CN102590500A - WFR (Weil Felix Reaction) test card - Google Patents
WFR (Weil Felix Reaction) test card Download PDFInfo
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- CN102590500A CN102590500A CN2012100116954A CN201210011695A CN102590500A CN 102590500 A CN102590500 A CN 102590500A CN 2012100116954 A CN2012100116954 A CN 2012100116954A CN 201210011695 A CN201210011695 A CN 201210011695A CN 102590500 A CN102590500 A CN 102590500A
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- coomb
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a WFR (Weil Felix Reaction) test card used for detecting typhus rapidly. The WFR test card aims to solve the disadvantage that the classical WFR test stilled adopted for clinical defection of the typhus is fussy to operate and time-consuming. The WFR test card comprises a pad, wherein the pad is provided with a sample pad with water absorbency, a glass fiber strip, a chromatography film and an absorbing pad with water absorbency in a fixing manner sequentially from left to right; the glass fiber strip is uniformly coated with colorful latex particles with marks; the right end of the sample pad is jointed to the glass fiber strip; the right end of the glass fiber strip is jointed to the left end of the chromatography film; the left end of the absorbing pad is jointed to the right end of the chromatography film; the chromatography film is provided with a test line and a control line; the control line is positioned at the right side of the test line; and the test line is formed by fixing one of proteus OX19, proteus OX2 and proteus OXK on the chromatography film. The WFR test card has the advantages of being capable of detecting the typhus rapidly and having simple and convenient detection operations as well as low production cost.
Description
Technical field
The present invention relates to technical field of immunoassay, particularly a kind of outer striking coomb's test Coomb card of fast detecting typhus that is used for.
Background technology
The Clinical detection typhus still adopts classical outer striking coomb's test Coomb at present; This test is carried out tube agglutination test with patients serum's sample respectively with the three kinds of diagnosis bacterium liquid (somatic antigen) in the outer Fei Shi reagent (comprising proteus OX19 diagnosis bacterium liquid, Proteus OX2,OX19,and OXK diagnosis bacterium liquid and proteus OXK diagnosis bacterium liquid) and is detected the corresponding antibodies in the serum; Place result of determination after 16-18 hour for 37 ℃; Observe test tube bottom coagulation sedimentation situation; The above person of agglutination titer >=1:80 is positive, reports that like fertile Da Shi of 533 healthy subjects in Datong District area and outer Fei Shi agglutination test preliminary investigation as a result (Bao Ji ties up etc., the Shanxi medical magazine; 1978 the 1st phases, 42-44) record promptly is said method.
The technical matters that this method exists: 1, complex operation: earlier three kinds of diagnosis bacterium liquid of outer Fei Shi reagent are used 10 times of dilutions of physiological saline respectively; Patient samples is pressed 1:20,1:40,1:80,1:160,1:320,1:640,1:1280 doubling dilution with physiological saline, 3 parts of parallel dilutions; Each dilutability sample that parallel dilution is good and corresponding three kinds of diagnosis bacterium liquid that diluted carry out mixed in equal amounts.2, length consuming time: the sample that mixes needs could result of determination after 16-18 hour 37 ℃ of placements.Whole test needs 20 hours consuming time.
Summary of the invention
The objective of the invention is to solve the complex operation that has the Clinical detection typhus now and still adopt classical outer striking coomb's test Coomb to exist, the defective of length consuming time; A kind of outer striking coomb's test Coomb card is provided; Its ability fast detecting typhus, detecting operation is easy, and production cost is low.
The technical solution adopted for the present invention to solve the technical problems is: a kind of outer striking coomb's test Coomb card; Comprise liner; Be fixed with sample pad, fiberglass packing, chromatographic film with water absorbing properties and absorption pad on the said liner from left to right successively with water absorbing properties; Evenly be coated with the colored latex particle of tape label on the fiberglass packing, the sample pad right-hand member overlaps on fiberglass packing, and the right-hand member of fiberglass packing overlaps on the chromatographic film left end; The left end overlap joint of absorption pad is on the chromatographic film right-hand member; Chromatographic film is provided with test wire and control line, and control line is positioned at the test wire right side, and said test wire is that a kind of being fixed on the chromatographic film in proteus OX19, Proteus OX2,OX19,and OXK and three kinds of bacterium of proteus OXK formed.
