CN103141444B - Method for quickly evaluating optimal harvest time of black solider fly larvae - Google Patents
Method for quickly evaluating optimal harvest time of black solider fly larvae Download PDFInfo
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- CN103141444B CN103141444B CN201310075531.2A CN201310075531A CN103141444B CN 103141444 B CN103141444 B CN 103141444B CN 201310075531 A CN201310075531 A CN 201310075531A CN 103141444 B CN103141444 B CN 103141444B
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Abstract
The invention discloses a method for quickly evaluating the optimal harvest time of black solider fly larvae. Cultivating black solider fly larvae under a certain cultivation condition after the black solider fly larvae grow to the fourth day; randomly selecting a plurality of larvae every day, cleaning the larvae with clean water, removing water on the body surfaces of the larvae, and weighing the larvae; repeating the operations every day until the larvae grow to a prepupa stage; comparing the weights of larvae growing for different days with those of the prepupae; and determining the optimal harvest time of the larvae under the cultivation condition when the weights of the larvae are not obviously different from those of the prepupae. Through the method, the feeding condition of the black solider fly larvae can be quickly evaluated, and the optimal harvest time of the black solider fly larvae can be determined, so as to shorten the feeding period, reduce the production cost and improve the resource utilization efficiency; and the method is suitable for popularization and application of artificial large-scale cultivation of black solider flies, thereby creating conditions for utilization of black solider flies.
Description
Technical field:
The invention belongs to insect and raise field, be specifically related to a kind of rapid evaluation black soldier flies larva raising situation, to determine the method for its best harvest time.
Background technology:
Speck flat angle stratiomyiid (Hermetia illucens), be commonly called as black soldier flies (Black soldier fly), the all one's life of black soldier flies comprises ovum phase, larval phase, pupa time and adult stage, at occurring in nature, black soldier flies larva with animal wastes, rotten organic matter for food, these organic matters can be converted into efficiently idiotrophic material (May, 1961).Black soldier flies, as a kind of important resource insect, is produced in animal feed at converting animal-poultry faeces and rubbish from cooking, is shown good application prospect (Booram et al.1977; Hale, 1973; Lin Qixun etc., 1998; Newton et al., 2005).Black soldier flies larval phase, is divided into again 6 different length of times, and the 6th instar larvae is called as prepupa, and this period no longer takes food, and climbs out of medium voluntarily and finds dry place and pupate, and what utilize this characteristic can realize polypide and medium is separated and collects (Booth, 1984; Sheppard, 2002).
Existing document all shows that black soldier flies larval phase, prepupal period and pupa time produce animal feed economic worth the highest period (Hale, 1973; Newton et al., 1977; Booram et al., 1977; Analogy state brightness etc., 2009).But it is longer that black soldier flies larvae development to the phases-time of prepupa or pupa goes through the phase, and it is larger to go through phase fluctuation.May(1961), under being reported in 27.8 DEG C of conditions, larvae development goes through phase 31d.Under 27 DEG C of conditions, Tomberlin et al., (2002) adopt three kinds of medium feeding black stratiomyiids of artificial preparation, find that its larvae development goes through phase 22-24d.Although the trophic level of development duration and medium, temperature and environmental condition closely bound up (Tomberlin and Sheppard, 2002), larval weight is not increase with the propelling of development time always.Black soldier flies mature larva takes off skin and becomes prepupa, and body weight even occurs declining (Tomberlin et al., 2002).
Therefore, in production practices, in order to shorten breeding cycle, to reduce production cost, improve the level of resources utilization.A urgently rapid evaluation black soldier flies larva raising situation, to determine the method for its best harvest time.
Summary of the invention:
The object of this invention is to provide a kind of rapid evaluation black soldier flies larva raising situation, to determine its best harvest time, thus shorten breeding cycle, reduce production cost, improve the method for the best harvest time of express delivery assessment black soldier flies larva of the level of resources utilization.
The method of the best harvest time of express delivery assessment black soldier flies larva of the present invention, is characterized in that, comprise the following steps:
After black soldier flies larvae development to 4 age in days, cultivate under certain condition of culture, day by day random choose some larvas, clean with clear water, blot body surface moisture and weigh, every day so operates, until larvae development is to the prepupa stage, difference being grown the larval weight of age in days and prepupa weight ratio comparatively, time larval weight reaches unconspicuous with prepupa weight differences, is exactly the Best Times of the results larva under this condition of culture.
If change a condition of culture, then redeterminate the Best Times of the results larva under this condition of culture according to the method described above.
Larva after general selection 4 age in days, this is because 4 age in days individualities in early stage are less, not easily observes measurement.
Some described larvas are preferably >=30 larvas.
The described body surface moisture that blots is weighed, and preferably adopts filter paper to blot body surface moisture.
Described weigh after larva preferably throw away after the weighing to be put in medium and continue to cultivate.
The present invention can rapid evaluation black soldier flies larva raising situation, determines its best harvest time, thus shortens breeding cycle, reduce production cost, improve the level of resources utilization, be suitable for applying of the artificial large-scale farming of black soldier flies, thus create condition for the utilization of black soldier flies.
Accompanying drawing illustrates:
Fig. 1 is that black soldier flies 4 day old larva of embodiment 1 is to prepupa growth course body weight gain figure (25 DEG C), note: in figure, larva is heavily the mean value repeated for three times, data point subscript same letter represents that difference is not significantly (the reliable significant difference method of Tukey) in 0.05 level.
Fig. 2 be black soldier flies 4 day old larva of embodiment 2 to prepupa growth course body weight gain figure, note: in figure, larva is heavily the mean value repeated for three times, data point subscript same letter represents that difference is not significantly (the reliable significant difference method of Tukey) in 0.05 level.
