ZA200504616B - Novel piperidine derivatives as modulators of chemokine receptor CCR5 - Google Patents

Description

Novel piperidine derivatives as modulators of chemokine receptor CCRS ] The present invention relates to heterocyclic derivatives having pharmaceutical activity, to processes for preparing such derivatives, to pharmaceutical compositions ‘ 5 comprising such derivatives and to the use of such derivatives as active therapeutic agents.

Pharmaceutically active piperidine derivatives are disclosed in WO001/87839, EP-Al- 1013276, WO00/08013, WO099/38514, W099/04794, WO00/76511, W000/76512,

WO000/76513, WO00/76514, WO00/76972, US 2002/0094989 and Bioorg. Med. Chem. Lett. 13 (2003) 119-123.

Chemokines are chemotactic cytokines that are released by a wide variety of cells to attract macrophages, T cells, eosinophils, basophils and neutrophils to sites of inflammation and also play a role in the maturation of cells of the immune system. Chemokines play an important role in immune and inflammatory responses in various diseases and disorders, including asthma and allergic diseases, as well as autoimmune pathologies such as rheumatoid arthritis and atherosclerosis. These small secreted molecules are a growing superfamily of 8-14 kDa proteins characterised by a conserved four cysteine motif. The chemokine superfamily can be divided into two main groups exhibiting characteristic structural motifs, the Cys-X-Cys (C-X-C, or a) and Cys-Cys (C-C, or B) families. These are distinguished on the basis of a single amino acid insertion between the NH-proximal pair of cysteine residues and sequence similarity.

The C-X-C chemokines include several potent chemoattractants and activators of neutrophils such as interleukin-8 (IL-8) and neutrophil-activating peptide 2 (NAP-2).

The C-C chemokines include potent chemoattractants of monocytes and lymphocytes but not neutrophils such as human monocyte chemotactic proteins 1-3 (MCP-1, MCP-2 and

MCP-3), RANTES (Regulated on Activation, Normal T Expressed and Secreted), eotaxin and the macrophage inflammatory proteins 1a and 1B (MIP-1a. and MIP-1p).

Studies have demonstrated that the actions of the chemokines are mediated by . subfamilies of G protein-coupled receptors, among which are the receptors designated CCR1,

CCR2, CCR2A, CCR2B, CCR3, CCR4, CCRS5, CCR6, CCR7, CCR8, CCR9, CCR10, ’ 30 CXCRI1, CXCR2, CXCR3 and CXCR4. These receptors represent good targets for drug development since agents which modulate these receptors would be useful in the treatment of disorders and diseases such as those mentioned above.

The CCRS receptor is expressed on T-lymphocytes, monocytes, macrophages, dendritic cells, microglia and other cell types. These detect and respond to several chemokines, principally “regulated on activation normal T-cell expressed and secreted” (RANTES), macrophage inflammatory proteins (MIP) MIP-1a and MIP-18 and monocyte . 5 chemoattractant protein-2 (MCP-2).

This results in the recruitment of cells of the immune system to sites of disease. In many diseases it is the cells expressing CCRS which contribute, directly or indirectly, to tissue damage. Consequently, inhibiting the recruitment of these cells is beneficial in a wide range of diseases.

CCRS is also a co-receptor for HIV-1 and other viruses, allowing these viruses to enter cells. Blocking the receptor with a CCR5 antagonist or inducing receptor internalisation with a CCRS agonist protects cells from viral infection.

The present invention provides a compound of formula (I):

R! Rr a 0) wherein:

A is absent or is (CHz),; © R'is Cp alkyl, C(O)NR'R!!, C(O);R"?, NRPC(O)R", NR¥C(O)NR'RY, NR3C(0),R", heterocyclyl, aryl or heteroaryl;

RR! RY, R!6 and R"® are hydrogen or Ci alkyl;

R', R12 R™ R' and RY are C, s alkyl (optionally substituted by halo, hydroxy, Cy. alkoxy,

C,.6 haloalkoxy, Cs. cycloalkyl (optionally substituted by halo), Cs.¢ cycloalkenyl, S(C;.4 alkyl), S(O)(C14 alkyl), S(0)(C1.4 alkyl), heteroaryl, aryl, heteroaryloxy or aryloxy), aryl, heteroaryl, Cs; cycloalkyl (optionally substituted by halo or C4 alkyl), C47 cycloalkyl fused to a phenyl ring, Cs.7 cycloalkenyl, or, heterocyclyl (itself optionally substituted by oxo, C(O)(C:- alkyl), S(O)x(C1.6 alkyl), halo or C14 alkyl); or RY, R2, R" and R"" can also be - hydrogen; or R' and RY, and/or R'¢ and R!” may join to form a 4-, 5- or 6-membered ring which ’ optionally includes a nitrogen, oxygen or sulphur atom, said ring being optionally substituted by Ci. alkyl, S(O)(Ci.s alkyl) or C(O)(C1.6 alkyl);

R?is Cy. alkyl, phenyl, heteroaryl or C.7 cycloalkyl;

R? is H or C4 alkyl;

R* is aryl, heteroaryl, Cy. alkyl or C39 cycloalkyl;

X is O or S(O); m and n are, independently, 0, 1, 2 or 3, provided m + nis 1 or more; aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, cyano, nitro, hydroxy, OC(O)NR®R?, NR¥R”, NR*C(O)R”, NR*C(O)NR"R”,

S(0),NR®R®, NR®'S(0),R%, C(O)NR¥®R®, COR’, NR¥CO,R™, S(O)R®, OS(O)%R®, C.. ¢ alkyl (optionally mono-substituted by S(O)R* or C(O)NR®'R*), Cz.6 alkenyl, C;.6 alkynyl,

Cs.10 cycloalkyl, Cy.¢ haloalkyl, Cis alkoxy(Ci.s)alkyl, Ci. alkoxy (optionally mono- substituted by CO.R®, C(O)NR*R%, cyano, heteroaryl or C(O)NHS(0):R*),

NHC(O)NHR®’, Cy haloalkoxy, phenyl, phenyl(C,.4)alkyl, phenoxy, phenylthio, phenylS(O), phenylS(O),, phenyl(C;.4)alkoxy, heteroaryl, heteroaryl(C,.s)alkyl, heteroaryloxy or heteroaryl(C.)alkoxy; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(Ci4 alkyl), S(OXCy.4 alkyl),

S(0)2(C14 alkyl), S(O),NH;, S(O),NH(C\.4 alkyl), S(0)2N(Ci.4 alkyl), cyano, C14 alkyl, C4 alkoxy, C(O)NH,, C(O)NH(C; 4 alkyl), C(O)N(Ci. alkyl);, CO:H, COz(C14 alkyl),

NHC(0O)(C1.4 alkyl), NHS(O)>(C.14 alkyl), CF; or OCF; unless otherwise stated heterocyclyl is optionally substituted by C,.s alkyl [optionally substituted by phenyl {which itself optionally substituted by halo, C;.4 alkyl, C4 alkoxy, cyano, nitro, CF3, OCF3, (C14 alkyl)C(O)NH, S(O);NHz, C14 alkylthio, S(O)(C4 alkyl) or S(0)x(Ci4 alkyl)} or heteroaryl {which itself optionally substituted by halo, C;4 alkyl, Ci4 alkoxy, cyano, nitro, CF3, (C14 alkyl)C(O)NH, S(O); NH, C,4 alkylthio, S(OX C14 alkyl) or

S(0)1(C,4 alkyl)} ], phenyl {optionally substituted by halo, C,4 alkyl, C,.4 alkoxy, cyano, nitro, CFs, OCF, (C14 alkyl)C(O)NH, S(0):NH;, C14 alkylthio, S(OXC1.4 alkyl) or S(0)z(C:. + alkyl)}, heteroaryl {optionally substituted by halo, C4 alkyl, C,4 alkoxy, cyano, nitro, CFs, (C4 alky)C(O)NH, S(O);NH;, C14 alkylthio, S(OXCi4 alkyl) or S(0)(Ci4 alkyl)},

S(O)NR*R*, C(O)R*, C(0)z(C,.6 alkyl) (such as tert-butoxycarbonyl), C(O)x(phenyl(Ci.. alkyl) (such as benzyloxycarbonyl), C(O)NHR*, S(O),R*, NHS(O),NHR*’, NHC(O)R™, . NHC(O)NHRY or NHS(0),R*, provided none of these last four substituents is linked to a ring nitrogen; : 30 Kk, 1, p and q are, independently, 0, 1 or 2;

R2, RZ, R* R* RY RZ R R¥ RY R¥ R’' and R* are, independently, hydrogen or Ci. alkyl;

rR. R%, RY, RS, R¥ RZ, rR RS, R®, R®, RY, rR? RY, RY, RY, RY, RY, R®, RY, RY,

R*?, R® R% R* and R®’ are, independently, Ci.¢ alkyl (optionally substituted by halo, hydroxy, Cy.¢ alkoxy, Cy.s haloalkoxy, Cs. cycloalkyl, Cs. cycloalkenyl, S(C;.4 alkyl),

S(O)(C,.4 alkyl), S(0)2(C1.4 alkyl), heteroaryl, phenyl, beteroaryloxy or phenyloxy), Cs.1 . 5 cycloalkyl, phenyl or heteroaryl; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(C,.4 alkyl),

S(O)(C1.4 alkyl), S(0)2(Ci.4 alkyl), S(0)2NH;, S(0):NH(C1.4 alkyl), S(0):N(Ci4 alkyl), cyano, Cj. alkyl, Cy. alkoxy, C(O)NHo, C(O)NH(C, 4 alkyl), C(O)N(C, 4 alkyl), CO-H,

CO(Ci14 alkyl), NHC(O)(C, 4 alkyl), NHS(0)z(C).4 alkyl), C(O)(C14 alkyl), CF; or OCF3;

R¥ RZ, R¥,R® RY R* RY, R¥ RY, RZ, RS, RS, R* RY, R%2 R®, R* and R% may additionally be hydrogen; or a pharmaceutically acceptable salt thereof or a solvate thereof.

Certain compounds of the present invention can exist in different isomeric forms (such as enantiomers, diastereomers, geometric isomers or tautomers). The present invention covers all such isomers and mixtures thereof in all proportions.

Suitable salts include acid addition salts such as a hydrochloride, hydrobromide, phosphate, acetate, fumarate, maleate, tartrate, citrate, oxalate, methanesulphonate or p- toluenesulphonate. In addition to these further examples of acid addition salts are succinate, glutarate or malonate.

The compounds of the invention may exist as solvates (such as hydrates) and the present invention covers all such solvates.

Alkyl groups and moieties are straight or branched chain and, for example, comprise one to six (such as one to four) carbon atoms. Alkyl is, for example, methyl, ethyl, n-propyl, iso-propyl, n-butyl, sec-butyl or tert-butyl. Methyl is sometimes abbreviated to Me hereinbelow.

Haloalky! includes CF3, and haloalkoxy includes CF.

Fluoroalkyl includes, for example, one to six, such as one to three, fluorine atoms, and . comprises, for example, a CF; group. Fluoroalkyl is, for example, CF3; or CH2CFs.

Cycloalkyl is, for example, cyclopropyl, cyclopentyl or cyclohexyl (such as : 30 cyclohexyl). Cycloalkenyl includes cyclopentenyl.

Heterocyclyl is, for example, piperidine, piperazine, pyrrolidine, azetidine, tetrahydrofuran, morpholine or thiomorpholine. Further examples of heterocyclyl are tetrahydropyran and tetrahydrothiopyran.

Aryl includes phenyl and naphthyl. In one aspect of the invention aryl is phenyl.

Heteroaryl is, for example, an aromatic 5 or 6 membered ring, optionally fused to one or more other rings, comprising at least one heteroatom selected from the group comprising nitrogen, oxygen and sulphur; or an N-oxide thereof, or an S-oxide or S-dioxide thereof. : 5 Heteroaryl is, for example, furyl, thienyl (also known as thiophenyl), pyrrolyl, thiazolyl, isothiazolyl, pyrazolyl, oxazolyl, isoxazolyl, imidazolyl, [1,2,4]-triazolyl, pyridinyl, pyrimidinyl, pyrazinyl, indolyl, benzo[b]furyl (also known as benzfuryl), benz[b]thienyl (also known as benzthieny! or benzthiophenyl), indazolyl, benzimidazolyl, benztriazolyl, benzoxazolyl, benzthiazolyl, 1,2,3-benzothiadiazolyl, an imidazopyridinyl (such as imidazo[1,2a]pyridinyl), thieno[3,2-b]pyridin-6-yl, 1,2,3-benzoxadiazolyl (also known as benzo[1,2,3]thiadiazolyl), 2,1,3-benzothiadiazolyl, benzofurazan (also known as 2,1,3- benzoxadiazolyl), quinoxalinyl, a pyrazolopyridine (for example 1H-pyrazolo[3,4- b]pyridinyl), quinolinyl, isoquinolinyl, a naphthyridiny! (for example [1,6]naphthyridinyl or [1,8]naphthyridinyl), a benzothiazinyl or dibenzothiophenyl (also known as dibenzothienyl); or an N-oxide thereof, or an S-oxide or S-dioxide thereof. A further example of heteroaryl is tetrazolyl.

Aryloxy includes phenoxy.

Heteroaryloxy includes pyridinyloxy and pyrimidinyloxy.

Phenyl(C, alkyl)alkyl is, for example, benzyl, 1-(phenyl)eth-1-yl or 1-(phenyl)eth-2- yl

Heteroaryl(C,.s alkyl)alkyl is, for example, pyridinylmethyl, pyrimidinylmethyl or 1- (pyridinyl)eth-2-yl.

Phenyl(C,.4 alkoxy) is, for example, benzyloxy or phenylCH(CH;3)O.

Heteroaryl(C 4 alkoxy) is, for example, pyridinylCH:0, pyrimidinylCH:0 or pyridinylCH(CH3)O.

Heteroaryl rings can carry various substituents including sulphonyl groups. A sulphonyl group on a heteroaryl ring can be a good leaving group (susceptible to nucleophilic . displacement) and examples of such situation are: 2-methanesulphonyl-pyridine and 2- or 4- methanesulphonyl-pyrimidine. The present invention covers compounds including a : 30 heteroaryl ring carrying a sulphonyl group which are sufficiently stable (non-reactive) to be isolated using the experimental procedures described.

In one particular aspect the present invention provides a compound of formula 43) wherein: A is absent or is (CHz)z; R! is Cys alkyl, C(O)NR'’R"!, C(OXR"2, NR"C(O)R",

NRC(O)NR'RY, NR'¥C(O),R", heterocyclyl, aryl or heteroaryl; R'®, R”>, R'*, R'® and R*® are hydrogen or C,.¢ alkyl; RR? R™ R! and RY are Cg alkyl (optionally substituted by halo, hydroxy, C1.¢ alkoxy, Ci haloalkoxy, Cs.s cycloalkyl (optionally substituted by halo),

Cs.s cycloalkenyl, S(C,.4 alkyl), S(O)(Ci4 alkyl), S(0)(C.4 alkyl), heteroaryl, aryl, : 5 heteroaryloxy or aryloxy), aryl, heteroaryl, Cs.7 cycloalkyl (optionally substituted by halo or

C1. alkyl), C47 cycloalkyl fused to a phenyl ring, Cs; cycloalkenyl, or, heterocyclyl (itself optionally substituted by oxo, C(O)(C) alkyl), S(O)(C,.s alkyl), halo or C,4 alkyl); or rR

R'%, Rand R" can also be hydrogen; or R'? and R", and/or R'® and R'’ may join to form a 4-, 5- or 6-membered ring which optionally includes a nitrogen, oxygen or sulphur atom, said ring being optionally substituted by Ci.¢ alkyl, S(O)(C.s alkyl) or C(O)(C,.6 alkyl); R?is C16 alkyl, phenyl, heteroaryl or Cs.; cycloalkyl; R? is H or C4 alkyl; R* is aryl, heteroaryl, Ci alkyl or Cs.7 cycloalkyl; X is O or S(O),; m and n are, independently, 0, 1, 2 or 3, provided m + nis 1 or more; aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, cyano, nitro, hydroxy, OC(O)NR*RY, NRZR”, NR*C(O)R”,

NR®C(O)NRPRZ, S(0),NR¥R, NR*'S(0),R*?, C(O)NR¥’R*, CO:R*, NR*’COR*,

S(0)R¥, 0S(0),R*, C.6 alkyl (optionally mono-substituted by S(O);R* or C(O)NR’'R™),

C,. alkenyl, C6 alkynyl, Cs.1o cycloalkyl, C,.¢ haloalkyl, Cy. alkoxy(Cy.¢)alkyl, Cy alkoxy,

C,.¢ haloalkoxy, phenyl, phenyl(C;)alkyl, phenoxy, phenylthio, phenylS(O), phenylS(O)s, phenyl(C;4)alkoxy, heteroaryl, heteroaryl(C;.4)alkyl, heteroaryloxy or heteroaryl(C4)alkoxy; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(C.4 alkyl), S(O)(Ci alkyl), S(0):(C, alkyl),

S(0):NH3, S(0);NH(C1.4 alkyl), S(0)2N(C4 alkyl), cyano, C4 alkyl, C,.4 alkoxy,

C(O)NH,, C(O)NH(C; alkyl), C(O)N(C.4 alkyl), CO-H, CO(Ci.4 alkyl), NHC(O)}Ci4 alkyl), NHS(O)(C,.4 alkyl), CF; or OCF3; unless otherwise stated heterocyclyl is optionally substituted by Cy. alkyl [optionally substituted by phenyl {which itself optionally substituted by halo, C,.4 alkyl, C4 alkoxy, cyano, nitro, CF3, OCF, (C14 alkyl)C(O)NH, S(O);NH,, C14 alkylthio, S(O)(C.4 alkyl) or S(0)2(C14 alkyl)} or heteroaryl {which itself optionally } substituted by halo, C4 alkyl, C,.4 alkoxy, cyano, nitro, CF3, (C14 alkyl)C(O)NH, S(0).NH,,

C1. alkylthio, S(O)(C1. alkyl) or S(0)2(Ci alkyl)}], phenyl {optionally substituted by halo, : 30 C4 alkyl, Ci4 alkoxy, cyano, nitro, CF3;, OCF3, (Ci4 alkyl)C(O)NH, S(O):NH,, Ci.4 alkylthio, S(O)(Ci alkyl) or S(0)2(C,4 alkyl)}, heteroaryl {optionally substituted by halo,

C,.4 alkyl, C14 alkoxy, cyano, nitro, CF3, (C4 alkyl)C(O)NH, S(O):NH,, C,.4 alkylthio,

S(0)(Ci4 alkyl) or S(O)(C\.4 alkyl)}, S(O):NR*R*, C(O)R*, C(O)(C, alkyl) (such as tert-butoxycarbonyl), C(O)z(pheny}(Ci-2 alkyl)) (such as benzyloxycarbonyl), C(O)NHR®,

S(0),R*, NHS(0);NHR*, NHC(O)R*, NHC(O)NHR"’ or NHS(O),R"*, provided none of these last four substituents is linked to a ring nitrogen; k, 1, p and q are, independently, 0, 1 or 2; R®, R%, R®, R*, rR? R%, R*, R%, RY, R* and R*! are, independently, hydrogen or Cy.¢ alkyl; R%, RZ, R®, R%, RY R¥, RY, R*, R® RY, RY, R®, R®, R¥, RY, R*, RY, R* RY,

R*® and R* are, independently, Ci.¢ alkyl (optionally substituted by halo, hydroxy, Ci.s alkoxy, C1.¢ haloalkoxy, Cs.s cycloalkyl, Cs. cycloalkenyl, S(Ci4 alkyl), S(O)(Ci« alkyl),

S(0)2(C 4 alkyl), heteroaryl, phenyl, heteroaryloxy or phenyloxy), Ci.; cycloalkyl, phenyl or heteroaryl; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(C1.4 alkyl), S(O)(C4 alkyl), S(0)2(Ci4 alkyl), S(0)2NH;, S(0);NH(C,.4 alkyl), S(O):N(C,. alkyl), cyano, C14 alkyl, Cy 4 alkoxy,

C(O)NHz, C(O)NH(C1 4 alkyl), C(O)N(C).4 alkyl), COzH, CO2(C14 alkyl), NHC(OXCi.4 alkyl), NHS(0)2(Ci4 alkyl), C(O)(Ci.4 alkyl), CF3 or OCF3;

R? R®, R®, RE RY R¥ RY, RR, RR, RY, R%, RY and R? may additionally be hydrogen; or a pharmaceutically acceptable salt thereof or a solvate thereof.

In a further aspect the present invention provides a compound of formula (I) wherein:

A is absent or is (CHa); R' is C15 alkyl, C(O)NR'’R'!, C(O);R'?, NR’C(O)R",

NRPC(O)NR'R", NR'®C(O),R", heterocyclyl (for example piperidine, piperazine, pyrrolidine or azetidine), aryl or heteroaryl; RR" RY,R'¢ and R'® are hydrogen or Cy. alkyl; RY, RZ RM" R' and R'® are C3 alkyl (optionally substituted by halo, hydroxy, Ci. alkoxy, Ci. haloalkoxy, Cs. cycloalkyl (optionally substituted by halo), Cs. cycloalkenyl,

S(C).4 alkyl), S(O)(Ci4 alkyl), S(0)2(Ci-4 alkyl), heteroaryl, aryl, heteroaryloxy or aryloxy), aryl, heteroaryl, C3.7 cycloalkyl (optionally substituted by halo or C;.4 alkyl), C47 cycloalkyl fused to a phenyl ring, Cs; cycloalkenyl, or, heterocyclyl (itself optionally substituted by oxo, C(0)(C.s alkyl), S(O)(C1.6 alkyl), halo or Ci. alkyl); or RY, R!2, R' and R!7 can also be hydrogen; or R'® and R'"', and/or R'® and R'” may join to form a 4-, 5- or 6-membered ring which optionally includes a nitrogen, oxygen or sulphur atom, said ring being optionally ] substituted by C6 alkyl, S(O)(C,.¢ alkyl) or C(O)(C1.s alkyD); R? is C6 alkyl, phenyl, heteroaryl or C;.7 cycloalkyl; R? is H or C14 alkyl; R* is aryl, heteroaryl, Cy. alkyl or C37 : 30 cycloalkyl; X is O or S(O),; m and n are, independently, 0, 1, 2 or 3, provided m +n is 1 or more; aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, cyano, nitro, hydroxy, OC(O)NR*R?, NR™R®, NR*C(O)RZ,

NR¥*C(O)NRRZ, S(O);NR®R®, NR! S(O)1R%, C(O)NR®R¥, COR, NRYCO,R%,

S(0);R*, 0S(0):R*, C, 6 alkyl (optionally mono-substituted by S(0),R* or C(O)NR®'R*?),

Ca. alkenyl, Cy. alkynyl, Cs.10 cycloalkyl, Cy.¢ haloalkyl, C,. alkoxy(Ci)alkyl, C,.¢ alkoxy,

C).¢ haloalkoxy, phenyl, phenyl(C,4)alkyl, phenoxy, phenylthio, phenylS(O), phenylS(O)., phenyl(C).4)alkoxy, heteroaryl, heteroaryl(C,.4)alkyl, heteroaryloxy or heteroaryl(C;.4)alkoxy; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(C14 alkyl), S(O)(C, 4 alkyl), S(O)2(C,s alkyl),

S(O); NH, S(0),NH(C; 4 alkyl), S(0)2N(C;.4 alkyl), cyano, C;4 alkyl, C4 alkoxy,

C(O)NH,, C(O)NH(C, 4 alkyl), C(O)N(C alkyl), COH, CO2(C) 4 alkyl), NHC(O)(Ci4 alkyl), NHS(0)2(C,.4 alkyl), CF; or OCFj3; unless otherwise stated heterocyclyl is optionally substituted by C,_¢ alkyl [optionally substituted by phenyl {which itself optionally substituted by halo, C}.4 alkyl, C;4 alkoxy, cyano, nitro, CFs, OCF, (C;.4 alkyl)C(O)NH, S(O),NH, C4 alkylthio, S(O)(Ci. alkyl) or S(0)2(Ci.4 alkyl)} or heteroaryl {which itself optionally substituted by halo, C14 alkyl, C14 alkoxy, cyano, nitro, CF3, (C4 alkyl)C(O)NH, S(O), NH,,

C4 alkylthio, S(O)(C, 4 alkyl) or S(O)x(C,.4 alkyl)}], phenyl {optionally substituted by halo,

C4 alkyl, C14 alkoxy, cyano, nitro, CF3, OCF3, (C;4 alkyl) C(O)NH, S(O);NH, Ci4 alkylthio, S(O)(C1.s alkyl) or S(0)z(Ci.4 alkyl)}, heteroaryl {optionally substituted by halo,

C1. alkyl, C,4 alkoxy, cyano, nitro, CF;, (C4 alkyl)C(O)NH, S(O):NH, C4 alkylthio,

S(0)(C1.4 alkyl) or S(O)(Ci.4 alkyl)}, S(O} NR¥R*, C(O)R*, C(0)2(C 16 alkyl) (such as tert-butoxycarbonyl), C(O)2(phenyl(C;.; alkyl)) (such as benzyloxycarbonyl), C(O)NHR®, S(0);R*, NHS(O),NHR*, NHC(O)R*, NHC(O)NHR* or NHS(O),R*, provided none of these last four substituents is linked to a ring nitrogen; k, 1, p and q are, independently, 0, 1 or 2: R® R2 R* R* RY,R%, R*, R¥® R* R* and R* are, independently, hydrogen or Ci. alkyl; R®, R®, R®, R®, R* R*, R* R*, R* RY, RY RZ R® R* R*,R% RY R® RY,

R*® and R* are, independently, C,.¢ alkyl (optionally substituted by halo, hydroxy, Ci.s alkoxy, Ci. haloalkoxy, Cs. cycloalkyl, Cs.s cycloalkenyl, S(Ci4 alkyl), S(O)(C;.4 alkyl),

S(0)2(C1.4 alkyl), heteroaryl, phenyl, heteroaryloxy or phenyloxy), Cs.7 cycloalkyl, phenyl or heteroaryl; wherein any of the immediately foregoing pheny! and heteroaryl moieties are ] optionally substituted with halo, hydroxy, nitro, S(C,4 alkyl), S(O)(Ci. alkyl), S(0)2(C14 alkyl), S(O);NH;, S(O): NH(C, 4 alkyl), S(0):N(C.4 alkyl),, cyano, C; 4 alkyl, Cy4 alkoxy, : 30 C(O)NH,, C(O)NH(C,4 alkyl), C(O)N(C,4 alkyl), CO-H, CO(C4 alkyl), NHC(OX(Ci4 alkyl), NHS(0)2(C.4 alkyl), C(O)(Ci alkyl), CF5 or OCF; R”, R®, R¥, R®, R¥, R*, R”,

R*, R*, R¥, R®, R*, R*, R* RY and R*? may additionally be hydrogen; or a pharmaceutically acceptable salt thereof or a solvate thereof.

