CN104829341B - A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer and preparation method thereof - Google Patents
A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to alga fertilizer technical field, more particularly to a kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：10~60% alga oligosaccharides solution, 10~50% liquid molasses, 20~40% complex microorganism bacterium solution, 10~40% big, middle and trace element, the alga oligosaccharides solution is to mix the marine alga after crushing with water, add biological lyases enzymolysis and obtain marine alga enzymolysis liquid, extract in the basic conditions, press filtration and obtain.Present invention simultaneously provides a kind of preparation method of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer.The active ingredient of the invention greatly retained in marine alga, utilization rate of the crop to nutrient can be greatly improved, greatly shorten manufacturing cycle, and necessary organic matter and trace element can be supplemented, add complex microorganism bacterium solution, with disease-resistant, degeneration-resistant, phosphorus decomposing, potassium decomposing, increase the function of soil physics structure, and can effectively prevent and treat all kinds of soil-borne diseases.
Description
Technical field
The invention belongs to alga fertilizer technical field, more particularly to a kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer and its preparation side
Method.
Background technology
Alga fertilizer is natural organic fertilizer, and its fertilizer efficiency depends on nutritional ingredient contained in alga fertilizer, as fertilizer
Marine alga is usually large-scale economic class marine alga.The development experience of alga fertilizer three phases：Rotten marine alga → varec (powder) → sea
Algae extract solution.Core substance in alga fertilizer is pure natural marine algae extract, and primary raw material is selected from natural seaweed, by special life
Change PROCESS FOR TREATMENT, extract the essence substance in marine alga, retain natural activity component, contain substantial amounts of non-nitrogen containing organic matter, Lu Zhi
The incomparable potassium of thing, calcium, magnesium, zinc, iodine etc. more than 40 plants mineral matter element and abundant vitamin, particularly with institute in marine alga
Distinctive algal polysaccharides, alginic acid, highly unsaturated fatty acid and a variety of natural plants growth regulators, with very high biology
Activity, can stimulate the generation of the nonspecific activity factor in plant, adjust Endogenous hormone balance.
Seaweed extracted liquor is the DDGS extracted from large-scale fast-growing brown alga, is the mixture of plurality of active ingredients.Marine alga
Adjusted in liquid containing the growth such as abundant mineral matter, amino acid, vitamins and other nutritious components and the basic element of cell division, auxin, phenols
Material and antibiotics are saved, is applied on cereal crops, water fruits and vegetables, flowers and nursery stock, crop root and master can be promoted
The development of stem, improves yield, and improving quality, enhancing crop resists the ability of poor environment.
Alga fertilizer raw material is selected from natural seaweed, has good compatibility with terrestrial plant, nontoxic to people, animal, to ring
Border is pollution-free, can be used as organic food special fertilizer material.Alga fertilizer can directly make soil or make soil increase organic by plant
Various beneficial microbes in matter, activation soil, these biologies can play catalyst in plant-microbe metabolite circulation
Effect, making the biopotency of soil increases.The metabolin of plant and edaphon can provide more nutrients for plant.Sea
Algae fertilizer is a kind of natural biological preparation, can harmoniously be worked with plant-soil ecosystem.The native compound that it contains
If Sodium Alginate is natural soil conditioner, the formation of soil granular structure can be promoted, improve soil internal void space, coordinated
Solid, liquid, gas proportions in soil, recover due to soil over-burden and chemical contamination and lose lac balance, increase
Plus geobiont vigor, promote the release of available nutrient, be conducive to root growth, improve the resistance of crop.
According to another statistics, the only large-scale economical alga of China just has kind more than 100, wherein it is no lack of the species for having high fertilizer efficiency, resource ten
Divide abundant, development and application alga fertilizer has a extensive future.
At present, with the development of agricultural, alga fertilizer is used in combination with biological bacteria as a new research direction, microorganism
Bacterium and organic substance combine, and improve soil texture and soil micro-ecosystem system, and rehabilitating soil plays global advantage, to build
Found benign farmland's ecocycle system and crop nutrition combined system.But, add at present in the alga fertilizer of biological bacteria, alga fertilizer
Interior bioactive substance destruction is than more serious, and addition strain is single, is improving utilization rate of fertilizer, is improving soil texture,
Variation of Integrated Soil Fertility is improved, promotes effective microorganisms body to be formed, effect is undesirable in terms of enhancing disease-resistant crops ability.
