CN107176877A - A kind of method that mushroom culture medium is prepared based on distillers ' grains - Google Patents
A kind of method that mushroom culture medium is prepared based on distillers ' grains Download PDFInfo
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- CN107176877A CN107176877A CN201710424614.6A CN201710424614A CN107176877A CN 107176877 A CN107176877 A CN 107176877A CN 201710424614 A CN201710424614 A CN 201710424614A CN 107176877 A CN107176877 A CN 107176877A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/40—Fertilisers incorporated into a matrix
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
Abstract
The present invention relates to a kind of method that mushroom culture medium is prepared based on distillers ' grains, belong to culture medium of edible fungus technical field.After the present invention is first pre-processed distillers ' grains, steam blasting is carried out with ethanol solution, citric acid etc., collection obtains unloading material, material refrigeration, dry and crushing will be unloaded again, the particle that sieves is obtained, then by after the progress mixed ventilation such as sieving particle and maize pulp, water, and add dusty yeast progress mixed fermentation, after tunning is spray-dried, is mixed and sterilized with water, glucose etc., you can obtain mushroom culture medium.The present invention is that primary raw material prepares mushroom culture medium using distillers ' grains, substantially increases the utilization rate of distillers ' grains, while the strain rate of growth that mushroom culture medium improves mushroom is made, the growth of mushroom is not influenceed.
Description
Technical field
The present invention relates to a kind of method that mushroom culture medium is prepared based on distillers ' grains, belong to culture medium of edible fungus technology neck
Domain.
Background technology
Vinasse are the accessory substances produced in brewed spirit production process, and yield is larger, and main component is water, rice husk and grain
Residue after food fermentation, volatile organic content is high, in acidity, easily corruption, is used for animal and fowl fodder at present or directly burns
Burning processing.Because rice husk content is high in vinasse, feed quality is poor, and livestock and poultry cultivation is easily by market and seasonal effect, at vinasse
Reason brings unstability.Vinasse can not such as be handled in time, and outdoor accumulation deposit easily corruption is smelly, influences environmental sanitation, only dries
It could be deposited for a long time after dry.But grain fermentation residues toughness in vinasse, and rice husk mix, and are not readily separated, it is easy to tie
Block, hardly possible drying, and small molecule volatile organic matter of the grain fermentation residues containing higher concentration, the two factors affects are burned
Efficiency, it is difficult to burn thorough.
Traditional processing method is to be made feed, and fuel is made in the direct drying also having now.Because heavy thick fibre
Dimension hplc is too high, reaches more than 25%, does that feed quality is too poor, and brewery often can not be sold；Poor easily caking is lost in addition, is difficult to dry
It is dry, efficiency of combustion is influenceed, rice hull ash quality is influenceed.Therefore, urgent one kind of finding can at utmost utilize distillers ' grains, improve its profit
With the method for rate.
Mushroom contains high protein, low fat, polysaccharide, several amino acids and multivitamin as a kind of edible mushroom.Mushroom
With a variety of medicinal functions, such as raising body's immunity, anti-aging, cancer-resisting, lowering blood pressure and blood fat, norcholesterol,
Mushroom also to therapeutic actions such as diabetes, pulmonary tuberculosis, catarrhal jaundice, neuritis, can be used for indigestion, constipation etc. again.It is fragrant
Mushroom, may be because place sanitary condition be poor, it is not thorough to sterilize, inoculation misoperation in incubation, the reason such as packages in damaged condition,
By some living contaminantses, the reduction of strain survival rate influences the growth of mushroom, caused damage to production.How to find a kind of favourable
The mushroom culture medium of mushroom growth turns into the new demand in edible mushroom field.