In another aspect the present invention provides a compound of formula (I) wherein A is absent or is (CHz)z; R' is C15 alkyl, CO)NR'R"!, C(O)R'%, NR”C(O)R",

NRC(O)NRR'7, NR'®C(0),R", heterocyclyl (for example piperidine, piperazine, pyrrolidine or azetidine), aryl or heteroaryl; R'® RZ, R!® R!® and R"® are hydrogen or Cy . 5 alkyl; R!' RR" R! and R" are C3 alkyl (optionally substituted by halo, hydroxy, Ci. alkoxy, Cy. haloalkoxy, Cs cycloalkyl (optionally substituted by halo), Cs.¢ cycloalkenyl,

S(C14 alkyl), S(O)(Ci.4 alkyl), S(O)2(C1.4 alkyl), heteroaryl, aryl, heteroaryloxy or aryloxy), aryl, heteroaryl, Cs.; cycloalkyl (optionally substituted by halo or Ci.4 alkyl), C47 cycloalkyl fused to a phenyl ring, Cs.7 cycloalkenyl, or, heterocyclyl (itself optionally substituted by oxo,

C(O)Cus alkyl), S(O)(Cy.s alkyl), halo or Cy.4 alkyl); or R'', R'>, R' and R"’ can also be hydrogen; or R'® and R!!, and/or R'® and R'” may join to form a 4-, 5- or 6-membered ring which optionally includes a nitrogen, oxygen or sulphur atom, said ring being optionally substituted by Cj. alkyl, S(On(Ci.s alkyl) or C(O)(C;.¢ alkyl); R? C,. alkyl, phenyl, heteroaryl or C3; cycloalkyl; R® is H or C14 alkyl; R* is aryl or heteroaryl; X is O or S(O); m and n are, independently, 0, 1, 2 or 3, provided m + n is 1 or more, and provided that when X is O then m and n are not both 1; unless specified otherwise aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, cyano, nitro, hydroxy, OC(O)NR¥R?!, NRZR?, NR*C(O)R*, NR*C(O)NR*'R?, S(0),NR*R*,

NR?'S(0),R*, C(O)NR¥R*, CO,R*, NR¥'CO,R*, S(O)qR”, C16 alkyl, C2. alkenyl, Ca alkynyl, Cs.10 cycloalkyl, C; haloalkyl, C;. alkoxy(Ci.s)alkyl, Ci. alkoxy, C,.¢ haloalkoxy, phenyl, phenyl(C,)alkyl, phenoxy, phenylthio, phenylS(O), phenylS(O),, phenyl(C,. s)alkoxy, heteroaryl, heteroaryl(C;.4)alkyl, heteroaryloxy or heteroaryl(C;.4)alkoxy; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(C,.4 alkyl), S(O)(C1.4 alkyl), S(0)2(Ci.4 alkyl), S(O).NH, S(0)NH(C).4 alkyl), S(O)N(C1 alkyl), cyano, Ci alkyl, C4 alkoxy, C(O)NH,

C(O)NH(C,.4 alkyl), C(O)N(C.4 alkyl);, CO-H, CO(C14 alkyl), NHC(O)(C; 4 alkyl),

NHS(0)(C.4 alkyl), CF; or OCF; unless otherwise stated heterocyclyl is optionally substituted by C,.¢ alkyl [optionally substituted by phenyl {which itself optionally substituted by halo, C,4 alkyl, Ci. alkoxy, cyano, nitro, CFs, OCF3, (C;.4 alkyl)C(O)NH, S(O);NH;, C14 alkylthio, S(O)(Ci.s alkyl) or S(0),(C:.4 alkyl)} or heteroaryl {which itself optionally substituted by halo, Ci .4 alkyl, C4 alkoxy, cyano, nitro, CF3, (Ci4 alkyl)C(O)NH, S(O);NH,

C1. alkylthio, S(0)(C,4 alkyl) or S(0)(Ci4 alkyl)}], pheny! {optionally substituted by halo,

C4 alkyl, C;4 alkoxy, cyano, nitro, CF3, OCF3, (C14 alkyl)C(O)NH, S(O).NH,, C4 alkylthio, S(O)(C;.4 alkyl) or S(O)(Ci4 alkyl)}, heteroaryl {optionally substituted by halo,

C4 alkyl, C 4 alkoxy, cyano, nitro, CFs, (Ci.4 alkyl)C(O)NH, S(O);NHz, C14 alkylthio,

S(O)(C alkyl) or S(O)2(Ci4 alkyl)}, S(ONR*RY, C(O)R*™, C(0)2(Cr.6 alkyl) (such as tert-butoxycarbonyl), C(O).(phenyl(C1., alkyl)) (such as benzyloxycarbonyl), C(O)NHR®,

S(O)R*, NHS(O),NHR*, NHC(O)R*, NHC(O)NHR"’ or NHS(O),R*, provided none of these last four substituents is linked to a ring nitrogen; k, 1, p and q are, independently, 0, 1 or 2; R®, RZ, R*, R¥ RY, RY, R*, R®, R* and R® are, independently, hydrogen or Cy.¢ alkyl;

RZ R®, R¥ R®, R™ R¥, R*, R* RR”, RY, RZ, R® R™ R¥ R* R* and R*® are, independently, C,.¢ alkyl (optionally substituted by halo, hydroxy, C.¢ alkoxy, Ci. haloalkoxy, Cs.¢ cycloalkyl, Cs. cycloalkenyl, S(Cy4 alkyl), S(O)(C14 alkyl), S(0)x(Ci4 alkyl), heteroaryl, phenyl, heteroaryloxy or phenyloxy), Cs. cycloalkyl, phenyl or heteroaryl; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(C: 4 alkyl), S(O)(C,4 alkyl), S(O)(Ci.4 alkyl),

S(0);NH,, S(O),NH(C 4 alkyl), S(0)2N(C,.4 alkyl), cyano, C;4 alkyl, C;4 alkoxy,

C(O)NHz, C(O)NH(C;.4 alkyl), C(O)N(C14 alkyl);, CO:H, CO5(C14 alkyl), NHC(O)(C1.4 alkyl), NHS(O),(C14 alkyl), C(O)(Cy.4 alkyl), CF; or OCF3; R*, RZ, R”, R?, R*, R*, R¥,

R%, RY R* R® R* R*, R* and RY" may additionally be hydrogen; or a pharmaceutically acceptable salt thereof or a solvate thereof.

In a further aspect the present invention provides a compound of formula (I) wherein, unless specified otherwise, aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, hydroxy, nitro, S(C alkyl), S(O)(Ci.s alkyl), S(O)(Cis alkyl), S(0):NH;, S(O), NH(C,.¢ alkyl), S(O),N(C.¢ alkyl),, cyano, Cis alkyl, Cy alkoxy,

CH.S(0)2(C1 alkyl), OS(0)2(C,.6 alkyl), OCHyheteroaryl (such as OCHatetrazolyl),

OCH,CO-H, OCH,CO,(C, alkyl), OCH,C(O)NH;, OCH,C(O)NH(C,.s alkyl), OCH:CN,

NH,, NH(C).s alkyl), N(C1.s alkyl);, C(O)NH,, C(O)NH(C,.¢ alkyl), C(OIN(Cy alkyl),

C(O)[N-linked heterocyclyl], CO;H, CO(Ci.s alkyl), NHC(O)(C\.¢ alkyl), NHC(O)O(Ci-s alkyl), NHS(O)2(C1.s alkyl), CFs, CHF, CHyF, CH;CF3, OCF, phenyl, heteroaryl, phenyl(Ci- 4 alkyl), heteroaryl(C,.4 alkyl), NHC(O)phenyl, NHC(O)heteroaryl, NHC(O)(C;.4 alkyl)phenyl, NHC(O)(C,.4 alkyl)heteroaryl, NHS(O).phenyl, NHS(O), heteroaryl,

NHS(O)(Ci.4 alkyl)phenyl, NHS(O)x(C;.4 alkyl)heteroaryl, NHC(O)NH(C,.s alkyl),

NHC(O)NH(C3.7 cycloalkyl), NHC(O)NHphenyl, NHC(O)NHheteroaryl, NHC(O)NH(C, 4 alkyl)pheny! or NHC(O)NH(C,.4 alkyl)heteroaryl; wherein the foregoing phenyl and heteroaryl groups are optionally substituted by halo, hydroxy, nitro, S(C;4 alkyl), S(O)Ci4 alkyl), S(0)2(C1. alkyl), S(O):NH;, S(O).NH(C, 4 alkyl), S(0),N(C, 4 alkyl),, cyano, C4 alkyl, C14 alkoxy, C(O)NH,, C(O)NH(C}4 alkyl), C(O)N(C,4 alkyl), COH, CO»(Ci4 alkyl), NHC(O)(C,.4 alkyl), NHS(0)2(C14 alkyl), CF3 or OCF.

In yet another aspect the present invention provides a compound of formula (I) wherein, unless specified otherwise, aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, hydroxy, nitro, S(C.¢ alkyl), S(O)(Ci.s alkyl),

S(0)2(C1.6 alkyl), S(O), NH,, S(0)NH(C\. alkyl), S(O)2N(C,.s alkyl), cyano, Ci. alkyl, Cy alkoxy, NHz, NH(C,. alkyl), N(C. alkyl),, C(O)NH;, C(O)NH(C,.s alkyl), C(OIN(Ci.6 alkyl), C(O)[N-linked heterocyclyl], CO;H, CO2(Ci.s alkyl), NHC(O)(C,.s alkyl),

NHC(0)O(C, alkyl), NHS(O):(C1. alkyl), CFs, CHF, CHF, CH,CF3, OCF3, phenyl, heteroaryl, pheny}(C, 4 alkyl), heteroaryl(C,4 alkyl), NHC(O)phenyl, NHC(O)heteroaryl,

NHC(O)(C14 alkyl)phenyl, NHC(O)(C, 4 alkylheteroaryl, NHS(O),phenyl,

NHS(O); heteroaryl, NHS(O).(C4 alkyl)phenyl, NHS(O),(C; 4 alkyl)heteroaryl,

NHC(O)NH(C,.6 alkyl), NHC(O)NH(C;.; cycloalkyl), NHC(O)NHphenyl,

NHC(O)NHheteroaryl, NHC(O)NH(C;.4 alkyl)phenyl or NHC(O)NH(C,.4 alkyl)heteroaryl; wherein the foregoing phenyl and heteroaryl groups are optionally substituted by halo, hydroxy, nitro, S(C;.4 alkyl), S(OXC1.4 alkyl), S(0):(Ci-4 alkyl), S(O),NHz, S(0)2NH(C14 alkyl), S(0),N(C, alkyl), cyano, Cy4 alkyl, C,.4 alkoxy, C(O)NH;, C(O)NH(C, alkyl),

C(O)N(C,4 alkyl),, CO;H, CO2(C,4 alkyl), NHC(O)(C,.4 alkyl), NHS(0)2(C:.4 alkyl), CF; or

OCFs.

In a further aspect the present invention provides a compound of formula (I) wherein, unless specified otherwise, aryl, pheny! and heteroaryl moieties are independently optionally substituted by one or more of halo, hydroxy, nitro, S(Ci.s alkyl), S(O)(C..s alkyl), S(0)(Ci.6 alkyl), S(0);NH,, S(O); NH(C).s alkyl), S(0)N(C,.¢ alkyl), cyano, Cy alkyl, Ci.¢ alkoxy, CH3S(0)(C,.6 alkyl), OS(0)2(C1.¢ alkyl), OCHheteroaryl (such as OCHatetrazolyl),

OCH,CO,H, OCH,CO(C,. alkyl), OCH,C(O)NH;, OCH,C(O)NH(C,.¢ alkyl), OCH2CN,

NH,, NH(C,.¢ alkyl), N(C,_¢ alkyl),, C(O)NH;, C(O)NH(C,.s alkyl), C(O)N(C,.¢ alkyl),

CO,H, CO,(C).¢ alkyl), NHC(O)(C,.¢ alkyl), NHC(O)O(C,.s alkyl), NHS(0)2(C,.s alkyl),

CF;, CHF,, CH,F, CH,CF3, OCF;, heteroaryl or heteroaryl(Ci.4 alkyl); wherein the foregoing : 30 heteroaryl group (such as tetrazolyl) are optionally substituted by halo, hydroxy, nitro, S(Ci4 alkyl), S(O)(Ci.4 alkyl), S(O)2(C14 alkyl), S(O)2NHa, S(O),NH(C:4 alkyl), S(0)N(Ci4 alkyl), cyano, C;4 alkyl, C,4 alkoxy, C(O)NH,, C(O)NH(C4 alkyl), C(O)N(C,;4 alkyl)a,

CO,H, CO,(C, 4 alkyl), NHC(O)(C,4 alkyl), NHS(O),(C..4 alkyl), CF; or OCF; {and in a further aspect of the invention the foregoing heteroaryl groups (such as tetrazolyl) are optionally substituted by C;4 alkyl}.

In another aspect the present invention provides a compound of formula (I) wherein, unless specified otherwise, aryl, phenyl and heteroaryl moieties are independently optionally : 5 substituted by one or more of halo, hydroxy, nitro, S(C;.4 alkyl), S(O)(C1.4 alkyl), S(0)2(Ci.4 alkyl), S(O), NH,, S(0),NH(C. alkyl), S(O)N(Ci 4 alkyl), cyano, C14 alkyl, Ci. alkoxy,

C(O)NH;, C(O)NH(C; 4 alkyl), CO,H, CO»(C1.4 alkyl), NHC(O)(Ci.4 alkyl), NHS(0):(C;.4 alkyl), CFs, CHF, CH,F, CH,CF3 or OCF.

In a further aspect of the invention heteroaryl is tetrazolyl, pyrrolyl, thienyl, imidazolyl, thiazolyl, isoxazolyl, pyridinyl, pyrimidinyl, pyrazinyl or quinolinyl. Ina still further aspect heteroaryl is pyrrolyl, thienyl, imidazolyl, thiazolyl, isoxazolyl, pyridinyl, pyrimidinyl, pyrazinyl or quinolinyl.

In another aspect of the invention R!® R¥® RY Rand R!® are hydrogen or C4 alkyl (for example methyl). In yet another aspect R' RR", R! and R'® are hydrogen.

In a further aspect of the invention RY, R?, RM RY, R!® and R" are Cy. alkyl (optionally substituted by halo, C,.¢ alkoxy, Cis haloalkoxy, Cs cycloalkyl (optionally substituted by halo), Cs.s cycloalkenyl, S(O)x(C, 4 alkyl), heteroaryl, phenyl, heteroaryloxy or aryloxy (for example phenoxy)), phenyl, heteroaryl, C;.7 cycloalkyl (optionally substituted by halo or C;.4 alkyl), C4 cycloalkyl fused to a phenyl ring, Cs.7 cycloalkenyl, or, heterocyclyl (itself optionally substituted by oxo, C(O)}(Ci.¢ alkyl), S(O)(Ci-4 alkyl), halo or C4 alkyl); k is0, 1 or 2; or R'° and R!!, and/or R' and R"’ may join to form a 4-, 5- or 6-membered ring which optionally includes a nitrogen, oxygen or sulphur atom, said ring being optionally substituted by Cy.s alkyl or C(O)(Ci.s alkyl).

In yet another aspect of the invention R"!, R'2, R", R" and R" are Cy 5 alkyl (optionally substituted by halo (such as fluoro)), phenyl (optionally substituted as recited above), Cs. cycloalkyl (optionally substituted by halo (such as fluoro)) or C-linked nitrogen containing heterocyclyl (optionally substituted on the ring nitrogen).

In a further aspect R' is NHC(O)RY, phenyl or heterocyclyl, wherein Ris as defined above, and phenyl and heterocyclyl are optionally substituted as described above. : 30 In another aspect of the invention R' is NR**C(O)R', wherein R"* and R' are as defined above. For example R'? is hydrogen.

In yet another aspect of the invention R!* is C,.g alkyl (optionally substituted by halo (such as fluoro, for example to form CF3CHy)), phenyl (optionally substituted as recited above), Cs.¢ cycloalkyl (optionally substituted by halo (such as fluoro, for example to form 1,1-difluorocyclohex-4-y1)) or C-linked nitrogen containing heterocyclyl (such as tetrahydropyran or piperidine, optionally substituted on the ring nitrogen).

In another aspect the present invention provides a compound of the invention wherein

RMis C5 alkyl (optionally substituted by halo (such as fluoro, for example to form

CF3CHy)), phenyl (optionally substituted by halo) or Cs. cycloalkyl (optionally substituted by halo (such as fluoro, for example to form 1,1-difluorocyclohex-4-yl)).

In a further aspect of the invention heterocyclyl is optionally substituted (such as singly substituted for example on a ring nitrogen atom when present) by C,.¢ alkyl [optionally substituted by phenyl {which itself optionally substituted by halo, C,.4 alkyl, C14 alkoxy, cyano, nitro, CF3, OCF3, (Ci alkyl)C(O)NH, S(O),NH;, C14 alkylthio or S(0),(Ci.4 alkyl)} or heteroaryl {which itself optionally substituted by halo, C14 alkyl, C14 alkoxy, cyano, nitro,

CF, (C14 alkyl)C(O)NH, S(O);NH3, C,.4 alkylthio or S(0)2(Ci4 alkyD}], phenyl {optionally substituted by halo, C,_4 alkyl, C,.4 alkoxy, cyano, nitro, CF3, OCF3, (Ci4 alkyl)C(O)NH,

S(O)NH,, Ci alkylthio or S(O)(Ci.4 alkyl)}, heteroaryl {optionally substituted by halo, C4 alkyl, Cy alkoxy, cyano, nitro, CFs, (C14 alkyl)C(O)NH, S(O):NH;, C4 alkylthio or

S(0)x(Ci4 alkyD)}, S(OX;NR¥R*, C(O)R?, C(O)NHR™® or S(O),R*; wherein R*, RY, R¥,

R*® and R* are, independently, hydrogen or C; alkyl.

In yet another aspect of the invention Ris optionally substituted aryl (such as optionally substituted phenyl) or optionally substituted heteroaryl, wherein the optional substituents are as recited above.

In a further aspect of the invention when R! is heterocyclyl it is, for example, tetrahydropyran, tetrahydrothiopyran, piperidine, piperazine, pyrrolidine or azetidine. In another aspect when R! is heterocyclyl it is, for example, piperidine, piperazine, pyrrolidine or azetidine.

In a further aspect of the invention R! is optionally substituted heterocyclyl, such as optionally substituted: piperidin-1-yl, piperidin-4-yl, piperazin-1-yl, pyrrolidin-1-yl, pyrrolidin-3-yl, azetidin-1-yl or azetidin-3-yl.

In a still further aspect of the invention the heterocyclyl of R! is mono-substituted by : 30 Cus alkyl, C37 cycloalkyl, phenyl! {optionally substituted by halo (for example fluoro), Cia alkyl (for example methyl), C,.4 alkoxy (for example methoxy), CF; or OCF 3}, S(0)(Ci4 alkyl) (for example S(O),CHs, S(0),CH2CHj; or S(0),CH(CHa)z2), S(0)2(C14 fluoroalkyl) (for example S(0):CF; or S(0O).CH,CF3), S(O):phenyl {optionally substituted (such as mono-

substituted) by halo (for example chloro), cyano, C14 alkyl, C4 alkoxy, CFs, OCF;

S(0)2(C1.4 alkyl) (for example S(0),CHs or S(0).CH,CH,CH3) or S(0)2(Ci.4 fluoroalkyl) (for example S(0),CH,CF3)}, benzyl {optionally substituted by halo (for example chloro or fluoro), C14 alkyl, C14 alkoxy (for example methoxy), CF; or OCF;}, C(O)H, C(OXCi4 : 5 alkyl), benzoyl {optionally substituted by halo (for example chloro or fluoro), C4 alkyl (for example methyl), Cy alkoxy, CF; or OCF}, C(0)2(C1.4 alkyl), C(O)NH;, C(O)NH(Ci4 alkyl) or C(O)NHpheny! {optionally substituted by halo (for example fluoro), Cia alkyl, C4 alkoxy, CFs or OCF3}. Said heterocyclyl can also be mono-substituted by S(O):N(Ci.4 alkyl),. In a still further aspect when said heterocyclyl is a 4-substituted piperidin-1-yl, a 1- substituted piperidin-4-yl, a 4-substituted piperazin-1-yl, a 3-substituted pyrrolidin-1-yl, a 1- substituted pyrrolidin-3-yl, a 3-substituted azetidin-1-yl or a 1-substituted azetidin-3-yl (for example where said substituent is as recited earlier in this paragraph). In another aspect said heterocyclyl is a 1-substituted piperidin-4-yl or a 4-substituted piperazin-1-yl, wherein the substituent is S(O),(C}.4 alkyl), S(0)2(Ci4 haloalkyl), S(O)z(phenyl), S(0)2N(Ci.4 alkyl), or phenyl

In another aspect of the invention R! is piperidinyl or piperazinyl (such as piperidin-4- yl or piperazin-1-yl), either of which is N-substituted by phenyl, S(0),R* (wherein R* is C14 alkyl (such as methyl or ethyl), phenyl! or CF3) or S(O), NR*R*® (wherein R? and R* are, independently, C4 alkyl (such as methyl).

In yet another aspect of the invention R' is NHC(O)R'* wherein R'is Cy haloalkyl (for example C4 fluoroalkyl, such as CH,CF3; or CH2CH:CF3), phenyl (optionally substituted by halo) or Cs. cycloalkyl (substituted by one or two fluoros).

In a further aspect of the invention R! is phenyl optionally substituted by S(O},R¥ (wherein R* is Cy alkyl (such as methyl).

In a still further aspect of the invention R! is heteroaryl (such as pyridinyl) optionally substituted by CF.

In another aspect of the invention R! is heterocyclyl (such as tetrahydropyran or tetrahydrothiopyran).

In yet another aspect of the invention R? is phenyl or heteroaryl, either of which is : 30 optionally substituted by halo, Ci.4 alkyl, C14 alkoxy, S(O)a(Ci4 alkyl), nitro, cyano or CFs; wherein n is 0, 1 or 2, for example 0 or 2. When R? is heteroaryl it is, for example an optionally substituted thiophenyl (that is, thienyl).

Tn another aspect R? is phenyl or thienyl, either of which is optionally substituted by halo (such as chloro or fluoro) or CFs.

In a still further aspect R? is optionally substituted (for example unsubstituted or substituted in the 2-, 3-, or 3- and 5- positions) phenyl (such as optionally substituted by halo : 5 (such as chloro or fluoro), cyano, methyl, ethyl, methoxy, ethoxy or CFs), or optionally substituted (for example unsubstituted or mono-substituted) heteroaryl (such as optionally substituted by halo (such as chloro or fluoro), cyano, methyl, ethyl, methoxy, ethoxy or CF3).

In another aspect the invention provides a compound of the invention wherein R?is optionally substituted (for example unsubstituted or substituted in the 2-, 3-, or 3- and 5- positions) phenyl (such as optionally substituted by halo (for example chloro or fluoro)). In yet another aspect the invention provides a compound of the invention wherein R? is phenyl, 3-fluorophenyl, 3-chlorophenyl, 3-trifluoromethylphenyl, 3-chloro-5-fluoropbenyl or 3,5- difluorophenyl. In a further aspect the invention provides a compound of the invention wherein R? is phenyl, 3-fluorophenyl, 3-chlorophenyl or 3,5-difluorophenyl.

In yet another aspect of the invention R? is hydrogen or methyl. In a further aspect of the invention when R? is C14 alkyl (such as methyl) and the carbon to which R? is attached has the R absolute configuration. In yet another aspect of the invention R? is hydrogen.

In a still further aspect the present invention provides a compound of the invention wherein R* is optionally substituted phenyl (the optional substituents being selected from those recited above).

In another aspect the present invention provides a compound of the invention wherein

R* is optionally substituted aryl (such as phenyl) or optionally substituted heteroaryl (such as pyridyl, imidazolyl or 1,3,4-thiadiazolyl), (the optional substituents being selected from those recited above).

In yet another aspect the present invention provides a compound of the invention wherein R* is phenyl optionally substituted by one or more of halo, hydroxy, nitro, S(Ci.s alkyl), S(O)(Cs alkyl), S(0)2(Ci.¢ alkyl), S(0);NHz, S(O);NH(C\.¢ alkyl), S(0),N(Cy.6 alkyl),, cyano, C1. alkyl, Cy alkoxy, CH,S(0)2(C16 alkyl), 0OS(0),(Ci.6 alkyl),

OCH;heteroaryl (such as OCHatetrazolyl), OCH2CO2H, OCH,CO2(Cy6 alkyl),

OCH,C(O)NHa, OCH,C(O)NH(C, alkyl), OCH,CN, NH;, NH(C.6 alkyl), N(Cis alkyl),

C(O)NH,, C(O)NH(C;.6 alkyl), C(O)N(C;. alkyl),, CO.H, CO2(Ci alkyl), NHC(OXCis alkyl), NHC(O)O(C,.¢ alkyl), NHS(0)2(C,.s alkyl), CFs, CHF2, CHaF, CH,CF3, OCF;, heteroaryl or heteroaryl(Ci 4 alkyl); wherein the foregoing heteroaryl groups (such as tetrazolyl) are optionally substituted by halo, hydroxy, nitro, S(Ci4 alkyl), S(O)(C,.4 alkyl),

S(0)x(C1.4 alkyl), S(O),NHz, S(O);NH(C,.4 alkyl), S(0);N(C,.4 alkyl),, cyano, C4 alkyl, C4 alkoxy, C(O)NH,, C(O)NH(C;.4 alkyl), C(O)N(C,4 alkyl), COH, CO»(C14 alkyl),

NHC(O)(C;.4 alkyl), NHS(0)(Ci4 alkyl), CF; or OCF; {and in a further aspect of the invention the foregoing heteroaryl groups (such as tetrazolyl) are optionally substituted by C;. a alkyl}.

