ZA200409490B

ZA200409490B - Compositions and methods for treating diabetes

Info

Publication number: ZA200409490B
Application number: ZA200409490A
Authority: ZA
Inventors: Stephen J Brand; Antonio Cruz
Original assignee: Waratah Pharmaceueticals Inc
Priority date: 2002-06-07
Filing date: 2004-11-24
Publication date: 2006-04-26

Description

COMPOSITIONS AND METHODS FOR TREATING DIABETES

Field of the Invention

The invention relates to methods and compositions for treating a diabetic subject with islet neogenesis therapy (I.N.T.™) and an agent for immunosuppression, and with a gastrin/CCK ligand receptor and an agent for immunosuppression.

Background of the Invention

About 800,000 people in the United States population suffer from insulin deficiency diabetes (also known as juvenile or type I diabetes), and about 30,000 new cases arise each year. Further, an extremely large and rapidly increasing number of patients have forms of type ll diabetes (also called adult onset or insulin-resistance diabetes), in this population at a level of epidemic proportions that cause pancreatic exhaustion and insulin insufficiency.

The abnormally high blood glucose (hyperglycemia) that characterizes diabetes, if left untreated, results in a variety of pathological conditions, for example, non-healing peripheral vascular ulcers, retinal damage leading to blindness, and kidney failure. Diabetes of both types I and II are treated with insulin injection in response to blood glucose levels determined by patient glucose self-monitoring, although not all type II patients progress to requiring insulin therapy. Typically, multiple doses of insulin are delivered by the patient per day. Severe pathological consequences of diabetes are correlated with less rigorous control of blood glucose level.

Summary of Embodiments

In one aspect, an embodiment of the invention is a method for manufacture of a medicament for use in treating a diabetic subject, the method comprising administering to the subject a composition for islet neogenesis therapy and an agent for suppressing an immune response. The method further uses a composition for islet neogenesis therapy that . 30 comprises an EGF receptor ligand. The method further uses a composition for islet neogenesis therapy that comprises at least two agents for suppressing an immune response.

The composition of the method for islet neogenesis therapy comprises a gastrin/cholecystekinin (CCK) receptor ligand. The EGF receptor ligand is a recombinant . human EGF, for example, the EGF receptor ligand is EGF5SIN. The gastrin/CCK receptor ligand is human gastrin17. In related embodiments, the agent for suppressing immune response is a drug, for example, the agent for suppressing immune response is at least one selected from of the group consisting of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6- mercaptopurine; FK506 (Tacrolimus); 15-deoxyspergualin; an FTY 720; a mitoxantrone; a 2- amino-1,3-propanediol; a 2-amino-2[2-(4-octylphenyl)ethyl]propane-1,3-diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; and a demethimmunomycin. The rapamycin is

Everolimus or Sirolimus. The corticosteroid is dexamethasone. The agent for suppressing immune response is a protein, for example, the protein comprises an amino acid sequence of an antibody. Exemplary antibodies for suppressing immune response can be at least one selected from the group consisting of: hul 124; BTI-322; allotrap-HLA-B270; OKT4A; Enlimomab; ABX-CBL; OKT3; ATGAM,; basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247; Medi-500; Medi-507; Alefacept; efalizumab; infliximab; in an alternative embodiment, the protein is a cytokine or growth factor such as GFAP, S100B, or an interferon.

According to these methods, the islet neogenesis therapy composition and the agent for suppressing immune response are formulated for sequential administration. One example comprises formulating the islet neogenesis therapy composition and the agent for suppressing immune response for sequential administration, and fpr allowing a period of time of at least one day between administering the agent and administering the composition. Alternatively, the period is at least one week, or at least six weeks between administering the agent and administering the composition.

In the above embodiments, at least one of the islet neogenesis therapy composition . and the agent for suppressing immune response is formulated for systemic administration.

Alternatively, at least one of the islet neogenesis therapy composition and the agent for : suppressing immune response is ormulated for bolus administration. For example,

at least one of the islet neogenesis therapy composition and the agent for suppressing immune response is formulated for administration by a route selected from the group ] consisting of intravenous, subcutaneous, intraperitoneal, and intramuscular. In certain embodiments, the agent for suppressing immune response is formulated for oral administration. In certain embodiments, the agent for suppressing immune response is at least one selected from the group of Sirolimus, Tacrolimus, Everolimus, ISAtx247, and daclizumab.

In most cases, the subject for which the use is formulated is a diabetic mammal, for example, the subject is a human. Alternatively, the subject can be an experimental animal, for example, an NOD mouse.

In another aspect, an embodiment of the invention is a method for manufacture of a medicament for use in treating a diabetic subject, the method comprising formulating for the subject a composition for islet neogenesis therapy consisting of at least one of an EGF receptor ligand and a gastrin/CCK receptor ligand, and administering at least one immunosuppressing agent. In a related embodiment, the method involves further formulating the gastrin/CCK receptor ligand and the at least one immunosuppressing agent in the absence of the EGF receptor ligand. In one embodiment of the method, prior to further administering the gastrin/CCK receptor ligand and the at least one immunosuppressing agent in the absence of the EGF receptor ligand, the method further includes a period of no administration of any of the agent or the composition. The diabetic subject for example has recent onset diabetes. Further, following the period of no administration, the composition and the agent are formulated for sequential administration. In certain embodiments, each of the EGF receptor ligand, the gastrin/CCK receptor ligand, and the immunosuppressing agent are formulated in an effective dose. The composition or agent further is some embodiments is formulated with a pharmaceutically acceptable buffer.

Yet another feature of the invention is a method of manufacture of a medicament for . use in treating a diabetic subject, the method comprising administering to the subject a composition for islet neogenesis therapy consisting of at least one of EGF51IN and gastrinl7, : and administering at least one immunosuppressing agent. The at least one agent is formulated to be an effective dose of each of Tacrolimus and Sirolimus. Alternatively, the method further involves formulating an effective dose of at least one of EGFSIN and gastrin 17, and formulating an effective dose of at least one of Tacrolimus, Everolimus, daclizumab, . [SAtx247, and Sirolimus. Altematively, the method comprising formulating for use with the subject a gastrin/CCK receptor ligand and an agent for suppressing an immune response. For example, the gastrin/CCK receptor ligand is gastrin17. The agent for suppressing immune response is a drug, for example, the agent for suppressing immune response is selected from at least one of the group consisting of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6- mercaptopurine; an FK506; an ISAtx247; a 15-deoxyspergualin; an FTY 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4-octylphenyl)ethyl]propane-1,3- diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; and a demethimmunomycin.

For example, the rapamycin is Sirolimus or Everolimus. For example, the corticosteroid is dexamethasone.

Alternatively in embodiments of this method, the agent for suppressing immune response is a protein, for example, the protein comprises an amino acid sequence of an antibody. For example, the agent for suppressing immune response is at least one selected from the group consisting of: hul 124; BTI-322; allotrap-HLA-B270; OKT4A; Enlimomab;

ABX-CBL; OKT3; ATGAM; basiliximab; daclizumab; antithymocyte immunoglobulin;

ISAtx247; Medi-500; Medi-507; Alefacept; efalizumab; and infliximab. The method involves formulating the gastrin/CCK receptor ligand and the agent for suppressing immune response administered sequentially. Further, the gastrin/CCK receptor ligand is administered as a bolus. For example, administering the receptor ligand is further using a route selected from the group consisting of intravenous, subcutaneous, intraperitoneal, and intramuscular.

Administering the agent for suppressing immune response is using a route selected from the group consisting of an oral, systemic, intravenous, subcutaneous, intraperitoneal, and intramuscular routes of delivery. In one embodiment of the method, the agent for . suppressing immune response is selected from at least one of Tacrolimus, ISAtx247,

Everolimus, Sirolimus, and daclizumab. . In any of the embodiments of the methods herein, the invention additionally involves measuring a physiological parameter in the subject, for example, measuring level of: fasting blood glucose; pancreatic insulin content; pancreatic § cell content; and plasma insulin C peptide. The subject is a mammal, for example, the subject is a diabetic mammal, for . example, the subject is a diabetic mammal with recent onset diabetes, for example, the subject is a human. 5 The invention also features a method of treating a diabetic subject, the method comprising administering to the subject a composition comprising an effective dose of gastrinl7 and an effective dose of at least one immunosuppressing agent. At least one of the immunosuppressing agents is Tacrolimus or Sirolimus. The method can further involve administering to a subject an effective dose of ISAtx247 or daclizumab. The administered composition further includes a pharmaceutically acceptable buffer.

Also featured is a pharmaceutical composition comprising an agent for suppressing an immune response and at least one of an EGF receptor ligand and a gastrin/CCK receptor ligand.the gastrin/CCK receptor ligand is a gastrin. For example, the gastrin/CCK receptor ligand is a gastrinl7, and the gastrinl7 is gastrin! 7Met15 or gastrinl7Leul5.

Further, the EGF receptor ligand is EGF, for example, the EGF is recombinant human

EGFSIN. Further, the agent for suppressing immune response is a drug. For example, the agent for suppressing immune response is at least one selected from of the group consisting of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6-mercaptopurine; FK506 (Tacrolimus); 15- deoxyspergualin; an FTY 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4- octylphenyl)ethyl]propane-1,3-diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; and a demethimmunomycin. Alternatively, the agent for suppressing immune response is a protein. For example, the agent for suppressing immune response is a portion of an antibody, for example, the agent is at least one selected from the group consisting of: hul 124; BTI-322; allotrap-HLA-B270; OKT4A; Enlimomab; ABX-CBL; OKT3; ATGAM; basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247; Medi-500; Medi-507; ) Alefacept; efalizumab; infliximab; and an interferon.

Another aspect is a kit for treatment of a diabetic subject, comprising a composition for islet neogenesis therapy, an immunosuppressive agent, and a container.

The kit includes at least one dose of any one or more of the compositions herein. The kit can further include instructions for use. . Also featured is a method of manufacture of a medicament for use in treating a diabetic subject, the method comprising manufacture of a composition comprising an effective dose of gastrinl7 and an effective dose of at least one immunosuppressing agent.

