ZA200603531B - Phenyl derivatives as PPAR agonists - Google Patents
Phenyl derivatives as PPAR agonists Download PDFInfo
- Publication number
- ZA200603531B ZA200603531B ZA200603531A ZA200603531A ZA200603531B ZA 200603531 B ZA200603531 B ZA 200603531B ZA 200603531 A ZA200603531 A ZA 200603531A ZA 200603531 A ZA200603531 A ZA 200603531A ZA 200603531 B ZA200603531 B ZA 200603531B
- Authority
- ZA
- South Africa
- Prior art keywords
- methyl
- phenyl
- alkyl
- trifluoromethyl
- phenoxy
- Prior art date
Links
- 239000000556 agonist Substances 0.000 title claims description 15
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 title claims description 15
- 102000003728 Peroxisome Proliferator-Activated Receptors Human genes 0.000 title claims description 14
- 108090000029 Peroxisome Proliferator-Activated Receptors Proteins 0.000 title claims description 14
- 150000001875 compounds Chemical class 0.000 claims description 342
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 124
- XBDQKXXYIPTUBI-UHFFFAOYSA-N Propionic acid Substances CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 77
- 229910052739 hydrogen Inorganic materials 0.000 claims description 56
- 239000001257 hydrogen Substances 0.000 claims description 55
- 238000000034 method Methods 0.000 claims description 53
- 238000011282 treatment Methods 0.000 claims description 38
- 150000002148 esters Chemical class 0.000 claims description 35
- 239000000203 mixture Substances 0.000 claims description 32
- 150000002431 hydrogen Chemical class 0.000 claims description 28
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 26
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 22
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 22
- 201000010099 disease Diseases 0.000 claims description 21
- 229910052736 halogen Inorganic materials 0.000 claims description 16
- 150000002367 halogens Chemical class 0.000 claims description 16
- 150000003839 salts Chemical class 0.000 claims description 16
- 208000001072 type 2 diabetes mellitus Diseases 0.000 claims description 16
- 125000000217 alkyl group Chemical group 0.000 claims description 15
- 125000003118 aryl group Chemical group 0.000 claims description 13
- 229910052760 oxygen Inorganic materials 0.000 claims description 13
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 12
- 206010012601 diabetes mellitus Diseases 0.000 claims description 12
- 108010023302 HDL Cholesterol Proteins 0.000 claims description 11
- 125000004093 cyano group Chemical group *C#N 0.000 claims description 11
- 208000032928 Dyslipidaemia Diseases 0.000 claims description 10
- 208000017170 Lipid metabolism disease Diseases 0.000 claims description 10
- 108010010234 HDL Lipoproteins Proteins 0.000 claims description 9
- 238000008214 LDL Cholesterol Methods 0.000 claims description 9
- 208000001145 Metabolic Syndrome Diseases 0.000 claims description 9
- 101150014691 PPARA gene Proteins 0.000 claims description 9
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 claims description 9
- 150000002632 lipids Chemical class 0.000 claims description 9
- 208000011580 syndromic disease Diseases 0.000 claims description 9
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 9
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- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 claims description 7
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- 125000001072 heteroaryl group Chemical group 0.000 claims description 7
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- UQJUTRMJLJUAHX-UHFFFAOYSA-N 2-[4-[[4-(methoxymethyl)-2-[4-(trifluoromethoxy)phenyl]pyrimidin-5-yl]methoxy]-2-methylphenoxy]-2-methylpropanoic acid Chemical compound COCC1=NC(C=2C=CC(OC(F)(F)F)=CC=2)=NC=C1COC1=CC=C(OC(C)(C)C(O)=O)C(C)=C1 UQJUTRMJLJUAHX-UHFFFAOYSA-N 0.000 claims description 6
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- KHKLYTPBOIGMAX-UHFFFAOYSA-N 2-[2-(3-methoxypropyl)-4-[methyl-[[2-methyl-6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]methyl]amino]phenoxy]acetic acid Chemical compound C1=C(OCC(O)=O)C(CCCOC)=CC(N(C)CC=2C(=NC(=CC=2)C=2C=CC(=CC=2)C(F)(F)F)C)=C1 KHKLYTPBOIGMAX-UHFFFAOYSA-N 0.000 claims description 4
- MSAHMFUJOUJKDS-UHFFFAOYSA-N 2-methyl-2-[2-methyl-4-[[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]methoxy]phenoxy]propanoic acid Chemical compound C1=C(OC(C)(C)C(O)=O)C(C)=CC(OCC=2C=NC(=CC=2)C=2C=CC(OC(F)(F)F)=CC=2)=C1 MSAHMFUJOUJKDS-UHFFFAOYSA-N 0.000 claims description 4
- 239000013543 active substance Substances 0.000 claims description 4
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- IHVDKBRTMGDSKV-UHFFFAOYSA-N 2-[4-[[4-(2-methoxyethyl)-6-methyl-2-[4-(trifluoromethyl)phenyl]pyrimidin-5-yl]methoxy]-2-methylphenoxy]-2-methylpropanoic acid Chemical compound COCCC1=NC(C=2C=CC(=CC=2)C(F)(F)F)=NC(C)=C1COC1=CC=C(OC(C)(C)C(O)=O)C(C)=C1 IHVDKBRTMGDSKV-UHFFFAOYSA-N 0.000 claims description 3
- PVBOHUITAXRRTB-UHFFFAOYSA-N 2-[4-[[4-(methoxymethyl)-6-methyl-2-[3-(trifluoromethyl)phenyl]pyrimidin-5-yl]methoxy]-2-methylphenoxy]-2-methylpropanoic acid Chemical compound COCC1=NC(C=2C=C(C=CC=2)C(F)(F)F)=NC(C)=C1COC1=CC=C(OC(C)(C)C(O)=O)C(C)=C1 PVBOHUITAXRRTB-UHFFFAOYSA-N 0.000 claims description 3
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- HQUOJQVGWVNXHN-UHFFFAOYSA-N 2-methyl-2-[4-[2-[[2-methyl-6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]amino]-2-oxoethyl]phenoxy]propanoic acid Chemical compound CC1=NC(C=2C=CC(=CC=2)C(F)(F)F)=CC=C1NC(=O)CC1=CC=C(OC(C)(C)C(O)=O)C=C1 HQUOJQVGWVNXHN-UHFFFAOYSA-N 0.000 claims description 3
- BLUJIOXHKNLKNT-UHFFFAOYSA-N 2-methyl-2-[4-[[[4-(trifluoromethyl)-2-[4-(trifluoromethyl)phenyl]pyrimidine-5-carbonyl]amino]methyl]phenoxy]propanoic acid Chemical compound C1=CC(OC(C)(C)C(O)=O)=CC=C1CNC(=O)C1=CN=C(C=2C=CC(=CC=2)C(F)(F)F)N=C1C(F)(F)F BLUJIOXHKNLKNT-UHFFFAOYSA-N 0.000 claims description 3
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- VUIPFWKMNDLDJE-UHFFFAOYSA-N 2-[4-[2-[[4-cyclopropyl-2-[4-(trifluoromethyl)phenyl]pyrimidin-5-yl]amino]-2-oxoethyl]phenoxy]-2-methylpropanoic acid Chemical compound C1=CC(OC(C)(C)C(O)=O)=CC=C1CC(=O)NC1=CN=C(C=2C=CC(=CC=2)C(F)(F)F)N=C1C1CC1 VUIPFWKMNDLDJE-UHFFFAOYSA-N 0.000 claims description 2
- JPAGWSQMGNTNFO-UHFFFAOYSA-N 2-[4-[[4-cyclopropyl-2-[3-(trifluoromethyl)phenyl]pyrimidin-5-yl]methoxy]-2-methylphenoxy]-2-methylpropanoic acid Chemical compound C1=C(OC(C)(C)C(O)=O)C(C)=CC(OCC=2C(=NC(=NC=2)C=2C=C(C=CC=2)C(F)(F)F)C2CC2)=C1 JPAGWSQMGNTNFO-UHFFFAOYSA-N 0.000 claims description 2
- NFRYDXDIITWMPP-UHFFFAOYSA-N 2-methyl-2-[2-methyl-4-[[2-methyl-6-[3-(trifluoromethyl)phenyl]pyridin-3-yl]methoxy]phenoxy]propanoic acid Chemical compound C1=C(OC(C)(C)C(O)=O)C(C)=CC(OCC=2C(=NC(=CC=2)C=2C=C(C=CC=2)C(F)(F)F)C)=C1 NFRYDXDIITWMPP-UHFFFAOYSA-N 0.000 claims description 2
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- QMTVRLUBUQSFKU-UHFFFAOYSA-N ethyl 2-[4-[[2-cyclopropyl-6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]methoxy]-2-methylphenoxy]-2-methylpropanoate Chemical compound C1=C(C)C(OC(C)(C)C(=O)OCC)=CC=C1OCC1=CC=C(C=2C=CC(=CC=2)C(F)(F)F)N=C1C1CC1 QMTVRLUBUQSFKU-UHFFFAOYSA-N 0.000 description 1
- XTXCRUGSIVOUCC-UHFFFAOYSA-N ethyl 2-[4-[[4-cyclopropyl-2-[4-(trifluoromethyl)phenyl]pyrimidin-5-yl]methoxy]-2-methylphenoxy]-2-methylpropanoate Chemical compound C1=C(C)C(OC(C)(C)C(=O)OCC)=CC=C1OCC1=CN=C(C=2C=CC(=CC=2)C(F)(F)F)N=C1C1CC1 XTXCRUGSIVOUCC-UHFFFAOYSA-N 0.000 description 1
- TXCFBNGSBHQCLC-UHFFFAOYSA-N ethyl 2-[4-[[6-[3-fluoro-4-(trifluoromethyl)phenyl]-2-methylpyridin-3-yl]methoxy]-2-methylphenoxy]-2-methylpropanoate Chemical compound C1=C(C)C(OC(C)(C)C(=O)OCC)=CC=C1OCC1=CC=C(C=2C=C(F)C(=CC=2)C(F)(F)F)N=C1C TXCFBNGSBHQCLC-UHFFFAOYSA-N 0.000 description 1
- PTBIZTSXEHCFQP-UHFFFAOYSA-N ethyl 2-methyl-2-[2-methyl-4-[[2-methyl-6-[3-(trifluoromethyl)phenyl]pyridin-3-yl]methoxy]phenoxy]propanoate Chemical compound C1=C(C)C(OC(C)(C)C(=O)OCC)=CC=C1OCC1=CC=C(C=2C=C(C=CC=2)C(F)(F)F)N=C1C PTBIZTSXEHCFQP-UHFFFAOYSA-N 0.000 description 1
- CYKMLXOVMZFWJX-UHFFFAOYSA-N ethyl 2-methyl-2-[2-methyl-4-[[2-methyl-6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]methoxy]phenoxy]propanoate Chemical compound C1=C(C)C(OC(C)(C)C(=O)OCC)=CC=C1OCC1=CC=C(C=2C=CC(=CC=2)C(F)(F)F)N=C1C CYKMLXOVMZFWJX-UHFFFAOYSA-N 0.000 description 1
- QRZMNONKFYZQSK-UHFFFAOYSA-N ethyl 2-methyl-2-[3-(methylamino)phenoxy]propanoate Chemical compound CCOC(=O)C(C)(C)OC1=CC=CC(NC)=C1 QRZMNONKFYZQSK-UHFFFAOYSA-N 0.000 description 1
- KDEPIQSYANNQRR-UHFFFAOYSA-N ethyl 2-methyl-2-[3-[methyl-[2-[2-methyl-6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]acetyl]amino]phenoxy]propanoate Chemical compound CCOC(=O)C(C)(C)OC1=CC=CC(N(C)C(=O)CC=2C(=NC(=CC=2)C=2C=CC(=CC=2)C(F)(F)F)C)=C1 KDEPIQSYANNQRR-UHFFFAOYSA-N 0.000 description 1
- SJAOFPHBAYOIAX-UHFFFAOYSA-N ethyl 3-amino-3-cyclopropylprop-2-enoate Chemical compound CCOC(=O)C=C(N)C1CC1 SJAOFPHBAYOIAX-UHFFFAOYSA-N 0.000 description 1
- LFSVADABIDBSBV-UHFFFAOYSA-N ethyl 3-cyclopropyl-3-oxopropanoate Chemical compound CCOC(=O)CC(=O)C1CC1 LFSVADABIDBSBV-UHFFFAOYSA-N 0.000 description 1
- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 125000000031 ethylamino group Chemical group [H]C([H])([H])C([H])([H])N([H])[*] 0.000 description 1
- 125000004705 ethylthio group Chemical group C(C)S* 0.000 description 1
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 229940125753 fibrate Drugs 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 125000005519 fluorenylmethyloxycarbonyl group Chemical group 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 125000004428 fluoroalkoxy group Chemical group 0.000 description 1
- 125000003709 fluoroalkyl group Chemical group 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 150000004795 grignard reagents Chemical class 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 231100000304 hepatotoxicity Toxicity 0.000 description 1
- 125000003187 heptyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- FFUAGWLWBBFQJT-UHFFFAOYSA-N hexamethyldisilazane Chemical compound C[Si](C)(C)N[Si](C)(C)C FFUAGWLWBBFQJT-UHFFFAOYSA-N 0.000 description 1
- 125000003707 hexyloxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 125000005027 hydroxyaryl group Chemical group 0.000 description 1
- 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 125000002883 imidazolyl group Chemical group 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 125000003387 indolinyl group Chemical group N1(CCC2=CC=CC=C12)* 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000012442 inert solvent Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000003978 infusion fluid Substances 0.000 description 1
- 229960004903 invert sugar Drugs 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- KQNPFQTWMSNSAP-UHFFFAOYSA-N isobutyric acid Chemical compound CC(C)C(O)=O KQNPFQTWMSNSAP-UHFFFAOYSA-N 0.000 description 1
- 125000000555 isopropenyl group Chemical group [H]\C([H])=C(\*)C([H])([H])[H] 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 125000000842 isoxazolyl group Chemical group 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 150000007517 lewis acids Chemical class 0.000 description 1
- 230000004322 lipid homeostasis Effects 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 206010024627 liposarcoma Diseases 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- DLEDOFVPSDKWEF-UHFFFAOYSA-N lithium butane Chemical compound [Li+].CCC[CH2-] DLEDOFVPSDKWEF-UHFFFAOYSA-N 0.000 description 1
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- 230000007056 liver toxicity Effects 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
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- UGVPKMAWLOMPRS-UHFFFAOYSA-M magnesium;propane;bromide Chemical compound [Mg+2].[Br-].CC[CH2-] UGVPKMAWLOMPRS-UHFFFAOYSA-M 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
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- XZWYZXLIPXDOLR-UHFFFAOYSA-N metformin Chemical compound CN(C)C(=N)NC(N)=N XZWYZXLIPXDOLR-UHFFFAOYSA-N 0.000 description 1
- 229960003105 metformin Drugs 0.000 description 1
- AFVFQIVMOAPDHO-UHFFFAOYSA-M methanesulfonate group Chemical class CS(=O)(=O)[O-] AFVFQIVMOAPDHO-UHFFFAOYSA-M 0.000 description 1
- QARBMVPHQWIHKH-UHFFFAOYSA-N methanesulfonyl chloride Chemical compound CS(Cl)(=O)=O QARBMVPHQWIHKH-UHFFFAOYSA-N 0.000 description 1
- 125000001160 methoxycarbonyl group Chemical group [H]C([H])([H])OC(*)=O 0.000 description 1
- SJFNDMHZXCUXSA-UHFFFAOYSA-M methoxymethyl(triphenyl)phosphanium;chloride Chemical compound [Cl-].C=1C=CC=CC=1[P+](C=1C=CC=CC=1)(COC)C1=CC=CC=C1 SJFNDMHZXCUXSA-UHFFFAOYSA-M 0.000 description 1
- XIJCNCZFLOMHIQ-UHFFFAOYSA-N methyl 2-[4-(methylamino)-2-(trifluoromethyl)phenoxy]acetate Chemical compound CNC1=CC=C(OCC(=O)OC)C(C(F)(F)F)=C1 XIJCNCZFLOMHIQ-UHFFFAOYSA-N 0.000 description 1
- NDXIPICVMAHKMI-UHFFFAOYSA-N methyl 2-[4-[[4-cyclopropyl-2-[4-(trifluoromethyl)phenyl]pyrimidin-5-yl]methyl-methylamino]-2-methylphenoxy]acetate Chemical compound C1=C(C)C(OCC(=O)OC)=CC=C1N(C)CC1=CN=C(C=2C=CC(=CC=2)C(F)(F)F)N=C1C1CC1 NDXIPICVMAHKMI-UHFFFAOYSA-N 0.000 description 1
- YDCHPLOFQATIDS-UHFFFAOYSA-N methyl 2-bromoacetate Chemical compound COC(=O)CBr YDCHPLOFQATIDS-UHFFFAOYSA-N 0.000 description 1
- RIJWDPRXCXJDPK-UHFFFAOYSA-N methyl 3-cyclopropyl-3-oxopropanoate Chemical compound COC(=O)CC(=O)C1CC1 RIJWDPRXCXJDPK-UHFFFAOYSA-N 0.000 description 1
- CVVMLRFXZPKILB-UHFFFAOYSA-N methyl 5-chloropyrazine-2-carboxylate Chemical compound COC(=O)C1=CN=C(Cl)C=N1 CVVMLRFXZPKILB-UHFFFAOYSA-N 0.000 description 1
- FPKXYXKLOWAIOX-UHFFFAOYSA-N methyl 6-chloropyridazine-3-carboxylate Chemical compound COC(=O)C1=CC=C(Cl)N=N1 FPKXYXKLOWAIOX-UHFFFAOYSA-N 0.000 description 1
- OIRDBPQYVWXNSJ-UHFFFAOYSA-N methyl trifluoromethansulfonate Chemical compound COS(=O)(=O)C(F)(F)F OIRDBPQYVWXNSJ-UHFFFAOYSA-N 0.000 description 1
- 125000000250 methylamino group Chemical group [H]N(*)C([H])([H])[H] 0.000 description 1
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- 150000007522 mineralic acids Chemical class 0.000 description 1
- 235000013536 miso Nutrition 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- PSHKMPUSSFXUIA-UHFFFAOYSA-N n,n-dimethylpyridin-2-amine Chemical compound CN(C)C1=CC=CC=N1 PSHKMPUSSFXUIA-UHFFFAOYSA-N 0.000 description 1
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
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- 235000001968 nicotinic acid Nutrition 0.000 description 1
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- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
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- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 238000005580 one pot reaction Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000010502 orange oil Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
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- 125000001181 organosilyl group Chemical group [SiH3]* 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 125000002971 oxazolyl group Chemical group 0.000 description 1
- 150000002923 oximes Chemical class 0.000 description 1
- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
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- 230000002093 peripheral effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- XMGMFRIEKMMMSU-UHFFFAOYSA-N phenylmethylbenzene Chemical group C=1C=CC=CC=1[C]C1=CC=CC=C1 XMGMFRIEKMMMSU-UHFFFAOYSA-N 0.000 description 1
- 125000003356 phenylsulfanyl group Chemical group [*]SC1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
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- 125000001501 propionyl group Chemical group O=C([*])C([H])([H])C([H])([H])[H] 0.000 description 1
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- 125000000168 pyrrolyl group Chemical group 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
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- 229960004586 rosiglitazone Drugs 0.000 description 1
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- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
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- 125000002827 triflate group Chemical group FC(S(=O)(=O)O*)(F)F 0.000 description 1
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- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical class CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 1
- 125000000026 trimethylsilyl group Chemical group [H]C([H])([H])[Si]([*])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- GXPHKUHSUJUWKP-UHFFFAOYSA-N troglitazone Chemical compound C1CC=2C(C)=C(O)C(C)=C(C)C=2OC1(C)COC(C=C1)=CC=C1CC1SC(=O)NC1=O GXPHKUHSUJUWKP-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
Phenyl derivatives as PPAR agonists
The present invention is concerned with novel phenyl derivatives of the formula
R® 6
R? R 0 xt Rr I 1 oA,
RY R’ and enantiomers and pharmaceutically acceptable salts and esters thereof, wherein
X!'is 0, S, CH
R! is hydrogen or Cy.7-alkyl;
R? is hydrogen or Cy.7-alkyl, or, if X* is CHj, R? is hydrogen, Cy.7~alkyl or Cy.7-alkoxy;
R?is hydrogen or C;.7-alkyl;
R*and R® independently from each other are hydrogen, C,.7-alkyl,
Cs.7-cycloalkyl, halogen, C,.7-alkoxy- C,.7-alkyl, C; 7-alkenyl, C;7-alkinyl, fluoro-Cy.7-alkyl, cyano-C,.7-alkyl or cyano;
RS, RS and R’ independently from each other are hydrogen, C,.;-alkyl,
Ca.7-cycloalkyl, halogen, C,.7-alkoxy- C,.7-alkyl, Cy 7-alkenyl, C,.7-alkinyl, fluoro-C,.7-alkyl, cyano-C,.7-alkyl or cyano; and one of R%, R% and R is
Y! ed < rR
IN (CH,), N\ VY? (CR1RM) ~~ wherein
X?is S, O, NK’, (CH)sNR°CO, or (CH;)pCONR’, rR’ is hydrogen, C,.7-alkyl, Cs.7-cydloalkyl, fluoro-C,7-alkyl, hydroxy-C,.7-alkyl, or
C,.7-alkoxy-Cps-alkyl;
YY: Y and Y* are Nor C-R?and 1 or 2 of Y\, Y2 Y? and Y* are N and the other ones are C-R'%
Ris C, -alkyl, Cs.-cycloalkyl, fluoro-Cy.7-alkyl, or C,.7-alkoxy-Cy.7-alkyl;
RY is hydrogen, C,7-alkyl, C..7-alkoxy-C;.7-alkyl;
R'2independently from each other in each occurance is selected from hydrogen,
C7-alkyl, Cs.s-cydloalkyl, fluoro-C;.7-alkyl, C,.7-alkoxy-Ci.7-alkyl, hydroxy-C,.7-alkyl, Cir alkylthio-C, 7-alkyl, carboxy-C, 7-alkoxy-Ci7-alkyl, carboxy, carboxy-C.7-alkyl, mono- or di-C,.;-alkyl-amino-C, 7-alkyl,
C,.7-alkanoyl-C, 7-alkyl, C,.;-alkenyl, and C,.7-alkinyl;
R"? is aryl or heteroaryl; misOorl,nis0,1,20r3,andpis0, 1 or 2, and thesum of m,nand pis 1,2,3 or 4; provided that compounds of formula I are excluded, wherein
X! is O, R? and R? are hydrogen,
R® is equal to vy! . pe
IN _ (CH), NS ve-Y* (CR10RM),_
X%is O or S,and mis 0.
Compounds such as {5-methoxy-2-methyl-4-[6-(4-trifluoromethyl-phenyl)- pyridin-3-ylmethyl-sulfanyl] -phenoxy}-acetic acid have been described in PCT patent application WO 03/084916.
Compounds of formula I are useful as lipid modulators and insulin sensitizers. In particular, compounds of formula I are PPAR activators.
Peroxisome Proliferator Activated Receptors (PPARs) are members of the nuclear hormone receptor superfamily. The PPARs are ligand-activated transcription factors that regulate gene expression and control multiple metabolic pathways. Three subtypes have been described which are PPARa, PPARS (also known as PPARB), and PPARy. PPARS is ubiquitously expressed. PPARa is predominantly expressed in the liver, kidney and heart.
There are at least two major isoforms of PPARy. PPARy1 is expressed in most tissues, and the longer isoform, PPARY2 is almost exclusively expressed in adipose tissue. The PPARs modulate a variety of physiological responses including regulation of glucose- and lipid- homeostasis and metabolism, energy balance, cell differentiation, inflammation and cardiovascular events.
Approximately half of all patients with coronary artery disease have low concentrations of plasma HDL cholesterol, The atheroprotective function of HDL was first highlighted almost 25 years ago and stimulated exploration of the genetic and environmental factors that influence HDL levels. The protective function of HDL comes from its role in a process termed reverse cholesterol transport. HDL mediates the removal of cholesterol from cells in peripheral tissues including those in the atherosclerotic lesions of the arterial wall. HDL then delivers its cholesterol to the liver and sterol-metabolizing organs for conversion to bile and elimination. Data from the
Framingham study showed that HDL-C levels are predictive of coronary artery disease risk independently of LDL-C levels. The estimated age-adjusted prevalence among
Americans age 20 and older who have HDL-C of less than 35 mg/dl is 16% (males) and 5.7% (females). A substantial increase of HDL-C is currently achieved by treatment with niacin in various formulations. However, the substantial side-effects limit the therapeutic potential of this approach.
As many as 90% of the 14 million diagnosed type 2 diabetic patients in the US are overweight or obese, and a high proportion of type 2 diabetic patients have abnormal concentrations of lipoproteins. The prevalence of total cholesterol > 240 mg/dl is 37% in diabetic men and 44% in women. The respective rates for LDL-C > 160 mg/dl are 31% and 44%, respectively, and for HDL-C < 35 mg/dl 28% and 11%, respectively. Diabetes is a disease in which a patient’s ability to control glucose levels in blood is decreased because of partial impairment in response to the action of insulin. Type II diabetes (T2D) is.also called non-insulin dependent diabetes mellitus (NIDDM) and afflicts 80-90 % of all diabetic patients in developed countries. In T2D, the pancreatic Islets of Langerhans - continue to produce insulin. However, the target organs for insulin action, mainly muscle, liver and adipose tissue, exhibit a profound resistance to insulin stimulation. The body continues to compensate by producing unphysiologically high levels of insulin, which ultimately decreases in later stage of disease, due to exhaustion and failure of pancreatic insulin-producing capacity. Thus T2D is 2 cardiovascular-metabolic syndrome associated with multiple comorbidities including insulin resistance, dyslipidemia, hypertension, endothelial dysfunction and inflammatory atherosclerosis.
Eirst line treatment for dyslipidemia and diabetes generally involves a low-fat and low-glucose diet, exercise and weight loss. However, compliance can be moderate, and as the disease progresses, treatment of the various metabolic deficiencies becomes necessary with e.g. lipid-modulating agents such as statins and fibrates for dyslipidemia and hypoglycemic drugs, e.g. sulfonylureas or metformin for insulin resistance. A promising new class of drugs has recently been introduced that resensitizes patients to their own insulin (insulin sensitizers), thereby restoring blood glucose and triglyceride levels to normal, and in many cases, obviating or reducing the requirement for exogenous insulin.
Pioglitazone (Actos™) and rosiglitazone (Avandia™) belong to the thiazolidinedione (TZD) class of PPARy-agonists and were the first in their class to be approved for
NIDDM in several countries. These compounds, however, suffer from side effects, including rare but severe liver toxicity (as seen with troglitazone). They also increase body weight in patients. Therefore, new, more efficacious drugs with greater safety and
Jower side effects are urgently needed. Recent studies provide evidence that agonism of
PPARS would result in compounds with enhanced therapeutic potential, i. e. such compounds should improve the lipid profile, with a superior effect on HDL-C raising compared to current treatments and with additional positive effects on normalization of insulin-levels (Oliver et al; Proc Nat Acad Sci USA 2001; 98: 5306-11). Recent observations also suggest that there is a independent PPARa mediated effect on insulin- sensitzation in addition to its well known role in reducing triglycerides (Guerre-Millo et al; J Biol Chem 2000; 275: 16638-16642). Thus selective PPARS agonists or PPARS agonists with additional PPAR« activity may show superior therapeutic efficacy without the side-effects such as the weight gain seen with PPARy agonists.
The novel compounds of the present invention exceed the compounds known in the art, inasmuch as they bind to and selectively activate PPARS or coactivate PPARS and
PPAR. simultaneously and very efficiently, and with much improved pharmacokinetic properties. Therefore, these compounds combine the anti-dyslipidemic and anti- glycemic effects of PPAR3 and PPAR activation with no effect on PPARy.
Consequently, HDL cholesterol is increased, triglycerides lowered (= improved lipid profile) and plasma glucose and insulin are reduced (= insulin sensitization). In addition, such compounds may also lower LDL cholesterol, decrease blood pressure and counteract inflammatory atherosclerosis. Furthermore, such compounds may also be useful for treating inflammatory diseases such as rheumatoid arthritis, osteoarthritis, and psoriasis. Since multiple facets of combined dyslipidemia and the T2D disease syndrome are addressed by PPARS—selective agonists and PPARS and a coagonists, they are expected to have an enhanced therapeutic potential compared to the compounds already known in the art.
Object of the present invention therefore is to provide compounds which must have the criteria mentioned above. Furthermore, the compounds of the present invention exhibit improved pharmacological properties compared to known compounds.
Unless otherwise indicated the following definitions are set forth to illustrate and define the meaning and scope of the various terms used to describe the invention herein.
The term “alkyl”, alone or in combination with other groups, refers to a branched or straight-chain monovalent saturated aliphatic hydrocarbon radical of one to twenty carbon atoms, preferably one to sixteen carbon atoms, more preferably one to ten carbon atoms.
The term “lower alkyl” or “Cy.7-alkyl”, alone or in combination with other groups, refers to a branched or straight-chain monovalent alkyl radical of one to seven carbon atoms, preferably one to four carbon atoms. This term is further exemplified by such radicals as methyl, ethyl, n-propyl, isopropyl, n-butyl, s-butyl, t-butyl and the groups specifically exemplified herein.
The term “halogen” refers to fluorine, chlorine, bromine and iodine.
The term “fluoro-lower alkyl” or “fluoro-C,.7-alkyl” refers to to lower alkyl groups which are mono- or multiply substituted with fluorine. Examples of fluoro-lower alkyl groups are e.g. -CFs, -CH,CF3, -CH(CF:); and the groups specifically exemplified herein.
The term “alkoxy” refers to the group R’-O-, wherein R’ is alkyl. The term “lower- alkoxy” or “C,7-alkoxy” refers to the group R’-O-, wherein R’ is lower-alkyl. Examples of
Jower-alkoxy groups are e.g. methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy and hexyloxy. Preferred are the lower-alkoxy groups specifically exemplified herein.
The term “alkylthio” refers to the group R’-S-, wherein R’ is alkyl. The term “lower-alkylthio” or “Cy7-alkylthio” refers to the group R*-S-, wherein R’ is lower-alkyl.
Examples of C, 7-alkylthio groups are e.g. methylthio or ethylthio. Preferred are the lower-alkylthio groups specifically exemplified herein.
The term “mono- or di-C,.7-alkyl-amino” refers to an amino group, which is mono- or disubstituted with Cy.7-alkyl. A mono-C7-alkyl-amino group includes for example methylamino or ethylamino. The term “di-C,.7-alkyl-amino” includes for example dimethylamino, diethylamino or ethylmethylamino. Preferred are the mono- or di-C,.;-alkylamino groups specifically exemplified herein.
The term “carboxy-lower alkyl” or “carboxy-Cy.7-alkyl” refers to to lower atkyl groups which are mono- or multiply substituted with a carboxy group (-COOH).
Examples of carboxy-lower alkyl groups are e.g. -CH,-COOH (carboxymethyl), -(CHz)2-
COOH (carboxyethyl) and the groups specifically exemplified herein.
