ZA200308192B - Anti-inflammatory 17.beta.-carbothioate ester derivatives of androstane with a cyclic ester group in position 17.alpha. - Google Patents

Description

ANTI-INFLAMMATORY 17.BETA.-CARBOTHIOATE ESTER DERIVATIVES OF ANDROSTANE

WITH A CYCLIC ESTER GROUP IN POSITION 17.ALPHA

The present invention relates to novel anti-inflammatory and anti-allergic

R compounds of the androstane series and to processes for their preparation. The present invention also relates to pharmaceutical formulations containing the , compounds and to therapeutic uses thereof, particularly for the treatment of inflammatory and allergic conditions.

Glucocorticoids which have anti-inflammatory properties are known and are widely used for the treatment of inflammatory disorders or diseases such as asthma and rhinitis. For example, US Patent 4335121 discloses 6a, 9a-Difluoro-170-(1- oxopropoxy)-11p-hydroxy-16a-methyl-3-oxo-androsta-1,4-diene-17p-carbothioic acid

S-fluoromethyl ester (known by the generic name of fluticasone propionate) and derivatives thereof. The use of glucocorticoids generally, and especially in children, has been limited in some quarters by concerns over potential side effects. The side effects that are feared with glucocorticoids include suppression of the Hypothalamic-

Pituitary-Adrenal (HPA) axis, effects on bone growth in children and on bone density in the elderly, ocular complications (cataract formation and glaucoma) and skin atrophy. Certain glucocorticoid compounds also have complex paths of metabolism wherein the production of active metabolites may make the pharmacodynamics and pharmacokinetics of such compounds difficult to understand. Whilst the modem steroids are very much safer than those originally introduced it remains an object of ol research to produce new molecules which have excellent anti-inflammatory properties, with predictable pharmacokinetic and pharmacodynamic properties, with an attractive side effect profile, and with a convenient treatment regime.

Certain novel androstane derivatives are disclosed in W002/12265 and

WO02/12266 (Glaxo Group), both of these documents being published after the earliest priority date of this patent application.

We have identified a novel series of glucocorticoids, which substantially meets these objectives.

Thus, according to one aspect of the invention, there is provided a compound of formula (1)

RECTIFIED SHEET (RULE 91) ISAJEP

0 s” 0]

HO4 Te cog ea R, 0) fo 00 '

Re wherein

R, represents C, alkyl or C, 4 haloalkyl;

R; represents C,; cycloalkyl or C,, cycloalkenyl either of which may optionally be substituted by one or more groups selected from oxo, methyl, methylene and halogen;

R, represents hydrogen, methyl (which may be in either the a or B configuration) or methylene; :

R, and Rg are the same or different and each represents hydrogen or halogen; and “== represents a single or a double bond; and solvates thereof.

Examples of solvates include hydrates.

References hereinafter to a compound according to the invention includes both compounds of formula (I) and solvates thereof.

It will be appreciated that the invention includes within its scope all stereoisomers (including enantiomers and diastereoisomers) of the compounds of formula (I) and mixtures thereof.

Preferably, the absolute stereochemistry will be as shown in the representation of compounds of formula (1).

Examples of C, haloalkyl! that R, may represent include C, alkyl substituted by 1-3 halogen atoms, preferably 1 halogen atom. Preferred halogen atoms are selected from bromine, chlorine and fluorine. .

Examples of C,¢ cycloalkyl groups that R, may represent include cyclopropyi, cyclobutyl, cyclopentyl and cyclohexyl and substituted derivatives such as : methylcyclopropyl (eg 1-methyicyclopropyl or 2-methylcyclopropyl), dichlorocyclopropyl (eg 2,2-dichloropropyl), methyldichlorocyclopropyl (eg 1-methyl- 2,2-dichlorocyclopropyl), exomethylenecyclobutyl (eg 3-exomethylenecyclobutyl),

tetramethylcyclopropyl (eg 2,2,3,3- tetramethylicyclopropyl) and methycyclobutyl (eg 1-methylcyclobutyl). Other examples include dimethylcyclobutyl (eg 3,3- dimethylcyclobutyl), difluorocyclobutyl (eg 3,3-difluorocyclobutyl), methylcyclopentyl . (eg 1-methyicyclopentyl). A further example includes oxocyclobutyl (eg 3-oxo- 5S cyclobutyl). ' Examples of C,4 cycloalkenyl groups that R, may represent include alkenyl groups containing 1 or more double bonds (not being aromatic groups) such as cyclohexenyl eg cyclohex-2,3-enyl.

We prefer R, to represent fluoromethyl, chloromethyl, bromomethyl or 2’- fluoroethyl, especially fluoromethyl.

Preferably, R, represents C;; cycloalkyl or C;; cycloalkenyl either of which may optionally be substituted by one or more groups selected from methyl, methylene and halogen. In an alternative aspect, R, represents C,; cycloalkyl or C3 cycloalkenyl either of which may be substituted by oxo eg 3-oxocyclobutyl.

We prefer R, to represent C, ; cycloalkyl optionally substituted by one or more methyl and/or halogen groups. We particularly prefer R, to represent C,4 cycloalkyl, more preferably C4 cycloalkyl, optionally substituted by one or more methyl or chlorine groups.

We also prefer R, to represent C, ; cycloalkyl substituted by methylene.

In one set of preferred compounds, R, is unsubstituted or substituted by at most one methyl or chlorine group. More preferably, R, is substituted by one methyl group, especially in the 1-position, eg 1-methyl cyclopropyl or 1-methyl-cyclobutyl.

In another set of preferred compounds, R, is substituted by more than one methyl group, eg 2,2,3,3- tetramethylcyclopropyl.

We prefer R; to represent methyl, especially methyl in the a configuration.

