CN102847477A - Magnetic stirring device and stirring method thereof - Google Patents
Magnetic stirring device and stirring method thereof Download PDFInfo
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- CN102847477A CN102847477A CN2012103620011A CN201210362001A CN102847477A CN 102847477 A CN102847477 A CN 102847477A CN 2012103620011 A CN2012103620011 A CN 2012103620011A CN 201210362001 A CN201210362001 A CN 201210362001A CN 102847477 A CN102847477 A CN 102847477A
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Abstract
Provided are a magnetic stirring device and a stirring method thereof. The magnetic stirring device comprises two portions including coils and a power supply, the coils comprise three pairs of orthorhombic Helmholtz coils and one pair of Maxwell coils, axial directions of the three pairs of Helmholtz coils respectively opposite to an X, a Y and a Z shaft of the magnetic stirring device, shaft centers of the three pairs of Helmholtz coils cross at a coordinate original point O, XOY is a horizontal plane, and an OZ shaft is upwards vertical. Axes of the one pair of Maxwell coils coincide with the Z shaft, and a shaft center of one pair of Maxwell coils coincides with the coordinate original point O. Current in four pairs of coils is controlled to enable the four pairs of coils to generate even magnetic fields and gradient magnetic fields in different time sequences, nanometer magnetic particles are pushed to move, accordingly mixing between nanometer magnetic beads and a solvent is achieved.
Description
Technical field
The present invention relates to a kind of magnetic stirring apparatus and stirring means thereof, particularly the mixing stirring device of nanometer magnetic bead and method.
Background technology
Nanometer magnetic bead is from being born, and it has just received the concern of researcher, and the application of succeeding at biochemical analysis field.In recent years, with the coated upper specific antibody of nanometer magnetic bead, acceptor, single stranded DNA, for separating of the target body in the complex sample, obtain immense success.Compare with traditional separation method, nanometer magnetic bead is used for the separation of the biological sample of complex component, can realize separating and enrichment is carried out simultaneously, greatly improved separating rate and bioaccumulation efficiency, the while also makes the sensitivity of analyzing and testing greatly improve.At present, this magnetic microsphere has been widely used in a plurality of fields such as fixing of immunoassay, separate nucleic acid extraction, cell sorting, enzyme.
An important step in the nanometer magnetic field application is that nano magnetic is fully mixed with reagent.The mode that existing technology generally is the test tube that will sample be housed by mechanical oscillation realizes mixing.The test tube that patent CN201249110Y will contain nanometer magnetic bead is placed in the middle of two magnets of rotation, makes the magnetic bead of test tube bottom rotate the purpose that reaches mixing with magnetic force.But this patent has the motor of mechanical movement, and control mode is single, can't regulate mixed effect.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art, propose a kind of magnetic stirring apparatus and nanometer magnetic bead stirring means thereof.The present invention adopts a kind of automation equipment realization nanometer magnetic bead of machinery-free moving component and fully mixing of reagent, can improve mixing efficiency and reduce experimenter's workload, and can improve the reliability of device, the service life of extension fixture.
The present invention adopts the rotating excitation field band moving field of variable frequency and amplitude size is mixed, and concrete technical scheme is:
Field generator for magnetic of the present invention comprises coil and power supply.
Described coil comprises three pairs of Helmholtz coils and a pair of Maxwell's coil totally four pairs of coils.The three pairs of Helmholtz coils according to size from small to large quadrature install, X, Y, the Z axis of the corresponding magnetic stirring apparatus of axial difference of three pairs of Helmholtz coils, three pairs of Helmholtz coil correspondences are called X-axis, Y-axis, Z axis Helmholtz coil.The axle center of the three pairs of Helmholtz coils O that intersects at a point, this point is the initial point of rectangular coordinate system OXYZ.XOY is horizontal plane, and the OZ axle straight up.The axis of a pair of Maxwell's coil overlaps with Z axis, and the axle center of this Maxwell's coil overlaps with origin of coordinates O, is called the Z axis Maxwell's coil.Described power supply is four road current sources with CPU, and X-axis, Y-axis, Z axis Helmholtz coil and Z axis Maxwell's coil are connected and are connected one road current source.The magnetic field that the Z axis Helmholtz coil produces is along OZ axle forward, and the Z axis Maxwell's coil produces the gradient in magnetic field along OZ axle forward at Z axis.