The term “alkanoyl” refers to the group R*-CO-, wherein R’ is alkyl. The term “lower-alkanoyl” or “C,.7-alkanoyl” refers to the group R’-O-, wherein R’ is lower-alkyl.
Examples of lower-alkanoyl groups are e.g. ethanoyl (acetyl) or propionyl. Preferred are the lower-alkoxy groups specifically exemplified herein.
The term “lower alkenyl” or “C,.;-alkenyl”, alone or in combination, signifies a straight-chain or branched hydrocarbon residue comprising an olefinic bond and up to 7, preferably up to 6, particularly preferred up to 4 carbon atoms. Examples of alkenyl groups are ethenyl, 1-propenyl, 2-propenyl, isopropenyl, 1-butenyl, 2-butenyl, 3-butenyl and isobutenyl. A preferred example is 2-propenyl.
The term “lower alkinyl” or “C,.7-alkinyl”, alone or in combination, signifies a straight-chain or branched hydrocarbon residue comprising a triple bond and up to 7, preferably up to 6, particularly preferred up to 4 carbon atoms. Examples of alkinyl groups are ethinyl, 1-propinyl, or 2-propinyl.
The term “cycloalkyl” or “Css-cycloalkyl” denotes a saturated carbocyclic group containing from 3 to 7 carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl.
The term “aryl” relates to the phenyl or naphthyl group, preferably the phenyl group, which can optionally be mono- or multiply-substituted, particularly mono- or di- substituted by halogen, hydroxy, CN, CFs, NO2, NHy, N(H, lower-atkyl), N(lower- alkyl),, carboxy, aminocarbonyl, lower-alkyl, lower fluoroalkyl, lower-alkoxy, lower fluoroalkoxy, aryl and/or aryloxy. Preferred substituents are halogen, CFs, lower-alkyl, and/or lower-alkoxy. Preferred are the specifically exemplified aryl groups.
The term “heteroaryl” refers to an aromatic 5- or 6-membered ring which can ’ 30 comprise 1,2 or 3 atoms selected from nitrogen, oxygen and/or sulphur such as furyl, pyridyl, 1,2-, 1,3- and 1,4-diazinyl, thienyl, isoxazolyl, oxazolyl, imidazolyl, or pyrrolyl.
The term "heteroaryl" further refers to bicyclic aromatic groups comprising two 5- or 6- membered rings, in which one or both rings can contain 1, 2 or 3 atoms selected from nitrogen, oxygen or sulphur such as e.g. indole or quinoline, or partially hydrogenated bicyclic aromatic groups such as e.g. indolinyl. A heteroaryl group may havea substitution pattern as described earlier in connection with the term “aryl”. Preferred heteroaryl groups are e.g. thienyl and furyl which can optionally be substituted as described above, preferably with halogen, CFs, lower-alkyl and/or lower-alkoxy.
The term “protecting group” refers to groups such as e.g. acyl, alkoxycarbonyl, aryloxycarbonyl, benzyl, silyl, or imine-derivatives, which are used to temporarily block the reactivity of functional groups. Well known protecting groups are e.g. t- butyloxycarbonyl, benzyloxycarbonyl, fluorenylmethyloxycarbonyl or diphenylmethylene which can be used for the protection of amino groups, or lower- alkyl, p-trimethylsilylethyl- and p-trichloroethyl-esters, which can be used for the protection of carboxy groups. Protecting groups which can be used for the protection of hydroxy groups are e.g. benzyl, trimethylsilyl or tert-butyldimethylsilyl. “Isomers” are compounds that have identical molecular formulae but that differ in the nature or the sequence of bonding of their atoms or in the arrangement of their atoms in space. Isomers that differ in the arrangement of their atoms in space are termed “stereoisomers”. Stereoisomers that are not mirror images of one another are termed “diastereoisomers”, and stereoisomers that are non-superimposable mirror images are termed “enantiomers”, or sometimes optical isomers. A carbon atom bonded to four nonidentical substituents is termed a “chiral center”.
The term “pharmaceutically acceptable salts” embraces salts of the compounds of formula (I) with pharmaceutically acceptable bases such as alkali salts, e.g. Na- and K- salts, alkaline earth salts, e.g. Ca- and Mg-salts, and ammonium or substituted ammonium salts, such as e.g. trimethylammonium salts. The term "pharmaceutically acceptable salts" also relates to such salts.
The compounds of formula (I) can also be solvated, e.g. hydrated. The solvation can be effected in the course of the manufacturing process or can take placee.g. asa consequence of hygroscopic properties of an initially anhydrous compound of formula (1) (hydration). The term pharmaceutically acceptable salts also includes pharmaceutically acceptable solvates. 30 . The term “pharmaceutically acceptable esters” embraces derivatives of the. ] compounds of formula (1), in which a carboxy group has been converted to an ester.
Lower-alkyl, hydroxy-lower-alkyl, Jower-alkoxy-lower-alkyl, amino-lower-alkyl, mono- or di-lower-alky}-amino-lower-alkyl, morpholino-lower-alkyl, pyrrolidino-lower-alkyl, piperidino-lower-alkyl, piperazino-lower-alkyl, lower-alkyl-piperazino-lower-alkyl and aralkyl esters are examples of suitable esters. The methyl, ethyl, propyl, butyl and benzyl esters are preferred esters. The methyl and ethyl esters are especially preferred. The term “pharmaceutically acceptable esters” furthermore embraces compounds of formula (I) in which hydroxy groups have been converted to the corresponding esters with inorganic or organic acids such as, nitric acid, sulphuric acid, phosphoric acid, citric acid, formic acid, maleic acid, acetic acid, succinic acid, tartaric acid, methanesulphonic acid, p- toluenesulphonic acid and the like, which are non toxic to living organisms.
In detail, the present invention relates to compounds of formula I
RS
R? R® o x R’ I rR. oo X RE
RY R and enantiomers and pharmaceutically acceptable salts and esters thereof, wherein
X!is 0, S, CH;
R! is hydrogen or Cy7-alkyl;
R? is hydrogen or C;.s-alkyl, or, if X! is CH, R? is hydrogen, C,.7-alkyl or Cy.7-alkoxy;
R®is hydrogen or C;.;-alkyl;
R*and R® independently from each other are hydrogen, C.7-alkyl,
Cs.7-cycloalkyl, halogen, C, 7-alkoxy- C,.7-alkyl, C;7-alkenyl, C;7-alkinyl, fluoro-C; ;-alkyl, cyano-Cy.7-alkyl or cyano;
R®, R® and R’ independently from each other are hydrogen, C,.7-alkyl,
Cs.7-cycloalkyl, halogen, Cy.7-alkoxy- C,7-alkyl, C,.7-alkenyl, C.7-alkinyl, fluoro-C,.7-alkyl, cyano-C,.7-alkyl or cyano; and one of R*, R®and R'is To 1 y=Y R" 2 (CR1ORM), ~ (CH), yy! wherein
X2is S, O, NB, (CH,)pNR’CO, or (CH,)sCONR’,
R’ is hydrogen, C,.7-alkyl, Cs.;-cycloallyl, fluoro-C;.7-alkyl, hydroxy-C,.s-alkyl, or
C,7-alkoxy-C,.7-alkyl;
Y, Y% Y? and Y* are N or C-R*? and 1 or 2 of Y., Y2, Y? and Y* are N and the other ones are C-R'%;
Rr is C1.7-alkyl, Cs.5-cycloalkyl, fluoro-C;.s-alkyl, or C,.7-alkoxy-Cy.7-alkyl;
R" is hydrogen, C.7-alkyl, C;.7-alkoxy-Cy-alkyl;
R'%independently from each other in each occurance is selected from hydrogen,
C,.7-alkyl, Csz-cycloalkyl, fluoro-C;.s-alkyl, C,.7-alkoxy-C,.7-alkyl, hydroxy-C,.7-alkyl, Cig alkylthio-C,.7-alkyl, carboxy-C,.7-alkoxy-C,.;-alkyl, carboxy, carboxy-C,.7-alkyl, mono- or di-C,7-alkyl-amino-C,_-alkyl,
C,.7-alkanoyl-C;.7-alkyl, Co7-alkenyl, and C,7-alkinyl;
R" is aryl or heteroaryl; misOorl,nis0,1,20r3,and pis 0, 1 or2,and thesumofm,nand pis 1,2,3 or 4 provided that compounds of formula I are excluded, wherein
X!is O, R? and R® are hydrogen,
R%is equal to 1 ye=Y R' x2 be
GRR, ~ 1 vy
X?isOorS,and misO. ] Preferred compounds of formula I of the present invention are compounds, wherein
X?is NR’, (CH,)sNR’CO or (CH,)yCONR’,
R’ is hydrogen, Cy.7-alkyl, Cs.7-cycloalkyl, fluoro-Cy7-alkyl, hydroxy-Cs.;-alkyl, or
Cy.7-alkoxy-C,.s-alkyl; and pis 0, 1 or 2. wy
Especially preferred are those compounds of formula I in accordance with the present invention, wherein X’ is NR’, and R® is hydrogen, Cy.7-alkyl, Cs.;-cycloalkyl, fluoro-C,.7-alkyl, hydroxy-Ca.7-alkyl, or C,.7-alkoxy-Ca.7-alkyl.
Also preferred are compounds of formula I, wherein X* is (CH,)pNR’CO, or (CH,)pCONR®, rR’ is hydrogen, Ci.7-alkyl, Cs.-cycloalkyl, fluoro-C,.7-alkyl, hydroxy-Co- alkyl, or C, 7-alkoxy-C,7-alkyl, and pis 0, 1 or 2.
Preferred examples of such compounds include the following: 2-methyl-2-(3-{methyl-[2-methyl-6-(4-triftuoromethyl-phenyl)-pyridin-3-ylmethyl)- carbamoyl}-phenoxy)-propionic acid, 2-[3-({2-[4-cyclopropyl-2-(4-triftuoromethyl-phenyl)-pyrimidin-5-yl]-acetyl}-methyl- amino)-phenoxy]-2-methyl-propionic acid, 2-methyl-2-[3-(methyl-{2-[2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-yl}- acetyl}-amino)-5-trifiuoromethyl-phenoxy] -propionic acid, : 2-[4-({ [4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidine-5-carbonyl] -amino}- methyl)-phenoxy]-2-methyl-propionic acid, and 2-methyl-2-(4-{ [2-methyl-6-(4-triftuoromethyl-phenyl) -pyridin-3-ylcarbamoyl]- methyl}-phenoxy)-propionic acid.
Further preferred examples of such compounds are the following: 2-[3-chloro-4-({[4-trifluoromethyl-2- (4-trifluoromethyl-phenyl)-pyrimidine-5- carbonyl]-amino}-methyl)-phenoxy]-2-methyl-propionic acid, 2-methyl-2-[4-({[4-trifluoromethyl-2- (4-trifluoromethyl-phenyl)-pyrimidine-5- carbonyl] -amino}-methyl)-phenoxy]-propionic acid; and 2-(4-{ [4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-ylcarbamoyl] -methyl}- phenoxy)-2-methyl-propionic acid.
Compounds of formula I, wherein X? is NR®, (CH2)pNR’CO or (CH,)sCONR® and
R’ is C,.7-alkyl, are more preferred.
Examples of especially preferred compounds, wherein X?is NR, (CH3)pNR’CO or (CHa2)pCONR?, are the following: (2-(3-methoxy-propyl)-4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ylmethyl]-amino}-phenoxy)-acetic acid, cS [rac]-[2-methyl-4-(methyl-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]- butyl}-amino)-phenoxy]-acetic acid, (4-{[6-(4-chloro-phenyl)-pyridin-3-ylmethyl] -methyl-amino}-2-methyl-phenoxy)-acetic acid, 2-methyl-2-[3-(methyl-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- yr acetyl}-amino)-phenoxy] -propionic acid, and (4- (4-cydlopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethyl] -methyl- amino}-2-methyl-phenoxy)-acetic acid.
Furthermore, compounds of formula I in accordance with the present invention are preferred, wherein xX! and X? are O and R? is C,.s-alkyl, with those compounds of formula I, wherein R? and R? are C,.,-alkyl, being especially preferred.
Examples of such compounds are the following: 2-methyl-2-{2-methyl-4-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-ylmethoxyl- phenoxy}-propionic acid, 2-methyl-2-{2-methyl-4-|6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxyl- phenoxy} -propionic acid, and 2-{4-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid.
Further preferred examples of such kind of compounds include 2-methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3- yimethoxy]-phenoxy}-propionic acid, 2-{4-[4-cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, and 2-{4-[4-(2-methoxy-ethyl)-6-methyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5- ylmethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid.
Furthermore, the following compounds are examples therof: 2-{4-[2-cyclopropyl-6-(4-trifiuoromethoxy-phenyl)-pyridin-3-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, 2-{4-[4-methoxymethyl-6-methyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5- ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4- [2-cyclopropyl-6-(4-triftuoromethoxy-phenyl)-4-trifluoromethyl-pyridin-3- ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-methoxymethyl-2-(4-trifluoromethoxy-phenyl) -pyrimidin-5-ylmethoxy]-2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6-(4-trifluaromethoxy-phenyl)-4-trifluoromethyl-pyridin-3- } ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, and 2-{4-[4-methoxymethyl-2- (4-trifluoromethoxy-phenyl)-pyrimidin-5-ylmethoxy]-2- methyl-phenoxy}-2-methyl-propionic acid.
Further preferred compounds of formula I of the present invention are those, wherein X' and X? are O and mis 1.
Jl
The following compounds, wherein X' and X? are O and m is 1, are especially preferred: [rac]-(4-{ 1-{4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-y1] -ethoxy}-2- methyl-phenoxy)-acetic acid, and [rac]-(4~{1- [4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-y] -butoxy}-2- methyl-phenoxy)-acetic acid. ’
Also preferred are compounds of formula I according the present invention, wherein X' is S.
Examples of such compounds are the following: [rac]-(2-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-yl]-ethoxy}- phenylsulfanyl)-acetic acid, and [rac]-(2-methyl-4-{1- [2-methyl-6-(4-trifluoromethyi-phenyl) -pyridin-3-yl}-butoxy}- phenylsulfanyl)-acetic acid.
Further preferred compounds of formula I according to the present invention are those, wherein X' is O, X*is Sand m is 1.
The following compounds, wherein X!is O, X?is Sand m is 1, are especially preferred: [rac]-(2-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-yl]- ethylsulfanyl}-phenoxy)-acetic acid, and [rac]-(4-{1- [4-Cydopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl}- ethylsulfanyl}-2-methyl-phenoxy)-acetic acid.
Compounds of formula I, wherein R* is aryl, are preferred. More preferred are those compounds of formula I, wherein R" is unsubstituted phenyl or phenyl substituted with one to three groups selected from C,.7-alkyl, C,7-alkoxy, halogen, fluoro-C,7-alkyl, fluoro-Cy.7-alkoxy and cyano, with those compounds, wherein R'? is C,.7-alkyl, C1.7- alkoxy, halogen, fluoro-C;.7-alkyl or cyano, being more preferred, and with those compounds, wherein R' is phenyl substituted with halogen or fluoro-C; 7-alkyl, being particularly preferred. Especially preferred are those compounds, wherein R" is 4- trifluoromethylphenyl. Also preferred are those compounds, wherein RPis4- trifluoromethoxyphenyl. ee
Especially preferred are furthermore compounds of formula I, wherein R' is hydrogen. yr
Preferred compounds of formula I of the present invention are those, wherein Réis y=. R® _ (CH) Ne-Y (CRWRM),, .
These compounds have the formula I-A: 1
R® ye=Y Rr"
Rr xX (CH) Sy. (CR'©RM),,~ n 0 x rR’ rR FR
I-A
However, compounds of formula I-A, wherein X' is O, R? and R® are hydrogen, X* is O or S and m is 0, are excluded.
Furthermore, compounds of formula I are preferred, wherein Ror R’ is v=. Rr" x LI — \
RORY, (CH), y-Y
These compounds have the formula I-B or I-C: ve=Y. Rr" (CH) A by x2 _(CRWR")~ n Y?
RY R® 0 x R red, Rr?
RY R°
I-B or
R® ; rR RS Y&=Y _R® 0 x Ke (CRORN), = YY
RPK RE rR R
I-C . yr
The integer m is 0 or 1, the integer nis 0, 1, 2 or 3 and the integer pis 0, 1 or 2, provided that the sum of m, n and pis 1,2, 3 or 4. Especially preferred are compounds of formula I, wherein the sum of m,n and p is 1.
Y', Y?, Y® and Y* signify N or C-R'%, provided that 1 or 2 of Y,Y%Y and Y¢ are N and the other ones are C-R'%. R"2 independently from each other in each occurance is selected from hydrogen, Ci.5-alkyl, Ca.-cycloalkyl, fluoro-Cy.7-alkyl, C,.7-alkoxy-C,.7- alkyl, hydroxy-Ci-r-alkyl, Cis alkylthio-C,.7-alkyl, carboxy-Cy.7-alkoxy-Cy.7-alkyl, carboxy, carboxy-C,.7-alkyl, mono- or di-C, 7-alkyl-amino-Ci.7-alkyl, Cy.7-alkanoyl-Cyo- alkyl, Cy.7-alkenyl, and C; 7-alkinyl. Preferably, R'? independently from each other in each occurance is selected from hydrogen, Cy 7-alkyl, Cs.7-cycloalkyl, fluoro-C,.7-alkyl,
Cy.7-alkoxy-C;.7-alkyl, hydroxy-C;.7-alkyl, and Ci alkylthio-C; s-alkyl. More preferably,
R's selected from hydrogen, Cy.7-alkyl, and Cs7-cycloalkyl.
Preferred compounds of the present invention are for example those, wherein 1 of
Y!, Y?, Y? and Y* is N and the other ones are C-R', thus meaning compounds containing a pyridyl group.
Especially preferred are those compounds of formula I, wherein Y' is N and Y, Y° and Y* are C-R', e. g. compounds of formula I containing the group
R"2 =N 13 \ /}
R" R'2
Further preferred compounds of the present invention are those, wherein 2 of Y',
Y% Y® and Y*are N and the other ones are C-R'?, thus meaning compounds containing a pyrazinyl group or a pyrimidinyl group or a pyridazinyl group.
Especially preferred are compounds of formula I, wherein Y' and Y* are N and Y? and Y? are C-R', e. g. compounds of formula I containing the pyrimidinyl group
R"2 =N 13 4 )—R Co Ca 12 N
R -
Also preferred are compounds of formula I, wherein Y' and Y° are N and Y? and Y* are C-R'2, e. g. compounds of formula I containing the pyrazinyl group
Co yr rR" = R" = £ 12
R'2is preferably hydrogen, C,.7-alkyl, or Ca.7-cycloalkyl.
Examples of preferred compounds of formula I are the following: (2-(3-methoxy-propyl) -4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ylmethyl]-amino}-phenoxy)-acetic acid, (2-(3-methoxy-propyl) -4-{methyl-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3- ylmethyl]-amino}-phenoxy)-acetic acid, [rac]- [2-(3-methoxy-propyl)-4-(methyl-{ 1- [2-methyl-6-(3-trifluoromethyl-phenyl)- pyridin-3-yl]-ethyl}-amino)-phenoxy}-acetic acid, [rac]-[2-(3-methoxy-propyl)-4-(methyl-{1-[2-methyl-6-(3-trifluoromethy}-phenyl)- pyridin-3-yl]-butyl}-amino) -phenoxy]-acetic acid, [rac]-[2-methyl-4-(methyl-{1- [2-methyl-6-(3-trifluoromethyl-phenyl) -pyridin-3-yl}- butyl}-amino)-phenoxy] -acetic acid, [rac)-(4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]-amino}- 2-trifluoromethyl-phenoxy)-acetic acid, (2,6-dimethyl-4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-ylmethyl]- amino}-phenoxy)-acetic acid, 2-methyl-2-{2-methyl-4-[2-methyl-6-(3-triftuoromethyl-phenyl)-pyridin-3-ylmethoxy]- phenoxy} -propionic acid, 2-methyl-2-{2-methyl-4- [6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy}- phenoxy} -propionic acid, 2-methyl-2-{2-methyl-4- [6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy] - phenoxy}-propionic acid, [rac]-[2-methyl-4-(methyl-{1- [2-methyl-6- (3-trifluoromethyl-phenyl)-pyridin-3-yl] - propyl}-amino)-phenoxy}-acetic acid, (4-4 [6-(4-chloro-phenyl)-pyridin-3-ylmethyl] -methyl-amino}-2-methyl-phenoxy)-acetic acid, (2-methyl-4-{methyl-[6- (4-trifluoromethoxy-phenyl)-pyridin-3-ylmethyl) -amino}- ._ . . phenoxy)-acetic acid, (2-(3-methoxy-propyl)-4-{methyl-[6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethyl]- amino}-phenoxy)-acetic acid, (2-methyl-4-{methyl-[6-(4-trifluoromethyl-phenyl) -pyridin-3-ylmethyl]-amino}- phenoxy)-acetic acid,
CL Cy
(2-(3-methoxy-propyl)-4- (methyl-{6-(4-trifluoromethyl-phenyl)-pyridin-3-yimethyl]- amino}-phenoxy)-acetic acid, [rac)-(2-methyl-4-{1- [2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3 -yi]- ethylsulfanyl}-phenoxy)-acetic acid, [rac]-(2-methyl-4-{1-[2-methyl-6-(4-trifiuoromethyl-phenyl)-pyridin-3-yl]- propylsulfanyl}-phenoxy)-acetic acid, [rac]-(2-methyl-4-{ 1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl] - ethylsulfanyl}-phenoxy)-acetic acid, [rac)-(2-methyl-4-{1-[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl} -ethoxy}- phenylsulfanyl)-acetic acid, [rac]-(2-methyl-4-{1-[2-methyi-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl]-butoxy}- phenylsulfanyl)-acetic acid, [rac]-(2-methyl-4-{1- [2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-yl}- butylsulfanyl}-phenoxy)-acetic acid, [rac]-(4-{cyclopentyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-y1]- methylsulfanyl}-2-methyl-phenoxy)-acetic acid, 2-{4-[6-(4-fluoro-3 ~trifluoromethyl-phenyl)-2-methyl-pyridin-3-ylmethoxy]-2 -methyl- phenoxy}-2-methyl-propionic acid, [rac]-2-methyl-2-(2-methyl-4-{1-[2-methyl-6-( 3-trifluoromethyl-phenyl)-pyridin-3-yl}- ethoxy}-phenoxy)-propionic acid, 2-methyl-2-[3-(methyl-{2-[2-methyl-6- (4-trifiuoromethyl-phenyl)-pyridin-3-y1}- acetyl}-amino)-phenoxy]-propionic acid, (4-{ [4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethyl] -methyl- amino}-2-methyl-phenoxy)-acetic acid, [rac)-(4-{1-[4-Cydlopropyl-2-(4-triftuoromethyl-phenyl)-pyrimidin-5-yl]- ethylsulfanyl}-2-methyl-phenoxy)-acetic acid, [rac]-(4-{1-[4-Cydopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl] -ethoxy}-2- methyl-phenoxy)-acetic acid, [rac]-(4-{1-[4-Cyclopropyl-2-( 4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- butylsulfanyl}-2-methyl-phenoxy)-acetic acid, [rac]-(4-{1- [4-Cydlopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-y1} -butoxy}-2- methyl-phenoxy)-acetic acid, 2-{4-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid, and 0 ST (2-methyl-4-{methyl-[5-(4-trifluoromethyl-phenyl)-pyrazin-2-ylmethyl] -amino}- phenoxy)-acetic acid.
Further examples of preferred compounds include the following: 2-methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]- o phenoxy}-propionic acid, [rac]-2-methyl-2-(2-methyi-4-{1- [2-methyl-6- (4-trifluoromethyl-phenyl) -pyridin-3-yl]- butoxy}-phenoxy)-propionic acid, [rac]-2-methyl-2-(2-methyl-4-{ 1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl}-
propoxy}-phenoxy)-propionic acid,
[rac]-2-methyl-2-(2-methyl-4-{1- [2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] - ethoxy}-phenoxy)-propionic acid,
2-{4- [6-(3-fluoro-4-trifluoromethyl-phenyl)-2-methyl-pyridin-3-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid,
2-methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3- ylmethoxy]-phenoxy}-propionic acid, 2-{4-[2-cyclopropyl-6-(4-trifiuoromethyl-phenyl)-pyridin-3-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, .
[rac)-2-methyl-2-(2-methyl-4-{ 1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]-
butoxy}-phenoxy)-propionic acid, 2-{4-[4-cydlopropyl-2-(3-trifiuoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid,
2-methyl-2-(2-methyl-4-{2-[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl]- ethoxy} -phenoxy)-propionic acid, 2-methyl-2-{2-methyl-4-(6-(4-trifluoromethyl-phenyl)-pyridazin-3-ylmethoxy]- phenoxy}-propionic acid, 2-{4- [2-methoxymethyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, [rac]-(2-methyl-4-{1-[2-methyi-6-(4-trifluoromethyl-phenyl) -pyridin-3-yl]-butoxy}- phenoxy)-acetic acid, [rac]-(2-methyl-4-{1-[2-methyl-6-(3-trifluoromethyl-phenyl) -pyridin-3-yl]-ethoxy}- phenoxy)-acetic acid, [rac]-(2-methyl-4-{1-[2-methyl-6-(3 -trifluoromethyl-phenyl)-pyridin-3-yl]-butoxy}- phenoxy)-acetic acid, [rac]-(2-methyl-4-{1-[2-methy]-6-(4-trifluoromethyl-phenyl)-pyridin-3 -yl]-propoxy}- phenoxy)-acetic acid, [rac]-(2-methyl-4-{1-[2-methyl-6-(4-trifiluoromethyl-phenyl) -pyridin-3-yl]-ethoxy}- phenoxy)-acetic acid, 3-{4-[2-dimethylaminomethyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid, 2-methyl-2-{3-[(methyl-{2- (2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- acetyl} -amino)-methyl]-phenoxy}-propionic acid, 2-[3-({[4-cyclopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidine-5-carbonyl] -methyl- amino}-methyl)-phenoxy]-2-methyl-propionic acid, r
2-methyl-2-[3-({methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine-3-carbonyl}- amino}-methyl)-phenoxy]-propionic acid, 2-methyl-2-{3-(2-{2- [2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl] - acetylamino}-ethyl)-phenoxy) -propionic acid, 2-methyl.2-[3-(2-{[2-methyl-6-(4-triftuoromethyl-phenyl)-pyridine-3-casbonyl]- amino}-ethyl)-phenoxy]-propionic acid, 2-[3-(2-{ [4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl] -amino}- ethyl)-phenoxy] -2-methyl-propionic acid, 2-[3-({2- (4-butyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-acetyl}-methyl-amino)- phenoxy]-2-methyl-propionic acid, 2-methyl-2-(3-{methyl- [2-methyl-6-(4-triffuoromethyl-phenyl) -pyridin-3-ylmethyl]- carbamoyl} -phenoxy)-propionic acid, 3-{4-[4-cyclopropyl-2- (4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy) -2-methyl- phenyl}-propionic acid, 3-{4-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-3-ethoxy- phenyl}-propionic acid, 3-{3-ethoxy-4-(2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-ylmethoxy]-phenyl}- propionic acid, 3-{2-methyl-4-[5-(4-trifluoromethyl-phenyl)-pyrimidin-2-ylmethoxy] -phenyl}- propionic acid, 2-[3-({2- [4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-yl]-acetyl}-methyl- amino)-phenoxy]-2-methyl-propionic acid, 2- [3-(2-(2-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-y1] -acetylamino}- ethyl)-phenoxy]-2-methyl-propionic acid, 2-[3-({[4-cyclopropy}-2-(4-trifinoromethyl-phenyl)-pyrimidine-5-carbonyl]-amino}- methyl)-phenoxy]-2-methyl-propionic acid, 2-methyl-2-[3-({2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-acetylamino}- methyl)-phenoxy]-propionic acid, 2-[3-({2- (4-cyclopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5-yl]-acetylamino}- methyl)-phenoxy]-2-methyl-propionic acid, 2-methyl-2-[3-({ [2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine-3-carbonyi] -amino}- methyl)-phenoxy]-propionic acid, 2-methyl-2-(2-methy}-5-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- ethoxy}-phenoxy)-propionic acid, Cor, re 2-methyl-2-{2-methyl-5-(2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]- phenoxy}-propionic acid, [rac]-3-(2-methyl-4-{1-[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl] -ethoxy}- phenyl)- propionic acid, 3-{3-methyl-4-(2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy] -phenyl}-
Fi propionic acid, [rac] 3-(3-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] -ethoxy}- phenyl)-propionic acid, 2-[3-({2- [4-cyclopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5-yl]-acetyl} -methyl-
amino)-5-trifluoromethyl-phenoxyl -2-methyl-propionic acid,
3-methyl-2-[3-(methyl-{2-[2-methy}-6-(4-trifluoromethyl-phenyl)-pyridin-3-yll- acetyl}-amino)-5-trifluoromethyl-phenoxy) -propionic acid,
2-[4-({2- [4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-y1]-acetylamino}-
methyl)-phenoxy]-2-methyl-propionic acid,
2-methyl-2-[4-({2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi]-acetylamino}-
methyl)-phenoxy]-propionic acid,
2-[4-({[4-cyclopropyl-2- (4-trifluoromethyl-phenyl) -pyrimidine-5-carbonyl]-amino}- methyl)-phenoxy)-2-methyl-propionic acid,
2-methyl-2-[4-({ [2-methyl-6-(4-trifluoromethyl-phenyl) -pyridine-3-carbonyl]-amino}-
methyl)-phenoxy]-propionic acid, 2-methyl-2-(3-{methyi-[4-methyl-2- (4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethyl} - carbamoyl} -phenoxy)-propionic acid, 2-(3-{[4-(2-methoxy-ethyl)-6-methyl-2- (4-trifluoromethyl-phenyl)-pyrimidin-5- ylmethyl] -methyl-carbamoyl}-phenoxy)-2-methyl-propionic acid,
2-methyl-2-{4-[ (methyl-{2-[2-methy}-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi}- acetyl}-amino)-methyl]-phenoxy} -propionic acid, 2-methyl-2-[4-(2-{[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridine-3-carbonyl]- amino}-ethyl)-phenoxy] -propionic acid, 2-[4-(2-{[4-cyclopropyl-2- (4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl] -amino}-
ethyl)-phenoxy]-2-methyl-propionic acid, 2-methyl-2-[4-(2-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- acetylamino}-ethyl)-phenoxy}-propionic acid, 2-[4-(2-{2- [4-cydopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5-yl]-acetylamino}- ethyl)-phenoxy] -2-methyl-propionic acid,
2-methyl-2-{4-[2-(methyl-{2-[2-methy}-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi]- acetyl}-amino)-ethyl]-phenoxy}-propionic acid, 2-methyl-2-[4-(2-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine-3-
carbonyl]-amino}-ethyl)-phenoxy]-propionic acid, 3-{2-methoxy-4-[2-methyl-6-(4-triftuoromethyl-phenyl)-pyridin-3-ylmethoxy] oo phenoxy}-2-methyl-propionic acid,
[rac]-2-(2-methoxy-4-{1- [2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- ethoxy}-phenoxy)-2-methyl-propionic acid, 3-{2-methoxy-4-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy}- phenyl}-propionic acid,
y
2-[3-methoxy-5-(2-{[2-methyl-6-(4- triftuoromethyl-phenyl)-pyridine-3-carbonyl]- amino}-ethyl)-phenoxy]-2 -methyl-propionic acid, 2-[3-(2-{[4-cyclopropyl-2- (4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl] -amino}- ethyl)-5-methoxy-phenoxy] -2-methyl-propionic acid,
3-[3-(2-{2-{4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yi]-acetylamino}- ethyl)-5-methoxy-phenoxy] -2-methyl-propionic acid, 2-[3-methoxy-5-(2-{2-[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl]- acetylamino}-ethyl)-phenoxy] -2-methyl-propionic acid, 2-methyl-2-[2-methyl-4-({{2-methyl-6- (4-trifluoromethyl-phenyl)-pyridine-3-
carbonyl}-amino}-methyl)-phenoxy]-propionic acid,
2-[4-({ [4-cyclopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidine-5-carbonyl]-amino}- methyl)-2-methyl-phenoxy] -2-methyl-propionic acid,
2-[4-({[4-methoxymethyl-2- (4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl] - amino}-methyl)-2-methyl-phenoxy]-2-methyl-propionic acid,
2-[4-({[4-(2-methoxy-ethy})-2-(4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl]- amino}-methyl)-2-methyl-phenoxy] -2-methyl-propionic acid, 2-{4-[4-(2-ethoxy-ethyl)-2-(6-trifluoromethyl-pyridin-3-yl)-pyrimidin-5-ylmethoxy] -2-
: methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-(2-methoxy-ethyl)-2-(6-trifluoromethyl-pyridin-3-yl)-pyrimidin-5-ylmethoxy]-
2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-(2-hydroxy-ethyl) -2-(6-trifluoromethyl-pyridin-3-yl)-pyrimidin-5-yimethoxy] - 2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-methoxymethyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-2- methyl-phenoxy}-2-methyl-propionic acid,
2-{4- [4-cyclopropyl-2-(6-trifluoromethyl-pyridin-3-yl)-pyrimidin-5-ylmethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-cyclopropyl-2-(4-trifuoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2,3-
dimethyl-phenoxy}-2-methyl-propionic acid, 2-methyl-2-{2-methyl-4-(4-trifluoromethyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-
ylmethoxy]-phenoxy}-propionic acid, 2-methyl-2-{2-methyl-4- [4-trifluoromethyl-6-(4-triftuoromethyl-phenyl)-pyridin-3- ylmethoxy]-phenoxy}-propionic acid, 2-{4-[4-chloro-6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy] -2-methyl-
) phenoxy}-2-methyl-propionic acid, oT
2-{4-(4-(2-hydroxy-ethyl)-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yimethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid, 2-methyl-2-{2-methyl-4-[2-(4-trifluoromethoxy-phenyl)-4-trifluoromethyl-pyrimidin- 5-ylmethoxy]-phenoxy}-propionic acid, 2-methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethoxy-phenyl)-4-trifluoromethyl-
pyridin-3-ylmethoxy] -phenoxy}-propionic acid, 2-{4- [4-cyclopropyl-6-methoxymethyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5- yimethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4- (4-cyclopropyl-6-methoxymethyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-
ylmethoxy]-phenoxy}-2-methyl-propionic acid,
2-{4- [4-butyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yimethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, 2-{4-[4-butyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -phenoxy}-2- methyl-propionic acid,
3-{4-[4-cyclopropyl-2-(4-triftuoromethyl-phenyl)-pyrimidin-5-yimethoxy)-phenoxy}-2-
methyl-propionic acid,
2-{4-[4-(2-methoxy-ethyl) -6-methyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5- ylmethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-(2-ethoxy-ethyl)-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2-
methyl-phenoxy}-2-methyl-propionic acid and 2-{4-[4-(2-methoxy-ethyl)-2-(4- trifluoromethyl- phenyl)-pyrimidin-5-yimethoxy] -2-methyl-phenoxy}-2-methyl- propionic acid (3:2), [rac]-3-(2-methyl-4-{3-methyl-1-[2-methyl-6-(4-trifluoromethy}-phenyl)-pyridin-3-yi]- butoxy}-phenyl)-propionic acid,
2-[4-({ [4-methoxymethyl-2-(4-trifluoromethyl-phenyl) -pyrimidine-5-carbonyl]- amino}-methyl)-phenoxy]-2-methyl-propionic acid, 2-[4-({[4-(2-methoxy-ethyl)-2- (4-triflucromethyl-phenyl)-pyrimidine-5-carbonyl}- amino}-methyl)-phenoxy]-2-methyl-propionic acid, 2-(3-methoxy-5-{methyl-[2-methyl-6-(4-triflucromethyl-phenyl) -pyridin-3-ylmethyl]-
carbamoyl}-phenoxy)-2-methyl-propionic acid, 2-methyl-2-[4-({[2-methyl-6-(4-triftuoromethyl-phenyl)-pyridin-3-ylmethyl}- carbamoyl}-methyl)-phenoxy]-propionic acid, 2-methyl-2-(4-{ [2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-ylcarbamoyl]- methyl}-phenoxy)-propionic acid,
2-[3-chloro-4-({[2-methy}-6-(4-trifluoromethyl-phenyl)-pyridine-3-carbonyl]-amino}- methyl)-phenoxy]-2-methyl-propionic acid, 2-[3-chloro-4-({[4-cyclopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidine-5-carbonyl]- amino}-methyl)-phenoxy]-2-methyl-propionic acid,
5-[3-chioro-4-({[4-methoxymethyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5- -
carbonyl]-amino}-methyl)-phenoxy] -2-methyl-propionic acid, and 2-[3-chloro-4-({[4-(2-methoxy-ethyl)-2- (4-trifluoromethyl-phenyl)-pyrimidine-5- carbonyl]-amino}-methyl) -phenoxy]-2-methyl-propionic acid.