Compounds of formula (I) in which R, and R;, which can be the same or different, each represents hydrogen, fluorine or chlorine, particularly hydrogen or fluorine, are preferred. Especially preferred are compounds in which both R, and Rs are fluorine. : 30 Preferably, = represents a double bond.

It is to be understood that the present invention covers all combinations of ) particularly and preferred groups referred to hereinabove.

Preferred compounds of formula (1) include: 17 a~(Cyclobutylcarbonyl)oxy-6a,9a-difluoro-118-hydroxy-16a-methyl-3-oxo- androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester,

17a~(Cyclopentylcarbonyl)oxy- 6a, Sa-difluoro-11p-hydroxy-16a-methyl-3-oxo- androsta-1,4-diene-178-carbothioic acid S-fluoromethy! ester; 17a~(Cyclohexylcarbonyl)oxy- 6a,9a-difluoro-11p-hydroxy-16a-methyl-3-oxo- androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; ' 17u-(Cyclopropylcarbonyl)oxy- 6a, 9a-difluoro-11p-hydroxy-16a~-methyl-3-oxo- androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; 6a, 9a-Difluoro-11p-hydroxy-16a-methyl-17a-(1-methycyclopropylcarbonyl) oxy-3-oxo-androsta-1,4-diene-17-carbothioic acid S-fluoromethy! ester; 6a,9a-Difluoro-11pB-hydroxy-16a-methyl-3-oxo-17a-(2,2,3,3- tetramethycyclopropylcarbonyi)oxy-androsta-1,4-diene-17B-carbothioic acid S- fluoromethy! ester; 17a-(2,2-Dichloro-1-methycyclopropylcarbonyl)oxy-6a., 9a-difluoro-11p-hydroxy-16a- methyl-3-oxo-androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester; 17a~(2,2-Dichlorocylopropylcarbonyl)oxy-6a,9a-difluoro-11p-hydroxy-16o-methyl-3- oxo-androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; 6a, 9a-Difluoro-11B-hydroxy-16a-methyl-17a-(3-methylenecyclobutylcarbonyl)oxy-3- oxo-androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester; 6a,9a-Difluoro-11B-hydroxy-16a-methyl-17a-(2-methylcyclopropylcarbony!)oxy-3- oxo-androsta-1,4-diene-173-carbothioic acid S-fluoromethyl ester; 6a,9a-Difluoro-11p-hydroxy-16a-methyl-17 a-(1-methylcyclobutylcarbonyl)oxy-3-oxo- androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; 6a,9a-Difluoro-17a-(3,3-dimethylcyclobutylcarbonyl)oxy-11B-hydroxy-16a-methyl-3- ] oxo-androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; 6a, 9a-Difluoro-17a~(3,3-difluorocyclobutylcarbonyljoxy-113-hydroxy-16a-methyl-3- oxo-androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; 6a,9a-Difluoro-11B-hydroxy-16a-methyl-17a-(1-methylcyclopentylcarbonyl)oxy-3- oxo-androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester; 6c, 9a-Difluoro-11p-hydroxy-16a-methyl-3-oxo-17a-(3-oxocyclobutylcarbonyl)oxy- androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester.

One particularly preferred compound is the following: , 6c, 9a-Difluoro-11p-hydroxy-16a-methyl-17a-(1-methycyclopropylcarbonyl) oxy-3-oxo-androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester.

Another particularly preferred compound is the following:

6a, 9a-Difluoro-11p-hydroxy-16a-methyl-3-oxo-170-(2,2,3,3- tetramethycyclopropylcarbonyl)oxy-androsta-1,4-diene-17p-carbothioic acid S- fluoromethyl ester. : Another particularly preferred compound is the following: 5 6a,9a-Difluoro-11p-hydroxy-16a-methyl-17a-(1-methylcyclobutylcarbonyl)oxy-3-oxo-

A androsta-1,4-diene-178-carbothioic acid S-fluoromethy! ester.

The compounds of formula (I) have potentially beneficial anti-inflammatory or anti-allergic effects, particularly upon topical administration, demonstrated by, for example, their ability to bind to the glucocorticoid receptor and to illicit a response via that receptor with long lasting effect. Hence, the compounds of formula (1) are useful in the treatment of inflammatory and/or allergic disorders, especially in once-per-day therapy.

Examples of disease states in which the compounds of the invention have utility include skin diseases such as eczema, psoriasis, allergic dermatitis neurodermatitis, pruritis and hypersensitivity reactions; inflammatory conditions of the nose, throat or lungs such as asthma (including allergen-induced asthmatic reactions), rhinitis (including hayfever), nasal polyps, chronic obstructive pulmonary disease, interstitial lung disease, and fibrosis; inflammatory bowel conditions such as ulcerative colitis and Crohn's disease; and auto-immune diseases such as rheumatoid arthritis.

Compounds of the invention may also have use in the treatment of conjunctiva and conjunctivitis.

It will be appreciated by those skilled in the art that reference herein to treatment extends to prophylaxis as well as the treatment of established conditions.

As mentioned above, compounds of formula (I) are useful in human or veterinary medicine, in particular as anti-inflammatory and anti-allergic agents, especially in once-per-day therapy.

There is thus provided as a further aspect of the invention a compound of formula (I) or a physiologically acceptable solvate thereof for use in human or ’ 30 veterinary medicine, particularly in the treatment of patients with inflammatory and/or allergic conditions. Pharmaceutical compositions for once-per-day administration are ot of particular interest.

According to another aspect of the invention, there is provided the use of a compound of formula (I) or physiologically acceptable solvate thereof for the manufacture of a medicament for the treatment of patients with inflammatory and/or allergic conditions, especially for therapy once-per-day.

In a further or alternative aspect, there is provided a method for the treatment of a human or animal subject with an inflammatory and/or allergic condition, which ) method comprises administering to said human or animal subject an effective amount of a compound of formula (1) or physiologically acceptable solvate thereof, especially administration once-per-day.

