CN202854145U - Joint detection card for measles, parotitis and rubella IgG (Intravenous Gamma Globulin) antibodies - Google Patents
Joint detection card for measles, parotitis and rubella IgG (Intravenous Gamma Globulin) antibodies Download PDFInfo
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- CN202854145U CN202854145U CN 201220591951 CN201220591951U CN202854145U CN 202854145 U CN202854145 U CN 202854145U CN 201220591951 CN201220591951 CN 201220591951 CN 201220591951 U CN201220591951 U CN 201220591951U CN 202854145 U CN202854145 U CN 202854145U
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- measles
- detection line
- rubella
- mumps
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Abstract
The utility model discloses a joint detection card for measles, parotitis and rubella IgG (Intravenous Gamma Globulin) antibodies. The joint detection card comprises a liner (7), a sample pad (1), a glass fiber pad (2), a chromatography film (5) and an adsorption pad (6), wherein the sample pad (1), the glass fiber pad (2), the chromatography film (5) and the adsorption pad (6) are sequentially connected in a horizontal direction and fixed onto the liner (7); coloured latex particles with marks are sprayed on the glass fiber pad (2); and a first detecting line (31), a second detecting line (32), a third detecting line (33) and a control line (4) are placed on the chromatography film (5). By adopting the joint detection card for the for measles, parotitis and rubella IgG antibodies, the IgG antibodies for measles virus, parotitis virus and rubella virus can be jointly detected by one-through operation, thus the operation process is simplified, and the purpose of quick detecting is realized.
Description
Technical field
The utility model belongs to technical field of immunoassay, particularly the antibody combined test card of a kind of measles,mumps,rubella IgG.
Background technology
Measles, mumps and rubella are the healthy Infectious Diseases of harm humans, and prevention and the diagnosis of this three classes disease are paid much attention to always.Measles, mumps and rubella triple vaccine (MMR) have played very important effect aspect prevention, the World Health Organization (WHO) knits recommendation MMR vaccine prevention and controls this three kinds of diseases.The diagnosis of crowd's measles virus, mumps virus and rubella virus IgG antibody is for the monitoring of strengthening immune effect of vaccine and detection and the epidemiology survey of prognosis, and is significant.
At present, the diagnostic kit of the existing single measles virus IgG of reagent company production testing antibody, mumps virus IgG antibody or rubella virus IgG antibody can be diagnosed respectively three kinds of virus IgG antibodies on the market.But use the IgG antibody after this class kit detects measles, mumps and rubella triple vaccine (MMR), need to buy different kits and detect respectively, complicated operation, length consuming time expends large.Therefore, although after detecting separately measles virus IgG antibody, mumps virus IgG antibody or rubella virus IgG antibody, the analysis-by-synthesis testing result there is no impact to diagnostic result again, operating process is loaded down with trivial details, detects convenient and swift not.
The utility model content
Technical problem to be solved in the utility model provides the antibody combined test card of a kind of measles,mumps,rubella IgG.This test card gets final product joint-detection measles virus, mumps virus and rubella virus IgG antibody by the single job process, thereby has simplified operating process, has realized the purpose of fast detecting.
The utility model solves the problems of the technologies described above the technical scheme that adopts: the antibody combined test card of measles,mumps,rubella IgG comprises liner, sample pad, fiberglass packing, chromatographic film and absorption pad; Described sample pad, fiberglass packing, chromatographic film and absorption pad join with the horizontal direction order and are fixed on the described liner; And be coated with the colored latex particle of tape label on the described fiberglass packing; The first detection line, the second detection line, the 3rd detection line and control line are set on the described chromatographic film.
Described the first detection line is made of measles virus antigens, and described the second detection line is made of mumps virus antigen, and described the 3rd detection line is made of rubella virus antigen.
Described the first detection line, the second detection line, the 3rd detection line and the direction of control line along sample pad to absorption pad are arranged in order.
In the technique scheme, described sample pad and absorption pad all adopt the material with water absorbing properties to be prepared from, and can be commercially available filter paper and make, and the preferred stronger qualitative filter paper of water absorbing properties is beneficial to detection like this, increases detection speed.Described chromatographic film is commercially available nitrocellulose filter, and nitrocellulose filter claims again the NC film, is used as the supporting body of C/T line in colloid gold test paper, also is immunoreactive nidus simultaneously.Nitrocellulose filter model 135s, 170s or the definition of 180s(take second as unit adopted: every 4cm film, what seconds the chromatography time of water is).Fiberglass packing is a kind of of fiberglass products.Glass fibre is a kind of Inorganic Non-metallic Materials of excellent performance.English former Glassfiber by name, composition is silicon dioxide, aluminium oxide, calcium oxide, boron oxide, magnesium oxide, sodium oxide molybdena etc.It is through high temperature melting, wire drawing, doff, the technique such as weave cotton cloth take glass bead or discarded glass as raw material, form at last various product, the glass fiber single filament diameter is from several microns to twenties microns, the 1/20-1/50 that is equivalent to a hairline, every bundle fiber precursor all is comprised of hundreds of even thousands of monofilament, usually as reinforcing material, electrically insulating material and heat-insulating material in the multiple material, circuit substrate etc. are widely used in the national economy every field.