The content of the invention
The technical problems to be solved by the invention：Feed is made for existing distillers ' grains, feed quality is influenceed, directly burned
Influence efficiency of combustion, it is impossible to which there is provided a kind of method that mushroom culture medium is prepared based on distillers ' grains for the problem of improving its utilization rate.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
（1）Distillers ' grains and water are stirred, stood, the impurity of water surface floating is removed, stirring mixture is filtered, collected
Screening, is dried, and collects dried object；
（2）Count by weight, take ethanol solution, 30~35 parts of dried objects, 10~15 that 60~70 parts of mass fractions are 10%
Part citric acid and 3~6 parts of sodium carbonate, are put into steam blasting device and carry out steam blasting, collect explosion thing；
（3）Explosion thing is stood at 3~5 DEG C 4~6h of freeze-drying is carried out after 10~15min, collect freeze-drying thing, will be cold
Freeze dried object to be crushed, sieve, collect sieving particle；
（4）Count by weight, take 60~65 parts of sieving particles, 20~25 parts of maize pulps, 22~24 parts of water and 12~15 portions of rice
Chaff is put into mixer and stirred, and collects stirring mixture and is put into fermentation vat, gravity-flow ventilation 5~7 days；
（5）After ventilation terminates, dusty yeast is added into fermentation vat, sealing and fermenting pond is fermented 2~4 days, and every 7~9h is with 150r/
Min stirs 4~6min, collects the fermenting mixture in fermentation vat, is spray-dried, and collects spray-dried product；
（6）Count by weight, take 60~65 parts of spray-dried products, 35~38 parts of water, 12~15 parts of glucose, 4~6 parts of urea
And 3~5 parts of gypsum are well mixed, and obtain mushroom culture medium base-material；
（7）Mushroom culture medium base-material is loaded in a diameter of 13~16cm, a length of 38~42cm sealed polyethylene plastic cylinder bag,
It is subsequently placed into steam sterilizer and sterilizes, be cooled to room temperature, collects sterilizing thing, you can obtain mushroom culture medium.
The step（1）The mass ratio of middle distillers ' grains and water is 1:3~5.
The step（2）Steam blasting pressure is 1.2~1.5MPa, and maintenance pressure time is 15~20min.
The step（3）The temperature of freeze-drying is -45~-40 DEG C.
The step（5）The addition of dusty yeast is the 1~3% of sieving granular mass.
The step（5）Fermentation temperature is 35~38 DEG C.
The present invention is compared with other method, and advantageous effects are：
（1）The present invention is that primary raw material prepares mushroom culture medium using distillers ' grains, substantially increases the utilization rate of distillers ' grains, it is to avoid
Distillers ' grains pollute the generation of environmental phenomenons；
（2）The mushroom culture medium that the present invention is prepared using distillers ' grains improves the survival rate of strain, is conducive to the growth of mycelia, carries
The high yield of mushroom；
（3）Distillers ' grains are carried out carry out steam blasting, expandable pores rate by the present invention, while carrying out acid to hole using citric acid
Change, increase later stage fermentation efficiency, while adding sodium carbonate in blasting process, on the one hand improve porosity, two be to distillers ' grains
Interior lipid is removed, and obtains high-nutrient, then it is carried out into mixed fermentation with maize pulp, water etc., supplements not enough nutrition
Thing, is balanced to the distribution of nutrient overall in mushroom culture medium, improves bioactivity.
Embodiment
In mass ratio 1:3~5, take distillers ' grains and water to be put into mixer, 10~15min is stirred with 150r/min, then it is quiet
20~30min is put, the impurity of water surface floating is removed, stirring mixture is filtered, filtrate is collected and is put into 60 DEG C of dryings
4~6h is dried in case, dried object is collected；Count by weight, take 60~70 parts of mass fractions be 10% ethanol solution, 30~
35 parts of above-mentioned dried objects, 10~15 parts of citric acids and 3~6 parts of sodium carbonate, are put into steam blasting device, set pressure as 1.2
~1.5MPa, maintains 15~20min of pressure, and tank is put in subsequent pressure release, collects explosion thing；Explosion thing is put into quiet in 3 DEG C of refrigerating chambers
Put and be put into after 10~15min in freeze drier, 4~6h of freeze-drying is carried out in the case where temperature is -45~-40 DEG C, freezing is collected
Dried object, freeze-drying thing is put into pulverizer and crushed, 100 mesh sieves are crossed, and collects sieving particle；Count by weight,
60~65 parts of above-mentioned sieving particles, 20~25 parts of maize pulps, 22~24 parts of water and 12~15 parts of rice chaff are taken to be put into mixer, with
200r/min stirs 10~15min, collects stirring mixture and is put into fermentation vat, gravity-flow ventilation 5~7 days；In above-mentioned ventilation knot
Shu Hou, the above-mentioned dusty yeast of sieving granular mass 1~3% is added into fermentation vat, sealing and fermenting pond keeps the temperature in fermentation vat
For 35~38 DEG C, ferment 2~4 days, every 7~9h stirs 4~6min with 150r/min；After above-mentioned stirring terminates, fermentation is collected
Fermenting mixture in pond is spray-dried, and is collected spray-dried product, is counted by weight, take 60~65 parts of spray drying
Thing, 35~38 parts of water, 12~15 parts of glucose, 4~6 parts of urea and 3~5 parts of gypsum are well mixed, and obtain mushroom culture medium base-material；
Above-mentioned mushroom culture medium base-material is loaded in a diameter of 13~16cm, a length of 38~42cm sealed polyethylene plastic cylinder bag, with
After be put into steam sterilizer, sterilize 2~3h at 105~110 DEG C, naturally cools to room temperature, collects sterilizing thing, you can it is fragrant
Mushroom culture medium.