The compounds according to the invention may be formulated for administration in any convenient way, and the invention therefore also includes within its scope pharmaceutical compositions comprising a compound of formula (I) or physiologically acceptable solvate thereof together, if desirable, in admixture with one or more physiologically acceptable diluents or carriers.

Further, there is provided a process for the preparation of such pharmaceutical compositions which comprises mixing the ingredients.

The compounds according to the invention may, for example, be formulated for oral, buccal, sublingual, parenteral, local or rectal administration, especially local administration.

Local administration as used herein, includes administration by insufflation and inhalation. Examples of various types of preparation for local administration include ointments, lotions, creams, gels, foams, preparations for delivery by transdermal patches, powders, sprays, aerosols, capsules or cartridges for use in an inhaler or insufflator or drops (e.g. eye or nose drops), solutions/suspensions for nebulisation, suppositories, pessaries, retention enemas and chewable or suckable tablets or pellets (e.g. for the treatment of aphthous ulcers) or liposome or microencapsulation preparations.

Advantageously compositions for topical administration to the lung include dry : powder compositions and spray compositions.

Dry powder compositions for topical delivery to the lung may, for.example, be presented in capsules and cartridges for use in an inhaler or insufflator of, for example, gelatine. Formulations generally contain a powder mix for inhalation of the . compound of the invention and a suitable powder base such as lactose or starch.

Use of lactose is preferred. Each capsule or cartridge may generally contain ’ between 20pg-10mg of the compound of formula (1) optionally in combination with another active ingredient. Alternatively, the compound of the invention may be presented without excipients. Packaging of the formulation may be suitable for unit dose or multi-dose delivery. In the case of multi-dose delivery, the formulation can be pre-metered (eg as in Diskus, see GB 2242134 or Diskhaler, see GB 2178965, 2129691 and 2169265) or metered in use (eg as in Turbuhaler, see EP 69715). An . ' example of a unit-dose device is Rotahaler (see GB 2064336). The Diskus inhalation device comprises an elongate strip formed from a base sheet having a plurality of ¢ recesses spaced along its length and a lid sheet hermetically but peelably sealed thereto to define a plurality of containers, each container having therein an inhalable formulation containing a compound of formula (I) preferably combined with lactose.

Preferably, the strip is sufficiently flexible to be wound into a roll. The lid sheet and base sheet will preferably have leading end portions which are not sealed to one another and at least one of the said leading end portions is constructed to be attached to a winding means. Also, preferably the hermetic seal between the base and lid sheets extends over their whole width. The lid sheet may preferably be peeled from the base sheet in a longitudinal direction from a first end of the said base sheet. :

Pharmaceutical formulations which are non-pressurised and adapted to be administered as a dry powder topically to the lung via the buccal cavity (especially those which are free of excipient or are formulated with a diluent or carrier such as lactose or starch, most especially lactose) are of particular interest.

Spray compositions may for example be formulated as aqueous solutions or suspensions or as aerosols delivered from pressurised packs, such as a metered dose inhaler, with the use of a suitable liquefied propellant. Aerosol compositions suitable for inhalation can be either a suspension or a solution and generally contain the compound of formula (I) and a suitable propellant such as a fluorocarbon or hydrogen-containing chlorofluorocarbon or mixtures thereof, particularly hydrofluoroalkanes, especially 1,1,1,2-tetrafluoroethane, 1,1,1,2,3,3,3-heptafluoro-n- propane or a mixture thereof. The aerosol composition may optionally contain additional formulation excipients well known in the art such as surfactants eg oleic acid or lecithin and cosolvents eg ethanol. One example formulation is excipient free . 30 and consists essentially of (eg consists of) compound of formula (I) (preferably in unsolvated form eg as Form 1) (optionally in combination with another therapeutically . active ingredient) and a propellant selected from 1,1,1,2-tetrafluoroethane, 1,1,1,2,3,3,3-heptafluoro-n-propane and mixture thereof. Another example formulation comprises particulate compound of formula (I), a propellant selected from 1,1,1,2-tetrafluoroethane, 1,1,1,2,3,3,3-heptafluoro-n-propane and mixture thereof and a suspending agent which is soluble in the propellant eg an oligolactic acid or derivative thereof as described in W094/21229. The preferred propellant is 1,1,1,2- tetrafluoroethane. As noted elsewhere in this specification, compound of formula {)) does not appear to form a solvate with 1,1,1,2-tetraflucroethane. Pressurised formulations will generally be retained in a canister (eg an aluminium canister) closed with a valve (eg a metering valve) and fitted into an actuator provided with a ) mouthpiece.

Medicaments for administration by inhalation desirably have a controlled particle size. The optimum particle size for inhalation into the bronchial system is usually 1-10um, preferably 2-5um. Particles having a size above 20um are generally too large when inhaled to reach the small airways. To achieve these particle sizes the particles of compound of formula (I) as produced may be size reduced by conventional means eg by micronisation. The desired fraction may be separated out by air classification or sieving. Preferably, the particles will be crystalline, prepared for example by a process which comprises mixing in a continuous flow cell in the “presence of ultrasonic radiation a flowing solution of compound of formula (1) as medicament in a liquid solvent with a flowing liquid antisolvent for said medicament (eg as described in International Patent Application PCT/GB99/04368) or else by a process which comprises admitting a stream of solution of the substance in a liquid solvent and a stream of liquid antisolvent for said substance tangentially into a cylindrical mixing chamber having an axial outlet port such that said streams are thereby intimately mixed through formation of a vortex and precipitation of crystalline particles of the substance is thereby caused (eg as described in International Patent

Application PCT/GB00/04327). When an excipient such as lactose is emnloyed. generally, the particle size of the excipient will be much greater than the inhaled medicament within the present invention. When the excipient is lactose it will typically be present as milled lactose, wherein not more than 85% of lactose particles will have a MMD of 60-90um and not less than 15% will have a MMD of less than 15um.