Evenly be coated with the colored latex particle of tape label on the described fiberglass packing, colored latex particle generally adopts red latex particle, and colour developing obviously like this; The method of the enterprising row labels of latex particle is the routine techniques means in this area, and the inventor does not do at this and gives unnecessary details.The mark of colored latex particle band can be combined by the IgG antibody in human serum, is antigen on the detection line.
As preferably, described fiberglass packing is the fiberglass packing of the colored latex particle that is coated with band staphylococcal protein A (Staphylococal Protein A, SPA) mark.Staphylococcal protein A is a kind of protein that separates from the aureus cell wall.Since SPA can with the Fc end combination of the IgG of people, mouse, rabbit etc., become a kind of extremely useful instrument on the immunology.Described control line is that SPA is fixed on the control line that forms on the chromatographic film.Described control line is corresponding with label.
As preferably, described fiberglass packing is the fiberglass packing that is coated with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody.Mouse-anti human IgG monoclonal antibody is a kind of preferably label.Described control line is that sheep anti-mouse igg antibody is fixed on the control line that forms on the chromatographic film.Sheep anti-mouse igg antibody claims again the sheep anti-mouse igg polyclonal antibody.Sheep anti-mouse igg (immunoglobulin (Ig)) polyclonal antibody in the quick antibody diagnostic products of colloidal gold method on nitrocellulose filter, with the monoclonal antibody generation specific immune response of colloid gold label and show certain color, form control line and carry out immunodiagnosis.
Detection principle of the present utility model is: detection of the present utility model is to finish under the promotion of chromatography effect.After blood serum sample is added on the sample pad, arrive fiberglass packing by the chromatography effect, IgG antibody in the blood serum sample is combined with the colored latex particle of mark and is formed in conjunction with particle, then further to chromatographic film, under the chromatography effect of chromatographic film, move forward in conjunction with particle, as containing measles virus IgG antibody in the serum, (inoculate measles when mumps virus IgG antibody and rubella virus IgG antibody, should produce this three kinds of antibody behind mumps and the rubella triple vaccine), be combined with corresponding 3 detection lines, the color lines that present particle, unconjugated particle can continue to move forward, be combined the color lines that present particle with control line, namely show two color lines of detection line and control line; When not containing this several antibody in the serum, the antigen of particle and corresponding detection line can't in conjunction with, detection line does not develop the color, and for the particle of combination can continue to move forward, is combined the color lines that present particle with control line, namely only shows color line of control line.
Because rete is analysed speed, the colored latex particle of tape label is removable complete chromatographic film bar in a few minutes, at once can sentence read result, all develop the color such as the first detection line and control line, and serum measles virus IgG antibody is positive; All develop the color such as the second detection line and control line, serum mumps virus IgG antibody is positive; All develop the color such as the 3rd detection line and control line, the serum rubella virus IgG antibody is positive.Only have line colour developing of control line, serum antibody is negative.
In sum, the beneficial effects of the utility model are:
(1) the utility model test card gets final product joint-detection measles virus, mumps virus and rubella virus IgG antibody by the single job process, thereby has simplified operating process, has realized the purpose of fast detecting; And high specificity, highly sensitive.
When (2) the utility model uses, only need sample to be checked with physiological saline 1:1 dilution, directly be added on the utility model sample pad and get final product, operate very easy; Sample is added on the utility model sample pad, and naked eyes were the Observable testing result in 10 minutes, judge, and approximately 15 minutes consuming time of whole detection, consuming time short.
Description of drawings
Fig. 1 is the vertical view of embodiment 1;
Fig. 2 is the cut-open view of embodiment 1.
The corresponding component names of mark is among the figure: 1-sample pad, 2-fiberglass packing, 31-the first detection line, 32-the second detection line, 33-the 3rd detection line, 4-control line, 5-chromatographic film, 6-absorption pad, 7-liner.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.
As illustrated in fig. 1 and 2, the antibody combined test card of measles,mumps,rubella IgG, comprise liner 7, sample pad 1(filter paper, Shanghai gold mark Bioisystech Co., Ltd), fiberglass packing 2(Ahlstrom8964 glass, the outstanding Bioisystech Co., Ltd in Shanghai, model JY-J101), chromatographic film 5(nitrocellulose filter, Millipore company, model 135s) and absorption pad 6(filter paper, Shanghai gold mark Bioisystech Co., Ltd).Sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with the horizontal direction order and are fixed on the described liner 7.
Described fiberglass packing 2 is for evenly being coated with red latex particle (the red polystyrene latex particle with the SPA mark, the outstanding Bioisystech Co., Ltd in Shanghai, model PSR030N) method that fiberglass packing, latex particle carry out mark is techniques well known, and therefore not to repeat here.
