CN103901189B - Immune detection micro-fluidic chip fluorescent quantitation automatic detection device - Google Patents
Immune detection micro-fluidic chip fluorescent quantitation automatic detection device Download PDFInfo
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Abstract
The invention discloses a kind of immune detection micro-fluidic chip fluorescent quantitation automatic detection device, comprise excitation source, exciting light focus module, phosphor collection module, photoelectric conversion module, linear movement console module, circuit module, reaction automatically performs module, waste reaction solution removes module.In testing process, the line spot obtained by exciting light focus module is implemented one dimension quantitative scanning to chip and is detected.Controller, based on the fluorescence signal intensity distributed intelligence in chip two dimensional rule detection region, obtains testing sample concentration or number of cells.Testing result connects mobile phone by bluetooth and is sent to medical institutions in real time, realizes remote diagnosis or the monitoring of epidemic disease distributed areas.The present invention adopts line spot one-dimensional scanning detection method, achieves the quantification that micro-fluidic chip detects; Rely on reaction to automatically perform module, realize the robotization that micro-fluidic chip detects, and remove module by integrated waste reaction solution and increase the response sample amount of allowing, improve detection sensitivity.
Description
Technical field
The present invention relates to field of biological detection, particularly a kind of immune detection micro-fluidic chip fluorescent quantitation automatic detection device.
Background technology
Immune analysis method utilizes the specific binding of antigen-antibody to react the analytical approach detecting all kinds of target (medicine, hormone, protein, microorganism etc.).Based on the micro-fluidic chip method for quick of immune detection principle and device low, convenient and swift owing to having cost, and the feature such as simple to operate, in quick diagnosis or bedside diagnostic field, there is significant advantage, just becoming the medical diagnosis platform of future generation that countries in the world are competitively developed.Fluorescence detection method has the feature of high sensitivity and wide dynamic range, effectively can improve the combination property of micro-fluidic chip immune detection.
Existing fluorescent quantitative detector is often merely able to read test result, and automatically cannot complete the multiple operation stepss included by complicated micro-fluidic chip immunoassay procedures, this limits its range of application to a certain extent.In addition, detection method of microfluidic chip is by its homologation reaction sample quantitative limitation, and detection sensitivity is limited.
Adopt the single luminous point that excites to carry out the fluorescence detection device of one-dimensional scanning detection, principle is said, this is similar to the detection line having drawn a band sampling property with certain position exciting luminous point at micro-fluidic chip, therefore, be merely able to obtain the fluorescence signal on this sampling Detection line, and the method complete detection signal strength distribution cannot be obtained, so can only realize qualitative detection.
In order to realize the immunoreactive quantitative detection of micro-fluidic chip, as detected the target protein concentration in sample, or realize the counting of target cell in blood, in theory, the complete fluorescence signal intensity distributed intelligence (comprising the amplitude of signal and the physical boundary of signal) of accurately extracting conversion zone on micro-fluidic chip is needed.The two-dimensional fluoroscopic scanning detection method of bibliographical information, by implementing the two-dimensional scan of pointwise to surveyed area, complete fluorescence signal intensity distribution can be obtained thus, but, it is long to there is detection system complexity, complex operation, detection time in spot scan detection method, and the more high deficiency of cost.
Summary of the invention
The object of this invention is to provide the immune detection micro-fluidic chip fluorescent quantitation automatic detection device that a kind of structure is simple, easy to operate, highly sensitive.The linear electric motors utilizing reaction to automatically perform in module complete complicated immune detection reaction automatically to drive the liquid storage Micropump on micro-fluidic chip.Meanwhile, utilize waste reaction solution to remove miniature electric resistance heater integrated in module to realize the contact spot heating of micro-fluidic chip, remove waste reaction solution by evaporation mode, that improves chip detection allows sample size, improves detection sensitivity thus.Read to realize quantitative detection signal, based on total differential sampling Detection principle, continuous sweep micro-fluidic chip is carried out by designing fine linear exciting light spot, simultaneously, the fluorescence signal of continuous acquisition micro-fluidic chip is carried out by highly sensitive photodiode, thus, achieve in micro-fluidic chip detection reaction region, the extraction of high resolving power (spacing of neighbouring sample point reaches a μm level) fluorescence signal intensity distribution, the algorithm recycling controller built-in extrapolates testing sample concentration, or number of cells.By the extraneous interface module in circuit module, the function such as transmission, printout of data can be realized easily, be user-friendly to.Especially, the Bluetooth wireless communication module that can be comprised by circuit module, is sent to medical diagnosis mechanism by testing result in real time by smart mobile phone, realizes remote diagnosis or the monitoring of epidemic disease distributed areas.
