CN102847477B - Magnetic stirring device and stirring method thereof - Google Patents
Magnetic stirring device and stirring method thereof Download PDFInfo
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- CN102847477B CN102847477B CN201210362001.1A CN201210362001A CN102847477B CN 102847477 B CN102847477 B CN 102847477B CN 201210362001 A CN201210362001 A CN 201210362001A CN 102847477 B CN102847477 B CN 102847477B
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Abstract
Provided are a magnetic stirring device and a stirring method thereof. The magnetic stirring device comprises two portions including coils and a power supply, the coils comprise three pairs of orthorhombic Helmholtz coils and one pair of Maxwell coils, axial directions of the three pairs of Helmholtz coils respectively opposite to an X, a Y and a Z shaft of the magnetic stirring device, shaft centers of the three pairs of Helmholtz coils cross at a coordinate original point O, XOY is a horizontal plane, and an OZ shaft is upwards vertical. Axes of the one pair of Maxwell coils coincide with the Z shaft, and a shaft center of one pair of Maxwell coils coincides with the coordinate original point O. Current in four pairs of coils is controlled to enable the four pairs of coils to generate even magnetic fields and gradient magnetic fields in different time sequences, nanometer magnetic particles are pushed to move, accordingly mixing between nanometer magnetic beads and a solvent is achieved.
Description
Technical field
The present invention relates to a kind of magnetic stirring apparatus and stirring means thereof, particularly the mixing stirring device of nanometer magnetic bead and method.
Background technology
Nanometer magnetic bead is from birth, and it just receives the concern of researcher, and in the application that biochemical analysis field is succeeded.In recent years, by nanometer magnetic bead bag by upper specific antibody, acceptor, single stranded DNA, for separating of the target body in complex sample, obtain immense success.Compared with traditional separation method, nanometer magnetic bead is used for the separation of biological sample of complex component, can realizes being separated and enrichment is carried out simultaneously, substantially increase separating rate and bioaccumulation efficiency, while also make analysis detect sensitivity greatly improve.At present, this magnetic microsphere has been widely used in multiple field such as fixing of immunoassay, separate nucleic acid extraction, cell sorting, enzyme.
An important step in nanometer magnetic field application is fully mixed with reagent by nano magnetic.Existing technology is generally that the test tube that sample is housed is realized mixing by the mode of mechanical oscillation.Test tube containing nanometer magnetic bead is placed in the middle of two pieces of magnets of rotation by patent CN201249110Y, makes the magnetic bead bottom test tube rotate the object reaching mixing with magnetic force.But this patent has the motor of mechanical movement, and control mode is single, mixed effect cannot be regulated.
Summary of the invention
The object of the invention is the shortcoming overcoming prior art, propose a kind of magnetic stirring apparatus and nanometer magnetic bead stirring means thereof.The present invention adopts a kind of automation equipment of mechanical moving component to realize fully mixing of nanometer magnetic bead and reagent, can improve mixing efficiency and reduce the workload of experimenter, and can improve the reliability of device, the service life of extension fixture.
The present invention adopts and the rotating excitation field band moving field of variable frequency and amplitude size is mixed, and concrete technical scheme is:
Field generator for magnetic of the present invention comprises coil and power supply.
Described coil comprises three pairs of Helmholtz coils and a pair Maxwell's coil totally four pairs of coils.Three pairs of Helmholtz coils are according to size orthogonal installation from small to large, and the X, Y, Z axis of the corresponding magnetic stirring apparatus of axis difference of three pairs of Helmholtz coils, three pairs of Helmholtz coil correspondences are called X-axis, Y-axis, Z axis Helmholtz coil.The axle center of three pairs of Helmholtz coils intersects at a point O, and this point is the initial point of rectangular coordinate system OXYZ.XOY is horizontal plane, and OZ axle straight up.The axis of a pair Maxwell's coil overlaps with Z axis, and the axle center of this Maxwell's coil overlaps with origin of coordinates O, is called Z axis Maxwell's coil.Described power supply is the four road current sources of band CPU, and X-axis, Y-axis, Z axis Helmholtz coil and Z axis Maxwell's coil are respectively connected a road current source.The magnetic field that Z axis Helmholtz coil produces is along OZ axle forward, and Z axis Maxwell's coil produces the gradient in magnetic field along OZ axle forward on Z axis.
Described power supply is the four road current sources of band CPU, and CPU can control direction and the size of four road current source output currents respectively.Coil described in four pairs connects a road current source respectively.
