ZA200508820B - Method of quantifying the surfactant of a protein preparation - Google Patents

Method of quantifying the surfactant of a protein preparation

Info

Publication number
ZA200508820B
ZA200508820B ZA200508820A ZA200508820A ZA200508820B ZA 200508820 B ZA200508820 B ZA 200508820B ZA 200508820 A ZA200508820 A ZA 200508820A ZA 200508820 A ZA200508820 A ZA 200508820A ZA 200508820 B ZA200508820 B ZA 200508820B
Authority
ZA
South Africa
Prior art keywords
liquid
surfactant
phase
preparation
complex
Prior art date
Application number
ZA200508820A
Inventor
Morton Philip Harvey
Cameron Jason
Original Assignee
Delta Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Delta Biotechnology Ltd filed Critical Delta Biotechnology Ltd
Publication of ZA200508820B publication Critical patent/ZA200508820B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Food Science & Technology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention provides a method for removing a proteinaceous component from a liquid-phase surfactant preparation comprising (a) providing a liquid-phase surfactant preparation containing a proteinaceous component; (b) adding a complexing agent to the preparation of step (a) and allowing the complexing agent to form a complex with the surfactant; (c) simultaneously with step (b), or subsequently, adding a miscible precipitating agent to the preparation of step (a) or the product of step (b), io respectively, to form a liquid-phase reaction mixture and allowing the miscible precipitating agent to precipitate the proteinaceous component within the liquid-phase reaction mixture; and (d) separating the said complex from the precipitated proteinaceous component in the product of step (c) to provide a purified liquid-phase surfactant preparation; wherein the complex remains in solution within the liquid-phase reaction mixture, and wherein step (d) retains the complex in the liquid phase.
ZA200508820A 2003-05-07 2004-05-07 Method of quantifying the surfactant of a protein preparation ZA200508820B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0310347.0A GB0310347D0 (en) 2003-05-07 2003-05-07 Assay

Publications (1)

Publication Number Publication Date
ZA200508820B true ZA200508820B (en) 2007-04-25

Family

ID=9957504

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200508820A ZA200508820B (en) 2003-05-07 2004-05-07 Method of quantifying the surfactant of a protein preparation

Country Status (14)

Country Link
US (1) US20070082004A1 (en)
EP (1) EP1620718B1 (en)
JP (1) JP2007537971A (en)
KR (1) KR20060012281A (en)
CN (1) CN1816741A (en)
AT (1) ATE412890T1 (en)
AU (1) AU2004236021A1 (en)
CA (1) CA2524630A1 (en)
DE (1) DE602004017448D1 (en)
ES (1) ES2316984T3 (en)
GB (2) GB0310347D0 (en)
NZ (1) NZ543370A (en)
WO (1) WO2004099234A2 (en)
ZA (1) ZA200508820B (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013537971A (en) * 2010-09-13 2013-10-07 アッヴィ・インコーポレイテッド Highly sensitive monoclonal antibody residue detection assay
EP2681553B1 (en) * 2011-03-04 2017-05-10 Baxalta GmbH Method for the determination of polysorbate
CN103649111B (en) 2011-07-05 2018-03-13 阿尔布梅迪克斯医疗公司 Albumin preparation and purposes
US20130203969A1 (en) * 2011-08-19 2013-08-08 Emd Millipore Corporation Use of small molecules in methods for purification of biomolecules
US9683935B2 (en) * 2012-06-18 2017-06-20 Nalco Company Method to measure surfactant in fluid
GB201318840D0 (en) * 2013-10-24 2013-12-11 Univ Leeds Method and device for protein preparation
CA3033737A1 (en) * 2016-08-15 2018-02-22 Genentech, Inc. Chromatography method for quantifying a non-ionic surfactant in a composition comprising the non-ionic surfactant and a polypeptide
JP6928084B2 (en) 2016-10-04 2021-09-01 アルブミディクス リミティド Use of recombinant yeast-derived serum albumin
WO2021242908A1 (en) * 2020-05-26 2021-12-02 Lonza Ltd Method of determining surfactant concentration in a protein sample
CN113514573B (en) * 2021-04-15 2023-06-06 澳美制药(苏州)有限公司 Method for detecting content of span 60
CN114235986A (en) * 2021-11-25 2022-03-25 浙江艾兰得生物科技有限公司 High performance liquid chromatography detection method for contents of vitamins B1, B2, B6, nicotinic acid and nicotinamide

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2435759A1 (en) * 1974-07-25 1976-02-12 Merck Patent Gmbh Colorimetric determination of nonionic surfactants on site - via the cobalt thiocyanate complex, which is extracted and reacted with 4((5-chloro-2-pyridyl)azo)-1,3-diamino benzene
DE3711776A1 (en) * 1987-04-08 1988-10-27 Huels Chemische Werke Ag USE OF N-POLYHYDROXYALKYL Fatty Acid Amides As Thickeners For Liquid Aqueous Surfactant Systems
US5389279A (en) * 1991-12-31 1995-02-14 Lever Brothers Company, Division Of Conopco, Inc. Compositions comprising nonionic glycolipid surfactants
WO2002086492A1 (en) * 2001-04-17 2002-10-31 Chugai Seiyaku Kabushiki Kaisha Method of quantifying surfactant

Also Published As

Publication number Publication date
NZ543370A (en) 2007-08-31
KR20060012281A (en) 2006-02-07
GB0310347D0 (en) 2003-06-11
WO2004099234A2 (en) 2004-11-18
GB0520797D0 (en) 2005-11-23
AU2004236021A1 (en) 2004-11-18
ES2316984T3 (en) 2009-04-16
DE602004017448D1 (en) 2008-12-11
WO2004099234A3 (en) 2005-03-03
GB2416169A (en) 2006-01-18
ATE412890T1 (en) 2008-11-15
JP2007537971A (en) 2007-12-27
CA2524630A1 (en) 2004-11-18
EP1620718B1 (en) 2008-10-29
CN1816741A (en) 2006-08-09
GB2416169B (en) 2007-10-17
US20070082004A1 (en) 2007-04-12
EP1620718A2 (en) 2006-02-01

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