Specifically, the invention provides a kind of immune detection micro-fluidic chip fluorescent quantitation automatic detection device, comprise: reaction automatically performs module, waste reaction solution removes module, excitation source, exciting light focus module, phosphor collection module, linear movement console module, circuit module, and circuit module comprises: master controller, submaster controller and extraneous interface module.
In immune detection course of reaction, by the control of circuit module, the reaction one or more linear electric motors (mini drive permutation) automatically performed in module drive the reaction liquid storage Micropump of micro-fluidic chip, all kinds of reaction reagent is driven into relevant conversion zone, automatically completes detection reaction.Electronic journey switch is adopted to realize linear electric motors motion positions.
In immune detection course of reaction, in order to the response sample amount of being allowed by increase, reach the object improving detection sensitivity, by the control of circuit module, allow waste reaction solution remove miniature electric resistance heater integrated in module to realize the contact spot heating of micro-fluidic chip, remove waste reaction solution by evaporation mode.The working temperature of miniature electric resistance heater is detected by the micro thermocouple temperature sensor being fixed on its surface, and feeds back to the closed-loop control that controller realizes ON/OFF pattern.
After immune response completes, in testing result reading process, the original hot spot that excites that excitation source produces is assembled for the linear hot spot that excites through exciting light focus module, in order to improve the spatial resolution of detection signal, the physical boundary in accurate recognition detection signal distributions region, improve detection accuracy, the width of line spot should enough carefully (as: 50-250 μm), simultaneously, in order to obtain complete micro-fluidic chip fluorescence signal distribution, the length of line spot should be close with the cross-sectional width in micro-fluidic chip two dimension rule detection region.In order to improve the Scanning Detction precision of micro-fluidic immune detection chip, realize quantitatively detecting accurately, utilize the micro-stepping control technology of stepper motor, pass through belt drive system, realize at the uniform velocity micro-fluidic chip linear movement, make the physical location spacing of neighbouring sample point reach μm rank to improve the resolution of Scanning Detction.The fluorescence that micro-fluidic chip produces enters phosphor collection module, is converged by lens wherein, the highly sensitive photoelectric conversion module of final arrival.Submaster controller continuous acquisition photoelectric conversion signal, and send it to master controller, based on the fluorescence signal intensity distributed intelligence that Scanning Detction obtains, extrapolate quantitative testing result (sample concentration or number of cells) according to built-in analytical algorithm and mathematical model.
In order to reduce installation cost, have employed LED, and nonideal pointolite---laser, therefore, in order to improve detection resolution, the present invention needs to design unique exciting light focus module, by cooperatively interacting between multiple condenser lens, cylindrical mirror, obtains scanning the linear of micro-fluidic chip and excites hot spot.
By the Bluetooth wireless communication module in extraneous interface module, the exchanges data between this pick-up unit and PC, smart mobile phone can be realized.On the one hand, can in examinations process, PC or smart mobile phone are to the control of pick-up unit, on the other hand, testing result can be transferred to PC, or smart mobile phone, then send to all kinds of medical diagnosis mechanism by the mobile network communication technology such as GPRS network of Internet network or mobile phone, realizes intelligent, informationalized remote diagnosis or the monitoring of epidemic disease distributed areas.
Preferably, in course of reaction, the reaction liquid storage Micropump automatically performed on module drive micro-fluidic chip completes immune detection reaction automatically.The linear electric motors reacting automatic driver module by electronic journey switch to realize positioning control.
Preferably, in course of reaction, waste reaction solution removes microheater integrated in module and removes sample waste by evaporation mode, increases the application of sample amount of allowing of response sample to improve detection sensitivity.The micro thermocouple temperature sensor of temperature through its surface of microheater detects and feeds back to submaster controller and realize ON/OFF closed-loop control.
Preferably, exciting light projects on micro-fluidic chip after exciting light focus module, and the fluorescence that micro-fluidic chip excites receives through phosphor collection module, then is converted to electric signal, finally handled by circuit module by photoelectric conversion module (photodiode).
Preferably, the relative position of exciting light focus module and linear movement console module can regulate, and the relative position of phosphor collection module and linear movement console module also can regulate, and can realize the micro-fluidic chip Scanning Detction of high flexibility ratio thus.
