CA3000551C - Evenement de transformation de soja shzd32-01 et son procede d'application - Google Patents

Evenement de transformation de soja shzd32-01 et son procede d'application Download PDF

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Publication number
CA3000551C
CA3000551C CA3000551A CA3000551A CA3000551C CA 3000551 C CA3000551 C CA 3000551C CA 3000551 A CA3000551 A CA 3000551A CA 3000551 A CA3000551 A CA 3000551A CA 3000551 C CA3000551 C CA 3000551C
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Prior art keywords
soybean
seq
dna
plant
shzd32
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CA3000551A
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CA3000551A1 (fr
Inventor
Yueping CAO
Peiying XIAO
Luhua BO
Feng Liu
Yunyun CUI
Linbi ZHOU
Jiajin ZHANG
Xiangyu Wu
Na Li
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne une semence et un plant de soja comprenant un événement de transformation de soja SHZD32-01 et son procédé d'identification. L'événement de transformation de soja SHZD32-01 comprend une séquence de nucléotides, telle qu'un acide nucléique tel que représenté par SEQ ID NO 9, et exprime une résistance au glyphosate. L'invention concerne également un procédé de production de soja résistant au glyphosate, et un procédé de culture de soja en luttant contre des mauvaises herbes dans un champ.
CA3000551A 2015-10-09 2016-09-30 Evenement de transformation de soja shzd32-01 et son procede d'application Active CA3000551C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510646846.7A CN105219767B (zh) 2015-10-09 2015-10-09 大豆转化事件shzd32‑01及其应用方法
CN201510646846.7 2015-10-09
PCT/CN2016/101416 WO2017059795A1 (fr) 2015-10-09 2016-09-30 Événement de transformation de soja shzd32-01 et son procédé d'application

Publications (2)

Publication Number Publication Date
CA3000551A1 CA3000551A1 (fr) 2017-04-13
CA3000551C true CA3000551C (fr) 2024-02-13

Family

ID=54989037

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3000551A Active CA3000551C (fr) 2015-10-09 2016-09-30 Evenement de transformation de soja shzd32-01 et son procede d'application

Country Status (5)

Country Link
US (1) US10604768B2 (fr)
CN (1) CN105219767B (fr)
BR (1) BR112018006984A2 (fr)
CA (1) CA3000551C (fr)
WO (1) WO2017059795A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105219767B (zh) * 2015-10-09 2018-02-13 上海交通大学 大豆转化事件shzd32‑01及其应用方法
CN106498030A (zh) * 2016-09-18 2017-03-15 浙江大学 转基因大豆zuts‑33的制备方法、检测及其应用
CA3046232A1 (fr) 2016-12-22 2018-06-28 BASF Agricultural Solutions Seed US LLC Evenement elite ee-gm4 et procedes et trousses pour identifier un tel evenement dans des echantillons biologiques
UY37549A (es) 2016-12-22 2018-06-29 Bayer Cropscience Lp Evento de élite ee-gm5 y métodos y kits para identificar tal evento en muestras biológicas
CN106636445A (zh) * 2017-03-01 2017-05-10 浙江省农业科学院 耐除草剂大豆shzd32‑1及其衍生品种的实时荧光定性pcr检测方法
CN106702006B (zh) * 2017-03-01 2020-07-17 浙江省农业科学院 耐除草剂大豆shzd32-1及其衍生品种的定性pcr检测方法
KR20210042430A (ko) * 2018-07-31 2021-04-19 콜플랜트 리미티드 담배 형질전환 이벤트 및 이의 검출 방법 및 용도
CN111663000A (zh) * 2020-07-06 2020-09-15 中国农业科学院作物科学研究所 一种大豆闭锁花分子标记及其应用
CN112359123A (zh) * 2020-10-10 2021-02-12 浙江省农业科学院 转g10evo-epsps基因大豆实时荧光定量检测方法及其试剂盒
CN112359127A (zh) * 2020-11-13 2021-02-12 浙江省农业科学院 一种用于检测转基因大豆shzd32-1的rpa引物和探针组合及现场快速检测方法
CN116144672B (zh) * 2022-09-23 2023-11-07 隆平生物技术(海南)有限公司 转基因玉米事件lp026-1及其检测方法
CN116656869B (zh) * 2023-07-24 2023-09-22 捷康生物科技(海南)有限公司 转基因大豆事件jk1001-2及其检测方法
CN116716434B (zh) * 2023-08-01 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp012-3及其检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5094945A (en) 1983-01-05 1992-03-10 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
EP0536330B1 (fr) 1990-06-25 2002-02-27 Monsanto Technology LLC Plantes tolerant le glyphosate
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US6040497A (en) 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
US6489542B1 (en) * 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
EP1322773A2 (fr) 2000-09-29 2003-07-02 Monsanto Technology LLC Plante de ble 33391 resistante au glyphosate et compositions et procedes de detection de celle-ci
AU1536302A (en) 2000-10-25 2002-05-06 Monsanto Technology Llc Cotton event pv-ghgt07(1445) and compositions and methods for detection thereof
EP1417318B1 (fr) 2000-10-30 2011-05-11 Monsanto Technology LLC Colza canola pv-bngt(rt73), compositions et procedes de detection correspondants
AP2693A (en) * 2005-05-27 2013-07-16 Monsanto Technology Llc Soybean event MON89788 and methods for detection thereof
CN105219767B (zh) * 2015-10-09 2018-02-13 上海交通大学 大豆转化事件shzd32‑01及其应用方法

Also Published As

Publication number Publication date
WO2017059795A1 (fr) 2017-04-13
CN105219767A (zh) 2016-01-06
US10604768B2 (en) 2020-03-31
CN105219767B (zh) 2018-02-13
US20180230484A1 (en) 2018-08-16
CA3000551A1 (fr) 2017-04-13
BR112018006984A2 (pt) 2018-10-23

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