value and the value of T line, calculate sample content.If the color value of C line or gray-scale value are the color value of β, T line or gray-scale value is α, then obtains concentration of specimens by the relevant ratio computing of α and β and compare γ.According to different test item situations, concrete compute mode is different.Such as, this computing can be α/β or beta/alpha, α 2/ β 2, (α 2+5)/(β 2+6) etc., its core feature, for including ratio computing in this computing, eliminates by this ratio computing the skew that environmental factor causes to a certain extent.
For non-chromatographic test paper, it is the color change depending on final chemical test paper conversion zone that result judges, so it does not possess the concept of C line and T line, in such an embodiment, by its Standard Colors blank pad or take edge, get stuck, the image etc. of external packing, as C line calibration color value or gray-scale value β.As shown in Fig. 2-2, comprise be not limited to two color lumps zone line, test paper bottom blank parts, the test paper plastics substitute that the region such as part, a test paper external packing part gathers as C line that gets stuck participates in calculating.By the color value of colour developing color lump and blank parts or gray-scale value are carried out ratio budget, calculate concentration of specimens ratio further.
In step 704, the typical curve concentration of specimens ratio γ obtained being substituted into measurement result and actual concentration in this test item and batch products stored in server database 503 carries out the Fitting Calculation, draws the sample actual concentration value of this detection.
In step 705, the numerical range in the actual concentration value of the thing to be checked calculated and database is compared, find out diagnostic result corresponding to this concentration value and corresponding medical advice.Or for non-medical test item, in database, also store judged result corresponding to concentration value, such as whether content of formaldehyde exceed standard, the judged result such as persticide residue grade, find out the differentiation result that this concentration value is corresponding.
In step 706, preserve the actual concentration value of thing to be checked and differentiate result accordingly, sending testing result, corresponding diagnosis and suggestion to mobile terminal.
In another interchangeable embodiment, step 702-704 can realize in the terminal, the identification code of mobile scanning terminal test paper, after identification code is sent to cloud server, cloud server returns the test paper type of identification code mark, batch and typical curve, mobile terminal gathers test paper image, analyze colour developing area edge and intercept color development area (see above to the description of accompanying drawing 4), do not need to send to cloud server again, but direct completing steps 702-704, i.e. computation and measurement value and to carry out batch calibration in the terminal, and calculate the concentration values of detection paper thing to be checked, carry out subsequent treatment again.Such as, mobile terminal can directly complete follow-up step 705-706, also again the concentration value of the test paper of calculating can be sent to cloud server, by cloud server completing steps 705-706.
Fig. 8 is another embodiment that cloud server of the present invention 3 pairs of test paper images calculate and result judges.Wherein, step 801-804 is identical with the 701-704 step in accompanying drawing 7.In step 805, judge that this test item is the need of again detecting, if without the need to again detecting, then enter step 806, the concentration of specimens of calculating is compared than with the numerical range in database, finds out diagnostic result corresponding to this content value and corresponding medical advice.In step 807, preserve sample values and cure diagnostic result accordingly, returning testing result, corresponding diagnosis and suggestion to user.If need to carry out repeated detection, then enter step 808, preserve this sample values, send to mobile terminal and detect sample results and detection time next time.In step 809, timer sets the time next time detected, and sends prompting message when timer arrives to the mobile terminal of user, and turns back to step 801.
With reference to figure 9, illustrate that cloud server of the present invention 3 pairs of test paper carry out a batch method for calibration.In step 901, corresponding every series-produced test paper product, uses normal concentration material to calibrate this batch products, draws the corresponding relation of C, T ratio and standard substance concentration, produce shown in canonical plotting Fig. 6, and this typical curve is kept in the database 503 of cloud server.In step 902, the time dependent change of product that cloud server keeps sample according to stock, timely replacement typical curve.In step 903, according to the colour developing image of customer mobile terminal transmission, the concentration of specimens calculated ratio (C, T ratio), carries out fitting operation with typical curve, draws the actual concentration value of this inspection.By method of the present invention, owing to storing the typical curve of product of each batch in cloud server, thus eliminate the difference between batch of different batches product, the measurement result obtained is more accurate.Because of the present invention batch of calibration curve is stored on cloud server, and the renewal of calibration curve can be carried out at any time according to the time variations of the product that keeps sample, eliminate the difference between batch of testing product to the full extent, ensure that the accuracy of testing result.