Preferably, this device also comprises the high-pass filter (22) be arranged between phosphor collection module (5) and photoelectric conversion module (6), high-pass filter (22) filter wavelength is greater than the wavelength of exciting light, and the bandpass filter (21) be arranged between excitation source (1) and exciting light focus module (2), after bandpass filter (21) filters, wavelength is at excitation wavelength ± 35nm, is respectively used to entering the fluorescence of photoelectric conversion module (6) and filtering the exciting light entering exciting light focus module (2).
Preferably, linear movement console module comprises stepper motor, driving belt and the draw-in groove fixing with driving belt, wherein draw-in groove is for placing detected micro-fluidic chip, when stepper motor rotates under the driving of submaster controller, belt, draw-in groove and micro-fluidic chip is wherein driven to move linearly.
Preferably, master controller is used for analysis detecting data, and according to built-in mathematical model, quantitatively calculates the concentration value detecting sample, or the number of cells of testing sample.
Preferably, light source is LED, and submaster controller can control the on off state of excitation source, and digitizing can arrange constant current source size to regulate the brightness of light source, obtains optimum detection signal-to-noise ratio.
Preferably, in circuit module, comprise Bluetooth wireless communication module, for realizing the exchanges data between this pick-up unit and PC, smart mobile phone.
Description of the invention provides in order to example with for the purpose of describing, and is not exhaustively or limit the invention to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Selecting and describing embodiment is in order to principle of the present invention and practical application are better described, and enables those of ordinary skill in the art understand the present invention thus design the various embodiments with various amendment being suitable for special-purpose.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.Apparently, the accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the schematic diagram of an immune detection micro-fluidic chip fluorescent quantitation automatic detection device of the present invention embodiment.
Fig. 2 is the schematic diagram of another embodiment of immune detection micro-fluidic chip fluorescent quantitation automatic detection device of the present invention.
Fig. 3 is the inner structure schematic diagram of an embodiment of exciting light focus module of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described.Obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.In fact be only illustrative to the description of at least one exemplary embodiment below, never as any restriction to the present invention and application or use.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
Unless specifically stated otherwise, otherwise positioned opposite, the numerical expression of the parts of setting forth in these embodiments and step and numerical value do not limit the scope of the invention.
Meanwhile, should be clear and definite, for convenience of description, the size of the various piece shown in accompanying drawing is not draw according to the proportionate relationship of reality.
May not discuss in detail for the known technology of person of ordinary skill in the relevant, method and apparatus, but in the appropriate case, described technology, method and apparatus should be regarded as a part of authorizing instructions.
In all examples with discussing shown here, any occurrence should be construed as merely exemplary, instead of as restriction.Therefore, other example of exemplary embodiment can have different values.
It should be noted that: represent similar terms in similar label and letter accompanying drawing below, therefore, once be defined in an a certain Xiang Yi accompanying drawing, then do not need to be further discussed it in accompanying drawing subsequently.
Fig. 1 is the schematic diagram of an immune detection micro-fluidic chip fluorescent quantitation automatic detection device of the present invention embodiment.As shown in Figure 1, immune detection micro-fluidic chip fluorescent quantitation automatic detection device comprises excitation source (1), exciting light focus module (2), linear movement console module (3), phosphor collection module (5), photoelectric conversion module (6), circuit module (7), waste reaction solution removes module (8), reaction automatically performs module (9), and circuit module (7) comprises master controller (71), submaster controller (72) and extraneous interface module (73).
In course of reaction, by the control of circuit module 7, the reaction one or more linear electric motors (mini drive permutation) automatically performed in module 9 drive the reaction liquid storage Micropump of micro-fluidic chip, all kinds of reaction reagent is driven into relevant conversion zone, automatically completes detection reaction.
In course of reaction, by the control of circuit module 7, allow waste reaction solution remove miniature electric resistance heater integrated in module 8 to realize the contact spot heating of micro-fluidic chip, remove waste reaction solution by evaporation mode, that improves response sample allows application of sample amount.
In testing result reading process, excitation source 1 produces exciting light, exciting light is positioned on the micro-fluidic chip 4 of linear movement platform 3 by focusing on after exciting light focus module 2, linear movement platform 3 drives micro-fluidic chip 4 to move, and achieves the scanning of exciting light to micro-fluidic chip two dimension rule detection region.