Formulations for administration topically to the nose (eg for the treatment of rhinitis) include pressurised aerosol formulations and aqueous formulations administered to the nose by pressurised pump. Formulations which are non- pressurised and adapted to be administered topically to the nasal cavity are of ' particular interest. The formulation preferably contains water as the diluent or carrier for this purpose. Aqueous formulations for administration to the lung or nose may be provided with conventional excipients such as buffering agents, tonicity modifying agents and the like. Aqueous formulations may also be administered to the nose by nebulisation.

Other possible presentations include the following: . Ointments, creams and gels, may, for example, be formulated with an aqueous or oily base with the addition of suitable thickening and/or gelling agent * and/or solvents. Such bases may thus, for example, include water and/or an oil such as liquid paraffin or a vegetable oil such as arachis oil or castor oil, or a solvent such as polyethylene glycol. Thickening agents and gelling agents which may be used according to the nature of the base include soft paraffin, aluminium stearate, cetostearyl alcohol, polyethylene glycols, woolfat, beeswax, carboxypolymethylene and cellulose derivatives, and/or glyceryl monostearate and/or non-ionic emulsifying agents.

Lotions may be formulated with an aqueous or oily base and will in general also contain one or more emulsifying agents, stabilising agents, dispersing agents, suspending agents or thickening agents.

Powders for external application may be formed with the aid of any suitable powder base, for example, talc, lactose or starch. Drops may be formulated with an aqueous or non-aqueous base also comprising one or more dispersing agents, solubilising agents, suspending agents or preservatives.

Advantageously, the formulations of the invention may be buffered by the addition of suitable buffering agents.

The proportion of the active compound of formula (I) in the local compositions according to the invention depends on the precise type of formulation to be prepared but will generally be within the range of from 0.001 to 10% by weight. Generally, however for most types of preparations advantageously the proportion used will be within the range of from 0.005 to 1% and preferably 0.01 to 0.5%. However, in powders for inhalation or insufflation the proportion used will be within the range of from 0.1 to 5%.

Aerosol formulations are preferably arranged so that each metered dose or . 30 “puff” of aerosol contains 20pg-2000png, preferably about 20pug-500pg of a compound of formula (I). Administration may be once daily or several times daily, for example 2, , 3, 4 or 8 times, giving for example 1, 2 or 3 doses each time. The overall daily dose with an aerosol will be within the range 100ug-10mg preferably, 200ug-2000ug. The overall daily dose and the metered dose delivered by capsules and cartridges in an inhaler or insufflator will generally be double those with aerosol formulations.

Topical preparations may be administered by one or more applications per day to the affected area; over skin areas occlusive dressings may advantageously be used. Continuous or prolonged delivery may be achieved by an adhesive reservoir system. '

For internal administration the compounds according to the invention may, for example, be formulated in conventional manner for oral, parenteral or rectal ’ administration. Formulations for oral administration include syrups, elixirs, powders, granules, tablets and capsules which typically contain conventional excipients such as binding agents, fillers, lubricants, disintegrants, wetting agents, suspending agents, emulsifying agents, preservatives, buffer salts, flavouring, colouring and/or sweetening agents as appropriate. Dosage unit forms are, however, preferred as described below.

Preferred forms of preparation for internal administration are dosage unit forms i.e. tablets and capsules. Such dosage unit forms contain from 0.1mg to 20mg preferably from 2.5 to 10mg of the compounds of the invention.

The compounds according to the invention may in general may be given by internal administration in cases where systemic adreno-cortical therapy is indicated.

In general terms preparations, for internal administration may contain from 0.05 to 10% of the active ingredient dependent upon the type of preparation involved.

The daily dose may vary from 0.1mg to 60mg, e.g. 5-30mg, dependent on the condition being treated, and the duration of treatment desired.

Slow release or enteric coated formulations may be advantageous, particularly for the treatment of inflammatory bowel disorders.

The pharmaceutical compositions according to the invention may also be used in combination with another therapeutically active agent, for example, aj, adrenoreceptor agonist, an anti-histamine or an anti-allergic. The invention thus provides, in a further aspect, a combination comprising a compound of formula (1) or a physiologically acceptable solvate thereof together with another therapeutically active agent, for example, a B,-adrenoreceptor agonist, an anti-histamine, an anti- allergic or an anti-cholinergic. )

Examples of p,-adrenoreceptor agonists include salmeterol (eg as xinafoate), salbutamol (eg as sulphate), formoterol (eg as fumarate), fenoterol or terbutaline (eg ' as sulphate). Long-acting B,-adrenoreceptor agonists are preferred, especially those having a therapeutic effect over a 24 hour period.

Especially preferred long-acting B,-adrenoreceptor agonists are compounds of formula (X)

HOCH, R'™

RM

To WT comm) X)

OH

Rr ora salt or solvate thereof, wherein: m is an integer of from 2 to 8; n is an integer of from 3 to 11, preferably from 3 to 7; with the proviso that m + nis 5 to 19, preferably 5 to 12;

R" is =XSO,NR'"R" wherein X is —(CH,),- or C, alkenylene;

R' and R" are independently selected from hydrogen, C, alkyl, C, ;cycloalkyl,

C(O)NR™R", phenyl, and phenyl (C,_alkyl)-, or R™ and RY, together with the nitrogen to which they are bonded, form a 5-, 6-, or 7- membered nitrogen containing ring, and R'® and R" are each optionally substituted by one or two groups selected from halo, C, salkyl, C,¢haloalkyl, C,calkoxy, hydroxy- substituted C, salkoxy, -CO,R", -SO,NR"R", -CONR"R'®, -NR"*C(O)R", or a 5-, 6- or 7-membered heterocylic ring;

Rand R* are independently selected from hydrogen, C,.alkyl,

Cs cycloalkyl, phenyl, and phenyl (C, alkyl)-; and pis an integer of from 0 to 6, preferably from 0 to 4;

R* and R™ are independently selected from hydrogen, C,.calkyl, C, salkoxy, halo, phenyl, and C,chaloalkyl; and

R'" and R* are independently selected from hydrogen and C,_ alkyl with the proviso that the total number of carbon atoms in R' and R* is not more than 4.