Be detected as example with ectopic pregnancy, mobile terminal knows the project of this detection by scanning recognition code or manual input-mode, and points out user to use urina sanguinis to test in correct time range.After first time, urina sanguinis measured, colour developing picture is sent to cloud server by mobile terminal, cloud server receives the colour developing picture of test paper, carry out batch calibrating and calculate the HCG concentration value of first time urina sanguinis sample, judge also to need to detect next time, return HCG testing result and information to user " please to get urina sanguinis morning day at * * and continue test ", prompting user tests after 48 hrs again, cloud server arranges timer, user again examines when within 48 hours, arriving and uses urina sanguinis to test, the step of once testing before repetition, cloud server receives the picture that develops the color for the second time, calculate HCG concentration, calculate HCG concentration, judge whether also to need to detect again, according to the result of twice detection, the testing result of twice HCG and diagnostic message is returned " according to this measurement result to user, there is the risk of ectopic pregnancy, please remove hospital admission in time " or " testing result is normal ".If all do not carry out the test of second time urina sanguinis in user after 48 hours 3 hours, then send message " this mensuration is invalid, advises that you check to hospital " to user.Meanwhile, the relevant reference information of ectopic pregnancy can also be sent to user.
By method and system of the present invention, user simply and accurately can carry out test sample by the mobile terminal of oneself, both can do qualitative detection and also can do quantitative detection, eliminates the fatigue and risk of repeatedly going to see a doctor, easily realizes domestic medicine.When needs are repeatedly tested, can be obtained by mobile terminal and remind, avoid missing the best test duration.Doctor also can not have hospital's professional equipment time, the situation beyond hospital realizes portable medical.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; be understood that; the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (44)
1. the test paper based on mobile terminal camera and cloud server reads and an analytical approach, for calculate test paper detect the concentration value of thing to be checked in sample and analytical test result, it is characterized in that comprising the following steps:
A) mobile terminal camera gathers test paper image, analyzes the edge identifying colour developing district, intercepts satisfactory color development area;
B) calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value obtains concentration of specimens ratio;
C) to carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being substituted into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample;
D) search the testing result of coupling according to thing actual concentration value to be checked in sample, and testing result is sent to mobile terminal.
2. the method for claim 1, is characterized in that:
In steps A) before mobile terminal camera gathers test paper image, first camera gathers the identification code of test paper, identification code is sent to cloud server, cloud server call from database detection paper project, batch etc. information return to mobile terminal.
3. the method for claim 1, is characterized in that:
In steps A) before mobile terminal camera gathers test paper image, prompting user, in manual classification, the batch information selecting input test paper of mobile terminal, sends to cloud server.
4. the method for claim 1, is characterized in that:
Steps A) comprise further:
The image of the whole test strips of camera collection;
Color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, find the frame preset, this region selected;
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
5. the method for claim 1, is characterized in that:
Steps A) comprise further:
The image of the whole test strips of camera collection;
The position of screening anchor point, by the position of the anchor point position of color development area, location and level, vertical direction;
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
6. the method for claim 1, is characterized in that:
Steps A) comprise further:
For the common test paper of third party's Non-precondition frame, have the view-finder meeting test paper size on a display screen, prompting user entangles whole test paper or color development area with view-finder, and clicks button of taking pictures.
7. the method as described in claim 1-6, is characterized in that:
Steps A) comprise further:
Mobile terminal carries out standardization to the colour developing district picture intercepted, and photo finishing is become unified pixel size, or is returned by the picture rotation offset on time space of taking pictures or in plane.
8. the method as described in claim 1-6, is characterized in that:
Step B) comprise further:
If color value or the gray-scale value of reference area (C line) are β, color value or the gray-scale value of test section (T line) are α, obtain concentration of specimens compare γ by the relevant ratio computing of α and β.
9. method as claimed in claim 8, is characterized in that:
For nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color value or gray-scale value, as with reference to district's color value or gray-scale value β.