In a further aspect the present invention provides a compound of the invention wherein R? is phenyl optionally substituted by halogen (such as chloro or fluoro), cyano, C;.4 alkyl (mono-substituted by S(0)2(Ci4 alkyl) or C(O)NH(C, 4 alkyl), Ci4 alkoxy, S(Ci4 alkyl), S(0)x(Ci4 alkyl), OS(0)2(Ci.4 alkyl), OCH,COOH, OCH,-tetrazolyl (itself optionally substituted by C,.4 alkyl), carboxamide or tetrazolyl (itself optionally substituted by C;.4 alkyl).

In yet another aspect the present invention provides a compound of the invention wherein R* is aryl or heteroaryl each being optionally substituted by OS(O)R* or Cy alkyl (mono-substituted by S(0),R™ or C(O)NR*'R*?); wherein R*, R”’, R*! and R*’ are as defined above.

In a further aspect the present invention provides a compound of the invention wherein R* is phenyl (optionally substituted by halogen (such as chloro or fluoro), cyano, Cis alkyl, C14 alkoxy, S(Ci4 alkyl), S(0)2(C1.4 alkyl), OS(O)(Ci4 alkyl) or carboxamide), C37 cycloalkyl (such as cyclohexyl), pyridyl (optionally substituted by C,.4 alkyl), imidazolyl (optionally substituted by C;.4 alkyl) or 1,3,4-thiadiazolyl (optionally substituted by C14 alkyl).

In a further aspect the present invention provides a compound of the invention wherein R* is phenyl {optionally substituted by S(0)2(C;.4 alkyl) (such as CH3S(0), for example in the 4-position), C1.4 alkoxy (such as CH30, for example in the 4-position),

OS(0)2(Ci14 alkyl) (such as OSO,CHs, for example in the 4-position), halogen (such as chloro or fluoro) or cyano}.

In a still further aspect the invention provides a compound of the invention wherein A is absent.

In another aspect the invention provides a compound of the invention wherein X is O or S(O),. In yet another aspect X is S(O).

In a further aspect the invention provides a compound of the invention wherein m is 2 andnisOornis2and mis 0.

In a still further aspect the invention provides a compound of the invention wherein p 1s 0.

In another aspect the invention provides a compound of the invention wherein X is O and m and n are not both 1. . 5 In yet another aspect the invention provides a compound of the invention wherein X is

S(O), and m and n are both 1.

In a further aspect the invention provides a compound of the invention wherein X is

S(O)z,nis2 and mis 0.

In a still further aspect the invention provides a compound of the invention wherein X is S(O)h,nis0and mis 2.

In another aspect the invention provides a compound of the invention wherein X is O and m and n are both 1.

In a still further aspect the present invention provides a compound of formula (Ia):

R™

W

, (12) oi Dav

X ay wherein X is as defined above; Y is CH or N; R* is as defined for optional substituents on optionally substituted phenyl (above); and R!® is mono-substituted by C;.6 alkyl, C37 cycloalkyl, phenyl {optionally substituted by halo (for example fluoro), Ci4 alkyl (for example methyl), C;4 alkoxy (for example methoxy), CF; or OCF}, S(0)2(C,1.4 alkyl) (for example S(0),CH;, S(0)2CH,CHs or S(0),CH(CHs)z), S(0)2(Ci4 fluoroalkyl) (for example S(O),CF; or S(0),CH,CF3), S(O);phenyl {optionally substituted (such as mono-substituted) by halo (for example chloro), cyano, C4 alkyl, Ci. alkoxy, CFs, OCF3, S(O)(Ci-4 alkyl) (for example S(0),CHj; or S(0),CH,CH,CH3) or S(0)2(C1.4 fluoroalkyl) (for example

S(0),CH,CF3)}, benzyl {optionally substituted by halo (for example chloro or fluoro), C;4 alkyl, C4 alkoxy (for example methoxy), CF; or OCFs}, C(O)H, C(OXC,4 alkyl), benzoyl {optionally substituted by halo (for example chloro or fluoro), C4 alkyl (for example methyl), C;4 alkoxy, CFs or OCF3}, C(0)2(C14 alkyl), C(O)NH;, C(O)NH(C4 alkyl) or

C(O)NHphenyl {optionally substituted by halo (for example fluoro), C4 alkyl, C,4 alkoxy,

CF; or OCF3}. R'? can also be S(0);N(C.4 alkyl).

In another aspect the present invention provides a compound of formula (Ib): 1

R'\ :

NN

(J X R*

N

(Ib) wherein X, Y, R!® and R* are as defined above.

In yet another aspect the present invention provides a compound of formula (Ic):

R'

N . q oD (lc)

Y

‘Oa

X

R®

R*® wherein X, Y, R'* and R* are as defined above, and R®is hydrogen, one or two halogen atoms (for example selected from chlorine and fluorine) or CFs. In another aspect of the invention R* is hydrogen.

In a further aspect the present invention provides a compound of formula (Id): rR" © Id

HN (1d)

SO

S ay wherein R'* and R* are as defined above.

In a still further aspect the present invention provides a compound of formula (Te):

O

0=¢ 2 0}

R ‘Sa o wherein R* and R* are as defined above.

In another aspect the present invention provides a compound of formula (If):

Ria . TNT 55 . (vy N o © R (i)

R2 5 wherein Y, R'™ R* and R* are as defined above.

In yet another aspect the present invention provides a compound of formula (Ig):

R* 4 hd 7» ~ 4a

N N o © R wherein R' and R* are as defined above.

In a further aspect the present invention provides a compound of formula (Ih): i >S © 7» = R4a

N o ©

CJ (th) 2a

R wherein R* and R* are as defined above.

In a still further aspect the present invention provides a compound of formula i): . S

R%? wherein R', R?** and R* are as defined above.

In another aspect the present invention provides a compound of formula (Ij): \ pL 0=S

W, ! ‘Oa

S—\

I7A\

P R% oo R wherein R? and R* are as defined above.

In yet another aspect the present invention provides a compound of formula (Ik):

R! oO 5=0 rR (Ik) Ré wherein R, R?* and R* are as defined above.

In a further aspect the present invention provides a compound of formula (11): o

N \ a Reo 0 4

R® R wherein R}, R?* and R* are as defined above.

In a still further aspect the present invention provides a compound of formula (Im):

GJ

N a) wherein R? and R* are as defined above.

In another aspect the present invention provides a compound of formula (In):

AT

R N

" 5 rR wherein R!, R* and R* are as defined above.

In yet another aspect of the invention there is provided a compound of formula (Ia), (Ib), (Ic) or (If) wherein R® is S(0)2(C1. alkyl), S(0)2(Ci.4 haloalkyl), S(O)(pheny)),

S(0)2N(C,4 alkyl), or phenyl.

In yet another aspect of the invention there is provided a compound of formula (Ic), 1D, ah), (i), (@j), (Ik), (Im) or (In) wherein R* is hydrogen, one or two halo (such as one chloro, one fluoro, one chloro and one fluoro or two fluoro) or CFs. R? is, for example in the 2-, 3-, or 3- and 5- positions on the phenyl ring.

In another aspect of the invention there is provided a compound of formula (Ia), (Tb), (Ic), (Id), (a5), (Ig), (1h), li), (Ik), (Im) or (In) wherein R* is in the 4-position on the phenyl ring.

In a further aspect of the invention there is provided a compound of formula (Ia), (Ib), (Ic), (Id), (le), (If), (Ig), (Ih), (Ii), (Ik), (Im) or (In) wherein R* is one or more of halo, hydroxy, nitro, S(Cy.s alkyl), S(OXC. alkyl), S(0)2(Ci. alkyl), S(0)2NHz, S(0)-NH(Ci.6 alkyl), S(O)%N(C,. alkyl), cyano, C, alkyl, Ci alkoxy, CH,S(0)(C,.6 alkyl), OS(0)2(C1.6 alkyl), OCH;heteroaryl (such as OCHytetrazolyl), OCH.CO:H, OCH;COy(C.6 alkyl),

OCH,C(O)NH,, OCH,C(O)NH(C, ¢ alkyl), OCHCN, NH;, NH(C, 6 alkyl), N(C,.¢ alkyl),

C(O)NH,, C(O)NH(C,. alkyl), C(O)N(C1.s alkyl),, CO-H, CO2(Ch.6 alkyl), NHC(O)(C1.6 alkyl), NHC(0)O(C\.¢ alkyl), NHS(0)x(C1.6 alkyl), CF, CHF, CHoF, CH,CF;, OCF;, heteroaryl or heteroaryl(C,.4 alkyl); wherein the foregoing heteroaryl group (such as tetrazolyl) are optionally substituted by halo, hydroxy, nitro, S(Ci4 alkyl), S(0)(C,.4 alkyl),

S(0)y(C1.4 alkyl), S(0);NH,, S(0),NH(Ci.4 alkyl), S(0);N(C1.4 alkyl), cyano, C4 alkyl, C4 . 5 alkoxy, C(O)NH,, C(O)NH(C.4 alkyl), C(O)N(C,.4 alkyl)z, COzH, CO2(Ci4 alkyl),

NHC(0)(C;4 alkyl), NHS(0),(C14 alkyl), CF or OCF; {and in a further aspect of the invention the foregoing heteroaryl groups (such as tetrazolyl) are optionally substituted by C,. 4 alkyl}.

In a still further aspect of the invention there is provided a compound of formula (Ia), (Ib), (Ic), dd), Te), If), Ig), (Ih), (ID), (Tj), (Ik), (Im) or (In) wherein R™* is halogen (such as chloro or fluoro), cyano, C14 alkyl, Ci alkoxy, S(Ci4 alkyl), S(0)2(Ci4 alkyD), 0S(0)(C14 alkyl) or carboxamide.

The compounds listed in Tables I to XIV illustrate the invention.

~

Sia lee gle |x lle le ols a |F [Ns |e |v Is [= |= IQ ~ Il [Vi {\© Jv [VO Vn |\"n [© [|O z |4 vr |= g j@

X = |= | == |=

MERE) AEE 2 la 1.8 = 1a |e

Le [8 Bolas _ 21213121812 133 ‘SERERERERERERE re} CEERER EY SERRE

S Ss |g ls |S|8S [E85 |S

Lv lwo | lo |O |O JO JV 2 IEE |E|B|B EE

TOE ee _ lala oO Oo |0 [0 pad Sr? Naw” Nae” x > 72 @B |@A A a 2 J

So

Ss x g 2 = lglg l=ig|g = 2 |= ta gie le |gic 8 |= 2 |g =) sls l= 18 ls |S |0o = | 6 pe a |l&alae la |[&l8 [a KR 2 slg 12 15128 |5 a |= 5 28 |813 [883 8 [3 2 sis |5 (e518 |2i=|8 |8& & ERERERE RENEE RINERE 8 2 828818 |% |&|E|%

Ff] = (=% ja! & FI [SI FA A A I-A A A Al A 4

[3] [-— [=] 2 Zz re) - [+ = [o} 3 o oO NN jen | jn ~~

=I I Is |= Jens cn |oo ved nn I= 10 |N jen |< 0 [nn nn [vo [Wn vo ln wn DP RY =

IY g ~~ 5) = 3 = g 5 £ o o o Ele le lBlnl|E El, 2 s1SIBISIZIEIEIEIEIE| & = E1813 [55858 = <t <+ |< |= | |e |< j= |<

ARARIAiIAl2AARIAIR la lie ieigleigleie ele nA A lA |&# aR |F | F&A ~~ = Ss (1 a

Q < cr Zz 3 EEREREEE E =] glgl2lg|lg| gla |a = 2 2 l2l2lE EEE IEE 5 2 JET = = = = r= = A = 2

Fi AERCRERERERERE 2

[9] LQ |LO Jo|L JL LL |O a §1=15|8|819(8|81|59|§8 a

TIE |E|E IE (EE [2 [3 8

E|A|E|E|E|E |B |E|E|8 S a, oo wn ual Fo oli Ke s JN Fo sl fo ol Ha o 3 Ka o!

Olz OS |C |B |0 (8 (5 |S g — 2 < = — {ON [on IF ny ~ Joo oo al al Ra Ea Ee] — ]— ~ wv rN 2 E2277 |S = RCE Ea zg 2 |.2 == |S o|8 §|8 |o|o |B

HS [5 (8 |8

L233 : g 8 |& |& |S + |< [4 |< |.2 — + < Slama ~ O 0 [0 [OO |0

Vo) Nae? FS PN? Sa | ee

A »< nn Aa laa |# [7 = 2 14

XL

—_ | Co)

IE pad £ | = fo of fa oll Fu oll Fo ofl Fao = |= 2 glE| = g § | 8 ei . £ |S £

LO | 2 . |B |B . |2 < |= x 3 © ~ a 3 1S 5

ANN . z ~ @

CN

Ala Ss © = o |o = “ 5 |& E > Z |Z |0 0 |z

L

Ct “|Z |Z °

[22] — < g 8 uy | pm | pe— g HEE = 8 SHEERS 2 : £22 frm 8 = 5 [3 : RE ¥e 21288 2 |. £ =z |5 |E |B © |a = Ssi1g 18 |8 |S o 2 210 le lo °o pt ala |E |E |&

Z, — (3) =) 2 2 o 5 = = o = 5 2. =}

E a. 3S E o ~ 0 Z AN ln | (wn wy co |<t (a ERE

I SIRI [8 IR IA |B [© NEE F218 IR IQ |X [23 17s} SC vn |© | vn vn nn |\O O |\O O |O |v [vn |\O |n ’ = 3 IE

EE

=: I> = y— = - = IEEE |B > >= > S 5 5 & |& | |E 8 [Ea |e g 9g Q ERERERE 3S |3 |= = E g @ la a |a 2 >| |e o fo) VU jo jY | |g [© s lola leg m[>mleloiei2l8 le iS |S slg |>18 15 mS |S |21S|5|2 25 |8|2 (5 |= |S |s |S |S |s clelEl=slE|sl=(g|gl2|=(8|=2|818|2|8 (2% |8|&

SIE|E|E|E|EE|S |S (5 |B |S |S |E|8|5|5 (8 |8|E|S nl Boi BOE BoE Mr Eo OE TR Dh AA A RCO TREN UE A EU DRA NACI

Dl id ite | | |v || | |g |= |i | [= |F [FF | |=

Alalalalaialalalalalalajalalaia apap) clelglelelelciclecleleleleieii2igl2I2I2|2

Ald Flo a lBlAa |r |B |AaAlr |B warn nnn |ln

ULL e | | od | | | & @ jaa 8188 |8 sls |8 15 8 88 [8 wv (nn lnn |» vn vn |» wn |e |x jfile of fe oli fa oli aol fa ul zlzlzlzlzilz zz |2|Z2 |Z |Z2 |Z |Z |Z |0 |[©O |© © 0 |O — —f [omg] e—y = I= — g £88 gE |Eln z £55 £15] 1515 =] ERIERE =H [3 2 |& —l=l2i==I121212 === 1s13i1s F112 => 2lzle 2g jee |g|g(aie(elglela|g|E|(5|8|8

BEREAN AE RE RRR ER RERE-RE RENEE -RE REN fElEE EEE EEE Ele l8|E lela |e|s cRERERERERERE: FREE ERE- = = [3 |3 13 |3 [73 gEl2I2 (EEE |2 12a |RIE|E|a|El= lala |a | |& : slolsgjefglslglglg|ele(e(gle |g iz(B8l8 (88 |8

Elg|5lsig(s|8|5|18|58(5|8|8|8(8|E|8|8|8 |€ |§

SlEl2E€l2l2lealslElRI2IEIE|E|8 EEE (5 (8

Sls |B (BIEE|Elela|(s|E|E|eal€|E || |B (2. |8 2lgleEle|le|E|E|B|{E|E|8|E|E|E|B|D |E |E|E |E |E ~ — |e |e | et feet [et |e [oe _— NNN NN NN wow lon [~~ l= lO | | | (0 1 A nor a jo Ix Is 16 | |= IS = wn |v jo [© [vo (© [vo [vn |v | \O [] [] ge — | 0 >= CS EC IN ww |] R 8 § 8 [8 318 glE 7

Qo f= ==

E |= |8 |5 | 52 8(2 §

FRERERERE 8% 8 i= 2 wn | wn |rn |X [03 lei le|g|g|R|Z iE 28 = oc |EIEISIE|E[E|8||E 28 2 g |5 |S Ss |S |89 |sS (= = r=} 4S |% |B |L |% - |= | T oo |v ©

Siig in i222 |g |g IQ ET E lle |S |EIEIBIE(RIZ S&F 2 a Id j= ls |9 |= |< |= [j= =| =

Alalalalafalalajaia olo lo | |0o |0 |O |O IO |O ©)

D2 RB Real Ref Dna Rf DE Dd a rd Na

Ala la lal: |p jl; |a lz |» A = go o |e 8 (8 |e |g 2 13 le | 2 2 8 |2 |%

BS |B |B |B [I I]

Le CaS Ea bat mim EE le |e en [en TOPE uo 17%) wv |»

Tt [@] («3 N=] [+] ja nfl fo ol Ha ofl J of fo oli fo oli fu oJ fo ol Juul foo ao

SHICHICHRICHISHISHIP ICI I*NIe, ®) 2 = = zlEEEREIEIEE IB cIEIEIEIEIEIEIEIE|E |E 2 lg |g |= 2 a8 la (a = [=O = =O = = I TR = = R= = =)

ERERERERERERERERERE 2 : © ole [8 |g 8 (2 [8 (8 I

SIEIE|E|B|E|5|EIEIE |B

SESE 1E 18 EE (8 =!

OD | | |e |B |e |o |e |Q Q g|lE|E|E |S |E [EE |E |E g ~ joo On IO |— IN | | |nn |\© t~ nn

Slelzlz logs |= ~~ (© |r |e {00 [00 |e wv VO nn Jn nn tin [WO 170) p> <q 14 w—( | ow omg | meng | omy = |Z zElE 9 218 AER 2 2 5 |& EE |& o=w 3 | 3 3 [3 |3 . «wn | n nN |» |n 518 |=lo.|518 5 > | o = SEEIEIRIE fp : |B |E |B |S |E |E |E a < |< |< |= |< [+ |< = =

Q

J = ly - 8 5 x * 2 = 2 = ©» | wn 7p)

E

= ~ |= 2 = l= |z|E|8 ue! 5 REN EE Re

QO I= | | | aN] e OS [2 ]o |S |ai5 4 So |g |8 |2 [= 0 |o g Clo lol |e |& |b

Ss |S [S325 |& 8 oO |g [Ee |2

Q —- QO |= |= [|= |3 | 3 2 SEE [EE 2 |5 a NIE NIT a. S, NNN [en [en g AN [+ NAN |en on |=

Q

[3] : > — =) 2 Z r= o [-+ = g a.

E

=) &) AN en [= un ~~ wv

—_ +

Sielelzizlelzlzlz ig ls — |= © |= |= |= |S |© [© |& bl el eS DE Aad al ll A A = 2 or 0

A!

Oo=Ww ) : 2 vy | ped | pe Eo Ren Ba] = > len | > ES IE IES < = S15 18 5185 15 = |S |8 = [8 le a 18 |e aoe |e

Ss [3 |B 3 {3 |B 8 |B S18 |8 s |& y S18 |8 S18 | 8 80 ox [=] oc |e |S |S 5 |€ [5 5 |=

Ow 2 lo | |O 0 | jo > |e

Pi E IE |E Eg |E |8 = 18

Cl (i = g

Q — om TOIT > ha > 2 |B |BI|7 |B

So |e (22 [2 |2

PA 8 |8 |aa |e |= 2 3 19 |g |a |e |e 8 Sid (E18 |E |B [= -— = ole |B |= (3 2 2B lnm |E 5 IE IE IE |E 8 212818181912 |1212|3 ° = B= I IF I AC Ls Ws WL og 4] aN jen |B] |W IT on |n jen jon

[72] & g

QQ . 3] o > Zz © Le) = 5

[5] o = a. g 3) © Nl jen wn ~~

QO |< |= |S |= |n wv |= tS |vn jen |B DS VD jen f=

WO |[O WO (vv [WO | 0 {WO |w wn wo I~ Jo |v (VO INO I~ = — | — Q

AR

[D2 IV] 2 | 5 g|5 g : : |B £15

El 8 212 |= oS |g 7} Lal Ia BEN l= vn lvn |e > | 8 ~ g wv vn | 8 co |= | 4 |= Oo | >

ES B= <1 OO = = 18 12 |e |% LO 2 S 218(8 |S |& 22 88 2 [2 |§ =] 5 YS ERERERE S |B |& |& ec |2 2 [38 |X |S = 13 [8 |2 @ 1.0 glo =12 sls |B18 18 12 12121518 188 2 |. £1518 1% |Z |Z |E 2 le |x| |5 88

S 21g Ele BIZ SERENE gz |g EEE |=|%|s|8 (28 |8 5 8 E

ERE elslz|E|8le|E5le(eie |BIEIE|S|o|E pre, Q 0 |7T |n|~ |O 0

E | 8 SIEEIEIE IEE lz|E|IR|ISlE|E (822 == EEE EEIEERIEEEEREIEIEE ele lglelglg|lglg|lglglglg|lglglg|g({g|5is5 14 old |d lo {8d | © |] jojo 0 (© |i wij jv | |v clelalg lel leslie le le (ss 8s 8 |8 8 (5 [8 [18 |S a |aladldadalaalalen lava 80 | & ole lo|o loo |og|logie loo |lole le |e |e |e |g slgislslsi5i8l5|8|8|es|6|8|8ajg|sislels|c

S|Iaislsia|alals|s(sa|21si=2|=28|2|212]212 12 clelglelcjclalelele le |RSS EE (22 2 |8 . » pt y=] o p—t opt - youd - pu «pd vt gud oot o put -p— - pu . =] » put - o—t = p=

Tn { ¢ tv tv 18 1 1 1] 3 I EE A PL

Ce a aN Ee NE WE CeO Ee eS a a NE SO eS SS SS SS OS et NO at on len len len Jen len en Jen Jen Jen jon Jen Jen Jen Jon Jen [en {en Jen on - ICN [ON vy [No |b NO |— |N Jen | vn [VO [Bs |JO [ONO — |= |r Lan Ron I Bon -— aN IN NINN NINN NN fen wm vn +h = en fen es le= d= I~ |eN 00 |\© en [WO |= [vn [= |< |r woes nv vn ln [vn |VO |©O |©O wv Wn

AEHE

. £12122 & = = = EY 3 53 13 12 | 2 .

AA |» za lo BEDE) IS 3 > lo °

BER ERE-RE-RERE-BERE = 0 |e 3 (oie igijo |S iy

Sk BB |& |B |8 8899

Tw (a | | | | Te oO /, oO 54 & @] =] = = > 3 2 vy ; aol fo oJ fo oJ fo oJ f= PUAN fo oli fa oli) a sfiy fe 2 % 15) = & 2 oc 2IERIBEIEEIEIRIERIS slg lglg lg|lgligll lg |e

CREE REREREREREREREE

. Ss lejle=slslslE2l8 (5 |s rary > Slajalise | esis lg Es = SIZ I12 121212121812 13 po 218818 |8|8|8|8 88 3 EERE RERERERERER ERE g z SS |Slg Els |8i2 2 |= / © |2 | || |D |B [¥ |g |v a = gE (8 |E|E |E|E |B |E |E |8 © 5)

E g 2 : & 3 o 8

[72] St [ QL £2 7) 2 g offal |= J f= oR [<= Jo oO > OOo ju |Z |Z [00 = 2 Zz £2 o [54 = 5 5) a. g 5}

Oo N jen | |n ~~

] 2 yp = |= ©

E 2 o hia g o = x E a

Q p> 8

ST

B

<t © _ 5 eS =

LO =) oO =

Cd ~~ = P20 = > an) ] ] ra) 2 = rey C <3 = B a pra

Bz

Zz E = : Wi 5

Cc) 8 = < p— = = 2 22 E 5 x i) he w O=W0 [=] [=] /\ 3 [oy 4s o 5 = 5 2 &. 3 a. E £ 2 g ES) o

Q 8 8 5 a = [7] o

O Q 3] Oo 2 = 2 5 gi =) BE A 8 - <x o o + 13] = = > [= > ° 3 = ir us = 5 = g

E. 2,

E £

Q 5) © oO wy

~~ $ “21 hon = |@ = 3 ~~

S j=] [7s]

QQ k:

[3]

Sg 8 [%[8 & D < & = =) 3 g 2 g | ,O = 8 g 3 o | 8 het nl ~ =

S |S |g (8 = So <] 218 [>| 8 2 L

BEIEIS2] 3 2 =o EA EE & «x 2 2 S

Lr)

Q [o] Q [o] ot sg |8ls|8 & 3 3 |53|3 |3 22 tr y == Be = “

TRI IT pd uN a Le =

I len |en fen [en g i = 8

AD .g © = : : = ! E

So £ ot e

[7]

S 8 -B = 2) E o Ob

[3] : P< = © 2 Zz = og = 5 2 8 (| on wv oO — wv

] [7p] 2 |= ele

Oo wr wy t+ — ox > r D (®) R= = nZ hs ey 8 — pu— . > oes p Sled re) < 5 |g |.8 « = 8 |B =~ a 18 |'5 . > 1 > > a \O [=V3 iy =H 04 = Cv 3 (3 |3 = I a (a |= ¥ © 215 15 2 pa Nl aN Ig!

