CN104817376A - Bio-organic fertilizer prepared from lysine waste mother liquor - Google Patents
Bio-organic fertilizer prepared from lysine waste mother liquor Download PDFInfo
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- CN104817376A CN104817376A CN201510168335.9A CN201510168335A CN104817376A CN 104817376 A CN104817376 A CN 104817376A CN 201510168335 A CN201510168335 A CN 201510168335A CN 104817376 A CN104817376 A CN 104817376A
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Abstract
The invention belongs to the field of fermentation wastewater disposal in the bio-fermentation industry and discloses a bio-organic fertilizer prepared from lysine waste mother liquor. According to the bio-organic fertilizer, fermentation waste mother liquor and bio-organic matters are effectively utilized, and ammonification and granulation technologies are adopted. During the overall process of the bio-organic fertilizer, no waste is generated. The bio-organic fertilizer is environmentally friendly and pollution-free. The fertilizer is prepared while high-purity protein and feeds are also prepared.
Description
Technical field
The invention belongs to biological fermentation industry Wastewater treating field, be specifically related to the biological organic fertilizer utilizing Methionin waste mother liquor to prepare.
Background technology
Methionin is one of essential amino acid, can promote human development, strengthen immunologic function, and be improved the effect of central nervous tissue function.Methionin is alkaline indispensable amino acid.Because the lysine content in cereal foods is very low, and be easily destroyed in the course of processing and lack, therefore being called the first limiting amino acids.Methionin is the seed amino acid that human body needs, a kind of indispensable nutritive substance.People know, protein is the major ingredient forming human body cell, through digesting first breaks down into amino acids after protein in food enters human body, then human body utilizes again the human body protein that these amino acid resynthesis are new, as immune antibody, digestive ferment, plasma proteins, tethelin etc. be all synthesis after human body protein.In each seed amino acid of synthetic protein, 1B is most important one, has lacked it, and other amino acid is just restricted or is not used, and scientist claims it to be human body first indispensable amino acid.Scientist also finds, 1B is that to press down most important in long element (Somatotation, ss) be also the most required composition to the important substance controlling growth in humans, all plays an important role to the nervus centralis of people and peripheral nervous system.Human body self can not synthesize 1B, must draw Methionin from food is the key substance helping other nutritive substance fully to be absorbed by human body and utilize, human body only supplements absorption and the utilization that enough 1Bs could improve food proteins, reach balanced nutritious, enhancing development.At present, the main method of producing Methionin comprises two-step fermenting, direct fermentation, enzyme process etc.But the cost of current fermention medium is higher, the amino acid whose enterprise of a lot of production faces loss, and how reducing fermentation costs is the problem that enterprise needs to solve.
At present, fermentation byproduct tropina is by after flocculation sediment Plate Filtration, and directly sell as cheap feedstuff protein, added value of product is lower; Waste water is directly discharged by simple process; But various waste material does not process by aforesaid method respectively, does not utilize added value to greatest extent.How effectively utilizing fermented abandoned mother liquor, improve the added value of industry to greatest extent, is the direction that enterprise is pursued always.
The development of China's fertilizer and application history, be main phase from farm manure to use inorganic fertilizer, because farm manure source of pollution are many, traffic capacity is large, operationally wastes time and energy and effect is not obvious especially; And inorganic fertilizer composition is single, utilization ratio is lower, and often causes soil compaction and adjoint soil erosion polluted source, influence ecological environment.At present, the inorganic composite fertilizer etc. of market many employings Nitrogen-and Phosphorus-containing potassium, the fertilizer efficiency of this composite fertilizer is comprehensive, but it is lasting also to there is fertilizer efficiency, the defects such as easy loss; The more important thing is, the price of current composite fertilizer is higher, brings larger burden to peasant, how to reduce fertilizer cost, and improving farmers' income is the agricultural modernization technical issues that need to address.
Summary of the invention
In order to overcome the defect of prior art, the invention provides the biological organic fertilizer utilizing Methionin waste mother liquor to prepare, this biological organic fertilizer effectively make use of waste mother liquor composition, and whole process produces without waste, environment friendly and pollution-free; While preparing fertilizer, high purity protein and feed are also prepared.