In the compounds of formula (1) the group R" is preferably attached to the meta-position relative to the -O-(CH,),- link. ' R" preferably represents —SO,NR'R" wherein R' and R'? are independently selected from hydrogen and C, ¢alkyl, more preferably R' is -SO,NH,.

R" and R* are preferably independently selected from hydrogen and methyl, more preferably R* and R' are both hydrogen. m is suitably 4, 5, or 6, and n is suitably 3, 4, 5 or 6. Preferably mis 5or6and nis 3 or 4, such thatm + nis 8, 9 or 10, preferably 9.

More especially preferred compounds of formula (X) are compounds of formula (Xa)

HOCH,

RM

I WS —0 —en—( F xa) i

OH ora salt or solvate thereof, wherein

R'" is as defined above for formula (X).

Further more especially preferred compounds of formula (X) are compounds of formula (Xb):

HOCH,

RM

I op S— —0 —em— J (Xb)

OH or a salt or solvate thereof, wherein

R" is as defined above for formula (X).

In the compounds of formulae (Xa) and (Xb), the group R"! is preferably attached to the meta-position relative to the -O-(CH,),-, -O-(CH,),- or -O-(CH,):- link respectively. in the compounds of formulae (Xa) and (Xb), R"" is preferably -SO,NR*R*” wherein R'® and RY are independently selected from hydrogen and C, salkyl, more preferably R'! is —SO,NH,.

In the definition of R"! where ‘R* and R" together with the nitrogen atom to which they are bonded, form a 5-, 6-, or 7- membered nitrogen containing ring’, the term “5-, 6-, or 7- membered nitrogen containing ring” means a 5-, 6-, or 7- , membered saturated or unsaturated ring which includes the sulfonamide nitrogen atom and optionally 1 or 2 other heteroatoms independently selected from nitrogen, sulphur, and oxygen. Suitable examples of such a ring include piperidinyl, morpholinyl, and piperaziny!. :

In the definition of R!, specifically the optional substituents on R' and RY, the term “5-, 6-, or 7- membered heterocyclic ring” means a 5-, 6-, or 7- membered fully or partially saturated or unsaturated ring which includes 1, 2, 3 or 4 heteroatoms independently selected from nitrogen, sulphur, and oxygen. Suitable examples of such a ring include pyrrolyl, furyl, thienyl, pyridinyl, pyrazinyl, pyridazinyl, imidazolyl, tetrazolyl, tetrahydrofuranyl, oxazolyl, thiazolyl, thiadiazolyl, piperidinyl, morpholinyl, and piperazinyl. : In the definition of X, the term “alkenylene” includes both cis and trans structures. Suitable examples of alkenylene groups include -CH=CH-. ’ The compounds of formulae (X), (Xa) and (Xb) include an asymmetric centre, namely the carbon atom of the : -CH- or group. The present invention includes both (S) and (R) enantiomers either in substantially pure form or admixed in any proportions.

Similarly, where R" and R* are different groups, the carbon atom to which they are attached is an asymmetric centre and the present invention includes both (S) and (R) enantiomers at this centre either in substantially pure form or admixed in any proportions.

Thus the compounds of formulae (X), (Xa) and (Xb) include all enantiomers and diastereoisomers as well as mixtures thereof in any proportions.

The most preferred compound of formula (X) is 3-(4-{[6-({(2R)-2-Hydroxy-2- [4-hydroxy-3-(hydroxymethyl)phenyl]ethyl}amino)- hexylloxy}butyl)benzenesulfonamide or a salt or solvate thereof.

Salts and solvates of compounds of formulae (X), (Xa) and (Xb) which are suitable for use in medicine are those wherein the counterion or associated solvent is pharmaceutically acceptable. However, salts and solvates having non- pharmaceutically acceptable counterions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of other compounds of formulae (X), (Xa) and (Xb) and their pharmaceutically acceptable salts and solvates.

Suitable salts according to the invention include those formed with both organic and inorganic acids or bases. Pharmaceutically acceptable acid addition salts ' include those formed from hydrochloric, hydrobromic, sulphuric, citric, tartaric, phosphoric, lactic, pyruvic, acetic, trifluoroacetic, triphenylacetic, sulphamic, ’ sulphanilic, succinic, oxalic, fumaric, maleic, malic, glutamic, aspartic, oxaloacetic, methanesulphonic, ethanesulphonic, arylsulphonic (for example p-toluenesulphonic, benzenesulphonic, naphthalenesulphonic or naphthalenedisulphonic), salicylic, glutaric, gluconic, tricarballylic, cinnamic, substituted cinnamic (for example, phenyl,

methyl , methoxy or halo substituted cinnamic, including 4-methyl and 4- methoxycinnamic acid), ascorbic, oleic, haphthoic, hydroxynaphthoic (for example 1- or 3-hydroxy-2-naphthoic), naphthaleneacrylic (for example naphthalene-2-acrylic), benzoic, 4-methoxybenzoic, 2- or 4-hydroxybenzoic, 4-chlorobenzoic, 4- phenylbenzoic, benzeneacrylic (for example 1,4-benzenediacrylic) and isethionic acids. Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases such as dicyclohexyl amine and N-methyl-D-glucamine.

Compounds of formula (X), (Xa) and (Xb) may be prepared by reference to Example

X recited below, by analogous processes, or by other conventional processes known per se.