The first detection line 31, the second detection line 32, the 3rd detection line 33 and control line 4 are set on the described chromatographic film 5.Described the first detection line 31, the second detection line 32, the 3rd detection line 33 and control line 4 on chromatographic film along 6 direction is arranged in order setting from sample pad 1 to absorption pad.The first detection line 31 is that the purifying measles virus antigens with deactivation is fixed on the chromatographic film 5 and forms, the second detection line 32 is that the purifying mumps virus antigen with deactivation is fixed on the chromatographic film 5 and forms, and the 3rd detection line 33 is that the purifying rubella virus antigen with deactivation is fixed on the chromatographic film 5 and forms.
In the utility model, described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 join with the horizontal direction order.Particularly, the mode that described sample pad 1, fiberglass packing 2, chromatographic film 5 and absorption pad 6 orders are joined is partly overlapping, that is: the tail end of sample pad 1 is overlapped on the fiberglass packing 2, the tail end of fiberglass packing 2 is overlapped on the head end of chromatographic film 5, and the head end of absorption pad 6 is overlapped on the tail end of chromatographic film 5.
The present embodiment is substantially the same manner as Example 1, its different piece only is: described fiberglass packing 2 is for evenly being coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 forms for sheep anti-mouse igg antibody is fixed on the chromatographic film 5.
Embodiment 3
The present embodiment is substantially the same manner as Example 1, and its different piece only is: the mode that the order that described sample pad 1, fiberglass packing 2, chromatographic film 5 are connected with absorption pad is joined is that head and the tail connect and do not have overlapping.
The present embodiment is substantially the same manner as Example 3, its different piece only is: described fiberglass packing 2 is for being coated with the fiberglass packing with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line 4 forms for sheep anti-mouse igg antibody is fixed on the chromatographic film 5.
Above-described embodiment is a kind of better embodiment of the present utility model, is not to any pro forma restriction of the utility model, also has other variant and remodeling under the prerequisite that is no more than the technical scheme that claim puts down in writing.
Claims (5)
1. the antibody combined test card of measles,mumps,rubella IgG is characterized in that, comprises liner (7), sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6); Described sample pad (1), fiberglass packing (2), chromatographic film (5) and absorption pad (6) join with the horizontal direction order and are fixed on the described liner (7); And be coated with the colored latex particle of tape label on the described fiberglass packing (2); The first detection line (31), the second detection line (32), the 3rd detection line (33) and control line (4) are set on the described chromatographic film (5).
2. the antibody combined test card of measles,mumps,rubella IgG according to claim 1, it is characterized in that, described the first detection line (31) is made of measles virus antigens, described the second detection line (32) is made of mumps virus antigen, and described the 3rd detection line (33) is made of rubella virus antigen.
3. the antibody combined test card of measles,mumps,rubella IgG according to claim 2, it is characterized in that, described the first detection line (31), the second detection line (32), the 3rd detection line (33) and control line (4) are arranged in order along the direction of sample pad (1) to absorption pad (6).
4. the antibody combined test card of measles,mumps,rubella IgG according to claim 3, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of SPA mark, and described control line (4) is fixed on the upper control line that forms of chromatographic film (5) for SPA.
5. the antibody combined test card of measles,mumps,rubella IgG according to claim 3, it is characterized in that, described fiberglass packing (2) is for being coated with the fiberglass packing (2) with the colored latex particle of mouse-anti human IgG labeling of monoclonal antibody, and described control line (4) is fixed on the upper control line (4) that forms of chromatographic film (5) for sheep anti-mouse igg antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220591951 CN202854145U (en) | 2012-11-09 | 2012-11-09 | Joint detection card for measles, parotitis and rubella IgG (Intravenous Gamma Globulin) antibodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220591951 CN202854145U (en) | 2012-11-09 | 2012-11-09 | Joint detection card for measles, parotitis and rubella IgG (Intravenous Gamma Globulin) antibodies |
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| Publication Number | Publication Date |
|---|---|
| CN202854145U true CN202854145U (en) | 2013-04-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220591951 Expired - Lifetime CN202854145U (en) | 2012-11-09 | 2012-11-09 | Joint detection card for measles, parotitis and rubella IgG (Intravenous Gamma Globulin) antibodies |
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| CN (1) | CN202854145U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103713128A (en) * | 2014-01-01 | 2014-04-09 | 武汉翰雄生物技术有限公司 | Mumps virus enzyme-linked assay kit |
| CN116183915A (en) * | 2022-12-27 | 2023-05-30 | 深圳粒影生物科技有限公司 | Triple antibody detection test strip for feline panleukopenia virus, feline herpesvirus and feline calicivirus and application thereof |
-
2012
- 2012-11-09 CN CN 201220591951 patent/CN202854145U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103713128A (en) * | 2014-01-01 | 2014-04-09 | 武汉翰雄生物技术有限公司 | Mumps virus enzyme-linked assay kit |
| CN116183915A (en) * | 2022-12-27 | 2023-05-30 | 深圳粒影生物科技有限公司 | Triple antibody detection test strip for feline panleukopenia virus, feline herpesvirus and feline calicivirus and application thereof |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130403 |