For achieving the above object, the present invention is by the following technical solutions:
Utilize biological organic fertilizer prepared by Methionin waste mother liquor, the preparation method of described biological organic fertilizer comprises the steps:
Step 1) lysine fermentation liquor is the strainer of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrated solution and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation Threonine crystal and waste liquid; Merge above-mentioned trapped substance, concentrated solution and waste liquid, obtain waste mother liquor;
Step 2) waste mother liquor is heated, make temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 12min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying ovens to dry to constant weight, uviolizing 5min subsequently; Then thalline is added in stirred reactor, and add the hydrochloric acid soln of the 1M being equivalent to thalline double weight, 100 turns/min stirring reaction 15min, cell walls is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant liquor, be condensed into paste, finally by spray granulating fluidised bed drying, obtain albumen;
Step 3) is by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell walls merges, and natural subsidence solid-liquid separation, obtains supernatant liquor and throw out; Throw out, sorghum flour, rice chaff, egg shell powder and the wheat bran mass ratio according to 5:2:1:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment water ratio to 50%, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of air seasonings, pulverize and obtain ox feed;
Step 4) adds potassium humate, diatomite, zeolite powder, dregs of beans and the peat composed of rotten mosses in step 3) gained supernatant liquor, stirs, and leaves standstill 12 hours, then adds ammoniacal liquor, stir, and to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, monoammonium phosphate, poly aspartic acid, zinc sulfate are mixed according to the mass ratio of 200:10:1:1, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging.
Preferably,
In described step 1), microfiltration membrane is inorganic ceramic film, and molecular weight cut-off is 3000Da, and micro-filtration temperature is 36 DEG C; Ultra-filtration membrane molecular weight cut-off is 300Da, and ultrafiltrate temperature is 36 DEG C.
Described ultraviolet intensity is 2000uW/cm
2.
The enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g.
Described sorghum flour and egg shell powder are pulverized obtained by Chinese sorghum and Ovum crusta Gallus domesticus respectively.
In described step 4), the mass ratio of step 3) gained supernatant liquor, potassium humate, diatomite, zeolite powder, dregs of beans, the peat composed of rotten mosses and ammoniacal liquor is 100:30:10:8:6:6:5.
Beneficial effect of the present invention mainly comprises:
Because Methionin waste mother liquor contains a large amount of fermentation thalli, albumen, sugar and fiber substance, the present invention, while production Methionin, make use of waste mother liquor, eliminate mother liquid disposal process, reduce production cost, achieve and turn waste into wealth, increased substantially the utilization ratio of resource; The present invention has prepared high purity protein, feed and biological organic fertilizer simultaneously, and effectively make use of waste mother liquor composition, whole process produces without waste, environment friendly and pollution-free; The present invention adopts stage treatment mode, and the high purity protein added value of industry of preparation is high, and the feed culture efficiency of preparation is good, and the Chemical Mixed Fertilizer fertilizer efficiency of preparation is lasting, with low cost, is applicable to various crop; The present invention, in conjunction with high speed centrifugation, uv irradiating and acid hydrolysis multiple technologies, substantially increases the cleavage rate of thalline, improves protein yield; Feed of the present invention adopts ammonification and enzyme treatment technique, effectively make use of fermentation waste and agricultural waste, makes feed nutrient more comprehensively; The present invention adopts waste water to prepare fertilizer, avoids waste water resource, and provides trace element and aminoacid component, makes fertilizer nutrient comprehensive and lasting more; Present invention, avoiding by wastewater application in feed, bring hidden danger to the security of feed.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, carry out clearly, intactly describing to the present invention, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
Utilize biological organic fertilizer prepared by Methionin waste mother liquor, its preparation method comprises the steps:
1) lysine fermentation liquor is the strainer of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrated solution and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation Threonine crystal and waste liquid; Merge above-mentioned trapped substance, concentrated solution and waste liquid, obtain waste mother liquor; Wherein, microfiltration membrane is inorganic ceramic film, and molecular weight cut-off is 3000 Da, and micro-filtration temperature is 36 DEG C; Ultra-filtration membrane molecular weight cut-off is 300 Da, and ultrafiltrate temperature is 36 DEG C;
2) waste mother liquor is heated, make temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 12min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying ovens to dry to constant weight, then uviolizing 5min, thalline thickness is 10cm, and uitraviolet intensity is 2000uW/cm
2; Then thalline is added in stirred reactor, and add the hydrochloric acid soln of the 1M of double weight, 100 turns/min stirring reaction 15min, cell walls is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant liquor, be condensed into paste, finally by spray granulating fluidised bed drying, obtain albumen;
3) by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell walls merges, and natural subsidence solid-liquid separation, obtains supernatant liquor and throw out; Throw out, sorghum flour, rice chaff, egg shell powder and the wheat bran mass ratio according to 5:2:1:1:1 is mixed, add the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment water ratio to 50% massfraction, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of air seasonings, pulverize and obtain ox feed; The enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g; Described sorghum flour and egg shell powder are pulverized obtained by Chinese sorghum and Ovum crusta Gallus domesticus respectively;
4) in step 3) gained supernatant liquor, add potassium humate, diatomite, zeolite powder, dregs of beans and the peat composed of rotten mosses, stir, leave standstill 12 hours, then add ammoniacal liquor, stir, to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, monoammonium phosphate, poly aspartic acid, zinc sulfate are mixed according to the mass ratio of 200:10:1:1, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging; Wherein, the mass ratio of step 3) gained supernatant liquor, potassium humate, diatomite, zeolite powder, dregs of beans, the peat composed of rotten mosses and ammoniacal liquor is 100:30:10:8:6:6:5.