Example 1
In mass ratio 1:5, take distillers ' grains and water to be put into mixer, 15min is stirred with 150r/min, then 30min is stood, remove
The impurity of water surface floating, is filtered to stirring mixture, is collected filtrate and is put into 60 DEG C of drying boxes dry 6h, collects dry
Dry thing；Count by weight, take ethanol solution, 35 parts of above-mentioned dried objects, 15 parts of citric acids and 6 that 70 parts of mass fractions are 10%
Part sodium carbonate, is put into steam blasting device, sets pressure as 1.5MPa, maintains pressure 20min, and tank is put in subsequent pressure release, is collected
Explosion thing；Explosion thing is put into after standing 15min in 3 DEG C of refrigerating chambers and be put into freeze drier, carried out in the case where temperature is -40 DEG C
6h is freeze-dried, freeze-drying thing is collected, freeze-drying thing is put into pulverizer and crushed, 100 mesh sieves are crossed, sieving is collected
Particle；Count by weight, take 65 parts of above-mentioned sieving particles, 25 parts of maize pulps, 24 parts of water and 15 parts of rice chaff to be put into mixer,
15min is stirred with 200r/min, stirring mixture is collected and is put into fermentation vat, gravity-flow ventilation 7 days；After above-mentioned ventilation terminates,
The above-mentioned dusty yeast of sieving granular mass 3% is added into fermentation vat, sealing and fermenting pond keeps the temperature in fermentation vat for 38 DEG C,
Fermentation 4 days, 6min is stirred per 9h with 150r/min；After above-mentioned stirring terminates, the fermenting mixture collected in fermentation vat is carried out
Spray drying, collects spray-dried product, counts by weight, takes 65 parts of spray-dried products, 38 parts of water, 15 parts of glucose, 6 parts of urine
Element and 5 parts of gypsum are well mixed, and obtain mushroom culture medium base-material；Above-mentioned mushroom culture medium base-material is loaded into a diameter of 16cm, it is a length of
In 42cm sealed polyethylene plastic cylinder bag, it is subsequently placed into steam sterilizer, sterilize 3h at 110 DEG C, naturally cools to room
Temperature, collects sterilizing thing, you can obtain mushroom culture medium.
Example 2
In mass ratio 1:3, take distillers ' grains and water to be put into mixer, 10min is stirred with 150r/min, then 20min is stood, remove
The impurity of water surface floating, is filtered to stirring mixture, is collected filtrate and is put into 60 DEG C of drying boxes dry 4h, collects dry
Dry thing；Count by weight, take ethanol solution, 30 parts of above-mentioned dried objects, 10 parts of citric acids and 3 that 60 parts of mass fractions are 10%
Part sodium carbonate, is put into steam blasting device, sets pressure as 1.2MPa, maintains pressure 15min, and tank is put in subsequent pressure release, is collected
Explosion thing；Explosion thing is put into after standing 10min in 3 DEG C of refrigerating chambers and be put into freeze drier, carried out in the case where temperature is -45 DEG C
4h is freeze-dried, freeze-drying thing is collected, freeze-drying thing is put into pulverizer and crushed, 100 mesh sieves are crossed, sieving is collected
Particle；Count by weight, take 60 parts of above-mentioned sieving particles, 20 parts of maize pulps, 22 parts of water and 12 parts of rice chaff to be put into mixer,
10min is stirred with 200r/min, stirring mixture is collected and is put into fermentation vat, gravity-flow ventilation 5 days；After above-mentioned ventilation terminates,
The above-mentioned dusty yeast of sieving granular mass 1% is added into fermentation vat, sealing and fermenting pond keeps the temperature in fermentation vat for 35 DEG C,
Fermentation 2 days, 4min is stirred per 7h with 150r/min；After above-mentioned stirring terminates, the fermenting mixture collected in fermentation vat is carried out
Spray drying, collects spray-dried product, counts by weight, takes 60 parts of spray-dried products, 35 parts of water, 12 parts of glucose, 4 parts of urine
Element and 3 parts of gypsum are well mixed, and obtain mushroom culture medium base-material；Above-mentioned mushroom culture medium base-material is loaded into a diameter of 13cm, it is a length of
In 38cm sealed polyethylene plastic cylinder bag, it is subsequently placed into steam sterilizer, sterilize 2h at 105 DEG C, naturally cools to room
Temperature, collects sterilizing thing, you can obtain mushroom culture medium.