10. the method for claim 1, is characterised in that:
Step C) comprise further:
Corresponding every series-produced test paper product, normal concentration material is used to calibrate this batch products, concentration of specimens ratio is drawn by the ratio computing of test section (T line) and control zone (C line) both color values or gray-scale value, according to the corresponding relation of concentration of specimens ratio and standard substance actual concentration value, produce the typical curve of this batch of test paper, and this typical curve is kept in the database of cloud server;
According to the time dependent change of product that stock keeps sample, upgrade typical curve;
According to the colour developing image of mobile terminal transmission, the concentration of specimens calculated ratio (C, T ratio), carries out fitting operation with the typical curve of this batch, draws the actual concentration value of material to be checked in this inspection.
11. the method for claim 1, is characterized in that:
Step C) further comprising the steps of afterwards:
Judge that this test item is the need of again detecting;
If without the need to again detecting, then the numerical range in the sample actual concentration value of calculating and database being compared, finding out diagnostic result corresponding to this concentration value and corresponding medical advice; Preserve sample values and corresponding testing result, send testing result, corresponding diagnosis and suggestion to mobile terminal;
If need to carry out repeated detection, then preserve this sample actual concentration value, send testing result and detection time next time to mobile terminal; Timer sets the time next time detected, and sends prompting message when timer arrives to mobile terminal, and turns back to step B).
12. 1 kinds of test paper based on mobile terminal camera and cloud server read and analytic system, for calculating the concentration value of sample in test paper and analytical test result, it is characterized in that comprising:
Mobile terminal, for gathering test paper image, analyzing the edge identifying colour developing district, intercepting satisfactory color development area, send to cloud server;
Cloud server, for:
Receive and analyze colour developing district picture, calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio;
To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being substituted into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample;
Search the testing result of coupling according to thing actual concentration value to be checked in sample, and testing result is sent to mobile terminal.
13. systems as claimed in claim 12, is characterized in that:
Test paper for testing comprises immune chromatography test paper or chemical colour reaction test paper, and described test paper comprises identification code, and described identification code comprises Quick Response Code, bar code or other coded systems for identifying, for distinguishing type and the batch information of test paper.
14. systems as described in claim 12-13, is characterized in that:
First mobile terminal gathers the identification code of test paper, identification code is sent to cloud server before gathering test paper image, cloud server call from database detection paper project, batch etc. information temporary storage return to mobile terminal.
15. systems as claimed in claim 12, is characterized in that:
Before mobile terminal gathers test paper image, prompting user, in manual classification, the batch information selecting input test paper of mobile terminal, sends to cloud server.
16. systems as claimed in claim 12, is characterized in that:
Mobile terminal also comprises:
Camera, for gathering the image of whole test strips;
Processing unit, for:
Color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, find the frame preset, this region selected,
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out;
Transmission and reception unit, for sending to cloud server by the color development area image of intercepting.
17. systems as claimed in claim 12, is characterized in that:
Mobile terminal also comprises:
Camera, for gathering the image of whole test strips;
Processing unit, for:
The position of screening anchor point, by the position of the anchor point position of color development area, location and level, vertical direction,
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out;
Transmission and reception unit, for sending to cloud server by the color development area image of intercepting.
18. systems as claimed in claim 12, is characterized in that:
Mobile terminal also comprises:
Touch display screen, for the common test paper for third party's Non-precondition frame, on touch display screen, display meets the view-finder of test paper size, and prompting user entangles whole test paper or color development area with view-finder, and clicks button of taking pictures.
19. systems as described in claim 12-18, is characterized in that:
Described mobile terminal carries out standardization to the colour developing district picture intercepted, and photo finishing is become unified pixel size, or is returned by the picture rotation offset on time space of taking pictures or in plane.
20. systems as described in claim 12-18, is characterized in that:
Cloud server also comprises processing unit, for:
If color value or the gray-scale value of reference area (C line) are β, color value or the gray-scale value of test section (T line) are α, then obtain concentration of specimens by the relevant ratio computing of α and β and compare γ.
21. systems as claimed in claim 20, is characterized in that:
For nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color value or gray-scale value, as with reference to district's color value or gray-scale value β.