Embodiment 2
The albumen that embodiment 1 is prepared by the present invention, purity can reach more than 90%, substitutes the yeast powder in commercial standard YPD substratum, all the other components unchanged, cultivate yeast saccharomyces cerevisiae and pichia spp under the same conditions, evaluated the culture effect of product by the growing state (OD600) comparing cell.Specifically in table 1:
Table 1
Conclusion: utilize turbidimetry for Determination OD
600characterize the growing state of cell, show can substitute commercially available yeast powder product with this product, culture effect is better, can bring huge economic benefit to enterprise.
Embodiment 3
The impact that feed prepared by embodiment 1 grows for calf
Select healthy nascent calf to divide into groups in control group and test group, often organize 50, male and female half and half, measure the body weight of ox in 180 ages in days.Within after calf birth 0-10 days, feed colostrum, after the 10th day, carry out the nursing of basal diet and feed.Respectively add up calf at 30 ~ 90 days average adding weight, 120 ~ 180 days average adding weights, the 180th day chest measurement and the 180th day height four indices.Control group: basal diet: coventional type feed=8:1;
Test group: basal diet: embodiment 1 feed=8:1.The results are shown in Table 2.
The growth indexes of table 2 calf measures
| Group | Average daily gain in 30-90 days (kg/ head) | Average daily gain in 120-180 days (kg/ head) | 180th day chest measurement (cm) | 180th day height (cm) |
| Control group | 0.51 | 0.53 | 115.9 | 93.5 |
| Test group | 0.57 | 0.60 | 121.3 | 98.7 |
Conclusion: the calf speed of growth of test group is better than control group, and have obvious advantage than control group on chest measurement and height, after edible feed of the present invention is described, be beneficial to calf growth.
Embodiment 4
The field test effect of biological organic fertilizer prepared by the embodiment of the present invention 1: for wheat.
Control group: Common compound fertilizer: N-P-K content is 15: 15: 15;
Test group: biological organic fertilizer prepared by embodiment 1.
Experimental technique: select experimental plot Winter Wheat Planted, the equal 10 mu of ground of experimental plot area that two groups of fertilizer processes respectively, the usage quantity often organizing experimental plot fertilizer is 40kg, and other planting conditions are identical; Gather in the crops wheat simultaneously, measure wheat per mu yield and stimulation ratio; Detect wheat grains per spike, thousand seed weight simultaneously.Experimental result: with reference to table 3.