Since the compounds of formula (I) are long-acting, preferably the composition comprising the compound of formula (I) and the long-acting B,- adrenoreceptor agonists will be delivered once-per-day and the dose of each will be selected so that the composition has a therapeutic effect in the treatment of respiratory disorders effect (eg in the treatment of asthma or COPD, particularly asthma) over 24 hours or more.

Examples of anti-histamines include methapyrilene or loratadine. Examples of anti-allergics include cromoglycate (eg as sodium), ketotifen and nedocromil (as as sodium). Examples of anti-cholinergics include ipratropium (eg as bromide), tiotropium, atropine or oxitropium. Any of the aforementioned substances may be employed in the form of alternative salts or solvates thereof. oo

Other suitable combinations include, for example, other anti-inflammatory agents eg. NSAIDs (eg. PDE4 inhibitors, leukotriene antagonists, iNOS inhibitors, tryptase and elastase inhibitors, beta-2 integrin antagonists and adenosine 2a agonists)) or antiinfective agents (eg. antibiotics, antivirals).

Of particular interest is use of the compounds of formula (1) in combination with a phosphodiesterase 4 (PDE4) inhibitor. The PDE4-specific inhibitor useful in this aspect of the invention may be any compound that is known to inhibit the PDE4 enzyme or which is discovered to act as a PDE4 inhibitor, and which are only PDE4 inhibitors, not compounds which inhibit other members of the PDE family as well as

PDE4. Generally it is preferred to use a PDE4 inhibitor which has an ICs ratio of about 0.1 or greater as regards the ICsq for the PDE4 catalytic form which binds rolipram with a high affinity divided by the ICs for the form which binds rolipram with a low affinity. For the purposes of this disclosure, the cAMP catalytic site which binds

R and S rolipram with a low affinity is denominated the "low affinity" binding site : (LPDE 4) and the other form of this catalytic site which binds rolipram with a high affinity is denominated the “high affinity" binding site (HPDE 4). This term "HPDE4" y should not be confused with the term "hPDE4" which is used to denote human PDE4.

Initial experiments were conducted to establish and validate a [*H]-rolipram binding assay. Details of this work are given in the Binding Assays described in detail below.

The preferred PDE4 inhibitors of use in this invention will be those compounds which have a salutary therapeutic ratio, i.e., compounds which preferentially inhibit cAMP catalytic activity where the enzyme is in the form that binds rolipram with a low affinity, thereby reducing the side effects which apparently are linked to inhibiting the form which binds rolipram with a high affinity. Another way to state this is that the preferred compounds will have an ICs ratio of about 0.1 or greater as regards the IC5q for the PDE4 catalytic form which binds rolipram with a high affinity divided by the IC5q for the form which binds rolipram with a low affinity.

A further refinement of this standard is that of one wherein the PDE4 inhibitor has an

ICsp ratio of about 0.1 or greater; said ratio is the ratio of the ICs value for competing with the binding of 1nM of [3H]R-rolipram to a form of PDE4 which binds rolipram with a high affinity over the IC5g value for inhibiting the PDE4 catalytic activity of a form which binds rolipram with a low affinity using 1uM[3H]-cAMP as the substrate.

Examples of useful PDE4 inhibitors are: (R)-(+)-1-(4-bromobenzyl)-4-[(3-cyclopentyloxy)-4-methoxyphenyl]-2-pyrrolidone; (R)-(+)-1-(4-bromobenzyl)-4-[(3-cyclopentyloxy)-4-methoxyphenyl]-2-pyrrolidone; 3-(cyclopentyloxy-4-methoxyphenyl)-1-(4-N'-[N2-cyano-S-methyl- isothioureido]benzyl)-2-pyrrolidone; cis 4-cyano-4-(3-cyclopentyloxy-4-methoxyphenyl)cyclohexan-1-carboxylic acid]; cis-[4-cyano-4-(3-cyclopropylmethoxy-4-difluoromethoxyphenyl)cyclohexan-1-ol]; , 30 (R)-(+)-ethyl [4-(3-cyclopentyloxy-4-methoxyphenyl)pyrrolidine-2-ylidene]acetate; and (S)-(-)-ethyl [4-(3-cyclopentyloxy-4-methoxyphenyl)pyrrolidine-2-ylidene]acetate. ‘ Most preferred are those PDE4 inhibitors which have an ICs ratio of greater than 0.5, and particularly those compounds having a ratio of greater than 1.0.

Preferred compounds are cis 4-cyano-4-(3-cyclopentyloxy-4- methoxyphenyl)cyclohexan-1-carboxylic acid, 2-carbomethoxy-4-cyano-4-(3-

cyclopropylmethoxy-4-difluoromethoxyphenyl)cyclohexan-1-one and cis-{4-cyano-4- (3-cyclopropylmethoxy-4-difluoromethoxyphenyl)cyclohexan-1-ol]; these are examples of compounds which bind preferentially to the low affinity binding site and which have an ICg( ratio of 0.1 or greater. :

Other compounds of interest include:

Compounds set out in U.S. patent 5,552,438 issued 03 September, 1996; this patent ’ and the compounds it discloses are incorporated herein in full by reference. The compound of particular interest, which is disclosed in U.S. patent 5,552,438, iscis-4- cyano-4-{3- (cyclopentyloxy)-4-methoxyphenylcyclohexane-1-carboxylic acid (also known as cilomalast) and its salts, esters, pro-drugs or physical forms;

AWD-12-281 from Astra (Hofgen, N. et al. 15th EFMC Int Symp Med Chem (Sept 6- 10, Edinburgh) 1998, Abst P.98); a 9-benzyladenine derivative nominated NCS-613 (INSERM); D-4418 from Chiroscience and Schering-Plough; a benzodiazepine PDE4 inhibitor identified as CI-1018 (PD-168787; Parke-Davis/Warner-Lambert); a benzodioxole derivative Kyowa Hakko disclosed in WO 9916766; V-11294A from

Napp (Landells, L.J. et al. Eur Resp J [Annu Cong Eur Resp Soc (Sept 19-23,

Geneva) 1998] 1998, 12(Suppl. 28). Abst P2393); roflumilast (CAS reference No 162401-32-3) and a pthalazinone (WO 9947505) from Byk-Gulden; or a compound identified as T-440 (Tanabe Seiyaku; Fuji, K. et al. J Pharmacol Exp Ther,1998, 284(1): 162).