CPU of the present invention controls four road current sources and exports the electric current varied in size according to different sequential to four pairs of coils, realizes mixing of nanometer magnetic bead and reagent.Its principle and implementation method are as following:
(1) test tube that the mixed liquor of nanometer magnetic bead and reagent is housed vertically is placed on OZ axis;
(2) in Z axis Helmholtz coil, pass into electric current produce towards the magnetic field of OZ positive direction, field intensity is greater than the saturation magnetization of nanometer magnetic bead, and nanometer magnetic bead is magnetized;
(3) in Z axis Maxwell's coil, pass into the gradient magnetic that electric current produces OZ positive direction on OZ axis, gradient magnetic is enough to make magnetized nanometer magnetic bead can overcome resistance towards OZ positive movement;
(4) when most of nanometer magnetic bead moves to mixed liquor top, the electric current led in Z axis Helmholtz coil and Z axis Maxwell's coil is stopped;
(5) in X-axis Helmholtz coil and Y-axis Helmholtz coil, pass into phase difference is the rotating excitation field that the sinusoidal current of 90 degree produces in XOY plane, and the amplitude of field strength of rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, and nanometer magnetic bead is magnetized; Then in Z axis Maxwell's coil, pass into electric current produce gradient magnetic, gradient magnetic is enough to make magnetized nanometer magnetic bead can overcome resistance and produces motion;
(6) stop the electric current leading in each coil, repeat (2) ~ (5) until nanometer magnetic bead mixes with reagent.
Effect of the present invention and benefit are: realize fully mixing of nanometer magnetic bead and reagent by adding different magnetic field around test tube, whole device realizes mixing by the electric current changed in coil, method is simple, realizes different mixed effects by the amplitude of electric current in control coil and frequency.
Accompanying drawing explanation
Fig. 1 is magnetic stirring apparatus schematic diagram of the present invention;
Fig. 2 is magnetic stirring apparatus connection diagram of the present invention.
Detailed description of the invention
The present invention is further illustrated below in conjunction with the drawings and specific embodiments.
Field generator for magnetic of the present invention comprises coil 2 and power supply 1.
As shown in Figure 1, described coil 2 is three to orthogonal Helmholtz coil and a pair Maxwell's coil.Three pairs of orthogonal Helmholtz coil orthogonal nested installations from small size to large scale, the X, Y, Z axis of the corresponding magnetic stirring apparatus of axis of three pairs of Helmholtz coils.The axle center of three pairs of Helmholtz coils intersects at an O, and this point is the initial point of rectangular coordinate system OXYZ.XOY is horizontal plane, and OZ axle straight up.The axis of a pair Maxwell's coil overlaps with Z axis, and the axle center of this Maxwell's coil overlaps with origin of coordinates O.Three pairs of orthogonal Helmholtz coils are connected four road current sources respectively with a pair Maxwell's coil, and CPU controls three magnetic fields axially produced by the size controlling four road current source output currents.The Helmholtz coil of X axis is coil X, and the Helmholtz coil of Y-axis is coil Y, and the Helmholtz coil of Z-axis direction is coil Z, and the Maxwell's coil of Z-axis direction is coil MZ.
Described power supply 1 is the four road current sources of band CPU, and CPU directly can control the size of every road current source output current.
Figure 2 shows that the integrated connection schematic diagram of magnetic stirring apparatus of the present invention.Current source 1 ~ 4 connects the Helmholtz coil X of X axis, the Helmholtz coil Y of Y-axis, the Helmholtz coil Z of Z-axis direction, the Maxwell's coil MZ of Z-axis direction successively.
The method of magnetic agitation of the present invention is as follows:
(1) test tube that the mixed liquor of nanometer magnetic bead and reagent is housed vertically is placed on OZ axis;
(2) in coil Z, pass into electric current produce towards the magnetic field of OZ positive direction, field intensity is greater than the saturation magnetization of nanometer magnetic bead, and nanometer magnetic bead is magnetized;
(3) in coil MZ, pass into electric current on OZ axis, produce gradient magnetic along OZ positive direction, gradient magnetic is enough to make magnetized nanometer magnetic bead can overcome resistance towards OZ positive movement;
(4) when most of nanometer magnetic bead moves to described mixed liquor top, the electric current led in coil Z and coil MZ is stopped;
(5) passing into phase difference in coil X and coil Y is the rotating excitation field that the sinusoidal current of 90 degree produces in XOY plane, and the amplitude of field strength of rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, and nanometer magnetic bead is magnetized; Then in Z axis Maxwell's coil, pass into electric current produce gradient magnetic, gradient magnetic is enough to make magnetized nanometer magnetic bead can overcome resistance and produces motion;
(6) stop the electric current in each coil, repeat step (2) ~ step (5) until nanometer magnetic bead mixes with reagent.The frequency changing the rotating excitation field in the size and step (5) producing magnetic field in above-mentioned steps in each coil can change the mixed effect of solution.