Table 3
| Group | Grain number per spike | Thousand seed weight (g) | Per mu yield (kg) |
| Control group | 28.9 | 45.3 | 365 |
| Test group | 31.2 | 46.9 | 387 |
Conclusion: compare discovery by table 3, test group wheat grains per spike, thousand seed weight and per mu yield, apparently higher than control group, possess statistical significance.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (6)
1. the biological organic fertilizer utilizing Methionin waste mother liquor to prepare, is characterized in that, the preparation method of described biological organic fertilizer comprises the steps:
Step 1) lysine fermentation liquor is the strainer of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrated solution and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation Threonine crystal and waste liquid; Merge above-mentioned trapped substance, concentrated solution and waste liquid, obtain waste mother liquor;
Step 2) waste mother liquor is heated, make temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 12min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying ovens to dry to constant weight, uviolizing 5min subsequently; Then thalline is added in stirred reactor, and add the hydrochloric acid soln of the 1M being equivalent to thalline double weight, 100 turns/min stirring reaction 15min, cell walls is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant liquor, be condensed into paste, finally by spray granulating fluidised bed drying, obtain albumen;
Step 3) is by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell walls merges, and natural subsidence solid-liquid separation, obtains supernatant liquor and throw out; Throw out, sorghum flour, rice chaff, egg shell powder and the wheat bran mass ratio according to 5:2:1:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment water ratio to 50%, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of air seasonings, pulverize and obtain ox feed;
Step 4) adds potassium humate, diatomite, zeolite powder, dregs of beans and the peat composed of rotten mosses in step 3) gained supernatant liquor, stirs, and leaves standstill 12 hours, then adds ammoniacal liquor, stir, and to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, monoammonium phosphate, poly aspartic acid, zinc sulfate are mixed according to the mass ratio of 200:10:1:1, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging.
2. biological organic fertilizer according to claim 1, is characterized in that, in described step 1), microfiltration membrane is inorganic ceramic film, and molecular weight cut-off is 3000Da, and micro-filtration temperature is 36 DEG C; Ultra-filtration membrane molecular weight cut-off is 300Da, and ultrafiltrate temperature is 36 DEG C.
3. biological organic fertilizer according to claim 1 and 2, is characterized in that, described ultraviolet intensity is 2000uW/cm
2.
4. biological organic fertilizer according to claim 3, is characterized in that, the enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g.
5. the biological organic fertilizer according to claim 1 or 4, is characterized in that, described sorghum flour and egg shell powder are pulverized obtained by Chinese sorghum and Ovum crusta Gallus domesticus respectively.
6. biological organic fertilizer according to claim 5, is characterized in that, in described step 4), the mass ratio of step 3) gained supernatant liquor, potassium humate, diatomite, zeolite powder, dregs of beans, the peat composed of rotten mosses and ammoniacal liquor is 100:30:10:8:6:6:5.
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| CN106010561A (en) * | 2016-06-08 | 2016-10-12 | 内蒙古阜丰生物科技有限公司 | Soil conditioning agent containing threonine fermentation waste |
| CN106045701A (en) * | 2016-06-07 | 2016-10-26 | 内蒙古阜丰生物科技有限公司 | Compound fertilizer prepared from waste through fermentation with threonine |
| CN106045626A (en) * | 2016-06-07 | 2016-10-26 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for producing biological bacterial fertilizer from amino acid fermentation liquid waste and corn stalks |
| CN106187423A (en) * | 2016-06-30 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation garbage |
| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN107337569A (en) * | 2017-09-07 | 2017-11-10 | 界首市金牛家庭农场 | A kind of preparation method of tomato planting organic fertilizer |
| CN112047519A (en) * | 2020-09-18 | 2020-12-08 | 安徽华恒生物科技股份有限公司 | Comprehensive utilization method of microbial fermentation waste liquid |
| CN112142519A (en) * | 2020-09-27 | 2020-12-29 | 浙华欣农(嘉兴)生物科技有限公司 | Utilize equipment of abandonment eggshell protein preparation amino acid calcium magnesium liquid fertilizer |
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| CN106045701A (en) * | 2016-06-07 | 2016-10-26 | 内蒙古阜丰生物科技有限公司 | Compound fertilizer prepared from waste through fermentation with threonine |
| CN106045626A (en) * | 2016-06-07 | 2016-10-26 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for producing biological bacterial fertilizer from amino acid fermentation liquid waste and corn stalks |
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| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN107337569A (en) * | 2017-09-07 | 2017-11-10 | 界首市金牛家庭农场 | A kind of preparation method of tomato planting organic fertilizer |
| CN112047519A (en) * | 2020-09-18 | 2020-12-08 | 安徽华恒生物科技股份有限公司 | Comprehensive utilization method of microbial fermentation waste liquid |
| CN112142519A (en) * | 2020-09-27 | 2020-12-29 | 浙华欣农(嘉兴)生物科技有限公司 | Utilize equipment of abandonment eggshell protein preparation amino acid calcium magnesium liquid fertilizer |
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