Described power supply is four road current sources with CPU, and CPU can control respectively direction and the size of four road current source output currents.Four pairs of described coils connect respectively one road current source.
CPU of the present invention controls the electric current that four road current sources vary in size to four pairs of coil outputs according to different sequential, and the realization nanometer magnetic bead mixes with reagent.Its principle and implementation method such as following:
The test tube that (1) mixed liquor of nanometer magnetic bead and reagent will be housed vertically is placed on the OZ axis;
(2) pass into the electric current generation towards the magnetic field of OZ positive direction in the Z axis Helmholtz coil, field intensity is greater than the saturation magnetization of nanometer magnetic bead, so that the nanometer magnetic bead magnetization;
(3) pass into electric current at the gradient magnetic of OZ axis generation OZ positive direction in the Z axis Maxwell's coil, gradient magnetic is enough to so that magnetized nanometer magnetic bead can overcome resistance towards the OZ positive movement;
(4) when most of nanometer magnetic bead moves to mixed liquor top, stop to lead to the electric current in Z axis Helmholtz coil and the Z axis Maxwell's coil;
(5) passing into phase difference in X-axis Helmholtz coil and Y-axis Helmholtz coil is that 90 sinusoidal currents of spending produce the rotating excitation field in the XOY plane, and the amplitude of field strength of rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, so that the nanometer magnetic bead magnetization; Then pass into electric current and produce gradient magnetic in the Z axis Maxwell's coil, gradient magnetic is enough to produce motion so that magnetized nanometer magnetic bead can overcome resistance;
(6) stop to lead to electric current in each coil, repeat (2)～(5) until nanometer magnetic bead and reagent mix are even.
Effect of the present invention with benefit is: realize fully mixing of nanometer magnetic bead and reagent by adding different magnetic field around the test tube, whole device is realized mixing by the electric current that changes in the coil, method is simple, can realize different mixed effects with frequency by the amplitude of electric current in the control coil.
Description of drawings
Fig. 1 is magnetic stirring apparatus schematic diagram of the present invention;
Fig. 2 is magnetic stirring apparatus connection diagram of the present invention.
The specific embodiment
Further specify the present invention below in conjunction with the drawings and specific embodiments.
Field generator for magnetic of the present invention comprises coil 2 and power supply 1.
As shown in Figure 1, described coil 2 is three pairs of quadrature Helmholtz coils and a pair of Maxwell's coil.Three pairs of quadrature Helmholtz coils are from small size to the nested installation of large scale quadrature, the X of the axial corresponding magnetic stirring apparatus of three pairs of Helmholtz coils, Y, Z axis.The axle center of three pairs of Helmholtz coils intersects at an O, and this point is the initial point of rectangular coordinate system OXYZ.XOY is horizontal plane, and the OZ axle straight up.The axis of a pair of Maxwell's coil overlaps with Z axis, and the axle center of this Maxwell's coil overlaps with origin of coordinates O.The three pairs of quadrature Helmholtz coils be connected Maxwell's coil and connect respectively four road current sources, CPU controls three magnetic fields that axially produce by the size of controlling four road current source output currents.The Helmholtz coil of X axis is coil X, and the Helmholtz coil of Y-axis is coil Y, and the Helmholtz coil of Z-axis direction is coil Z, and the Maxwell's coil of Z-axis direction is coil MZ.
Described power supply 1 is four road current sources with CPU, and CPU can directly control the size of every road current source output current.