Further examples of preferred compounds are the following: 2-(3-{ [4-cyclopropyl-2-(4-trifiuoromethyl-phenyl) -pyrimidin-5-ylmethyl] -methyl- carbamoyl} -5-methoxy-phenoxy)-2-methyl-propionic acid, 2-methyl-2-[4-( {methyl-[2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-yi}- carbamoyl}-methyl)-phenoxy]-propionic acid, 2-[3-chloro-4-({[4-trifluoromethyl-6- (4-trifluoromethyl-phenyl)-pyridine-3-carbonyl] - amino}-methyl)-phenoxy] -2-methyl-propionic acid, 2-[3-chloro-4-({[4-triffluoromethyl-2- (4-trifiuoromethyl-phenyl)-pyrimidine-5- carbonyl]-amino}-methyl)-phenoxy] -2-methyl-propionic acid, -methyl-2-[4-({[4-trifluoromethyl-6-(4-triftuorometbyl-phenyl)-pyridine-3-carbonyl]- amino}-methyl)-phenoxy]-propionic acid, 2-methyl-2-{4-({ [4-trifluoromethyl-2-(4-trifluoromethyl-phenyl) -pyrimidine-5- carbonyl]-amino}-methyl)-phenoxy] -propionic acid, 2-(4-{[4-cyclopropyl-2- (4-trifluoromethyl-phenyl)-pyrimidin-5-ylcarbamoyl] -methyl}- phenoxy)-2-methyl-propionic acid, 2-(3-{[4-cyclopropyl-2- (4-trifiuoromethyl-phenyl) ~pyrimidin-5-ylcarbamoyl] -methyl}- phenoxy)-2-methyl-propionic acid, 2-methyl-2-(3-{ [2-methyl-6-(4-trifluoromethyl-phenyl) -pyridin-3-ylcarbamoyl]- methyl}-phenoxy)-propionic acid, 2-methyl-2-(4-{[4-trifluoromethyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ylcarbamoyl]-methyl}-phenoxy)-propionic acid, 2-{4- [4-(2-methoxy-ethyl)-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy) -2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-3-fluoro-
phenoxy}-2-methyl-propionic acid, 2-{4-[4-cyclopropyl-2-(4-trifluoromethoxy-phenyl) -pyrimidin-5-ylmethylsulfanyl}-2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6-(4-trifluoromethoxy-phenyl) -pyridin-3-ylmethylsulfanyl}-2- methyl-phenoxy}-2-methyl-propionic acid,
2-{4-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethylsulfanyl]-2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6-(4-trifuoromethoxy-phenyl) -pyridin-3-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid,
{2-methyl-4-[2-rmethyl-6-(4-triftuoromethyl-phenyl)-pyridin-3-ylmethoxy}- ST phenylsulfanyl}-acetic acid, 2-{4-[4-methoxymethyl-6-methyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5- ylmethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid,
2-{4- [4-methoxymethyl-6-methyl-2-(3-trifluoromethyl-phenyl) -pyrimidin-5- yimethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid,
2-{4-[4-methoxymethyl-6-methyl-2-(4-trifiuoromethoxy-phenyl)-pyrimidin-3- ylmethoxy] _2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[4-cyclopropyl-2- (4-trifluoromethoxy-phenyl) -pyrimidin-5-ylmethoxy) -2-methyl- phenoxy}-2-methyl-propionic acid, 2-methyl-2-{2- methyl-4-[6-(4-trifluoromethoxy-phenyl)-4-triftuoromethyl-pyridin-3- ylmethoxy]-phenoxy}-propionic acid, 2-{4- [2-cyclopropyimethyl-6-(4-trifluoromethoxy-phenyl) -4-trifluoromethyl-pyridin-3- ylmethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6- (4-trifluoromethoxy-phenyl)-4-trifluoromethyl-pyridin-3- ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, >-methyl-2-{2-methyl-4-[6-(4-trifluoromethoxy-pheny})-2-trifuoromethyl-pyridin-3- yimethoxy]-phenoxy}-propionic acid, and 3-{4-[4-methoxymethyl-2-(4-trifizoromethoxy-phenyl)-pyrimidin-5-ylmethoxy} -2- methyl-phenoxy}-2-methyl-propionic acid.
Particularly preferred compounds of formula I of the present invention are the following: 2-methyl-2-{2-methyl-4- [2-methyl-6-(3-triftuoromethyl-phenyl)-pyridin-3 -ylmethoxy]- phenoxy} -propionic acid, 2-methyl-2- {2-methyl-4-[6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy]- phenoxy}-propionic acid, [rac]-(2-methyl-4-{1- (2-methyl-6-(4-triftuoromethyl-phenyl) -pyridin-3-yl]-ethoxy}- phenylsulfanyl)-acetic acid, 2-methyl-2-[3-(methyl-{2-[2-methyl-6-(4-triftuoromethyl-phenyl)-pyridin-3-yi}- acetyl}-amino)-phenoxy}-propionic acid, (4-{[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-ylmethyl]-methyl- amino}-2-methyl-phenoxy)-acetic acid, [rac)-(4- 1-[4-Cydopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- ethylsulfanyl}-2-methyl-phenoxy) -acetic acid, [rac]-(4-{ 1-{4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yi] -ethoxy}-2- methyl-phenoxy)-acetic acid, 2-{4-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid, 2-methiyl-2-{2-methyl-4-[2-methyl-6-(4-trifliiofomethoxy-phetiyl)-pyridin-3- ylmethoxy]-phenoxy}-propionic acid, 2-{4-[4-cydlopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2-methyl- phenoxy} -2-methyl-propionic acid, 2.{4-[4-(2-methoxy-ethyl)-6-methyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5- yimethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid,
Co y
2-methyl-2-(3-{methyl- (2-methyl-6-(4-triftuoromethyl-phenyl)-pyridin-3-yimethyl]- carbamoyl} -phenoxy)-propionic acid, 2-[3-({2- [4-cydlopropyl-2-(4-triftuoromethyl- phenyl)-pyrimidin-5-yl] -acetyl}-methyl- amino)-phenoxy] -2-methyl-propionic acid, 2. methyl-2-[3-(methyl-{2-[2-methyl-6-(4-trifluoromethy}-phenyl)-pyridin-3-yl}- acetyl}-amino)-5-trifluoromethyi-phenoxy] -propionic acid, 2-[4-({[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl] -amino}- methyl)-phenoxy] -2-methyl-propionic acid, 2-methyl-2-(4-{[2-methyl-6- (4-trifluoromethyl-phenyl) -pyridin-3-ylcarbamoyl]- methyl}-phenoxy)-propionic acid, 2-[3-chloro-4-({[4-trifluoromethyl-2- (4-trifluoromethyl-phenyl)-pyrimidine-5- carbonyl] -amino}-methyl)-phenoxy] -2-methyl-propionic acid, 2-methyl-2-[4-({ [4-trifluoromethyl-2-(4-trifluoromethyl-phenyl) -pyrimidine-5- carbonyl]-amino}-methyl) -phenoxy]-propionic acid, 2-(4-{[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)- pyrimidin-5-ylcarbamoyl] -methyl}- phenoxy)-2-methyl-propionic acid, -{4-(2-cyclopropyl-6- (4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, 2-{4- [4-methoxymethyl-6-methyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5- ylmethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6-(4-trifluoromethoxy-phenyl)-4-trifluoromethy}-pyridin-3- ylmethoxy)-2-methyl-phenoxy} -2-methyl-propionic acid, 2-{4- [4-methoxymethyl-2-(4-trifluoromethoxy-phenyl)-pyrimidin-5-ylmethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6-(4-triftuoromethoxy-phenyl)-4-trifluoromethyl-pyridin-3- ylmethoxy] -2-methyl-phenoxy}-2-methyl-propionic acid, and 2-{4- [4-methoxymethyl-2-(4-trifluoromethoxy-phenyl)-pyrimidin-5-ylmethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid.
Furthermore, the pharmaceutically acceptable salts of the compounds of formula I and the pharmaceutically acceptable esters of the compounds of formula I individually constitute preferred embodiments of the present invention. - "Compounds of formula I can have one or more asymmetric carbon atoms andcan exist in the form of optically pure enantiomers, mixtures of enantiomers such as, for example, racemates, optically pure diastereoisomers, mixtures of diastereoisomers, diastereoisomeric racemates or mixtures of diastereoisomeric racemates. The optically active forms can be obtained for example by resolution of the racemates, by asymmetric
Co synthesis or asymmetric chromatography (chromatography with a chiral adsorbens or eluant). The invention embraces all of these forms.
It will be appreciated, that the compounds of general formula | in this invention may be derivatised at functional groups to provide derivatives which are capable of conversion back to the parent compound in vivo. Physiologically acceptable and metabolically labile derivatives, which are capable of producing the parent compounds of general formula I in vivo are also within the scope of this invention.
A further aspect of the present invention is the process for the manufacture of compounds of formula I as defined above, which process comprises a) reacting a compound of formula
Rr 6
RY = R 0 xX Rr’ n 1
R~ oo X R® wherein R! is Cy 7-alkyl, R? to R® are as defined above and one of R>, Ror R is selected from -OH, -SH or -NHR’, wherein R’ is as defined above, with a compound of formula 1
Y= _R®
R' As be m
N (CHI NY? — 2/n a 5 (CR1OR11) wherein Y! to Y4, R'%, R", R"®, m and n are as defined above and Ris —OH, -Cl, -Br, -1 or another leaving group, to obtain a compound of formula
RS
R? R° (0) x! R’ 1-1 ) ha 1
R ~ o> X R®
RZ R wherein one of R%, R® and R is
Co .
v=. R"
PP I
~~ CCH NY (CRIRM) and wherein X2 is 0, S or -NR’, R' is C, 7-alkyl and X,Y toY, R*toR® and mand n are as defined above, and optionally hydrolysing the ester group to obtain a compound of formula I, wherein
R!ishydrogen; or, alternatively, : b) reacting a compound of formula
R® 6
RY R o x Rr’ v 1
RN
RY R wherein R! is C;_7-alkyl, R? to R® are as defined above and one of R3, Ror R’ is ~(CHz),-NHR’, wherein R® and p are as defined above, with a compound of formula 1 ve=Y 13
R
0 \4
IQ (CH,) A &
HO (CR1RY) ~~ 2n Y? wherein Y! to Y4, RY, RY, R'?, m and n are as defined above, to obtain a compound of formula
Re
RY R® 1 1-2 0 Xx Rr dod 2
R R wherein one of R>, R® and R’ is v=. rR" x A lg ~~ _ CH)” N\3-Y (CRIORM), and wherein X2 is -(CH;),-NR°CO-, R' is Ci7-alkyl and X', Y' to Y, R?toR? and m,n and p are as defined above, and optionally hydrolysing the ester group to obtain a compound of formula I, wherein
R'is hydrogen; or, alternatively, ¢) reacting a compound of formula
RS
6
R? R 0 x Rr vi 1
R~ oo R® rR R wherein R! is C;.7-alkyl, R to R® are as defined above and one of R®, Ror R’ is —(CHa),-COOH, and p is defined above, with a compound of formula 1 ve=Y 13 ! \Y
N _ (CH), ™ N\e-Y
H™ > (CR™RM) wherein Y! to Y4, R%, RR", R” , m and n are as defined above, to obtain a compound of formula
R
R® rR 0 x! R’ 1-3 1
R~ oo \ r® 2
R R wherein one of R*, RS and Ris v= rR" x2 A )e
IN _(CHYy Na! (CR1ORM) and wherein X? is -(CHz),-CONR’, R' is Cy.7-alkyl and XX, Y'to YL, R*toR® and m,n and p are as defined above, and optionally hydrolysing the ester group to obtain a compound of formula I, wherein
R!ishydrogen.
As described above, the compounds of formula (I) of the present invention can be used as medicaments for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPARa. agonists. Examples of such diseases are diabetes, particularly non-insulin dependent diabetes mellitus, increased lipid and cholesterol levels, particularly low HDL-cholesterol, high LDL-cholesterol, or high triglyceride levels, atherosclerotic diseases, metabolic syndrome (syndrome X), obesity, elevated blood pressure, endothelial dysfunction, procoagulant state, dyslipidemia, polycystic ovary syndrome, inflammatory diseases (such as e.g. Crohn’s disease, inflammatory bowel disease, colitis, pancreatitis, cholestasis/fibrosis of the liver, rheumatoid arthritis, osteoarthritis, psoriasis and other skin disorders, and diseases that have an inflammatory component such as e.g. Alzheimer’s disease or impaired/improvable cognitive function) and proliferative diseases (cancers such as e.g. liposarcoma, colon cancer, prostate cancer, pancreatic cancer and breast cancer). The use as medicament for the treatment of low
HDL cholesterol levels, high LDL cholesterol levels, high triglyceride levels, and the metabolic syndrome (syndrome X) is preferred.
The invention therefore also relates to pharmaceutical compositions comprising a compound as defined above and 2 pharmaceutically acceptable carrier and/or adjuvant.
Further, the invention relates to compounds as defined above for use as therapeutically active substances, particularly as therapeutic active substances for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPARa agonists. Examples of such diseases are diabetes, particularly non-insulin dependent diabetes mellitus, increased lipid and cholesterol levels, particularly low HDL-cholesterol, high LDL-cholesterol, or high triglyceride levels, atherosclerotic diseases, metabolic - syndrome (syndrome X), obesity, elevated blood pressure, endothelial dysfunction, procoagulant state, dyslipidemia, polycystic ovary syndrome, inflammatory diseases such as rheumatoid arthritis, osteoarthritis, psoriasis and other skin disorder, and proliferative diseases.
Co .
In another embodiment, the invention relates to a method for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPAR0. agonists, which method comprises administering a compound of formula (I) to 2 human or animal.
Preferred examples of such diseases are diabetes, particularly non-insulin dependent diabetes mellitus, increased lipid and cholesterol levels, particularly low HDL-cholesterol, high LDL-cholesterol, or high triglyceride levels, atherosclerotic diseases, metabolic syndrome (syndrome X), obesity, elevated blood pressure, endothelial dysfunction, procoagulant state, dyslipidemia, polycystic ovary syndrome, inflammatory diseases such as rheumatoid arthritis, osteoarthritis, psoriasis and other skin disorder, and proliferative diseases.
The invention further relates to the use of compounds as defined above for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPARG agonists. Preferred examples of such diseases are diabetes, particularly non-insulin dependent diabetes mellitus, increased lipid and cholesterol levels, particularly low HDL- cholesterol, high LDL-cholesterol, or high triglyceride levels, atherosclerotic diseases, metabolic syndrome (syndrome X), obesity, elevated blood pressure, endothelial dysfunction, procoagulant state, dyslipidemia, polycystic ovary syndrome, inflammatory diseases such as rheumatoid arthritis, osteoarthritis, psoriasis and other skin disorder, and proliferative diseases. i
In addition, the invention relates to the use of compounds as defined above for the preparation of medicaments for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPARa. agonists. Preferred examples of such diseases are diabetes, particularly non-insulin dependent diabetes mellitus, increased lipid and cholesterol levels, particularly low HDL-cholesterol, high LDL-cholesterol, or high triglyceride levels, atherosclerotic diseases, metabolic syndrome (syndrome X), obesity, elevated blood pressure, endothelial dysfunction, procoagulant state, dyslipidemia, polycystic ovary syndrome, inflammatory diseases such as theumatoid arthritis, osteoarthritis, psoriasis and other skin disorder, and proliferative diseases. Such medicaments comprise a compound as defined above.
The compounds of formula I can be manufactured by the methods given below, by the methods given in the examples or by analogous methods. Appropriate reaction conditions for the individual reaction steps are known to a person skilled in the art.
Starting materials are either commercially available or can be prepared by methods analogous to the methods given below, by methods described in references cited in the text or in the examples, or by methods known in the art. -
The synthesis of compounds with the general structure I, particularly compounds according to formula Ia to Iq, are described in scheme 1 to scheme 7. Scheme 8 to scheme 11 describe the synthesis of heterocycles 5 (scheme 1), identical to 11 (scheme 3), 8 (scheme 5) and 8 (scheme 6); 5 (scheme 4) identical to 8 (scheme 7) and 6 (scheme 4) identical to 9 (scheme 7).
The synthesis of compounds with the general structure I, particularly compounds according to formula Ia with X! and X? equal to oxygen can be accomplished according to scheme 1.
Scheme 1 5 AkylH ?
R ky! RA ) — R AkyVH
RY ray RY 0) 2 0 7 a a 7
HO R RL 0 R
R® O R®
RY R
1 > 3
R®
RY OH
Oo lo} 7
R! R “oo X R® yv=Y. rR"
Rl (CH A Lg 4 (CR1RM),~ 2h Y*- c 5 1
R® a il " 0 /
R ~~ _ cy 0 ye-Y* (CR1IORM)
Lo i 0) 0 Rr’ - - C— . R
RL oA R®
Ia - Ir
Hydroxy aldehydes or hydroxy aryl alkyl ketones 1 are known or can be prepared by methods known in the art. Reaction of phenols 1 with alpha halo esters of formula 2 in the presence of a base like potassium or cesium carbonate in solvents like acetone, methyl-ethyl ketone, acetonitrile or N,N-dimethylformamide in a temperature range between room temperature and 140 °C leads to the corresponding ether compounds 3 (steps a). Baeyer Villiger oxidation e. g. with meta chloro perbenzoic acid in a solvent like dichloromethane, leads to compounds 4 (step b). Heterocycles 5 (prepared as outlined in schemes 8 to 11) are condensed with phenols 4 according to well known procedures (step ¢): if RM represents a hydroxy group e. g. via Mitsunobu-reaction, with triphenylphosphine and di-tert-butyl-, diisopropyl- or diethyl-azodicarboxylate as reagents; this transformation is preferably carried out in a solvent like toluene, dichloromethane or tetrahydrofuran at ambient temperature. Alternatively, if R* represents a halide, mesylate, tosylate or triflate moiety, the heterocycles 5 can be reacted with phenols 4 in solvents like N,N -dimethylformamide, dimethylsulfoxide, acetonitrile, acetone or methyl-ethyl ketone in the presence ofa weak base like cesium or potassium carbonate at a temperature ranging from room temperature to 140 °C, preferably around 50 °C to yield ether compounds Ia (step ¢). Those can optionally be hydrolyzed according to standard procedures, e. g. by treatment with an alkali hydroxide like LOH or NaOH in a polar solvent mixture like tetrahydrofuran/ethanol/water leading to carboxylic acids
Ia.
An analogous reaction scheme with the same reaction sequences applies for the isomeric compound series leading to compounds of general formula I, particularly compounds according to formula Ib:
R®
RA RE =e Rr
RSor R7= —O (CH) Sy 0 0 rR (CRVRM)~ n
RL A. R®
RY R
Ib
The synthesis of compounds with the general structure I, particularly compounds "according to formula Ic, with X* equal to O and X* equal to nitrogen canbe accomplished according to schemes 2 and 3.
Scheme 2
R¢ Rr R*
HO Ra pee b So _— JO +O . ; “T¥ TE 1 2 3 .
R . R*
RU Hoes orTrifiate CX 4 oe hod
RFR R® NH, Re NH,
R 4 R e 6
R* J rR. Hs orTriflate LC rR R r® i N° 5 RO 4 , HO Te g Soe LA
LIN ore or Trifiate CL 1, Je oo. x BW rR rR’ 5 8 7
Nitro-phenols 2 of scheme 2 are commercial available, or known or can be synthesized from anisols 3 by demethylation with aqueous 62% HBr in acetic acid 5 between RT and 120 °C (step b). Alternatively, phenols 1 can be nitrated in para-position according to well established methods, e. g. with a solution of NaNOQs in water/concentrated hydrochloric acid in a solvent like Et;0, followed by the addition of acetic acid anhydride at RT [following a procedure of P. Keller, Bull. Soc. Fr. 1994, 131, 27-29] leading to phenols 2 (step a). Nitro-phenols 2 are then hydrogenated in an alcohol like EtOH or MeOH with hydrogen in the presence of Pd/C and optionally an acid like
HCl or AcOH at RT to give anilines 4 (step c). Intermediates 4 are then alkylated at oxygen with an activated ester compound 5, e.g. a bromo-acetate 5, in the presence of
K5CO;s or Cs;CO:s in a solvent like acetonitrile or acetone between 10 °C and RT to give intermediates 6 of scheme 2 (step d). Activated esters 5 are commercial available or can be synthesized by methods known in the art. Triflates 5 can be prepared from the corresponding alcohols. Anilines 6 can alternatively be synthesized from compounds 5 and nitrophenols 2 in a two step procedure: first by O-alkylation as described above, followed by hydrogenation with Pd/C in an alcohol like MeOH or EtOH optionally in the presence of ACOH or HCI (step €). BOC-protection with di-tert-butyl dicarbonate in tetrahydrofuran at RT to reflux yields compound 7 (step £). Compound 7 can also be synthesized directly from activated esters 5 and BOC-protected aniline 8 with K,CO; or
Cs,COs as described for the synthesis of compounds 6 (step §)-
Intermediates 7 of scheme 3 can optionally be alkylated at nitrogen using sodium hydride and a reactive alkyl halogenide/mesylate or triflate to give compounds 9 (step hs scheme 3). Standard BOC-deprotection (TFA/CHCly, or HCl in dioxane) at 0 °C to RT affords anilines 10 of (step i, scheme 3). Reaction with activated heterocycles 11(R being a halide or a methanesulfonate) using sodium hydride or sodium, potassium or cesium carbonate in N,N-dimethylformamide, dimethylsulfoxide, dimethylacetamide or tetrahydrofuran, at0 °C to RT, leads to compounds Ic (step Kk). Alternatively, heterocycles 11 with R¥=0H can be transformed in situ to the coresponding triflate by treatment with trifluoromethanesulfonic anhydride/2,6-di-tert-butylpyridine in CHCl, at 0 °C. This triflate is then reacted with anilines 10 in the presence of 2,6-di-tert- butylpyridine as base in nitromethane between RT and 60 °C to yield compounds Ic [following a procedure of Belostotskii, Anatoly M., Hassner, A., Ti etrahedron Lett. 1994, 35(28), 5075-6) (step k). Secondary aniline compounds Ic (R’=H) can be reductively methylated with an aqueous solution of NaH,PO; and formaldehyde between RT and 65 °C [Loibner, H., Pruckner, A., Stuetz, A., Tetrahedron Lett. 1984, 25, 2535-2536] to give compounds Ic with R°=Me. Ensuing hydrolysis with aqueous LiOH, NaOH or KOH in tetrahyrofuran/EtOH or another suitable solvent produces compounds Ic of scheme 3 in the form of the free acid.
Scheme 3 0] R* h 0 rR?
Ror RS - RY, Ie R
FCT TCL Kk
RE oo fo) R® 0
Rr’ RF FR 7 9 i 0 R* — RA o Ao R® 2° 3
R™ Ry a rR’ 10 v=Y. RP
Ri A bes jr — (CHa) y-Y k 11 fo R*
RN 075° R®
LC] 1
RZ R R Ye=Y Re
Ly _ CH NY? (CRIORM), le
An analogous reaction scheme with the same reaction sequences applies for the isomeric compound series leading to compounds of general formula I, particularly compounds according to formula Id: o] R* : =y “
AN R 5 7 BS Le ) RSor R7="; N (CH.). =
RR ® R® RN crore,” CH YY
R rR’ id
As alternative to the sequences described in scheme 2, the nitrogen containing intermediates can be prepared from suitable intermediates carrying a phenolic hydroxyl moiety. In such intermediates, optionally carrying one or more protective functions, the phenolic OH group can be replaced by the corresponding aromatic NH function by methods known in the art. For example by a three step sequence as described in
Tetrahedron Letters 43(42),7617-7619(2002): i) transformation of the phenol moiety into its trifluoromethanesulfonate (triflic anhydride, 2,6-lutidine, 4-dimethylamino- pyridine, dichloromethane, 0 °C to room temperature; ii) treatment of the triflate with benzophenone imine, di-palladium-tris(dibenzylideneacetone) complex, S-(-)-2,2'- bis(diphenylphosphino)-1,1'-binaphthyl, cesium carbonate, toluene, in a Schlenk tube at temperatures around 120 °C; iii) treatment with catalytic amounts of hydrochloric acid in wet tetrahydrofuran preferably at room temperature to liberate the aromatic NH2 moiety.
The synthesis of compounds with the general structure 1, particularly compounds according to formula Ie and If, with X' equal to O and X* equal to (CH,),NR’CO, or (CH,),CONR’ can be accomplished according to schemes 4.
: Scheme 4
Re : rR
Re Sw Bes 1 [0] 7 rR BY . R odd pe : rR R? RY ® 1 2
J
’ rR rR ” : oh Rt : a ) OH
R. Le Rr ¥ R Le rR hb
So RNs Re ~o RNs R® 3 4 =r. 13 \ fo} rR p or
ON a roe rman, “EY
S 6
RS i) Eh e 'y (C NS
RL BE I R ©
RR?