Eg on on on £

Gy [=] 3 : g = g g 2

S 2 3 oO a oS

E, & gE [=]

[3] > 2 2 < o [=] = 5 2. g

Q

@) aN jen wy

:

A lg lo |— |v |v lo | jo |g |= ln leo : Swi lav lag (R12 I18 | 18 IA

Bln |v lo |v |v [vn |v [© |v |v |n ey =

E 2 z = _ 218 2 22 2 S15 |o = EE Eg = le = 5 | 3 2 5 |S 2! |813| |E|g|2|8|® c g [5 Sls (slg |g |8|E < | © Oo |o © |Q |o

SI5lSs|S|E2EE |S! |S|5 :

SERIE BIBS IEEE

« (EE [EET |Bi<+ |< |EIS = [J rr |r dD IH i I IF 1? 19 15 3% + lcd | |F jen |v | [en | | |= [on

Oo R< \ E o= S 8 5)

Ld 7} ; £0 — < re) = = 5 pd =] cleo lolHF |lolole le ole lo [20 I~ NV. NN I~ IT ET Smt | bt slalegw|egleaiegleglegle |e] 3 |a2[(3lsd|213l3131813 (313 3 REECE ERIERICR EEE ER ERERICR EE © BIB IRBIZIBIBIBIRIE IRI? |® ry n(n [nv | [nn jn jn 4 nn fn - en |en jen jen len len Jon jen jE Jon [on (en

Zo = =

E =

SE rh PENI he he A Re Rr hel he ~Rl AR A

Co lel lglg lglglglg|agldg|s ry o |lo|l8|oleo |0|0 [clo ola lo on Sie lsle le ele la lela |g |S g J=0 B= 0 R= = CV = =O = = = = = > > g lz lalz|z|leleg|(g|g|®|g8|7

AER ERE ER ERR ERE ERE

2. a la Sle ijgls (& |g ls |= (= g eed ase | |B |e EEE o RERERERERERERERERERERFE. © SI18151818|8 181818 |8 8 |8

[72]

Q Sigg [(5|8|8 (2181/8 |§8|8 ’ ye Sls|12Blsis(Sl9|8 218 | |9 c. » lo |S ||| |g || |2 |e |e : EEG |E|E(B|BIE|E|R BE 8 « |< [Old |= |= |= |< |v |= |= |= 5d ° >) p4 = ° = = 5 =] £ [= &) ~N en wv ~~ e a nv [en [A [vn IK Jo [en [m=

Oo [=~ [vo [ve jrn Jn Jn [© —_

E 2 = |= =

IE mls <3 EF |3 = |= a |lgliglg 9 |= elelje|e |g = |= Ea -E-NE-NE

Ela |e|e|e]e]s 3 3 [31312 [38 (3 |a

Llilg|lal|al|ajanla (1 leis (gg |e |g YT

EAR RE RENE Bl 539 | (2 |B |B (3

BEIT |< |E|E[E|E |S = rt I Ta |r Fe |v by |W ~~ on jen len [= [+ |< | en 2 |=

O « 2 |= |B

Wx So | 2 |'E 7 = [gS |= o=F ; 212

SE len = : oe « 3 [3 = giglglaielg o =z £18 |5

Sle jg |? le ld |= |O go | oo {| do

S132 lol |3ig [|3 SEAR

SESE IEE |(2]|8 < |B |9

SIP IT 2 |S |8 8 IC o EN EES v |v | SEE ~ 225 “ie? elves iv = 14 on jn jen fen en [en | 2 len =, << = g w—y | emeq | pm ~~ S Rial is ound | vent | ov p— [hp 2222 < {¥ |=

S|E|15|5| [=| |§ 5 TE £2 ee a 8 | E CRERE:

SEEN EYES cE

EEE |E(2|B|E 3 R= RRR © |e |e lo 8 a. a l'a a = 2 ls ls |S (|9 [8 |= = i

Ee EERE EN N= ENE = g Eu

Ss |B (3288 53 S Oo 10 |O [2 I I OL’ I 7 | = = |» Sr IN [Nr v lo lo lo |g [ss [|S [0 a x 7 |; “ZEEE EEE Of $202

E-NE-RE-NE:- q |S = > |= = gl1g|8|28|B|EBIlE |g g z |Z |=

E8882 82 3 Z12|2 <+ |= [= | |< | (+ |S = AAS 2 ao) < = 5 2

As ~ g§ o @ =

Wn en 1 [eo | [~ wi |v vn jn jn wn

BX = ~ ~ ] EL] ~~ ° |. 2 |. +

S13 ED . gS 212] EEE & >

SIS] 15 (813 2 1 1 < |S < |S |=

FE [EE .

SEE ENE EYES

£1558 |Z |3 S 818 |2|8 EE E

Wy |= jen | [~~ |\O _ - : jo = E 2 3

E % : giggle ie 5

AEE ERERE 8 2 0 |E|E |S |g w fz |E

SE ITIRI?IT

S15 (9 iv jn ..

SS Jen em jen [en =) - = § g £ E rl hr =) [W) > Eg OTA 5

ITT ty (@)] 5]

LSU I | po) o 8 1.5 ° 5 sles (g|&iB|® g 21822 (22 2

NEN EICHCEEE =

NANR [aaa la s}

SlI2 182 | |o o = alas Iss |= a to)

BB |&a |? |7AA |A a S

Sis e882 ‘0 = . > == === [=9 °

Z |Z [a |&+ |[«+ |< & q

ZRiZgR (2 k » ° 2 Z 9 ae o = 5 a. gE oS << | ~~ O wn

~~ $

[79] . > oN on

N Oo (Va) wv = |= = 8g | § £ |S

Q Q

. , (EE x < |< g

[4] = [&] =~ 5 ] 5 £ |g 75] 2

Kal [<4 ) 0 : 2 |x =

Zz | go

JE wn

LS, = Sr [aa J X= = = =

E x pA & ~ |B a Call N= ° 51|@ [7%] -= Bs = =e) 2 27 & 22 & 5 |E o 3 |= w [72] » HRS 2 g | 8 a. gS (|S . g QQ QO

S g | 8 o <+ |<

Z

=) = Z [+] ge] = 5 2. g

Q

@) ol

In yet another aspect the invention provides each individual compound listed in the tables above.

The compounds of formula (I), (Ia), (Ib), (Ic), (1d), (Ie), (If), (Ig), (Ih), (i), (1j), (Ik), (11), (Im) and (In) are all compounds of the invention can be prepared as shown below.

A compound of the invention wherein R' is an N-linked optionally substituted heterocycle can be prepared by reacting a compound of formula (II): cl Rr

RENN A

(CH,),—X—(CH,),—R* (Il) wherein R% R3, R*, m, n, A and X are as defined above, with a compound R'H (wherein the H is on a heterocycle ring nitrogen atom) wherein R' is as defined above, in the presence of a suitable base (for example a tri(C,. alkyl)amine such as triethylamine or Hunig's base), in a suitable solvent (such as a chlorinated solvent, for example dichloromethane) and, for example, at a room temperature (for example 10-30°C), optionally in the presence of sodium iodide.

A compound of the invention, wherein R? is hydrogen, can be prepared by coupling a compound of formula (II):

HN I

(CH,),—X—(CH,) —R* (iN) wherein R*, m, n, A and X are as defined above, with a compound of formula (IV):

R" H

AN, w

R? 0 wherein R' and R? are as defined above, in the presence of NaBH(OAc); (wherein Ac is

C(O)CHa) in a suitable solvent (such as a chlorinated solvent, for example dichloromethane) at room temperature (for example 10-30°C).

A compound of the invention, wherein R? is hydrogen, can be prepared by coupling a compound of formula (III):

HN A

(CH,),—X—(CH,),—R* (Il) wherein R*, m, n, A and X are as defined above, with a compound of formula (V):

Rr’

PON

R® L W) wherein R' and R? are as defined above and L is a leaving group such as halogen, tosylate, mesylate or triflate, in the presence of a base, such as potassium carbonate, in a suitable solvent (such as dioxane, acetonitrile or isopropanol) at temperatures from 60°C up to the boiling point of the solvent.

Alternatively, compounds of the invention can be prepared according to Schemes 1-7 (below).

Alternatively, compounds of the invention can be prepared by using or adapting methods described in WO01/87839, EP-A1-1013276, WO00/08013, W099/38514, 'WO99/04794;, WO00/76511, WO00/76512, WO00/76513, WO00/765 14, WO00/76972 or US 2002/0094989.

The starting materials for these processes are either commercially available or can be prepared by literature methods, adapting literature methods or by following or adapting

Methods herein described.

In a still further aspect the invention provides processes for preparing the compounds of formula (I), (Ia), (Ib), (Ic), (Id), (Ie), (If), (Ig), (Th), (Ii), (1j), (Ik), (11), (Im) and (In). Many of the intermediates in the processes are novel and these are provided as further features of the invention. 3

The compounds of the invention have activity as pharmaceuticals, in particular as modulators (such as agonists, partial agonists, inverse agonists or antagonists) of chemokine receptor (such as CCRS) activity, and may be used in the treatment of autoimmune, inflammatory, proliferative or hyperproliferative diseases, or immunologically-mediated diseases (including rejection of transplanted organs or tissues and Acquired

Immunodeficiency Syndrome (AIDS)).

The compounds of the present invention are also of value in inhibiting the entry of viruses (such as human immunodeficiency virus (HIV)) into target calls and, therefore, are of value in the prevention of infection by viruses (such as HIV), the treatment of infection by viruses (such as HIV) and the prevention and/or treatment of acquired immune deficiency syndrome (AIDS).

According to a further feature of the invention there is provided a compound of the formula (I), (la), (Ib), (Ic), (Id), (Ie), (If), (Ig), (Th), (Ii), (Tj), (Xk), (11), (Im) or (In) (for example a compound of formula (I), (Ia), (Ib), (Ic), (Id) or (Ie)), or a pharmaceutically acceptable salt thereof or a solvate thereof, for use in a method of treatment of a warm blooded animal (such as man) by therapy (including prophylaxis).

According to a further feature of the present invention there is provided a method for : 5 modulating chemokine receptor activity (such as CCR5 receptor activity) in a warm blooded animal, such as man, in need of such treatment, which comprises administering to said animal an effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof or a solvate thereof.

The present invention also provides the use of a compound of the formula (I), (Ia), (), (Ic), (1d), (le), (If), (Ag), (Ih), (Ii), (Tj), (TK), (11), (Im) or (In) (for example a compound of formula (I), (a), (Ib), (Ic), (Id) or (Ie), or a pharmaceutically acceptable salt thereof or a solvate thereof, as a medicament, such as a medicament for the treatment of transplant rejection, respiratory disease, psoriasis or rheumatoid arthritis (such as rheumatoid arthritis). [Respiratory disease is, for example, COPD, asthma {such as bronchial, allergic, intrinsic, extrinsic or dust asthma, particularly chronic or inveterate asthma (for example late asthma or airways hyper-responsiveness)} or rhinitis {acute, allergic, atrophic rhinitis or chronic rhinitis including rhinitis caseosa, hypertrophic rhinitis, rhinitis purulenta, rhinitis sicca or rhinitis medicamentosa; membranous rhinitis including croupous, fibrinous or pseudomembranous rhinitis or scrofoulous rhinitis; seasonal rhinitis including rhinitis nervosa (hay fever) or vasomotor rhinitis}; and is particularly asthma or rhinitis].

In another aspect the present invention provides the use of a compound of the formula (D), (1a), (Ib), (Ic), Ad), (e), (If), (Ig), (Th), (1), (Ij), (Ik), (1), (im) or (In) (for example a compound of formula (I), (Ia), (Ib), (Ic), (Id) or (Ie)), or a pharmaceutically acceptable salt thereof or a solvate thereof, in the manufacture of a medicament for use in therapy (for example modulating chemokine receptor activity (such as CCRS receptor activity (such as rheumatoid arthritis)) in a warm blooded animal, such as man).

The invention also provides a compound of the formula (I), (Ia), (Ib), (Ic), (1d), (Te), (af), (Ig), (Th), (Ii), (Ij), (Ik), (11), (Im) or (In) (for example a compound of formula D, (1a), (Ib), (Ic), (Id) or (Ie)), or a pharmaceutically acceptable salt thereof or a solvate thereof, for : 30 use as a medicament, such as a medicament for the treatment of rheumatoid arthritis.

In another aspect the present invention provides the use of a compound of the formula (D), (Ta), (Ib), (Ic), (Id), (Ie), (AD), (Ag), (Th), (1), (1), (Ik), (11), (Im) or (In) (for example a compound of formula (I), (a), (b), (Ic), (Id) or (Ie)), or a pharmaceutically acceptable salt thereof or a solvate thereof, in the manufacture of a medicament for use in therapy (for example modulating chemokine receptor activity (such as CCRS receptor activity (such as rheumatoid arthritis)) in a warm blooded animal, such as man).

The invention further provides the use of a compound of formula (I), (Ia), (Ib), (Ic), (1d), (le), (If), (Ig), (Th), (Ii), (Ij), (Ik), (11), (Im) or (In) (for example a compound of formula (), (12), (Ib), (Ic), (Id) or (Ie)), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of: (1) (the respiratory tract) obstructive diseases of airways including: chronic obstructive pulmonary disease (COPD) (such as irreversible COPD); asthma {such as bronchial, allergic, intrinsic, extrinsic or dust asthma, particularly chronic or inveterate asthma (for example late asthma or airways hyper-responsiveness)}; bronchitis {such as eosinophilic bronchitis} ; acute, allergic, atrophic rhinitis or chronic rhinitis including rhinitis caseosa, hypertrophic rhinitis, rhinitis purulenta, rhinitis sicca or rhinitis medicamentosa; membranous rhinitis including croupous, fibrinous or pseudomembranous rhinitis or scrofoulous rhinitis; seasonal rhinitis including rhinitis nervosa (hay fever) or vasomotor rhinitis; sarcoidosis; farmer’s lung and related diseases; nasal polyposis; fibroid lung or idiopathic interstitial pneumonia; (2) (bone and joints) arthrides including rheumatic, infectious, autoimmune, seronegative : spondyloarthropathies (such as ankylosing spondylitis, psoriatic arthritis or Reiter’s disease), Behget’s disease, Sjogren’s syndrome or systemic sclerosis; (3) (skin and eyes) psoriasis, atopic dermatitis, contact dermatitis or other eczmatous dermitides, seborrhoetic dermatitis, Lichen planus, Phemphigus, bullous Phemphigus,

Epidermolysis bullosa, urticaria, angiodermas, vasculitides erythemas, cutaneous eosinophilias, uveitis, Alopecia areata or vernal conjunctivitis; (4) (gastrointestinal tract) Coeliac disease, proctitis, eosinophilic gastro-enteritis, mastocytosis, Crohn’s disease, ulcerative colitis, irritable bowel disease or food-related allergies which have effects remote from the gut (for example migraine, rhinitis or eczema); (5) (Allograft rejection) acute and chronic following, for example, transplantation of kidney, . 30 heart, liver, lung, bone marrow, skin or cornea; or chronic graft versus host disease; and/or (6) (other tissues or diseases) Alzheimer’s disease, multiple sclerosis, atherosclerosis,

Acquired Immunodeficiency Syndrome (AIDS), Lupus disorders (such as lupus erythematosus or systemic lupus), erythematosus, Hashimoto’s thyroiditis, myasthenia gravis, type I diabetes, nephrotic syndrome, eosinophilia fascitis, hyper IgE syndrome, leprosy (such as lepromatous leprosy), Peridontal disease, Sezary syndrome, idiopathic thrombocytopenia pupura or disorders of the menstrual cycle; in a warm blooded animal, such as man.

The present invention further provides a method of treating a chemokine mediated disease state (such as a CCR5 mediated disease state) in 2a warm blooded animal, such as man, which comprises administering to a mammal in need of such treatment an effective amount of a compound of formula (I), (1a), (Ib), (Ic), (1d), (le), (If), (Ig), (Ih), (Ii), (Ij), (kk), (11), (Im) or (In) (for example a compound of formula (I), (1a), (Ib), (Ic), (1d) or (le), or a pharmaceutically acceptable salt thereof or solvate thereof.

In order to use a compound of the invention, or a pharmaceutically acceptable salt thereof or solvate thereof, for the therapeutic treatment of a warm blooded animal, such as man, in particular modulating chemokine receptor (for example CCRS receptor) activity, said ingredient is normally formulated in accordance with standard pharmaceutical practice as a pharmaceutical composition.

Therefore in another aspect the present invention provides a pharmaceutical composition which comprises a compound of the formula (I), (Ia), (Ib), (Ic), (1d), (Te), (If), (Ig), (Th), (Ii), (Ij), (Ik), (11), (Im) or (In) (for example a compound of formula (I), (Ia), (Ib), (Ic), (1d) or (Ie)), or a pharmaceutically acceptable salt thereof or a solvate thereof (active ingredient), and a pharmaceutically acceptable adjuvant, diluent or carrier. In a further aspect the present invention provides a process for the preparation of said composition which comprises mixing active ingredient with a pharmaceutically acceptable adjuvant, diluent or carrier. Depending on the mode of administration, the pharmaceutical composition will, for example, comprise from 0.05 to 99 %w (per cent by weight), such as from 0.05 to 80 %w, for example from 0.10 to 70 %w, such as from 0.10 to 50 %w, of active ingredient, all percentages by weight being based on total composition.

The pharmaceutical compositions of this invention may be administered in standard manner for the disease condition that it is desired to treat, for example by topical (such as to : 30 the lung and/or airways or to the skin), oral, rectal or parenteral administration. For these purposes the compounds of this invention may be formulated by means known in the art into the form of, for example, aerosols, dry powder formulations, tablets, capsules, syrups, powders, granules, aqueous or oily solutions or suspensions, (lipid) emulsions, dispersible powders, suppositories, ointments, creams, drops and sterile injectable aqueous or oily solutions or suspensions.

A suitable pharmaceutical composition of this invention is one suitable for oral administration in unit dosage form, for example a tablet or capsule which contains between 0.lmg and 1g of active ingredient.

In another aspect a pharmaceutical composition of the invention is one suitable for intravenous, subcutaneous or intramuscular injection.

Each patient may receive, for example, an intravenous, subcutaneous or intramuscular dose of 0.01mgkg™ to 100mgkg™! of the compound, for example in the range of 0.1mgkg™ to 20mgkg of this invention, the composition being administered 1 to 4 times per day. The intravenous, subcutaneous and intramuscular dose may be given by means of a bolus injection. Alternatively the intravenous dose may be given by continuous infusion over a period of time. Alternatively each patient will receive a daily oral dose which is approximately equivalent to the daily parenteral dose, the composition being administered 1 to 4 times per day.

The following illustrate representative pharmaceutical dosage forms containing the compound of formula (I), (1a), (Ib), (Ic), (Id), (Ie), (If), (Ig), (Ih), Ti), (Ij), (Ik), (11), (Im) or (In) (for example a compound of formula (I), (Ia), (Ib), (Ic), (Id) or (Ie)),ora pharmaceutically acceptable salt thereof or a solvent thereof (hereafter Compound X), for therapeutic or prophylactic use in humans:

(a)

EL

(b)

I LI

(co) d

(e)

CE

Buffers, pharmaceutically-acceptable cosolvents such as polyethylene glycol, polypropylene glycol, glycerol or ethanol or complexing agents such as hydroxy-propyl B- cyclodextrin may be used to aid formulation.

The above formulations may be obtained by conventional procedures well known in the pharmaceutical art. The tablets (a)-(c) may be enteric coated by conventional means, for example to provide a coating of cellulose acetate phthalate.

The invention further relates to combination therapies or compositions wherein a compound of formula (I), or a pharmaceutically acceptable salt, solvate or a solvate of a salt thereof, or a pharmaceutical composition comprising a compound of formula (I), ora pharmaceutically acceptable salt, solvate or a solvate of a salt thereof, is administered concurrently (possibly in the same composition) or sequentially with an agent for the treatment of any one of the above disease states.

In particular, for the treatment of the inflammatory diseases rheumatoid arthritis, psoriasis, inflammatory bowel disease, COPD, asthma and allergic rhinitis a compound of the invention can be combined with a TNF-a inhibitor (such as an anti-TNF monoclonal antibody (such as Remicade, CDP-870 and D.sub2.E.sub7.), or a TNF receptor immunoglobulin molecule (such as Enbrel.reg.)), a non-selective COX-1/ COX-2 inhibitor (such as piroxicam or diclofenac; a propionic acid such as naproxen, flubiprofen, fenoprofen, ketoprofen or ibuprofen; a fenamate such as mefenamic acid, indomethacin, sulindac or apazone; a pyrazolone such as phenylbutazone; or a salicylate such as aspirin), a COX-2 inhibitor (such as meloxicam, celecoxib, rofecoxib, valdecoxib or etoricoxib) low dose methotrexate, lefunomide; ciclesonide; hydroxychloroquine, d-penicillamine or auranofin, or parenteral or : 25 oral gold.

The present invention still further relates to the combination of a compound of the invention together with: ¢ a leukotriene biosynthesis inhibitor, a 5-lipoxygenase (5-LO) inhibitor or a 5- lipoxygenase activating protein (FLAP) antagonist, such as zileuton, ABT-761, fenleuton, tepoxalin, Abbott-79175, Abbott-85761, an N-(5-substituted)-thiophene-2-

alkylsulfonamide, a 2,6-di-tert-butylphenol hydrazones, a methoxytetrahydropyran such as Zeneca ZD-2138, SB-210661, a pyridinyl-substituted 2-cyanonaphthalene compound such as L-739,010; a 2-cyanoquinoline compound such as L-746,530; an indole or quinoline compound such as MK-591, MK-886 or BAY x 1005; oo 5 ¢ a receptor antagonist for a leukotriene LTB.sub4., LTC.sub4., LTD.sub4. or

LTE.sub4. selected from the group consisting of a phenothiazin-3-one such as L- 651,392; an amidino compound such as CGS-25019c; a benzoxalamine such as ontazolast; a benzenecarboximidamide such as BIIL 284/260; or a compound such as zafirlukast, ablukast, montelukast, pranlukast, verlukast (MK-679), RG-12525, Ro- 245913, iralukast (CGP 45715A) or BAY x 7195; o a PDE4 inhibitor including an inhibitor of the isoform PDE4D; e an antihistaminic H.subl. receptor antagonist such as cetirizine, loratadine, desloratadine, fexofenadine, astemizole, azelastine or chlorpheniramine; e a gastroprotective H.sub2. receptor antagonist; e an a.subl.- and a.sub2.-adrenoceptor agonist vasoconstrictor sympathomimetic agent, such as propylhexedrine, phenylephrine, phenylpropanolamine, pseudoephedrine, naphazoline hydrochloride, oxymetazoline hydrochloride, tetrahydrozoline hydrochloride, xylometazoline hydrochloride or ethylnorepinephrine hydrochloride; ¢ an anticholinergic agent such as ipratropium bromide, tiotropium bromide, oxitropium bromide, pirenzepine or telenzepine; e a f.subl.- to B.sub4.-adrenoceptor agonist such as metaproterenol, isoproterenol, isoprenaline, albuterol, salbutamol, formoterol, salmeterol, terbutaline, orciprenaline, bitolterol mesylate or pirbuterol, or a methylxanthanine including theophylline and aminophylline; sodium cromoglycate; or a muscarinic receptor (M1, M2, and M3) antagonist; e an insulin-like growth factor type I (IGF-1) mimetic; « an inhaled glucocorticoid with reduced systemic side effects, such as prednisone, , prednisolone, flunisolide, triamcinolone acetonide, beclomethasone dipropionate, budesonide, fluticasone propionate or mometasone furoate; e an inhibitor of a matrix metalloprotease (MMP), such as a stromelysin, a collagenase, or a gelatinase or aggrecanase; such as collagenase-1 (MMP-1), collagenase-2 (MMP- 8), collagenase-3 (MMP-13), stromelysin-1 (MMP-3), stromelysin-2 (MMP-10), and stromelysin-3 (MMP-11) or MMP-12;

e a modulator of chemokine receptor function such as CCR1, CCR2, CCR2A, CCR2B,

CCR3, CCR4, CCRS, CCR6, CCR7, CCR8, CCR9, CCR10 and CCR11 (for the C-C family); CXCR1, CXCR2, CXCR3, CXCR4 and CXCRS (for the C-X-C family) and

CX3CR1 for the C-X3-C family; e an osteoporosis agent such as roloxifene, droloxifene, lasofoxifene or fosomax; ¢ an immunosuppressant agent such as FK-506, rapamycin, cyclosporine, azathioprine or methotrexate; e a compound useful in the treatment of AIDS and/or HIV infection for example: an agent which prevents or inhibits the viral protein gp120 from engaging host cell CD4 {such as soluble CD4 (recombinant); an anti-CD4 antibody (or modified / recombinant antibody) for example PRO542; an anti-group120 antibody (or modified / recombinant antibody); or another agent which interferes with the binding of group120 to CD4 for example BMS806}; an agent which prevents binding to a chemokine receptor, other than CCRS5, used by the HIV virus {such as a CXCR4 agonist or antagonist or an anti-CXCR4 antibody}; a compound which interferes in the fusion between the HIV viral envelope and a cell membrane {such as an anti- group 41 antibody; enfuvirtide (T-20) or T-1249}; an inhibitor of DC-SIGN (also known as CD209) {such as an anti-DC-SIGN antibody or an inhibitor of DC-SIGN binding}; a nucleoside/nucleotide analogue reverse transciptase inhibitor {for example zidovudine (AZT), nevirapine, didanosine (ddI), zalcitabine (ddC), stavudine (d4T), lamivudine (3TC), abacavir, adefovir or tenofovir (for example as free base or as disoproxil fumarate)}; a non-nucleoside reverse transciptase inhibitor {for example nevirapine, delavirdine or efavirenz}; a protease inhibitor {for example ritonavir, indinavir, saquinavir (for example as free base or as mesylate salt), nelfinavir (for example as free base or as mesylate salt), amprenavir, lopinavir or atazanavir (for example as free base or as sulphate salt)}; a ribonucleotide reductase inhinbitor {for example hydroxyurea}; or an antiretroviral {for example emtricitabine}; or, e an existing therapeutic agent for the treatment of osteoarthritis, for example a non- steroidal anti-inflammatory agent (hereinafter NSAID's) such as piroxicam or diclofenac, a propionic acid such as naproxen, flubiprofen, fenoprofen, ketoprofen or ibuprofen, a fenamate such as mefenamic acid, indomethacin, sulindac or apazone, a pyrazolone such as phenylbutazone, a salicylate such as aspirin, a COX-2 inhibitor such as celecoxib, valdecoxib, rofecoxib or etoricoxib, an analgesic or intra-articular therapy such as a corticosteroid or a hyaluronic acid such as hyalgan or synvisc, or a

P2X7 receptor antagonist.