Figure 2 shows that the integrated connection schematic diagram of magnetic stirring apparatus of the present invention.Current source 1～4 connects Helmholtz coil X, the Helmholtz coil Y of Y-axis, the Helmholtz coil Z of Z-axis direction, the Maxwell's coil MZ of Z-axis direction of X axis successively.
The method of magnetic agitation of the present invention is as follows:
The test tube that (1) mixed liquor of nanometer magnetic bead and reagent will be housed vertically is placed on the OZ axis;
(2) pass into the electric current generation towards the magnetic field of OZ positive direction in coil Z, field intensity is greater than the saturation magnetization of nanometer magnetic bead, so that the nanometer magnetic bead magnetization;
(3) pass into electric current at the gradient magnetic of OZ axis generation along the OZ positive direction in coil MZ, gradient magnetic is enough to so that magnetized nanometer magnetic bead can overcome resistance towards the OZ positive movement;
(4) when most of nanometer magnetic bead moves to described mixed liquor top, stop to lead to the electric current among coil Z and the coil MZ;
(5) pass into the interior rotating excitation field of sinusoidal current generation XOY plane that phase difference is 90 degree among coil X and the coil Y, the amplitude of field strength of rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, so that the nanometer magnetic bead magnetization; Then pass into electric current and produce gradient magnetic in the Z axis Maxwell's coil, gradient magnetic is enough to produce motion so that magnetized nanometer magnetic bead can overcome resistance;
(6) stop electric current in each coil, repeating step (2)～step (5) is until nanometer magnetic bead and reagent mix are even.The frequency that changes the size that produces magnetic field in the above-mentioned steps in each coil and the rotating excitation field in the step (5) can change the mixed effect of solution.
Claims (3)
1. a magnetic stirring apparatus is characterized in that described magnetic stirring apparatus comprises coil and power supply; Described coil is the Helmholtz coil of three pairs of quadratures and a pair of Maxwell's coil totally four pairs of coils, X, Y, the Z axis of the corresponding magnetic stirring apparatus of axial difference of described three pairs of Helmholtz coils, the axle center of three pairs of Helmholtz coils meets at the initial point O of rectangular coordinate system OXYZ, XOY is horizontal plane, and the OZ axle straight up; The axis of described a pair of Maxwell's coil overlaps with Z axis, and the axle center of described a pair of Maxwell's coil overlaps with origin of coordinates O; Described power supply is four road current sources with CPU, and four pairs of described coils connect respectively one road current source.
2. according to magnetic stirring apparatus claimed in claim 1, it is characterized in that the magnetic direction of described magnetic stirring apparatus coil generation is as follows: wherein X-axis, Y-axis Helmholtz coil produce the rotating excitation field in the horizontal plane; The Z axis Helmholtz coil produces the magnetic field of OZ axle forward; The Z axis Maxwell's coil produces the gradient magnetic of OZ forward at Z axis.