22. systems as claimed in claim 12, are characterised in that:
Cloud server also comprises:
Database, for storing the typical curve of each batch of test paper, its standard curve is corresponding every series-produced test paper product, normal concentration material is used to calibrate this batch products, concentration of specimens ratio is drawn by the ratio computing of test section (T line) and control zone (C line) both color values or gray-scale value, according to the corresponding relation of concentration of specimens ratio and standard substance actual concentration value, produce the typical curve of this batch of test paper;
Processing unit, for:
According to the time dependent change of product that stock keeps sample, upgrade typical curve;
According to the colour developing image of mobile terminal transmission, the concentration of specimens ratio calculated (C, T ratio), carries out fitting operation with the typical curve of this batch, draws the actual concentration value of material to be checked in this inspection.
23. 1 kinds of test paper based on mobile terminal camera read and analytic system, for calculating the concentration value of sample in test paper and analytical test result, it is characterized in that comprising:
Mobile terminal, for gathering test paper image, analyzing the edge identifying colour developing district, intercepting satisfactory color development area, send to cloud server;
Cloud server, for:
According to the colour developing district picture received, calculate color value or the gray-scale value of test section (T line) and control zone (C line) in colour developing district picture, the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio;
To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being substituted into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample;
Judge that this test item is the need of again detecting;
If without the need to again detecting, then the numerical range in the actual concentration value of thing to be checked in the sample calculated and database being compared, finding out diagnostic result corresponding to this concentration value and corresponding medical advice; Preserve sample values and cure diagnostic result accordingly, returning testing result, corresponding diagnosis and suggestion to user;
If need to carry out repeated detection, then preserve this sample actual concentration value, send to user and detect sample results and detection time next time; Timer sets the time next time detected, and sends prompting message when timer arrives to the mobile terminal of user, receives the colour developing district picture next time detected.
24. 1 kinds of test paper based on mobile terminal camera and cloud server read and analytic system, for calculating the concentration value of sample in test paper and analytical test result, it is characterized in that comprising:
Mobile terminal, for gathering test paper image, sends to cloud server;
Cloud server, for:
Receive test paper picture, analyze the edge identifying colour developing district, intercept satisfactory color development area;
Calculate color value or the gray-scale value of test section (T line) and control zone (C line) in colour developing district, the ratio computing of both utilizations color value or gray-scale value calculates concentration of specimens ratio;
To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample;
Search the testing result of coupling according to sample actual concentration value, and testing result is sent to mobile terminal.
25. 1 kinds are read and the mobile terminal analyzed for test paper, it is characterized in that comprising:
Camera, for obtaining identification code and the image of test paper;
Touch display screen, for showing the picture material of acquisition, prompting user aims at the tram of test paper;
Processing unit, for analyzing the edge identifying colour developing district, intercepts satisfactory color development area;
Transmission and reception unit, for sending identification code and colour developing district's picture and receiving the testing result that cloud server returns to cloud server.
26. mobile terminals as claimed in claim 25, is characterized in that:
Described processing unit is used for color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, finds the frame preset, this region selected;
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
27. mobile terminals as claimed in claim 25, is characterized in that:
Described processing unit for screening the position of anchor point, by the position of the anchor point position of color development area, location and level, vertical direction;
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
28. mobile terminals as claimed in claim 25, is characterized in that:
Described touch display screen is used for the common test paper for third party's Non-precondition frame, and on touch display screen, display meets the view-finder of test paper size, and prompting user entangles whole test paper or color development area with view-finder, and clicks button of taking pictures.
29. mobile terminals as described in claim 25-28, is characterized in that:
Described processing unit carries out standardization to the colour developing district picture intercepted, and photo finishing is become unified pixel size, or is returned by the picture rotation offset on time space of taking pictures or in plane.
30. 1 kinds are read and the method analyzed for the test paper in mobile terminal, it is characterized in that comprising the following steps:
Camera obtains identification code and the image of test paper;
The picture material that touch display screen display obtains, prompting user aims at the tram of test paper;
Analyze the edge identifying colour developing district, intercept satisfactory color development area;
Send identification code and colour developing district's picture to cloud server and receive the testing result that cloud server returns.
31. methods as claimed in claim 30, is characterized in that:
The described edge analyzing identification colour developing district, the step intercepting satisfactory color development area comprises further:
Color and edge analysis identification, by presetting getting stuck and the aberration of color development area of test paper, find the frame preset, this region selected;
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
32. methods as claimed in claim 30, is characterized in that:
The described edge analyzing identification colour developing district, the step intercepting satisfactory color development area comprises further:
The position of screening anchor point, by the position of the anchor point position of color development area, location and level, vertical direction;
Find out the region that meets and preset proportion requirement in this region as color development area, and intercept out.