For example, a method of manufacture of a medicament for use in treating a diabetic subject, such that the medicament has an effective dose of gastrin17 and an effective dose of each of

Tacrolimus and Sirolimus. Also featured is use of an effective dose of each of gastrinl7 and at least one immunosuppressing agent in manufacture of a composition for treating a diabetic subject. Also featured is use of an effective dose of gastrinl7 and an effective dose of each of Tacrolimus and Sirolimus in manufacture of a medicament for treating a diabetic subject.

Also featured is use of an immunosuppressing agent and at least one of an EGF receptor ligand and a gastrin/CCK receptor ligand for the manufacture of a medicament for treating a diabetic subject.

Alternative diabetes therapies to glucose monitoring and insulin injection have previously been limited by immune rejection of insulin and the B-cells that produce insulin.

Administration of an agent for immune suppression with a composition for islet neogenesis therapy (I.N.T. ™. U.S. patent numbers 5,885,956 and 6,288,301) would be highly advantageous for treatment of diabetes.

A feature of the present invention is a method of treating a diabetic subject by administering to the subject a composition for islet neogencsis therapy (IN.T.™ and an agent for suppressing immune response. The agent can be a protein, for example, all ora portion of an antibody. Alternatively, the agent can be a drug, which as used herein means a low molecular weight compound, having a molecular weight of less than about 5,000 daltons, less than about 2,000 daltons, or less than about 1,000 daltons. The composition for islet neogenesis therapy comprises an EGF receptor ligand, for example, the EGF receptor ligand . is a recombinant human EGF. For example, the EGF receptor ligand is EGF51IN. Further, the composition for islet neogenesis therapy comprises a gastrin/cholecystekinin (CCK) ) receptor ligand. For example, the gastrin/CCK receptor ligand is human gastrinl7 having a leu at position 15.

The agent for suppressing immune response can be at least one of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine (cyclosporin); a . cyclophosphamide; a methotrexate; a 6-mercaptopurine; a FK506; 15-deoxyspergualin; an

FTY 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4- octylphenyl)ethyl}propane-1,3-diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; or a demethimmunomycin. The term “a rapamycin” means any related chemical entity or derivative that substantially shares the rapamycin pharmacore and shares an immunosuppressive activity. The terms “corticosteroid” means any related chemical entity that substantially shares the immunosuppressive activity. For example, the term rapamycin includes Sirolimus (which is rapamycin) and SDZ-RAD, also known as Everolimus. An example of a corticosteroid is dexamethasone.

Alternatively, the agent for suppressing immune response that is the protein comprises an amino acid sequence of an antibody. For example, the agent is at least one of: hul 124; BTI-322; allotrap-HLA-B270; OKT4A; Enlimomab; ABX-CBL; OKT3; ATGAM,; basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247; Medi-500; Medi-507;

Alefacept; efalizumab; infliximab. Yet another alternative is that the agent for suppressing immune response is a protein that is not an antibody, for example, is Glial fibrillary acidic protein (GFAP), or is another glial protein, S100f (Selinfreund, R. et al. J Cell Biol. 1990, 111: 2021-2028), or is an interferon. In yet another embodiment, the agent for suppressing immune response comprises a population of co-polymer molecules having a subset of amino acid residues in random amino sequence. For example, the co-polymer is glatiramer acetate (also known as Copaxone™).

In some embodiments, the islet neogenesis therapy composition and the agent for suppressing immune response are administered simultaneously. Alternatively, the islet neogenesis therapy composition and the agent for suppressing immune response are administered sequentially. Simultaneous administration can be administering the . composition and the agent together in a combination, or separately and within about 15 min., about 30 min., or about one hour or even within about one day of each other. Sequential ) administration means that more than one about day elapses between administering the islet neogenesis therapy composition and the immunosuppressive agent, for example, about one week, about two weeks, or about six weeks elapses between administering the IN.T.™ composition and the immunosuppressive agent. } In a possible embodiments for treating diabetic patients, the immunosuppressive agent is administered over a course of about several days or about a week, and the IN.T.™ composition is administered later, for example, after a period of administration of neither the agent nor the composition. The period of administering neither the composition nor the agent can be used to monitor the patient to determine whether a change in immunosuppression in the patient can be observed. In an alternative embodiment, the

I.N.T.™ composition is administered over a course of about several days or about a week or about several weeks, and the immunosuppressive agent is administered later, for example, after a period of administration of neither the agent nor the composition. In yet another alternative embodiment, both the LN.T.™ composition and the immunosuppressive agent can be administered, and the LN.T.™ composition is then stopped while immunosuppression is continued. In yet another embodiment, the IN.T.™ composition and the immunosuppressive agent combination is administered, followed by treatment with a combination of a gastrin/CCK ligand and immunosuppressive agent. As used herein, the term “an” can also mean “at least one”, so that, for example, an immunosuppressive agent can be two or more immunosuppressive agents administered to effect successful suppression of immune reaction.

The islet neogenesis therapy composition is administered systemically, for example, is administered as a bolus, by a route selected from the group consisting of intravenous, subcutaneous (sc), intraperitoneal (ip), and intramuscular (im). Intravenous administration can be administered as an infusion (drip) or as a bolus. The agent for suppressing immune response can be administered orally, for example if the agent is an orally available drug.

Alternatively, the agent for suppressing immune response is administered systemically, for example, by a route selected from the group consisting of intravenous, subcutaneous, . intraperitoneal, and intramuscular. The agent for suppressing immune response can be, for example, at least one of Tacrolimus, Sirolimus, and daclizumab. : The method can further comprise measuring a physiological parameter in the subject such as the level of fasting blood glucose; pancreatic insulin content; pancreatic f cell content; and pancreatic islet content. The subject is a diabetic non-human mammal, for example, the subject is a non-obese diabetic (NOD) mouse. In the latter subject, the method . can further comprise evaluating the extent of survival in the absence of administration of insulin. Alternatively, the subject is a human, for example, the subject is a human patient, for example, is a human diabetes patient, for example, is a human with a form of diabetes, and is in need of treatment.

Another embodiment provided is a method of treating a diabetic subject, the method comprising administering to the subject a composition for islet neogenesis therapy consisting of an effective dose of at least one of EGF51N and gastrin 17, and administering an effective dose of at least one immunosuppressing agent. Yet another embodiment provided is a method of treating a diabetic subject, the method comprising administering to the subject a composition for islet neogenesis therapy consisting of an effective dose of at least one of

EGF51N and gastrin 17, and administering an effective dose of each of Tacrolimus and

Sirolimus. Yet another embodiment provided is a method of treating a diabetic subject, the method comprising administering to the subject a composition for islet neogenesis therapy consisting of an effective dose of at least one of EGF5IN and gastrin 17, and administering an effective dose of at least one of Tacrolimus, daclizumab, and Sirolimus. The composition or agent can further include a pharmaceutically acceptable buffer.

Also provided herein is a kit for treatment of a diabetic subject, comprising an immunosuppressive agent, an LN.T.™ composition, and a container. The kit can further comprise instructions for use.

A pharmaceutical composition for treating diabetes comprising a gastrin/cholecystokinin (CCK) receptor ligand and an agent for suppression of immune response is provided. The gastrin/CCK receptor ligand is, in one embodiment, a gastrin, for example, gastrin17Metl15, or gastrin17Leul5.

The pharmaceutical composition is formulated, for example, for parenteral . administration. Alternatively, the pharmaceutical composition is formulated for oral administration, formulated for parenteral administration, or formulated for a route of : administration selected from the group consisting of subcutaneous, intraperitoneal (i.p.), intravenous (i.v.), and intramuscular injection. The composition is formulated for systemic administration. The composition is formulated for a route of administration selected from the group consisting of transdermal and mucosal delivery. . A feature of the present invention is a method of treating a diabetic subject by administering to the subject a composition comprising a gastrin/CCK ligand receptor, and an agent for suppressing immune response. The agent can be a protein, for example, all ora portion of an antibody. Alternatively, the agent can be a drug, which as used herein means a low molecular mass compound, having a molecular weight of for example, less than about 5,000 daltons, less than about 2,000 daltons, or less than about 1,000 daltons.

Further, the gastrin/CCK receptor comprises gastrin 17, for example, the gastrin/CCK receptor ligand is human gastrinl7 having a leu at position 15, or having a met at position 15.

The agent for suppressing immune response can be at least one of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6-mercaptopurine; a FK506; ISAtx247; 15-deoxyspergualin; an FTY 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4-octylphenyl)ethyl]propane-1,3- diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; or a demethimmunomyein.

For example, the rapamycin is SDZ-RAD. For example, the corticosteroid is dexamethasone. Other agents capable of suppressing immune response are considered within the scope of the composition with use of a gastrin/CCK receptor ligand. Alternatively, the agent is the protein that comprises an amino acid sequence of an antibody. For example, the agent is at least one of: hul 124; BTI-322; allotrap-HLA-B270; OKT4A; Enlimomab; ABX-

CBL; OKT3; ATGAM; basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247;

Medi-500; Medi-507; Alefacept; efalizumab; or infliximab. In a different embodiment, the agent for suppressing immune response can be a naturally occurring cytokine or chemokine, such as an interferon or a glial protein such as GFAP or S100p. In yet another embodiment, the agent for suppressing immune response comprises a population of co-polymer molecules having a subset of amino acid residues in random amino sequence. For example, the co- . polymer is glatiramer acetate, or another copolymer comprising amino acids (see, for example, Fridkis-Hareli et al., 2002, J. Clin. Inves. 109:1635-1643). : In some embodiments, the gastrin/CCK receptor ligand and the agent for suppressing immune response are administered simultaneously. Alternatively, the gastrin/CCK receptor ligand and the agent for suppressing immune response are administered sequentially.