Proteus OX19, Proteus OX2,OX19,and OXK and three kinds of bacterium of proteus OXK all are the antigen as outer Fei Shi reaction.
Sample pad, absorption pad are commercially available filter paper and process among the present invention, and the preferred stronger qualitative filter paper of water absorbing properties is beneficial to detection like this, increases detection speed.Chromatographic film adopts commercially available nitrocellulose filter, and nitrocellulose filter is called the NC film again, in colloid gold test paper, is used as the supporting body of C/T line, also is immunoreactive nidus simultaneously.Nitrocellulose filter model 135s, 170s or the 180s that adopts (with the second is the definition of unit: every 4cm film, how many s the chromatography time of water is).Fiberglass packing is a kind of of fiberglass products.Spun glass is a kind of Inorganic Non-metallic Materials of excellent performance.English former by name: glassfiber.Composition is silicon dioxide, aluminium oxide, calcium oxide, boron oxide, magnesium oxide, sodium oxide molybdena etc.It is to be raw material through high temperature melting, wire drawing, doff, technology such as weave cotton cloth with glass bead or discarded glass.Form various product at last; The diameter of spun glass monofilament to twenties meters microns, is equivalent to the 1/20-1/5 of a hairline from several micron, and every bundle fiber precursor all is made up of hundreds of even thousands of monofilament; Usually as the reinforcing material in the multiple material; Electrically insulating material and heat-insulating material, circuit substrate etc. are widely used in the national economy every field.
Evenly be coated with the colored latex particle of tape label on the fiberglass packing, colored latex particle generally adopts red latex particle, and colour developing obviously like this; The method of the enterprising row labels of latex particle is the routine techniques means in this area, and the inventor does not do at this and gives unnecessary details.The mark of colored latex particle band can with the IgG in Human Serum antibodies, be antigen on the test wire, detection of the present invention is completion under the promotion of chromatography effect.After blood serum sample is added on the sample pad, arrive fiberglass packing through the chromatography effect, the IgG antibody in the blood serum sample combines to form the combination particle with the colored latex particle of tape label; Further to chromatographic film, under the chromatography effect of chromatographic film, move forward then in conjunction with particle; When containing the antibody of resistance to deformation bacillus OX19, Proteus OX2,OX19,and OXK and proteus OXK in the serum (during pathology); Combine with the antigen of corresponding test wire, present the color lines of particle, unconjugated particle can continue to move forward; The color lines that combine to appear particle with control line promptly show two color lines of test wire and control line; When not containing the antibody of resistance to deformation bacillus OX19, Proteus OX2,OX19,and OXK and proteus OXK in the serum; Particle can't combine with the antigen of corresponding test wire; Test wire does not develop the color; Unconjugated particle can continue to move forward, and combines to appear the color lines of particle with control line, promptly only shows color line of control line.
Because of rete is analysed rapid speed, the colored latex particle of tape label is removable complete chromatographic film bar in a few minutes, at once can result of determination, all develop the color like test wire and control line, and serum antibody is positive; Have only line colour developing of control line, serum antibody is negative.
The X19 of Proteus; X2, the thalline O antigen of Xk bacterial strain and rickettsia exanthematotyphi and rickettsia akamushi have common antigen, so available OX19; OX2; OXk replaces Richettsia to carry out cross agglutination as antigen and respective patient serum, and this claims outer striking test, auxiliary diagnosis rickettsiosis.Proteus OX19, Proteus OX2,OX19,and OXK and proteus OXK that the present invention uses are thalline, but not bacterium liquid, that detects like this is highly sensitive.
As preferably, the main body of said liner is a plastic mattress, and the surfaces coated of plastic mattress is covered with adhesive sticker, and sample pad, fiberglass packing, chromatographic film and absorption pad all are adhesively fixed with liner.