Embodiment:
Following examples further illustrate of the present invention, instead of limit the present invention.
Embodiment 1:
Black soldier flies larva medium be dregs of beans, wheat bran in mass ratio 1:1 mixing and water adding to water content 70%, under medium normal temperature spontaneous fermentation within 24 hours, to be placed on 0.2 × 0.1 × 0.1m(long × wide × high) in plastic casing.Each plastic casing is inoculated at the beginning of 300 and is hatched black soldier flies larva, then plastic casing is placed in 25 DEG C, cultivates under the climatic cabinate condition under RH70%, 12D:12L condition.After larvae development to 4d age, every day, single box extracted 50 larvas, and clear water is cleaned, then blotted body surface moisture with filter paper and weigh, and still put by larva after weighing in medium and continue to raise, every day so operates, until larvae development is to the prepupa stage.Difference being grown the larval weight in age in days stage and prepupa weight ratio comparatively, time larval weight reaches unconspicuous with prepupa weight differences, is namely the Best Times of results larva.
Black soldier flies 4 day old larva to prepupa growth course body weight gain figure as shown in Figure 1, as seen from Figure 1, black soldier flies larva 4 age in days to 8 age in days body weight obviously increases, after 9 ages in days, body weight reaches about about 0.2g, but body weight gain slows down, the prepupa stage to be developed to, prepupa loses weight, with 8 day old larva body weight without significant difference.With regard to biomass, the output that black soldier flies is cultured to 8 ages in days is with to be cultured to the output in prepupa stage suitable.Therefore, under the condition of culture of the present embodiment, treat that larvae development to 8 age in days can be gathered in the crops, without the need to treating that it is developed to the prepupa stage.Thus shorten the production cycle, reduce production cost, improve the level of resources utilization.
Embodiment 2:
Black soldier flies larva medium is that 4:1 mixing and water adding is to water content 65% in mass ratio for chicken manure, wheat bran, and medium is placed in 2 × 1.5 × 0.5m cement pit.Each cement pit is inoculated at the beginning of 8000 and is hatched black soldier flies larva, cultivates under being placed in normal temperature laboratory.After larvae development to 4d age, 100 larvas are extracted in each pond day by day, and clear water is cleaned, then blot body surface moisture with filter paper and weigh, and still put by larva after weighing in medium and continue to raise, every day so operates, until larvae development is to the prepupa stage.Difference being grown the larval weight in age in days stage and prepupa weight ratio comparatively, time larval weight reaches unconspicuous with prepupa weight differences, is namely the Best Times of results larva.
Black soldier flies 4 day old larva to prepupa growth course body weight gain figure as shown in Figure 2, as seen from Figure 2, black soldier flies larva 4 age in days to 8 age in days body weight obviously increases, body weight gain is to about 0.18g, after 9 ages in days, body weight gain slows down, the prepupa stage to be developed to, prepupa loses weight, with 8 day old larva body weight without significant difference.Therefore, in the production practices under this condition of culture, treat that larvae development to 8 age in days can be gathered in the crops, without the need to treating that it is developed to the prepupa stage.Thus shorten the production cycle, reduce production cost, improve the level of resources utilization.
Claims (4)
1. a method for the best harvest time of rapid evaluation black soldier flies larva, is characterized in that, comprise the following steps:
After black soldier flies larvae development to 4 age in days, cultivate under certain condition of culture, day by day random choose some larvas, clean with clear water, blot body surface moisture and weigh, every day so operates, until larvae development is to the prepupa stage, difference being grown the larval weight of age in days and prepupa weight ratio comparatively, time larval weight reaches unconspicuous with prepupa weight differences, is exactly the Best Times of the results larva under this condition of culture.
2. method according to claim 1, is characterized in that, some described larvas are >=30 larvas.
3. method according to claim 1, is characterized in that, described to blot that body surface moisture weighs be adopt filter paper to blot body surface moisture.
4. method according to claim 1, is characterized in that, described weigh after larva throw away after the weighing to be put in medium and continue to cultivate.
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| CN108029641A (en) * | 2018-02-07 | 2018-05-15 | 河南恩赛姆生物科技有限公司 | A kind of intelligent full-automatic black soldier flies bioreactor, production line and application method |
| CN110074007B (en) * | 2019-05-14 | 2021-08-10 | 广州无两生物科技有限公司 | Livestock and poultry manure treatment method |
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| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN101978823A (en) * | 2010-11-25 | 2011-02-23 | 广东省昆虫研究所 | Method for artificially feeding black soldier flies |
| CN102058039A (en) * | 2010-12-31 | 2011-05-18 | 广东省农业科学院农业生物技术研究所 | Method for producing insect protein feed through treating livestock and poultry manure by utilizing ptecticus aurife |
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| WO2010002188A2 (en) * | 2008-07-01 | 2010-01-07 | 그린테코 주식회사 | Method for rearing soldier flies |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN101978823A (en) * | 2010-11-25 | 2011-02-23 | 广东省昆虫研究所 | Method for artificially feeding black soldier flies |
| CN102058039A (en) * | 2010-12-31 | 2011-05-18 | 广东省农业科学院农业生物技术研究所 | Method for producing insect protein feed through treating livestock and poultry manure by utilizing ptecticus aurife |
Non-Patent Citations (2)
| Title |
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| 金黄指突水虻作为蛋白质饲料的营养价值评价;叶明强等;《中国饲料》;20120330(第3期);第40-42页 * |
| 黑水虻人工饲料含水量优化与产酶枯草芽孢杆菌研究;何国宝;《中山大学硕士学位论文》;20100601;第15页第4-7段，以及图2-1，表2-1 * |
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