Simultaneous administration can be administering the gastrin/CCK receptor ligand and the agent together in a combination, or separately and within minutes, for example, 15 min., 30 min., or within hours, for example, one hour, four hours, or even within one day of each other. Sequential administration means that more than one day elapses between administering the gastrin/CCK receptor ligand and the agent for suppressing immune response. In an embodiment of a method for treating diabetic patients, the immunosuppressive agent is administered over a course of several days or about a week, and the gastrin/CCK receptor ligand is administered later, for example, after a period of administration of neither the agent nor the receptor ligand.

The period of administering neither the agent nor the receptor ligand is used to monitor the patient to determine whether a change in immunosuppression in the patient can be observed. In an alternative embodiment, both the gastrin/CCK receptor ligand and the agent that suppresses immune response can be first administered in combination, and then administration of the gastrin/CCK receptor ligand is discontinued and administration of the agent that suppresses immune response is continued. An alternative embodiment herein is a method of treating a diabetic patient comprising administering an I.N.T.™ composition for a period of time. The period of time may be at least one day, for example, scveral days, for example, at least one week, for example several weeks, for example, at least six weeks, followed by administration of at least one immunosuppressive agent.

The gastrin/CCK receptor ligand is administered systemically, for example, is administered as a bolus, by a route selected from the group consisting of intravenous, subcutaneous, intraperitoneal, and intramuscular. Intravenous administration can be administered as an infusion (drip) or as a bolus. The agent for suppressing immune response can be administered orally, for example if the agent is an orally available drug.

Alternatively, the agent for suppressing immune response is administered systemically, for . example, by a route selected from the group consisting of intravenous, subcutaneous, intraperitoneal, and intramuscular. The agent for suppressing immune response can be, for : example, at least one of Tacrolimus, Sirolimus, ISAtx247, and daclizumab.

J

The method can further comprise measuring a physiological parameter in the subject such as the level of fasting blood glucose; pancreatic insulin content; pancreatic 3 cell content; and pancreatic islet content. The subject is a diabetic non-human mammal, for example, the subject is a non-obese diabetic (NOD) mouse. In the latter subject, the method can further comprise evaluating the extent of survival in the absence of administration of insulin. Alternatively, the subject is a human patient, for example, with an indication of a form of diabetes, and in need of treatment.

Another embodiment provided herein is a method of treating a diabetic subject, the method comprising administering to the subject a gastrin/CCK receptor ligand consisting of an effective dose of gastrinl7, and administering an effective dose of at least one immunosuppressing agent. Yet another embodiment provided is a method of treating a diabetic subject, the method comprising administering to the subject a composition gastrin/CCK receptor ligand comprising an effective dose of at least one of gastrinl7, and administering an effective dose of each of Tacrolimus and Sirolimus. Yet another embodiment provided is a method of treating a diabetic subject, the method comprising administering to the subject a gastrin/CCK receptor ligand consisting of an effective dose of gastrinl7, and administering an effective dose of at least one of Tacrolimus, daclizumab,

ISAtx247, and Sirolimus. The compositions or agents herein can further include a pharmaceutically acceptable buffer.

Also provided herein is a kit for treatment of a diabetic subject, comprising an immunosuppressive agent, a gastrin/CCK receptor ligand, and a container. The kit can further comprise instructions for use. Any of the bits herein can further comprise a unit dose, i.e., an effective dose, of either or both of the composition and at least one agent therein.

Brief Description of the Drawings

Figure 1 is a line graph showing percent survival on the ordinate of NOD mice in each of four groups as described in Example 1, as a function of time in days on the abscissa.

Starting at week 4, Group 1 animals (solid circles) were administered concurrently an LN. T. : ™ composition (Gastrin and EGF) and immunosuppression drugs (Sirolimus and

Tacrolimus); Group 2 animals (solid squares) were administered the LN.T. ™ composition only; Group 3 animals (open squares) were administered the immunosuppression drugs only; and Group 4 animals (open circles) were administered vehicle only.

Figure 2 is a line graph showing the decrease in fasting blood glucose (FBG) at weeks 7 and 9 of surviving Group 1 animals as described in Example 1. Each line represents the data from an individual surviving mouse.

Figure 3 is a graph showing levels of pancreas insulin, in ng/g body weight, plotted as a function of blood glucose concentration in mM, of surviving mice from Example 1 (solid squares). Control data points include a 4 week point from an insulin treated non-diabetic

NOD mouse, a 12-15 week acute pre-diabetic NOD mouse, and a 15 week point from a chronic diabetic mouse (solid triangles). The data show that the blood glucose levels in

LN.T. ™/immunosuppressed mice per unit weight are lower than that of chronic diabetic mice.

Figure 4 is a set of two bar charts showing the levels of plasma C-peptide and pancreatic insulin, respectively, in recent onset diabetic NOD mice in each of four groups treated as described in Example 3, in samples taken from the mice on day 0 and day 28 of treatment. Group | animals were administered concurrently an I.N.T. ™ composition (Gastrin/EGF) and immunosuppression drugs (Sirolimus and Tacrolimus); Group 2 animals were administered the IN.T.™ composition only; Group 3 animals were administered the immunosuppression drugs only; and Group 4 animals were administered vehicle only.

Detailed Description of the Invention

The invention in one aspect features islet neogenesis therapy (ILN.T.™) compositions and methods, for example, gastrin and EGF in combination with immunosuppressive agents, to stimulate the growth of new 3 cells in vivo, increasing islet mass, and result in improved glucose tolerance in diabetic subjects, for example, in diabetic humans and in animals.

The gastrin/CCK receptor ligand and the EGF receptor ligand can be administered in a single combined dose, or administered separately in any order. An “effective combined . dose” of these compositions is one which produces an increase in number of insulin secreting cells, or an increase in insulin blood level, or an increase in [3-cell mass. The gastrin/CCK receptor ligand is, in one embodiment, human gastrin of length 17 amino acid residues, the residue at position 15 being leucine (1-17Leul5, referred to herein as gastrinl7leul5);

further, the EGF receptor ligand is human EGF51N. The effective dose can contain a ratio of gastrin/CCK receptor ligand to EGF receptor ligand that is greater than 1, for example, the effective dose contains a ratio of gastrin/CCK receptor ligand to EGF receptor ligand greater than 10. A convenient route of administering the dose is with a systemic injection, for example, a subcutaneous bolus.

In a further embodiment, the recipient subject is administered an agent that suppresses the immune system. For example, the agent is a low molecular weight organic chemical, for example, is at least one of Tacrolimus, Sirolimus, cyclosporine, and cortisone and other drugs as shown in Table 1. In an alternative embodiment, the agent is an antibody, for example, the antibody is anti-CD1 la and other antibodies also shown in Table 1. In yet another alternative, the immunosuppressive agent can be an antibody that is elaborated by the subject following an immunization schedule, for example, against GFAP or against S100.

The subject can be diabetic, for example, the subject is a non-obesc diabetic mouse (the NOD mouse) or a streptozoticin-treated mouse. The subject can be a human, for example a diabetic patient having type I or type II diabetes, or having gestational diabetes, or having had diabetes in the past, for example, having had gestational diabetes in a past pregnancy.

Further, evaluating the size and function of newly developed B insulin secreting cells or islets is measuring a parameter selected from the group of: islet B cell mass, islet f cell number, islet $ cell percent, blood glucose, serum glucose, blood glycosylated hemoglobin, pancreatic B cell mass, pancreatic f3 cell number, fasting plasma C peptide content, serum insulin, and pancreatic insulin content.

As diabetes is in certain cases an autoimmune disease, an embodiment of IN.T. ™ is systemic administration of therapeutically effective doses of, for example, the ligands of receptors for each of EGF and gastrin/CCK, such as a combination of a gastrin and an EGF, to subjects or patients who are also treated with one or more agents that suppress the immune system, i.e., immunosuppressive agents. The gastrin and EGF combination would be . administered systemically as described for the treatment of diabetic patients where the goal is to stimulate neogenesis of islets formed de novo within the pancreas (see U.S. patent : numbers 5,885,956 and 6,288,301).

A number of different endpoints can be used to determine whether gastrin and EGF : treatment, or treatment with the combination of gastrin and EGF and an immunosuppressive agent, increases the functional mass of 8 cells in the islet transplants. These include measurement of enhanced plasma levels of circulating human C peptide and human insulin, after injecting mice with {8 cell stimulants such as glucose or arginine; a response to gastrin/EGF treatment demonstrated by increased human insulin immunoreactivity or mRNA levels extracted from the islet transplants; and increased number of cells, determined by morphometric measurement of islets in treated animals. Enhanced f cell function of human islets can also be demonstrated by reversal of the hyperglycemia in recipient mice with streptozotocin induced or genetic (NOD) diabetes. In Examples herein, enhanced f cell function after treatment of the diabetic recipient subject with gastrin, with EGF and with one or more Immunosuppressive agents was demonstrated by improved survival upon withdrawal of insulin, and by correcting hyperglycemia as indicated by fasting blood glucose level.

Further, increases in both pancreatic insulin and plasma C-peptide were observed.