Claims (1)
1. a magnetic agitation method, the magnetic stirring apparatus that the method uses comprises coil and power supply; Described coil is three to orthogonal Helmholtz coil and a pair Maxwell's coil totally four pairs of coils, the X, Y, Z axis of the corresponding magnetic stirring apparatus of axis difference of three pairs of described Helmholtz coils, the axle center of three pairs of Helmholtz coils meets at the initial point O of rectangular coordinate system OXYZ, XOY is horizontal plane, and OZ axle straight up; The axis of a pair described Maxwell's coil overlaps with Z axis, and the axle center of a pair described Maxwell's coil overlaps with origin of coordinates O; Described power supply is the four road current sources of band CPU, and four connect a road current source respectively to described coil,
It is characterized in that described stirring means is:
(1) test tube that the mixed liquor of nanometer magnetic bead and reagent is housed vertically is placed on OZ axis;
(2) in Z axis Helmholtz coil, pass into electric current produce towards the magnetic field of OZ positive direction, the amplitude of field strength in this magnetic field is greater than the saturation magnetization of nanometer magnetic bead, and nanometer magnetic bead is magnetized;
(3) in Z axis Maxwell's coil, pass into the gradient magnetic that electric current produces OZ positive direction on OZ axis, described gradient magnetic is enough to make magnetized nanometer magnetic bead can overcome resistance towards OZ positive movement;
(4) when most of nanometer magnetic bead moves to mixed liquor top, the electric current led in Z axis Helmholtz coil and Z axis Maxwell's coil is stopped;
(5) in X-axis Helmholtz coil and Y-axis Helmholtz coil, pass into phase difference is the rotating excitation field that the sinusoidal current of 90 degree produces in XOY plane, the amplitude of field strength of described rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, and nanometer magnetic bead is magnetized; Then in Z axis Maxwell's coil, pass into electric current produce gradient magnetic, described gradient magnetic is enough to make magnetized nanometer magnetic bead can overcome resistance and produces motion;
(6) stop the electric current in each coil, repeat step (2) ~ step (5) until nanometer magnetic bead mixes with reagent.
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| CN102847477B true CN102847477B (en) | 2015-03-25 |
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Cited By (1)
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| CN107179780A (en) * | 2017-07-06 | 2017-09-19 | 哈尔滨工业大学深圳研究生院 | A kind of visual feedback 3 D electromagnetic Micro-Robot untethered driving control system |
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| PL227303B1 (en) | 2015-04-30 | 2017-11-30 | Zachodniopomorski Univ Technologiczny W Szczecinie | Two-chamber reactor for magnetic supporting of chemical processes and the system with this reactor |
| CN107984306B (en) * | 2018-01-28 | 2023-04-14 | 吉林大学 | Magnetic field remote control vortex flow directional polishing device and polishing method |
| CN110101967A (en) * | 2019-05-31 | 2019-08-09 | 重庆科技学院 | A kind of magnetic particle magnetic control aggregation apparatus |
| CN110823875A (en) * | 2019-11-05 | 2020-02-21 | 苏州长光华医生物医学工程有限公司 | Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device |
| CN112700942A (en) * | 2020-12-16 | 2021-04-23 | 苏州大学 | Electromagnetic field platform and control system with same |
| CN113019214A (en) * | 2021-03-23 | 2021-06-25 | 苏州经贸职业技术学院 | Automatic stirring device for magnetic bead reagent and magnetic bead reagent path planning method |
| CN113398818A (en) * | 2021-06-09 | 2021-09-17 | 重庆科技学院 | Magnetorheological suspensions anti storage device that subsides based on alternating magnetic field disturbance |
| CN113658771B (en) * | 2021-08-03 | 2022-10-25 | 西安交通大学 | Composite alternating magnetic field generating device with adjustable frequency |
| CN113842853A (en) * | 2021-09-23 | 2021-12-28 | 青岛科技大学 | Magnetic control hydrate reaction generating device |
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| CN102186538A (en) * | 2008-08-25 | 2011-09-14 | 应用磁学有限责任公司 | Systems and methods for providing a magnetic resonance treatment to a subject |
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| Title |
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| Magnetic propulsion of a magnetic device using three square-Helmholtz coils and a square-Maxwell coil;Yong H. Ha et al.;《Med Biol Eng Comput》;20100107;第48卷;第139-145页 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107179780A (en) * | 2017-07-06 | 2017-09-19 | 哈尔滨工业大学深圳研究生院 | A kind of visual feedback 3 D electromagnetic Micro-Robot untethered driving control system |
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