As preferably, the colored latex particle of said tape label is the colored latex particle that is marked with SPA.(Staphylococal Protein A SPA) is a kind of protein that separates from the aureus cell wall to staphylococcal protein A.As far back as 1940, Vevwey found in some staphylococcus aureus, to contain a kind of material, in double diffusion test, can form deposition with normal human serum.Jensen also finds similar phenomenon, and with its called after A antigen.Separated this antigen up to Lofkvist in 1963 etc., and proved that it is a kind of protein.For A is distinguished with sugar mutually, Grov is called for short SPA or albumin A with its called after staphylococcal protein A.Because some immunological characteristics of SPA, it has caused numerous immunologists' interest, and develops into a kind of extremely useful instrument on the immunology.
As preferably, said control line is SPA to be fixed on the chromatographic film form.Control line is corresponding with label.
As preferably, the colored latex particle of said tape label is the colored latex particle that is marked with mouse-anti human IgG monoclonal antibody.A kind of good marking thing.
As preferably, said control line is sheep anti-mouse igg antibody to be fixed on the chromatographic film form.Sheep anti-mouse igg antibody is claimed the sheep anti-mouse igg polyclonal antibody again.Sheep anti-mouse igg (immunoglobulin (Ig)) polyclonal antibody in the quick in-vitro diagnosis product of colloidal gold method on nitrocellulose membrane (stationary phase); With the monoclonal antibody generation specific immune response of colloid gold label and show certain color, form control line and carry out immunodiagnosis.
The invention has the beneficial effects as follows: only need patient samples be done the 1:100 dilution, directly be added in the present invention and get final product, the operation ten minutes is easy; Sample is added in the present invention, and naked eyes were the may observe testing result in 10 minutes, judges about 30 minutes consuming time of whole detection, weak point consuming time.
Description of drawings
Fig. 1 is a kind of vertical view of the present invention;
Fig. 2 is a kind of sectional exploded view of the present invention.
Among the figure: 1, sample pad, 2, fiberglass packing, 3, test wire, 4, control line, 5, chromatographic film, 6, absorption pad, 7, liner.
Embodiment
Pass through specific embodiment below, and combine accompanying drawing, technical scheme of the present invention is further specified.
Embodiment 1
A kind of outer striking coomb's test Coomb card comprises liner 7, and the main body of liner 7 is a plastic mattress; The surfaces coated of plastic mattress is covered with adhesive sticker, is adhesively fixed with sample pad 1 (filter paper, Shanghai gold mark Bioisystech Co., Ltd), fiberglass packing 2 (Ahlstrom 8964 glasses with water absorbing properties on the said liner 7 from left to right successively; The outstanding Bioisystech Co., Ltd in Shanghai, model JY-J101), chromatographic film 5 (nitrocellulose filter, Millipore company; Model 135s) and have the absorption pad 6 (filter paper, Shanghai gold mark Bioisystech Co., Ltd) of water absorbing properties, evenly be coated with red latex particle (the red polystyrene latex particle of band SPA mark on the fiberglass packing 2; The outstanding Bioisystech Co., Ltd in Shanghai, model PSR030N), the method that latex particle carries out mark is a techniques well known; Do not do at this and to give unnecessary details, sample pad 1 right-hand member overlaps on fiberglass packing 2, and the right-hand member of fiberglass packing 2 overlaps on chromatographic film 5 left ends; The left end overlap joint of absorption pad 6 is on chromatographic film 5 right-hand members; There is test wire 3 in the left side at chromatographic film 5 middle parts, and test wire 3 is that (commercially available, purchase is from the subordinate's of National Institute for Food and Drugs Control Chinese medicine bacterium preservation administrative center with proteus OX19; The production code member of buying is 49101) be fixed on the chromatographic film 5 and form; There is control line 4 on the right side at chromatographic film 5 middle parts, and control line 4 is SPA (commercially available, Zhengzhou hundred basic Bioisystech Co., Ltd) to be fixed on the chromatographic film 5 form; The fixing means of proteus OX19, SPA is techniques well known, does not do at this and gives unnecessary details.