. Table I. Exemplary agents for immune suppression, and commercial sources 2-amino-1,3-propanediol | Novartis Used for preventing or derivatives treating chronic rejection in a patient receiving an organ or tissue allo-or xeno- transplant 2-amino-2[2-(4- Yoshitomi Immunosuppression, from octylphenyl)ethyl]propane- | Pharmaceutical accelerated lymphocyte 1,3-diol hydrochloride Industries, Ltd homing 40~O-(2-hydroxyethyl)- Novartis Sirolimus (rapamycin) rapamycin, SDZ-RAD, Pharmaceuticals derivative, used for acute

Everolimus (Certican®) kidney rejection; reduces rejection and graft vasculopathy following heart transplantation by inhibiting cell proliferation 6-(3-dimethyl- Matsumori Akia Immunosuppressing action aminopropionyl) forskolin | Nippon Kayaju Co useful also for treating

Ltd autoimmune disease 6-mercaptopurine Glaxo SmithKline Used to treat Crohn's (Purinethol®, 6-MP) disease, inflammatory bowel disease and for organ transplant therapy

ABX-CBL (CBL-1) Abgenix Mouse monoclonal AB targeted against human T- cell, B cells, NK cells and monocytes, fortreatment of steroid-resistant graft vs host diseases, potential use in treatment of inflammatory and autoimmune disorders

Alefacept (human LFA-3 | University of Utah- Knocks out causative

IgG1 fusion protein, Dermatology memory T-lymphocytes;

AMEVIVE®) Dept/BIOGEN . | Used to treat psoriasis, a T- cell mediated inflammatory disorder

HLA-B2702 peptide SangStat Medical Human peptide, blocks (Allotrap ®) action of NK cells and T- cell mediated toxicities, used for prevention of first

Wo ositszor PCT/US03/18377

Tm Tiddneyallograft rejection

Antisense ICAM-1 ISIS-Boehringer Mouse monoclonal AB inhibitor (ISIS 2302), Ingleheim blocks white blood cell

Enlimomab, BIRR1, adhesion to T-cell surface : Alicaforsen) molecule (ICAM-1r); treatment of kidney transplant rejection

Azathioprine (Imuran®, Generic, Glaxo Treatment of rheumatoid

Azasan®) SmithKline, arthritis and prevention of

Prometheus kidney transplant rejection,

Laboratories, and other autoimmune or aaiPharma inflammatory disorders such as inflammatory bowel disease

BTI-322 MedImmune Mouse derived monoclonal

AB targeted to CD2 receptor; used for prevention of first-time kidney rejection, and treatment of resistant rejection

Cladribine (Leustatin®) Boehringer Antimetabolite and

Ingleheim immunosuppressive agent that is relatively selective for lymphocytes; used to treat lymphoid malignancies, e.g., hairy- cell leukemia.

Cyclophosphamide (CTX, | Generic Immunosuppressant t for

Neosar®, Cytoxan®, treatment of arthritis and

Procytox®) other auto-immune disorders and cancers

Cyclosporine (cyclosporin | Novartis 11 amino acid cyclic

A, cyclosporin) peptide; blocks helper T- (Sandimmune®, Neoral®, cell , immunosuppressant

SangCya®) used in organ transplant therapy and other immune diseases } Demethimmunomycin" (L- | Merck & Co Treatment of autoimmune 683,742: also described as diseases, infectious diseases 31-desmethoxy-31- and/or prevention of organ : hydroxy-L-683,590) transplant rejections

Dexamethasone Generic An adrenocorticoid, (Decadron, Dexone, effective

Dexasone) immunosuppressant in

TT [variousdisorders : Docosahexaenoic acid Not applicable Immunosuppressant by that (DHA) lowers the proportion of T cells expressing CD4 or ’ CDS, blocks antigen recognition process;

Taku et al., Journal of

Agricultural and Food

Chemistry, 2000; 48(4):1047

FTY720 (oral myriocin Novartis Alters lymphocyte derivative) Pharmaceuticals infiltration into grafted tissues; used for prevention of organ rejection in kidney transplants

Glatiramer acetate (co- Teva Synthetic peptide polymer-1, Copaxone®) Pharmaceuticals copolymer; decoy that mimics structure of myelin so immune cells bind

Copaxone instead of myelin; for multiple ] sclerosis

Glial fibrillary acidic CalBiochem; Synx Possesses protein (GFAP) Pharma immunosuppressive activities in diabetic animal models; Winer et al., Nature

Medicine 9: 198 (2003)

Gusperimus,(15- Bristol Myers-Squibb | Intravenous deoxyspergualin (Spanidin immunosuppressant; ®) suppresses production of cytotoxic T-cells, neutrophils and macrophages hul124 (anti-CD11a) XOMA Humanized monoclonal antibody; targets CD11a receptor on surface of T cells to selectively inhibit immune system rejection of transplanted organs

Infliximab (Remicade®) Centocor (affiliate of | Monoclonal AB, binds and

Johnson and inactivates human TNF- : Johnson) alpha and ; used to treat

Crohn’s disease and rheumatoid arthritis ‘Interferon | Various companies | Immunomodulatory

I ai

Biogen etc

ISAtx247 Isotechnika Used to treat autoimmune diseases such as rheumatoid : arthritis and psoriasis isotretinoin Immunosuppressant, reduces ability of T cells to proliferate in response to immune challenge.

Vergelli et al.,

Immunopharmacology, 1997, 31:191.

Medi-500 (T10B9) MedImmune Intravenous monoclonal AB that targets human T-cells; treats acute kidney rejection and graft-vs-host disease

Medi-507 MedImmune/Bio- Intravenous humanized AB

Transplant directed against CD2 T-cell; used to treat corticosteroid- resistant graft vs host disease and prevention of kidney rejection

Methotrexate Wyeth Lederle, Antimetabolite used to treat (Rheumatrex®, Generic Crohn’s disease, severe

Amethopterin, Trexall®) psoriasis, and adult rheumatoid arthritis (and as an anti-cancer drug)

Mitoxantrone Immunex Antiproliferative effect on (Novantrone®) cellular immune system including T-cells, B-cells and macrophages; used to treat hormone-refractory prostate cancer, acute myelogenous leukemia and multiple sclerosis mycophenolate mofetil Roche Proliferation of T and B (CellCept®) lymphocytes by blocking the synthesis of purine nucleotides; used in organ transplant therapy and inflammatory bowel disease . OKT4A R.W.Johnson Mouse monoclonal AB

Pharmaceutical targeted against human :

Research Institute CD4 T cell; used for prevention of kidney transplant rejection when used in combination with other immunosuppressant drugs : Muromonab-CD?3 R.W. Johnson Monoclonal AB that binds (Orthoclone OKT3 ® )() Pharmaceutical to receptor sites on T-cells,

Research Institute preventing activation by transplanted tissue

Prednisolone (Deltasone®, Corticosteroid, suppresses

Oraone®) inflammation associated with transplant rejection basiliximab (Simulect®) Novartis Monoclonal AB that binds

Pharmaceuticals to receptor sites on T-cells, preventing activation by transplanted tissue (renal transplant)

S1008 glial protein Possesses immunosuppressive activities in diabetic animal models

Sirolimus, Rapamycin Wyeth-Ayerst Immunosuppressant and (Rapamune ®) Laboratories potent inhibitor of cytokine (e.g.IL-2)-dependent T-cell proliferation (kidney transplant) 506) communication

Antithymocyte SangStat Medical Anti-human thymocyte immunoglobulin Corporation, immunoglobulin; used in (ATGAM, Thymoglobulin | Pharmacia and reversal of acute kidney ®) Upjohn transplant rejection and will likely be used off-label for transplant induction therapy efalizumab (Xanelim ©) XOMA T-cell modulator that target

T-cells through interactions with adhesion molecules on endothelial cell surface, target migration of T-cells into the skin and target activation of T-cells; Used to treat Psoriasis : Daclizumab (Zenapax®), | Protein Design Monoclonal AB inhibits

HAT (Humanized Anti- Laboratories/Roche | binding of IL-2 to IL-2

Tac), SMART anti-Tac, receptor by binding to IL-2 anti-CD25, and humanized receptor; suppresses T cell

(renal transplant)

The methods and compositions herein increase the confidence that clinical trials of treatment with gastrin and EGF and an immunosuppressive agent will show stimulation of endogenous § islet cell growth within the pancreas in type I and type II diabetic patients.

As used herein, the term “gastrin/CCK receptor ligand” encompasses compounds that stimulate the gastrin/CCK receptor such that when EGF receptors in the same or adjacent tissue or in the same individual are also stimulated, neogenesis of insulin-producing pancreatic islet cells is induced. Examples of such gastrin/CCK receptor ligands are given in

U.S. patent 6,288,301, and include various forms of gastrin, such as gastrin 34 (big gastrin), gastrin 17 (little gastrin), and gastrin 8 (mini gastrin); various forms of cholecystokinin such as CCK 58, CCK 33, CCK 22, CCK 12 and CCK 8; and other gastrin/CCK receptor ligands that demonstrate the same synergistic activity with EGF receptor ligands, and which can induce differentiation of cells of mature pancreas to form insulin-secreting islet cells, when acting synergistically with an EGF receptor ligand. Also contemplated are active analogs, fragments and other modifications of the above, including both peptide and non-peptide agonists or partial agonists of the gastrin/CCK receptor such as A71378 (Lin et al, Am. J.

Physiol. 258 (4 Pt 1): G648, 1990) that either alone or in combination with EGF receptor ligands induce differentiation of cells in mature pancreas to form insulin-secreting islet cells.

Small forms of gastrin such as gastrin 17 are economically prepared by peptide synthesis, and the synthetic peptides are commercially available. Synthetic human gastrin 17, and derivatives such as human gastrin 17 having leucine in place of methionine at position 15 as used in Examples herein, are also available from Bachem AG, Bubendorf,

Switzerland, and from Researchplus, Nanasquan, New York.

Gastrin/CCK receptor ligands include also active analogs, fragments and other modifications of the above ligands. Such ligands also include compounds that increase the secretion of endogenous gastrins, cholecystokinins or similarly active peptides from sites of tissue storage. Examples of these are the gastric releasing peptide, omeprazole which inhibits gastric acid secretion, and soya bean trypsin inhibitor which increases CCK stimulation.

As used herein, the term “EGF receptor ligand” encompasses compounds that : stimulate the EGF receptor such that when gastrin/CCK receptors in the same or adjacent tissue or in the same individual are also stimulated, neogenesis of insulin-producing pancreatic islet cells is induced. Examples of such EGF receptor ligands include full length

EGF which is EGF1-53, and further include EGF 1-48, EGF1-49, EGF1-52, and fragments and active analogs thereof. Other examples of EGF receptor ligands are TGFa forms that include 1-48, 1-47, 1-51, and amphiregulin and pox virus growth factor as well as any EGF receptor ligands that demonstrate the same synergistic activity with gastrin/CCK receptor ligands. These include active analogs, fragments and modifications of the above. See also,

Carpenter and Wahl, Chapter 4, in Peptide Growth Factors (Eds. Sporn and Roberts),

Springer Verlag, 1990.