If 3] rR? YEN oR
LX “4 X nm I WY o 9 1 0 rR R
RN oX ? Ie
Nitriles 1 and aldehydes 2 can be prepared from the corresponding cyano- or formyl-phenols (which are known, commercially available or can be prepared by methods known in the art) by reaction with activated esters compounds (compounds 5 in scheme 2) in the presence of a base like potassium or cesium carbonate in solvents like acetone, methyl-ethyl ketone, acetonitrile or N,N-dimethylformamide in a temperature range between room temperature and 140 °C. Hydrogenation of nitrile compounds 1, e. g. with palladium on charcoal in a mixture of acetic acid and ethanol, or, alternatively, transformation of aldehydes 2 into the corresponding oximes followed by reduction with zinc in acetic acid preferably around 65 °C gives compounds 3 with p = 1 (steps a, c). The preparation of compounds 3 with p=0 has been described in scheme 2 and 3
(compounds 6, scheme 2, and compounds 10, scheme 3). Compounds 3 with p =2 can be prepared from compounds 2 in a two step procedure: i) treatment with nitro-methane and ammonium acetate in acetic acid at a temperature around 110 °C to form the corresponding nitro styrene compounds; ii) hydrogenation with palladium on charcoal in the presence of a strong acid like sulfuric acid or hydrogen chloride in solvents like ethanol or acetic acid and in a temperature range between room temperature and 100 °C.
In order to introduce an R’ substituent into compounds 3, BOC-protection followed by alkylation and subsequent removal of the BOC group can be performed similarly as described in schemes 2 and 3. Compounds 4 with p = 0 can be prepared from compounds 2 by standard procedures of oxidation of an aromatic aldehyde to an. aromatic acid (e. g with sodium chlorite, sodium dihydrogen-phosphate in a mixture of tert butanol and water and in the presence of 3-methyl-2-butene at temperatures around room temperature). Compounds 4 with p = 1 can be prepared from compounds 2 bya
Wittig reaction using (methoxymethyl)-triphenylphosphonium chloride as reagent, treatment of the Wittig product with acid and oxidation of the aldehyde formed to the corresponding acid. Compounds 4 with p = 2 can be prepared from compounds 2 by a
Horner-Emmons reaction with e. g. dimethyl(benzyloxycarbonyl)methl phosphonate, followed by concomitant reduction of the double bond and liberation of the ester function by e. g. hydrogenation with palladium on charcoal. Condensation of amines 3 or acids 4 with acids 5 or amines 6 can be performed using well known procedures for amid formation, such as use of N-(3-dimethylaminopropyl) -N’-ethyl-carbodiimide- hydrochloride and 4-dimethylamino-pyridine in dichloromethane at temperatures between 0 °C and room temperature yielding compounds Ie (step e) or If (step d).Those can optionally be hydrolyzed according to standard procedures, e. g. by treatment with an alkali hydroxide like LIOH or NaOH in a polar solvent mixture like tetrahydrofuran/ethanol/water, giving carboxylic acids Ie or If.
An analogous reaction scheme with the same reaction sequences applies for the isomeric compound series leading to compounds of general formula, particularly compounds according to formula Ig and Th:
rR®
R® ye=Y 13
R® c 0 _{ rr o —er), J ~ (CH NY 0 7 ~N (CR1OR1) 2/n rR R RSor R? = Ie n [o] ) . rR? R
R™ R
Ig
RS
4 R®
SX J Lr / 1 0] 7 J | NN
Ro ~~ f R" RsorR7= iE CROR™),~ CH y-Y 3
R™ R o :
Th
The synthesis of compounds with the general structure I, particularly compounds according to formula Ii, with X! equal to CH; and X? equal to oxygen can be accomplished according to schemes 5.
Scheme 5 - rR: rE 2 £) i” RS (3 rid
J AN "oY a0 = iN e . , Prot. 2 . , Prot ~~ rR rR
R a [o} R 2 rR o oy 1 3 RY N b c 1 ” H \ \\ / \ co Rr VF
Dai RY R® rR L3 ye a o fo) Rr 3 o Ra Rr? 7 0 r-° Tn ) “Prot. °. r-° “prot. 0 rR" WR o R® FR 5 6 v=Y on
RU RRS An Rt RS
RR SRR, (Fok ey 2 R a
R- H ’ RO ~~ (CHy) Ad o] R® Rr U 7 (CRIR"),,” ° h 0 RR
Aldehydes 1 are known, commercially available or can be prepared by methods known in the art. Aldehydes 1 can be reacted with a Wittig salt 2 such as (1,2-diethoxy-2- oxoethyl)triphenyl phosphonium chloride or (1,2-dimethoxy-2-oxoethyl)triphenyl phosphonium bromide in solvents like isopropanol, dichloromethane or tetrahydrofuran or mixtures thereof in the presence of a base like potassium carbonate, 1,8- diazabicyclo[5.4.0Jundec-7-ene (DBU), 1,1,3,3-tetramethyl-guanidine or sodium tert butylate, preferably between 0 °C and the reflux temperature of the solvents, giving acrylic esters 3 as E and/or Z isomers (step a). Alternatively, a Horner-Emmons reaction can be used for the transformation of compounds 1 into unsaturated esters 3, e. g. using dimethyl (methoxycarbonyl)methl phosphonate, optionally substituted at the methylene group, a base like sodium hydride in a solvent like tetrahydrofuran. Hydrogenation of acrylic esters 3 using palladium on charcoal as catalyst, preferably at room temperature and 1 atm. pressure of hydrogen, in solvents like methanol, ethanol, tetrahydrofuran, acetic acid, dichloromethane and mixtures thereof, affords esters 7, provided that the protecting group can be cleaved reductively (step e).
Alternatively, aldehydes 1 are reacted with the enolate of an acetic acid esters 4 (preferably the lithium-enolate, prepared at —78 °C by treatment of 4 with a strong, non- nucleophilic base like lithium diisopropylamide in an inert solvent like tetrahydrofuran), preferably at temperatures around 78 °C, in solvents like tetrahydrofuran giving the aldol product 5 as a mixture of diasteromers (step b). Removal of the benzylic hydroxy group in compounds 5 can be performed with a reducing agent like e. g. triethylsilane in the presence of a Lewis acid, like boron-trifluoride, or a protic acid, like trifluoroacetic acid, in a suitable solvent like trifluoroacetic acid itself or dichloromethane between 0 °C and 60 °C to yield protected phenol compounds 6 (step d). Subsequent removal of the protecting group, e. g. a benzyl group, by standard technology, e. g. catalytic hydrogenation using hydrogen and a catalyst like palladium or by using dimethyl sulfide and boron trifluoride diethyl etherate in a solvent like dichloromethane between room temperature and the reflux temperature of the solvent gives phenolic compounds 7 (step g). Catalytic hydrogenation can be used to transform unsaturated esters 3 into compounds 6 (step f). In case the protective group in compounds 3 is a benzyl group, then a one step hydrogenation procedure directly gives phenolic compounds 7. Catalytic hydrogenation can also be used for the simultaneous removal of the benzylic hydroxy function and a benzyl protecting group, preferably using palladium on charcoalas ~~~ = catalyst in the presence of an acid like oxalic acid in solvents like alcohols at temperatures around room temperature and a hydrogen pressure up to 100 bar, thus giving the transformation of compounds 5 into compounds 7 in one step (step d and g). As an alternative method, compounds 5 can be treated with catalytic amounts of an acid like para toluene sulfonic acid in a solvent like benzene or toluene, preferably under conditions allowing the removal of the water formed (e. g. with a Dean Stark trap or in the presence of molecular sieves) at temperatures between room temperature and the reflux temperature of the solvents to yield acrylic esters 3 (step ¢). The condensation of phenols 7 with heterocycles 8 to form compounds li can be performed as outlined in scheme l.
An analogous reaction scheme with the same reaction sequences applies for the isomeric compound series leading to compounds of general formula, particularly compounds according to formula Ik:
RR&%, F 1
Y
RR . o by rr = N
RS RSor R7= ~~ Crore, (CHY,~ N\p3-Y! 0 R® FR
Ik
The synthesis of compounds with the general structure I, particularly compounds according to formula II, with X! equal to CH; and X? equal to nitrogen can be accomplished according to schemes 6.
Scheme 6 -
Rr R® v0) Or RS rR . p Or Aad rR P i/ N, - 2° o 1.0 = N, - °o , © 2 R ) , [o)
R R “a [o} R 1 b 3
On rR R RS 4 ,.0 N _ — LO NH,
R H 0 R
[0] R® RY fo) R® oy 5 6 vay, pn
RY s RY rR + 8 } RR Rw one RR " J I SAG r° i 8 R' Pro) & ) Na (CHy), Sy. 5 & wo — [Jw ee 7 n
Nitro-phenyl compounds 3 and 5 are prepared from nitro aldehydes 1, which are known, commercially available or can be prepared by methods known in the art, by
Wittig/Horner-Emmons or aldol reactions analogous to the reactions described for the synthesis of compounds 3 and 5 in scheme 5 (steps a and b). Catalytic hydrogenation can be used for the simultaneous removal of the benzylic hydroxy function (compounds 5) or the reduction of the double bond (compounds 3) and the reduction of the nitro group, preferably using palladium on charcoal as catalyst optionally in the presence ofan acid like oxalic acid in solvents like alcohols at temperatures around room temperature and a hydrogen pressure up to 100 bar (step c). Compounds 7 with R’ substituents different from hydrogen are obtained by first introduction of a BOC group, alkylation and removal of the BOC protective function as described in schemes 2 and 3. The condensation of anilines 7 with heterocycles 8 to form compounds Il can be performed as outlined in scheme 3.
An analogous reaction scheme with the same reaction sequences applies for the isomeric compound series leading to compounds of general formula I, particularly compounds according to formula Im: rR FR
Rr! 0 or RY RoR, (CH, NY VY ) R® R
Im
As alternative to the sequences described in scheme 6, the nitrogen containing intermediates can be prepared from suitable intermediates carrying a phenolic hydroxyl function. In such intermediates, optionally carrying one or more protective functions, the phenolic OH group can be replaced by the corresponding aromatic NH; function by methods known in the art. For example by a three step sequence as described in
Tetrahedron Letters 43(42), 7617-7619 (2002) and discussed in the context of schemes 2 and 3.
The synthesis of compounds compounds with the general structure I, particularly : - --- compounds according to formula In and Io, with X! equal to CH, and X2 equal to - - (CH,),NR’CO, or (CHa),CONR’ can be accomplished according to schemes 7.
Scheme 7
RRR
2 a oF RY Rr? o” PS yg 12 Od R oH 4 _- —
RS 8 R 2 [¢] rR — 7 gt HOR a lo} Rr WR ® Rb °/ 3 b
NP
4 JO CN/CHO
R H
0 RR Rs 3 i oe I I
RY b RY (Ck,), OH rR R Ri R [¢} " rR o) os rR 6 7 1
YY RY
YY gw \ CH A he 1 1 YR RY ERR), Dn YS
HO crim,” OH 8 v-Y t 9
RE ¥ en
C N fied on auras
R! RO ~0 As py In ve=Y. 13
R rR a Oy mn. oS 9
RA RN
Oo C]
Rr? rR R Io
Formyl-nitriles 1a or aldehydes 1b, carrying a protected aldehyde function, are known, commercially available or can be prepared by methods known in the art. 5 Compounds 3 and 5 are prepared from aldehydes 1 by Wittig/Horner-Emmons or aldol reactions analogous to the reactions described for the synthesis of compounds 3 and 5 in scheme 5 followed by removal of the aldehyde acetal protective function (if applicable) (steps a and b). Compounds 5 can be transformed into compounds 3 by treatement with catalytic amounts of an acid like para toluene sulfonic acid in a solvent like benzene or toluene, preferably under conditions allowing the removal of the water formed (e. g. with a Dean Stark trap or in the presence of molecular sieves) at temperatures between room temperature and the reflux temperature of the solvents to yield acrylic esters 3 (step c).
Acrylic esters 3 carrying an aldehyde or a nitrile function can be further transformed into compounds of the general formula 6 or 7 using selective transformations as outlined for the transformation of nitrile 1 (scheme 4) and aldehyde 2 (scheme 4). Subsequent amide formation with heterocycles 5 and 6 can also be performed as outlined in scheme 4 thus yielding compound of the general formula In and Io as esters or acids.
An analogous reaction scheme with the same reaction sequences applies for the isomeric compound series leading to compounds of general formula, particularly compounds according to formula Ip and Iq:
RE r® vey. R®
RY rR® —(CH,) I} em 1 0 RSor R= T {CRYOR),, y 0)
Ro f R
RY R* R
Ip
R® 1 v=Y 13
RY R’ fo YR
Rr! 3 IX rR Réor R7= “eH erm” nN ~ . 9 © rR R* a R q
The synthesis of compounds with the general structure I, particularly compounds with X! and / or X* equal to S can be accomplished in close analogy to the synthesis of the corresponding analogues with X' and / or X’ equal to oxygen. Suitable sulfur containing intermediates are known, can be prepared by methods known in the art or are prepared from phenolic intermediates as described by W Zhi-Liang and AP Kozikowski (J. Org.
Chem. 2003, web publication release Oct. 10, 2003): treatment of a phenolic intermediate with sodium thiocyanate, sodium bromide and bromine in a solvent like methanol preferably between 0 °C and room temperature gives the corresponding 4-thiocyanato- phenols; subsequent reduction with lithiumaluminium hydride in a solvent like : tetrahydrofuran at temperatures around 0 °C then liberates the corresponding 4- mercapto-phenol. Alternatively, intermediates carrying an aromatic SH moiety can be prepared from suitable intermediates carrying a phenolic hydroxyl function. In such intermediates, optionally carrying one or more protective functions, the phenolic OH group can be replaced by the corresponding aromatic SH function by methods known in the art. For example by a three step sequence as described in J. Labelled Compounds &
Radiopharmaceuticals 43(7), 683-691, (2000): i) transformation of the phenol moiety into its trifluoromethanesulfonate (triflic anhydride, triethylamine, dichloromethane, at low temperature, preferably around -30 °C); ii) treatment of the triflate with triisopropylsilanethiolate, tetrakis(triphenylphosphine)-palladium(0) in solvent mixtures like toluene and tetrahydrofuran in a temperature range between 60 °C and 150 °C; iii) treatment of the silyl sulfide with hydrogen chloride in methanol preferably around 0 °C to liberate the phenolic SH moiety.
Compounds of the general formula I may be obtained in the form of racemates.
Racemic compounds can be separated into their antipodes by methods known in the art, such as separation of the antipodes via diastereomeric salts by crystallization with optically pure amines such as e. g- (R) or (S)-1-phenyl-ethylamine, (R) or (S)-1- naphthalen-1-yl-ethylamine, brucine, quinine or quinidine or by separation of the antipodes by specific chromatographic methods using either a chiral adsorbens or a chiral eluent.
Schemes 8 to 11 describe the synthesis of heterocycles 5 (scheme 1), identical to 11 (scheme 3), 8 (scheme 5) and 8 (scheme 6); 5 (scheme 4) identical to 8 (scheme 7) and 6 (scheme 4) identical to 9 (scheme 7).
Scheme 8
L 2 NHMe,# Ci” b i Allgt ag + Ne, HCl + parstomaldetyte —— ne —_— ® 0 . \ RZ ry A : | 4
Hy R™ yd [4 ad R™ RY Q e
R' ZN] R 7 ~R? IN 7 “R® 7 6 [] -- Pyridines 5 and 7 can be synthesized in a three step synthesis from ketones 1 (scheme 8). A mixture of ketones 1 with paraformaldehyde and dimethylamine hydrochloride in a solvent like ethanol in the presence of an acid like 37% HCl is heated to reflux for 2 to 10 hours to give aminoketones 2 (step a). Reaction of compounds 2 with 3-aminocrotonic acid esters 3 in acetic acid at reflux for 2 to 8 hours gives esters 4 (step b), which can be hydrolyzed (alkali hydroxide in solvents like THF, dioxane or
DMSO) to give acids 5 (step c). Alternatively, esters 4 can be reduced with diisbutylaluminium hydride-solution (in toluene) at -30 °C to room temperature for 30 min to 3h in solvents like THF to give alcohols 6. Reaction of alcohols 6 with thionyl chloride in dichloromethane at 0 °C to room temperature for 5 min to 1h gives access to chlorides 7. Pyridines 4 can alternatively be synthesized following procedures described in Al-Saleh, Balkis; Abdelkhalik, Mervat Mohammed; Eltoukhy, Afaf Mohammed;
Elnagdi, Mohammed Hilmy (Enaminones in heterocyclic synthesis: A new regioselective synthesis of 2,3,6-trisubstituted pyridines, 6-substituted-3-aroylpyridines and 1,3,5- triaroylbenzenes. Journal of Heterocyclic Chemistry (2002), 39(5), 1035-1038).
Disubstituted pyridines 4 can be prepared according to procedures described in
Katsuyama, Isamu; Ogawa, Seiya; Yamaguchi, Yoshihiro; Funabiki, Kazumasa; Matsui,
Masaki; Muramatsu, Hiroshige; Shibata, Katsuyoshi (A convenient and regioselective synthesis of 4-(trifluoromethyl)pyridines. Synthesis (1997), (11), 1321-1324).
Scheme 9 o ©
SOG
0% ~0-alkyi b
IN n , pra e N=( © a : ) Dat ) = {oa oS _— © ey © : Ho R* rR"? 4 5 3 [:} 1 ed R d 13 N= ! N=
RE Dan — RE Da
N ci N OH
R* RZ
RY
8 7 n= © {7
N OH
R"™
The synthesis of chiormethyl pyrimidines 8 and pyrimidine acids 6 is described in scheme 9. Reaction of 3-oxo-esters 1 with triethyl orthoformate in acetic anhydride at room temperature fo reflux for 1 to 8 hours gives an E/Z mixture of the 3-ethoxy-acrylic acid esters 3 (step a). Diketo-esters 2 are reacted with methyl triflate in the presence of cesium carbonate in acetonitrile to give O-methylated products 3 (step b) [S. W.
McCombie et al., Bioorganic & Medicinal Chemistry Letters 13 (2003), 567-571}, thus yielding substituted enolethers 3 (RZ not H). Reaction with amidine hydrochlorides 4 in ethanol in the presence of alkali tert-butoxide at room temperature gives access to esters 5 (step c). Bsters 5 can be hydrolyzed (alkali hydroxide in solvents like THF, dioxane or
DMSO) to give acids 6 (step d). Alternatively, esters 5 can be reduced with diisbutyl- aluminium hydride-solution (in toluene) at -30 °C to room temperature for 30 min to 3h in solvents like THF to give alcohols 7 (step e). Reaction of alcohols 7 with thionyl chloride in dichloromethane at 0 °C to room temperature for 5 min to 1h gives access to chlorides 8 (step f).
Scheme 10 ‘0 O
OH a
WB + PN Soa — A oa on Mw Av rR? all rR"? all 1 2 3 /
Y} 1 So © vi Sy on
A oY ys -Y b
R Y R Y
7 6 fH oO y on ww Cp PA on
Ar pW 5 4
A general synthesis for acids 4 and chlorides 6 is depicted in scheme 10. Suzuki- coupling with boronic acides 1 and 6-halo-pyridazine-3-carboxylic acid esters 2, 5-halo- pyrazine-2-carboxylic acid esters 2, 6-halo-nicotinic acid esters 2, 5-halo-pyridine-2- carboxylic acid esters 2, 2-halo-pyrimidine-5-carboxylic acid esters 2 or 5-halo- pyrimidine-2-carboxylic acid esters 2 with Pd(PhP), or PAC (dppf) [(L,1'- bis(diphenylphosphino)ferrocene)-dichloropalladium(IxCH;Cl, (1:1)] in toluene, dimethoxyethane, ethanol or DMF with cesium carbonate, potassium carbonate or "cesium fluoride at room temperature to 90 °C for 2 to 8 h give esters 3 (step a). Esters 2 are either commercially available or can be prepared by methods known to a person skilled in the art. Esters 3 can be hydrolyzed (alkali hydroxide in solvents like THF, dioxane or DMSO) to give acids 4 (step b). A Curtius rearrangement can be used to transform acids 4 into the analogous BOC-protected aniline: first, the acid chlorides are synthesized with e.g. oxalyl chloride/DMF in dichloromethane. Then, reaction with sodium azide in DMF/ dichloromethane followed by heating to reflux in the presence of 2-methyl-2-propanol gives the BOC protected anilines. Alternatively, such BOC protected anilines can be obtained from acids 4 in a one pot procedure by treatment with diphenylphosphoryl azide in 2-methyl-2-propanol in the presence of triethylamine and anhydrous 4-toluene sulfonic acid at temperatuares arount 100 °C. Alkylation of these
BOC protected anilines with R’-halide in the presence of sodium hydride in solvents like
DMEF followed by BOC-deprotection with TFA or HCl in dioxane yields anilines 5 (step ©). Alternatively, esters 3 can be reduced with diisbutylaluminium hydride-solution (in toluene) at -30 °C to room temperature for 30 min to 3 h in solvents like THF to give alcohols 6 (step d). Reaction of alcohols 6 with thionyl chloride in dichloromethane at 0 °C to room temperature for 5 min to 1h gives access to chlorides 7 (step e).
Scheme 11
Y2 a Y: b
Yi Spt on vi St He AY — . YS SN oA Av or Ae 1 2 3
Ng EEN
ARS SAH? heen o , v2 RY rR" n 0 " | 8 A R Ae? 6 4 d 4 ‘
Y3 R™ h 2
Yi [> ry Pe OH Y
THe 2 0 re rR" Y R™ v" R A R' RY 7, RY = leaving group 5 9
Alcohols 1 in scheme 11 comprising a chain length n equal to one or two can be converted into analogues with a chain length of n+1 carbon atoms by methods well known in the art, e. g. by conversion of the primary alcohol into a suitable leaving group, e.g a halide (2, step a), followed by reaction with cyanide to form nitriles 3 (step b) and saponification to acids 4 (step c). Acids 4 can be further transformed into the primary alcohols 5 (R= H, R'! = H), e. g. by using diborane in tetrahydrofuran (step d).
Optionally, such alcohols 5 can be elongated to a chain length of n++1 carbon atoms by repeating the synthesis described for alcohols 1 to 5. In order to introduce substituents
R'° and/or R! different from hydrogen, cyano intermediates 3 can be reacted with alkyl
Grignard reagents R\°MgX in solvents like ether or tetrahydrofuran between 0 °C and then reflux temperature of the solvent to form the corresponding R¥CO-alkyl ketones 6 (step e) or with diisbutylaluminium hydride the corresponding aldehydes 6 (R"=H).
Treatment of compounds 6 with an alkyllithium reagent R''Li in solvents like ether or tetrahydrofuran gives alcohols 5 (step f); treatment of compounds 6 with lithium . aluminium hydride in solvents like tetrahydrofuran or ether or with sodium borohydride ... in solvents like ethanol or methanol, preferably at temperatures between —15 °C and 40 °C, gives alcohols 5 with R"=H (step f). The alcohol compounds 5 which contain a chiral center can optionally be separated into optically pure antipodes by methods well known in the art, e. g. chromatography on a chiral HPLC column, or by derivatization with an _ optically pure acid to form esters, which can be separated by conventional HPLC chromatography and can then be converted back to the enantiomericaly pure alcohols 5.
The reduction of ketones 6 to the corresponding secondary alcohols 5 of scheme 11 can also be carried out in an enantioselective fashion leading to the (R)- or (S)-alcohols 5, e g. by treatment with borane-dimethylsulfide complex and (S)- or (R)-2-methyl-CBS- oxazaborolidine as chiral catalyst in tetrahydrofuran, preferably at temperatures between —78 °C and ambient temperature, according to Corey et al. (E. J. Corey, R. K. Bakshi, S.
Shibata, J. Am. Chem. Soc. 1987, 109, 5551-5553), or by treatment with (+)- or (-)-B- chlorodiisopinocampheyl-borane (DIP-Cl), according to Brown et al. (P. V.
Ramachandran, B. Gong, A. V. Teodorovic, H. C. Brown, Tetrahedron: Asymmetry 1994, 5, 1061-1074). Aldehydes 6 (R'=H, n = 0) can also be synthesized from primary alcohols 1 by methods known in the art, e. g. by treatment with pyridinium chlorochromate in dichloromethane, preferably at temperatures between room temperature and the reflux temperature of dichloromethane, or by treatment with manganese dioxide in solvents like dichloromethane, preferably at room temperature (step g). These aldehydes 6 can be converted to the corresponding secondary alcohols 5 through reaction with alkyl organometallic compounds, preferably under the conditions discussed above. Finally, the alcohols 5 of scheme 11 can be converted into compounds of formula 7, e. g by treatment with methanesulfonyl chloride in dichloromethane in the presence of a base like triethylamine preferably in a temperature range between —20 °C and room temperature or thionyl chloride in dichloromethane at 0 °C to room temperature or by reaction with carbon tetrachloride or carbon tetrabromide and triphenylpbosphine in solvents like tetrahydrofuran preferably in a temperature range between room temperature and the reflux temperature of the solvents or by treatment with triflic anhydride, 2,6-lutidine and 4-dimethylaminopyridine in dichloromethane between -30 °C and room temperature; thus yielding compounds of formula 7 as methane-sulfonates, triflates, chlorides or bromides, respectively (step h). Compounds of formula 7 can further be converted (reaction step i) to the amines 8 in solvents like
DMA, DME or dichloromethane at room temperature with an excess of the corresponding amine.
Alpha mono- or di-substituted acids 9 (R' and/or R"* not H) can be synthesized via esters of compounds 4, by treatment with a base like LDA or HMDS in solvents like tetrahydrofuran or 1,2-dimethoxyethane, followed by addition of one or sequentially two ~ different alkyl halides, a reaction preferably performed between -78 °Candroom ~~ temperature followed by hydrolysis to acid 9 (step k). Compounds 9 can be chiral and can optionally be separated into optically pure antipodes by methods well known in the art, e. g. chromatography on a chiral HPLC column, or by derivatization with an optically pure acid to form esters, which can be separated by conventional HPLC chromatography and then converted back to the enantiomerically pure alcohol.
Additionally, the asymmetric alkylation can be done with chiral amides of 4 which are well known to a person skilled in the art.
The synthesis of compounds with the general structure I, particularly compounds according to formula Ir (scheme 12), with X! and / or X?equal to S can be accomplished in close analogy to the synthesis of the corresponding analogues with X! and / or X? equal to oxygen and is generally described on pages 41 to 42. In scheme 12, a general synthesis for X! equal to O and X* equal to S is shown.
Scheme 12
RA, Hatide or Trifiste er
R® rR 3 R®
R seN [ge s— b R® s— 7 O
HO R HO rR 1 0 Rr
Rr? s Ro 8
R 2 rR? rR’ R 2 1 2 4 ‘
R®
RY Ssps : rR Le R y=Y. RY [o) 8
RY bal 2 s R trom, a WY § ) 5 6 _ rR? v= R®
RY $s N lg
ERR, - oN
O rR
Ro rR : ATS a oo
Ir
Thiocyanato phenols 1 which are known or can be prepared as discussed before (page 41-42) can be hydrolyzed with NaOH in water at room temperature to reflux temperature and oxidized in DMSO at higher temperature, preferably around 95 °C to the dithiols 2 (step a). Thiophenols 1 described before can be oridized to the dithiols 2 as well (step a). Reaction of phenols 2 with alpha halo esters ortriflates of formula 3 in the presence of a base like potassium or cesium carbonate in solvents like acetone, methyl- ethyl ketone, acetonitrile or N,N-dimethylformamide in a temperature range between room temperatureand 100 °C leads to the corresponding ether compounds 4 (step b).
Dithiol reduction with (n-BusPH)BF, and Hiinig's base in DMF at room temperature as described in the literature Netherton, M. R.; Fu, G. C. Organic Letters 2001, 3(26), 4295-4298] yields thiophenols 5 (R = H) (step c). The reaction can also be carried out with acetic acid anhydride to give the stable compounds 5 (R® = Ac) (step ©).
Heterocycles 6 (prepared as outlined in schemes 8 to 11) are condensed with thiophenols 5 (R® = H) according to well known procedures (step d): if R® represents a hydroxy group e. g. via Mitsunobu-reaction, with triphenylphosphine and di-tert-butyl-, diisopropyl- or diethyl-azodicarboxylate as reagents; this transformation is preferably carried out in a solvent like toluene, dichloromethane or tetrahydrofuran at ambient temperature. Alternatively, if R* represents a halide, mesylate or tosylate, heterocycles 6 can be reacted with S-acetyl protected 5 (R® = Ac) in solvents like N,N- dimethylformamide, dimethylsulfoxide, acetonitrile, acetone or methyl-ethyl ketone in the presence of methanol and a weak base like cesium or potassium carbonate at a temperature ranging from 0 °C to 140 °C, preferably around room temperature to yield ether compounds Ir (step d). Heterocycles 6 with R!*=OH can also be transformed in situ to the coresponding triflates by treatment with trifluoromethanesulfonic anhydride/2,6- di-tert-butylpyridine in CH,Cl, at 0 °C to room temperature. The triflates are then reacted with thiophenols 5 (R*® = H) in solvents like N,N-dimethylformamide, dimethylsulfoxide, acetonitrile, acetone or methyl-ethyl ketone in the presence of a weak base like cesium or potassium carbonate at a temperature ranging from room temperature to 140 °C, preferably around 50 °C to yield ether compounds Ir (step d).
Esters of formula Ir can optionally be hydrolyzed according to standard procedures, e. g. by treatment with an alkali hydroxide like LiOH or NaOH in a polar solvent mixture like tetrahydrofuran/ethanol/water leading to carboxylic acids Ir.
An analogous reaction scheme with the same reaction sequence applies for the isomeric compound series leading to compounds of general formula I, particularly compounds according to formula Is:
R® 1
R: rR 3 re
J Rsor RT= ~~ _ CHD NY 0 o R (CR®©R"),,
RL oA R®
RY R
Is
Compounds of the general formula I with X! = S can be prepared from the appropriate thiophenol precursors which themselves can be obtained by converting suitable phenol intermediates into the corresponding thiophenols applying methods 5s described above.
Compounds of the general formula I can contain one or more stereocenters and can optionally be separated into optically pure enantiomers or diastereomers by methods well known in the art, e. g. by HPLC chromatography, chromatography on a chiral HPLC column, chromatography with a chiral eluant or by derivatization with an optically pure alcohol to form esters, which can be separated by conventional HPLC chromatography and then converted back to the enantiomerically pure acids I (R! = H). In addition, racemic compounds can be separated into their antipodes via diastereomeric salts by crystallization with optically pure amines such as e. g. (R) or (S)-1-phenyl-ethylamine, (R) or (S)-1-naphthalen-1-yl-ethylamine, brucine, quinine or quinidine.
The following tests were carried out in order to determine the activity of the compounds of formula (I).
Background information on the performed assays can be found in: Nichols JS et al. “Development of a scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain”, (1998) Anal. Biochem. 257: 112-119.
Full-length cDNA clones for humans PPARS and PPAR and mouse PPARY were obtained by RT-PCR from human adipose and mouse liver cRNA, respectively, cloned into plasmid vectors and verified by DNA sequencing. Bacterial and mammalian expression vectors were constructed to produce glutathione-s-transferase (GST) and Gal4
DNA binding domain proteins fused to the ligand binding domains (LBD) of PPARS (aa oo 139 to 442), PPARy (aa 174 to 476) and PPAR« (aa 167 to 469). To accomplish this, the portions of the cloned sequences encoding the LBDs were amplified from the full-length clones by PCR and then subcloned into the plasmid vectors. Final clones were verified by
DNA sequence analysis.