3. adopt the stirring means of claim 1 or 2 described magnetic stirring apparatuses, it is characterized in that described stirring means is:
The test tube that (1) mixed liquor of nanometer magnetic bead and reagent will be housed vertically is placed on the OZ axis;
(2) pass into the electric current generation towards the magnetic field of OZ positive direction in the Z axis Helmholtz coil, the amplitude of field strength of described rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, so that the nanometer magnetic bead magnetization;
(3) pass into electric current at the gradient magnetic of OZ axis generation OZ positive direction in the Z axis Maxwell's coil, described gradient magnetic is enough to so that magnetized nanometer magnetic bead can overcome resistance towards the OZ positive movement;
(4) when most of nanometer magnetic bead moves to mixed liquor top, stop to lead to the electric current in Z axis Helmholtz coil and the Z axis Maxwell's coil;
(5) passing into phase difference in X-axis Helmholtz coil and Y-axis Helmholtz coil is that 90 sinusoidal currents of spending produce the rotating excitation field in the XOY plane, the amplitude of field strength of described rotating excitation field is greater than the saturation magnetization of nanometer magnetic bead, so that the nanometer magnetic bead magnetization; Then pass into electric current and produce gradient magnetic in the Z axis Maxwell's coil, described gradient magnetic is enough to produce motion so that magnetized nanometer magnetic bead can overcome resistance;
(6) stop electric current in each coil, repeating step (2)～step (5) is until nanometer magnetic bead and reagent mix are even.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3088509A1 (en) | 2015-04-30 | 2016-11-02 | Zachodniopomorski Uniwersytet Technologiczny w Szczecinie | Dual-chamber reactor for magnetic assisting of chemical processes and the system of this reactor |
| CN107984306A (en) * | 2018-01-28 | 2018-05-04 | 吉林大学 | A kind of magnetic field is distant to manipulate vortex flow orientation burnishing device and polishing method |
| CN110101967A (en) * | 2019-05-31 | 2019-08-09 | 重庆科技学院 | A kind of magnetic particle magnetic control aggregation apparatus |
| CN110823875A (en) * | 2019-11-05 | 2020-02-21 | 苏州长光华医生物医学工程有限公司 | Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device |
| CN113019214A (en) * | 2021-03-23 | 2021-06-25 | 苏州经贸职业技术学院 | Automatic stirring device for magnetic bead reagent and magnetic bead reagent path planning method |
| CN113398818A (en) * | 2021-06-09 | 2021-09-17 | 重庆科技学院 | Magnetorheological suspensions anti storage device that subsides based on alternating magnetic field disturbance |
| CN113658771A (en) * | 2021-08-03 | 2021-11-16 | 西安交通大学 | Composite alternating magnetic field generating device with adjustable frequency |
| CN113842853A (en) * | 2021-09-23 | 2021-12-28 | 青岛科技大学 | Magnetic control hydrate reaction generating device |
| WO2022126701A1 (en) * | 2020-12-16 | 2022-06-23 | 苏州大学 | Electromagnetic field platform and control system having same |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107179780A (en) * | 2017-07-06 | 2017-09-19 | 哈尔滨工业大学深圳研究生院 | A kind of visual feedback 3 D electromagnetic Micro-Robot untethered driving control system |
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| CN102186538A (en) * | 2008-08-25 | 2011-09-14 | 应用磁学有限责任公司 | Systems and methods for providing a magnetic resonance treatment to a subject |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3088509A1 (en) | 2015-04-30 | 2016-11-02 | Zachodniopomorski Uniwersytet Technologiczny w Szczecinie | Dual-chamber reactor for magnetic assisting of chemical processes and the system of this reactor |
| CN107984306A (en) * | 2018-01-28 | 2018-05-04 | 吉林大学 | A kind of magnetic field is distant to manipulate vortex flow orientation burnishing device and polishing method |
| CN110101967A (en) * | 2019-05-31 | 2019-08-09 | 重庆科技学院 | A kind of magnetic particle magnetic control aggregation apparatus |
| CN110823875A (en) * | 2019-11-05 | 2020-02-21 | 苏州长光华医生物医学工程有限公司 | Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device |
| WO2022126701A1 (en) * | 2020-12-16 | 2022-06-23 | 苏州大学 | Electromagnetic field platform and control system having same |
| CN113019214A (en) * | 2021-03-23 | 2021-06-25 | 苏州经贸职业技术学院 | Automatic stirring device for magnetic bead reagent and magnetic bead reagent path planning method |
| CN113398818A (en) * | 2021-06-09 | 2021-09-17 | 重庆科技学院 | Magnetorheological suspensions anti storage device that subsides based on alternating magnetic field disturbance |
| CN113658771A (en) * | 2021-08-03 | 2021-11-16 | 西安交通大学 | Composite alternating magnetic field generating device with adjustable frequency |
| CN113842853A (en) * | 2021-09-23 | 2021-12-28 | 青岛科技大学 | Magnetic control hydrate reaction generating device |
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