The group of compounds which are EGF receptor ligands further includes “modified

EGF”, which are variants of normal or wild type EGF. Modifications have been shown to affect one or more biological activity such as the rate of clearance of EGF. The term includes peptides having an amino acid sequence substantially similar to that of human EGF, for example, with one or a few amino acid substitutions at various residue positions.

Recombinant EGF forms have been genetically engineered to have alterations in structure and activities, for example, EGF having a methionine at position 21 replaced by a leucine residue has been described (U.S. patent number 4,760,023). Recombinant human

EGF (hEGF) having 51 residues, i.e., lacking the two C-terminal residues at positions 52 and 53 of hEGF, and having a neutral amino acid substitution at position 51, retain EGF activity and are more resistant to protease degradation during a microbial production process, and following administration to a subject. A series of nucleic acid molecules have been described that encode a family of proteins that have significant similarity to EGF and TGFa (WO 00/29438). EGF muteins (mutated EGF) having histidine at residue 16 replaced with a neutral or acidic amino acid have been described (WO 93/03757), such forms retaining activity at low values of pH. Chemical analogues and fragments of EGF and TGF retain ability to bind various members of the EGF receptor family (U.S. patent number 4,686,283).

Various modifications of EGF or TGFa confer advantageous properties affecting one or more of recombinant protein production, in vitro and in vivo stability, and in vivo activity. A preferred recombinant modified EGF receptor ligand used in the Examples herein is a C- terminus deleted form of human EGF of 51 amino acids in length, having asparagine at position 51 (referred to herein as EGF51N), which EGF protein retains substantially full

IN.T.™ activity, and has in vivo and/or in vitro stability that is that is at least about as great or greater than normal or wild type hEGF (U.S. patent application 10/000,840, S. Magil et al., published May 12, 2003 as PCT/US02/33907, and incorporated by reference herein in its entirety).

As used herein, the term “immunosuppressant” means any agent that suppresses immune response. Exemplary immunosuppressant agents are shown in Table 1, and any derivatives of those agents or functional equivalents are considered appropriate for embodiments of the invention as described herein and in the claims.

As used herein, a dosing schedule refers to a protocol for administering an IN.T.™ composition and one or more of an immunosuppressive agent, each in an effective dose, administered together or separately, and includes the amount of the composition delivered per day, and the duration or period of time over which each composition is administered.

Most insulin dependent diabetic patients require insulin injection at least on a daily basis. Multiple doses per day of insulin are required under certain circumstances of illness or diet for management of diabetes, and the insulin administration is indicated by results of frequent glucose monitoring, another activity which is required of a diabetes patient for optimal management of the disease, which is performed for example as often as five times daily.

Remission from diabetes due to successful islet neogenesis therapy in combination with an immunosuppressive agent is indicated by a decreased fasting blood level of glucose, and by a decreased level and duration of elevated blood glucose in response to a dietary challenge of sugar consumption. Upon achieving successful islet neogenesis, insulin administration is reduced from, for example, five injections to two injections per day; from ) two injections to one injection per day; and from one to none, as indicated by data obtained from monitoring blood glucose levels. One of ordinary skill in the art of diabetology, when treating a diabetic patient, is familiar with adjusting insulin dosage to levels of blood glucose following fasting and under other physiological conditions.

Dosages of the LN.T.™ compositions to be administered to a subject are adjusted for : known variations from species to species in standard data encompassing criteria for absorption, distribution, half-life kinetics in circulation, metabolism, excretion, and toxicology of the receptor ligands of the embodiments herein, for example, for each primate and rodent species. In general, dosages are adjusted to be about 6-fold to about 100-fold greater for administration to a rodent species than to a primate species.

Immunosuppressive agents in Table 1 or other equivalent agents are administered as supplied by the manufacturers, normalizing to body weight of the subject as is known by one of skill in the pharmacological arts. For example, Tacrolimus is generally administered by injection or orally, Sirolimus is generally administered orally.

Modes of administration of the receptor ligand compositions and immunosuppressive agents include but are not limited to subcutaneous, transdermal, intramuscular, intraperitoneal, intravenous, intranasal, and oral routes. The compounds may be administered by any convenient route, for example by infusion or bolus injection, by pump, by absorption through epithelial or mucocutaneous linings (e.g., oral mucosa, rectal and intestinal mucosa, etc.). The receptor ligands herein may be administered in combination with one or a plurality of other biologically active agents. For example, a recipient of the compositions and methods herein may be administered one or more antibiotics if a bacterial infection is present, or aspirin if a headache is present. Administration of the receptor ligands herein is preferably by a systemic route.

The present invention also provides pharmaceutical compositions. Such compositions comprise a therapeutically effective amount of a therapeutic, and a pharmaceutically acceptable sterile carrier or excipient. Such a carrier includes but is not limit to saline, buffered saline, dextrose, water, glycerol, ethanol, and combinations thereof. The formulation should suit the mode of administration.

The composition, if desired, can also contain minor amounts of wetting or emulsifying agents, such as Tween, or pH buffering agents. The composition can be a liquid solution, suspension, emulsion, tablet, pill, capsule, sustained release formulation, or powder.

The composition can be formulated as a suppository, with traditional binders and carriers such as triglycerides. Oral formulation can include standard carriers such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, ’ magnesium carbonate, etc.

In one embodiment, the composition is formulated in accordance with routine procedures as a pharmaceutical composition adapted for intravenous administration to human beings. Compositions for systemic administration are typically solutions in sterile isotonic aqueous buffer. Where necessary, the composition may also include additional components, for example, a solubilizing agent. Generally, the ingredients are supplied either separately or pre-mixed in unit dosage form. In addition to solution form, the composition can be supplied as a dry lyophilized powder, or as a non-aqueous concentrate, for example, in a hermetically sealed container such as an ampoule or sachette indicating the quantity of active agent.

Where the composition is to be administered by infusion, it can be dispensed using an infusion bottle containing sterile pharmaceutical grade water or saline. Where the composition is administered by injection, an ampoule of sterile water or saline for injection can be provided so that the ingredients may be mixed prior to administration.

The therapeutic agent embodiments of compositions of the invention can be formulated as neutral or salt forms. Pharmaceutically acceptable salts include those formed with free amino groups such as those derived from hydrochloric, phosphoric, acetic, oxalic, tartaric acids, etc., and those formed with free carboxyl groups such as those derived from sodium, potassium, ammonium, calcium, ferric hydroxides, isopropylamine, triethylamine, 2- ethylamino ethanol, histidine, procaine, etc.

The amount of each of the therapeutic agents of the compositions herein, and their relative amount with respect to each other which will be effective in the treatment ofa particular disorder or condition will depend on the nature of the disorder or condition, and can be determined by standard clinical techniques. The precise dose to be employed in a formulation depends also on route of administration, and the extent of the disease or disorder in a given patient, and should be decided according to the judgment of the practitioner and each patient’s circumstances. However, suitable dosage ranges for parenteral administration are generally about 0.01 microgram or one microgram, to about 100, about 200, or to about 500 micrograms of the active compound per kilogram body weight for an EGF receptor ligand. Preferably, the dose range of the EGF receptor ligand is 0.01 micrograms to 100 micrograms per kg body weight. Further, suitable dosage ranges for parenteral administration are generally about 0.1 micrograms or about 0.2 or about 0.5 micrograms to about 2 milligrams, or about 3 milligrams, or to about 5 milligrams per kg body weight for a gastrin receptor ligand. Preferably, the dose range of the gastrin receptor ligand is about 0.1 microgram to 2 milligram per kg body weight.

Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems. A daily dose is administered as a single dose or divided into a plurality of smaller fractional doses, to be administered several times during the day.

Suppositories generally contain active ingredient in the range of about 0.5% to about 10% by weight; oral formulations preferably contain about 10% to about 95% active ingredient by weight.

Embodiments of the invention herein also provide a pharmaceutical pack or kit comprising one or more containers filled with one or more of the ingredients of the pharmaceutical compositions of the invention, for example, a container having a unit dosage of each or both of a gastrin/CCK receptor ligand and an EGF receptor ligand, and one or more of an immunosuppressive agent. Associated with such container(s) can be a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use or sale for human administration. The pack or kit can in certain embodiments include one or more containers, for example, containers having insulin, to be administered, for example, during the dosing schedule prior to induction of islet neogenesis and remission from diabetes.

Without being limited by any particular model or mechanism, it is anticipated that an early onset of diabetes is characterized by a different stage of islet destruction, for example, having a different population of pancreatic cells than is late stage diabetes. For example, it is likely that the early onset diabetes or prediabetic patient has a functional p cell mass and a ligher level of islet cell precursor cells, populations which are likely to be lower, or deficient or lacking in a late stage type I diabetic. For that reason, the invention in another general embodiment provides a method for preventing onset of diabetes or treating diabetes, for example early onset diabetes, the method comprising administering to a mammal in need thereof a composition comprising of a gastrin/CCK receptor ligand and an agent that : suppresses an immune response. Further, it is anticipated that such a treatment can be used over a long time period, for prolonged maintenance of the diabetic patient.

The invention in one aspect features a method for expanding a pre-existing functional 8 cell mass within the subject’s islets, or of expanding a functional f cell mass within pancreatic islet transplants in a diabetic mammal recipient of a transplant of purified islets.

The method comprises administering a composition comprising an effective dose of each of a gastrin/CCK receptor ligand and agent that suppresses an immune response.

Gastrin in combination with injury can stimulate the growth (proliferation) of new 8 cells, resulting in increased islet mass (Rooman, I., et al., 2002, Diabetes 51(3), 686-690).

Gastrin can effect differentiation of a pancreatic islet precursor cell to a mature insulin- producing cell, thus enhancing islet neogenesis and reversing diabetes symptoms or inhibiting progression of the diabetes.