Influence of the production method of marine alga fertilizer to product bioactive substance and nutrient composition content is very big, current marine alga
The production of fertilizer is mainly using chemical hydrolysis, physics extraction method, enzymatic isolation method, biological fermentation process etc., using the strong of chemical hydrolysis
Alkali hot environment can break the activity of meeting marine alga endogenous substance, and physics extraction method is that the technique cooled down using high-temperature low-pressure reaches marine alga
The broken purpose of cell membrane, better than chemical hydrolysis, but working condition is harsh, and production cost is higher, and industrialization conversion is existed
Bigger difficulty, enzymatic isolation method and biological fermentation process are that the macromolecular substances for constituting marine alga are degraded by enzymolysis or fermentation process
Into small molecule, water miscible material, the bioactive substance and nutriment in marine alga are remained to greatest extent, and can be by
Plant absorption is utilized, but also there are many defects, and one is that fermentative microorganism strain is single, and the enzyme system of generation is single, it is difficult to
The macromolecular substances of real degraded marine alga, influence the action effect of marine alga fertilizer, two be that biological fermentation process prepares marine alga fertilizer also
In the presence of using microbial fermentation strain to soil without any beneficial effect, simple adds production cost.
The content of the invention
It is an object of the invention to provide a kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, it can greatly retain the biology in marine alga
Active material and nutriment, add composite bacteria, easy to use, effectively improve utilization rate of fertilizer, improve soil texture, improve
Variation of Integrated Soil Fertility, promotes effective microorganisms body to be formed, and strengthens disease-resistant crops ability.
The present invention is achieved in that a kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：
10~60% alga oligosaccharides solution, 10~50% liquid molasses, 20~40% complex microorganism bacterium solution, 10~40%
Greatly, middle and trace element, the alga oligosaccharides solution is to mix the marine alga after crushing with water, adds biological lyases and digests
To marine alga enzymolysis liquid, extract in the basic conditions, press filtration and obtain.
As an improvement, the marine alga after the crushing presses 1 with water：The weight of (4~7) accounts for the sea than mixing, addition
Algae and the weight of water mixed liquid 0.2~1% than biological lyases, temperature be 35~75 DEG C, pH value be enzymolysis under the conditions of 3~5
Obtain marine alga enzymolysis liquid within 12~24 hours, the pH value for then adjusting marine alga enzymolysis liquid is obtained to 8.0~8.5, then by press filtration
The weight content of alga oligosaccharides is more than or equal to 3.5% in the alga oligosaccharides solution, the alga oligosaccharides solution.
As a further improvement, potassium hydroxide solution is added in the marine alga enzymolysis liquid adjusts pH value to 8.0~8.5.
As a further improvement, the biological lyases includes Alginate Lyases and cellulase.
As a further improvement, the Alginate Lyases are endo-type mannuronic acid enzyme.
As an improvement, the complex microorganism bacterium solution is in bacillus bacterium solution, EM bacterium solutions, actinomyces bacterium solution
One kind, or several be composited by therein.
As an improvement, the big, middle and trace element includes urea, potassium dihydrogen phosphate, potassium nitrate, chelating calcium, chelating
Two or more in magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax.
, can be great another object of the present invention is to provide a kind of preparation method of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer
Retain the active ingredient in natural seaweed, greatly improve utilization rate of the crop to nutrient, and the organic of needs can be supplemented
Matter, trace element, and the cycle for preparing alga oligosaccharides can be greatly shortened.
A kind of preparation method of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, the preparation method comprises the following steps：
(1) preparation of alga oligosaccharides solution：By marine alga dries pulverizing, the sea-tangle after crushing is added to the water to obtain mixed liquor,
The part by weight of sea-tangle and water is 1：(4~7), the life for accounting for mixed liquor weight 0.2~1% is added into the mixed liquor of sea-tangle and water
Thing lyases, under the conditions of temperature is 35~75 DEG C, pH value is 3~5, digests 12~24 hours, obtains marine alga enzymolysis liquid, Xiang Hai
Potassium hydroxide solution is added in algae enzymolysis liquid makes pH value adjust to 8~8.5, is filtered by filtering equipment, filters residue, that is, obtain
Alga oligosaccharides solution；
(2) culture of complex microorganism bacterium solution：By the strain in complex microorganism bacterium solution, fermented and cultured obtains each bacterium respectively
The single bacterium solution planted, is then compounded single bacterium solution, and complex microorganism bacterium solution is made；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at
70 DEG C of progress chelatropic reactions, add obtained complex microorganism bacterium solution in step (2), stir after cooling.