Induction, expression, and purification of GST-LBD fusion proteins were performed in E. coli strain BL21(pLysS) cells by standard methods (Ref: Current
Protocols in Molecular Biology, Wiley Press, edited by Ausubel et al.).
Radioligand Binding Assay
PPARS receptor binding was assayed in HNM10 (50mM Hepes, pH 7.4, 10 mM
NaCl, 5mM MgCl, 0.15 mg/ml fatty acid-free BSA and 15 mM DTT). For each 96 well reaction a 500 ng equivalent of GST-PPARS-LBD fusion protein and radioligand, e.g. 20000 dpm {2-methyl-4-[4-methyl-2-(4-trifftuoromethyl-phenyl)-thiazol-5-y- ditritiomethylsulfanyl}-phenoxy}-acetic acid, was bound to 10 pg SPA beads (PharmaciaAmersham) in a final volume of 50 pl by shaking. The resulting slurry was incubated for 1h at RT and centrifuged for 2 min at 1300g. The supernatant containing unbound protein was removed and the semidry pellet containing the receptor-coated beads was resuspended in 50 ul of HNM. Radioligand was added and the reaction incubated at RT for 1h and scintillation proximity counting performed in the presence of test compounds was determined. All binding assays were performed in 96 well plates and the amount of bound ligand was measured on a Packard TopCount using OptiPlates (Packard). Dose response curves were done in triplicates within a range of concentration from 10° M to 10 M.
PPARa receptor binding was assayed in TKES0 (50mM Tris-HCl, pH 8, 50 mM
KCl, 2mM EDTA, 0.1 mg/ml fatty acid-free BSA and 10 mM DTT). For each 96 well reaction an 140 ng equivalent of GST-PPARa-LBD fusion protein was bound to 10 ug
SPA beads (PharmaciaAmersham) in a final volume of 50 ul by shaking. The resulting slurry was incubated for 1h at RT and centrifuged for 2 min at 1300g. The supernatant containing unbound protein was removed and the semidry pellet containig the recptor- 5 coated beads was resolved in 50 pl of TKE. For radioligand binding e.g. 10000 dpm of 2(8)-(2-benzoyl-phenylamino)-3-{4-[1,1-ditritio-2-(5-methyl-2-phenyl-oxazol-4-yl)- ethoxy)-phenyl}-propionic acid or 2,3-ditritio-2(S)-methoxy-3-{4-[2-(5-methyl-2- phenyl-oxazol-4-yl)-ethoxy}-benzo [b]thiophen-7-yl}-propionic acid in 50 ul were added, the reaction incubated at RT for 1h and scintillation proximity counting performed. All binding assays were performed in 96 well plates and the amount of bound ligand measured on a Packard TopCount using OptiPlates (Packard). Nonspecific binding was determined in the presence of 10° M unlabelled compound. Dose response curves were done in triplicates within a range of concentration from 107° M to 10 M.
PPARY receptor binding was assayed in TKE50 (50mM Tris-HCl, pH 8, 50 mM
KCl, 2mM EDTA, 0.1 mg/ml fatty acid-free BSA and 10 mM DTT). For each 96 well reaction an 140 ng equivalent of GST-PPARy-LBD fusion protein was bound to 10 pg
SPA beads (PharmaciaAmersham) in a final volume of 50 ul by shaking. The resulting slurry was incubated for 1h at RT and centrifuged for 2 min at 1300g. The supernatant containing unbound protein was removed and the semidry pellet containig the recptor- coated beads was resolved in 50 ul of TKE. For radioligand binding e.g. 10000 dpm 2(S)- (2-benzoyl-phenylamino)-3-{4-[1,1-ditritio-2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxyl- phenyl}-propionicacid in 50 pl were added, the reaction incubated at RT for 1h and scintillation proximity counting performed. All binding assays were performed in 96 well plates and the amount of bound ligand measured on a Packard TopCount using
OptiPlates (Packard). Nonspecific binding was determined in the presence of 10*M unlabelled compound. Dose response curves were done in triplicates within a range of concentration from 10° M to 10 M.
Luciferase Transcriptional Reporter Gene Assays
Baby hamster kidney cells (BHK21 ATCC CCL10) were grown in DMEM medium containing 10% EBS at 37 °C in a 95%02:5%CO; atmosphere. Cells were seeded in 6 well plates at a density of 10° Cells/well and then batch-transfected with either the pFA-
PPARS-LBD, pFA-PPARy-LBD or pFA-PPARa-LBD expression plasmids plus a reporter plasmid. Transfection was accomplished with the Fugene 6 reagent (Roche Molecular
Biochemicals) according to the suggested protocol. Six hours following transfection, the cells were harvested by trypsinization and seeded in 96 well plates at a density of 10* cells/well. After 24 hours to allow attachment of cells, the medium was removed and replaced with 100 ul of phenol red-free medium containing the test substances or control ligands (final DMSO concentration: 0.1%). Following incubation of the cells for 24 hours with substances, 50 pl of the supernatant was was discarded and then 50 ul of Luciferase
Constant-Light Reagent (Roche Molecular Biochemicals) to lyse the cells and initiate the luciferase reaction was added. Luminescence for luciferase was measured in a Packard
TopCount. Transcriptional activation in the presence of a test substance was expressed as fold-activation over cells incubated in the absence of the substance. EC50 values were calculated using the XLfit program (ID Business Solutions Ltd. UK).
The free acids of the compounds of the present invention (R! is hydrogen) exhibit
ICs values of 0.5 nM to 10 uM, preferably 1 nM to 100 nM for PPARS and ICs; values of __1nMto 10 uM, preferably 10 nM to 5 pM for PPARc. Compounds, in which R'is not hydrogen are converted in vivo to compounds in which R' is hydrogen. The following table shows measured values for some selected compounds of the present invention.
PPAR PPARY PPARS
ICso (pmol/l) | ICso (nmol) | ICso (pmol/l)
The compounds of formula (I) and their pharmaceutically acceptable salts and esters can be used as medicaments, e.g. in the form of pharmaceutical preparations for enteral, parenteral or topical administration. They can be administered, for example, perorally, e.g. in the form of tablets, coated tablets, dragées, hard and soft gelatine capsules, solutions, emulsions or suspensions, rectally, e.g. in the form of suppositories, parenterally, e.g. in the form of injection solutions or infusion solutions, or topically, e.g. in the form of ointments, creams or oils.
The production of the pharmaceutical preparations can be effected in a manner which will be familiar to any person skilled in the art by bringing the described compounds of formula (I) and their pharmaceutically acceptable, into a galenical administration form together with suitable, non-toxic, inert, therapeutically compatible solid or liquid carrier materials and, if desired, usual pharmaceutical adjuvants.
Suitable carrier materials are not only inorganic carrier materials, but also organic carrier materials. Thus, for example, lactose, corn starch or derivatives thereof, talc, stearic acid or its salts can be used as carrier materials for tablets, coated tablets, dragées and hard gelatine capsules. Suitable carrier materials for soft gelatine capsules are, for example, vegetable oils, waxes, fats and semi-solid and liquid polyols (depending on the nature of the active ingredient no carriers are, however, required in the case of soft gelatine capsules). Suitable carrier materials for the production of solutions and syrups are, for example, water, polyols, sucrose, invert sugar and the like. Suitable carrier materials for injection solutions are, for example, water, alcohols, polyols, glycerol and © vegetable oils, Suitable carrier materials for suppositories are, for example, natural or hardened oils, waxes, fats and semi-liquid or liquid polyols. Suitable carrier materials for topical preparations are glycerides, semi-synthetic and synthetic glycerides, hydrogenated oils, liquid waxes, liquid paraffins, liquid fatty alcohols, sterols, polyethylene glycols and cellulose derivatives.
Usual stabilizers, preservatives, wetting and emulsifying agents, consistency- improving agents, flavour-improving agents, salts for varying the osmotic pressure, buffer substances, solubilizers, colorants and masking agents and antioxidants come into consideration as pharmaceutical adjuvants.
The dosage of the compounds of formula (I) can vary within wide limits depending on the disease to be controlled, the age and the individual condition of the patient and the mode of administration, and will, of course, be fitted to the individual requirements in each particular case. For adult patients a daily dosage of about 1 mg to about 1000 mg, especially about 1 mg to about 100 mg, comes into consideration. Depending on the dosage it is convenient to administer the daily dosage in several dosage units.
The pharmaceutical preparations conveniently contain about 0.1-500 mg, preferably 0.5-100 mg, of a compound of formula (I).
The following examples serve to illustrate the present invention in more detail.
They are, however, not intended to limit its scope in any manner.
Abbreviations:
AcOEt = ethyl acetate, n-BuLi = n-butyllithium, DBU = 1,8-diazabicyclo[5.4.0]undec-7- ene, DEAD = diethyl azodicarboxylate, DIAD = diisopropyl azodicarboxylate, DIBAL-H solution = diisobutylaluminum hydride solution, DMF = N,N-dimethylformamide,
DMPU = 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone, eq. = equivalents, h = hour(s), DMSO = dimethyl sulfoxide, HPLC = high performance liquid chromatography, i. V. = in vacuo, LDA = lithium diisopropylamide, PdCl(dppf) = (1,1'- bis(diphenylphosphino)ferrocene)dichloro-palladium(Il).CHzCl, (1:1), Pd(PhsP)s = tetrakis(triphenylphosphine)palladium, POC]; = phosphorous oxychloride, RT = room temperature, TFA = trifluoroacetic acid, TFAA = trifluoroacetic anhydride, THF = tetrahydrofuran.
Example 1 (2-(3-Methoxy-propyl)-4-{methyl- [2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ylmethyl]-amino}-phenoxy)-acetic acid
Al (2-Iodo-phenoxy)-acetic acid ethyl ester 9.10 g (41.4 mmol) of o-iodophenol was dissolved in 102 ml of acetone and treated subsequently at 0 °C with 14.8 g (1.1 eq.) of cesium carbonate and 4.57 ml (1.0 eq.) of ethyl bromoacetate. After vigorous stirring for 1 h at ambient temperature and filtration, the bulk of solvent was evaporated and the residue redissolved in AOE. Washing with water, drying over magnesium sulfate, and evaporation of the solvents finally produced 12.66 g of pure title compound as colorless oil.
MS: 306.0 (M)*.
B] [2-(3-Methoxy-prop-1-ynyl)-phenoxyl-acetic acid ethyl ester
A three neck reaction flask was successively charged with 12.65 g (41.3 mmol) of the above prepared (2-iodo-phenoxy)-acetic acid ethyl ester, 128 ml of acetonitrile, 5.793 g _ (2eq) of 3-methoxy-1-propyne, 17.28 ml (3 eq.) of NEts, 145g (0.05 eq.) of (PPh;),PdCl,, and 0.394 g (0.05 eq.) of Cul. After stirring for 4 h the bulk of the solvent was removed i. V. and the residue distributed between HCl and AcOEt. Washing of the organic layer with cold water and brine, drying over magnesium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO, hexane/AcOEt=8/2), yielded 9.54 g of the title compound as light brown oil.
MS: 248.2 (M)".
C] [2-(3-Methoxy-propyl)-phenoxy}-acetic acid ethyl ester 9.54 g (38.4 mmol) of the above prepared [2-(3-methoxy-prop-1-ynyl)-phenoxy] -acetic acid ethyl ester was hydrogenated at RT in 130 ml of AcOEt over 2.39 g of Pd/C (10%) under 1 atm of Hy-pressure. After 2 h at ambient temperature, the reaction mixture was fltrated over Celite and carefully rinsed with AcOEt. Evaporation of the solvent left finally 9.39 g of the title compound as colorless oil.
MS: 252.2 (M)".
D] [2-(3-Methoxy-propyl)-4-nitro-phenoxy]-acetic acid ethyl ester 4.00 g (15.9 mmol) of the above prepared {2- (3-methoxy-propyl)-phenoxy}-acetic acid ethyl ester was dissolved in 5.0 ml of TFAA and added slowly and carefully via dropping funnel to a mixture of 5 ml of TFAA and 4 ml! of conc. HNO; (65%) kept at -10 °C; the reaction was then allowed to proceed for additional 30 Min. Careful quenching with ice, twofold extraction with AcOEt, washing with NaHCO; and brine, drying over magnesium sulfate, and evaporation of the solvents left a crude product which was purified by flash chromatography (SiO2, hexane/AcOEt=75/25) to deliver finally 3.00 g of the title compound as light brown oil, contaminated according to NMR with roughly 359% of the ortho-isomer which was separated after the next step.
MS: 298.3 (M+H)".
El] [4-Amino-2-(3-methoxy-propyl)-phenoxy]-acetic acid ethyl ester 3.00 g (roughly 65%, 6.56 mmol) of the above prepared [2-(3-methoxy-propyl)-4-nitro- phenoxy]-acetic acid ethyl ester was hydrogenated at RT in 50 ml of AcOEt over 0.60 g of
Pd/C (10%) under 1 atm of Hz-pressure. After 3 h at ambient temperature, the reaction mixture was filtered over Celite and carefully rinsed with AcOEt. Evaporation of the solvent, followed by flash chromatography (SiOx, hexane/AcOEt=1/1) produced 1.69 g of the title compound as light brown oil; from the more polar fractions, 0.784 g of 8-(3- methoxy-propyl)-4H-benzo| 1,4] oxazin-3-one was isolated (cyclized reduced ortho- _ . amino derivative).
MS: 268.3 (M+H)"; side product: 221.1 (M)".
F] [4-tert-Butoxycarbonylamino-2-(3-methoxy-propyl)-phenoxy]-acetic acid ethyl ester 1.68 g (6.28 mmol) of the above prepared [4-amino-2-(3-methoxy-propyl)-phenoxy]- acetic acid ethyl ester was dissolved in 7 ml of THF, treated with 1.646 g (1.2 eq.) of di- tert-butyl dicarbonate, and refluxed for 1 h. After cooling, the reaction mixture was poured onto crashed ice/AcOEt, the organic layer washed with water and brine, dried over magnesium sulfate, and evaporated to dryness. Flash chromatography (SiO, hexane/AcOEt=75/25) afforded 2.22 g of the title compound as light brown oil.
MS: 387.2 (M)*.
G1 [4-(tert-Butoxycarbonyl-methyl-amino)-2-(3-methoxy-propyl)-phenoxy] -acetic acid ethyl ester
To 2.21 g (6.01 mmol) of the above prepared [4-tert-butoxycarbonylamino-2-(3- methoxy-propyl)-phenoxy]-acetic acid ethyl ester, dissolved in 18 ml of abs. DMF, was added at 0 °C 0.313 g of NaH (60% in mineral oil, 1.3 eq.). 5 Min. later, 0.75 ml (2 eq.) of
Mel was added and the reaction allowed to proceed for 10 Min. at 0 °C and for 1 h at ambient temperature. Pouring onto crashed ice/KHSO, solution, twofold extraction with
AcOEt, washing with water and brine, drying over magnesium sulfate, and evaporation of the solvents, followed by flash chromatography (SiOz, hexane/AcOEt = 8/2), yielded 1.55 g of the title compound as light yellow oil.
H] [2-(3-Methoxy-propyl)-4-methylamino-phenoxy] -acetic acid ethyl ester 1.55 g (4.06 mmol) of the above prepared [4-(tert-butoxycarbonyl-methyl-amino)-2-(3- methoxy-propyl)-phenoxy]-acetic acid ethyl ester was dissolved in 41 ml of CHCl and treated dropwise with 10 ml of TFA. After additional 30 Min. at RT, the bulk of the solvents was removed i. V. and the residue distributed between cold NaHCO;-solution and AcOEt. Washing with cold water and brine, drying over magnesium sulfate, and evaporation of the solvents left 1.15 g of the title compound as brownish oil which was used as such for the next step.
I] (2-(3-Methoxy-propyl)-4-{methyl-[2-methyl-6-( 4-trifluoromethyl-phenyl)-pyridin-3- ylmethyl]-amino}-phenoxy)-acetic acid ethyl ester
To 0.102 g (0.36 mmol) of the above prepared [2-(3-methoxy-propyl)-4-methylamino- phenoxy]-acetic acid ethyl ester, dissolved in 1.4 ml of abs. DMF, were added successively at 0 °C 0.017 g of NaH (60% in mineral oil, 1.2 eq.), 0.054 g (1 eq.) of Nal and 0.100 g (1 eq.) of 3-chloromethyl-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine (see below 1K]). The reaction was allowed to proceed for 5 Min. at 0 °C and for 0.5 h at ambient temperature. Pouring onto crashed ice/KHSO,, twofold extraction with AcOEt, washing with water and brine, drying over magnesium sulfate, and evaporation of the solvents, followed by flash chromatography (SiOa, hexane/AcOEt=75/25), yielded 0.126 8 of the title compound as light yellow oil.
MS:531.5 (M+H)". 2-(3-Methoxy-propyl)-4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)- idin-3- ethyl] -aminol-phenoxy)-acetic acid 0.125 g (0.24 mmol) of the above prepared (2-(3-methoxy-propyl)-4-{methyl-[2-methyl- 6- (4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]-amino}-phenoxy)-acetic acid ethyl ester was dissolved in 1.44 ml of THF/EtOH=1/ 1, treated with 0.72 ml (3 eq.) of IN
NaOH, and kept at ambient temperature for 0.5 bh. The reaction mixture was then poured onto crashed ice/AcOEY/HCI dil, the organic layer washed with water, dried over sodium sulfate, and evaporated to dryness. Crystallization from hexane/AcOEt produced finally 0.102 g of the title compound as off-white crystals of mp. 72-73 °C.
MS:501.1 (M-H).
The reagent used in 11] was prepared as follows:
K]_3-Dimethylamino-1-(4-trifluoromethyl-phenyl)-propan-1-one hydrochloride 4-(Trifluoromethyl) acetophenone (4.97 g, 26.4 mmol), paraformaldehyde (1.586 g, 2 eq.) and dimethylamine hydrochloride (3.231 g, 1.5 eq.) were mixed together in 7 ml of
EtOH, treated with 0.08 ml of 37% HC), and heated to reflux for 5h. Cooling down to ambient temperature, filtration and washing with tiny amounts of cold EtOH delivered 4.59 g of the title compound as white crystals, mp. 128-42 °C (dec.).
MS: 246.3 (M+H)".
L] 2-Methyl-6-(4-trifluoromethyl-phenyl)-nicotinic acid methyl ester 4.59 g (16.3 mmol) of the above prepared 3-dimethylamino-1-(4-trifluoromethyl- phenyl)-propan-1-one hydrochloride and 1.86 g (1.0 eq.) of 3-aminocrotonic acid ~~ methyl ester was dissolved in 50 ml of AcOH and heated to reflux for 4h. After cooling, the bulk of the solvent was evaporated i. V., the residue dissolved in AcOEt, and washed with water and brine. Drying over sodium sulfate, evaporation of the solvents and flash chromatography (SiO,, hexane/AcOEt=8/2) delivered finally 2.40 g of the title compound as light yellow waxy solid.
MS: 296.1 (M+H)".
M] [2-Methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-methanol 1.00 g (3.39 mmol) of the above synthesized 2-methyl-6-(4-trifluoromethyl-phenyl)- nicotinic acid methyl ester in 7 ml of abs. THF was cooled down to 0 °C and reacted with 7.06 ml of DIBAL-H-solution (1.2 M in toluene, 2.5 eq.) for 1 h. Careful quenching with ice / NH4C], twofold extraction with AcOEt, washing with brine, drying over sodium sulfate, and evaporation of the solvents left a crude product which was purified by flash chromatography (SiO, hexane/AcOEt=7/3) to deliver finally 0.875 g of the title compound as off-white solid, mp. 76-78 °C.
MS: 268.1 (M+H)".
N] 3-Chloromethyl-2-methyl-6-(4-trifluoromethyl-phenyl)-pyri dine 0.875 g (3.27 mmol) of the above prepared [2-methyl-6-(4-trifluoromethyl-phenyl)- pyridin-3-yl]-methanol was dissolved in 16 ml of CH,Cl; and treated dropwise at 0°C with 0.48 ml (2 eq.) of SOCl,. The reaction mixture was kept at 0 °C for 5 Min. and at RT for 30 Min. Pouring onto crashed ice / NaHCO, twofold extraction with AcOEt, washing with water, drying over sodium sulfate and evaporation of the solvents afforded 0.875 g "of pure title compound as light yellow oil.
MS: 285.1, 287.1 (M)™.
Example 2 (2-(3-Methoxy-propyl)-4-{methyl-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3- yimethyl]-amino}-phenoxy)-acetic acid
A] 3-Chloromethyl-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine was prepared in analogy to example 1N], but starting the whole reaction sequence with 3-(trifluoromethyl) acetophenone instead of 4- (trifluoromethyl) acetophenone, as white crystals of mp. 73-75 °C.
Co ._ MS:285.1,287.1 (M)*.
B] (2-(3-Methoxy-propyl)-4-{methyl-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin- 3-ylmethyl] -amino}-phenoxy)-acetic acid
The title compound was prepared in analogy to example 1, but using in step I] 3- chloromethyl-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine instead of 3-
chloromethyl-2-methyl-6-(4-triftuoromethyl-phenyl)-pyridine, as light brown viscous oil.
MS: 501.1 (M-H)".
Example 3 [rac)-[2-(3-Methoxy-propyl)-4-(methyl-{1-[2-methy}-6-(3-trifluoromethyl-phenyl)- pyridin-3-yl]-ethyl}-amin 0)-phenoxy]-acetic acid
Al] 2-Methyl-6-(3-trifluoromethyl-phen 1)-pyridine-3-carbaldehyde 3.00 g (11.2 mmol) of [2-methyl-6-( 3-trifluoromethyl-phenyl)-pyridin-3-yl]-methanol (synthesized as described in example 1K]-M], but starting with 3- (trifluoromethyl) acetophenone instead of 4-(trifluoromethyl) acetophenone), was dissolved in 56 ml of
CH,CJ, and treated with 14.6 g (15 eq.) of MnO,. After vigorous stirring for 2 hat ambient temperature, the reaction mixture was filtered over Celite and carefully rinsed with CH,Cl,. Evaporation of the solvent left 2.659 g of the title compound as white crystals of mp. 61-63 °C.
MS: 266.2 (M+H)".
B] [racl- 1-[2-Methyl-6-(3-trifluoromethyi-phenyl )-pyridin-3-yl]l-ethanol 0.500 g (1.89 mmol) of the above prepared 2-methyl-6-(3-trifluoromethyl-phenyl)- pyridine-3-carbaldehyde was dissolved in 9.4 ml of abs. THF and treated at -10 °C with 0.94 ml of 3M methyl magnesium chloride solution (in THF). After 30 Min., the reaction mixture was carefully poured onto crashed ice / NH,CI, extracted twice with AcOEt, washed with water, dried over sodium sulfate, and evaporated to dryness to leave 0.532 g of the title product, pure according to NMR.
MS: 282.1 (M+H)".
C] [rac]- 3-(1-Chloro-ethyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine 0.530 g (1.89 mmol) of the above prepared [rac]- 1-[2-methyl-6-(3-trifluoromethyl- . phenyl)-pyridin-3-yl]-ethanol was dissolved in 9.5 ml of CH;Cl, and treated dropwise at 0°C with 0.275 ml (2 eq.) of SOCL,. The reaction mixture was kept at 0 °C for 5 Min. and at RT for 30 Min. Pouring onto crashed ice / NaHCOs, twofold extraction with EtOEt, washing with water, drying over sodium sulfate and evaporation of the solvents yielded 0.586 g of pure title compound as light yellow oil.
D] [rac]-[2-(3-Methoxy-propyl)-4-(methyl-{1-[2-methyl-6-( 3-triffuoromethyl-phenyl)- pyridin-3-yl-ethyl}-amino)-phenoxy]-acetic acid ethyl ester
To 0.082 g (0.29 mmol) of the above (example 1H]) prepared [2-(3-methoxy-propyl)-4- methylamino-phenoxy]-acetic acid ethyl ester, dissolved in 1.0 m! of abs. DMF, were added successively at 0°C 0.014 g of NaH (60% in mineral oil, 1.2 eq.), 0.044 g (1 eq.) of
Nal and 0.087 g (1 eq.) of the above prepared [rac] -3-(1-chloro-ethyl)-2-methyl-6-(3- trifluoromethyl-phenyl)-pyridine. The reaction was allowed to proceed for 5 Min. at 0 °C and for 0.5 h at ambient temperature. Pouring onto crashed ice/KHSOy, twofold extraction with AcOEt, washing with water and brine, drying over magnesium sulfate, and evaporation of the solvents, followed by flash chromatography (SiOz, hexane/AcOEt=8/2), yielded 0.015 g of the title compound as light yellow oil.
MS: 545.5 (M+H)".
E] [rac]-[2-(3-Methoxy-propyl)-4-(methyl-{1-[2-methy}-6-( 3-trifluoromethyl-phenyl)- pyridin-3-yi}-ethyl}-amino)-phenoxy}-acetic acid 0.015 g (0.03 mmol) of the above prepared [rac]-[2-(3-methoxy-propyl)-4-(methyl-{1- [2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl] -ethyl} -amino)-phenoxy]-acetic acid ethyl ester was dissolved in 0.2 ml of THF/EtOH=1/1, treated with 0.09 ml (3 eq.) of 1N NaOH, and kept at ambient temperature for 0.5 h. The reaction mixture was then poured onto crashed ice/AcOEY/HCI dil., the organic layer washed with water and brine, dried over sodium sulfate, and evaporated to dryness to leave 0.012 g of the title compound as light brown oil.
MS: 515.3 (M-H)".
Example 4 [rac]-[2-(3-Methoxy-propyl)-4-(methyl-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)- pyridin-3-yl]-butyl}-amino)-phenoxy]-acetic acid
A] [rac]-1-[2-Methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl] -butan-1-ol __0.600¢g (2.26 mmol) of the above prepared 2-methyl-6-(3-trifluoromethyl-phenyl)- pyridine-3-carbaldehyde (example 3 A]) was dissolved in 11.3 ml of abs. THF and treated at -78 °C with 1.2 ml of 2M propyl magnesium chloride solution (in EtOEt, 1.06 eq.).
After stirring for 15 Min. at 0 °C, the reaction mixture was carefully poured onto crashed ice / NHC, extracted twice with AcOEt, washed with water, dried over sodium sulfate, and evaporated to dryness. Flash chromatography (SiO;, hexane/AcOEt=8/2) yielded then 0.281 g of the title compound as white crystals of mp. 83-85 °C.
MS: 309.2 (M)".
B] [rac]-3-(1-Chloro-butyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine 0.276 g (0.892 mmol) of the above prepared [rac]-1-[2-methyl-6-(3-trifluoromethyl- phenyl)-pyridin-3-yl]-butan-1-ol was dissolved in 4.3 ml of CH,Cl; and treated dropwise at 0 °C with 0.13 ml (2 eq.) of SOCl,. The reaction mixture was kept at 0 °C for 5 Min. and at RT for 30 Min. Pouring onto crashed ice / NaHCOs, twofold extraction with
AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents yielded 0.289 g of pure title compound as light yellow oil.
MS: 327.2, 329.1 (M)".
C] [racl-[2-(3-Methoxy-propyl)-4-(methyl-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)- pyridin-3-v1]-butyll-amino)-phenoxy]-acetic acid ethyl ester
To 0.0944 g (0.336 mmol) of the above prepared (example 1H]) [2-(3-methoxy-propyl)- 4-methylamino-phenoxy]-acetic acid ethyl ester and 0.100 g (0.305 mmol) of the above prepared [rac]-3-(1-chloro-butyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine, dissolved in 1.9 ml of abs. DMSO, were added successively 0.0505 g of K,COs (0.366 mmol) and 0.0503 g (0.336 mmol) of Nal. The reaction was allowed to proceed for 5 h at 50 °C . Pouring onto crashed ice/NH,Cl, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO,, hexane/AcOEt=8/2), afforded 0.082 g of the title compound as light yellow oil.
MS: 573.5 (M+H)".
D1] [rac]-[2-(3-Methoxy-propyl)-4-(methyl-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)- pyridin-3-yi]-butyl}-amino)-phenoxyl-acetic acid 0.082 g (0.143 mmol) of the above prepared [rac]-[2-(3-methoxy-propyl)-4-(methyl-{1- [2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]-butyl}-amino)-phenoxy] -acetic acid ethyl ester was dissolved in 1.64 ml of THF/EtOH=1/1, treated at 0 °C with 0.43 ml (3 eq.) of IN NaOH, and kept at ambient temperature for 2 h. The reaction mixture was then neutralized with HCl dil. to pH 7, extracted with AcOEF}, the organic layer was washed with water, dried over sodium sulfate, and evaporated to dryness to leave 0.070 g of the title compound as light brown oil.
MS: 543.3 (M-H)".
Example 5 [rac]-[2-Methyl-4-(methyl-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]- butyl}-amino)-phenoxy]-acetic acid
A] The title compound was prepared in analogy to example 4, but using in step CJ (2- methyl-4-methylamino-phenoxy)-acetic acid methyl ester instead of [2-(3-methoxy- propyl)-4-methylamino-phenoxy]-acetic acid ethyl ester, as yellow foam.
MS: 587.5 (M+H)".
The necessary building block was synthesized as follows:
Bl (2-Methyl-4-nitro-phenoxy)-acetic acid methyl ester
A solution of 15.76 ml (171 mmol) of methyl bromoacetate and 25.0 g (163 mmol) of 2- methyl-4-nitrophenol in 300 ml dry acetonitrile was treated with 61.3 g (188 mmol)
Cs,CO5 and stirred at RT for 4h. The mixture was filtered, washed with acetonitrile and evaporated. The residue was suspended in 700 ml CH,Cl,, filtered and evaporated to give 36.4 g of (2-methyl-4-nitro-phenoxy)-acetic acid methyl ester as yellow crystals.
MS: 225 (M").
Cl] (4-Amino-2-methyl-phenoxy)-acetic acid methyl ester
A solution of 36.3 g (161 mmol) of the above prepared (2-methyl-4-nitro-phenoxy)- acetic acid methyl ester in 400 ml MeOH and 11.2 ml (177 mmol) AcOH was hydrogenated in the presence of 3.63 g 10% Pd/C for 7h at atmospheric pressure. After removal of the catalyst the reaction was evaporated, dissolved in toluene and evaporated(5x) to give 32.5g of (4-amino-2-methyl-phenoxy)-acetic acid methyl ester as a brown crystalline residue.
MS: 196 (M+H").
D] (4-tert-Butoxycarbonylamino-2-methyl-phenoxy)-acetic acid methyl ester
A solution of 21.1 g (108 mmol) of (4-amino-2-methyl-phenoxy)-acetic acid methyl ester "7 "in 210 ml THF was treated with of 24.7 g (111 mmol) di-tert-butyl dicarbonate and heated for 3 h at 80 °C. The solution was evaporated and purified by flash chromatography with a gradient of n-heptane: AcOEt (9:1 to 4:1) to yield 28.1 g of (4- tert-butoxycarbonylamino-2-methyl-phenoxy)-acetic acid methyl ester as a light pink crystalline residue.
MS: 295 (M1).