In micro-fluidic chip 4 motion process, on micro-fluidic chip 4 in two-dimentional rule detection region the fluorescent marker of ad-hoc location excite by linear exciting light, the fluorescence produced is collected by phosphor collection module 5, and the highly sensitive photoelectric conversion module 6 of final arrival, light signal is converted to electric signal.In Scanning Detction process, the electric signal that submaster controller 72 continuous acquisition photoelectric conversion module 6 obtains, and be sent to master controller 71, master controller 71 utilizes built-in data processing algorithm to carry out data analysis, and accurately calculate testing sample concentration, or the target cell number in sample.By the Bluetooth wireless communication module that extraneous interface module 73 comprises, testing result can be transferred to PC, or smart mobile phone, send to all kinds of medical diagnosis mechanism by the mobile network communication technology such as GPRS network of Internet network or mobile phone again, realize intelligent, informationalized remote diagnosis or the monitoring of epidemic disease distributed areas.
Based on the immune detection micro-fluidic chip fluorescent quantitation automatic detection device that the above embodiment of the present invention provides, automatically perform module by reaction and automatically complete immune detection reaction, remove module by waste reaction solution to increase and immunoreactively allow application of sample amount, the extraction on chip detection region fluorescence signal intensity and border is achieved eventually through one dimension line spot Scanning Detction pattern, and utilize built-in algorithm to extrapolate testing sample concentration, or number of cells.The present invention, on the basis that fluorescent quantitation detects, achieves the immunoreactive automation mechanized operation of micro-fluidic chip, and the response sample amount of allowing by increasing chip improves detection sensitivity.
Preferably, reaction automatically performs module 9 and adopts linear electric motors to drive the liquid storage Micropump of micro-fluidic chip, and linear electric motors realize position by electronic journey switch and detect, and achieve immunoreactive automation mechanized operation on micro-fluidic chip.
Preferably, waste reaction solution removes module 8 and is integrated with microheater and removes waste reaction solution by evaporation profiles, working temperature is detected by the micro thermocouple temperature sensor being fixed on its surface and feeds back to the submaster controller 71 in circuit module 7, realizes the closed-loop control based on ON/OFF pattern.
Preferably, excitation source 1 is LED(LightEmittingDiode, light emitting diode).In a preferred embodiment, that the excitation wavelength that light source 1 provides is selected is 365nm.
Preferably, produce fluorescence wavelength be 615nm.
Preferably, the photoelectric conversion module adopted is photodiode (sensitivity: 145V/ μ W, near wavelength 600nm).
Preferably, controlled the on off state of excitation source 1 by submaster controller 72, and constant current source size can be set digitizing to regulate the brightness of light source, obtain optimum testing result.
Preferably, linear movement console module 3 comprises stepper motor, driving belt and the draw-in groove fixing with driving belt, wherein draw-in groove is for placing micro-fluidic chip 4, when stepper motor rotates under the driving of submaster controller 72, belt, draw-in groove and micro-fluidic chip wherein 4 is driven to move linearly, simultaneously, under the effect of linear exciting light spot, complete the fluorescence signal acquisition in micro-fluidic chip two dimension rule detection region, finally obtain complete fluorescence signal intensity distribution, and extrapolate testing sample concentration thus, or the target cell number in sample.
Preferably, exciting light enters into exciting light focus module 2, first the condenser lens 201 in exciting light focus module 2 converges to a bit, by cylindrical mirror 202, exciting light is made to be converted to the line spot of scanning micro-fluidic chip, fluorescence excitation label on ad-hoc location in the two-dimentional rule detection region that this line spot projects linear stage micro-fluidic chip.
Preferably, extraneous interface module 73 comprises Bluetooth wireless communication module, for realizing the exchanges data between this pick-up unit and PC, smart mobile phone.
In one embodiment, extraneous interface module 73 can realize the communication with the external world.
Preferably, extraneous interface module 73 can comprise for carrying out the USB(UniversalSerialBus of data transmission, USB (universal serial bus) with external computing machine) interface.
Preferably, extraneous interface module 73 can comprise the printer interface for external printer, external printer printable go out final testing result.
Preferably, extraneous interface module 73 can comprise for carrying out the network port of real-time Communication for Power with external system.
Preferably, extraneous interface module 73 can comprise the display interface for external-connection displayer.
Fig. 2 is the schematic diagram of another embodiment of immune detection micro-fluidic chip fluorescent quantitation automatic detection device of the present invention.Compared with embodiment illustrated in fig. 1, in the embodiment depicted in figure 2, also comprise the bandpass filter 21 be arranged between excitation source 1 and exciting light focus module 2, for filtering the exciting light entering exciting light focus module 2.