The present invention still further relates to the combination of a compound of the invention together with: (i) a tryptase inhibitor; (ii) a platelet activating factor (PAF) : 5 antagonist; (iii) an interleukin converting enzyme (ICE) inhibitor; (iv) an IMPDH inhibitor; (v) an adhesion molecule inhibitor including a VLA-4 antagonist; (vi) a cathepsin; (vii) a

MAP kinase inhibitor; (viii) a glucose-6 phosphate dehydrogenase inhibitor; (ix) a kinin-

B.subl. - and B.sub2. -receptor antagonist; (X) an anti-gout agent, €.g., colchicine; (xi) a xanthine oxidase inhibitor, e.g., allopurinol; (xii) an uricosuric agent, €.g., probenecid, sulfinpyrazone or benzbromarone; (xiii) a growth hormone secretagogue; (xiv) a transforming growth factor (TGF); (xv) a platelet-derived growth factor (PDGF); (xvi) a fibroblast growth factor, e.g., basic fibroblast growth factor (bFGF); (xvii) a granulocyte macrophage colony stimulating factor (GM-CSF); (xviii) a capsaicin cream; (xix) a Tachykinin NK.subl. and

NK _sub3. receptor antagonist selected from the group consisting of NKP-608C; SB-233412 (talnetant); and D-4418; (xx) an elastase inhibitors selected from the group consisting of UT- 77 and ZD-0892; (xxi) a TNFa converting enzyme inhibitor (TACE); (xxii) an induced nitric oxide synthase inhibitor (iNOS); or (xxiii) a chemoattractant receptor-homologous molecule expressed on TH2 cells (a CRTH2 antagonist).

The invention will now be illustrated by the following non-limiting Examples in which, unless stated otherwise: (i) temperatures are given in degrees Celsius (°C); operations were carried out at room or ambient temperature, that is, at a temperature in the range of 18-25°C; (ii) organic solutions were dried over anhydrous magnesium sulfate; evaporation of solvent was carried out using a rotary evaporator under reduced pressure (600-4000 Pascals; 4.5-30 mm Hg) with a bath temperature of up to 60°C; (iii) chromatography unless otherwise stated means flash chromatography on silica gel; thin layer chromatography (TLC) was carried out on silica gel plates; where a “Bond Elut” column is referred to, this means a column containing 10g or 20g of silica of 40 micron particle size, the silica being contained in a 60m] disposable syringe and supported by a porous disc, obtained from Varian, Harbor City, California, USA under the name “Mega

Bond Elut SI”. Where an "Isolute™ SCX column" is referred to, this means a column containing benzenesulphonic acid (non-endcapped) obtained from International Sorbent

Technology Ltd., 1st House, Duffryn Industial Estate, Ystrad Mynach, Hengoed, Mid

Glamorgan, UK. Where "Argonaut™ PS-fris-amine scavenger resin" is referred to, this means a tris-(2-aminoethyl)amine polystyrene resin obtained from Argonaut Technologies

Inc., 887 Industrial Road, Suite G, San Carlos, California, USA. (iv) in general, the course of reactions was followed by TLC and reaction times are given for . 5 illustration only; (v) yields, when given, are for illustration only and are not necessarily those which can be obtained by diligent process development; preparations were repeated if more material was required; (vi) when given, 'H NMR data is quoted and is in the form of delta values for major diagnostic protons, given in parts per million (ppm) relative to tetramethylsilane (TMS) as an internal standard, determined at 300 MHz using perdeuterio DMSO (CD;SOCD;) as the solvent unless otherwise stated; coupling constants (J) are given in Hz; (vii) chemical symbols have their usual meanings; SI units and symbols are used; (viii) solvent ratios are given in percentage by volume; (ix) mass spectra (MS) were run with an electron energy of 70 electron volts in the chemical ionisation (APCI) mode using a direct exposure probe; where indicated ionisation was effected by electrospray (ES); where values for m/z are given, generally only ions which indicate the parent mass are reported, and unless otherwise stated the mass ion quoted is the positive mass ion - (M+H); : (x) LCMS characterisation was performed using a pair of Gilson 306 pumps with Gilson 233

XL sampler and Waters ZMD4000 mass spectrometer. The LC comprised water symmetry 4.6x50 column C18 with 5 micron particle size. The eluents were: A, water with 0.05% formic acid and B, acetonitrile with 0.05% formic acid. The eluent gradient went from 95%

A t0 95% B in 6 minutes. Where indicated ionisation was effected by electrospray (ES); where values for m/z are given, generally only ions which indicate the parent mass are reported, and unless otherwise stated the mass ion quoted is the positive mass ion - (M+H)"; (xi) PS-NCO resin is an isocyanate resin and is available from Argonaut; (xii) Powder X-Ray Diffractometry (PXRD) analyses were performed using a Siemens

D5000. The X-ray powder diffraction spectra were determined by mounting a sample of the crystalline salt on Siemens single silicon crystal (SSC) wafer mounts and spreading out the sample into a thin layer with the aid of a microscope slide. The sample was spun at 30 revolutions per minute (to improve counting statistics) and irradiated with X-rays generated by a copper long-fine focus tube operated at 40kV and 40mA with a wavelength of 1.5406 angstroms. The collimated X-ray source was passed through an automatic variable divergence slit set at V20 and the reflected radiation directed through a 2mm antiscatter slit and a 0.2mm detector slit. The sample was exposed for 1 second per 0.02 degree 2-theta increment (continuous scan mode) over the range 2 degrees to 40 degrees 2-theta in theta- . 5 thetamode. The running time was 31 minutes and 41 seconds. The instrument was equipped with a scintillation counter as detector. Control and data capture was by means of a Dell

Optiplex 686 NT 4.0 Workstation operating with Diffract+ software. Persons skilled in the art of X-ray powder diffraction will realise that the relative intensity of peaks can be affected by, for example, grains above 30 microns in size and non-unitary aspect ratios which may affect analysis of samples. The skilled person will also realise that the position of reflections can be affected by the precise height at which the sample sits in the diffractometer and the zero calibration of the diffractometer. The surface planarity of the sample may also have a small effect.; and, (xiii) the following abbreviations are used:

THF tetrahydrofuran;

Boc tert-butoxycarbonyl

DMF N,N-dimethylformamide

DCM dichloromethane

DIPEA N,N-Diisopropylethylamine

R-BINAP R 2,2'-Bis(diphenylphosphino)-1,1’-binaphthyl

HATU O-(7-Azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate

EDCI ethyl dimethylaminopropyl carbodiimide

HOBT 1-hydroxybenzotriazole

Example 1

This Example illustrates the preparation of N-(3-phenyi-3-[4- methanesulphonylpiperazin- 1-yl}propyl)-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]- piperidine (Compound No. 8, Table I).

Vd 0=§, 3

N LP

3S aS

AN

N-(3-Phenyl-3-chloropropyl)-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]- piperidine (prepared according to Method D; 180mg) was added to a solution of N- methanesulphonylpiperazine (61mg) and triethylamine (0.102ml) in dichloromethane (10m) and the mixture was allowed to stand at room temperature for 16 hours. The reaction mixture was poured onto a 20g silica Bond Elut eluted with a solvent gradient (ethyl acetate - 25% methanol/ethyl acetate). The title compound was obtained, yield 67mg, MH' 612.

NMR (CDCl): 1.6-1.8 (m, 7H), 2.2-2.6(m, 9H), 2.7(m, 1H), 2.75 (s, 3H), 3.2 (m, 11H), 3.45 (m, 1H), 7.2 (d, 2H), 7.3 (m, 3H), 8.2 (m, 4H).

Example 2

This Example illustrates the preparation of N-(3-phenyl-3-[1-methanesulphonyl- piperidin-4-yljpropyl)-4-[2-(4-fluorophenylsulphony!)ethyl]piperidine (Compound No. 10,

Table I). 4

O=S, om, $ a 2

F

Sodium triacetoxyborohydride (267 mg) was added to a mixture of 3-(1- methanesulphonylpiperidin-4-y1)-3-phenylpropionaldehyde (247 mg) and 4-(2-[4- fluorophenylsulphonyljethyl)piperidine hydrochloride salt (288 mg) (CAS 313994-09-1) in dichloromethane (20 ml) and the mixture was stirred for 16 hours. The reaction mixture was washed successively with 2M sodium hydroxide (10 ml), water (10 ml) and brine (10 ml) and was dried. The residue obtained on removal of the solvent was chromatographed on a 20g silica Bond Elut column eluting with a solvent gradient (ethyl acetate - 20% methanol/ethyl acetate) to give the title compound, yield 250mg, MH" 551. . 5 NMR (CDCl): 1.2 (m, 5H), 1.4 (m, 4H), 1.6-1.8 (m, 8H), 2.0 (m, 3H), 2.4 (m, 1H), 2.5-2.6 (m, 2H), 2.8 (s, 3H), 2.85 (m, 2H), 3.1 (m, 2H), 3.7 (d, 1 H), 3.85 (d, 1H), 7.1 (m, 2H), 7.3 (m, SH), 7.9 (m, 2H).

Example 3

This Example illustrates the preparation of (S) N-(3-phenyl-3-[4-chlorobenzoyl- amino]propyl-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]piperidine (Compound No. 2,

Table IV). a vy

HN 0 52 [o} 4-Chlorobenzoyl chloride (76pl) was added to a solution of (S) N-(3-amino-3- phenylpropyl)4-[2-(4-methanesulphonylphenylsulphonyl)ethyl piperidine (280mg) and triethylamine (157ul) in dichloromethane (15ml) and the mixture was stirred for 1 hour then washed with water (15ml) and brine (15ml) and dried. Removal of the solvent gave the title compound as a white solid, yield 320mg, MH" 602.

NMR (d6 DMSO): 1.0 (m, 2H), 1.2 (m, 1H), 1.5 (m, 2H), 1.6 (m, 2H), 1.8 (m, 2H), 1.9 (m, 2H), 2.25 (m, 2H), 2.8 (m, 2H), 3.3 (m,3H), 3.4 m, 2H), 5.0 (q, 1H), 7.2 (m, 1H), 7.3 (m,3H), 7.5 (d, 2H), 7.85 (d, 2H), 8.2 (m, 4H), 8.9 (d, 1H).

Example 4 ’ This Example illustrates the preparation of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl}-4-(2- {[4-(methylsulfonyl)phenyl]sulfonyl} ethyl)piperidine (Compound No. 7, Table V).

o, 0 0, 0

Pe rai 0’ 0

PON

(R)-3-(3,5-Difluorophenyl)-3-(4-methanesulfonylphenyl)propionaldehyde (0.357 g, 1.1 mmol; Method E) was dissolved in dichloromethane (3 ml) at room temperature and 4-[2- (4-methanesulphonylphenyl-sulphonyl)ethyl]piperidine hydrochloride (0.368 g, 1 mmol;

Method B) was added as a single portion. After stirring for 0.5 h, sodium triacetoxyborohydride (0.211 g, 1 mmol) was added as a single portion and the reaction stirred for a further 1h. The mixture was then washed with saturated aqueous sodium hydrogen carbonate, the organics were separated and poured directly onto an SCX column.

Eluting with methanol followed by 20% 7M ammonia in methanol gave the product (0.319 g, 50%) as a white solid.

NMR: (d6-DMSO): 1.05 (m, 2H), 1.15 (m, 1H), 1.6 (m, 4H), 1.8 (br t, 2H), 2.2 (m, 2H), 2.3 (m, 2H), 2.8 (br d, 2H), 3.4 (m, 6H), 3.5 (m, 2H), 4.3 (br t, 1H), 7.1 (br t, 1H), 7.2 (d, 2H), 7.7 (d, 2H), 7.9 (d, 2H), 8.3 (m, 4H).

LCMS: 640.2 (MH).

Example 5

This Example illustrates the preparation of (R or S) N-(3-[4- methanesulphonylpiperazinyl}-3-phenylpropyl)-4-[2-(4-fluorophenyisulphonyl)ethyl]- piperidine (Compound 14, Table III). 054-0

N

(J sag oUNs &o

A solution of (R or S) N-(3-chloro-3-phenylpropyl)-4-[2-(4-fluorophenylsulphonyl)- ethyl]-piperidine (Method F; 310 mg) in dichloromethane (6 ml) was added to N- methanesulphonyl-piperazine hydrochloride (150 mg) followed by triethylamine (313 pl).

The mixture was stirred for 48 hours, diluted with dichloromethane (5 ml) and MP-carbonate -

resin (1.34g), PS-isocyanate resin (682 mg) and PS-thiophenol resin (577 mg) were added.

The mixture was stirred for 5 hours, filtered and the resins were washed with 10% methanol in dichloromethane (2x25 ml). The combined filtrates were evaporated to dryness and the residue was passed through a 20g Isolute column eluted with a solvent gradient of ethyl : 5 acetate-10% methanol/ethyl acetate to give the title compound, yield 81 mg; MH" 552.

NMR (CDCl): 1.12-1.32 (m, 4H), 1.52-1.66 (m, 4H), 1.76-1.93 (m, 3H), 2.08 (m, 1H), 2.21 (m, 1H), 2.47-2.51 (m, 4H), 2.71 (s, 3H), 2.77-2.88 (m, 2H), 3.03-3.10 (m, 2H), 3.12-3.21 (m, 4H), 3.37 (m, 1H), 7.14 (d, 2H), 7.15-7.32 (m, 5H), 7.88 (m, 2H).

Example 6

This Example illustrates the preparation of (R) N-(3-[3,5-difluorophenyl]-3-[1- methanesulphonylpiperidin-4-yl]propyli)-4-[2-(4-methanesulphonylphenylsulphonyl)- ethyl]piperidine (Compound 32 in Table III). 0

Fon

Q

N°

MP-triacetoxyborohydride (where MP stands for “macroporous”; 585 mg) was added to a solution of (R) 3-(1-methanesulphonylpiperidin-4-yl1)-3-[3,5- difluorophenyl]propionaldehyde (199 mg) (Method G) and 4-(2-[4- methanesulphonylphenylsulphonyl]ethyl)piperidine (194 mg) (Method B) in 20 ml of dichloromethane and the mixture was stirred at room temperature for 16 hours. The reaction mixture was filtered and the solid washed with dichloromethane (3x10 ml) and the combined dichloromethane filtrate and washings were poured onto a 25g bond Elut cartridge and eluted with a solvent gradient (ethyl acetate - 20% methanol/ethyl acetate) to give the title compound, yield 168 mg; MH" 647. NMR (DMSOd6) [note not all peaks are reported]: 2.78 (s, 3H), 6.87 (d, 2H), 6.99 (t, 1H), 8.14 (q, 4H).

Example 7

This Example illustrates the preparation of (R) N-(3-phenyl-3-[1- methanesulphonylpiperidin-4-yl]propyl)-4-[2-(4-methanesulphonyloxyphenylsulphonyl)- ethyl]piperidine (Compound 29 in Table III). 0) . 0) 4 0 on 2 0-4 oO

Methanesulphonyl chloride (60.3 mg) was added to a solution of (R) N-(3-phenyl-3- [1-methanesulphonylpiperidin-4-yl]propyl)-4-[2-(4-hydroxyphenylsulphonyl)ethyl]piperidine (Compound 28 in Table II; 290 mg) and triethylamine (53 mg) in dichloromethane (10 ml) and the mixture was stirred for 16 hours, then washed with saturated aqueous sodium bicarbonate (2x20 ml) and dried. The drying agent was filtered and the filtrate was poured onto a 20g Bond Elut cartridge and eluted with a solvent gradient (ethyl! acetate - 20% methanol/ethyl acetate) to give the product, yield 41.5 mg. NMR (DMSOQd6) [note not all peaks are reported]: 8 2.77 (s, 3H), 7.11-7.23 (m, 3H), 7.30 (t, 2H), 7.60 (d, 2H), 8.0 (d, 2H).

Example 8

This Example illustrates the preparation of (R) N-(3-phenyl-3-[1-methanesulphonyl- piperidin-4-yl]}propyl)-4-[2-(4-tetrazol-5-yl-phenylsulphonyl)ethyl]piperidine (Compound 30 in Table II). 0] aN! A.0 07 Shi S

N

TC,

N—p’

Ammonium chloride (67 mg) and sodium azide (81.6 mg) were added to a solution of (R) N-(3-phenyl-3-[1-methanesulphonylpiperidin-4-yl]propyl)-4-[2-(4-cyanophenyl- sulphonyl)ethyl]piperidine (350 mg; prepared by the method described in Example 6 using 4-

(2-[4-cyanophenylsulphonyl]ethyl)piperidine [Method B] as reactant) in DMF (10 ml) and the mixture was heated at 100°C for 8 hours. Further equivalents of ammonium chloride (67 mg) and sodium azide (81.6 mg) were added and the mixture was heated at 100°C for a further 8 hours. The solvent was evaporated and the residue was stirred with water (10 ml). The water - 5 was decanted and the residue was dissolved in methanol (10 ml) and poured on to a 20g

SCX2 cartridge eluted with methanol (4x20 ml) and 1M ammonia/methanol. The ammonia/methanol washings were evaporated to dryness to give the title compound, yield 140mg, MH" 601. NMR (DMSOd6) [note not all peaks are reported]: 52.77 (s, 3H), 7.04- 7.25 (m, 3H), 7.30 (t, 2H), 7.85 (d, 2H), 8.18 (d, 2H).

Example 9

Preparation of (4-{[2-(1-{(3R)-3~(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl} piperidin-4-yl)ethyl]sulfonyl} phenoxy)acetonitrile (Compound 15 of Table V ) and 2-(4-{[2-(1- {(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl} piperidin-4-yl)ethyl]sulfonyl} phenoxy)acetamide (Compound 16 of Table V'). 0:40 o.l.o

F N F ot a de Tee {4-[(2-Piperidin-4-ylethyl)sulfonyl phenoxy} acetonitrile (0.9g, Method M) was dissolved in a solution of (R)-3-(3,5-difluorophenyl)-3-(4-methanesulphonylphenyl)- propionaldehyde (0.85g) in dichloromethane (50 ml) and sodium triacetoxyborohydride (0.55g) was added. The reaction mixture was stirred for 16 hours, washed with 2M NaOH (2x50 ml), dried and evaporated to dryness. The residue obtained was purified by chromatography on a Bond-Elut column using an elution gradient of ethyl acetate - 30% methanol/ethyl acetate to give the title compound, yield 370 mg.

NMR (DMSOd6): 0.9-1.8 (m, 10H), 2-2.3 (m, 5H), 2.7 (m, 2H), 3.1 (5, 3H), 4.2 (t, 1H), 5.3 (s, 2H), 6.9-7.2 (m, 5H), 7.5-7.9 (m, 6H); MH 617.

A second fraction was collected and shown to be 2-(4-{[2-(1-{(3R)-3-(3,5- - difluorophenyl)-3-[4-(methylsulfonyl)phenyl]propyl} piperidin-4- yl)ethyl]sulfonyl}phenoxy)acetamide (Compound 16 of Table V), yield 168 mg.

NMR (DMSOd6): 0.9-1.8 (m, 10H), 2-2.3 (m, 5H), 2.7 (m, 2H), 3.1 (s, 3H), 4.2 (t, 1H), 4.65 (s, 2H), 6.9-7.2 (m, SH), 7.4-7.9 (m, 6H); MH 635.

Example 10 . 5 Preparation of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4-(methylsulfonyl)phenyl propyl} -4- (2-{[4-(1H-tetrazol-5-ylmethoxy)phenyl]sulfonyl} ethyl)piperidine (Compound 17 of Table

V) 0.l.0

F N ° N-N.

Ape

A mixture of (4-{[2-(1-{(3R)-33,5-difluorophenyl)-3-[4-(methylsulfonyl)phenyl]- propyl}piperidin-4-yl)ethyl}sulfonyl} phenoxy)acetonitrile (300 mg), sodium azide (63 mg) and ammonium chloride (52 mg) in DMF (10 ml) was stirred and heated at 100°C for 4 hours.

The solvent was evaporated and the residue was dissolved in water (10 ml). Water was decanted from the oil obtained and the residual oil was dissolved in methanol (10 ml) and : poured onto a 20g SCX2 cartridge and eluted with methanol (4x20 ml) and 1M ammonia/methanol (5x20 ml). The methanolic ammonia washings were evaporated to give the title compound, yield 0.15g. M'H 660. NMR (DMSOd6) [note not all peaks are reported]: 8 5.3 (s, 2H), 7.04 (t, 1H), 7.09-7.18 (m, 2H), 7.26 (d, 2H), 7.59 (d, 2H), 7.75 (d, 2H), 7.84 (d, 2H).

Example 11

Preparation of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4-(methylsulfonyl)phenyl]propyl} -4- (2-{[4-(2-methyl-2H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine (Compound 19 of Table

Vv) o.l.0 ® . o£, Q N= 4-(2-{[4-(2-methyl-2H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine (300 mg,

Method N) was added to a solution of (R)-3-(3,5-difluorophenyl)-3-(4-methanesulphonyl-

phenyl)propionaldehyde (290 mg) dissolved in dichloromethane (20 ml) followed by MP- triacetoxyborohydride (900mg) and the reaction mixture was stirred for 16 hours, filtered and evaporated to dryness. The residue was purified by chromatography on a Bond-elut column using an eluant gradient of ethyl acetate- 15% methanol/ethy] acetate to give the title . 5 compound, yield 167 mg.

NMR (CDCl): 1.2-1.4 (m, 3H), 1.6-1.9 (m, 7H), 2.8 (m, 2H), 3.05 (s, 3H), 3.1 (m, 2H), 4.1 (m, 1H), 4.05 (s, 3H), 6.7 (m, 3H), 7.4 (m, 2H), 7.85 (d, 2H), 8 (d, 2H), 8.39 (d, 2H),

M'H 644.

Using the procedure outlined above and using (R)-3-(1-methanesulphonylpiperidin-4- yl)-3-phenylpropionaldehyde (Method G) as starting material there was obtained 1- (methylsulfonyl)-4- {(1R)-3-[4-(2-{[4-(2-methy]-2 H-tetrazol-5-yl)phenyl] sulfonyl} ethyl)- piperidin-1-yl]-1-phenylpropyl} piperidine (Compound 20 of Table V), MH 615.

Example 12

Preparation of methyl (4- {[2-(1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl} piperidin-4-yl)ethyl sulfonyl} phenoxy)acetate (Compound 27 of Table V) 0=$:0 ® i

Cy oye

F 00

The product obtained on the reductive amination of (3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propanal (0.85g) with benzyl {4-[(2-piperidin-4- ylethyl)sulfonyl]phenoxy } acetate (1.1g; prepared according to Method M steps 1 and 2, using benzyl bromoacetate as starting material), carried out according to the method described in

Example 4, was poured onto a 20g SCX2 column and eluted with methanol (5x20 ml) and 10% ammonia in methanol (5x20 ml). The methanolic ammonia washings were concentrated and the product isolated had undergone ester exchange with the methanol eluant. The title compound was obtained, yield 1.3g; MH 650. :

NMR (CDCl): 1.2 (m, 4H), 1.6 (m, 6H), 1.8 (t, 2H), 2.8 (m, 2H), 3.01 (s, 3H), 3.1 (m, 2H), 3.8 (s, 3H), 4.1 (m, 1H), 4.7 (s, 2H), 6.6-6.8 (m, 3H), 7 (d, 2H), 7.4 (d, 2H), 7.8-7.9 (m, 4H). . 5 Example 13

Preparation of (4-{[2-(1-{(3R)-3-(3,5-difluorophenyl)-3-[4-(methylsulfonyl)pheny]]- propyl} piperidin-4-yl)ethyl]sulfonyl} phenoxy)acetic acid (Compound 28 of Table V). 0-80 xl ox

N 0] 00 2M NaOH was added to a solution of methyl (4- {[2-(1-{(3R)-3-(3,5-difluorophenyl)- 3-[4-(methylsulfonyl)phenyl]propyl} piperidin-4-yl)ethyl]sulfonyl} phenoxy)acetate (1.2g) (Example 12) in a mixture of ethanol (20 ml) and THF (20 ml) and the mixture was stirred for 2 hours. The reaction mixture was evaporated to dryness and water (10 ml) was added. The : solution was acidified to pH 3 with 2M HCI, the pH was adjusted to ~5 with sodium acetate and the mixture was extracted with dichloromethame (4x25 ml). The combined extracts were dried and evaporated to give the title compound, yield 0.9g. MH 636.

NMR (CDCl;) [note that not all peaks are reported]: 3.03 (s, 3H), 4.01 (t, 1H), 4.48 (bs, 2H), 6.67 (t, 1H), 6.73 (d, 2H), 7.40 (d, 2H), 7.73 (d, 2H), 7.86 (d, 2H). ‘ Example 14

Preparation of 2-(4-{[2-(1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl} piperidin-4-yl)ethyl]sulfonyl} phenoxy)-N- (methylsulfonyl)acetamide (Compound 29 Table V)

O= =O . ® .

Ny TR

F RS © -

EDCI was added to a solution of (4-{[2-(1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl}piperidin-4-yl)ethyl]sulfonyl} phenoxy)acetic acid

(400 mg), methanesuiphonamide (59 mg) and dimethylaminipyridine (163 mg) in dichloromethane and the mixture was stirred for 20 hours. The mixture was washed with ] water (2x25 ml), dried and evaporated to dryness. The residue was passed through a Bond-

Elut column eluting with a solvent gradient of ethyl acetate - 25% methanol/ethyl acetate to . 5 give the title compound as a white solid, yield 8.3 mg. M'H 713.

NMR (DMSOd6): [note that not all peaks are reported]: 4.18 (t, 1H), 4.42 (s, 2H), 6.97-7.12 (m, 3H), 7.15 (d, 2H), 7.59 (4, 2H), 7.74 (d, 2H), 7.85 (d, ZH).

Example 15

Preparation of 2-(4-{[2-(1- {(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl} piperidin-4-yl)ethyl]sulfonyl} phenoxy)-N-methylacetamide (Compound 30 Table V). 0=8-0

TOO

I 0

A mixture of (4-{ [2-(1-{(3R)-3-(3,5-difluorophenyl)-3-[4-(methylsulfonyl)phenyl}- propyl} piperidin-4-yl)ethyljsulfonyl}pbenoxy)acetic acid (400 mg), HOBT (85 mg) and

EDCI (245 mg) in dichloromethane (25 ml) was stirred at room temperature for 1.5 hours.

Methanolic ammonia (10 ml) was added and stirring was continued for 16 hours. The reaction mixture was washed with water (2x20 ml), dried and evaporated to dryness and the residue obtained was dissolved in dichloromethane (20 ml) and stireed with MP carbonate (1g) for 2 hours. The product was chromatograhed on a Bond-Elut column eluting with a solvent ‘gradient of ethyl acetate- 10% methanol/ethyl acetate to give the title compound, yield 144 mg. MH 649.