F] [4-(tert-Butoxycarbonyl-methyl-amino)-2-methyl-phenoxy]-acetic acid methyl ester
To an ice-cooled and stirred solution of 22.8 g (77 mmol) of the above prepared (4-tert- butoxycarbonylamino-2-methyl-phenoxy) -acetic acid methyl ester in 230 ml DMF was added within 10 min 3.7 g (55% in oil, 85 mmol) of NaH and, after 1h, 14.5 ml (232 mmol) of CH;l. The reaction was stirred at 0 °C for 2h, neutralized with aqueous 10%
KHSO,, and extracted with aqueous 10% KHSO4/Et;O (3x). The organic phases were washed with aqueous 10% NaCl, dried over sodium sulfate, and evaporated to give 25.8 8 of [4-(tert-butoxycarbonyl-methyl-amin 0)-2-methyl-phenoxy]-acetic acid methyl ester.
MS: 310 (M+H").
Fl (2-Methyl-4-methylamin o-phenoxy)-acetic acid methyl ester
A solution of crude 25.8 g (77 mmol) of the above prepared [4-(tert-butoxycarbonyl- methyl-amino)-2-methyl-phenoxy}-acetic acid methyl ester in 600 ml CH,Cl; was treated at 0 °C with 198 ml TFA and stirred at RT for 30 min. The reaction was evaporated and treated with chilled aqueous saturated NaHCOj solution/Et;O (3x). The organic phases were washed with aqueous 10% NaCl, dried over sodium sulfate, and evaporated to give 16.2 g of crude product. Purification by flash chromatography on SiO, with a gradient of n-heptane: AcOEt (9:1 to 4:1) yielded 12.7g of (2-methyl-4- methylamino-phenoxy)-acetic acid methyl ester as an orange oil.
MS: 210 M+HT).
Example 6 [rac]-(4-{Methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl}-amino}- 2-trifluoromethyl-phenoxy)-acetic acid
A) 4-Nitro-2-trifluoromethyl-phenol
A solution of 24.73 g (110.7 mmol) 6-methoxy-5-nitrobenzotrifluoride in 260 ml acetic acid and 130 ml of aqueous HBr solution (62%) was heated to reflux for 96 h, cooled ~~ down, evaporated and taken up imraqueous saturated NaHCO3 solution/Et,O (3x). The organic phases were washed with aqueous 10% NaCl, dried over sodium sulfate, and evaporated to dryness to yield 19.27 g of the title compound as yellow solid of mp. 103- 104°C.
MS: 207 (M™).
B] (4-Methylamino-2-trifluoromethyl-phenoxy)-acetic acid ethyl ester
Analogously to example 5B] to 5F), 4-nitro-2-trifluoromethyl-phenol and ethyl bromoacetate were transformed to (4-methylamino-2-trifluoromethyl-phenoxy)-acetic acid ethyl ester containing some (4-methylamino-2-trifluoromethyl-phenoxy)-acetic acid methyl ester as light yellow waxy solid.
MS: 278 (M+H") and 264 (M+H").
C] [racl- (4-{Methyl-[2-methy]-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]- amino}-2-trifluoromethyl- phenoxy)-acetic acid ethyl ester
To 0.080 g (0.29 mmol) of the above prepared (4-methylamino-2-trifluoromethyl- phenoxy)-acetic acid ethyl ester and 0.124 g (0.43 mmol) of 3-chloromethyl-2-methyl-6- (4-trifluoromethyl-phenyl)-pyridine (see example IN], dissolved in 1.0 ml of abs.
DMSO, were added successively 0.048 g (0.32 mmol) of Nal and 0.066 g (0.43 mmol) of
DBU. The reaction was allowed to proceed for 4 h at ambient temperature, when TLC still indicated some starting amine. Therefore, another 33 mg (0.4 eq.) of 3- chloromethyl-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine was added and stirring continued for 3 h. Pouring onto crashed ice, extraction with AcOEt, filtration over Celite, washing of the organic layer with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO, hexane/AcOEt=8/2), afforded 0.046 g of the title compound as colorless oil.
MS: 527.5 (M+H)".
D] [racl-(4-{Methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]- amino}-2-trifluoromethyl-phenoxy)-acetic acid 0.046 g (0.087 mmol) of the above prepared [rac]- (4-{methyi-[2-methyl-6-(4- triffuoromethyl-phenyl)-pyridin-3-ylmethyl]-amino}-2-trifluoromethyl-phenoxy)-acetic acid ethyl ester was dissolved in 0.52 ml of THF/EtOH=1/1, treated with 0.26 ml (3 eq.) of 1N NaOH and kept at ambient temperature for 3.5 h. The reaction mixture was then poured onto crashed ice/AcOEt/HCI dil., the organic layer was washed with water, dried over sodium sulfate, and evaporated to dryness to leave, after recrystallisation from =" - AcOEt/hexane, 0.039 g of the title compound as off-white solid.
MS: 497.0 (M-H).
Example 7 (2,6-Dimethyl-4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]- amino}-phenoxy)-acetic acid
A] The title compound was prepared in analogy to example 6, but using in step CJ (2,6- dimethyl-4-methylamino-phenoxy)-acetic acid ethyl ester (described in US Patent No. 5905068) instead of (4-methylamino-2-trifluoromethyl-phenoxy)-acetic acid ethyl ester, as brownish crystals of mp. 137-138 °C.
MS: 459.6 (M+H)".
Example 8 2-Methyl-2-{2-methyl-4-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]- phenoxy}-propionic acid
A] 2-Methyl-2-{2-methyl-4-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3- ylmethoxy]-phenoxyl-propionic acid ethyl ester
To 0.150 g (0.629 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) and 0.180 g (0.629 mmol) of the above prepared (example 2A]) 3-chloromethyl-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine, dissolved in 5.4 ml of abs. acetonitrile, was added 0.226 g of Cs;CO; (0.692 mmol). The reaction was stirred for 3.5 h at ambient temperature. Pouring onto crashed ice, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO;, hexane/AcOEt=85/15) yielded 0.248 g of pure title compound as colorless oil.
MS: 488.4 (M+H)".
B] 2-Methyl-2-{2-methyl-4-[2-methyl-6-(3-triftuoromethyi-phenyl)-pyridin-3- ylmethoxy)]-phenoxyl-propionic acid 0.248 g (0.509 mmol) of the above prepared 2-methyl-2-{2-methyl-4-[2-methyl-6-(3- trifluoromethyl-phenyl)-pyridin-3-ylmethoxy] -phenoxy}-propionic acid ethyl ester was - -- + dissolved in 5.0 ml of THF/EtOH=1/1; treated with 1.50 ml (3 eq.) of IN NaOH and kept = at ambient temperature for 20 h. The reaction mixture was then poured onto crashed ice/ AcOEt/HCI dil. to bring the pH to 7, the organic layer was washed with water, dried over sodium sulfate, and evaporated to dryness to leave, after recrystallisation from
AcOEt/hexane, 0.224 g of the title compound as white crystals of mp. 105-107 °C.
MS: 458.2 (M-H)".
Example 9 2-Methyl-2-{2-methyl-4-[6- (4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy}- phenoxy}-propionic acid
A] The title compound was prepared in analogy to example 8, but using in step A] 5- chloromethyl-2-(4-trifluoromethyl-phenyl)-pyridine instead of 3-chloromethyl-2- methyl-6-(3-trifluoromethyl-phenyl)-pyridine, as white solid of mp. 149-151 °C.
MS: 444.1 (M-H)".
B] 5-Chloromethyl-2-(4-trifluoromethyl-phenyl)-pyridine
This reagent, necessary in step A], was prepared in analogy to example 1N] from [6-(4- trifluoromethyl-phenyl)-pyridin-3-yl]-methanol (described in WO 01/060805) as white solid of mp. 73 °C.
MS: 272.1, 274.1 (M+H)".
Example 10 2-Methyl-2-{2-methyl-4-[6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy]- phenoxy}-propionic acid
A] The title compound was prepared in analogy to example 8, but using in step A] 5- chloromethyl-2-(4-trifluoromethoxy-phenyl)-pyridine instead of 3-chloromethyl-2- methyl-6-(3-trifluoromethyl-phenyl)-pyridine, as white solid of mp. 146-148 °C.
MS: 460.2 (M-H)".
The reagent, necessary in step A], was prepared as follows:
B| [6-(4-Trifluoromethoxy-phenyl)-pyridin-3-yll-methanol 2.00 g (7.48 mmol) of commercially available 6-(4-trifluoromethoxy-phenyl) -pyridine-3- _. carbaldehyde was dissolved in 37 ml of EtOH and treated at 0 °C with 0.283 g (7.48 . mmol) of NaBH,. After 10 Min. the cooling bath was removed and stirring continued at ambient temperature. Pouring onto crashed ice, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents afforded 2.08 g of pure title compound as off-white solid of mp. 57-58 °C.
MS: 269.1 (M)".
C] 5-Chloromethyl-2-(4-trifluoromethoxy-phenyl)-pyridine 0.400 g (1.49 mmol) of the above prepared [6-(4-trifluoromethoxy-phenyl)-pyridin-3- y1]-methanol was dissolved in 7.2 ml of CHCl and treated dropwise at 0 °C with 0.22 ml (2 eq.) of SOCl,. The reaction mixture was kept at 0 °C for 5 Min. and at RT for 30 Min.
Pouring onto crashed ice / NaHCO3, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents produced 0.419 g of pure title compound as off-white solid of mp. 34-36 °C.
MS: 288.1, 290.1 (M+H)".
Example 11 [rac]-[2-Methyl-4-(methyl-{1-[2-methy}-6-(3-triffuoromethyl-phenyl)-pyridin-3-yl}- propyl}-amino)-phenoxy]-acetic acid
A) The title compound was prepared in analogy to example 5, but using in step C] [rac]- 3-(1-chloro-propyl)-2-methyl-6-(3-trifiuoromethyl-phenyl)-pyridine instead of [rac]-3- (1-chloro-butyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine, as yellow solid.
MS: 471.1 (M-H).
The necessary intermediate
B] [rac]-3-(1-Chloro-propyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine
Was prepared as described in example 4A]-B], but using ethyl magnesium chloride instead of propyl magnesium chloride, as yellowish oil.
MS: 314.11, 316.2 (M+H)".
Example 12 (4-{[6-(4-Chloro-phenyl)-pyridin-3-ylmethyl] -methyl-amino}-2-methyl-phenoxy)- acetic acid
A] The title compound was prepared in analogy to example 4, but using in step C] (2- : 75 methyl-4-methyliriitio-phenoxy)-aceticacid ethyl ester (prepared in analogy to example 5F))instead of [2-(3-methoxy-propyl)-phenoxy]-acetic acid ethyl ester and 5- chloromethyl-2-(4-chloro-phenyl)-pyridine instead of [rac]-3-(1-chloro-butyl)-2- methyl-6-(3-trifluvoromethyl-phenyl)-pyridine, as off-white crystals of mp. 174-76 °C.
MS: 397.2, 399.4 (M+H)".
The necessary intermediate
B] 5-Chloromethyl-2-(4-chloro-phenyl)-pyridine ‘Was prepared in analogy to example 1N] from [6-(4-chloro-phenyl)-pyridin-3-yl}- methanol as light brown solid.
MS: 237.1,239.1,241.1 (M)".
Example 13 (2-Methyl-4-{methyl- [6-(4-trifluoromethoxy-phenyl)-pyridin-3-yimethyl] -amino}- phenoxy)-acetic acid
A) The title compound was prepared in analogy to example 12, but using in step C] 5- chloromethyl-2-(4-trifluoromethoxy-phenyl)-pyridine (example 10C})instead of 5- chloromethyl-2-(4-chloro-phenyl)-pyridin, as off-white solid of mp. 137-138 °C.
MS: 445.2 (M-H)".
Example 14 (2- (3-Methoxy-propyl)-4-{methy}-[6-(4-triffuoromethoxy-phenyl)-pyridin-3-yimethyl]- amino}-phenoxy)-acetic acid
A) The title compound was prepared in analogy to example 13, but using in step Cl] (2- (3-methoxy-propyl)-4-methylamino-phenoxy]-acetic acid ethyl ester (example 1H}) instead of (2-methyl-4-methylamino-phenoxy)-acetic acid ethyl ester, as light brown solid of mp. 73-75 °C.
MS: 505.5 (M+H)".
Example 15 (2-Methyl-4- {methyl-[6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]-amino}- phenoxy)-acetic acid oo oo . .... A] The title compound was prepared in analogy to. example 13, but using in step C] 5- chloromethyl-2-(4-trifluoromethyl-phenyl)-pyridine instead of 5-chloromethyl-2-(4- triftuoromethoxy-phenyl)-pyridine, as off-white solid of mp. 199 °C.
MS: 431.3 (M+H)*.
The necessary intermediate
B] 5-Chloromethyl-2-(4-triflucromethyl-phenyl)-pyridine was prepared in analogy to example 10C] as white solid of mp. 73 °C.
MS: 272.1, 274.1 (M+H)".
Example 16 (2-(3-Methoxy-propyl)-4-{methyl-[6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]- amino}-phenoxy)-acetic acid
The title compound was prepared in analogy to example 15, but using in step C] [2-(3- methoxy-propyl)-4-methylamino-phenoxy]-acetic acid ethyl ester instead of (2-methyl- 4-methylamino-phenoxy)-acetic acid methyl ester, as light brown solid of mp. 72-76 °C.
MS: 487.2 (M-H)".
Example 17 [rac]-(2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl}- ethylsulfanyl}-phenoxy)-acetic acid
Al (2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] -ethylsulfanyl}- phenoxy)-acetic acid tert-butyl ester 0.224 g (0.75 mmol) of [rac]-3-(1-chloro-ethyl)-2-methyl-6-(4-trifluoromethyl-phenyl)- pyridine and 0.222 g (0.75 mmol) of (4-acetylsulfanyl-2-methyi-phenoxy)-acetic acid tert-butyl ester (prepared by standard acetylation of (4-mercapto-2-methyl-phenoxy)- acetic acid tert-butyl ester, described below in example 28A)-C}) were dissolved in 3.9 ml of acetonitrile and 0.2 ml of MeOH and treated with 0.292 g (1.2 eq.) of Cs,COs. The reaction was then allowed to proceed over night. Pouring onto crashed ice, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO,, hexane/AcOFEt=9/1), afforded in the less polar fractions 0.210 g of pure title compound and in the more polar fractions 0.112 g of the corresponding methyl ester as waxy solid. CL
BI rac]-(2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)- idin-3-yl]- ethylsulfanyl}-phenoxy)-acetic acid 0.322 g (0.62 mmol) of the above prepared (2-methyl-4-{1-[2-methyl-6-(4- trifluoromethyl-phenyl)-pyridin-3-yi]-ethylsulfanyl} -phenoxy)-acetic acid tert-butyl ester, containing some methyl ester, was dissolved in 3.8 ml of THE/EtOH=1/1, treated with 1.87 ml (3 eq.) of IN NaOH, and kept at ambient temperature for 2 h. The reaction mixture was then poured onto crashed ice/HCI dil, extracted twice with AcOEt, washed with water, dried over sodium sulfate, and evaporated to dryness to leave, after crystallization from AcOEt / hexane, 0.276 g of the title compound as off-white solid of mp. 115-117°C.
MS: 460.3 (M-H)".
The necessary reagent
Cllrac]-3-(1-Chloro-ethyl)-2-methyl-6-(4-trifluoromethyl-phenyl )-pyridine
Was prepared in analogy to example 3A]-C], but starting the whole reaction sequence with 4-(trifluoromethyl) acetophenone instead of 3-(trifluoromethyl) acetophenone), as colorless oil.
MS: 299.1, 301.1 (M)".
Example 18 [rac]-(2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- propylsulfanyl}-phenoxy)-acetic acid
A] The title compound was prepared in analogy to example 17, but using [rac]-3-(1- chloro-propyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine instead of [rac]-3-(1- chloro-ethyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine, as off-white solid of mp. 126-128 °C.
MS: 474.1 (M-H)". . .. ...Thenecessary reagent
Bl[rac]- 3-(1-Chloro-propyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine was prepared in analogy to example 17C], but using for the Grignard-reaction ethyl magnesium chloride instead of the methyl derivative, as colorless oil.
MS: 314.2, 316.2 (M+H)".
Example 19 [rac]-(2-Methyl-4-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl}- ethylsulfanyl}-phenoxy)-acetic acid
A] The title compound was prepared in analogy to example 17, but using [rac]-3-(1- chloro-ethyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine (example 3C]) instead of [rac]-3-(1-chloro-ethyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine, as colorless foam.
MS: 460.3 (M-H)".
Example 20 [rac]-(2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-ethoxy}- phenylsuifanyl)-acetic acid
Al [rac[- (2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi]-ethoxy}- phenylsulfanyl)-acetic acid tert-butyl ester
To 0.118 g (0.39 mmol) of the above prepared {rac} -3-(1-chloro-ethyl)-2-methyl-6-(4- trifluoromethyl-phenyl)-pyridine (example 17C]) and 0.100 g (0.39 mmol) of (4- hydroxy-2-methyl-phenylsulfanyl)-acetic acid tert-butyl ester (see below example 20C]), dissolved in 2.0 ml of abs. acetone, were added successively 0.065 g (0.39 mmol) of KI and 0.154 g (0.47 mmol) of Cs,CO;. The reaction was allowed to proceed for 2 h at ambient temperature, when TLC still indicated large amounts of starting material.
Therefore, stirring was continued over night at 40 °C. Pouring onto crashed ice, extraction with AcOEt, filtration over Celite, washing of the organic layer with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO;, hexane/AcOEt=9/1), produced 0.143 g of the title compound as colorless oil.
MS: 518.4 (M+H)". “- ° B] [rac]-(2-Methyl-4-{ 1-[2-meth§l-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] -ethoxy}- phenylsulfanyl)-acetic acid 0.143 g (0.28 mmol) of the above prepared [rac]-(2-methyl-4-{1-[2-methyl-6-(4- trifluoromethyl-phenyl)-pyridin-3-yl]-ethoxy}-phenylsulfanyl)-acetic acid tert-butyl ester was dissolved in 2.75 ml of THF/EtOH=1/1, treated with 1.38 ml (3 eq.) of IN
NaOH and kept at ambient temperature for 5 h. The reaction mixture was then poured onto crashed ice/AcOEt/HCI dil., the organic layer was washed with water, dried over sodium sulfate, and evaporated to dryness to leave, since recrystallisation from
AcOEt/hexane failed, 0.095 g of the title compound as colorless foam.
MS: 460.1 (M-H)".
The necessary reagent used in 20A] was prepared as follows:
C] (4-Hydroxy-2-methyl-phenylsulfanyl )-acetic acid tert-butyl ester
A solution of 10 g (71 mmol) of 4-mercapto-3-methyl-phenol (DE 2101359) and 10.5 ml (1 eq., 71 mmol) of bromo-acetic acid tert-butyl ester in 250 ml of THF was treated at room temperature with 12.2 mi (1 eq., 71 mmol) of N-ethyldiisopropylamine. The suspension was heated at reflux for 1 h, cooled to ambient temperature, and treated with water. The reaction mixture was then poured onto 0.1 M HCI/AcOEL, the organic layer was separated, washed with water and brine, dried over magnesium sulfate, and evaporated to dryness. Purification by flash chromatography (SiO, heptane/toluene/
AcOEt = 10/1/1) afforded finally 5.6 g of the title product as white solid.
MS: 253 (M-H)".
Example 21 [rac]-(2-Methyl-4-{1-[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl]-butoxy}- phenylsulfanyl)-acetic acid
A] The title compound was prepared in analogy to example 20, but using [rac]- 3-(1- chloro-butyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine instead of [rac]-3-(1- chloro-ethyl)-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine, as colorless foam.
MS: 488.1 (M-H)".
The necessary reagent B] [racl- 3-(1-chloro-butyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine oo Was prepared in analogy to exam ple 17C), but using for the Grignard-reaction propyl magnesium chloride instead of the methyl derivative, as colorless oil.
MS: 328.2, 330.3 (M+H)".
Example 22 [rac]-(2-Methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl}- butylsulfanyl}-phenoxy)-acetic acid
A] The title compound was prepared in analogy to example 17, but using in step A) [rac]- 3-(1-chloro-butyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine (example 21B)) instead of [rac] -3-(1-chloro-ethyl)-2-methyl-6-(4-trifluoromethyl-phenyl)- pyridine, as colorless foam.
MS: 488.0 (M-H)".
Example 23 [rac]-(4-{Cyclopentyl-{2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-y1]- methylsulfanyl}-2-methyl-phenoxy)-acetic acid
A) The title compound was prepared in analogy to example 17, but using in step A] [rac]- 3-(chloro-cyclopentyl-methyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine instead of [rac]-3-(1-chloro-ethyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine, as colorless foam.
MS: 514.2 (M-H)".
Example 24 2-{4-[6-(4-Fluoro-3-trifluoromethyl-phenyl)-2-methyl-pyridin-3-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid
A) The title compound was prepared in analogy to example 8, but using in step A] 3- chloromethyl-6-(4-fluoro-3-trifluoromethyl-phenyl)-2-methyl-pyridine instead of 3- chloromethyl-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine, as white crystals of mp. 141-143 °C. .
MS: 476.1 (M-H)".
Thenecessaryreagent
Bl 3-Chloromethyl-6-(4-fluoro-3-trifluoromethyl-phenyl)-2-methyl-pyridine was prepared in analogy to example 1K]-N], but starting the whole reaction sequence with 1-(4-fluoro-3-trifluoromethyl-phenyl)-ethanone instead of 4- (trifluoromethyl) acetophenone, as off-white solid.
MS: 303.1, 305.1 (M)".
Example 25 [rac] -2-Methyl-2-(2-methyl-4-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]- ethoxy}-phenoxy)-propionic acid
A] The title compound was prepared in analogy to example 8, but using in step A] (racl- 3-(1-chloro-ethyl) -2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine (example CD instead of 3-chloromethyl-2-methyl-6-(3-trifluoromethyl-phenyl)-pyridine, as white solid.
MS: 472.1 (M-H)".
Example 26 2-Methyl-2-[3-(methyl-{2-[2-methy}-6- (4-trifluoromethyl-phenyl)-pyridin-3-yl}- acetyl}-amino)-phenoxy]-propionic acid
A] 2-Methyl-2-(3-methylamino-phenoxy)-propionic acid ethy] ester
In analogy to the procedures described in example 5D], 5E] and 5F}, 2-(3-amino- phenoxy)-2-methyl-propionic acid ethyl ester [PCT Int. Appl. WO 2003/063794 A2] was converted into the corresponding tert-butoxycarbonylamino derivative, methylated at N and deprotected again to yield the title compound as light yellow oil.
MS: 238.1 (M+H)*+; 224.1 [(M+H)™ of the corresponding methyl ester present as impurity]. B] 2-Methyl-2-{3-(methyl-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl|- acetyl}-amino)-phenoxyl-propionic acid ethyl ester 0.24 g (1.00 mmol) of the above prepared 2-methyl-2-(3-methylamino-phenoxy)- propionic acid ethyl ester and 0.31 g (1.05 mmol) of [2-methyl-6-(4-trifluoromethyl- phenyl)-pyridin-3-yl]-acetic acid (example 26E]) were dissolved in 15 ml of CHzCl,. This solution was cooled down to 0°C and then 0.24 g (1.20 mmol) of N-(3-dimethylamino- ... propyl)-N’-ethyl-carbodiimide-hydrochloride and 0.19 g (1.50 mmol) of N,N- dimethylaminopyridine were added and the reaction stirred for 20 hours at ambient temperature. It was subsequently poured into crashed ice / HCI and extracted two fold with CH,Cly; the organic layers were washed with water, dried over magnesium sulfate, filtered and evaporated. The crude product was purified by chromatography (SiOz; n- heptane / AcOEt = 95:5 to 4:1) to give 0.50 g of the title compound as a light yellow oil.
MS: 515.3 (M+H)".
C] 2-Methyl-2-[3-(methyl-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yli- acetyl}-amino)-phenoxyl-propionic acid 0.50 g (0.97 mmol) of the above prepared 2-methyl-2-{3-(methyl-{2-[2-methyl-6-(4- trifluoromethyl-phenyl)-pyridin-3-yl]-acetyl}-amino)-phenoxy]-propionic acid ethyl ester was dissolved in 15 ml of THE / MeOH = 2:1. To the stirred solution was added 1.16 ml of a LiOH-solution (1 molar in water). After 6 hours, the reaction mixture was poured into crashed ice / HCl and extracted twice with CH,Cl,; the organic layers were washed with water, dried over magnesium sulfate, filtered and evaporated to give 0.47 g of pure title compound as colorless foam.
MS: 485.3 (M-H).
The necessary building block [2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi}- acetic acid used in procedure 26B] was prepared as follows:
D] [2-Methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] acetonitrile 27.2 g (95.2 mmol) of 3-chloromethyl-2-methyl-6-(4-triftuoromethyl-phenyl)-pyridine (example 1N]) was dissolved in 100 ml of dimethyl sulfoxide; 5.9 g of sodium cyanide (120 mmol) was added and the mixture was stirred at room temperature for 18 hours.
Then, the reaction mixture was poured into a mixture of ice and water and was subsequently extracted with 3 portions of 400 ml of tert-butyl methyl ether. The combined organic phases were washed with water, then with brine and dried over anhydrous sodium sulfate. After evaporation of the solvent, 25.2 g of the title compound was obtained as a pale yellow solid.
MS: 276.1 (M)™.
E] [2-Methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl1-acetic acid
A mixture of 25 g (90 mmol) of the above prepared [2-methyl-6-(4-trifluoromethyl- phenyl)-pyridin-3-yl}-acetonitrile, 20 g of sodium hydroxide (500 mmol), 60 ml of water and 250 ml of propanol was stirred vigorously at 100 °C. Hydrolysis was complete after 2 hours. The reaction mixture was then evaporated to dryness and the residue was dissolved in 70 ml of water; then, 60 ml of cold 8 N aqueous HC] was added and the compound was extracted with three portions of 250 ml of ethyl acetate; the combined organic phases were washed with water and brine, dried over anhydrous sodium sulfate and evaporated to dryness to yield 25.1 g of the title product as pale yellow solid.
MS: 296.0 (M+H)".
Example 27 (4-{[4-Cyclopropyl-2-(4-triflucromethyl-phenyl)-pyrimidin-5-ylmethyl]-methyl- amino}-2-methyl-phenoxy)-acetic acid
Al (4-{[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethyl] -methyl- amino}-2-methyl-phenoxy)-acetic acid methyl] ester :
To a solution of 400 mg (1.28 mmol) 5-chloromethyl-4-cyclopropyl-2-(4- trifluoromethyl-phenyl)-pyrimidine (example 27F]) and 268 mg (1.28 mmol) of (2- methyl-4-methylamino-phenoxy)-acetic acid methyl ester (example 5F}) in 8 ml of
DMSO was added 194 mg (1.41 mmol) potasium carbonate and 192mg (1.28 mmol) sodium iodide. The reaction mixture was stirred for 20 min at RT. It was then taken up in ether and washed with aqueous 10% KHSO4-solution (10%) and water. The crude product was purified by chromatography over silica gel with AcOEt/heptane 1:3, to give 403 mg of pure (4-{[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethyl} - methyl-amino}-2-methyl-phenoxy)-acetic acid methyl ester.
MS: 486.4 (M+H)".
BJ (4-{{4-Cyclopropyl-2-(4-trifinvoromethyl-phenyl)-pyrimidin-5-ylmethyl}-methyl- amino}-2-methyl-phenoxy)-acetic acid
A solution of 351 mg (0.72 mmol) (4-{[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)- pyrimidin-5-ylmethyl]-methyl-amino}-2-methyl-phenoxy)-acetic acid methyl ester and 1.45 ml IN LiOH in 4 ml of THF was stirred for 3h at RT. The reaction mixture was taken up in ether and washed with IN HCl and water. The crude product was suspended in AcOEt/beptane 1:19 to give 95.64 mg of pure (4-{[4-cyclopropyl-2-(4-trifluoromethyl- phenyl)-pyrimidin-5-ylmethyl]-methyl-amino}-2-methyl-phenoxy)-acetic acid.
MS: 472.3 (M+H)". 5-Chloromethyl-4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine used in 27A} was synthesized as follows:
Cl (E.Z)-2-Cyclopropanecarbonyl-3-ethoxy-acrylic acid methyl ester
A solution of 10 g (70.34 mmol) 3-cyclopropyl-3-oxo-propionic acid methyl ester, 23.4 ml (140.68 mmol) of triethyl orthoformate in 100 ml acetic anhydride was refluxed at
150 °C, for 5h. The reaction mixture was concentrated at 95 °C under reduced pressure to give 14.35 g of crude (E,Z)-2-cyclopropanecarbonyl-3-ethoxy-acrylic acid methyl ester.
MS: 199.3 (M+H)".
D] 4-Cydopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5-carboxylic acid ethyl ester
To a solution of 4.74 g (18.19 mmol) 4-trifluoromethyl-benzamidine HCl in 50 ml of ethanol was added 1.818 g (18.186 mmol) of sodium tert-butoxide. After 2 min, 3.605 g of crude (E,Z)-2-cyclopropanecarbonyl-3-ethoxy-acrylic acid methyl ester was added and the reaction mixture was then stirred over night at RT. The ethanol was removed under reduced pressure, the residue taken up in ether and washed with IN HCl and water. The ether solution was concentrated under reduced pressure and the crude product purified by chromatography over silica gel with AcOEt/heptane 1:3 to give 4.25 g of pure 4- cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5-carboxylic acid ethyl ester.
MS: 337.1 (M+H)"
El [4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-methanol
Within 10 min was dropped 31.6 ml (37.9 mmol) of 1.2 M DIBALH solution in toluene to a dry ice cooled (-50 °C) solution of 4.25 g (12.64 mmol) 4-cyclopropyl-2-(4- trifluoromethyl-phenyl)-pyrimidine-5-carboxylic acid ethyl ester in 50 ml of THF. The reaction mixture was stirred 30 min at -50 °C and after letting rise the temperature to RT, the reaction was stirred for 1h at RT. The reaction mixture was taken up in ether and washed with 1N HCL and water. The solvent was removed under reduced pressure to give 3.72 g of pure [4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- methanol. :
MS: 295.1 (M+H)*.
F] 5-Chloromethyl-4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine
A mixture of 1.9 g (6.456 mmol) of [4-cyclopropyl-2-(4-trifluoromethyl-phenyl)- pyrimidin-5-yl]-methanol and 0.515 ml (7.1 mmol) thionylchloride in 20 ml _ dichloromethane was stirred for 1h at RT, The reaction mixture was taken up in ether and washed with sodium bicarbonate solution and water. The ether phase was concentrated under reduced pressure to give 1.97 g of pure 5-chloromethyl-4- cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidine.