Each component specification of present embodiment: sample pad length and width: 20mm * 4mm; Fiberglass packing length and width: 10mm * 4mm; Chromatographic film length and width: 25mm * 4mm; Absorption pad length and width: 20mm * 4mm; The liner length and width are thick: 30mm * 4mm * 1mm.
A kind of outer striking coomb's test Coomb card comprises liner 7, and the main body of liner 7 is a plastic mattress; The surfaces coated of plastic mattress is covered with adhesive sticker, is adhesively fixed with sample pad 1 (filter paper, Shanghai gold mark Bioisystech Co., Ltd), fiberglass packing 2 (Ahlstrom 8964 glasses with water absorbing properties on the said liner 7 from left to right successively; The outstanding Bioisystech Co., Ltd in Shanghai, model JY-J101), chromatographic film 5 (nitrocellulose filter, Millipore company limited; Model 180s) and have the absorption pad 6 (filter paper, Shanghai gold mark Bioisystech Co., Ltd) of water absorbing properties, evenly be coated with the red latex particle of band SPA mark on the fiberglass packing 2; Sample pad 1 right-hand member overlap joint is on fiberglass packing 2, and the right-hand member of fiberglass packing 2 overlaps on chromatographic film 5 left ends, and the left end of absorption pad 6 overlaps on chromatographic film 5 right-hand members; There is test wire 3 in the left side at chromatographic film 5 middle parts, and test wire 3 is that (commercially available, purchase is from the subordinate's of National Institute for Food and Drugs Control Chinese medicine bacterium preservation administrative center with Proteus OX2,OX19,and OXK; The production code member of buying is 49102) be fixed on the chromatographic film 5 and form; There is control line 4 on the right side at chromatographic film 5 middle parts, and control line 4 is SPA (commercially available, Zhengzhou hundred basic Bioisystech Co., Ltd) to be fixed on the chromatographic film 5 form; The fixing means of Proteus OX2,OX19,and OXK, SPA is techniques well known, does not do at this and gives unnecessary details.
Each component specification of present embodiment: sample pad length and width: 18mm * 4mm; Fiberglass packing length and width: 10mm * 4mm; Chromatographic film length and width: 25mm * 4mm; Absorption pad length and width: 18mm * 4mm; The liner length and width are thick: 30mm * 4mm * 0.8mm.
A kind of outer striking coomb's test Coomb card comprises liner 7, and the main body of liner 7 is a plastic mattress; The surfaces coated of plastic mattress is covered with adhesive sticker, is adhesively fixed with sample pad 1 (filter paper, Shanghai gold mark Bioisystech Co., Ltd), fiberglass packing 2 (Ahlstrom 8964 glasses with water absorbing properties on the said liner 7 from left to right successively; The outstanding Bioisystech Co., Ltd in Shanghai, model JY-J101), chromatographic film 5 (nitrocellulose filter, U.S. Pall company; Model Pall Vivid 170s) and have the absorption pad 6 (filter paper, Shanghai gold mark Bioisystech Co., Ltd) of water absorbing properties, it is (commercially available evenly to be coated with band mouse-anti human IgG monoclonal antibody on the fiberglass packing 2; The rich credit in Shanghai bio tech ltd) the red latex particle of mark, sample pad 1 right-hand member overlaps on fiberglass packing 2, and the right-hand member of fiberglass packing 2 overlaps on chromatographic film 5 left ends; The left end overlap joint of absorption pad 6 is on chromatographic film 5 right-hand members; There is test wire 3 in the left side at chromatographic film 5 middle parts, and test wire 3 is that (commercially available, purchase is from the subordinate's of National Institute for Food and Drugs Control Chinese medicine bacterium preservation administrative center with proteus OXK; The production code member of buying is 49103) be fixed on the chromatographic film 5 and form; There is control line 4 on the right side at chromatographic film 5 middle parts, and control line 4 is sheep anti-mouse igg antibody (commercially available, river, Shanghai Xiang bio tech ltd) to be fixed on the chromatographic film 5 form; The fixing means of proteus OXK, sheep anti-mouse igg antibody is techniques well known, does not do at this and gives unnecessary details.