NMR (CDCl): 1.2 (m, 4H), 1.6 (m, 6H), 1.8 (t, 2H), 2.8 (m, 2H), 2.95 (4, 3H), 3.01 - (s, 3H), 3.1 (m, 2H), 4.1 (m, 1H), 4.6 (s, 2H), 6.5 (broad peak, 1H), 6.6-6.8 (m, 3H), 7.05 (4, 2H), 7.4(4, 2H), 7.9 (m, 4H).

Example 16

Preparation of 1-{(1S,3(R or S)-3-(3,5-difluorophenyl)-1-methyl-3-[4- (methylsulfonyl)phenyl]propyl}-4-(2- {[4-(methylsulfonyl)phenyl] sulfonyl} ethyl)piperidine and 1-{(15,3(S or R)-3-(3,5-difluorophenyl)-1-methyl-3-[4-(methylsulfonyl)phenyl}propyl}- . 5 4-(2-{[4-(methylsulfonyl)phenyl}sulfonyl}ethyl)piperidine —

Ww,

Oa

F ) pa a

IN"

To stirred solution of (4R)-4~(3,5-difluorophenyl)-4-[4-(methylsulfonyl)phenyl]butan- 2-one (500mg) in THF (50ml) was added 2 equivalents of 4-(2-{[4- (methylsulfonyl)pheny!Jsulfonyl}ethyl)piperidine and Sm titanium isopropoxide, then stirred for 1 hour at 20-25°C. Sodium tris-acetoxyborohydride (2.5 g) was then added and stirring continued for 16 hours, then 2N NaOH (10mi) was added and the organic layer decanted from the white precipitate. The inorganic solids were slurried again with THF and the combined organic layers were dried and evaporated. The crude product was subjected to chromatography on silica, eluting with ethyl acetate to give a pure sample of one of the diastereomers (yield 30 mg).

NMR (CDCl): 0.91 (d, 3H), 1.1-2.6 (m, 13H), 2.77 (m, 1H), 3.04 (s, 3H), 3.12 (s, 3H), 3.16 (m, 2H), 4.25 (m, 1H), 6.66 (t, 1H), 6.76 (d, 2H), 7.40 (d, 2H), 7.86 (d, 2H), 8.12 (d, 2H), 8.18 (d, 2H).

Example 17

Preparation of the hydrochloride salt of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4- : (methylsulfonyl)phenyl]propyl}-4-(2-{[4-(methylsulfonyl)phenyl]sulfonyl} ethyl)piperidine. 4M HCl in dioxane (0.08 ml) was added to a hot solution of 1-{(3R)-3-(3,5- difluorophenyl)-3-[4-(methylsulfonyl)phenyl propyl} -4-(2- {[4-(methylsulfonyl)phenyl]- sulfonyl}ethyl)piperidine (0.2g) in ethanol (25 ml) and the solution was allowed to cool and stand at room temperature for 16 hours. The hydrochloride salt obtained was filtered and dried, yield 197 mg. A sample of the salt obtained was crystallized from ethanol, filtered and dried. PXRD of this compound is presented in Figure 1.

Example 18 } 5 Preparation of the maleate salt of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl}-4-(2- {[4-(methylsulfonyl)phenyl]sulfonyl} ethyl)piperidine. 1-{(3R)-3-(3,5-Difluorophenyl)-3-[4-(methylsulfonyl)phenyl]propyl}-4-(2-{[4- (methylsulfonyl)phenyl]sulfonyl}ethyl)piperidine (3g) was dissolved in a mixture of ethyl acetate (50ml) and ethanol (25ml) at 50°C. Meanwhile maleic acid (0.6g) was dissolved in ethanol (25ml) at 50°C and, when both solutions were ready, the solution of maleic acid was poured into the solution of free base. The resulting mixture was stirred while allowing to cool and then filtered after 1hour and the residue (title compound) washed with ethyl acetate. The residue was dired in a vacuum oven to leave the title compound (about 3.5g, approximately 95% yield). PXRD of this maleate salt is presented in Figure 2. :

The succinate, malonate and fumarate salts of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl}-4-(2- {[4-(methylsulfonyl)phenyl]sulfonyl}ethyl)piperidine : were prepared using the method of Example 18. The fumarate salt of 1-{(3R)-3-(3,5- difluorophenyl)-3-[4-(methylsulfonyl)phenyl]propyl}-4-(2-{[4- (methylsulfonyl)phenyl]sulfonyl}ethyl)piperidine was formed as a crystalline solid. The tartrate salt was formed as a gum.

PXRD of the succinate salt of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl}-4-(2- {[4-(methylsulfonyl)phenyl]sulfonyl} ethyl)piperidine is presented in Figure 3.

PXRD of the malonate salt of 1-{(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyl]propyl} -4-(2- {{4-(methylsulfonyl)phenyl}sulfonyl} ethyl)piperidine is presented in Figure 4.

Preparation of certain intermediates is now presented in Methods A to U.

Method A (S)-3-Phenyl-3-(4-methanesulfonylphenyl)propionaldehyde

MeO_S hr

Step 1: Preparation of (4S, 5R)-1-[(S)-3-(4-methanesulfonyl-phenyl)-3-phenyl-propionyl]}-3,4- dimethyl-5-phenyl-imidazolidin-2-one

Dus jens ey

MeO,S oh

To a mixture of copper (I) iodide (960mg, 5.0mmol) and THF (20mL) was added

N,N,N’,N’-tetramethylethylenediamine (0.83mL, 5.5mmol) and the resulting mixture was stirred at room temperature for 10min. then cooled to —78°C. Phenylmagnesium bromide (5.0mL, IM in THF, 5.0mmol) was added and the resulting mixture stirred at 78°C for 15min. A solution of di-n-butylboron triflate (3.0mL, 1M in diethyl ether, 3.0mmol) and (£)- (4S, 5R)-1-(3-[4-methanesulfonylphenyl]acryloyl)-3,4-dimethyl-5-phenyl-imidazolidin-2-one (step 4 below), 1.0g, 2.51mmol) in THF (15mL) was added and the resulting mixture was stirred whilst allowing to warm to room temperature for 18h. The reaction mixture was washed with saturated aqueous ammonium chloride, water and brine, dried (MgSO) and evaporated. The residue was purified by eluting through a 20g Bond Elut with gradient of isohexane to ethyl acetate giving the sub-titled compound (1.49g, 100%); NMR (CDCl3): 0.78 ] (d, 3H), 2.82 (s, 3H), 3.00 (s, 3H), 3.78 (dd, 1H), 3.80 (m, 1H), 3.98 (dd, 1H), 4.72 (m, 1H), 5.19 (d, 1H), 6.99 (m, 2H), 7.22 (m, 8H), 7.48 (d, 2H), 7.79 (d, 2H); MS: 477 (MH+). . 20

Step 2: Preparation of (S)-3-phenyl-3-(4-methanesulphonylphenyl)propan-1-ol

To a solution of (4S, 5R)-1-[(S)-3-(4-methanesulphonyl-pheny!)-3-phenyl-propionyl]- 3,4-dimethyl-5-phenyl-imidazolidin-2-one (846mg, 1.78mmol) in THF (20mL) at 0°C was added lithium aluminium hydride (3.6mL, IM in THF, 3.6mmol) and the resulting mixture was stirred for 15min. The reaction was quenched by the addition of 2M aqueous sodium hydroxide. The phases were separated and the organic phase pre-absorbed onto a Bond Elut and eluted with a gradient of isohexane to ethyl acetate giving the sub-titled compound as a ; 5 white solid (285mg, 55%); NMR (CDCl): 1.63 (brs, 1H), 2.33 (m, 2H), 3.00 (s, 3H), 3.59 (t, 2H), 4.28 (t, 1H), 7.23 (m, 5H), 7.43 (d, 2H), 7.82 (d, 2H).

Step 3: Preparation of the title compound

To a solution of (S)-3-phenyl-3-(4-methanesulfonylphenyl)propan-1-ol (244mg, 0.84mmol) in DCM (5mL) was added Dess-Martin periodinane (392mg, 0.92mmol) and the resulting mixture was stirred at room temperature for 1.5h. The mixture was washed with 2M aqueous sodium hydroxide (2 x 10mL), dried and evaporated to give the title compound.

Step 4: Preparation of E-(4S, 5R)-1-(3-[4-Methanesulphonylphenyl]acryloyl)-3,4-dimethyl-5- phenyl-imidazolidin-2-one : o O rr

MeO,S SO

To a stirred solution of 3-(4-methanesulphonylphenyl)acrylic acid (7.14g, 31.5mmol) in DCM (10mL) was added thionyl chloride (3mL, 34.7mmol) dropwise and the resulting mixture was stirred at room temperature for 18h. To this solution was added DIPEA (5.04mL, 28.9mmol) dropwise at room temperature. The resulting solution was added to a stirred solution of (4R, 55)-1,5-dimethyl-4-phenyl-imidazolidin-2-one (5.0g, 26.3mmol) in DCM (20mL) and DIPEA (4.58mL, 26.9mmol) and the resulting mixture stirred at room temperature for 4h. The mixture was washed with water and brine, pre-absorbed onto a Bond : Elut and eluted with a gradient of isohexane to ethyl acetate giving the title compound as a solid (7.61g, 73%); NMR (CDCl,): 0.84 (d, 3H), 2.89 (s, 3H), 3.04 (s, 3H), 3.98 (m, 1H), 5.42 : (d, 1H), 7.20 (m, 2H), 7.32 (m, 3H), 7.69 (d, 1H), 7.74 (d, 2H), 7.93 (d, 2H), 8.31 (4, 1H);

MS: 399 (MH+).

Method B

4-[2-(4-Methanesulphonylphenylsulphonyl)ethyl]piperidine

AP

SHAG

: 0

N i Z

Step 1 Preparation of N-tert-butoxycarbonyl-4-[2-(4-methylthiophenylthio)ethyl]piperidine ~ Ss 0) Sh Tl

T

4-Methylthiobenzenethiol (1.16g) was added to a suspension of sodium hydride (297mg of 60% dispersion in mineral oil) in DMF (20ml) at 0°C and stirred at this temperature for 30 minutes. N-tert-Butoxycarbonyl-4-[2-(4-toluenesulphonyloxy)ethyl]- piperidine (CAS No. 89151-45-1) (2.84g) was added, the reaction mixture was allowed to warm to room temperature and was stirred for 16 hours. The reaction mixture was evaporated to dryness and the residue obtained was dissolved in dichloromethane (30 mi) and the solution was washed with water (20 ml) and brine (20 ml) and dried. The residue obtained on removal of the solvent was chromatographed on a 50g silica Bond Elut column eluting with a solvent gradient of isohexane - 50% ethyl acetate/isohexane. Yield 2.5g, MH" 268.

Step 2 Preparation of N-tert-butoxycarbonyl-4-[2-(4-methylsulphonylphenylsulphonyl)ethyl]- piperidine. ~ Q 7

LT Cle m-Chloroperbenzoic acid (5.64g) was added to a solution of N-tert-butoxycarbonyl-4- [2-(4-methylthiophenylthio)ethyl]piperidine (2.1g) in dichloromethane (90 ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 16 hours. The ; reaction mixture was washed with saturated aqueous sodium bicarbonate solution (20 ml), water (20 ml) and brine (20 ml) then dried and evaporated to dryness. The product was chromatographed on a 50g silica Bond Elut column eluting with a solvent gradient of 20% ethyl acetate/isohexane - ethyl acetate to give the product, yield 1.82g, MH" 375.9.

Step 3 Preparation of title compound

Variant A

Trifluoroacetic acid (5 ml) was added to a solution of N-terz-butoxycarbonyl-4-{2-(4- methylsulphonylphenylsulphonyl)ethyl]piperidine (1.94g) in dichloromethane (20 ml) and was allowed to stand at room temperature for 1 hour. The reaction mixture was evaporated to ) 5 dryness and the residue was dissolved in 2M sodium hydroxide (15 ml) and extracted with dichloromethane (3x20 ml). The combined dichloromethane extracts were dried and evaporated to dryness to give the title compound, yield 1.3g, MH'331.9.

Variant B

A solution of 4M hydrochloric acid in dioxane (15 ml) was added to a stirred solution of N-tert-butoxycarbonyl-4-[2-(4-methylsulphonylphenylsulphonyl)ethyl]piperidine (5.62g) in dichloromethane (15 ml) and stirring was continued for 1 hour. The reaction mixture was triturated with diethyl ether and the solid formed was filtered, washed with diethyl ether and dried under high vacuum. The title compound was obtained as its hydrochloride salt, yield 4.88g, M'H 331.9.

The following compounds were also prepared using a process analogous to Method B

Ny

S

"Oh OW

RE LE

)

oO, °

Ssg?

ER LS

Method C 3-Phenyl-3-(N -methanesulphonylpiperidin-4-yl)propionaldehyde 3 "Cy 20

Step 1 Preparation of 4-benzoyl-1-methanesulphonylpiperidine

Nl oY ) oO

Methanesulphony! chloride was added to a stirred slurry of 4-benzoylpiperidine hydrochloride (4.51g) and triethylamine (8.35ml) in dichloromethane (100ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 16 hours. The . mixture was diluted with dichloromethane (50ml) and washed with ammonium chloride solution (2x25ml) and brine (25ml), dried and evaporated to dryness to give 4-benzoyl-1- methanesulphonylpiperidine as a white solid, yield 3.98g. NMR (CDCls): 1.93 (m, 4H), 2.81 (s, 3H), 2.98 (d-t, 2H), 3.40 (m, 1H), 3.77 (m, 2H), 7.43 (t, 2H), 7.57 (1, 1H), 7.89 (d, 2H).

Step 2 Preparation of ethyl 3-phenyl-3-(N-methanesuiphonylpiperidin-4-yl)acrylate.

SI

0“™™N

AN On “xv

Lithium bis(trimethylsilyl)amide (16.3ml of 2 1M solution in THF) was added dropwise to a solution of triethylphosphonoacetate (2.93ml) in THF at 0°C under an argon atmosphere and the mixture was stirred for 30 minutes. A slurry of 4-benzoyl-1- methanesulphonylpiperidine (3.96g) in THF (30ml) was added, the reaction mixture was allowed to warm to room temperature and stirring was continued for 24 hours. The reaction mixture was diluted with dichloromethane (80ml) and water (80ml). The organic layer was washed with water and the combined aqueous extracts were in turn extracted with dichloromethane (50ml). The combined dichloromethane extracts were washed with brine (25m), dried and evaporated to dryness. The residue was chromatographed on a 90g Biotage column eluted with a solvent gradient (30-5% ethyl acetate/isohexane to give a less polar fraction (1.62g) and a more polar fraction (0.53g). Both fractions (cis/trans isomers) were combined and used for the next step.

Less polar NMR (CDCls): 1.27 (t, 3H), 1.69 (m, 2H), 1.81 (d, 2H), 2.72 (s, 3H), 2.72 (t, 2H), 3.81 (d, 2H), 3.88 (m, 1H), 4.21 (q, 2H), 5.78 (s, 1H), 7.11 (m, 2H), 7.27 (m, 3H).

More polar NMR (CDCl): 1.01 (t, 3H), 1.56 (m, 2H), 1.85 (d, 2H), 2.31 (m, 1H), 2.63 (t, 2H), 2.74 (s, 3H), 3.83 (d, 2H), 3.92 (q, 3H), 5.82 (s, 1H), 7.04 (d, 2H), 7.30 (m, 3H).

Step 3 Preparation of ethyl 3-phenyl-3-(N-methanesulphonylpiperidin-4-yl)propionate 3S 0”> Nn oO eS

A solution of ethyl 3-phenyl-3-(N-methanesulphonylpiperidin-4-yl)acrylate (2.06g) in ethanol (30ml) was hydrogenated over 24 hours under a hydrogen filled balloon using 20% palladium hydroxide as catalyst. The reaction mixture was filtered through Celite and the filtrate evaporated to dryness. The product obtained was used for the next step without further purification. MH"340.

Step 4 3-Phenyl-3-(N-methanesulphonylpiperidin-4-yl)propan-1-ol.

J

OH

A solution of ethyl 3-phenyl-3-(N-methanesulphonylpiperidin-4-yl)propionate (2g) in

THF (10ml) was added to a suspension of lithium aluminium hydride (232mg) in THF (20ml) at 0°C under argon over 30 minutes. The reaction mixture was allowed to warm to room temperature and stirred for 2 hours. Water (10ml) was added followed by magnesium sulphate (10g). The reaction mixture was filtered and the filtrate evaporated to dryness to give the product as a white foam, yield 1.57g. NMR (CDCl;): 1.40 (m, 4H), 1.57 (m, 1H), 1.78 (m, 1H), 2.01 (m, 2H), 2.45 (m, 2H), 2.58 (t, 1H), 2.70 (m, 3H), 3.31 (m, 1H), 3.42 (m, 1H), 3.67 (d, 1H), 3.80 (d, 1H), 7.04 (d, 1H), 7.19 (t, 1H), 7.29 (q, 2H).

Step 5 Preparation of the title compound <3

Dess-Martin periodinane (739mg) was added to a stirred solution of 3-phenyl-3-(N- methanesulphonylpiperidin-4-yl)propan-1-ol (454mg) in dichloromethane (8m!) and stirring was continued for 2 hours. The reaction mixture was diluted with dichloromethane (100ml) 20 and washed with 2M sodium hydroxide (2x50ml), brine (50ml) and dried. The product obtained on removal of the solvent was used in subsequent steps without purification.

Method D

N-(3-Phenyl-3-chloropropyl)-4- [2-(4-methanesulphonylphenylsulphonyl)ethyl]-piperidine cl

A adi

N°

Triethylamine (0.73 ml) was added to a solution of N-(3-hydroxy-3-phenylpropyl)-4- [2-(4-methanesulphonylphenylsulphonyl)ethyl]-piperidine (1.22g) in dichloromethane (20 ml) followed by methanesulphonyl chloride (0.33g) and the mixture was stirred for 18 hours at room temperature. The reaction mixture was washed successively with water (25 ml) and brine (25 ml) and dried. The residue obtained after removal of the solvent was chromatographed on a 20g silica Bond Elut column eluted with a solvent gradient of ethyl acetate - 20% methanol/ethy! acetate to give the title compound, yield 0.73g, MH" 483.99.

NMR(CDCls) : 1.3 (m, 3H), 1.6 (m, 4H), 1.9 (m, 2H), 2.1-2.3 (m, 2H), 2.4 (m, 2H), 2.8-2.9 (m, 2H), 3.1 (s, 3H), 3.2 (m, 2H), 5.0 (m, 1H), 7.3 (m, 5H), 8.2 (m, 4H).

N-(3-Hydroxy-3-phenylpropyl)-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]-piperidine : : HO, is AN

Sodium borohydride (100mg) was added in portions to a solution of N~(3-oxo-3- phenylpropyl)-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]-piperidine (1.22g) in ethanol (20ml) at room temperature and was stirred for 18 hours. The reaction mixture was evaporated to dryness and the residue was dissolved in dichloromethane (30ml) and this . 20 solution was washed with water (25ml), brine (25ml) and dried. Removal of the solvent gave the title compound as white solid, yield 1.21g, MH" 465.98.

N-(3-Ox0-3 -phenylpropyl)-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]-piperidine lo

A Oto ay 3-Chloropropiophenone (0.726g) was added to a mixture of 4-[2-(4- methanesulphonylphenylsulphonyl)ethyl]-piperidine (1.3g) (prepared as described in Method

B) and potassium carbonate (1.09g) in DMF (20m) and the mixture was stirred at room temperature for 1 hour. The reaction mixture was evaporated to dryness and the residue was dissolved in dichloromethane (30ml). The dichloromethane solution was washed with water (25m), brine (25ml) and dried. The residue obtained after removal of the solvent was chromatographed on a 50g silica Bond Elut column eluted with a solvent gradient of ethyl acetate-20% methanol/ethyl acetate to give the title compound as a white solid, yield 1.22g,

MH" 463.97. NMR(CDCly): 1.2-1.4 (m, 3H), 1.6 (m, 4H), 2.0 (m, 2H), 2.8 (m, 2H), 2.9 (m, 2H), 3.1-3.2 (m 7H), 7.4 (m, 2H), 7.5(m, 1H), 7.9 (m, 2H), 8.2 (m, 4H).

Method E (R)-3-(3,5-Difluorophenyl)-3-(4-methanesulfonylphenyl)propionaldehdye

MeO,S ry

F 9 F

This was prepared from (4S, 5R)-1-(3-[4-methanesulfonylphenyljacryloyl)-3,4- dimethyl-5-pbenyl-imidazolidin-2-one and 3,5-difluorophenylmagnesium bromide using a method similar to that used to prepare (5)-3-phenyl-3-(4-methanesulfonyl- phenyl)propionaldehyde from phenylmagnesium bromide (Method A); NMR (CDCl): 3.05 (s, 3H), 3.20 (d, 2H), 4.72 (t, 1H), 6.75 (m, 3H), 7.35 (d, 2H), 7.88 (d, 2H), 9.75 (s, 1H).

Method F (S) N-(3 -hydroxy-3-phenylpropyl)-4-[2-(4-fluorophenylsulphonyl)ethyl]-piperidine

Step 1: (R or S)N-(3 -chloro-3-phenylpropyl)-4-[2-(4-fluorophenylsulphonyl)ethyl]- . 5 piperidine cl shaoUles &o

Methanesulphonyl chloride (158 pl) was added to a solution of (S) N-(3-hydroxy-3- phenylpropyl)-4-[2-(4-fluorophenylsulphonyl)ethyl]-piperidine (600 mg) and triethylamine (417 pl) in dichloromethane (10 ml) maintained at 0°C under an argon atmosphere. The reaction mixture was allowed to warm to room temperature and was stirred for 18 hours. The reaction mixture was diluted with dichloromethane (50 ml) and washed with saturated amonium chloride solution (2x25 ml) and brine (25 ml) and dried. Removal of the solvent gave the title compound which was used without further purification. NMR: (CDCl;): 1.18- 2.24 (m, 13H), 2.78 (m, 1H), 2.84 (m, 1H), 3.04 (1H, m), 4.92 (t, IH), 7.20-7.40 (m, 7TH), 7.91 (m, 2H); MS 424 (MH).

Step 2: (S) N-(3-hydroxy-3-phenylpropyl)-4-[2-(4-fluorophenylsulphonyl)ethyl]-piperidine

OH : saaeUNey &o

A solution of (S)-1-phenyl-3-(4-toluenesulphonyl)oxypropan-1-ol (459 mg) in dioxane (10ml) was added to a suspension of 4-[2-(4-fluorophenylsulphonyl)ethyl]piperidine (407 mg) and potassium carbonate (414 mg) and the mixture was heated at 95°C for 17 hours.

The reaction mixture was allowed to cool and was partitioned between dichloromethane (100 ml) and water (50 ml). The organic layer was collected and washed with water (50 ml), brine (50 ml) and dried. Removal of the solvent gave the title compound, yield 607 mg. NMR: (CDCl): 1.18-1.69 (m, 8H), 1.82 (m, 3H), 2.02 (m, 1H), 2.48 (m, 1H), 2.62 (m, 1H), 2.93 (d, 1H), 3.05 (m, 3H), 4.89 (m, 1H), 7.21-7.40 (m, 7H), 7.92 (m, 2H); MS 406 (MHD).

Method G (R) 3-(1-Methanesulphonylpiperidin-4-yl)-3-[3,5-difluorophenyl]propionaldehyde \ oO 0=¢ \

N

—0

F

F

Step 1 3-[N-(Benzyloxycarbonylpiperidin-4-yl)]propenoic acid

X

0) N % OH 10)

A mixture of N-benzyloxycarbonyl-4-formylpiperidine (10g), malonic acid (4.2), pyridine (4 ml) and piperidine (0.4 mi) was heated at 100°C for 2 hours. The reaction mixture was allowed to cool and was diluted with ethyl acetate (100 ml). The solution was washed with 2M HCl (2x100 ml), dried and evaporated to dryness. The residue was triturated with isohexane to give the title compound, yield 13.5g.

NMR (DMSO0d6): 1.2 (m, 2H), 1.7 (m, 2H), 2.35 (m, 1H), 2.85 (m, 2H), 4 (d, 2H), 5.05 (s, 2H), 5.75 (d, 1H), 6.75 (m, LH), 7.35 (m, SH), 12.25 (broad peak, 1H).

Step 2 N-(Benzyloxycarbonylpiperidin-4-yl)propenoic acid isopropyl ester

X

0” ON 0

A solution of N-(benzyloxycarbonylpiperidin-4-yl)propenoic acid (52g) in isopropanol (500 ml) containing concentrated sulphuric acid (20 ml) was heated under reflux for 32 hours. The solvent was evaporated and the residue was dissolved in ethyl acetate (250 ml). The ethyl acetate solution was washed with water (2x250 ml) and saturated aqueous sodium bicarbonate (2x25 ml) and dried. The residue obtained on evaporation of the solvent was chromatographed on a Bond Elut cartridge eluted with a solvent gradient (isohexane - 25% ethyl acetate/isohexane) to give the title compound, yield 54g.

Step 3 Preparation of (R) 3-(N-benzyloxycarbonylpiperidin-4-y1)-3-(3,5- difluorophenyl)propanoic acid isopropyl ester

OX

N hoot

F 0)

F

Dioxane (100 ml) was charged to a 500 ml three necked flask and purged with argon for 10 minutes. Acetylacetonatobis[ethylene]rhodium (I) (620 mg) and R-BINAP were added and the mixture was stirred for 10 minutes. 3,5-Difluorophenylboronic acid (19g) was added and the mixture was purged with argon for 10 minutes. N-(benzyloxycarbonylpiperidin-4- yl)propenoic acid isopropyl ester (8 g) and ethanediol (20 ml) in dioxane (100 ml) were added and the mixture was purged with argon for 10 minutes. The mixture was heated at 100°C for 18 hours, allowed to cool and was passed through activated alumina (200g) washed through with ethyl acetate (3x100 ml). The combined washings were evaporated to dryness and the residue obtained was dissolved in ethyl acetate (100 ml) and washed successively with saturated aqueous sodium bicarbonate (2x100 ml) and 2M HCl (2x100 ml), dried and evaporated to dryness. The product obtained (12g) was shown to be 40% of the required material by NMR and was used without further purification in the subsequent reactions.

Step 4 Preparation of (R) 3-(piperidin-4-yl)-3-(3,5-difluorophenyl)propanoic acid isopropyl ester.