MS: 313.1 (M+H)". :
Example 28 [rac]-(4-{1-[4-Cydlopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- ethylsulfanyl}-2-methyl-phenoxy)-acetic acid
Al 4, 4 -Dithio-bis-(2-methyl-phenol
To a solution of 5 g (46.23 mmol) o-cresol and 6.15 ml (48.54 mmol) chloro-trimethyl- silane in 50 ml dichloromethane was dropped under ice cooling 6.76 ml (48.55 mmol) triethyl amine within 5 min. The reaction mixture was stirred at RT for 30 min and then it was chilled to -40 °C. At this temperature was dropped 1.86 ml (23.13 mmol) di-sulfur- di-chloride to the mixture, within 10 min. The reaction was allowed to come to RT and was stirred at this temperature for 1h. The lot was taken up in ether and washed with water. The crude product was purified by chromatography over silica gel with AcOEY/ heptane 1:3 providing 4 g pure 4, 4’-dithio-bis-(2-methyl-phenol).
MS: 277.3 (M-H).
B] [4-(4-tert-Butoxycarbonylmethoxy-3-methyl-phenyldisulfanyl)-2-methyl-phenoxy]- acetic acid tert-butyl ester
To an ice cooled solution of 4.75 g (17.06 mmol) 4, 4’-dithio-bis-(2-methyl-phenol) and 5.55 ml (37.6 mmol) bromo-acetic acid tert-butyl ester in 50 ml DMF was added 204 mg (85.3 mmol) sodium hydride (55% in oil). The reaction mixture was stirred over night at
RT and then it was taken up in ether, washed with 1N HCl and water. The crude product was purified by chromatography over silica gel with AcOEt/heptane 1:4 providing 3.4 g pure [4-(4-tert-butoxycarbonylmethoxy-3-methyl-phenyldisulfanyl)-2-methyi- phenoxy]-acetic acid tert-butyl ester.
MS: 524 (M+NH,)".
C] (4-Mercapto-2-methyl-phenoxy)-acetic acid tert-butyl ester
To a solution of 3.3 g (6.51 mmol) [4-(4-tert-butoxycarbonylmethoxy-3-methyl- phenyldisulfanyl)-2-methyl-phenoxy]-acetic acid tert-butyl ester in 30 ml - dichloromethane was added under ice cooling 4.26 g (65.1 mmol) zinc powder, 15 ml
N acetic acid and a few drops of HCI (25%). After a few minutes, the reaction completed.
Then the solution filtered, taken up in ether and washed with water. The ether solution was concentrated under reduced pressure, providing 2.86 g of almost pure (4-mercapto- 2-methyl-phenoxy)-acetic acid tert-butyl ester.
MS: 225 (M+H)*.
D1] [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl|- ethylsulfanyl}-2-methyl-phenoxy)-acetic acid tert-butyl ester
To a solution of 180 mg (0.551 mmol) [rac]-5-(1-chloro-ethyl)-4-cyclopropyl-2-(4- trifluoromethyl-phenyl)-pyrimidine (example 28H]) and 140 mg (0.551 mmol) (4- mercapto-2-methyl-phenoxy)-acetic acid tert-butyl ester in 3 ml DMF was added 315 mg (0.66 mmol) cesium carbonate. The reaction mixture was stirred for 3 h at RT and then partitioned between ether, IN HCl and water. The crude product was purified by chromatography over silica gel with AcOEt/Heptan 1:9 to give 150 mg of pure [rac]-(4- {1-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl) -pyrimidin-5-yl] -ethylsulfanyl}-2- methyl-phenoxy)-acetic acid tert-butyl ester.
MS: 544.64 (M+H)".
E] [rac]-(4-{1-[4-Cyclopropyl-2-(4-triflugromethyl-phenyl)-pyrimidin-5-yi] - ethylsulfanyll-2-methyl-phenoxy)-acetic acid
To asolution of 146 mg (0.268 mmol) [rac]-(4-{1-[4-cyclopropyl-2-(4-trifluoromethyl- phenyl)-pyrimidin-5-yl]-ethylsulfanyl}-2-methyl-phenoxy)-acetic acid tert-butyl ester in 2 ml dichloromethane was added 0.5 ml trifluoroacetic acid. The reaction mixture was stirred for 4h and then the trifluoroacetic acid was removed under reduced pressure. The crude product was suspended in AcOEt/heptane 1:19. The resulting crystalline product was filtered off to give 118mg of pure [rac}-(4-{1-[4-cyclopropyl-2-(4-trifluoromethyl- phenyl)-pyrimidin-5-y1]-ethylsulfanyl}-2-methyl-phenoxy)-acetic acid.
MS: 487.2 (M-H)". [rac]-5-(1-Chloro-ethyl)-4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine used in 28D] was synthesized as follows:
F] 4-Cyclopropyl-2-(4-trifluoromethyi-phenyl)-pyrimidine-5-carbaldehyde
To a dry ice cooled solution of 0.86 ml (12 mmol) DMSO in 10 ml dichloromethane was added 0.68 ml (7.79 mmol) oxalylchloride. After the reaction mixture was stirred for 5 min at -70 °C, 1.8 g (5.99 mmol) of [4-cyclopropyl-2-(4-trifluoromethyl-phenyl)- . ....... .pyrimidin-5-yl]-methanol, dissolved in 8. ml dichloromethane, was added by dropping within 5 min. The reaction mixture was then stirred for further ten minutes at -70 °C and after 4.17 ml (30 mmol) triethylamine was added, the reaction mixture was allowed to come to RT and it was stirred for 1h at RT. The mixture was taken up in ether and washed with 1N HCl and water. The ether phase was concentrated under reduced pressure to give 1.75 g of pure 4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine- 5-carbaldehyde. .
MS: 264.1 (M-CO)
G1 [racl-1-[4-Cyclopropyl-2-(4-trifluoromethyl- phenyl) -pyrimidin-5-yl] -ethanol
To adry ice chilled solution of 0.75 g (2.57 mmol) 4-cyclopropyl-2-(4-triflucromethyl- phenyl)-pyrimidine-5-carbaldehyde in 5 ml THF was dropped a solution of 1.11 ml (3.33 mmol) 3M methylmagnesium bromide in 2.5 ml THF. The reaction mixture was allowed to warm to RT and it was stirred for 15 min at this temperature. The reaction mixture was then taken up in ether and washed with IN HCl and water. The crude product was purified by chromatography over silica gel with AcOEY/ dichloromethane 1:6, to give 384 mg of pure [rac]-1-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- ethanol.
MS: 309.1 (M+H)".
H] [rac]-5-(1-Chloro-ethyl)-4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine
A solution of 357 mg (1.16 mmol) [rac]-1-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)- pyrimidin-5-yl]-ethanol and 0.09 ml (1.22 mmol) thionyichloride in 4 ml dichloromethane were stirred for 1h at RT. The reaction mixture was concentrated under reduced pressure to provide 390 mg of pure [rac]-5-(1-chloro-ethyl)-4-cyclopropyl-2-(4- triffuoromethyl-phenyl)-pyrimidine.
MS: 327.4 (M+H)".
Example 29 rac]-(4-{1-[4-Cyclopropyl-2- (4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-ethoxy}-2- methyl-phenoxy)-acetic acid
Al [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl] -ethoxyl-2- methyl-phenoxy)-acetic acid ethyl ester 777180 mig (0.551 mmol) of [rac] -5-(1-chloro-ethyl)-4-cyclopropyl-2-(4-trifiuoromethyl- phenyl)-pyrimidine (example 28H), 116 mg (0.551 mmol) of (4-hydroxy-2-methyl- phenoxy)-acetic acid ethyl ester (W002092590) and 31mg (0.71 mmol) sodium hydride (55% in oil) were stirred in 3 ml DMF over night. The reaction mixture was partitioned between ether and water. The crude product was purified by chromatography over silica gel with AcOEt/heptane 1:4 to give 157 mg of pure [rac]- (4-{1-[4-cyclopropyl-2-(4-
trifluoromethyl-phenyl)-pyrimidin-5-yl]-ethoxy}-2-methyl-phenoxy)-acetic acid ethyl ester. ’
MS: 501.4 (M+H)".
BI Iracl-(4-{1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl] -ethoxy}-2- . 5 methyl-phenoxy)-acetic acid 154 mg (0.307 mmol) of (rac)-(4-{1-{4-cyclopropyl-2-(4-trifluoromethyl-phenyl)- pyrimidin-5-yl]-ethoxy}-2-methyl-phenoxy)-acetic acid ethyl ester and 0.62 ml IN LiOH in 1.6 ml THE were stirred for 2h at RT. The reaction mixture was taken up in ether and washed with 1N HCl and water. The crude product was suspended in AcOEt/heptane 1:19 and the resulting crystals were filtered off to give 125 mg of pure [rac)-(4-{1-[4- cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yi} -ethoxy} -2-methyl-phenoxy)- - aceticacid.
MS: 471.1 (M-H)".
Example 30 [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifftuoromethyl-phenyl)-pyrimidin-5-yl}- butylsulfanyl}-2-methyl-phenoxy)-acetic acid
Al [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- butylsulfanyl}-2-methyl-phenoxy)-acetic acid tert-butyl ester 231 mg (0.7 mmol) cesium carbonate was added to a solution of 210 mg (0.59 mmol) [rac]-5-(1-chloro-butyl)-4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine (example 30D]) and 151 mg (0.59 mmol) (4-mercapto-2-methyl-phenoxy)-acetic acid tert-butyl ester (example 28C]) in 3 ml DME. After stirring for 3h at RT, the reaction mixture was taken up in ether and washed with IN HCl and water. The crude product was purified by chromatography over silica gel with ACOEt/heptane 1: 9 providing 257 mg of pure [rac]- (4-{1-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]- butylsulfanyl}-2-methyl-phenoxy)-acetic acid tert-butyl ester. oo MsspaMR).
B] [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-yi]- butylsulfanyl}-2-methyl-phenoxy)-acetic acid
A solution of 234 mg (0.44 mmol) [rac]-(4-{1-[4-cyclopropyl-2-(4-trifluoromethyl- phenyl)-pyrimidin-5-yl]-butylsulfanyl}-2-methyl-phenoxy)-acetic acid tert-butyl ester and 0.5 ml trifluoroacetic acid in 2.5 ml dichloromethane were stirred at RT for 4h. The solution was concentrated under reduced pressure and the crude product was repeatedly concentrated in vacuo with ether until a foam of 155 mg pure [rac]-(4-{1-[4- cyclopropyl-2-(4-triflucro-methyl-phenyl)-pyrimidin-5-yl] -butylsulfanyl}-2-methyl- phenoxy)-acetic acid resulted.
MS: 515.2 (M-H)", [rac)-5-(1-Chloro-butyl)-4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidine used in 30A] was synthesized as follows:
Cl [rac]-1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yi} -butan-1-ol
The synthesis was accomplished in the same way like 28C] and 28D}, with the difference that methylmagnesium bromide was replaced by propylmagnesium bromide. [rac]-1-[4- cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-butan-1-ol was received.
MS: 337.0 (M+H)".
D] [rac]-5-(1-Chloro-butyl)-4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidine
A solution of 392 mg (1.17 mmol) [rac]-1-[4-cyclopropyl-2-(4-triflucromethyl-phenyl)- pyrimidin-5-yl}-butan-1-ol and 0.09 ml (1.22 mmol) thionylchloride in 4 ml dichloromethane were stirred at RT for 1h. After concentration under reduced pressure 421 mg of pure [rac]-5-(1-chloro-butyl)-4-cyclopropyl-2-(4-trifiucromethyl-phenyl)- pyrimidine was isolated.
MS: 355.81 (M+H)™.
Example 31 [rac]-(4-{1-[4-Cyclopropyl-2-(4-triftuoromethyl-phenyl)-pyrimidin-5-yl}-butoxy}-2- methyl-phenoxy)-acetic acid
A] [racl-(4-{1-[4-Cyclopropyl-2-(4-trifilucromethyl-phenyl)-pyrimidin-5-yi}-butoxy}-2- methyl-phenoxy)-acetic acid tert-butyl ester 28 mg (0.0.642 mmol) sodium hydride (55% in oil) was added to a solution of 175 mg (0.49 mmol) [rac]-5-(1-chloro-butyl)-4-cyclopropyl-2-(4-trifluoromethyl-phenyl)- pyrimidine (example 30D]) and 104 mg (0.49 mmol) (4-hydroxy-2-methyl-phenoxy)- acetic acid ethyl ester (W002/092590) in 3 ml DMF. After stirring for 3h at RT, the reaction mixture was taken up in ether and washed with 1N HCl and water. The crude product was purified by chromatography over silica gel with AcOEt/heptane 1: 4 providing 165 mg of pure [rac]-(4-{1-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)- pyrimidin-5-yl]-butoxy}-2-methyl-phenoxy)-acetic acid tert-butyl ester.
MS: 529.0 (M+H)".
Bl [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-butoxy}-2- methyl-phenoxy)-acetic acid
A solution of 150 mg (0.261 mmol) [rac]-(4-{1-[4-cyclopropyl-2-(4-trifluoromethyl- phenyl)-pyrimidin-5-yl]-butoxy}-2-methyl-phenoxy)-acetic acid tert-butyl ester and 0.52 ml IN lithium hydroxide solution in 1.5 ml THE were stirred at RT for 2h. The solution was partitioned between ether, IN HCl and water and the ether layer then concentrated under reduced pressure. The crude product was repeatedly concentrated in with ether until a foam of 121 mg pure [rac]-(4-{1-[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)- pyrimidin-5-yl]-butoxy}-2-methyl-phenoxy)-acetic acid was obtained.
MS: 478.49 (M-H)",
Example 32 2-{4-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid
Al 2-{4-[4-Cydlopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2- methyl-phenoxyl-2-methyl-propionic acid ethyl ester
In analogy to the procedure described in example 29A], 2-(4-hydroxy-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) was reacted with 5-chloromethyl-4-cyclopropyl-2-(4-trifinoromethyl-phenyl)-pyrimidine (example 27F]) to give the title compound as colorless solid.
MS: 515.3 (M+H)".
B] 2-{4-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy}-2- methyl-phenoxy}-2-methyl-propionic acid
In analogy to the procedure described in.example 29B}, saponification of 2-{4-[4- cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy]-2-methyl-phenoxy}- 2-methyl-propionic acid ethyl ester yielded the title compound as white crystals of mp. 191.0-192.2 °C.
MS: 485.5 (M-H)'
Example 33 (2-Methyl-4-{methyl-[5-(4-trifluoromethyl-phenyl)-pyrazin-2-ylmethyl]-amino}- phenoxy)-acetic acid
Al (2-Methyl-4-{methyl-[5-(4-trifluoromethyl-phenyl)-pyrazin-2-ylmethyl|-amino}- phenoxy)-acetic acid methyl ester
A suspension of 53 mg (0.39 mmol) potasium carbonate and 52 mg (0.35 mmol) sodium iodide and 73 mg (0.35 mmol) (2-methyl-4-methylamino-phenoxy)-acetic acid methyl ester (example 5F) in 2 ml of DMSO was treated with 32 mg (0.12 mmol) of 2- chloromethyl-5-(4-trifluoromethyl-phenyl)-pyrazine (example 33E}). After 1.5h at RT, the second portion of 32 mg (0.12 mmol) of 2-chloromethyl-5-(4-trifluoromethyl- phenyl)-pyrazine was added. The third part-was added after 2h. The reaction mixture was stirred for total 19h at RT. It was then taken up in ether and washed with aqueous 10%
KHSO,-solution and water. The organic phase was washed with NaCl (10%), dried {Na;SO4) and evaporated. Purification by flash chromatography (SiO, heptane/AcOEt=9/1) gave 126 mg of pure (2-methyl-4-{methyl-{5-(4-trifluoromethyl- phenyl)-pyrazin-2-ylmethyl]-amino}-phenoxy)-acetic acid methyl ester.
MS: 446.3 (M+H)".
B] (2-Methyl-4-{ methyl-[5-(4-trifluoromethyl-phenyl)-pyrazin-2-yimethyl]l -amino}- phenoxy)-acetic acid
A solution of 110 mg (0.25 mmol) (2-methyl-4-{methyl-[5-(4-trifluoromethyl-phenyl)- pyrazin-2-ylmethyl]-amino}-phenoxy)-acetic acid methyl ester in 0.75 ml of THF and 0.75 ml ethanol was treated at 0 °C with 0.74 ml IN LiOH and stirred for 45 min at RT.
The reaction was extracted with with aqueous 10% KHSO, /Et,O (3x). The organic phase was washed with aqueous 10% NaCl, dried over Na,SO, and evaporated. The product was crystallized from ether to give pure (2-methyl-4-{methyl-[5-(4-trifluoromethyl- phenyl)-pyrazin-2-ylmethyl]-amino}-phenoxy)-acetic acid.
MS: 432.4 (M+H)". _ 2-Chloromethyl-5-(4-trifluoromethyl-phenyl)-pyrazine used in 33A] was synthesized as follows:
Cl] 5-(4-Trifluoromethyl-phenyl)-pyrazine-2-carboxylic acid methyl ester [Following a procedure of Ford, Alan; Sinn, Ekkehard; Woodward, Simon. Exploitation of differential reactivity of the carbon-chlorine bonds in 1,3-dichloroisoquinoline. Routes to new N,N-
chelate ligands and 1,3-disubstituted isoquinolines. Journal of the Chemical Society,
Perkin Transactions 1: Organic and Bio-Organic Chemistry (1997), (6), 927-934]
A solution of 5.18 g (30 mmol) methyl 5-chloropyrazine-2-carboxylate in 150 ml degassed DMF was treated with 3.47 g (0.1 mmol) tetrakis(triphenylphosphine)palladium and heated at 80 °C. 6.27 g (33 mmol) of 4- (trifluoromethyl)benzeneboronic acid and 14 66 g (45 mmol) of cesium carbonate were added and heated for 3.5h. The reaction was cooled to RT and extracted with H,O (0 °C)/Et,O (3x). The organic phases were washed with aqueous 10% NaCl, dried (NaSOy) and evaporated. Purification by flash-chromatography on silica gel (heptane/AcOEt 95:5 to 2:1) gave 2.3 g (27%) of 5-(4-trifluoromethyl-phenyl)-pyrazine-2-carboxylic acid methyl ester.
MS: 283 (M+H)".
D] [5-(4-Trifluoromethyl-phenyl)-pyrazin-2-yl]-methanol 0.585 g (2.07 mmol) of the above synthesized 5-(4-trifluoromethyl-phenyl)-pyrazine-2- carboxylic acid methyl ester in 10 ml of abs. THF was cooled down to -30 °C and reacted with 5.18 ml of DIBAL-H-solution (1.2 M in toluene, 3 eq.) for 10 min. The reaction was warmed up during 2.5h to 10 °C and stirred 1h at RT. Careful quenching at 0 °C with saturated NaCl solution, twofold extraction with ether, washing with brine, drying over sodium sulfate, and evaporation of the solvents left a crude product which was purified by flash chromatography (SiO, heptane/AcOEt = 4/1) to deliver finally 0.325 g of the title compound as yellow solid.
MS: 254.1 (M)".
E] 2-Chloromethyl-5-(4-trifluoromethyl-phenyl)-pyrazine 0.20 g (0.79 mmol) of the above prepared [5-(4-trifluoromethyl-phenyl)-pyrazin-2-yl}- methanol was dissolved in 8 ml of CHCl; and treated dropwise at 0 °C with 0.11 ml (2 eq.) of SOCL,. The reaction mixture was kept at 0 °C for 1h, at RT for 18 hand 6 h at 30 °C. Evaporation of the solvents and dissolution in ether and heptane with consecutive evaporation afforded 0.21 g of pure title compound as light brown solid.
MS: 272.1 (M, 1C1)".
Example 34 vl 2-Methyl-2-{2-methyl-4-[2-methyl-6- (4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy)- phenoxy}-propionic acid
Al 2-Methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ethoxy] -phenoxyl-propionic acid ethyl ester
To 0.119 g (0.499 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) and 0.150 g (0.525 mmol) of the above prepared (example IN]) 3-chloromethyl-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine, dissolved in 3.3 ml of abs. acetonitrile, was added 0.179 g of Cs,CO3 (0.549 mmol). The reaction was stirred over night at ambient temperature. Pouring onto crashed ice /
NH,Cl-solution, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO, hexane/AcOEt=8/2) yielded 0.221 g of pure title compound as colorless oil.
MS: 488.4 (M+H)".
Bl} 2-Methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ylmethoxy] -phenoxy}-propionic acid 0.221 g (0.453 mmol) of the above prepared 2-methyl-2-{2-methyl-4-[2-methyl-6-(4- trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]-phenoxy}-propionic acid ethyl ester was dissolved in 2.7 ml of THR/EtOH=1/1, treated with 1.35 ml (3 eq.) of IN NaOH and kept at ambient temperature for 4 h. The reaction mixture was then poured onto crashed ice/ AcOEt/HCI dil, the aqueous phase extracted again with AcOEt, the combined organic layers were washed with water, dried over sodium sulfate, and evaporated to dryness. Recrystallisation from AcOEt/hexane afforded finally 0.194 g of the title compound as white solid of mp. 164-165 °C.
MS: 458.2 (M-H)".
Example 35 ~. [racl-2-Methyl-22-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl butoxy}-phenoxy)-propionic acid
A] The title compound was prepared in analogy to example 34, via [rac]-2-methyl-2-(2- methyl-4-{1-[2-methyl-6-(4-trifluoromethyi-phenyl)-pyridin-3-yl]-butoxy}-phenoxy)- propionic acid ethyl ester, but using in step A] [rac]- 3-(1-chloro-butyl)-2-methyl-6-(4-
trifluoromethyl-phenyl)-pyridine (example 21B]) instead of 3-chloromethyl-2-methyl-6- (4-trifluoromethyl-phenyl)-pyridine, as colorless foam.
MS: 500.2 (M-H)"
Example 36
A) [rac]-2-Methyl-2-(2-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- yl]-propoxy}-phenoxy)-propionic acid
The title compound was prepared in analogy to example 34, via [rac]-2-methyl-2-(2- methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-propoxy}-phenoxy)- propionic acid, but using in step A] [rac]-3-(1-chloro-propyl)-2-methyl-6-(4- trifluoromethyl-phenyl)-pyridine (example 36 B]) instead of 3-chloromethyl-2-methyl- 6-(4-trifluoromethyl-phenyl)-pyridine, as colorless foam.
MS: 486.3 (M-H)". [rac]-3-(1-Chloro-propyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine used in 36A} was synthesized as follows:
B] [rac]-3-(1-Chloro-propyl)-2-methyl-6-(4-trifluoromethyl-phenyl)-pyridine
Was prepared in analogy to example 17C], but using for the Grignard-reaction ethyl magnesium chloride instead of the methyl derivative, as colorless oil.
MS: 314.2, 316.2 (M+H)".
Example 37 [rac]-2-Methyl-2-(2-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi]- ethoxy}-phenoxy)-propionic acid
A] The title compound was prepared in analogy to example 34, via [rac]-2-methyl-2-(2- methyl-4-{1-{2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl}-ethoxy}-phenoxy)- propionic acid, but using in step A} [rac]-3-(1-chloro-ethyl)-2-methyl-6-(4- _ trifluoromethyl-phenyl)-pyridine (example 17C}) instead of 3-chloromethyl-2-methyl-6- " (4-trifluoromethyl-phenyl)-pyridine, as off-white foam.
MS: 472.1 (M-H)".
Example 38 2-{4-[6-(3-Fluoro-4-trifiuoromethyl-phenyl)-2-methyl-pyridin-3-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid
Al 2-{4-[6-(3-Fluoro-4-trifluoromethyl-phenyl)-2-methyl-pyridin-3-ylmethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid ethyl ester
To 0.139 g (0.583 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) and 0.177 g (0.583 mmol) of 3-chloromethyl-6- (3-fluoro-4-trifluoromethyl-phenyl)-2-methyl-pyridine (example 38C}), dissolved in 3 ml of abs. acetonitrile, was added 0.228 g of Cs;CO3 (0.700 mmol). The reaction mixture was stirred during 3 h at ambient temperature. Pouring onto crashed ice / NH,Cl- solution, twofold extraction with AcOEt, washing with water and brine, drying over magnesium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO2, hexane/AcOEt=88/12) yielded 0.219 g of pure title compound as white solid.
MS: 506.4 (M+H)".
Bl 2-{4-[6-(3-Fluoro-4-trifluoromethyl-phenyl)-2-methyl-pyridin-3-ylmethoxy]-2- methyl-phenoxy}-2-methyl-propionic acid 0.214 g (0.423 mmol) of the above prepared 2-{4-{6-(3-fluoro-4-trifiuoromethyl- phenyl)-2-methyl-pyridin-3-ylmethoxy] -2-methyl- phenoxy} -2-methyl-propionic acid ethyl ester was dissolved in 0.85 ml of THF/EtOH=1/1, treated with 1.27 ml (3 eq.) of IN
NaOH and kept at ambient temperature for 2 h. The reaction mixture was then poured onto crashed ice/AcOEt/HCI dil., the aqueous phase extracted again with AcOEt; the combined organic layers were washed with water and brine, dried over. magnesium sulfate, and evaporated to dryness. Recrystallisation from AcOEt/hexane yielded finally 0.191 g of the title compound as white crystals of mp. 175-76 °C.
MS: 476.1 (M-H)". 3-Chloromethyl-6-(3-fluoro-4-trifluoromethyl-phenyl)-2-methyl-pyridine used in 384) was synthesized as follows: a ~ Cl 3-Chloromethyl-6-(3-fluoro-4-trifluoromethyl-phenyl)-2-methyi-pyridine was prepared in analogy to example 24B], but starting the whole reaction sequence with 1-(3-fluoro-4-trifluoromethyl-phenyl)-ethanone instead of 1-(4-fluoro-3- trifluoromethyl-phenyl)-ethanone, as off-white solid.
MS: 303.1, 305.1 (M)*.
Example 39 2-Methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3- ylmethoxy]-phenoxy}-propionic acid
A] 2-Methyl-6-(4-trifluoromethoxy-phenyl)-nicotinic acid methyl ester was prepared in analogy to example 1L], but starting the whole reaction sequence with 4- (trifluoromethoxy) acetophenone instead of 4- (trifluoromethyl) acetophenone, as beige solid.
MS: 311.0 (M)*.
B] [2-Methyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3-yll-methanol 1.54 g (4.95 mmol) of the above synthesized 2-methyl-6-(4-trifluoromethoxy-phenyl)- nicotinic acid methyl ester in 25 ml of abs. THF was cooled down to -20 °C and reacted with 14.84 ml of DIBAL-H-solution (1.0 M in toluene, 3 eq.) for 0.5 h. Careful quenching with ice / HC dil., twofold extraction with AcOBt, washing with water, drying over sodium sulfate, and evaporation of the solvents left a crude product which was purified by flash chromatography (SiOz, hexane/AcOEt=1/1) to deliver finally 0.877 g of the title compound as off-white solid of mp. 68-69 °C.
MS: 283.0 (M)".
Cl 3-Chloromethyl-2-methyl-6-(4-trifluoromethoxy-phenyl)-pyridine 0.300 g (1.059 mmol) of the above prepared [2-methyl-6-(4-trifluoromethoxy-phenyl)- pyridin-3-yl]-methanol was dissolved in 5.3 ml of CH,Cl; and treated dropwise at 0°C with 0.15 ml (2 eq.) of SOC. The reaction mixture was kept at 0 °C for 5 Min. and at RT for 30 Min. Pouring onto crashed ice / NaHCO3, twofold extraction with AcOEt, washing with water, drying over sodium sulfate and evaporation of the solvents generated 0.315 g of pure title compound as light yellow oil.
Co. MS:301.0,303.0 (MM). ee ee a
D] 2-Methyl-2-{2-methyl-4-[2-methyl-6-(4-triflucromethoxy-phenyl)-pyridin-3- ylmethoxy] -phenoxy}-propionic acid ethyl ester
To 0.123 g (0.516 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) and 0.163 g (0.540 mmol) of the above prepared
3-chloromethyl-2-methyl-6-(4-triftuoromethoxy-phenyl)-pyridine, dissolved in 4.7 ml of abs. acetonitrile, was added 0.184 g of Cs;CO3 (0.565 mmol). The reaction was stirred © over night at ambient temperature. Pouring onto crashed ice / NH;Cl-solution, twofold : extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO2, hexane/AcOEt=8/2) yielded 0.243 g of pure title compound as colorless oil.
MS: 504.5 (M+H)*.
EB] 2-Methyl-2-{2-methyl-4-[2-methyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3- ylmethoxyl-phenoxyl-propionic acid 0.243 g (0.483 mmol) of the above prepared 2-methyl-2-{2-methyl-4-[2-methyl-6-(4- trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy] -phenoxy}-propionic acid ethy ester was dissolved in 2.9 ml of THF/EtOH=1/1, treated with 1.45 ml (3 eq.) of IN NaOH and kept at room temperature for 4 h. The reaction mixture was then poured onto crashed ice/AcOEt/HCI dil., the aqueous phase extracted again with AcOEt; the combined organic layers were washed with water, dried over sodium sulfate, and evaporated to dryness. Recrystallisation from AcOEt/hexane produced finally 0.221 g of the title compound as white solid of mp. 149-151 °C.
MS: 474.1 (M-H)".
Example 40 2-{4-[2-Cyclopropyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy}-2-methyl- phenoxy}-2-methyl-propionic acid
Al 3-Amino-3-cydopropyl-acrylic acid ethyl ester
To 6.54 g of activated (by successively washing with 2N HCI, water, EtOH, and ether) and dried Zn dust (100 mmol) in 60 ml of abs. BtOH was added 0.4 ml of ethyl bromoacetate and the mixture refluxed under Ar for 20 Min., whereby the color turned greenish. 1.342 g of cyclopropyl cyanide (20 mmol) was then added, followed by the remaining 8.47 ml of ethyl bromoacetate (in total 80 mmol), and refluxing continued for ... another.hour. The reaction mixture was cooled down, poured carefully onto crashed ice /
NaHCO, and filtrated over Celite. The aqueous phase was extracted once more with
AcOEt, the combined organic layers were washed with water and brine, dried over magnesium sulfate, and evaporated to dryness. Flash chromatography (SiO, hexane/AcOEt=8/2) yielded 3.21 g of material containing according to NMR and GC/MS mainly the desired title compound, besides some 3-cyclopropyl-3-oxo-propionic acid ethyl ester (product of hydrolysis) and 3-oxo-butyric acid ethyl ester (selfcondensation of the reagent); however, it was used as such.
MS: 155 (M)".
Bl 2-Cyclopropyl-6-(4-trifluoromethyl-phenyl)-nicotinic acid ethyl ester 0.657 g (2.33 mmol) of the above prepared (example 1L}) 3-dimethylamino-1-(4- trifluoromethyl-phenyl)-propan-1-one hydrochloride and 0.362 g (1.0 eq., not corrected for impurities) of the above synthesised 3-amino-3-cyclopropyl-acrylic acid ethyl ester was dissolved in 7 ml of AcOH and heated to reflux for 3h. After cooling, the bulk of the solvent was evaporated i. V. Ensuing flash chromatography (S10, hexane/AcOFEt=93/7) yielded 0.101 g of the title compound as light yellow solid.
MS: 336.2 (M+H)".