As an improvement, add lactic acid bacteria in the enzymolysis process of the step (1).
As an improvement, the single bacterium solution equal proportion compounding of each strain in the step (2).
Due to using above-mentioned technical proposal, the beneficial effects of the invention are as follows：
The alga oligosaccharides Tiny ecosystem drip irrigation fertilizer that the present invention is provided, organic materials is mixed with complex microorganism bacterium solution, and adds
Increase, middle and trace element and organic fertilizer is made, wherein, alga oligosaccharides solution is prepared by enzymolysis, is avoided in the past using pure
Chemical method is extracted by high temperature and high pressure method, keeps the activity of bioactive substance in marine alga, greatly retains effective in marine alga
Composition, can greatly improve utilization rate of the crop to nutrient, greatly shorten manufacturing cycle, and can supplement necessary organic matter
And trace element, complex microorganism bacterium solution includes multiple beneficial mushroom, and microorganism carries out vital movement in soil, not only can be with
The n-p-k element supply absorption level of plant is improved, so as to improve plant nutrient situation and increase outside yield, can also be produced
All kinds of auximones stimulate plant growths, some pathogenic microorganisms of antagonism and suppress pest and disease damage, with disease-resistant, degeneration-resistant, phosphorus decomposing,
Potassium decomposing, increases the function of soil physics structure, and can effectively prevent and treat all kinds of soil-borne diseases.
Alginate Lyases and cellulase are used during alga oligosaccharides in-solution digestion, cellulase can digest sea
Alginate in the cellulose tissue formation carbohydrate of algae, Alginate Lyases degraded marine alga, and Alginate Lyases
Substratspezifitaet and alga oligosaccharides type be adapted, belong to endo-type mannuronic acid enzyme, be by marine organisms
Extract, with selectivity, compared with general enzyme, it digests speed and recovery rate is greatly improved.Use potassium hydroxide solution
Adjust the pH value of marine alga enzymolysis liquid so that the main component of alga oligosaccharides is potassium alginate, greatly increases potassium element in fertilizer and contains
Amount, and in alga oligosaccharides solution potassium alginate content more than 3.5%, preparation technology is simple, and can guarantee that in fertilizer
The high content of alga oligosaccharides.
Specific hydrolysis temperature is control between 35-75 degree, pH when less than 5 from degradation speed substantially accelerate,
Alginic acid can be dehydrated precipitation during less than 3, therefore pH controls, between 3-5, enzymolysis speed is fast, while greatly retaining having in marine alga
Composition is imitated, and lactic acid bacteria is added into the mixed liquor of marine alga and water in enzymolysis process, the meeting in enzymolysis process due to marine alga
Spontaneous fermentation is carried out, during fermentative degradation, organic substance can produce some corrupt substances, zymotic fluid can be made to become stench,
Lactic acid bacteria can produce lactein, can contain the generation of this phenomenon, and lactic acid bacteria can reduce the pH in enzymolysis process
Value is 8.0-8.5 so as to promote to keep the pH value of marine alga enzymolysis liquid in the enzymolysis of marine alga, pressure-filtering process, it is to avoid strong basicity environment is broken
The activity of bioactive substance in bad marine alga enzymolysis liquid, takes into account the dissolubility requirement of potassium alginate again.
Liquid molasses are that the nutrients such as honey, nitrogen, phosphorus, calcium, sodium, trace element, vitamin are added in molasses culture medium
Fermented bacterium is added in the culture medium of composition, closing fermentation is carried out under the aseptic condition in stainless steel fermentation tank, fermentation is extracted
Culture medium afterwards forms molasses after low temperature dewatering, evaporation and concentration, can be with middle trace element containing abundant organic principle
Liquid molasses are mixed and then added big, middle and trace element by generation chelatropic reaction, the present invention with alga oligosaccharides solution first, wherein
Middle trace element including Chelating state, and carry out chelatropic reaction so that in trace element chelated effect more preferably, function nutrition group
More stablize, be not susceptible to be lost in soil, effectively occur antagonism, nutrient absorption profit between the middle trace element of prevention addition
More than 30% can be improved with rate.