N

> hoot

F lo)

F

A solution of (R) 3-(N-benzyloxycarbonylpiperidin-4-y1)-3-(3,5- difluorophenyl)propanoic acid isopropyl ester (12g) in ethanol (300 ml) containing 20%

palladium hydroxide on charcoal (2g) was hydrogenated under a hydrogen filled balloon.

The catalyst was filtered and the filtrate was evaporated to dryness to give the title compound (10g) which was used without further purification. : 5 Step 5 Preparation of (R) 3-(N-methanesulphonylpiperidin-4-yl)-3-(3,5-difluorophenyl)- propanoic acid isopropy! ester. — \ peets

F o s

Methanesulphony] chloride (3.7g) was added to a solution of (R) 3-(piperidin-4-yl)-3- (3,5-difluorophenyl)propanoic acid isopropyl ester (10g) and triethylamine (3.89g) in dichloromethane (100 ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was washed with 2M HCl (2x50 ml) and saturated aqueous sodium bicarbonate (2x50 ml), dried and evaporated to dryness to give the title compound (10g) which was used without further purification.

Step 6 Preparation of (R) 3-(N -methanesulphonylpiperidin-4-yl)-3-(3,5- difluorophenyl)propanol oY

N

: —OH

F

F

Lithium aluminium hydride (25 ml of a IM solution in THF) was added dropwise over 15 minutes to a solution of (R) 3-(N-methanesulphonylpiperidin-4-yl)-3-(3,5- difluorophenyl)propanoic acid isopropyl ester (10g) in THF (150 ml) at -10°C. The reaction mixture was stirred at 10°C for 30 minutes, 2M NaOH (25 ml) was added, the mixture was filtered and the filtrate evaporated to dryness. The residue obtained was dissolved in ethyl acetate and washed with 2M HCI (2x100 ml) and dried. The residue obtained on removal of the solvent was chromatographed on a Bond Elut column eluting with a solvent gradient (80% ethyl acetate/isohexane - ethyl acetate) to give the title compound, yield 2.2 g.

NMR (DMSO dé): 0.95-1.2 (m, 2H), 1.3 (m, 1H), 1.6 (n.2H), 1.9 (m, 2H), 2.6 (m, 2H), 2.8 (s, 3H), 3.1 (m, 1H), 3.2 (m, 1H), 3.4 (m, 1H), 3.5 (m, 1H), 6.8-7 (m, 3).

Step 7 Preparation of (R) 3-(N-methanesulphonylpiperidin-4-yl)-3-(3,5- diftluorophenyl)propionaldehyde. 0

N a

E

E

Dess-Martin periodinane (1g) was added to a solution of (R) 3-(N- methanesulphonylpiperidin-4-yl)-3-(3,5-difluorophenyl)propanol (0.8g) in dichloromethane (40 ml) and the mixture was stirred for 1.5 hours. The reaction mixture was washed with 2M

NaOH (2x20 ml) and dried. The solution of the title compound in dichloromethane was used in subsequent reactions.

Method H (R) 3-(N-Methanesulphonylpiperidin-4-y!)-3-phenylpropanol of

N

Step 1 Preparation of 3-(N-methanesulphonylpiperidin-4-yl)propenoic acid acid chloride.

Ni “0

ZZ Ci

Q

1-Chloro-N,N,2-trimethylpropenylamine (1.06 ml) was added dropwise over 10 minutes to a suspension of 3-(N-methanesulphonylpiperidin-4-yl)propenoic acid (1.86g, prepared from N-methanesulphonylpiperidine-4-carboxaldehyde [CAS 241 134-35-0] according to step 1 of Method E) in THF (20 ml) under an atmosphere of argon and the mixture was stirred for 2 hours and used directly in step 2.

Step 2 Preparation of 1-[3-(N -methanesulphonylpiperidin4-yl)propenyl}-(4S, 5R)-3,4- dimethyl-4-phenyl-imidazolidin-2-one. <0 o. / ” |S = N i is

Lithium bis(trimethylsilyl)amide (8 ml of a 1M solution in THF) was added dropwise to a suspension of (4R,5S)-1,5-dimethyl-4-phenyl-2-imidazolidinone (1.52g) in THF (20 ml) under argon at 10°C. The reaction mixture was stirred at —10°C for 10 minutes, allowed to warm to 0°C and maintained at this temperature for 10 minutes then cooled again to -10°C.

The solution of the acid chloride prepared in Step 1 was added dropwise and the reaction mixture was allowed to warm to room temperature and washed with water (100 ml). The aqueous extract was extracted with ethyl acetate (3x50 ml) and the ethyl acetate extracts were dried and the residue passed through a 90g Biotage column eluting with a solvent gradient (50% ethyl acetate/isohexane - 70% ethyl acetate/isohexane). Yield 1.89g. LC-MS MH" 406,

NMR (CDCl,): 0.8 (d, 3H), 1.5-1.6 (m, 3H), 1.9 (m, 2H), 2.3 (m, 1H), 2.7 (m, 2H), 2.75 (s, 3H), 2.8 (s, 3H), 3.75 (m, 2H), 3.9 (m, 1H), 5.3 (d, 1H), 6.85 (d-d, 1H), 7.1 (d, 1H), 7.2-1.35 : (m, 3H), 7.45 (4, 1H).

Step 3 Preparation of (R) 1-[3-phenyl-3-(methanesulphonylpiperidin-4-yl)propionyl}- (4S,5R)-3,4-dimethyl-5-phenyl-imidazolidin-2-one.

Ni o. .[/ 0” N YN

N

“5r

A mixture of copper(l) iodide (1.78 g) and NN,N'N ’-tetramethylethylenediamine (1.41 ml) in THF (50 ml) was stirred under argon for 1 hour then cooled to —78°C and phenylmagnesium bromide (5.4 ml of a IM solution in THF) was added and the mixture was stirred at —78°C for 30 minutes. A solution of 1-[3-(N-methanesulphonylpiperidin-4- yDpropenyl]-(4S, SR)-3,4-dimethyl-5 -phenyl-imidazolidin-2-one (1.89g) and dibutylboron triflate (4.67 ml of a 1M solution in diethylether) in THF (50 ml) was added over 10 minutes and the reaction mixture was stirred at —78°C for 1 hour then allowed to warm to room temperature. The reaction mixture was concentrated and filtered through a pad of silica (50g) washed with ethyl acetate (2x50 ml) and the ethyl acetate washings were washed with 2M

HCl (2x150 ml) and dried. The residue obtained on removal of the solvent was passed through a 90g Biotage column eluting with a solvent gradient (50% ethyl acetate/isohexane- 70% ethyl acetate/isohexane) to give the product as a yellow solid, yield 1.34g, MH" 484.

NMR (CDCl): 0.7 (d, 3H), 1.2 (m, 1H), 1.35 (m, 1H), 1.5 (m, 1H), 1.9 (m, 1H), 2.45 (m, 1H), 2.55 (m, 1H), 2.7 (s, 3H), 2.8 (s, 3H), 3.1 (m, 1H), 3.2 (d-d, 1H), 3.4 (m, 1H), 3.65 (m, 1H), 3.75-3.9 (m, 3H), 5.2 (d, 1H), 6.7 (d, 2H), 7.05-7.25 (m, 8H).

Step 4 Preparation of the title compound

A solution of (R) 1-[3 -phenyl-3-(methanesulphonylpiperidin-4-yl)propionyl}-(4S,5R)- 3,4-dimethyl-5-phenyl-imidazolidin-2-one (1.34g) in THF (14 ml) was added to a solution of lithium aluminium hydride (2.77 ml of a 1M solution in THF) in THF (10 ml) at 0°C and the mixture was allowed to warm to room temperature over 1 hour. Water (5 ml) was added cautiously, then THF (15 ml) and solid magnesium sulphate. The reaction mixture was filtered and the filtrate was passed through a 40 g Biotage column eluted with a solvent gradient (50% ethyl acetate/isohexane - 70% ethyl acetate/isohexane) to give the title compound as a white solid, yield 338 mg. NMR (CDCl): 1.15-1.25 (m, 2H), 1.3-1.5 (m, 2H), 1.6 (m, 1H), 1.75 (m, 1H), 1.95-2.10 (m, 2H), 2.5 (m, 2H), 2.6 (m, 1H), 2.7 (s, 3H), 3.3-3.4 (m, 2H), 3.45 (m, 1H), 3.7 (m, 1H), 3.85 (m, 1H), 7.05 (m, 2H), 7.15-7.35 (m, 3H).

Method 1

Preparation of [(piperidin-4-yl)methyl]-(4-methoxyphenylmethyl)sulphone

OMe :

Step 1 Preparation of [(N-Boc-piperidin-4-yl)methyl]-(4-methoxyphenylmethyl)sulphide

JC

8) N 1 hl OMe

Oo 4-Methoxybenzylthiol (0.944 ml) was added to a suspension of sodium hydride (271 mg of a 60% dispersion in mineral oil) in DMF at 0°C and was stirred at this temperature for 15 minutes. 4-Tosyloxymethyl-N-Boc-piperidine (CAS 166815-96-9) was added and the mixture was allowed to warm to room temperature and was stirred for 3 hours. The reaction mixture was evaporated to dryness and the residue was dissolved in dichloromethane (50 ml) and this solution was washed with water (30 ml) and brine (30 ml) and dried. The solvent was evaporated and the residue was chromatographed on a 50g Bond Elut column eluting with a solvent gradient (isohexane-20% ethyl acetate/isohexane). Yield 2g, MH 252.14.

NMR (CDCl): 1.0-1.2 (m, 2H), 1.4 (s, 9H), 1.5 (m, 1H), 1.7-1.8 (m, 2H), 2.3 (m, 2H), 2.6 (bt, 2H), 3.7 (s, 2H), 3.8 (s, 3H), 4.1 (m, 2H), 6.8 (m, 2H), 7.2 (m, 2H).

Step 2 Preparation of [(N-Boc-piperidin-4-yl)methyl]-(4-methoxyphenylmethyl)sulphone

S spot FL, 0) m-Chloroperbezoic acid (2.81g) was added to a solution of [(N-Boc-piperidin-4- ylmethyl]-(4-methoxyphenylmethyl)sulphide (2g) in dichloromethane (50 ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 16 hours. The reaction mixture was washed with 2M NaOH (20 ml), brine (20 ml) and dried. The solvent was evaporated and the residue was purified on a 50g silica Bond Elut eluting with a solvent gradient (isohexane-50% ethyl acetate/isohexane) to give the title compound, yield 1.75g,

MH" (-Boc) 284.11.

Step 3 Preparation of [(piperidin-4-yl)methyl]}-(4-methoxyphenylmethyl)sulphone hydrochloride

OMe {(N-Boc-piperidin-4-yl)methyl]-(4-methoxyphenylmethyl)sulphone (1.7 5g) was stirred with 4M HCl in dioxane (10 ml) for 30 minutes. The reaction mixture was triturated with diethyl ether and the solid obtained was filtered and dried. Yield 1.42g. MH" 284.

The following compounds were also prepared using a process analogous to Method I

HN OO Rte

I LS

Method J

Preparation of [(piperidin-4-yl)methyl]-(4-methanesulphonylphenylmethyl)sulphone

TEC

N Oo 0 7°

J ~

Step 1 Preparation of [(N-Boc-piperidin-4-yl)methylthioacetate 0]

SAS

>

C15 ©

Potassium thioacetate (1.857 g) was added to a solution of 4-tosyloxymethyl-N-Boc- piperidine (CAS 166815-96-9) (3 g) in DMF (40 ml) and the mixture was heated at 100°C for 4 hours. The reaction mixture was allowed to cool to room temperature and water (5 ml) was added. The reaction mixture was extracted with diethyl ether (3x50 ml). The diethyl ether extracts were washed with saturated aqueous sodium bicarbenate (50 ml), brine (50 ml) and were dried. Removal of the solvent gave an orange oil (2.2 g), MH" 174, NMR (CDCl) : 1.2 (m, 2H), 1.45 (s, 9H), 1.6 (m, 1H), 1.75 (bd, 2H), 2.35 (s, 3H), 2.65 (bt, 2H), 2.8 (d, 2H), 4.1 (m, 2H). This material was used without further purification.

Step 2 Preparation of N-Boc-piperidin-4-ylmethylthiol. (J o N

TY

Sodium borohydride (2.2 g) was added in portions over 10 minutes to a solution of (N-

Boc-piperidin-4-yl)methylthioacetate (2.2 g) in methanol (40 ml) at 0°C. The mixture was allowed to warm to room temperature and was stirred for 2 hours. The reaction mixture was evaporated and the residue was dissolved in water (10 ml), citric acid (2g) was added and the mixture was extracted with dichloromethane (3x20 ml) and dried. Removal of the solvent gave the product as an orange oil, which by NMR contained ~29% of the starting material.

This product was used without further purification.

Step 3 Preparation of [(N-Boc-piperidin-4-yl)methyl}-(4-methanesulphonyl- phenylmethyl)sulphide

SHAS! >ro 52° ! ]

N-Boc-piperidin-4-ylmethylthiol (1.155 g) was added to a suspension of sodium hydride (200 mg of a 60% dispersion in mineral oil) in DMF at 0°C and the mixture was stirred for 30 minutes. 4-Methanesulphonylbenzy! chloride (1.023 g) was added, the reaction mixture was allowed to warm to room temperature and was stirred for 1 hour. The reaction mixture was evaporated to dryness and the residue was dissolved in dichloromethane (30 ml) and washed with water (25 ml) and brine (25 ml) and dried. The solvent was evaporated and the residue was purified on a 50g silica Bond Elut eluting with a solvent gradient (isohexane- 50% ethyl acetate/isohexane). Yield 1g, MH" 300. NMR (CDCl) : 1.2 (m, 2H), 1.45 (5, 9H), 1.5 (m, 1H), 1.8 (m, 2H), 2.35 (4, 2H), 2.65 (bt, 2H), 3.05 (s, 3H), 3.75 (s, 3H), 4.1 (m, 2H), 7.5 (d, 2H), 7.9 ( d, 2H).

Step 4 Preparation of [(N-Boc-piperidin-4-yl)methyl]-(4-methanesulphonyl- phenylmethyl)sulphone.

This was carried out as described in Step 2 of Method I. MH" 376 (- tert-Butyl): NMR . 5 (CDCl) :1.3 (m,2H), 1.4 (s, 9H), 1.9 (m, 2H), 2.2 (m, 1H), 2.7- 2.9 (m, 4H), 3.1 (s, 3H), 4.1 (m, 2H), 4.3 (s, 2H), 7.6 (d, 2H), 8.0 (4, 2H).

Step 5 [(piperidin-4-yl)methyl]-(4-methanesulphonylphenylmethyl)sulphone

This was carried out as described in Step 3 of Method I, MH" 332.

The following compounds were also prepared using a process analogous to Method J. x [wer

Method K 4-(2-[Piperidin-4-yljethylsulphonyl)benzyl methyl sulphone

Qo 0,0

Jes

Step 1 Preparation of 4-(2-[N-Boc-piperidin-4-yljethylsulphonyl)benzyl alcohol 0

S

0 Sh OP

TY

Lithium aluminium hydride (2.823 ml of a 1M solution in THF) was added dropwise to a solution of ethyl 4-(2-[N-Boc-piperidin-4-yl]ethylsulphonyl)benzoate (1.2 g) [prepared according to Method B using ethyl-4-mercaptobenzoate (CAS 28276-32-6) as starting material] in THF (20 ml) at 0°C and the mixture was stirred for 30 minutes. Ethyl acetate (10 ml) was added followed by water (0.1 ml), 2M NaOH (0.1 ml) and water (1 ml) and Celite (2 2). The mixture was stirred for 5 minutes and filtered. The filtrate was evaporated to dryness to give 1.086g, NMR (CDCl;) : 1.0-1.1 (m, 2H), 1.4 (s, 9H), 1.55-1.7 (m, 5H), 2.6 (bt, 2H), 3.1 (m, 2H), 4.1 (m, 2H), 4.8 (s, 2H), 7.6 (d, 2H), 7.9 (d, 2H).

Step 2 Preparation of 4-(2-[N-Boc-piperidin-4-yl]ethylsulphonyl)benzyl alcohol tosylate

Qo

S sot T CL. 0 0 o p-Toluenesulphonyl chloride (541 mg) was added to a solution of 4-(2-[N-Boc- piperidin4-yl]ethylsulphonyl)benzyl alcohol (1.086 g) and triethylamine (0.473 ml) in dichloromethane (30 ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 16 hours. The reaction mixture was washed with water (25 ml), dried and evaporated to dryness. The residue obtained on evaporation of the solvent was passed through a 50g silica Bond Elut column eluting with a solvent gradient (isohexane-50% ethyl acetate/isohexane), yield 765 mg. LC-MS showed this to be a mixture of the required tosylate and the chloro analogue. The mixture was used for the next step.

Step 3 Preparation of 4-(2-[N-Boc-piperidin-4-yl]ethylsulphonyl)benzyl methyl thioether.

Qo soft I Ts

The chloride/tosylate mixture from Step 2 was added to a solution of the sodium salt of methanethiol in DMF at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 16 hours then evaporated to dryness. The residue was dissolved in dichloromethane (20 ml) and washed with water (20 mt) and brine (20 ml) and dried. Evaporation of the solvent gave the product, yield 602 mg, MH" 314.

Step 4 Preparation of 4-(2-[N-Boc-piperidin-4-ylJethylsulphonyl)benzyl methyl sulphone. 220

SRRSY

> i 0) m-Chloroperbenzoic acid (720 mg) was added to a solution of the thioether (Step 3) in dichloromethane (20 ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 16 hours then washed with 2M NaOH (20 ml) and brine (20 ml) and dried. The residue obtained on evaporation of the solvent was passed through a 50g ] silica Bond Elut column eluting with a solvent gradient (isohexane-50% ethyl acetate/isohexane), yield 416 mg, MH" 390 (- tert-Butyl). NMR (CDCl) : 1.0-1.2 (m, 2H), 1.4 (s, 9H), 1.5 (m, 1H), 1.6 (m,2H), 1.7 (m, 2H), 2.6 (bt, 2H), 2.85 (s, 3H), 3.1 (m, 2H), 4.1 (m, 2H), 4.3 (m, 2H), 7.8 (d, 2H), 7.95 (4, 2H).

Step 5 Preparation of the title compound. 4-(2-[N-Boc-piperidin-4-yl]ethylsulphonyl)benzyl methyl sulphone (402 mg) was stirred in 4M HC] in dioxane (10 ml) for 1 hour then diethylether was added and the precipitated solid was filtered and dried, yield (HCl salt) 375 mg. MH" 346.

Method LL 4-(2-[Piperidin-4-yl]ethylsulphonyl)benzamide

A 20

S oaaeW ©

Step 1 Preparation of 4-(2-[N-Boc- piperidin-4-yl]ethylsulphonyl)benzamide

A mixture of ethyl 4-(2-[N-Boc-piperidin-4-yl]ethylsulphonyl)benzoate (0.8 g) [prepared according to Method B using ethyl-4-mercaptobenzoate (CAS 28276-32-6) as starting material] in methanolic ammonia (10 ml of 7M ammonia in methanol) was heated to 50°C to give a clear solution and was allowed to stand at room temperature for 72 hours. The reaction mixture was evaporated to dryness and the residue obtained on evaporation of the solvent was passed through a 50g silica Bond Elut column eluting with a solvent gradient (isohexane-50% ethyl acetate/isohexane), yield 394 mg, MH" 297 (-Boc).

Step 2 Preparation of title compound 4-(2-[N-Boc- piperidin-4-yl]ethylsulphonyl)benzamide (394 mg) was stirred in 4M

HCI in dioxane (10 ml) for 1 hour then diethylether was added and the precipitated solid was filtered and dried, yield (HCI salt) 428 mg. MH" 297.

Method M

Preparation of {4-[(2-piperidin-4-ylethyl)sulfonyl]phenoxy} acetonitrile

Step 1 tert-Butyl 4-(2- {[4-(cyanomethoxy)phenyl}sulfonyl} ethyl)piperidine-1-carboxylate

Bromoacetonitrile (320 mg) was added to a solution of N-tert-butoxycarbonyl-4-[2-(4- hydroxyphenylsulphonyl)ethyl]piperidine (1 g) in acetone (20 ml) containing potassium carbonate (037g ) and the mixture was stirred at room temperature for 16 hours. The reaction mixture was filtered and the filtrate was evaporated to dryness. The residue was dissolved in ethyl acetate (50 ml) and the solution was washed with water (2x50 ml), dried and evaporated to dryness, yield 1.4g. NMR (CDCl): 1.1 (m, 2H), 1.42 (s, 9H), 1.6 (m, 5H), 2.6 (t, 3H), 3.1 (m, 2H), 4.1 (m, 2H), 4.9 (5, 2H), 7.1 (d, 2H), 7.9 (d, 2H).

Step 2 4-(2-{[4-(Cyanomethoxy)phenyl]sulfonyl}ethyl)piperidine tert-Butyl 4-(2- {[4-(cyanomethoxy)phenyl]sulfonyl} ethyl)piperidine-1-carboxylate (1.4g ) was dissolved in dioxane (5 ml) and HCl/dioxane (20 ml of 4M solution) was added.

The mixture was stirred for 1 hour and diethyl ether was added (75 ml) and the oily precpitate obtained was triturated to give {4-[(2-piperidin-4-ylethyl)sulfonyl]phenoxy}acetonitrile hydrochloride, yield 0.9g. MH 309.

Method N

Preparation of 4-(2- {[4-(2-methyl-2H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine.

Step 1 Benzyl 4- {2-[(4-cyanophenyl)sulfonyl]ethyl} piperidine-1-carboxylate.

Benzyl chloroformate (800 mg) was added to a solution of 4-[(2-piperidin-4- ylethyl)sulfonyl]benzonitrile (1.4g, Method B) and triethylamine (1.3g) in dichloromethane (25 ml) at 0°C. The reaction mixture was allowed to warm to room temperature and was stirred for 2 hours, washed with 2M HCl (2x20 m!) and 2M NaOH (2x20 ml) and dried. The residue obtained on removal of the solvent was purified by chromatography on a Bond-Elut column using an eluant gradient of hexane - 50% ethyl acetate/hexane. Yield 1.4g. NMR (CDCL): 0.9 (m, 1H), 1.1 (m, 1H), 1.8 (m, 4H), 2.7 (mm, 2H), 3.1 (m, 2H), 4.2 (m, 2H), 5.1 (s, 2H), 7.3 (m, 5H), 7.9 (d, 2H), 8.05 (d, 2H).

Step 2 Benzyl 4-(2-{[4-(1H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine-1-carboxylate.

The product from step 1 was mixed with sodium azide (220 mg) and ammonium chloride (182 mg) in DMF (25 ml) and heated at 100 °C for 4 hours. The reaction mixture was allowed to cool and the solvent was evaporated. The residue was dissolved in dichloromethane (50 ml) and washed with 2M NaOH (2x20 ml) and dried. Removal of the solvent gave the product, yield 1.9g, M'H 456, which was used directly in the next stage.

Step 3 Preparation of benzyl 4-(2-{ [4-(2-methyl-2H-tetrazol-5-yl)phenyl]sulfonyl}ethyl)- piperidine- 1-carboxylate and benzyl 4-(2- {[4-(1-methyl-1H-tetrazol-5-yl)phenyl]sulfonyl} - ethyl)piperidine-1-carboxylate

Methyl iodide (710 mg) was added to a solution of the product from step 2 (1.9g) in ethanol (25 ml) containing 2M NaOH (5 ml) and the mixture was stirred for 16 hours. A second portion of methyl iodide (710 mg) and 2M NaOH (5 ml) was added and stirring was continued for 72 hours. The reaction mixture was concentrated and water (30 ml) added. The precipitated solid was collected, dried and dissolved in dichloromethane and passed through a

Bond-elut column eluting with 60% ethyl acetate in hexane to give: : 30 Benzyl 4-(2-{[4-(2-methyl-2H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine- 1-carboxylate, yield 800 mg, NMR (CDCl): 1.1(m, 2H), 1.8 (m, 5H), 2.7 (m, 2H), 3.1 (m, 2H), 4.1 (m, 2H), 4.4 (s, 3H), 5.1 (s, 2H), 7.3 (m, 5H), 8.05 (d, 2H), 8.35 (d, 2H). M'H 470.

Benzyl 4-(2- {[4-(1-methyl-1H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine-1-carboxylate, yield 200 mg, NMR (CDCl;): 1.1(m, 2H), 1.8 (m, SH), 2.7 (m, 2H), 3.1 (m, 2H), 4.1 (m, 2H), 4.15 (s, 3H), 5.1 (s, 2H), 7.4 (m, 5H), 7.95 (d, 2H), 8.15 (d, 2H). MH 470. : S Step 4 Preparation of 4-(2-{[4-(2-methyl-2H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine. 20% Palladium hydroxide on charcoal was added to a solution of benzyl 4-(2-{[4-(2- methyl-2H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine-1-carboxylate (700 mg) in a mixture of ethyl acetate (50 ml) and ethanol (150 ml) and the mixture was hydrogenated under a hydrogen filled balloon. The catalyst was filtered and the filtrate evaporated to dryness to give the title compound as a white solid, yield 600 mg. NMR (CDCl): 1.1 (m, 2H), 1.7 (m, 5H), 2.6 (m, 2H), 3.05 (m, 2H), 3.15 (m, 2H), 4.4 (s, 3H), 8.0 (d, 2H), 8.39 (4, 2H).

The corresponding 1-methyl analogue was prepared in an analogous manner starting with benzyl 4-(2-{[4-(1-methyl-1H-tetrazol-5-yl)phenyl]sulfonyl} ethyl)piperidine-1- carboxylate, yield 120 mg.

Method O

Preparation of 4-[(2-piperidin-4-ylethyDsulfonyl]aniline. oO, Be

SARS!

NH,

Step 1 tert-Butyl 4- {2-[(4-aminophenyl)sulfonyl]ethyl}piperidine-1-carboxylate

Nickel (II) acetate tetrahydrate (45 mg) was added to borohydride exchange resin (borohydride on Amberlite® IRA-140 [available from Aldrich]) (3.61 g) in methanol (35 ml) and after the reaction had subsided was allowed to stand for 1 minute. A solution of tert- butyl 4-{2-[(4-nitrophenyl)sulfonyl]ethyl} piperidine-1-carboxylate (717 mg) [prepared according to Method B] in methanol (5 ml) was added and the mixture was stirred at room temperature for 16 hours. The reaction mixture was filtered through Celite® and the resin was washed with methanol (3x10 ml). The combined filtrate and washings were evaporated to dryness and the product was used without further purification. LC/MS, M+H 269 (product -Boc group).