Phosphodiesterase and Rolipram Binding Assays

Assay method 1A

Isolated human monocyte PDE4 and hrPDE (human recombinant PDE4) was determined to exist primarily in the low affinity form. Hence, the activity of test compounds against the low affinity form of PDE4 can be assessed using standard oo assays for PDE4 catalytic activity employing 1 pM [SH]cAMP as a substrate (Torphy etal, J. of Biol. Chem., Vol. 267, No. 3 pp1798-1804, 1992).

Rat brain high speed supernatants were used as a source of protein and both enantiomers of [3HJ-rolipram were prepared to a specific activity of 25.6 Ci/mmol.

Standard assay conditions were modified from the published procedure to be identical to the PDE assay conditions, except for the last of the CAMP: 50mM Tris

HCI (pH 7.5), 5 mM MgCl,, 50 uM 5'-AMP and 1 nM of [BH}-rolipram (Torphyet al, J. of Biol. Chem., Vol. 267, No. 3 pp1798-1804, 1992). The assay was run for 1 hour at 30° C. The reaction was terminated and bound ligand was separated from free ligand using a Brandel cell harvester. Competition for the high affinity binding site was assessed under conditions that were identical to those used for measuring low affinity PDE activity, expect that [3H]-cAMP was not present.

Assay method 1B : Measurement of Phosphodiesterase Activity

PDE activity was assayed using a [3H]cAMP SPA or [3H]cGMP SPA enzyme assay ' as described by the supplier (Amersham Life Sciences). The reactions were conducted in 96-well plates at room temperature, in 0.1 ml of reaction buffer containing (final concentrations): 50 mM Tris-HCI, pH 7.5, 8.3 mM MgCl,, 1.7 mM

EGTA, [BH]cAMP or [3H] cGMP (approximately 2000 dpm/pmol), enzyme and various concentrations of the inhibitors. The assay was allowed to proceed for 1 hr and was terminated by adding 50 ul of SPA yttrium silicate beads in the presence of zinc sulfate. The plates were shaken and allowed to stand at room temperature for 20 min. Radiolabeled product formation was assessed by scintillation spectrometry. [BH}R-rolipram binding assay

The [BH]R-rolipram binding assay was performed by modification of the method of

Schneider and co-workers, see Nicholson, et al, Trends Pharmacol. Sci., Vol. 12, pp.19-27 (1991) and McHale et al., Mol. Pharmacol., Vol. 39, 109-113 (1991). R-

Rolipram binds to the catalytic site of PDE4 see Torphyet al., Mol. Pharmacol., Vol. 39, pp. 376-384 (1991). Consequently, competition for [BH]R-rolipram binding provides an independent confirmation of the PDE4 inhibitor potencies of unlabeled competitors. The assay was performed at 30°C for 1 hr in 0.5! buffer containing (final concentrations): 50 mM Tris-HCI, pH 7.5, 5 mM MgCl,, 0.05% bovine serum albumin, 2 nM [3H]R-rolipram (56.7 x 104 dpm/pmol) and various concentrations of non-radiolabeled inhibitors. The reaction was stopped by the addition of 2.5 mi of ice-cold reaction buffer (without [SH}-R-rolipram) and rapid vacuum filtration (Brandel

Cell Harvester) through Whatman GF/B filters that had been soaked in 0.3% polyethylenimine. The filters were washed with an additional 7.5 mi of cold buffer, dried, and counted via liquid scintillation spectrometry.

The invention thus provides, in a further aspect, a combination comprising a . "30 compound of formula (I) or a physiologically acceptable solvate thereof together with a PDE4 inhibitor. ‘ The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical formulations comprising a combination as defined above together with a pharmaceutically acceptable diluent or carrier represent a further aspect of the invention.

The individual compounds of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations.

Appropriate doses of known therapeutic agents will be readily appreciated by those skilled in the art.

The compounds of formula (I) and solvates thereof may be prepared by the methodology described hereinafter, constituting a further aspect of this invention.

A process according to the invention for preparing a compound of formula (1) comprises alkylation of a thioacid of formula (ll) 0 SH

HO CH, _. OR, 0 00 (mn

R, wherein R? R® R*, R®and ~~ are as defined above.

In this process the compound of formula (li) may be reacted with, for example, an appropriate alkyl or haloalkyl halide under standard conditions.

When R; represents fluoromethyl, the preferred haloalkyl halide reagent is bromofluoromethane.

Compounds of formula (Il) may be prepared from the corresponding 17a- hydroxyl derivative of formula (lll): 0 SH oo

HO rs ° 0 (ny

Rs wherein R?, R®, RY, R®and ~ are as defined above, using for example, the methodology described by G. H. Phillipps et al, (1994) Journal of Medicinal

Chemistry, 37, 3717-3729. For example the compound of formula (If) may be reacted with a compound of formula R,COOH or an activated derivative thereof eg an activated ester, anhydride or halide (eg the acid chloride). The reaction may be performed in the presence of an organic solvent eg triethylamine, usually together with dimethylaminopyridine (DMAP).