Complex microorganism bacterium solution is one kind in bacillus bacterium solution, EM bacterium solutions, actinomyces bacterium solution, or by therein
Several to be composited, huge microorganism species enter after soil, can improve the permeability of soil rapidly, probiotics is in soil
Metabolin increase natural growth hormone to crops, with disease-resistant, degeneration-resistant, phosphorus decomposing, potassium decomposing, increase soil physics structure work(
Can, and all kinds of soil-borne diseases can be effectively prevented and treated, each strain obtains the single bacterium solution of each strain after individually being fermented respectively,
The mixing progress of single bacterium solution is fermented again and obtains complex micro organism fungicide, composite microbiological fertilizer prepared by this method can be protected effectively
The concertedness held between bacterial activity, strain is strong, and fertilizer efficiency is high.
The present invention not only uses cooperatively N P and K and trace element, and by mineral matter element, microbial bacteria and organic
Material combines, and plays global advantage, sets up benign farmland's ecocycle system and crop nutrition combined system, make full use of
Soil capabilities, reaching makes crop yield increase income optimum efficiency.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment one
A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：20% alga oligosaccharides solution,
20% liquid molasses, 40% complex microorganism bacterium solution, 20% big, middle and trace element.
Above-mentioned alga oligosaccharides solution is by 1 by the marine alga after crushing and water：4 weight accounts for marine alga and water than mixing, addition
The biological lyases of mixed liquor weight 0.5%, adds the lactic acid bacteria for accounting for marine alga and water mixed liquid weight 1%, temperature be 60 DEG C,
PH value be 3~5 under the conditions of, enzymolysis obtain marine alga enzymolysis liquid within 12 hours, then in marine alga enzymolysis liquid add potassium hydroxide solution adjust
PH value is saved to 8.0~8.5, then carries out press filtration by filter press and obtains alga oligosaccharides solution, marine alga is few in alga oligosaccharides solution
The main component of sugar is potassium alginate, and its weight content is 3.9%.
Wherein, biological lyases includes Alginate Lyases and cellulase, and Alginate Lyases are that endo-type is sweet
Reveal uronic acid enzyme；Complex microorganism bacterium solution is composited by bacillus bacterium solution and EM bacterium solutions；The big, middle and trace element
Including urea, potassium nitrate, chelated iron, chelated zinc, boric acid.
The preparation method of above-mentioned alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, comprises the following steps：
(1) preparation of alga oligosaccharides solution：By sea-tangle dries pulverizing, the sea-tangle after crushing is added to the water to obtain mixed liquor,
The part by weight of sea-tangle and water is 1：4, the biological cracking for accounting for mixed liquor weight 0.5% is added into the mixed liquor of sea-tangle and water
Enzyme, under the conditions of temperature is 60 DEG C, pH value is 3~5, is digested 12 hours, and addition accounts for sea-tangle in enzymolysis process and water is mixed
Close the lactic acid bacteria of liquid weight 1%, obtain marine alga enzymolysis liquid, added into marine alga enzymolysis liquid potassium hydroxide solution make pH value adjust to
8.0~8.5, filtered by filtering equipment, filter residue, that is, obtain alga oligosaccharides solution；
(2) culture of complex microorganism bacterium solution：It it is 35 DEG C in temperature, under confined conditions, by aerobic Bacillus strain
Inoculation, activation culture 10~12 hours in activation medium, then train cultured strain in production fermentation respectively respectively
Fermentation is expanded numerous 24~72 hours in foster base, is constantly shaken or is stirred during activation culture and fermentation expansion are numerous, obtains each seed bud
Spore bacillus bacterium solution；EM strains are connected in the culture medium of high-temperature sterilization, anaerobic fermentation 24~48 hours obtains EM bacterium solutions, then
By bacillus bacterium solution and EM bacterium solutions according to weight ratio 1:1 ratio is mixed to get complex microorganism bacterium solution；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at
70 DEG C of progress chelatropic reactions, chelatropic reaction 45 minutes, obtained complex microorganism bacterium solution in cooling addition step (2), is stirred afterwards
Mix uniform.