The effect arranging bandpass filter 21 only allows the light meeting specific wavelength condition to pass through.Preferably, bandpass filter 21 allows the wavelength coverage of the light passed through to be 320-390nm, and the exciting light being namely mainly used in allowing excitation source 1 to produce passes through.
Preferably, in the embodiment depicted in figure 2, the high-pass filter 22 be arranged between phosphor collection module 5 and photoelectric conversion module 6 is also comprised, for filtering the fluorescence entering photoelectric conversion module 6.
The effect arranging high-pass filter 22 only allows the light meeting specific wavelength condition to pass through.Preferably, high-pass filter 22 allows the wavelength coverage of the light passed through for being greater than 580nm, and the exciting light being namely mainly used in avoiding light source 1 to produce enters phosphor collection module 5.
For Fig. 2, the present invention will be described below.
In immune detection course of reaction, by the control of circuit module, the reaction linear electric motors automatically performed in module drive the liquid storage Micropump of micro-fluidic chip, automatically complete immune detection reaction.
In immune detection course of reaction, by the control of circuit module, the microheater that waste reaction solution removes in module removes waste reaction solution by evaporation mode, increases the detection sample pipetting volume amount of allowing, improves detection sensitivity.
In testing result reading process, excitation source 1 produces exciting light, and exciting light, by after the filtration of bandpass filter 21, enters exciting light focus module 2, only to allow the light meeting respective wavelength requirement to enter exciting light focus module 2.Exciting light is placed on the micro-fluidic chip 4 of linear stage 3 by converging to after exciting light focus module, and linear stage 3 drives micro-fluidic chip 4 to move, and exciting light can carry out Scanning Detction to whole micro-fluidic chip thus.
Rear generation fluorescence is penetrated in the illumination that is excited of fluorescent marker on micro-fluidic chip 4, fluorescence arrives high-pass filter 22 by the lens in phosphor collection module 5, after high-pass filter 22 filters, arrive photoelectric conversion module 6, thus only allow the light (namely exciting the fluorescence of generation) meeting respective wavelength requirement to arrive photoelectric conversion module 6.
Light signal is converted to electric signal after photoelectric conversion module 6, in collected submaster controller 72, signal is sent in master controller 71 and is analyzed by submaster controller 72, and the testing result of final quantitative (detect sample concentration or detect target cell number in sample).By the Bluetooth wireless communication module that extraneous interface module 73 comprises, testing result can be transferred to PC, or smart mobile phone, send to all kinds of medical diagnosis mechanism by the mobile network communication technology such as GPRS network of Internet network or mobile phone again, realize intelligent, informationalized remote diagnosis or the monitoring of epidemic disease distributed areas.
Fig. 3 is the inner structure schematic diagram of an embodiment of exciting light focus module of the present invention.Exciting light focus module 2 comprises condenser lens 201 and cylindrical mirror 202, and condenser lens 201 is for converging at a luminous point by exciting light, this focal spot is converted to the line spot of scanning micro-fluidic chip by cylindrical mirror 202 again.
By implementing the present invention, following beneficial effect can be obtained:
1, in the present invention, module is automatically performed by introducing reaction, drive the liquid storage Micropump of micro-fluidic chip, automatically complicated immunoreactive multiple operation steps is completed, as detected sample amounts sample introduction, release washing lotion, label etc., achieve the automation mechanized operation of immune detection reaction on micro-fluidic chip, improve detection efficiency and repeatability, avoid makeing mistakes of manual operation.
2, in the present invention, removing module by introducing waste reaction solution, adopting evaporation mode automatically to remove waste reaction solution, thereby increase the detection sample pipetting volume amount of allowing, by in detection reaction process, in bulk sample, the enrichment effect of label to be measured, improves detection sensitivity.
3, in the present invention, exciting light can be converted to the line spot of scanning micro-fluidic chip after exciting light focus module.By submaster controller, the accurate motion of stepper motor is controlled, achieve the linear movement of micro-fluidic chip, under the effect of linear exciting light spot, complete the fluorescence signal acquisition in micro-fluidic chip two dimension rule detection region, finally obtain complete fluorescence signal intensity distribution, and extrapolate testing sample concentration thus, or detect target cell number in sample.The present invention, while ensure that accuracy in detection and sensitivity, have employed based on the linear one-dimensional scanning detection mode exciting hot spot, significantly reduces system complexity, simplify testing process.