The gastrin/CCK receptor ligand and immunosuppressive agent can be administered ina single combined dose, or administered separately in any order. An “effective combined dose” of these compositions is one which produces an increase in number of insulin secreting cells, or an increase in insulin blood level, or an increase in B-cell mass. The gastrin/CCK receptor ligand is in onc embodiment, human gastrin of length 17 amino acid residues, the residue at position 15 being leucine (gastrinl17Leul5); or methionine (gastrinl 7Met15). A convenient route of administering the dose is with a systemic injection, for example, a subcutaneous bolus.

The subject is administered the gastrin/CCK receptor ligand and an agent that suppresses the immune system. In one embodiment, the agent is a low molecular weight organic chemical, for example, is at least one of Tacrolimus, Sirolimus, ISAtx247, cyclosporin, and cortisone and other drugs as shown in Table 1. In an alternative embodiment, the agent is an antibody, for example, the antibody is anti-CD11a and other antibodies also shown in Table 1. The subject can be a diabetic mammal, for example, a non-obese diabetic mouse, the NOD mouse. The subject can be a human diabetic patient, for example having type I or type II diabetes, or having gestational diabetes, or having had gestational diabetes in the past. Further, evaluating the size and function of newly developed

B insulin secreting cells or islets is measuring a parameter selected from the group of: islet B : cell mass, islet B cell number, islet B cell percent, blood glucose, serum glucose, blood glycosylated hemoglobin, pancreatic f cell mass, pancreatic B cell number, fasting plasma C peptide content, serum insulin, and pancreatic insulin content.

As diabetes is in certain cases an autoimmune disease, an embodiment is systemic administration of therapeutically effective doses of, for example, a ligand of a receptor for a gastrin/CCK, to subjects or patients who are also treated with onc or more agents that suppress immune response (see Table 1).

A number of different endpoints can be used to determine whether treatment with gastrin and the agent that suppresses an immune response increases the functional mass of p cells in the islet transplants. These include measurement of enhanced plasma levels of circulating human C peptide and human insulin, after injecting mice with B cell stimulants such as glucose or arginine; a response to treatment with gastrin and the immunosuppressive agent demonstrated by increased human insulin immunoreactivity or mRNA levels extracted from the islet transplants; and increased number of B cells, determined by morphometric measurement of islets in treated animals. Enhanced B cell function of human islets can also be demonstrated by reversal of the hyperglycemia in recipient mice with streptozotocin induced or genetic (NOD) diabetes.

The methods and compositions herein increase the confidence that clinical trials of treatment with gastrin and an immunosuppressive agent will show stimulation of endogenous

B islet cell growth within the pancreas in type I and type II diabetic patients.

The methods herein are also valuable in determining the plasma levels of various compositions, such as the administered gastrin/CCK receptor ligand that stimulates new growth of human islets in vivo, or another endogenous factor that is induced by administered gastrin/CCK receptor ligand. In combination with Phasc I pharmacokinetic data, data obtained using these methods can improve the design of clinical studies, by reducing dose exploration studies in human subjects, to determine the effective human dose range.

The agent that suppresses the immune system or the gastrin/CCK receptor ligand is provided in an amount that is sufficient in combination to induce differentiation of the pancreatic islet precursor cells into insulin secreting islet cells for a prolonged period of time.

In general, for treatment with gastrin/CCK receptor ligand and an immunosuppressive : agent, as for any of the embodiments herein, the subject can be a mammal such as a human.

In addition, the mammal can be a rodent such as a mouse or rat, or a dog, cat, sheep, goat, cow, horse, or an ape such as a gorilla or a chimpanzee.

In another aspect, the invention provides a method for inducing pancreatic islet neogenesis in a mammal, the method comprising administering to the mammal a composition comprising a gastrin/CCK receptor ligand and an agent that suppresses the immune system, each in an amount sufficient to increase cell differentiation of islet precursor cells and inhibit islet destruction in the in pancreas, thereby having a net increase in pancreatic islet neogenesis.

The composition is in a dosage effective for inducing differentiation of an islet precursor cell into a mature insulin secreting cell. The composition can be in a pharmaceutically acceptable carrier, pharmaceutically acceptable buffer, or in a pharmaceutically acceptable salt.

In another aspect, the invention provides a kit for treating or preventing diabetes, containing a composition comprising a gastrin/CCK receptor ligand and an agent that suppresses the immune system, a container, and instructions for use. The composition of the kit can further comprise a pharmaceutically acceptable carrier. The composition of the kit can be present in at least one unit dosage. The kit can comprise insulin in at least one unit dosage.

As used herein, a dosing schedule refers to a protocol for administering a gastrin/CCK receptor ligand and at least one immunosuppressive agent, each in an effective dose, administered together or separately, and includes the amount of the composition delivered per day, and the duration or period of time over which each composition is administered.

Unless otherwise defined, all technical and scientific terms herein have the same : meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice of the present invention, suitable methods and materials are described below. All publication, patents, and patent applications mentioned herein are incorporated by reference in their entirety. The examples and claims herein are for illustrative purposes ; and are not intended to be further limiting.

EXAMPLES

Example 1: Survival of animals treated with an islet neogenesis composition and with a composition for immunosuppression.

Using a mouse model herein for chronic diabetes, the NOD mouse, subjects were tested for the effects of agents of immune suppression in conjunction with a composition for

LN.T.™ which is EGF5IN and gastrin 17leul5. The design of the experiment in shown in

Table 2. Treatments were initiated when subjects were about 25 weeks of age. Subjects were placed into four treatment groups: administration of LN.T.™ composition (Group 2), the immunosuppressive agents (Group 3), the combination of both (Group 1) or a control group treated only with the vehicle buffer (Group 4). Severely diabetic subjects having blood glucose levels greater than 30 mM (and ketosis) received insulin treatment, administered as a once daily sc injection of a 1:1 mixture of regular (R) pork insulin and

NPH (N) beef insulin (0.4 U of total insulin) for 3-4 weeks pretreatment and for 6 weeks during treatment. The protocol for administration of these agents following insulin pre- treatment are shown in Table 2.

Table 2. Concurrent Adminstration of Sirolimus and Tacrolimus immunosuppression with

LN. T.™

Group 1: Weeks 1-6: treat sc 0.4 U R&N beef/pork insulin daily n=16 Weeks 1-9: Gastrin 30 ng/kg/EGF 15 pg/kg ip twice daily

Weeks 1-9: Sirolimus 0.1 mg/kg, Tacrolimus 0.1 mg/kg oral daily

Group 2: Weeks 1-6: treat sc 0.4 U R&N beef/pork insulin daily n=12 Weeks 1-9: Gastrin 30 pg/kg/EGF 15 pg/kg ip twice daily

Weeks 1-9: vehicle oral daily

Group 3: Weeks 1-6: treat sc 0.4 U R&N beef/pork insulin daily n=3_§ Weeks 1-9: vehicle oral daily

Weeks 1-9: Sirolimus 0.1 mg/kg, Tacrolimus 0.1 mg/kg oral daily

CL.

Group 4: Weeks 1-6: treat sc 0.4 U R&N beef/pork insulin daily n=9 Weeks 1-9: vehicle oral daily

Weeks 1-9: vehicle oral daily 40 composition prolonged survival of mice previously treated with insulin therapy (Figure I;

Group 2, closed squares). Half of these subjects survived the 6 week course of insulin and . LN.T.™ (Group 2), compared to 11% of subjects treated only with insulin (Group 4, open 45 circles). LN.T.™ yielded about the same survival as treatment with insulin and immunosuppression with Sirolimus and Tacrolimus (Group 3, open squares). Treatment of

Group 1 mice with both LN.T.™ and immunosuppression following administration of insulin resulted in complete survival (100%) of the cohort for the entire 6 week duration of the treatment (Group 1, closed circles).

At one week after withdrawing administration of insulin, 56% in the LN.T.™ and concurrent immunosuppression group survived, compared to 11% in the insulin-treated vehicle control group. There were no survivors of administration of LN.T.™ alone or of administration of immunosupression alone after one week. At three weeks after withdrawal of insulin treatment, 31% of the animals of Group 1 receiving 1.N.T.™ and concurrent immunosuppression survived, no survivors among the Group 4 animals receiving the vehicle control.

The fasting blood glucose levels in the Group 1 surviving mice in the ILN.T.™ and immunsuppression group showed a significant decrease over the three week period following withdrawal of insulin treatment (Figure 2 data).

Table 3. Survival without insulin treatment at week 7 and 9.

Concurrent administration of Gastrin/EGF and Sirolimus/Tacrolimus.

Survival # FBG mM Survival # | FBG mM (%) Mean + SE (%) Mean + SE . ™

Group 1: IN.-T.™Mand IS) g 56, 18.74 1.9 531) 123%2 (n=16)

Group 2: LN.T.™ nob oo [0

Group 3: IS oo

Group 4: Vehicle

From these data obtained with NOD mice with chronic severe diabetes receiving insulin replacement therapy, administration of an LN.T.™ composition alone prolonged survival, and was more cffective than administration of agents of immunosuppression alone.

Complete survival for the 6 week periodof insulin replacement therapy was seen in the group receiving concurrent LN. T.™ treatment and immunosuppression. At 3 weeks after withdrawing insulin therapy, only the Group 1 animals administered IN.T.™ and concurrent immunosuppression for 7 weeks demonstrated significant survival (31%; Table 3). Further, surviving mice showed a significant decrease in blood glucose levels over the 3 weeks

Example 2. Pancreatic insulin content of chronically diabetic mice after treatment with an LN.T.™ composition and an immunosuppressants.

Table 4 contains reference data showing the time course of changes in fasting blood glucose (FBG) and pancreatic insulin content in samples obtained from female NOD mice, as a function of increasing age in of the subjects of this strain of mice. A relationship between these parameters is observed.

These data show that prior to observing a significant rise in blood glucose in NOD mice, a significant B cell loss has occurred, as indicated by the rapid decline in pancreatic insulin content. By age 10-12 weeks, in pancreatic insulin content decreased 37%, even though FBG remained normal at 4.6 mM. Further, only modest hyperglycemia (FBG 7.4 mM) in NOD mice was observed between ages of 12-15 weeks, despite profound § cell destruction associated with a pancreatic insulin content reduced by greater than 95%.