Embodiment two
A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：10% alga oligosaccharides solution,
30% liquid molasses, 30% complex microorganism bacterium solution, 30% big, middle and trace element.
Above-mentioned alga oligosaccharides solution is by 1 by the sargassum after crushing and water：4 weight accounts for sargassum than mixing, addition
With the weight of water mixed liquid 0.2% than biological lyases, temperature be 35 DEG C, pH value be under the conditions of 3~5, enzymolysis 16 hours
To marine alga enzymolysis liquid, and the lactic acid bacteria for accounting for marine alga and water mixed liquid weight 0.5% is added in enzymolysis process, then marine alga enzyme
Solve and potassium hydroxide solution is added in liquid adjust pH value to 8.0~8.5, then carry out press filtration by filter press and to obtain alga oligosaccharides molten
The main component of alga oligosaccharides is potassium alginate in liquid, alga oligosaccharides solution, and its weight content is 3.8%.
Wherein, biological lyases includes Alginate Lyases and cellulase, and Alginate Lyases are that endo-type is sweet
Reveal uronic acid enzyme；Complex microorganism bacterium solution is composited by bacillus bacterium solution and actinomyces bacterium solution；It is described big or middle, micro
Element includes urea, potassium dihydrogen phosphate, potassium nitrate, chelated iron, chelated zinc, Chelated Manganese, ammonium molybdate, boric acid.
The preparation method of above-mentioned alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, comprises the following steps：
(1) preparation of alga oligosaccharides solution：By sargassum dries pulverizing, the sargassum after crushing, which is added to the water, to be mixed
The part by weight of liquid, sargassum and water is 1：4, the life for accounting for mixed liquor weight 0.2% is added into the mixed liquor of sargassum and water
Thing lyases, under the conditions of temperature is 35 DEG C, pH value is 3~5, is digested 16 hours, and addition accounts for marine alga in enzymolysis process
With the lactic acid bacteria of water mixed liquid weight 0.5%, marine alga enzymolysis liquid is obtained, potassium hydroxide solution is added into marine alga enzymolysis liquid makes pH
Value regulation is filtered by filtering equipment to 8.0~8.5, filters residue, that is, obtain alga oligosaccharides solution；
(2) culture of complex microorganism bacterium solution：It it is 35 DEG C in temperature, under confined conditions, by aerobic Bacillus strain
Inoculation, activation culture 10~12 hours in activation medium, then train cultured strain in production fermentation respectively respectively
Fermentation is expanded numerous 24~72 hours in foster base, is constantly shaken or is stirred during activation culture and fermentation expansion are numerous, obtains gemma bar
Bacterium bacterium solution；Actinomyces strain is inoculated into cornstarch and accounts for 2.5wt%, soybean cake powder accounts for 3.5~4wt%, and corn steep liquor is accounted for
1.5wt%, thiamines accounts for 1wt%, and soybean oil is accounted in 1% fluid nutrient medium, is 28 DEG C in temperature, between pH value 6.0~7.5,
Actinomyces bacterium solution is made in 24 hours in culture, then by bacillus bacterium solution and actinomyces bacterium solution according to weight ratio 1:1
Ratio is mixed to get complex microorganism bacterium solution；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at
70 DEG C of progress chelatropic reactions, chelatropic reaction is cooled down after 45 minutes, obtained complex microorganism bacterium solution, stirring in addition step (2)
It is uniform.
Embodiment three
A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：60% alga oligosaccharides solution,
10% liquid molasses, 20% complex microorganism bacterium solution, 10% big, middle and trace element.
Above-mentioned alga oligosaccharides solution is by 1 by the sea-tangle after crushing and water：7 weight accounts for sea-tangle and water than mixing, addition
The weight of mixed liquor 1% than biological lyases, temperature be 75 DEG C, pH value be that under the conditions of 3~5, enzymolysis obtains marine alga in 24 hours
Enzymolysis liquid, and the lactic acid bacteria for accounting for marine alga and water mixed liquid weight 0.8% is added in enzymolysis process, then in marine alga enzymolysis liquid
Add potassium hydroxide solution and adjust pH value to 8.0~8.5, then carry out press filtration by filter press and obtain alga oligosaccharides solution, sea
The main component of alga oligosaccharides is potassium alginate in algae oligosaccharide solution, and its percentage by weight is 3.7%.