One of ordinary skill in the art will appreciate that all or part of step realizing above-described embodiment can have been come by hardware, the hardware that also can control to be correlated with by program completes, described program can be stored in a kind of computer-readable recording medium, the above-mentioned storage medium mentioned can be ROM (read-only memory), disk or CD etc.
Claims (7)
1. an immune detection micro-fluidic chip fluorescent quantitation automatic detection device, it is characterized in that, comprise: excitation source (1), exciting light focus module (2), linear movement console module (3), phosphor collection module (5), photoelectric conversion module (6), circuit module (7), waste reaction solution removes module (8), reaction automatically performs module (9), circuit module (7) comprises master controller (71), submaster controller (72) and extraneous interface module (73), wherein reaction automatically performs model calling to submaster controller (72), the reaction one or more linear electric motors automatically performed in module drive the reaction liquid storage Micropump of micro-fluidic chip, all kinds of reaction reagent is driven into relevant conversion zone, automatically completes detection reaction,
Waste reaction solution removes module integration microheater and removes waste reaction solution by evaporation profiles, and working temperature is detected by the micro thermocouple temperature sensor being fixed on its surface and feeds back to the submaster controller in circuit module;
The original hot spot that excitation source (1) produces is line spot by the focus of cylindrical mirror in exciting light focus module (2), projects on micro-fluidic chip (4); Drive linear motion platform (3) by submaster controller (72) motion control to stepper motor, thus achieve the Scanning Detction of micro-fluidic chip (4); Fluorescent marker in the upper two-dimentional rule detection region of micro-fluidic chip (4) is excited by the linear hot spot that excites, the fluorescence signal produced arrives photoelectric conversion module (6) through phosphor collection module (5), and light signal is converted to electric signal; The photosignal of continuous acquisition is delivered to master controller (71) by submaster controller (72), and master controller (71) is extrapolated by fluorescence signal intensity distributed intelligence and obtained testing sample concentration, or number of cells.
2. immune detection micro-fluidic chip fluorescent quantitation automatic detection device according to claim 1, is characterized in that,
Exciting light focus module (2) and linear movement console module (3) between them in miter angle; Phosphor collection module (5) is mutual vertical with linear movement console module (3).
3. immune detection micro-fluidic chip fluorescent quantitation automatic detection device according to claim 1, is characterized in that,
Exciting light is converted to by the cylindrical mirror of exciting light focus module (2) line spot scanning micro-fluidic chip, excites fluorescent marker in the upper two-dimentional rule detection region of micro-fluidic chip (4).
4. the immune detection micro-fluidic chip fluorescent quantitation automatic detection device according to any one of claim 1-3, is characterized in that,
Linear movement console module (3) comprises stepper motor, driving belt and the draw-in groove fixing with driving belt, wherein draw-in groove is for placing micro-fluidic chip (4) to be detected, when stepper motor rotates under the effect of submaster controller (72), driving belt, draw-in groove and micro-fluidic chip (4) is driven to do constant velocity linear's motion.
5. the immune detection micro-fluidic chip fluorescent quantitation automatic detection device according to any one of claim 1-4, is characterized in that,
This device also comprises the high-pass filter (22) be arranged between phosphor collection module (5) and photoelectric conversion module (6), high-pass filter (22) filter wavelength is greater than the wavelength of exciting light, and the bandpass filter (21) be arranged between excitation source (1) and exciting light focus module (2), after bandpass filter (21) filters, wavelength is at excitation wavelength ± 35nm, is respectively used to entering the fluorescence of photoelectric conversion module (6) and filtering the exciting light entering exciting light focus module (2).
6. the immune detection micro-fluidic chip fluorescent quantitation automatic detection device according to any one of claim 1-4, is characterized in that,
The extraneous interface module (73) of this device comprises Bluetooth wireless communication module.
7. the immune detection micro-fluidic chip fluorescent quantitation automatic detection device according to any one of claim 1-4, is characterized in that,
This device excitation source (1) is great power LED, is driven by constant current source, and submaster controller (72) controls the on off state of excitation source (1), and digitizing arranges constant current source size to regulate the brightness of light source.
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| CN113029919B (en) * | 2021-03-13 | 2024-04-02 | 长春长光辰英生物科学仪器有限公司 | Cell enrichment and fluorescence counting detection device and detection and counting method |
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