Further, when blood glucose rose to above 30 mM, the observed pancreatic insulin content was less than 0.1% that of the normal pancreatic insulin content.

These data indicate that a highly significant reduction in blood glucose might be obtained as a result of only a modest amount of cell regeneration, such that regeneration would increase pancreatic insulin content by no more than 3%, if B cell loss due to an autoimmune destruction can also be arrested.

Pancreatic insulin content in Female NOD mice as a function of age.

Table 5 shows the pancreatic insulin content of mice from Example 1 after withdrawal of insulin therapy. Surviving mice were sacrificed at 3 weeks (survivors in

Group 1 n=5), or earlier if moribund due to hyperglycemic diabetic ketoacidosis (Group 1 non-survivors, and all mice from Groups 2 and 3).

The data in Table 5 show that pancreatic insulin content in non-survivors of Group 1 and Groups 2 and 3 showed no increase from starting values of 44 ng/g for untreated animals (FBG levels of age 12-15 weeks diabetic NOD animals, FBG > 30 mM). However, there was a significant (about 30 fold) increase in the pancreatic insulin content in the surviving mice in the group receiving administration of IN. T.™ with concurrent immunosuppression (Group 1).

Table 5 from Example 1: Pancreatic insulin content for the different treatment groups

Group 1: INTIS | 0 ivors, FBG 12.3 £32) | 1,342 + 603 n=5

Among the surviving mice, the observed increase in pancreatic insulin content correlates with the observed reduction in blood glucose levels (Figure 3). The two individual subject mice having the highest pancreatic insulin levels (>1000 ng/g) each achieved a fasting blood glucose level in the non-diabetic range (3-6.6 mM).

Example 3: Pancreatic insulin content and plasma C-peptide levels of recent onset diabetic mice after treatment with an islet neogenesis composition and with a composition for immunosuppression. : Non-obese diabetic mice were therefore monitored for an initial appearance of diabetes, by a method involving daily morning testing, to obtain evidence of glucosuria.

Monitoring was initiated at 10 weeks of age, and symptoms of glucosuria were assessed using the Keto-Diastix (Bayer). Further, at the time of onset of glucosuria, subjects were further monitored for onset of diabetes by measuring a level of fasting blood glucose (FBG).

Using this assay, achieving a level of FBG greater than 6.6 mmol/l for a subject on two consecutive days was selected as defining the onset of diabetes in that subject.

By using this approach of daily monitoring for diabetes among 800 to 1000 female

NOD mice simultaneously, NOD mice were collected within 2-5 days after onset of diabetes.

These mice, having ages of 16-22 weeks, and having FBG levels of 15-17 mmol/l (mean, 15 mmol/l), were randomly distributed into four treatment groups. The groups then received treatments as follows:

Group 1: vehicle (untreated control, n= 9)

Group 2: IS (immunosuppressive drugs Sirolimus and Tacrolimus, n= 12)

Group 3: LN.T. (EGF and gastrin, n=8)

Group 4: IN.T. and IS (EGF and gastrin; and Sirolimus and Tacrolimus, n= 7)

Sirolimus (0.1mg/kg/day) and Tacrolimus (0.1mg/kg/day) were administered once a day in the morning by gavage in MCT oil (medium chain triglyceride oil, available as a food supplement). Treatment protocols were initiated at the onset of diabetes, and terminated after 14 days. Each of EGF (EGF51N, 3 pg/kg/day) and Gastrin (gastrin17leuls, 10 pg/kg/day) in sterile PBS containing 0.1 % BSA were given in two half doses of (1.5 and 5 ug /kg/day respectively, via an intraperitoneal route (ip) for 14 days. Treatments were administered from the onset of disease for 14 days and then were stopped. No insulin therapy was administered during this study.

Measurement of each of plasma C-peptide level and pancreatic insulin content were 75 conducted for the subjects in each treatment group. Analysis of the data shows that, while treatment with EGF and gastrin caused increases both of plasma C-peptide and pancreatic insulin levels significantly greater than these levels in the vehicle treated group or the

Sirolimus/Tacrolimus treated group, treatment with the combination of EGF and gastrin and

Sirolimus and Tacrolimus was most effective in increasing both the plasma C-peptide level and pancreatic insulin content in these NOD mice after diabetes onset. See Figure 4.

Claims

What is claimed is:

I. A method for manufacture of a medicament for use in treating a diabetic subject, the medicament comprising a composition for islet neogenesis therapy and an agent for suppressing an immune response.
2. The method according to claim 1, wherein the composition for islet neogenesis therapy comprises an EGF receptor ligand.
3. A method according to claim 1, further comprising at least two agents for suppressing an immune response.
4. The method according to any of claims 1-3, wherein the composition for islet neogenesis therapy comprises a gastrin/cholecystekinin (CCK) receptor ligand.
5. The method according to claim 2, wherein the EGF receptor ligand is a recombinant human EGF.
6. The method according to claim 2, wherein the EGF receptor ligand is EGF51N.
7. The method according to claim 4, wherein the gastrin/CCK receptor ligand is human gastrinl7.
8. The method according to claim 1, wherein the agent for suppressing immune response is a drug.
9. The method according to claim 1, wherein the agent for suppressing immune response is at least one selected from of the group consisting of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6-mercaptopurine; FK506 (Tacrolimus); 15-deoxyspergualin; an FTY 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4-octylphenyl)ethyl]propane-

1,3-diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; and a demethimmunomycin.
10. The method according to claim 9, wherein the rapamycin is Everolimus or Sirolimus.
11. The method according to claim 9, wherein the corticosteroid is dexamethasone.
12. The method according to claim 1, wherein the agent for suppressing immune response is a protein.
13. The method according to claim 12, wherein the protein comprises an amino acid sequence of an antibody.
14. The method according to claim 1, wherein the agent for suppressing immune response is at least one selected from the group consisting of: hul 124; BTI-322; allotrap- HLA-B270; OKT4A; Enlimomab; ABX-CBL; OKT3; ATGAM,; basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247; Medi-500; Medi-507; Alefacept; efalizumab; infliximab; and an interferon.
15. The method according to claim 1, wherein in manufacture of the medicament, the islet neogenesis therapy composition and the agent for suppressing immune response are formulated for sequential administration.
16. The method according to claim 15 wherein in manufacture of the medicament, the composition and the agent are formulated for storage during a period of time of at least one day between administering the agent and administering the composition.
17. The method according to claim 15 wherein in manufacture of the medicament, the composition and the agent are formulated for storage during a period of time of at least one week between administering the agent and administering the composition.
18. The method according to claim 15 wherein in manufacture of the medicament, the composition and the agent are formulated for storage during a period of time of at least six weeks between administering the agent and administering the composition.
19. The method according to claim 1, wherein in manufacture of the medicament, at least one of the islet neogenesis therapy composition and the agent for suppressing immune response are formulated for systemic administration.
20. The method according to claim 19, wherein in manufacture of the medicament, at least one of the islet neogenesis therapy composition and the agent for suppressing immune response are formulated for bolus administration.
21. The method according to claim 1, wherein in manufacture of the medicament, at least one of the islet neogenesis therapy composition and the agent for suppressing immune response are formulated for administration by a route selected from the group consisting of intravenous, subcutaneous, intraperitoneal, and intramuscular.
22. The method according to claim 1, wherein in manufacture of the medicament, at least one of the islet neogenesis therapy composition and the agent for suppressing immune response are formulated for oral administration.
23. The method according to claim 1, wherein in manufacture of the medicament, the agent for suppressing immune response is formulated to contain at least one selected from the group of Sirolimus, Tacrolimus, Everolimus, ISAtx247, and daclizumab.
24, The method according to claim 1, wherein in manufacture of the medicament, the agent for suppressing immune response is formulated for administration to a diabetic mammal. Amended sheet: 20 January 2006
25. The method according to claim 1, wherein in manufacture of the medicament, the agent for suppressing immune response is formulated for administration to a human.
26. A method for manufacture of a medicament for use in treating a diabetic subject, the medicament comprising a composition for islet neogenesis therapy consisting of at least one of an EGF receptor ligand and a gastrin/CCK receptor ligand, and at least one immunosuppressing agent.
27. The method according to claim 26, wherein the medicament is formulated to comprise the gastrin/CCK receptor ligand and the at least one immunosuppressing agent in the absence of the EGF receptor ligand.
28. The method according to claim 27, wherein the medicament is formulated to administer the gastrin/CCK receptor ligand and the at least one immunosuppressing agent in the absence of the EGF receptor ligand, and the method further comprises a period of no administration of any of the agent or the composition.
29. The method according to claim 26, wherein the medicament is formulated for a diabetic subject having recent onset diabetes.
30. The method according to claim 28, wherein the medicament is formulated for sequential administration of the composition and the agent.
31. The method of claim 26, wherein the medicament is formulated so that each of the EGF receptor ligand, the gastrin/CCK receptor ligand, and the immunosuppressing agent are in an effective dose.
32. The method of any of claims 1 and 26, wherein the composition or agent is further formulated to comprise a pharmaceutically acceptable buffer. Amended sheet: 20 January 2006