Wherein, biological lyases includes Alginate Lyases and cellulase, and Alginate Lyases are that endo-type is sweet
Reveal uronic acid enzyme；Complex microorganism bacterium solution is composited by EM bacterium solutions and actinomyces bacterium solution；Big, the middle and trace element bag
Include urea, potassium dihydrogen phosphate, potassium nitrate, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boron
Acid, two or more in borax.
The preparation method of above-mentioned alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, comprises the following steps：
(1) preparation of alga oligosaccharides solution：By sea-tangle dries pulverizing, the sea-tangle after crushing is added to the water to obtain mixed liquor,
The part by weight of sea-tangle and water is 1：7, the biological lyases for accounting for mixed liquor weight 1% is added into the mixed liquor of sea-tangle and water,
Under the conditions of temperature is 75 DEG C, pH value is 3~5, is digested 24 hours, and addition accounts for sea-tangle and water mixed liquid in enzymolysis process
The lactic acid bacteria of weight 0.8%, obtains marine alga enzymolysis liquid, into marine alga enzymolysis liquid add potassium hydroxide solution make pH value adjust to
8.0~8.5, filtered by filtering equipment, filter residue, that is, obtain alga oligosaccharides solution；
(2) culture of complex microorganism bacterium solution：EM strains are connected in the culture medium of high-temperature sterilization, anaerobic fermentation 24~48
Hour, obtain EM bacterium solutions；Actinomyces strain is inoculated into cornstarch and accounts for 2.5wt%, soybean cake powder accounts for 3.5~4wt%, it is beautiful
Rice ＆ peanut milk accounts for 1.5wt%, and thiamines accounts for 1wt%, and soybean oil is accounted in 1% fluid nutrient medium, is 28 DEG C, pH value 6.0~7.5 in temperature
Between, actinomyces bacterium solution is made in 24 hours in culture, then by EM bacterium solutions and actinomyces bacterium solution according to weight ratio 1:1 ratio
Example is mixed to get complex microorganism bacterium solution；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at
70 DEG C of progress chelatropic reactions, chelatropic reaction is cooled down after 1 hour, and obtained complex microorganism bacterium solution in addition step (2), stirring is equal
It is even.
Example IV
A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：20% alga oligosaccharides solution,
50% liquid molasses, 20% complex microorganism bacterium solution, 10% big, middle and trace element.
Above-mentioned alga oligosaccharides solution is by 1 by the sargassum after crushing and water：6 weight accounts for sargassum than mixing, addition
With the weight of water mixed liquid 0.8% than biological lyases, temperature be 50 DEG C, pH value be under the conditions of 3~5, enzymolysis 18 hours
To marine alga enzymolysis liquid, and the lactic acid bacteria for accounting for marine alga and water mixed liquid weight 1.2% is added in enzymolysis process, then marine alga enzyme
Solve and potassium hydroxide solution is added in liquid adjust pH value to 8.0~8.5, then carry out press filtration by filter press and to obtain alga oligosaccharides molten
The main component of alga oligosaccharides is potassium alginate in liquid, alga oligosaccharides solution, and its percentage by weight is 3.6%.
Wherein, biological lyases includes Alginate Lyases and cellulase, and Alginate Lyases are that endo-type is sweet
Reveal uronic acid enzyme；Complex microorganism bacterium solution is composited by bacillus bacterium solution, EM bacterium solutions, actinomyces bacterium solution；It is described it is big,
Middle and trace element includes urea, potassium dihydrogen phosphate, potassium nitrate, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, chelating
Manganese, ammonium molybdate, boric acid, borax.