Each component specification of present embodiment: sample pad length and width: 20mm * 4mm; Fiberglass packing length and width: 10mm * 4mm; Chromatographic film length and width: 25mm * 4mm; Absorption pad length and width: 20mm * 4mm; The liner length and width are thick: 30mm * 4mm * 1.2mm.
The specification that need to prove each assembly of the present invention in addition can be made corresponding adjustment according to the actual requirements, and the inventor does not do one by one at this and enumerates.
When the present invention uses, only need patient samples be done the 1:100 dilution, directly be added in the present invention and get final product, the operation ten minutes is easy; Sample is added in the present invention, and naked eyes were the may observe testing result in 10 minutes, judges, all develops the color like test wire 3 and control line 4, and serum antibody is positive; Have only 4 one line colour developings of control line, serum antibody is negative, about 30 minutes consuming time of whole detection, weak point consuming time.
Above-described embodiment is a kind of preferable scheme of the present invention, is not that the present invention is done any pro forma restriction, under the prerequisite that does not exceed the technical scheme that claim puts down in writing, also has other variant and remodeling.
Claims (6)
1. outer striking coomb's test Coomb card; It is characterized in that: comprise liner (7); Be fixed with sample pad (1), fiberglass packing (2), chromatographic film (5) with water absorbing properties and absorption pad (6) on the said liner (7) from left to right successively with water absorbing properties; Evenly be coated with the colored latex particle of tape label on the fiberglass packing (2); Sample pad (1) right-hand member overlap joint is on fiberglass packing (2); The right-hand member overlap joint of fiberglass packing (2) is on chromatographic film (5) left end, and the left end of absorption pad (6) overlaps on chromatographic film (5) right-hand member, and chromatographic film (5) is provided with test wire (3) and control line (4); Control line (4) is positioned at test wire (3) right side, and said test wire (3) is that a kind of chromatographic film (5) that is fixed in proteus OX19, Proteus OX2,OX19,and OXK and three kinds of bacterium of proteus OXK is gone up formation.
2. outer striking coomb's test Coomb card according to claim 1; It is characterized in that: the main body of said liner (7) is a plastic mattress; The surfaces coated of plastic mattress is covered with adhesive sticker, and sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) all are adhesively fixed with liner (7).
3. outer striking coomb's test Coomb card according to claim 1 and 2, it is characterized in that: the colored latex particle of said tape label is the colored latex particle that is marked with SPA.
4. outer striking coomb's test Coomb card according to claim 3 is characterized in that: said control line (4) is SPA to be fixed on chromatographic film (5) go up formation.
5. outer striking coomb's test Coomb card according to claim 1 and 2, it is characterized in that: the colored latex particle of said tape label is the colored latex particle that is marked with mouse-anti human IgG monoclonal antibody.
6. outer striking coomb's test Coomb card according to claim 5 is characterized in that: said control line (4) is sheep anti-mouse igg antibody to be fixed on chromatographic film (5) go up formation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100116954A CN102590500A (en) | 2012-01-16 | 2012-01-16 | WFR (Weil Felix Reaction) test card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100116954A CN102590500A (en) | 2012-01-16 | 2012-01-16 | WFR (Weil Felix Reaction) test card |
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| CN102590500A true CN102590500A (en) | 2012-07-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012100116954A Pending CN102590500A (en) | 2012-01-16 | 2012-01-16 | WFR (Weil Felix Reaction) test card |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103360478A (en) * | 2013-07-30 | 2013-10-23 | 中国人民解放军军事医学科学院微生物流行病研究所 | Proteins for diagnosing typhus fever, and application thereof |
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2012
- 2012-01-16 CN CN2012100116954A patent/CN102590500A/en active Pending
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103360478A (en) * | 2013-07-30 | 2013-10-23 | 中国人民解放军军事医学科学院微生物流行病研究所 | Proteins for diagnosing typhus fever, and application thereof |
| CN103360478B (en) * | 2013-07-30 | 2014-12-17 | 中国人民解放军军事医学科学院微生物流行病研究所 | Proteins for diagnosing typhus fever, and application thereof |
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Application publication date: 20120718 |