C].[2-Cyclopropyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-methanol 0.193 g (0.576 mmol) of the above prepared 2-cyclopropyl-6-(4-trifluoromethyl-phenyl)- nicotinic acid ethyl ester in 3.0 ml of abs. THF was cooled down to 0 °C and reacted with 1.5 ml of DIBAL-H-solution (1.0 M in toluene, 2.6 eq:) for 1 h. Careful quenching with ice, filtration over Celite, separation of the layers, reextraction of the aqueous phase with
AcOEt, washing of the combined organic layers with NH,Cl, and drying over magnesium sulfate was followed by evaporation of the solvents. The crude product was finally purified by flash chromatography (SiOz, hexane/AcOEt=7/3) to deliver 0.128 g of the title compound as white crystals.
MS: 293.1 (M)". .
D1 3-Chloromethyl-2-cyclopropyl-6-(4-triflucromethyl-phenyl)-pyridine 0.127 g (0.433 mmol) of the above prepared [2-cyclopropyl-6-(4-trifluoromethyl- phenyl)-pyridin-3-yl]-methanol was dissolved in 2.0 ml of CHCl; and treated at 0°C with 0.063 ml (2 eq.) of SOCl,. The reaction mixture was kept at 0 °C for 5 Min. and at
RT for 60 Min. Pouring onto crashed ice / NaHCO3, twofold extraction with AcOEt, washing with water and brine, drying over magnesium sulfate, and evaporation of the solvents afforded 0.137 g of pure title compound as off-white solid.
MS: 312.1, 314.0 (M+H)".
E] 2-{4-[2-Cyclopropyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]-2-methyl- henoxy}-2-methyl-propionic acid ethyl ester
To 0.102 g (0.428 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) and 0.134 g (0.429 mmol) of the above prepared 3-chloromethyl-2-cyclopropyl-6-(4-trifluoromethyl-phenyl)-pyridine, dissolved in 2.5 ml of abs. acetonitrile, was added 0.168 g of Cs;COs (0.516 mmol). The reaction was stirred over night at ambient temperature. Pouring onto crashed ice / NH,Cl-solution, twofold extraction with AcOEt, washing with water and brine, drying over magnesium sulfate, and evaporation of the solvents, followed by flash chromatography (S10, hexane/AcOEt= 88/12) yielded 0.221 g of pure title compound as colorless oil.
MS: 514.5 (M+H)".
F] 2-{4-[2-Cyclopropyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid 0.220 g (0.428 mmol) of the above prepared 2-{4-[2-cyclopropyl-6-(4-trifluoromethyl- phenyl)-pyridin-3-ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid ethyl ester was dissolved in 2.6 ml of THE/EtOH=1/1, treated with 1.30 ml (3 eq.) of IN NaOH and kept at room temperature for 2 h. The reaction mixture was then poured onto crashed ice/ ACOEt/HCI dil., the aqueous phase extracted again with AcOEt; the combined organic layers were washed with water and brine, dried over magnesium sulfate, and evaporated to dryness. Recrystallisation from AcOEt/hexane afforded eventually 0.198 g of the title compound as white crystals of mp. 170-171 °C.
MS: 484.2 (M-H)".
Example 41 . [rac]-2-Methyl-2-(2-methyl-4-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]- butoxy}-phenoxy)-propionic acid
A) The title compound was prepared in analogy to example 8, via [rac]-2-methyl-2-(2- methyl-4-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]-butoxy}-phenoxy)- .. . propionic-acid-ethyl ester, but using-in-step A] [rac]- 3-(1-chloro-butyl)-2-methyl-6-(3- trifluoromethyl-phenyl)-pyridine (example 4B}) instead of 3-chloromethyl-2-methyl-6- (3-triffluoromethyl-phenyl)-pyridine, as white foam.
MS: 500.2 (M-H)".
-06-
Example 42 2-{4-[4-Cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5-yimethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid
A) 4-Cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidine-5-carboxylic acid ethyl ester
To a solution of 0.953 g (4.24 mmol) commercially available 3-trifluoromethyl- benzamidine hydrochloride in 10 ml of ethanol was added 0.408 g (4.25 mmol) of sodium tert-butoxide. Two Min. later, 0.901 g (4.25 mmol) of crude (E,Z)-2- cyclopropane-carbonyl-3-ethoxy-acrylic acid methyl ester (example 27C], containing some Et-ester) was added and the reaction allowed to proceed over night at RT. The mixture was then poured onto crashed ice/AcOEt/HCI dil, the aqueous phase extracted again with AcOEt, the combined organic layers were washed with water, dried over sodium sulfate, and evaporated to dryness. Flash chromatography (SiO,, hexane/AcOEt= 9/1) yielded finally 1.253 g of title compound as white waxy solid (mixture of Me/Et- ester).
MS: 322.1,336.0(M)".
B]_[4-Cyclopropyl-2-(3-trifiluoromethyl-phenyl)-pyrimidin-5-yi]-methanol 1.250 g (3.717 mmol) of the above prepared 4-cyclopropyl-2-(3-trifluoromethyl-phenyl)- pyrimidine-5-carboxylic acid ethyl ester in 19ml of abs. THF was cooled down to -15 °C and reacted with 11.2 ml of DIBAL-H-solution (1.0 M in toluene, 3 eq.) for 1/2 h.
Careful quenching with ice / HCl dil., twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents left a crude product which was purified by flash chromatography (SiO, hexane/AcOEt=1/1) to yield finally 1.096 g of the title compound as white solid of mp. 108-09 °C.
MS: 294.1 (M)™.
CJ 5-Chloromethyl-4-cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidine : 0.300 g (1.019 mmol) of the above prepared [4~cyclopropyl-2-(3-trifluoromethyl- _ pheny})-pyrimidin-5-yl]-methanol was dissolved in 5 ml of CHCl, and treated at 0°C with 0.15 ml (2 eq.) of SOCI,. The reaction mixture was kept at 0 °C for 5 Min. and at ambient temperature for 30 Min. Pouring onto crashed ice / NaHCO3, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents gave 0.315 g of pure title compound as white solid of mp. 92-95 °C.
MS: 312.1, 314.0 (M)*.
D] 2-{4-[4-Cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimi idin-5-vimethoxy]-2- methyl-phenoxy!-2-methyl-propionic acid ethyl ester
To 0.114 g (0.478 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl-propionic acid ethyl ester (described in WO 02/092590) and 0.157 g (0.502 mmol) of the above prepared 5-chloromethyl-4-cyclopropyl-2-(3-triftuoromethyl-phenyl)-pyrimidine, dissolved in 4.4 ml of abs. acetonitrile, was added 0.171 g of Cs;CO3 (0.525 mmol). The reaction was stirred over night at ambient temperature. Pouring onto crashed ice / HCI dil.-solution, twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents, followed by flash chromatography (SiO, hexane/AcOEt= 8/2) yielded 0.240 g of pure title compound as white waxy solid.
MS: 515.4 (M+H)".
E] 2-{4-[4-Cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxyl-2- methyl-phenoxyt-2-methyl-propionic acid 0.240 g (0.466 mmol) of the above prepared 2-{4-[4-cyclopropyl-2-(3-trifiuoromethyl- phenyl)-pyrimidin-5-ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid ethyl ester was dissolved in 2.8 ml of THF/EtOH=1/1, treated with 1.40 ml (3 eq.) of IN NaOH and kept at ambient temperature for 7 h. The reaction mixture was then poured onto crashed ice/AcOEt/HCl dil., the aqueous phase extracted again with AcOEt; the combined organic layers were washed with water, dried over sodium sulfate, and evaporated to dryness. Recrystallisation from AcOEt/hexane afforded finally 0.196 g of the title compound as white solid of mp. 175-176 °C.
MS: 485.2 (M-H)".
Example 43 2-Methyl-2-(2-methyl-4-{2-[2-methy}-6-(4-trifluoromethyl-phenyl)-pyridin-3-yi}- ethoxy}-phenoxy)-propionic acid
Al] [2-Methyl-6-( 4-trifluoromethyl-phenyl )-pyridin-3-yl}-acetonitrile ....212gofthe above (example IN]) prepared 3-chloromethyl-2-methyl-6-(4- trifluoromethyl-phenyl)-pyridine (95.2 mmol) was dissolved in 100 ml of dimethyl sulfoxide; 5.9 g of sodium cyanide (120 mmol) was added and the mixture was stirred at room temperature for 18 hours. The reaction mixture was then poured onto a mixture of ice and water and was subsequently extracted with 3 portions of 400 ml of tert-butyl methyl ether. The combined organic layers were washed with water, then with brine, and dried over sodium sulfate. After evaporation of the solvents, 25.2 g of the title compound was obtained as pale yellow solid.
MS: 276.1 (M)".
B] [2-Methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-acetic acid
A mixture of 25 g (90 mmol) of the above prepared [2-methyl-6-(4-trifluoromethyl- phenyl)-pyridin-3-yl}-acetonitrile, 20 g of sodium hydroxide (500 mmol), 60 ml of water, and 250 ml! of propanol was stirred vigorously at 100 °C. Hydrolysis was complete after 2 hours. The reaction mixture was then evaporated to dryness and the residue was redissolved in 70 ml of water; 60 ml of cold 8 N aqueous HCl was then added and the acid extracted with three portions of 250 ml of ethyl acetate; the combined organic phases were washed with water and brine, dried over sodium sulfate, and evaporated to dryness.
Thereby, 25.1 g of the title product was obtained as pale yellow solid.
MS: 296.0 (M+H)".
C] [2-Methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yll-acetic acid methyl ester
A solution of 2.55 g (8.63 mmol) of the above prepared [2-methyl-6-(4-trifluoromethyl- phenyl)-pyridin-3-yl]-acetic acid in 25 ml of methanol was cooled to -10 °C; 1.88 ml (25.9 mmol) of thionyl chloride was added and the reaction mixture was then stirred at ambient temperature for 2 hours. Subsequently, the solution was poured onto ice water, then extracted with three portions of 50 ml of tert.-butyl methyl ether. The combined organic layers were washed with water, aqueous NaHCO;-solution and brine, and dried over sodium sulfate. After evaporation of the solvents, 2.60 g of the title compound was obtained as light brown solid.
MS: 309.1(M)™.
D] 2-[2-Methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yll-ethanol 2.60 g (8.40 mmol) of the above prepared [2-methyl-6-(4-trifluoromethyl-phenyi)- pyridin-3-yl]-acetic acid methyl ester, dissolved in 15 ml of dry tetrahydrofuran, was added under an argon atmosphere within 15 minutes to a stirred suspension of 0.38 g (10 mmol) of lithium aluminum hydride in 5 ml of tetrahydrofuran. The reaction was exothermic. Subsequently, the mixture was stirred at room temperature for 1 hour to complete the reduction. Then, 1 ml of ethyl acetate was added dropwise to destroy the excess of reagent, followed by water, drop after drop, under argon, with stirring and cooling. The reaction mixture was diluted with 50 ml of ethyl acetate, dried over sodium sulfate, and filtered. The filtrate was evaporated i. V. and the residue was purified by chromatography on SiO; with a mixture of dichloromethane and tert.-butyl methyl ether (4:1) as eluent. Thereby, 1.88 g of the title compound was obtained as white solid.
MS: 281.1 (M)".
E] 2-Methyl-2-(2-methyl-4-{2-[2-methyl-6-( 4-trifluoromethyl-phenyl)-pyridin-3-yi]- ethoxyl-phenoxy)-propionic acid ethyl ester 0.181 g (0.642 mmol) of the above prepared 2-(2-methyl-6-(4-trifluoromethyl-phenyl)- pyridin-3-yl]-ethanol and 0.153 g (0.642 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2- methyl-propionic acid ethyl ester (described in WO 02/092590) were dissolved in 8 ml of abs. THF and treated successively at 0° C with 0.156 g (0.771 mmol) of tributylphosphine and 0.133 g (0.770 mmol) of N,N,N’,N’-tetramethylazodicarboxamide. The cooling bath was then removed and stirring continued for one night. Filtration over a pad of Celite, evaporation of the solvent, followed by flash chromatography (SiO,, hexane/AcOEt= 85/15), delivered 0.070 g of the title compound as colorless oil.
MS: 502.4 (M+H)".
FJ] 2-Methyl-2-(2-methyl-4-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- ethoxy!}-phenoxy)-propionic acid 0.070 g (0.140 mmol) of the above prepared 2-methyl-2-(2-methyi-4-{2-[2-methyl-6-(4- trifluoromethyl-phenyl)-pyridin-3-yl]-ethoxy}-phenoxy)-propionic acid ethyl ester was dissolved in 0.84 ml of THF/EtOH=1/1, treated with 0.42 ml (3 eq.) of 1N NaOH and kept at ambient temperature for 4 h. The reaction mixture was then poured onto crashed ice/ AcOEt/HCI dil, the aqueous phase extracted again with AcOEt; the combined organic layers were washed with water, dried over sodium sulfate, and evaporated to dryness to yield 0.057 g of the title product as light yellow foam.
MS: 472.1 (M-H).
Example 44 2-Methyl-2-{2-methyl-4-[6-(4-trifluoromethyl-phenyl)-pyridazin-3-ylmethoxy]- phenoxy}-propionic acid
Al 6-(4-Trifluoromethyl-phenyl)-pyridazine-3-carboxylic acid methyl ester 0.400 g (2.32 mmol) of the known 6-chloro-pyridazine-3-carboxylic acid methyl ester was dissolved in 12 ml of abs. DMF and treated with 0.268 g (0.232 mmol) of Pd(PhsP),,
After heating to 80° C, 0.572 g (3.01 mmol) of 4-(trifluoromethyl)benzeneborinic acid and 1.133 g (3.48 mmol) of Cs;CO; was added and the reaction allowed to proceed for 3.5 h at the same temperature. After cooling, the mixture was poured onto crashed ice, extracted twice with AcOEt, washed with brine and water, and dried over sodium sulfate.
Evaporation of the solvents i. V., followed by flash chromatography (SiO, hexane/AcOEt= 1/1) delivered 0.316 g of a reddish solid which was recrystallised from hexane/AcOEt to remove remaining impurities. Thereby, 0.230 g of the title compound was isolated.
MS: 282.1 (M)*.
Bl [6-(4-Trifluoromethyl-phenyl)-pyridazin-3-yl]-methanol 0.582 g (2.062 mmol) of the above prepared 6-(4-trifluoromethyl-phenyl)-pyridazine-3- carboxylic acid methyl ester in 20 ml of abs. THF was cooled down to -15 °C and reacted with 5.16 ml of DIBAL-H-solution (1.0 M in toluene, 2.5 eq.) for 1/2 h. Careful quenching with ice / HCl dil., twofold extraction with AcOEt, washing with water, drying over sodium sulfate, and evaporation of the solvents left a crude product which was purified by flash chromatography (SiO, AcOEt) to yield 0.155 g of the title compound as light brown solid.
MS: 254.1 (M)™.
Cl 2-Methyl-2-{2-methyl-4-[6-(4-trifluoromethyl-phenyl)-pyridazin-3-ylmethoxyl- phenoxyl-propionic acid ethyl ester 0.170 g (0.669 mmol) of the above prepared [6-(4-trifluoromethyl-phenyl)-pyridazin-3- yl]-methanol and 0.163 g (0.684 mmol) of 2-(4-hydroxy-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester (described in WO 02/092590) were dissolved in 8 ml! of abs.
THF and treated successively at 0° C with 0.162 g (0.800 mmol) of tributylphosphine and 0.139 g (0.804 mmol) of N,N,N’,N’-tetramethylazodicarboxamide. The cooling bath was then removed and stirring continued for one night. The reaction mixture was then poured onto crashed ice, extracted twice with AcOEt, washed with water, and dried over sodium sulfate. Evaporation of the solvents i. V., followed by flash chromatography (8102, hexane/AcOEt= 7/3) yielded, after recrystallisation from hexane/AcOE, 0.208 g of a colorless solid.
MS: 475.4 (M+H)".
Claims (40)
1. Compounds of the formula Rr 6 RY R 0) x! R’ I oA o, s R R R and enantiomers and pharmaceutically acceptable salts and esters thereof, wherein X'isO,S, CH; Rlis hydrogen or C, ;-alkyl; R? is hydrogen or C, 7-alkyl, or, if X! is CH,, R? is hydrogen, C, 7-alkyl or C,.;-alkoxy; R’is hydrogen or C,_y-alkyl; R*and R® independently from each other are hydrogen, C, alkyl,
Cs.7-cycloalkyl, halogen, C,.7-alkoxy- C,.;-alkyl, Cp 7-alkenyl, C;.7-alkinyl, fluoro-C,.7-alkyl, cyano-C,_;-alkyl or cyano; R®, R® and R independently from each other are hydrogen, C, ;-alkyl,
Cs.7-cycloalkyl, halogen, C,.;-alkoxy- C;.;-alkyl, C,.7-alkenyl, C;.7-alkinyl, fluoro-C,;-alkyl, cyano-C;;-alkyl or cyano; and one of R>, R°and R’ is ve=Y. R' x2 ba (CR'ORM) ~ g wherein X?is S, 0, NR’, (CH2)pNR’CO, or (CH,)pCONR’, R’ is hydrogen, C;.7-alkyl, Cs.7-cycloalkyl, fluoro-C, ;-alkyl, hydroxy-C,.;-alkyl, or
Ci.7-alkoxy-C, 7-alkyl;
Y!, Y% Y® and Y* are Nor C-R*%, and 1 or 2 of Y', Y2, Y? and Y* are N and the other ones are C-R'%; R'is C,.7-alkyl, Cs 7-cycloalkyl, fluoro-C;-alkyl, or C;.7-alkoxy-C,.;-alkyl; R* is hydrogen, C,.s-alkyl, Cy.;-alkoxy-C;.;-alkyl; R'? independently from each other in each occurance is selected from hydrogen,
C,.7-alkyl, Cs.s-cycloalkyl, fluoro-C,.,-alkyl, C,.7-alkoxy-C;.7-alkyl, hydroxy-C;.7-alkyl, Cir alkylthio-C, 7-alkyl, carboxy-C; y-alkoxy-C,.;-alkyl, carboxy, carboxy-Cj.7-alkyl, mono- or di-C;.7-alkyl-amino-C,.;-alkyl,
C,.7-alkanoyl-C, ;-alkyl, C,.5-alkenyl, and C;7-alkinyl; R" is aryl or heteroaryl; misOorl,nis0,1,20r3,and pis 0,10r2,and thesumof m,nandpis1,2,3 or 4 provided that compounds of formula I are excluded, wherein X' is O, R? and R? are hydrogen, RS is equal to y=. R' x2 A be — N\ ERR — (CHa), y-Y X?isOorS,and mis 0.
2. Compounds of formula I according to claim 1, wherein X* is NR, (CH2)sNR’CO or (CH,)»CONR’, R’is hydrogen, Cys-alkyl, Cs.;-cycloalkyl, fluoro-C; ;-alkyl, hydroxy-C;.7-alkyl, or
Cy.7-alkoxy-C,.7-alkyl; and p is 0, 1 or 2. Co 3. Compounds of formula I according to claims 1 or 2, wherein X* is NR’, and R° is - hydrogen, C;.7-alkyl, Cs.7-cycloalkyl, fluoro-C;z-alkyl, hydroxy-C,.;-alkyl, or C,.5- alkoxy-C,.;-alkyl.
4. Compounds of formula I according to claims 1 or 2, wherein
X? is (CH,)pNR’CO, or (CH.)?CONR’, R® is hydrogen, Ci7-alkyl, Cs.y-cycloalkyl, fluoro-C,.;-alkyl, hydroxy-C,.7-alkyl, or Ci7-alkoxy-Cp 7-alkyl; and p is 0, 1 or 2.
5. Compounds of formula I according to any one of claims 1 to 4, wherein Ris Cpg-alkyl.
6. Compounds of formula I according to claim 2, selected from the group consisting of (2-(3-methoxy-propyl)-4-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3- ylmethyl}-amino}-phenoxy)-acetic acid, [rac]-[2-methyl-4-(methyl-{1-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-yl]- butyl}-amino)-phenoxy]-acetic acid, (4-{[6-(4-chloro-phenyl)-pyridin-3-ylmethyl] -methyl-amino}-2-methyl-phenoxy)-acetic acid, 2-methyl-2-[3-(methyl-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] - acetyl}-amino)-phenoxy]-propionic acid, and (4-{[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylmethyl}-methyl- amino}-2-methyl-phenoxy)-acetic acid.
7. Compounds of formula I according to claim 4, selected from the group consisting of 2-methyl-2-(3-{methyl-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylmethyl]- carbamoyl} -phenoxy)-propionic acid, 2-{3-({2-[4~cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl] -acetyl}-methyl- amino)-phenoxy}-2-methyl-propionic acid, 2-methyl-2-[3-(methyl-{2-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]- acetyl}-amino)-5-trifluoromethyl-phenoxy]-propionic acid, 2-{4-({[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5-carbonyl}-amino}- methyl)-phenoxy]-2-methyl-propionic acid, and 2-methyl-2-(4-{[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-ylcarbamoyl] - methyl}-phenoxy)-propionic acid.
_.. 30__ __.
8.Compounds of formula I according to claim 4, selected from the group consisting of 2-[3-chloro-4-({[4-trifluoromethyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5- carbonyl]-amino}-methyl)-phenoxy}-2-methyl-propionic acid, ‘ 2-methyl-2-[4-({[4-trifluoromethyl-2-(4-trifluoromethyl-phenyl)-pyrimidine-5- carbonyl]-amino}-methyl)-phenoxy]-propionic acid; and
2-(4-{[4-cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-ylcarbamoyl] -methyl}- phenoxy)-2-methyl-propionic acid.
9. Compounds of formula I according to claim 1, wherein X' and X” are O and Ris Ci-y-alkyl.
10. Compounds of formula I according to claim 1, wherein X! and X* are O and R? and R’ are Cy.7-alkyl. :
11. Compounds of formula I according to claim 10, selected from the group consisting of 2-methyl-2-{2-methyl-4-[2-methyl-6-(3-trifluoromethyl-phenyl)-pyridin-3-ylmethoxy]- phenoxy}-propionic acid, 2-methyl-2-{2-methyl-4-[6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy]- phenoxy}-propionic acid, and 2-{4-[4-cyclopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-yimethoxy}-2-methyl- phenoxy}-2-methyl-propionic acid.
12. Compounds of formula I according to claim 10, selected from the group consisting of 2-methyl-2-{2-methyl-4- (2-methyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3- . ylmethoxy]-phenoxy}-propionic acid, 2-{4-[4-cyclopropyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5-ylmethoxy] -2-methyl- phenoxy}-2-methyl-propionic acid, and 2-{4-[4-(2-methoxy-ethyl)-6-methyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5- ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid.
13. Compounds of formula I according to claim 10, selected from the group consisting of 2-{4-[2-cyclopropyl-6-(4-trifluoromethoxy-phenyl)-pyridin-3-ylmethoxy]-2-methyl- phenoxy}-2-methyl-propionic acid, 2-{4-[4-methoxymethyl-6-methyl-2-(3-trifluoromethyl-phenyl)-pyrimidin-5- ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2-cyclopropyl-6-(4-trifiuoromethoxy-phenyl)-4-trifluoromethyl-pyridin-3-
_..30.. . ylmethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, So 2-{4-[4-methoxymethyl-2-(4-trifluoromethoxy-phenyl)-pyrimidin-5-ylmethoxy] -2- methyl-phenoxy}-2-methyl-propionic acid, 2-{4-[2~cyclopropyl-6-(4-trifluoromethoxy-phenyl)-4-trifluoromethyl-pyridin-3- yimethoxy]-2-methyl-phenoxy}-2-methyl-propionic acid, and
2-{4-[4-methoxymethyl-2-(4-trifluoromethoxy-phenyl)-pyrimidin-5-ylmethoxy]-2- methyl-phenoxy}-2-methyl-propionic acid.
14. Compounds of formula I according to claim 1, wherein X'and X*are O and m is 1.
15. Compounds of formula I according to claim 14, selected from the group consisting of [rac)-(4-{1-[4-Cydlopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-ethoxy}-2- methyl-phenoxy)-acetic acid, and [rac]-(4-{1-[4-Cyclopropyl-2-(4-trifluoromethyl-phenyl)-pyrimidin-5-yl]-butoxy}-2- methyl-phenoxy)-acetic acid.
16. Compounds of formula I according to any one of claims 1 to 5, wherein X' is S.
17. Compounds of formula I according to claim 16, selected from the group consisting of [rac)-(2-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl] -ethoxy}- phenylsulfanyl)-acetic acid, and [rac]-(2-methyl-4-{1-(2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl]-butoxy}- phenylsulfanyl)-acetic acid. '
18. Compounds of formula I according to claim 1, wherein X' is O, X?is S and m is
1.
19. Compounds of formula I according to claim 18, selected from the group consisting of [rac]-(2-methyl-4-{1-[2-methyl-6-(4-trifluoromethyl-phenyl)-pyridin-3-yl}- ethylsulfanyl}-phenoxy)-acetic acid, and [rac]-(4-{1-[4-Cydopropyl-2-(4-trifiuoromethyl-phenyl)-pyrimidin-5-yl]- ethylsulfanyl}-2-methyl-phenoxy)-acetic acid.
20. Compounds of formula I according to any one of claims 1 to 19, wherein R? is unsubstituted phenyl or phenyl substituted with one to three groups selected from C.;- ) alkyl, C,.7-alkoxy, halogen, fluoro-C, ;-alkyl, fluoro-C, ;-alkoxy and cyano.
21. Compounds of formula I according to any one of claims 1 to 19, wherein R" is unsubstituted phenyl or phenyl substituted with one to three groups selected from C; 5- alkyl, C,.y-alkoxy, halogen, fluoro-C;.;-alkyl and cyano.
22. Compounds of formula I according to claim 21, wherein R" is phenyl substituted with halogen or fluoro-C;_ s-alkyl.
23. Compounds of formula I according to any one of claims 1 to 20, wherein R13 is phenyl substituted with fluoro-C, ;-alkoxy.
24. Compounds of formula I according to any one of claims 1 to 23, wherein R'is hydrogen.
25. Compounds of formula I according to any one claim 1, wherein R’ is C,.5-alkyl.
26. Compounds of formula I according to claim 1, wherein 2 of Y, YL Vand Y* are N and the other ones are C-R'%.
27. A process for the manufacture of compounds according to any one of claims 1 to 26, which process comprises a) reacting a compound of formula RS R? R® 0 x! Py a 1 oA , KF R” R wherein R' is C, ;-alkyl, R? to R® are as defined as in claim 1 and one of R®, R%r R’ is selected from -OH, -SH or -NHR’, wherein R® is as defined in claim 1, with a compound of formula 1 : Y=Y Rp? R'4 ba m — — Hae 5 (CROR™), wherein Y* to Y*, R'%, R™, R", m and n are as defined in claim 1 and R* is ~OH, -Cl, -Br, -I or another leaving group, to obtain a compound of formula i. : Ce mae emo em eme eee as R® FE R® Lo . 0 x! rR I-1 1 R~ ed R® rR" R’
wherein one of R®, R® and R’ is y=. rR" x A Va ~~ _(CHp™ Ne-Y (CRYORM),_ and wherein X?is O, S or -NR’, R! is Cy.;-alkyl and X}, Y to Y*, R”* to R”” and m and n are as defined in claim 1, and optionally hydrolysing the ester group to obtain a compound of formula I, wherein R! is hydrogen; or, alternatively, b) reacting a compound of formula Re 6 R R 0 xt R’ v 1 R~ oo, R® RY R’ wherein R' is C,7-alkyl, R? to R® are as defined as in claim 1 and one of R®, R%r R is —(CH,),-NHR’, wherein R’ and p are as defined in claim 1, with a compound of formula 1 ve=Y Rr" 0 A nr Vv \ CH - HO GRIORY), — CH, a-Y! wherein Y to YY, R', R', R", m and n are as defined in claim 1, to obtain a compound of formula " ee RR RE : 0) x! rR’ I-2 1 R~ XK R® rR” FR wherein one of R®, R® and R’ is ve=Y 13 2 _ rr IN _ (CH), \ \vo-Y* (CR1R™M),, and wherein X? is -(CHz),-NR’CO-, R' is Cy.7-alkyl and X,Y to Y, R* to R” and m,n and p are as defined in claim 1, and optionally hydrolysing the ester group to obtain a compound of formula I, wherein R! is hydrogen; or, alternatively, ¢) reacting a compound of formula Rr 4 6 RW ~ R 0 xXx R’ vi ,1 R~ oA R® RF R wherein R! is Cy.;-alkyl, R? to R® are as defined as in claim 1 and one of R’, Rr R'is —(CH,),-COOH, and p is defined as in claim 1, with a compound of formula 1 ye=Y 13 9 R X CH A a vn HN (CRr1OR1)_— ( 2n Y- wherein Y! to Y4, R%, RY, R*, R?®, m and n are as defined in claim 1, to obtain a compound of formula RS R mm eo - RY . r® . . I. 1 1-3 : 0 Xx R’ wd RZ R’
PCT/EP2004/012217 wherein or 2 of R®, R® and R is
1 . ye=Y 13 2 R oo _ (CH), NY (CRICR1) and wherein X? is -(CH,),-CONR’, R' is C;-alkyl and X', Y to Y*, R* to R® and m, n and p are as defined in claim 1, and optionally hydrolysing the ester group to obtain a compound of formula I, wherein R' is hydrogen.
28. Compounds according to any one of claims 1 to 26 when manufactured by a process according to claim 27.
29. Pharmaceutical compositions comprising a compound according to any one of claims 1 to 26 as well as a pharmaceutically acceptable carrier and/or adjuvant.
30. Pharmaceutical compositions according to claim 29 for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPARa agonists.
31. Compounds according to any one of claims 1 to 26 for use as therapeutically active substances.
32. Compounds according to any one of claims 1 to 26 for use as therapeutically active substances for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPARa agonists.
33. The use of compounds according to any one of claims 1 to 26 for the preparation of medicaments for the treatment and/or prevention of diseases which are modulated by PPARS and/or PPAR agonists.
34. The use according to claim 33 for the treatment and/or prevention of diabetes, non-insulin dependent diabetes mellitus, increased lipid and cholesterol levels, particularly low HDL-cholesterol, high LDL-cholesterol, or high triglyceride levels, atherosclerotic diseases, metabolic syndrome (syndrome X), obesity, elevated blood pressure, endothelial dysfunction, procoagulant state, dyslipidemia, polycystic ovary syndrome, inflammatory diseases, and proliferative diseases. AMENDED SHEET al
‘ PCT/EP2004/012217
35. The use according to claim 34 for the treatment and/or prevention of low HDL cholesterol levels, high LDL cholesterol levels, high triglyceride levels, and the metabolic syndrome (syndrome X).
36. A compound according to any one of claims 1 to 26, 28, 31 or 32, substantially as herein described with reference to and as illustrated in any of the examples.
37. A process according to claim 27, substantially as herein described with reference to and as illustrated in any of the examples.
38. A composition according to claim 29 or claim 30, substantially as herein described with reference to and as illustrated in any of the examples.
39. Use according to any one of claims 33 to 35, substantially as herein described with reference to and as illustrated in any of the examples.
40. The novel compounds, processes and the use of such compounds substantially as described herein before. AMENDED SHEET
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- 2006-04-28 CO CO06040755A patent/CO5690584A2/en active IP Right Grant
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