The preparation method of above-mentioned alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, comprises the following steps：
(1) preparation of alga oligosaccharides solution：By sargassum dries pulverizing, the sargassum after crushing, which is added to the water, to be mixed
The part by weight of liquid, sargassum and water is 1：6, the life for accounting for mixed liquor weight 0.8% is added into the mixed liquor of sargassum and water
Thing lyases, under the conditions of temperature is 50 DEG C, pH value is 3~5, is digested 18 hours, and addition accounts for marine alga in enzymolysis process
With the lactic acid bacteria of water mixed liquid weight 1.2%, marine alga enzymolysis liquid is obtained, potassium hydroxide solution is added into marine alga enzymolysis liquid makes pH
Value regulation is filtered by filtering equipment to 8.0~8.5, filters residue, that is, obtain alga oligosaccharides solution；
(2) culture of complex microorganism bacterium solution：It it is 35 DEG C in temperature, under confined conditions, by aerobic Bacillus strain
Inoculation, activation culture 10~12 hours in activation medium, then train cultured strain in production fermentation respectively respectively
Fermentation is expanded numerous 24~72 hours in foster base, is constantly shaken or is stirred during activation culture and fermentation expansion are numerous, obtains gemma bar
Bacterium bacterium solution；EM strains are connected in the culture medium of high-temperature sterilization, anaerobic fermentation 24~48 hours obtains EM bacterium solutions；By actinomyces
Category strain is inoculated into cornstarch and accounts for 2.5wt%, and soybean cake powder accounts for 3.5~4wt%, and corn steep liquor accounts for 1.5wt%, and thiamines is accounted for
1wt%, soybean oil is accounted in 1wt% fluid nutrient medium, is 28 DEG C or so in temperature, pH is 6.0~7.5, and culture 24 hours is
Actinomyces bacterium solution is made, respectively by bacillus bacterium solution, EM bacterium solutions and actinomyces bacterium solution according to weight ratio 1:1:1 ratio
It is mixed to get complex microorganism bacterium solution；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at
70 DEG C of progress chelatropic reactions, chelatropic reaction is cooled down after 1 hour, and obtained complex microorganism bacterium solution in addition step (2), stirring is equal
It is even.
Embodiment five
A kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, including following components in percentage by weight：20% alga oligosaccharides solution,
20% liquid molasses, 20% complex microorganism bacterium solution, 40% big, middle and trace element.
Above-mentioned alga oligosaccharides solution is by 1 by the sea-tangle after crushing and water：5 weight accounts for sea-tangle and water than mixing, addition
The weight of mixed liquor 0.4% than biological lyases, temperature be 50 DEG C, pH value be that under the conditions of 3~5, enzymolysis obtains sea in 16 hours
Algae enzymolysis liquid, and the lactic acid bacteria for accounting for marine alga and water mixed liquid weight 1.5% is added in enzymolysis process, then marine alga enzymolysis liquid
Middle addition potassium hydroxide solution adjusts pH value to 8.0~8.5, then carries out press filtration by filter press and obtain alga oligosaccharides solution,
The main component of alga oligosaccharides is potassium alginate in alga oligosaccharides solution, and its weight content is 3.7%.
Wherein, biological lyases includes Alginate Lyases and cellulase, and Alginate Lyases are that endo-type is sweet
Reveal uronic acid enzyme；Complex microorganism bacterium solution is EM bacterium solutions；The big, middle and trace element includes urea, potassium dihydrogen phosphate, nitric acid
Potassium, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boric acid, borax.
The preparation method of above-mentioned alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, comprises the following steps：
(1) preparation of alga oligosaccharides solution：By marine alga dries pulverizing, the sea-tangle after crushing is added to the water to obtain mixed liquor,
The part by weight of sea-tangle and water is 1：6, the biological cracking for accounting for mixed liquor weight 0.4% is added into the mixed liquor of sea-tangle and water
Enzyme, under the conditions of temperature is 50 DEG C, pH value is 3~5, is digested 16 hours, and addition accounts for marine alga in enzymolysis process and water is mixed
The lactic acid bacteria of liquid weight 1.5% is closed, marine alga enzymolysis liquid is obtained, potassium hydroxide solution is added into marine alga enzymolysis liquid adjusts pH value
To 8.0~8.5, filtered by filtering equipment, filter residue, that is, obtain alga oligosaccharides solution；
(2) culture of complex microorganism bacterium solution：It is 35 DEG C in temperature, under confined conditions, EM strains is connected to high-temperature sterilization
Culture medium in, anaerobic fermentation 24~48 hours obtains EM bacterium solutions；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at
70 DEG C of progress chelatropic reactions, chelatropic reaction is cooled down after 80 minutes, obtained complex microorganism bacterium solution, stirring in addition step (2)
It is uniform.