Example 3
In mass ratio 1:4, take distillers ' grains and water to be put into mixer, 12min is stirred with 150r/min, then 25min is stood, remove
The impurity of water surface floating, is filtered to stirring mixture, is collected filtrate and is put into 60 DEG C of drying boxes dry 5h, collects dry
Dry thing；Count by weight, take ethanol solution, 32 parts of above-mentioned dried objects, 12 parts of citric acids and 5 that 65 parts of mass fractions are 10%
Part sodium carbonate, is put into steam blasting device, sets pressure as 1.3MPa, maintains pressure 17min, and tank is put in subsequent pressure release, is collected
Explosion thing；Explosion thing is put into after standing 13min in 3 DEG C of refrigerating chambers and be put into freeze drier, carried out in the case where temperature is -42 DEG C
5h is freeze-dried, freeze-drying thing is collected, freeze-drying thing is put into pulverizer and crushed, 100 mesh sieves are crossed, sieving is collected
Particle；Count by weight, take 62 parts of above-mentioned sieving particles, 22 parts of maize pulps, 23 parts of water and 13 parts of rice chaff to be put into mixer,
12min is stirred with 200r/min, stirring mixture is collected and is put into fermentation vat, gravity-flow ventilation 6 days；After above-mentioned ventilation terminates,
The above-mentioned dusty yeast of sieving granular mass 2% is added into fermentation vat, sealing and fermenting pond keeps the temperature in fermentation vat for 36 DEG C,
Fermentation 3 days, 5min is stirred per 8h with 150r/min；After above-mentioned stirring terminates, the fermenting mixture collected in fermentation vat is carried out
Spray drying, collects spray-dried product, counts by weight, takes 62 parts of spray-dried products, 36 parts of water, 13 parts of glucose, 5 parts of urine
Element and 4 parts of gypsum are well mixed, and obtain mushroom culture medium base-material；Above-mentioned mushroom culture medium base-material is loaded into a diameter of 15cm, it is a length of
In 40cm sealed polyethylene plastic cylinder bag, it is subsequently placed into steam sterilizer, sterilize 2h at 107 DEG C, naturally cools to room
Temperature, collects sterilizing thing, you can obtain mushroom culture medium.
Reference examples：Sawdust medium, by mass percentage, 68% wood chip, 30% rice bran, 1% sucrose and 1% gypsum mixing and
Into.
Test method：The culture dish for selecting four specifications consistent, then respectively prepare example 1 to example 2 and reference examples
Mushroom culture medium is placed in culture dish, and each inoculation of medium mushroom strain in culture dish, and the amount of inoculation is 12%, is connect
Kind terminate week, in temperature be 22 DEG C, relative humidity be 65% under the conditions of cultivated, daily observation mycelia growing state, remember
The sprout time of mycelia is recorded, the data such as the speed of growth detect data such as table 1.
Table 1
| Culture medium | Sprout time/h | Average growth rate/（mm/d） | Growing way situation | Sturdy situation |
| Reference examples | 16.5 | 3.6 | Mycelial growth potential is weak | It is very thin |
| Example 1 | 13.2 | 4.7 | Mycelial growth potential is strong | It is sturdy |
| Example 2 | 14.1 | 4.9 | Mycelial growth potential is strong | It is sturdy |
| Example 3 | 14.2 | 5.1 | Mycelial growth potential is strong, close | It is sturdy |
The mushroom culture medium that the present invention is prepared using distillers ' grains as can be seen from Table 1 may advantageously facilitate the growth of mushroom, be beneficial to carry
Its high yield.
Claims (6)
1. a kind of method that mushroom culture medium is prepared based on distillers ' grains, it is characterised in that specifically preparation process is：
（1）Distillers ' grains and water are stirred, stood, the impurity of water surface floating is removed, stirring mixture is filtered, collected
Screening, is dried, and collects dried object；
（2）Count by weight, take ethanol solution, 30~35 parts of dried objects, 10~15 that 60~70 parts of mass fractions are 10%
Part citric acid and 3~6 parts of sodium carbonate, are put into steam blasting device and carry out steam blasting, collect explosion thing；
（3）Explosion thing is stood at 3~5 DEG C 4~6h of freeze-drying is carried out after 10~15min, collect freeze-drying thing, will be cold
Freeze dried object to be crushed, sieve, collect sieving particle；
（4）Count by weight, take 60~65 parts of sieving particles, 20~25 parts of maize pulps, 22~24 parts of water and 12~15 portions of rice
Chaff is put into mixer and stirred, and collects stirring mixture and is put into fermentation vat, gravity-flow ventilation 5~7 days；
（5）After ventilation terminates, dusty yeast is added into fermentation vat, sealing and fermenting pond is fermented 2~4 days, and every 7~9h is with 150r/
Min stirs 4~6min, collects the fermenting mixture in fermentation vat, is spray-dried, and collects spray-dried product；
（6）Count by weight, take 60~65 parts of spray-dried products, 35~38 parts of water, 12~15 parts of glucose, 4~6 parts of urea
And 3~5 parts of gypsum are well mixed, and obtain mushroom culture medium base-material；
（7）Mushroom culture medium base-material is loaded in a diameter of 13~16cm, a length of 38~42cm sealed polyethylene plastic cylinder bag,
It is subsequently placed into steam sterilizer and sterilizes, be cooled to room temperature, collects sterilizing thing, you can obtain mushroom culture medium.