33. methods as claimed in claim 30, is characterized in that:
The described edge analyzing identification colour developing district, the step intercepting satisfactory color development area comprises further:
For the common test paper of third party's Non-precondition frame, on touch display screen, display meets the view-finder of test paper size, and prompting user entangles whole test paper or color development area with view-finder, and clicks button of taking pictures.
34. mobile terminals as described in claim 30-33, is characterized in that:
The described edge analyzing identification colour developing district, the step intercepting satisfactory color development area comprises further:
Standardization is carried out to the colour developing district picture intercepted, photo finishing is become unified pixel size, or the picture rotation offset on time space of taking pictures or in plane is returned.
35. 1 kinds are read and the cloud server analyzed for test paper, it is characterized in that comprising:
Sending/receiving module, for receiving test paper color development district picture, and sends testing result to mobile terminal;
Database, for storing the typical curve of each test item different batches, described typical curve is for representing the corresponding relation of measured value and actual concentration value, and diagnostic result corresponding to the actual concentration value storing different test item and medical advice;
Processing unit, for:
Analyze colour developing district picture, calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio;
To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample;
The testing result of coupling is searched according to the actual concentration value of thing to be checked in sample.
36. cloud servers as claimed in claim 35, is characterized in that:
Described processing unit also for:
If color value or the gray-scale value of reference area (C line) are β, color value or the gray-scale value of test section (T line) are α, then obtain concentration of specimens by the relevant ratio computing of α and β and compare γ.
37. cloud servers as claimed in claim 35, is characterized in that:
Described processing unit also for:
For nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color value or gray-scale value, as with reference to district's color value or gray-scale value β.
38. cloud servers as claimed in claim 35, is characterized in that:
The typical curve of described database purchase, it is corresponding every series-produced test paper product, normal concentration material is used to calibrate this batch products, concentration of specimens ratio is drawn by the ratio computing of test section (T line) and control zone (C line) both color values or gray-scale value, according to the corresponding relation of concentration of specimens ratio and standard substance actual concentration value, produce the typical curve of this batch of test paper.
39. cloud servers as claimed in claim 38, is characterized in that:
Described processing unit, according to the time dependent change of product that stock keeps sample, upgrades typical curve.
40. 1 kinds are read and analytical approach for the test paper in cloud server, it is characterized in that comprising:
Receive test paper color development district picture, and send testing result to mobile terminal;
Store the typical curve of each test item different batches, described typical curve is for representing the corresponding relation of measured value and actual concentration value, and diagnostic result corresponding to the actual concentration value storing different test item and medical advice;
Calculate color value or the gray-scale value of test section (T line) in colour developing district picture and control zone (C line), the ratio computing of both utilizations color value or gray-scale value draws concentration of specimens ratio;
To carry out batch calibration to concentration of specimens ratio, typical curve concentration of specimens ratio being brought into this test paper batch carries out fitting operation, calculates the actual concentration value of thing to be checked in this sample;
The testing result of coupling is searched according to the actual concentration value of thing to be checked in sample.
41. methods as claimed in claim 40, is characterized in that:
The step of described calculating sample content comprises further:
If color value or the gray-scale value of reference area (C line) are β, color value or the gray-scale value of test section (T line) are α, then obtain concentration of specimens by the relevant ratio computing of α and β and compare γ.
42. methods as claimed in claim 41, is characterized in that:
The step of described calculating sample content comprises further:
For nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color value or gray-scale value, as with reference to district's color value or gray-scale value β.
43. methods as claimed in claim 40, is characterized in that:
The step of described batch of calibration comprises further: corresponding every series-produced test paper product, normal concentration material is used to calibrate this batch products, concentration of specimens ratio is drawn by the ratio computing of test section (T line) and control zone (C line) both color values or gray-scale value, according to the corresponding relation of concentration of specimens ratio and standard substance actual concentration value, produce the typical curve of this batch of test paper.
44. cloud servers as claimed in claim 43, is characterized in that:
The step of described batch of calibration comprises further: the time dependent change of product kept sample according to stock, upgrades typical curve.
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