Step 2 Preparation of title compound

The product from step 1 (450mg) was dissolved in 4M HCl in dioxane (10 ml) and allowed to stand for 30 minutes. Diethyl ether (20 ml) was added and a solid was obtained on trituration, yield 507 mg, M+H 269.

Method P

Preparation of N-{4-[(2-piperidin-4-ylethyl)sulfonyljphenyl} methanesulfonamide. oO, 0

S

Saaet!

N PEN

Step 1 Preparation of tert-butyl 4-[2-({4-[(methylsulfonyl)amino]phenyl}thio)ethyl]- piperidine-1-carboxylate.

Methanesulphonyl chloride (0.63 ml) was added to a solution of tert-butyl 4-{2-[(4- aminophenyl)thio]ethyl} piperidine-1-carboxylate (1.61g, Method B) in pyridine (40 ml) at 0°C and allowed to warm to room temperature. The reaction mixture was stirred for 5 hours then evaporated to dryness. The residue was dissolved in dichloromethane (40 ml) washed with water (2x20 ml) and dried. The residue was purified by chromatography on a 50g silica

Bond-elut column using an eluant gradient of hexane-50% ethyl acetate/hexane. Yield 320 mg. M+H 413.

Step 2 Preparation of tert-butyl 4-[2-({4-[(methylsulfonyl)amino]phenyl} sulfonyl)ethyl]- piperidine-1-carboxylate. m-Chloroperbenzoic acid (375 mg) was added to a solution of the product from step 1 (314 mg) in dichloromethane (30 ml) at 0°C and was stirred for 3 hours. The reaction mixture was washed with aqueous sodium bicarbonate (20 ml), brine (20 ml) and dried.

Removal of the solvent gave tert-butyl 4-[2-({4- [(methylsulfonyl)aminc]phenyl}sulfonyl)ethyl]piperidine-1-carboxylate, 330 mg, M+H 347.

Step 3 Preparation of title compound

The tert-butoxycarbonyl group was removed using the procedure described in step 2 of Method O to give the title compound as the hydrochloride salt, M+H 347.

Method Q

Preparation of N-phenyl-N'- {4-[(2-piperidin-4-ylethyl)sulfonyl]phenyl} urea 0, 0 . S fo)

OHO

Phenylisocyanate (86 ul) was added to a solution of tert-butyl 4- {2-[(4- aminophenyl)sulfonyl]ethyl} piperidine-1-carboxylate (300 mg, Method O) in dichloromethane (10 ml) and the mixture was stirred for 16 hours. A further equivalent of phenylisocyanate was added and stirring continued for 24 hours. The reaction mixture was poured onto a 20g silica Bond-elut column and eluted with a solvent gradient of hexane-70% ethyl acetate/hexane. M'H 388 (M- Boc).

The tert-butoxycarbonyl group was removed using the procedure described in step 2 of Method O to give the title compound as the hydrochloride salt, yield 124 mg, M+H 388.

Method R

Preparation of (3R)-3-(3,5-difluorophenyl)-3-[4-(methylsulfonyl)phenyl]propanoic acid of

WJ oO — ) ©

F

To a stirred solution of (4S,5R)-1- {(3R)-3-(3,5-difluorophenyl)-3-[4- (methylsulfonyl)phenyljpropanoyl}-3,4-dimethyl-5-phenylimidazolidin-2-one (7.5g) (prepared according to Method A, step 1, using 3,5-difluorphenylmagnesium bromide) in

THF (300ml) was added a solution of lithium hydroxide monohydrate(2.0g) in water (30ml).

After stirring for 16 hours at 20 — 25°C, the solution was evaporated at reduced pressure and the residue partitioned between water (200ml) and dichloromethane (200ml). The aqueous layer was separated and washed again with dichloromethane, then acidified to pH 2 with 2N

HCl and the precipitate extracted into ethyl acetate (200ml) which was dried over magnesium sulphate and evaporated to give 4.8 gm pale cream solid (96% yield), NMR: 3.10 (m, ZH), 3.15 (s, 3H), 4.60 (dd, 1H), 7.02 (t, 1H), 7.18 (4, 2H), 7.67 (d, 2H), 7.82 (d, 2H).

Method S

Preparation of (3R)-3-(3,5-difluorophenyl)-N-methoxy-N-methyl-3-[4- (methylsulfonyl)phenyl]propanamide \ 2 0=S§

Oo—

N

5

F

To a stirred mixture of (3R)-3-(3,5-difluorophenyl)-3-[4-(methylsulfonyl)phenyl}- propanoic acid (4.8g), N,O-dimethyl hydroxylamine hydrochloride (1.5g) and HATU (1.5g) in dichloromethane (200 ml) was added DIPEA (10 ml) and stirring was continued for 16 hours at 20 — 25°C; water (100 ml) was added and the organic layer separated, then washed ' successively with 1M HCI, 1M NaOH and water. The solution was dried MgSO,), evaporated and the residue purified by chromatography on silica, eluting with ethyl acetate to give 4.7g (gum) 87% yield. NMR (CDCL): 3.04 (s, 3H), 3.13 (s, 3H), 3.18 (d, 2H), 3.65 (5, 3H), 4.76 (t, 1H), 6.67 (t, 1H), 6.78 (d, 2H), 7.44 (d, 2H), 7.89 (d, 2H).

Method T

Preparation of (4R)-4-(3,5-difluorophenyl)-4-[4-(methylsulfonyl)phenyl]butan-2-one \ 0] o=¢’ 5H

F

To a stirred solution of (3R)-3-(3,5-difluorophenyl)-N-methoxy-N-methyl-3-[4- (methylsulfonyl)phenyl]propanamide (4.7g) in dry THF (50 ml)under argon, cooled to —20°C,

was added 12.0ml 3M methyl magnesium bromide (12 ml of a 3M solution in ether). The reaction was stirred for a further 1 hour at 0°C, then 50ml IM HCl was cautiously added and the mixture extracted with ethyl acetate, dried and evaporated to give 4.1gm (gum) 99% yield.

NMR (CDCl): 2.16 (s, 3H), 3.043 (s, 3H), 3.21 (d, 2H), 4.69 (t, 1H), 6.67 (t, 1H), 6.77 (d, 2H), 7.41(d, 2H), 7.89 (d, 2H).

Method U (S) N-(3-Amino-3-phenylpropyl)-4-[2-(4-methanesulphonylphenylsulphonyl)ethyl]piperidine

AO

AO

. I ~~

Sodium triacetoxyborohydride (1.6g) was added to a mixture of (S) 3-phenyl-3-(tert- butoxycarbonylamino)propionaldehyde (1.23g) and 4-[2-(4-methanesulphonylphenyl- sulphonyl)ethyl]piperidine hydrochloride (1.215g) (Method B) in dichloromethane (50ml) and the mixture was stirred for 16 hours. The reaction mixture was washed successively with 2M sodium hydroxide (15ml), water (15ml) and brine (15ml) and dried. The dichloromethane solution was stirred with PS-NCO (isocyanate resin, 1.5g) for 16 hours and filtered. The filtrate was chromatographed on a 50g silica Bond Elut column eluting with ethyl acetate to give the Boc protected title compound as a white solid, yield 1.595g, MH" 565.

The Boc protected compound (1.59g) was dissolved in 4M HCl/dioxane (10ml)-and allowed to stand at room temperature for 1 hour. The reaction mixture was evaporated to dryness, redissolved in 2M sodium hydroxide (10ml) and extracted with dichloromethane (2x20ml) and dried. Removal of the solvent gave the title compound, yield 0.56g, MH" 465. (8S) 3-phenyl-3-(tert-butoxycarbonylamino)propionaldehyde

AK oy oy

Lithium aluminium hydride (19 mi of IM solution in THF) was added to a solution of (S) 3-phenyl-3-(tert-butoxycarbonylamino)propionic acid (5.018) in THF (50ml) at 0°C. The reaction mixture was stirred for 1 hour and ethyl acetate (20ml) was added followed by water (0.5ml), 6M sodium hydroxide (0.5ml) and water (5ml). The mixture was filtered through Celite and evaporated to dryness to give (S) 3-phenyl-3-(tert-butoxycarbonylamino)propanol, yield 2.89g. This material was dissolved in dichloromethane (40ml) and Dess Martin periodinane (2.12g) was added. The reaction mixture was stirred for 1 hour then washed with 2M sodium hydroxide (2x20ml) and brine (10ml) and dried. The dichloromethane solution was concentrated to a volume of about 20ml and used directly in the next stage.

Example 19

The ability of compounds to inhibit the binding of RANTES was assessed by an in vitro radioligand binding assay. Membranes were prepared from Chinese hamster ovary cells - which expressed the recombinant human CCRS5 receptor. These membranes were incubated with 0.1nM iodinated RANTES, scintillation proximity beads and various concentrations of the compounds of the invention in 96-well plates. The amount of iodinated RANTES bound to the receptor was determined by scintillation counting. Competition curves were obtained for compounds and the concentration of compound which displaced 50% of bound iodinated © RANTES was calculated (ICso). Certain compounds of formula (I) have an ICs of less than 50uM.

Example 20

The ability of compounds to inhibit the binding of MIP-1a was assessed by an in vitro radioligand binding assay. Membranes were prepared from Chinese hamster ovary cells which expressed the recombinant human CCRS5 receptor. These membranes were incubated with 0.1nM iodinated MIP-1a. , scintillation proximity beads and various concentrations of the compounds of the invention in 96-well plates. The amount of iodinated MIP-1a bound to. the receptor was determined by scintillation counting. Competition curves were obtained for compounds and the concentration of compound which displaced 50% of bound iodinated

MIP-10a was calculated (ICs). Certain compounds of formula (I) have an ICs of less than 50pM.

Results from this test for certain compounds of the invention are presented in Table

XV. In Table XV the results are presented as Pic50 values. A Pic50 value is the negative log (to base 10) of the ICs result, so an IC50 of 1uM (that is 1 x 10M) gives a Pic50 of 6. Ifa compound was tested more than once then the data below is an average of the probative tests results.

TABLE XV

LE CE

EE LA LE a LE LN

EE EN LN

RA LA LA cE LE UC

Ec LL

Scheme 1

To prepare compounds of the invention, for example wherein R' is aryl or C-linked piperidine. :

N° . Rr! (0) 1 1 ! OY ii R 0) iii R OH . 2 —_— 2 — ~Y —

R R07 RF Or TT iv v R! 0 2X rR? — .

A an NY ~~ R

R? i Wittig reaction (eg LHDMS, triethylphosphonoacetate) ii Catalytic hydrogenation (eg Hz, 10% Pd/C) ili Reduction (eg LAH) iv Oxidation (eg Dess-Martin oxidation)

Silas

N v reductive ammination with (eg using sodium triacetoxyborohydride)

Scheme 2

To prepare compounds of the invention, for example wherein R'is aryl or C-linked piperidine.

R 0 .

NY i R 0 R! o)

R® OH RZ R / ii xt Tere 1 A

RR R

1 Base hydrolysis (eg LiOH, MeOH/H20) ii MeMgCl, R°MgBr, Et;,0

Oikala ii reductive amination N in presence of titanium tetra- isopropoxide (eg using sodium triacetoxyborohydride)

Scheme 3

To prepare compounds of the invention, for example wherein R! is aryl, heteroaryl, heterocyclyl or NRP>C(O)R™. 1

NS rR! L , he

R te

A xt Ter!

N

- xt Tere 1 A

NT

R? in which L is an activated group, such as halogen, mesylate, tosylate or triflate.

Scheme 4

To prepare compounds of the invention, for example wherein R! is aryl, heteroaryl, heterocyclyl or NR>C(O)R™.

AS of ° Dial 1 ) _— YY ___. R N

Ro © RRL YY

R 0]

CY hd ; A

R NO

2

R in which L! is a halogen, an activated ester or a complex formed with a carbodiimide.

Scheme 5

To prepare compounds of the invention, for example wherein R! is NRP>C(O)R'™. 0) 0 0 0 1 he i hd xt Tere ~NY a N A

R R i

R'__O 7 ikala ~ Flute

N N ii HN N 1 reductive amination (if R? is H can use sodium triacetoxyborohydride; if R’ is alkyl can use titanium tetra-isopropoxide and sodium triacetoxyborohydride) 1 Deprotection (eg TFA) ii amide bond formation (eg acid chloride, active ester or carbodiimide mediated)

Scheme 5

To prepare compounds of the invention, for example wherein R' is piperazine.

R? . 2 . ~ Y i NY i A xt Tere

OH OH —™ 4, [2 —= oN

R? jii

A xt Tere

P< 4 iv Cl N @ Skala NO

R

\ vi \ v

RJ

NN xt Tire NY xT Tr (UN N 0 vi ow N 0 1 Conversion of an OH to a leaving group (eg tosyl chloride (L? is Tosylate) or mesyl chloride (1.2 is Mesylate))

Skala

N ii displacement reaction with (eg in presence of triethylamine) iii Mesyl chloride, DCM 0°C iv Displacement reaction with mono-protected piperazine (P is a protecting group) wv Displacement reaction with R substituted piperazine vi Deprotection (TFA for Boc, hydrogenation for Cbz) vii Depending on R, acylation, sulphonylation, alkylation, reductive amination

Scheme 6

To prepare compounds of the invention, for example wherein R! is aryl or piperidine. o. [/

RNA o ! N

SAN i NIN } ; NY ii R N oe TT SNOT

R° O yo / iii

R H 1

NY v NT

R”” 0 ~~ rR? gs ik xt Trt 1 A

NT

RZ oy

Si activation of acid group and coupling with chiral auxiliary (eg SOC; , ) ii 1,4-addition of organocuprate (eg R®*MgBr, Cu(I)I, TMEDA, di-butylboron triflate) iii reduction (eg lithium aluminium hydride) iv Dibal v Oxidation (eg Dess-Martin reagent) wi reductive amination (eg with sodium triacetoxyborohydride)

Scheme 7

To prepare compounds of the invention.

A HOH .

R! N | A nL

NY — R N

Rr’ YY

Rr \, xt Imre xt Te R* 1 A

NT

RZ

1 activation via halide, tosylate, mesylate, triflate i base catalysed displacement

Claims (22)

1. A compound of formula (I): y 3 = (CHp),—X—(CHp),—R* (1 wherein: A is absent or is (CHa); R' is C14 alkyl, C(O)NR'®R", C(O),R'?, NR">C(O)R", NR"*C(O)NR'°R"7, NR'®C(0),R", heterocyclyl, aryl or heteroaryl; RR", R', R'® and R'® are hydrogen or C; alkyl; R!! RY? R* R' and R" are C,.3 alkyl (optionally substituted by halo, hydroxy, Ci alkoxy, C;.¢ haloalkoxy, Cs. cycloalkyl (optionally substituted by halo), Cs. cycloalkenyl, S(Cy4 alkyl), S(O)(C1.4 alkyl), S(0)2(C1.4 alkyl), heteroaryl, aryl, heteroaryloxy or aryloxy), aryl, heteroaryl, Cs.7 cycloalkyl (optionally substituted by halo or C4 alkyl), C47 cycloalkyl fused to a phenyl ring, Cs. cycloalkenyl, or, heterocyclyl (itself optionally substituted by oxo, C(O)(Ci. alkyl), S(O)(Ci.¢ alkyl), halo or C4 alkyl); or RY, RZ, rR! and R' can also be hydrogen; or R' and RY, and/or R'® and R'” may join to form a 4-, 5- or 6-membered ring which optionally includes a nitrogen, oxygen or sulphur atom, said ring being optionally substituted by C6 alkyl, S(O)(C\. alkyl) or C(O)(Ci.6 alkyl); R? is C1. alkyl, phenyl, heteroaryl or Cs.7 cycloalkyl; R? is H or C4 alkyl; R* is aryl, heteroaryl, C,.¢ alkyl or C3.7 cycloalkyl; X is O or S(O); m and n are, independently, 0, 1, 2 or 3, provided m +n is 1 or more; aryl, phenyl and heteroaryl moieties are independently optionally substituted by one or more of halo, cyano, nitro, hydroxy, OC(O)NR*R*, NR?R?, NR*C(O)R”, NR¥C(O)NR?'R%, S(0),NR®R*, NR*'S(0),R*2, C(O)NR*R*, COR, NR¥CO,R®, S(0);R¥, 0S(0),R*, C,. alkyl (optionally mono-substituted by S(0):R*® or C(O)NR®'R*?), C,.6 alkenyl, Cy.¢ alkynyl, Cs.19 cycloalkyl, Cy.¢ haloalkyl,

C1.6 alkoxy(C,.¢)alkyl, C,.¢ alkoxy (optionally mono-substituted by CO,R>?, C(O)NR*R**, cyano, heteroaryl or C(O)NHS(O);R*¢), NHC(O)NHR”’, Cy.

haloalkoxy, phenyl, phenyl(C;.4)alkyl, phenoxy, phenylthio, phenylS(O), phenylS(O)z,

phenyl(C;4)alkoxy, heteroaryl, heteroaryl(Ci4)alkyl, heteroaryloxy or heteroaryl(C.

a)alkoxy; wherein any of the immediately foregoing phenyl and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(Ci.4 alkyl), S(O)(Ci.4 alkyl), - S(0)2(C14 alkyl), S(0)NHz, S(0)NH(C).4 alkyl), S(0)N(C,.4 alkyl), cyano, C4 alkyl, C;.4 alkoxy, C(O)NH,, C(O)NH(C,. alkyl), C(O)N(C, 4 alkyl), COH, CO2(C,.

4 alkyl), NHC(O)(C, 4 alkyl), NHS(O)(C4 alkyl), CF; or OCF;;

unless otherwise stated heterocyclyl is optionally substituted by Ci. alkyl [optionally substituted by phenyl {which itself optionally substituted by halo, C14 alkyl, C14 alkoxy, cyano, nitro, CFs, OCF3, (C14 alkyl)C(O)NH, S(0),NH,, C,.4 alkylthio, S(O)(C)-4 alkyl) or S(O)2(Ci.4 alkyD)} or heteroaryl {which itself optionally substituted by halo, C1 alkyl, C;4 alkoxy, cyano, nitro, CFs, (C14 alkyDDC(O)NH, S(O) NH, Ci4 alkylthio, S(O)(C14 alkyl) or S(O),(Ci4 alkyl)}], phenyl {optionally substituted by halo, C;.4 alkyl, C,4 alkoxy, cyano, nitro, CF3, OCF3, (Cy alkyl)C(O)NH, S(O),NH.,

C14 alkylthio, S(O)(C14 alkyl) or S(0)2(C4 alkyl)}, heteroaryl {optionally substituted by halo, C4 alkyl, Cy4 alkoxy, cyano, nitro, CF3, (C1.4 alkyl)C(O)NH, S(O)NH;, C14 alkylthio, S(O)(C1 alkyl) or S(0)x(C1.4 alkyl)}, S(O): NR*R*, C(O)R™, C(0)x(C1. alkyl) (such as tert-butoxycarbonyl), C(O).(phenyl(C,.; alkyl)) (such as benzyloxycarbonyl), C(O)NHR®, S(O),R*, NHS(O);NHR*, NHC(O)R™,

NHC(O)NHR* or NHS(O);R*, provided none of these last four substituents is linked to a ring nitrogen;

k, 1, p and q are, independently, 0, 1 or 2; R® RZ R* R* RY R® R¥,R® RY R¥, R’ and R* are, independently, hydrogen or C,.¢ alkyl;

RZ RZ, R® R®, R®, RZ, R* R¥, R*, R¥ RY, R*2 R® R™ R* R% RY, R® RY, RY, R%, R®, R%, R* and RY are, independently, C;.¢ alkyl (optionally substituted by halo, hydroxy, C.s alkoxy, Ci. haloalkoxy, Cs.s cycloalkyl, Cs. cycloalkenyl, S(Ci4

} alkyl), S(O)(C1.4 alkyl), S(O)2(Ci4 alkyl), heteroaryl, phenyl, heteroaryloxy or phenyloxy), Cs. cycloalkyl, phenyl or heteroaryl; wherein any of the immediately foregoing phenyl! and heteroaryl moieties are optionally substituted with halo, hydroxy, nitro, S(Ci.4 alkyl), S(O)(C1.4 alkyl), S(0)2(Ci4 alkyl), S(0):NH,, S(0);NH(C 4 alkyl), S(0)?N(C,.4 alkyl),, cyano, C,4 alkyl, C4 alkoxy, C(O)NH,

C(O)NH(C,.4 alkyl), C(O)N(Ci.4 alkyl), COzH, CO(C,4 alkyl), NHC(O)(Ci.4 alkyl), NHS(0)2(C) alkyl), C(O)(C14 alkyl), CFs or OCF; rR? RB RY, R® RP R¥ RY, RY, RY, RZ RY, B® R* RY RS, RR and RY may additionally be hydrogen; or a pharmaceutically acceptable salt thereof or a solvate thereof.

2. A compound as claimed in claim 1 wherein R' is NHC(O)R™, phenyl or heterocyclyl, wherein R' is as defined in claim 1, and phenyl and heterocyclyl are optionally substituted as described in claim 1.

3. A compound as claimed in claim 1 or 2 wherein R? is phenyl or heteroaryl, either of which is optionally substituted by halo, C;4 alkyl, C,4 alkoxy, S(O)x(Ci4 alkyl), nitro, cyano or CF3; whereinn is 0, 1 or 2.

4. A compound as claimed in claim 1, 2 or 3 wherein R3 is hydrogen.

5. A compound as claimed in claim 1, 2, 3 or 4 wherein R'is phenyl optionally substituted by one or more of halo, hydroxy, nitro, S(C1. alkyl), S(O)(C,. alkyl), S(0)(Ci.6 alkyl), S(O).NH;, S(O),NH(C1.s alkyl), S(0)N(C1.6 alkyl), cyano, Ci.6 alkyl, C1. alkoxy, CH;S(0)2(C..¢ alkyl), OS(0)2(C,.¢ alkyl), OCH;heteroaryl, OCH,CO,H, OCH,C0,(C;.¢ alkyl), OCH,C(O)NH,, OCH,C(O)NH(C,.¢ alkyl), OCH,CN, NH,, NH(C, alkyl), N(Cy atkyl)2, C(O)NH,, C(O)NH(C,. alkyl), C(O)N(Ci1. alkyl)z, CO2H, CO5(C1.6 alkyl), NHC(O)(Ci.5 alkyl), NHC(OYO(Ci.6 alkyl), NHS(O)2(C,.¢ alkyl), CFs, CHF, CHzF, CH,CF3, OCF3, heteroaryl or heteroaryl(Ci.4 alkyl); wherein the foregoing heteroaryl groups are optionally substituted by halo, hydroxy, nitro, S(C,4 alkyl), S(O)(Ci.4 alkyl), S(0)2(C,4 alkyl), S(0);NHz, S(O),NH(C, 4 alkyl), S(0)N(C.4 alkyl), cyano, C4 alkyl, C,4 alkoxy, C(O)NH;, C(O)NH(C 4 alkyl), C(O)N(Ci alkyl), COzH, COx(C,4 alkyl), NHC(O)(C1.4 alkyl), NHS(O)2(C14 alkyl), CF3 or OCFs.

6. A compound as claimed in claim 1, 2, 3, 4 or 5 wherein A is absent.

7. A compound as claimed in any one of the preceding claims wherein n is 2.

8. A compound as claimed in any one of the preceding claims wherein m is 0.

9. A compound as claimed in any one of the preceding claims wherein X is S(O).

10. A process for preparing of a compound as claimed in claim 1 comprising:

a. to prepare a compound wherein R? is hydrogen, coupling a compound of formula (IT): HN A (CH,),—X—(CH,),—R* (I) wherein RY, m, n, A and X are as defined in claim 1, with a compound of formula avy: R' H JA (IV) R® 0) wherein R' and R? are as defined in claim 1, in the presence of NaBH(OACc); (wherein Ac is C(O)CH3) in a suitable solvent at room temperature;

b. to prepare a compound wherein R? is hydrogen, coupling a compound of formula (III): HN A (CH,),—X—(CH,),—R* (Ill) wherein R*, m, n, A and X are as defined in claim 1, with a compound of formula (V): R' A R L Vv) wherein R' and R? are as defined in claim 1 and Lis a leaving group, in the presence of a base, in a suitable solvent at a temperature from 60°C to the boiling point of the solvent.

11. A pharmaceutical composition which comprises a compound as claimed in claim], or a pharmaceutically acceptable salt thereof or solvate thereof, and a pharmaceutically acceptable adjuvant, diluent or carrier.

vv A, PCT/SE2003/002008 ® 106

12. A compound as claimed in claim 1, or a pharmaceutically acceptable salt thereof or solvate thereof, for use as a medicament.

13. Use of a compound as claimed in claim 1, or a pharmaceutically acceptable salt thereof or solvate thereof, in the manufacture of a medicament for use in therapy.

14. Use of a compound as claimed in claim 1, or a pharmaceutically acceptable salt thereof or solvate thereof in the manufacture of a medicament for treating a CCRS mediated disease state.

15. A substance or composition for use in a method of treatment, said substance or composition comprising a compound as claimed in claim 1, ora pharmaceutically acceptable salt thereof or solvate thereof, and said method comprising administering said substance or composition.

16. A substance or composition for use in a method of treating a CCR5 mediated disease state, said substance or composition comprising a compound as claimed in claim 1, or a pharmaceutically acceptable salt thereof or solvate thereof, and said method comprising administering an effective amount of said substance or composition to a patient in need of such treatment.

17. A compound according to claim 1, or claim 12, substantially as herein describe and illustrated.

18. A process according to claim 10, substantially as herein described and illustrated.

19. A composition according to claim 11, substantially as herein described and illustrated. AMENDED SHEET yA i. PCT/SE2003/002008 ® 107

20. A substance or composition for use in a method of treatment according to any one of claims 12, or 15, or 16, substantially as herein described and illustrated.

21. Use according to claim 13, or claim 14, substantially as herein described and illustrated.

22. A new compound; a new process for preparing a compound; a new composition; a substance or composition for a new use in a method of treatment; or a new use of a compound as claimed in claim 1, or a pharmaceutically acceptable salt or solvate thereof, substantially as herein describe. AMENDED SHEET