) . . : Co cL : Cee CE

:
33. A method for manufacture of a medicament for use in treating a diabetic subject, the medicament comprising a composition for islet neogenesis therapy consisting of at least one of EGF51N and gastrinl7, and at least one immunosuppressing agent.
34. A method according to claim 33, wherein the medicament is formulated so that the agent is an effective dose of each of Tacrolimus and Sirolimus.
35. A method according to claim 33, wherein the medicament is formulated to comprise an effective dose of at least one of EGFSIN and gastrin 7, and an effective dose of at least one of Tacrolimus, Everolimus, daclizumab, ISAtx247, and Sirolimus.
36. A method for manufacture of a medicament for use in treating a diabetic subject, the medicament comprising a gastrin/CCK receptor ligand and an agent for suppressing an immune response.
37. The method according to claim 36, wherein the gastrin/CCK receptor ligand is gastrinl7.
38. The method according to claim 36, wherein the agent for suppressing immune response is a drug.
39. The method according to claim 36, wherein the agent for suppressing immune response is selected from at least one of the group consisting of a rapamycin; a corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6-mercaptopurine; an FK506; an ISAtx247; a 15-deoxyspergualin; an FTY : 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4- octylphenyl)ethyl]propane-1,3-diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; and a demethimmunomycin.
40. The method according to claim 39, wherein the rapamycin is Sirolimus or Everolimus.
41. The method according to claim 39, wherein the corticosteroid is dexamethasone.
42. The method according to claim 36, wherein the agent for suppressing immune response is a protein.
43. The method according to claim 42, wherein the protein comprises an amino acid sequence of an antibody.
44. The method according to claim 43, wherein the agent for suppressing immune response is at least one selected from the group consisting of: hul 124; BTI-322; allotrap- HLA-B270; OKT4A; Enlimomab; ABX-CBL; OKT3; ATGAM,; basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247; Medi-500; Medi-507; Alefacept; efalizumab; and infliximab.
45. The method according to claim 36, wherein the gastrin/CCK receptor ligand and the agent for suppressing immune response are administered sequentially.
46. The method according to claim 36, wherein the gastrin/CCK receptor ligand is formulated for bolus administration.
47. The method according to claim 36, wherein the receptor ligand is formulated for administration by a route selected from the group consisting of intravenous, subcutaneous, intraperitoneal, and intramuscular.
48. The method according to claim 36, wherein the agent for suppressing immune response is formulated for administration by a route selected from the group consisting of an oral, systemic, intravenous, subcutancous, intraperitoneal, and intramuscular routes of : delivery.
49. The method according to claim 36, wherein the agent for suppressing immune response is selected from at least one of Tacrolimus, [SAtx247, Everolimus, Sirolimus, and daclizumab.
50. The method according to any of claims 1, 26, 33, and 36, further comprising measuring a physiological parameter in the subject selected from the group of measuring level of: fasting blood glucose; pancreatic insulin content; pancreatic p cell content; and plasma insulin C peptide.
51. The method according to claim 36, wherein the medicament is formulated for a subject that is a mammal.
52. The method according to claim 36, wherein the medicament is formulated for a subject that is a diabetic mammal.
53. The method according to claim 36, wherein the medicament is formulated for a subject that is a diabetic mammal with recent onset diabetes.
54. The method according to claim 36, wherein the medicament is formulated for a subject that is a human.
55. A method for manufacture of a medicament for use in treating a diabetic subject, the medicament having a composition comprising an effective dose of gastrin17 and an effective ‘ dose of at least one immunosuppressing agent.
56. A method according to claim 55, wherein the at least one immunosuppressing agent is Tacrolimus or Sirolimus. FRU PE FE - kd
57. A method according to claim 56, the medicament further comprising an effective dose of ISAtx247 or daclizumab.
58. The method according to any of claims 55-57, wherein the medicament further includes a pharmaceutically acceptable buffer.
59. A pharmaceutical composition comprising an agent for suppressing an immune response and at least one of an EGF receptor ligand and a gastrin/CCK receptor ligand.
60. The composition according to claim 59, wherein the gastrin/CCK receptor ligand is a gastrin.
61. The composition according to claim 59, wherein the gastrin/CCK receptor ligand is a gastrin 17.
62. The composition according to claim 61, wherein the gastrinl7 is gastrinl7Metl5 or gastrinl7Leul5.
63. The composition according to claim 59, wherein the EGF receptor ligand is EGF.
64. The composition according to claim 59, wherein the EGF is recombinant human EGFS1N.
65. The composition according to claim 59, wherein the agent for suppressing immune response is a drug.
66. The composition according to claim 59, wherein the agent for suppressing immune response is at least one selected from of the group consisting of a rapamycin ; a - Amended sheet: 20 January 2006 corticosteroid; an azathioprine; mycophenolate mofetil; a cyclosporine; a cyclophosphamide; a methotrexate; a 6-mercaptopurine; FK506 (Tacrolimus); 15-deoxyspergualin; an FTY 720; a mitoxantrone; a 2-amino-1,3-propanediol; a 2-amino-2[2-(4-octylphenyl)ethyl]propane- 1,3-diol hydrochloride; a 6-(3-dimethyl-aminopropionyl) forskolin; and a demethimmunomycin.
67. The composition according to claim 59, wherein the agent for suppressing immune response is a protein.
68. The composition according to claim 59, wherein the agent for suppressing immune response is a portion of an antibody.
69. The composition according to claim 59, wherein the agent for suppressing immune response is at least one selected from the group consisting of: hul 124; BTI-322; allotrap- HLA-B270; OKT4A; Enlimomab; ABX-CBL; OKT3; ATGAM, basiliximab; daclizumab; antithymocyte immunoglobulin; ISAtx247; Medi-500; Medi-507; Alefacept; efalizumab; infliximab; and an interferon.
70. A kit for treatment of a diabetic subject, comprising a composition for islet neogenesis therapy, an immunosuppressive agent, and a container.
71. A kit comprising at least one dose of a composition according to any of claims 59-69.
72. AKkit according to any of claims 70-72, further comprising instructions for use.
73. A method of manufacture of a medicament for use in treating a diabetic subject, the method comprising manufacture of a composition comprising an effective dose of gastrinl7 and an effective dose of at least one immunosuppressing agent.
74. A method of manufacture of a medicament for use in treating a diabetic subject, the method comprising manufacture of a medicament comprising an effective dose of gastrinl7 and an effective dose of each of Tacrolimus and Sirolimus.
75. Use of an effective dose of each of gastrinl7 and at least one immunosuppressing agent in manufacture of a composition for treating a diabetic subject.
76. Use of an effective dose of gastrinl7 and an effective dose of each of Tacrolimus and Sirolimus in manufacture of a medicament for treating a diabetic subject.
77. Use of an immunosuppressing agent and at least one of an EGF receptor ligand and a gastrin/CCK receptor ligand for the manufacture of a medicament for treating a diabetic subject.
78. A method for manufacture of a medicament for use in treating a diabetic subject, the medicament comprising a composition for islet neogenesis therapy and an agent for suppressing an immune response, wherein the composition comprises at least one of a gastrin and an EGF or active analogs or fragments thereof, and the agent is a rapamycin and/or FK506 (Tacrolimus).
79. A method according to claim 78 wherein the rapamycin is Everolimus or Sirolimus.
80. A method according to claim 78 wherein the agent comprises Sirolimus and FK506 (Tacrolimus).
81. A method according to claim 78 or 79 wherein in manufacture of the medicament the composition for islet neogenesis therapy and the agent are formulated for sequential administration.
82. A method according to any one of claims 78 to 81 wherein in manufacture of the medicament the composition and the agent are formulated for oral administration. Amended sheet: 20 January 2006
83. A method according to any one of claims 78 to 82 wherein the medicament is formulated to comprise a gastrin or active analogs or fragments thereof, Sirolimus, and FK506 (Tacrolimus).
84 A method according to any one of claims 78 to 83 wherein the medicament is formulated to administer the gastrin and agent in the absence of EGF, and the method further comprises a period of no administration of the agent or the gastrin.
85. A method according to any one of claims 78 to 84 wherein the medicament is formulated so that each of the composition and the agent are in an effective dose to increase plasma C-peptide levels and pancreatic insulin content.
86. A method according to claim 78 wherein the medicament comprises a composition for islet neogenesis therapy consisting of at least one of EGF51N and gastrinl7, and at least one immunosuppressing agent, wherein the immunosuppressing agent is rapamycin and/or FK506 (Tacrolimus).
87. A method according to claim 86 wherein the medicament is formulated so that the agent is an effective dose of each of Tacrolimus and Sirolimus.
88. A method according to claim 86 wherein the medicament is formulated to comprise an effective dose of at least one of EGF51N and gastrinl7, and an effective dose of at least one of Tacrolimus and Sirolimus.
89. A method according to claim 86 or 88 wherein the rapamycin is Sirolimus.
90. A method according to any one of claims 78 to 89 comprising measuring a physiological parameter in the subject selected from the group of measuring levels of: fasting blood glucose; pancreatic insulin content; pancreatic pf cell content; and plasma insulin C peptide. : Amended sheet: 20 January 2006
91. A method according to any one of claims 78 to 90 wherein the medicament is formulated for a subject that is a mammal.
92. A method according to any one of claims 78 to 91 wherein the medicament is formulated for a subject that is a diabetic mammal. :
93. A method according to any one of claims 78 to 92 wherein the medicament is formulated for a subject that is a diabetic mammal with recent onset diabetes.
94. A method according to any one of claims 78 to 93 wherein the medicament is formulated for a subject that is human.
95. A pharmaceutical composition comprising an agent for suppressing an immune response and at least one of EGF or a gastrin/CCK receptor ligand wherein the agent is at least one of Tacrolimus and rapamycin.
96. A pharmaceutical composition according to claim 95 wherein the gastrin/CCK receptor ligand is a gastrin.
97. A pharmaceutical composition according to claim 96 wherein the gastrin is gastrin

17.
98. A pharmaceutical composition according to claim 97 wherein the gastrinl7 is gastrin] 7Met15 or gastrinl7LeulS5.
99. A pharmaceutical composition according to claim 95, 96, 97, or 98 wherein the EGF is recombinant human EGF51N.
100. A kit for treatment of a diabetic subject comprising a composition for islet neogenesis therapy as claimed in one of claims 95 to 99, an immunosuppressive agent, and a container, wherein the immunosuppressive agent is at least one of Tacrolimus and rapamycin. Amended sheet: 20 January 2006
101. A pharmaceutical composition according to claim 59, substantially as herein described with reference to any one of the illustrative examples.
102. A pharmaceutical composition according to claim 95, substantially as herein described with reference to any one of the illustrative examples. Amended sheet: 20 January 2006 I pr REF se ae wi an . ) oo SLB LL N ul