2. a kind of method that mushroom culture medium is prepared based on distillers ' grains according to claim 1, it is characterised in that：The step
Suddenly（1）The mass ratio of middle distillers ' grains and water is 1:3~5.
3. a kind of method that mushroom culture medium is prepared based on distillers ' grains according to claim 1, it is characterised in that：The step
Suddenly（2）Steam blasting pressure is 1.2~1.5MPa, and maintenance pressure time is 15~20min.
4. a kind of method that mushroom culture medium is prepared based on distillers ' grains according to claim 1, it is characterised in that：The step
Suddenly（3）The temperature of freeze-drying is -45~-40 DEG C.
5. a kind of method that mushroom culture medium is prepared based on distillers ' grains according to claim 1, it is characterised in that：The step
Suddenly（5）The addition of dusty yeast is the 1~3% of sieving granular mass.
6. a kind of method that mushroom culture medium is prepared based on distillers ' grains according to claim 1, it is characterised in that：The step
Suddenly（5）Fermentation temperature is 35~38 DEG C.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107500904A (en) * | 2017-09-25 | 2017-12-22 | 常州苏通海平机电科技有限公司 | A kind of preparation method of Mushroom cultivation material |
| CN107500917A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method of more grain aroma type spirit distiller grain Hericium erinaceus special culture medias |
| CN107822114A (en) * | 2017-11-07 | 2018-03-23 | 常州汉唐文化传媒有限公司 | A kind of preparation method of meal replacement powder |
| CN108901619A (en) * | 2018-08-23 | 2018-11-30 | 安徽农耕年华农业发展有限公司 | A kind of mushroom culture medium and preparation method thereof |
| CN111778166A (en) * | 2020-08-21 | 2020-10-16 | 四川润格生物科技有限公司 | Technical method for producing aspergillus niger by using white spirit vinasse |
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| CN103232298A (en) * | 2013-05-16 | 2013-08-07 | 贵州大学 | Substrate for distillers' grain cultivated coprinus comatus and manufacturing method thereof |
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| CN103373871A (en) * | 2012-04-12 | 2013-10-30 | 天津市绿色坐标生态科技有限公司 | Vinasse-containing edible fungus culture medium and preparation method |
| CN103787712A (en) * | 2014-03-11 | 2014-05-14 | 南通禾宝生物科技有限公司 | Method for preparing organic fertilizer |
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| CN103373871A (en) * | 2012-04-12 | 2013-10-30 | 天津市绿色坐标生态科技有限公司 | Vinasse-containing edible fungus culture medium and preparation method |
| CN103232298A (en) * | 2013-05-16 | 2013-08-07 | 贵州大学 | Substrate for distillers' grain cultivated coprinus comatus and manufacturing method thereof |
| CN103332992A (en) * | 2013-07-18 | 2013-10-02 | 司天戎 | High-quality pleurotus eryngii culture medium and preparation method |
| CN103787712A (en) * | 2014-03-11 | 2014-05-14 | 南通禾宝生物科技有限公司 | Method for preparing organic fertilizer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107500904A (en) * | 2017-09-25 | 2017-12-22 | 常州苏通海平机电科技有限公司 | A kind of preparation method of Mushroom cultivation material |
| CN107500917A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method of more grain aroma type spirit distiller grain Hericium erinaceus special culture medias |
| CN107822114A (en) * | 2017-11-07 | 2018-03-23 | 常州汉唐文化传媒有限公司 | A kind of preparation method of meal replacement powder |
| CN108901619A (en) * | 2018-08-23 | 2018-11-30 | 安徽农耕年华农业发展有限公司 | A kind of mushroom culture medium and preparation method thereof |
| CN111778166A (en) * | 2020-08-21 | 2020-10-16 | 四川润格生物科技有限公司 | Technical method for producing aspergillus niger by using white spirit vinasse |
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Application publication date: 20170919 |