By alga oligosaccharides Tiny ecosystem drip irrigation fertilizer made from embodiment one, every mu of 1~10KG of administration dilutes 300~500 times of drops
Fill or punching is applied, tested in Experimental Base, compared with peasant's conventional fertilizer application, comparing result such as following table：
Pass through the administration to crops such as cucumber, eggplant, apple, citrus and grapes, it can be seen that with peasant's conventional fertilizer application phase
Than that can improve crop yield more than 15%, while enhancing resistance against diseases, improve product quality, it is seen then that the present invention is provided
Alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, greatly improve N P and K and middle micro- utilization rate in soil, effectively reduction plantation
Cost.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (6)
1. a kind of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer, it is characterised in that consist of the following components in percentage by weight：10~60%
Alga oligosaccharides solution, 10~50% liquid molasses, 20~40% complex microorganism bacterium solution, 10~40% it is big or middle, micro
Element；
The alga oligosaccharides solution is by 1 by the marine alga after crushing and water：The weight of (4~7) accounts for the marine alga than mixing, addition
With the weight of water mixed liquid 0.2~1% than biological lyases, temperature be 35~75 DEG C, pH value be enzymolysis 12 under the conditions of 3~5
Obtain marine alga enzymolysis liquid within~24 hours, the pH value for then adjusting marine alga enzymolysis liquid obtains institute to 8.0~8.5, then by press filtration
The weight content for stating alga oligosaccharides in alga oligosaccharides solution, the alga oligosaccharides solution is more than or equal to 3.5%；
Potassium hydroxide solution is added in the marine alga enzymolysis liquid and adjusts pH value to 8.0~8.5；
Fermented bacterium is added in the culture medium that the liquid molasses are made up of honey, nitrogen, phosphorus, calcium, sodium, trace element, vitamin,
Closing fermentation is carried out under aseptic condition in stainless steel fermentation tank, the culture medium after fermentation is extracted by low temperature dewatering, evaporation
Formed after concentration；
The biological lyases is endo-type mannuronic acid enzyme and cellulase.
2. alga oligosaccharides Tiny ecosystem drip irrigation fertilizer according to claim 1, it is characterised in that the complex microorganism bacterium solution is
One kind in bacillus bacterium solution, EM bacterium solutions, actinomyces bacterium solution, or several be composited by therein.
3. alga oligosaccharides Tiny ecosystem drip irrigation fertilizer according to claim 1, it is characterised in that big, the middle and trace element bag
Include urea, potassium dihydrogen phosphate, potassium nitrate, chelating calcium, chelated magnesium, chelated iron, chelated zinc, chelated copper, Chelated Manganese, ammonium molybdate, boron
Acid, two or more in borax.
4. a kind of preparation method of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer as claimed in claim 1, it is characterised in that including following
Step：
(1) preparation of alga oligosaccharides solution：By marine alga dries pulverizing, the marine alga after crushing is added to the water to obtain mixed liquor, marine alga
Part by weight with water is 1：(4~7), add into the mixed liquor of marine alga and water and account for the biology of mixed liquor weight 0.2~1% and split
Enzyme is solved, under the conditions of temperature is 35~75 DEG C, pH value is 3~5, digests 12~24 hours, marine alga enzymolysis liquid is obtained, to marine alga enzyme
Adding potassium hydroxide solution in solution liquid makes pH value adjust to 8~8.5, is filtered by filtering equipment, filters residue, that is, obtain marine alga
Oligosaccharide solution；
(2) culture of complex microorganism bacterium solution：By the strain in complex microorganism bacterium solution, fermented and cultured obtains each strain respectively
Single bacterium solution, is then compounded single bacterium solution, and complex microorganism bacterium solution is made；
(3) into step (1), obtained alga oligosaccharides solution adds liquid molasses and big, middle and trace element, maintains the temperature at 70 DEG C
Chelatropic reaction is carried out, obtained complex microorganism bacterium solution in step (2) is added after cooling, is stirred.
5. the preparation method of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer according to claim 4, it is characterised in that the step
(1) lactic acid bacteria is also added in enzymolysis process.
6. the preparation method of alga oligosaccharides Tiny ecosystem drip irrigation fertilizer according to claim 4, it is characterised in that the step
(2) the single bacterium solution equal proportion compounding of each strain in.
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