Compounds of formula (Ill) may be prepared in accordance with procedures . described in GB 2088877B. Compounds of formula (lil) may also be prepared by a process comprising the following steps:

° OH © on o *

CH, CH, HO J 0 e0 wv 0 0 wv 0 e0 an

F F :

Step (a) comprises oxidation of a solution containing the compound of formula (IV). Preferably, step (a) will be performed in the presence of a solvent comprising methanol, water, tetrahydrofuran, dioxan or diethylene glygol dimethylether. For example, so as to enhance yield and throughput, preferred solvents are methanol, water or tetrahydrofuran, and more preferably are water or tetrahydrofuran, especially water and tetrahydrofuran as solvent. Dioxan and diethylene glygol dimethylether are also preferred solvents which may optionally (and preferably) be employed together with water. Preferably, the solvent will be present in an amount of between 3 and 10vol relative to the amount of the starting material (1wt.), more preferably between 4 and 6 vol., especially 5 vol. Preferably the oxidising agent is present in an amount of 1-9 molar equivalents relative to the amount of the starting material. For example, when a 50% w/w aqueous solution of periodic acid is employed, the oxidising agent may be present in an amount of between 1.1 and 10wt. relative to the amount of the starting material (1wt.), more preferably between 1.1 and 3wt., especially 1.3wt.

Preferably, the oxidation step will comprise the use of a chemical oxidising agent.

More preferably, the oxidising agent will be periodic acid or iodic acid or a salt thereof. Most preferably, the oxidising agent will be periodic acid or sodium periodate, especially periodic acid. Alternatively (or in addition), it will also be-appreciated-that-- the oxidation step may comprise any suitable oxidation reaction, eg. one which utilises air and/or oxygen. When the oxidation reaction utilises air and/or oxygen, the solvent used in said reaction will preferably be methanol. Preferably, step (a) will involve incubating the reagents at room temperature or a little warmer, say around 25 °C eg for 2 hours. The compound of formula (V) may be isolated by recrystallisation from the reaction mixture by addition of an anti-solvent. A suitable anti-solvent for compound of formula (V) is water. Surprisingly we have discovered that it is highly desirable to control the conditions under which the compound of formula (V) is precipitated by addition of anti-solvent eg water. When the recrystallisation is performed using chilled water (eg waterfice mixture at a temperature of 0-5 °C) although better anti-solvent properties may be expected we have found that the crystalline product produced is very voluminous, resembles a soft gel and is very difficult to filter. Without being limited by theory we believe that this low density product contains a large amount of solvated solvent within the crystal lattice By . contrast when conditions of around 10°C or higher are used (eg around ambient temperature) a granular product of a sand like consistency which is very easily . filtered is produced. Under these conditions, crystallisation typically commences after around 1 hour and is typically completed within a few hours (eg 2 hours). Without being limited by theory we believe that this granular product contains little or no of solvated solvent within the crystal lattice.

Step (b) will typically comprise the addition of a reagent suitable for converting a carboxylic acid to a carbothioic acid eg. using hydrogen sulphide gas together with a suitable coupling agent eg. carbonyldiimidazole (CDI) in the presence of a suitable solvent eg. dimethylformamide. : Solvates of compounds of formula (I) which are not physiologically acceptable may be useful as intermediates in the preparation of compounds of formula (I) or physiologically acceptable solvates thereof. :

The advantages of compounds of formula (I) and/or solvates thereof may include the fact that the substances appear to demonstrate excellent anti- inflammatory properties, with predictable pharmacokinetic and pharmacodynamic behaviour, with an attractive side-effect profile (demonstrated for example, by increased selectivity for the glucocorticoid receptor over the progesterone receptor and/or increased selectivity for glucocorticoid receptor mediated transrepression over transactivation) and are compatible with a convenient regime of treatment in human patients. Further advantages may include the fact that the substances have desirable physical and chemical properties which allow for ready manufacture and storage.

The following non-limiting Examples illustrate the invention:

EXAMPLES

General ) 30 LCMS was conducted on a Supelcosil LCABZ+PLUS column (3.3 cm x 4.6 mm ID) eluting with 0.1% HCO,H and 0.01 M ammonium acetate in water (solvent A), and ) 0.05% HCO,H 5% water in acetonitrile (solvent B), using the following elution gradient 0-0.7 min 0%B, 0.7-4.2 min 100%B, 4.2-5.3 min 0%B, 5.3-5.5 min 0%B at a flow rate of 3 ml/min. The mass spectra were recorded on a Fisons VG Platform spectrometer using electrospray positive and negative mode (ES+ve and ES-ve).

intermediates

Intermediate 1: 17a-(Cyclobutyicarbonyl)oxy- 6a.9a-difluoro-11B-hydroxy-16q- methyl-3-oxo-androsta-1.4-diene-1783-carbothioic acid

A solution of 6a,9a-difluoro-11p,17a-dihydroxy-16a-methyl-3-oxo-androsta-1,4- ) diene-17B-carbothioic acid (prepared in accordance with the procedure described in

GB 20888778) (1g, 2.42mmol) in anhydrous dichloromethane (20m) and - triethylamine (0.88ml, 6.32mmol) was treated at <5 °C under nitrogen with a solution of cyclobutanecarbonyl chloride (0.72mi, 6.31mmol) in anhydrous dichloromethane (5ml) over approximately 2min. The solution was stirred at <5 °C for 45min and then diluted with dichloromethane (20ml) and washed successively with 5% sodium hydrogen carbonate solution (20ml), 1M hydrochloric acid (20ml) and water (20mt).

The organic solution was dried (Na,SO,) and evaporated to give an off-white foam (1.479) which was dissolved in acetone (30ml) and treated with 1-methylpiperazine (1ml, 9mmol). After 2.5h the solution was slowly added to a stirred mixture of 2M hydrochloric acid (55ml) and ice (55ml) and the precipitate was collected and dried in vacuo to give the title compound as a white solid (1.12g, 93.5%): LCMS retention time 3.79min, m/z 495 MH"

Intermediate 2: 17a~(Cyclopentylcarbonyl)oxy- 6a, 9a-difluoro-11p-hydroxy-16a- : methyl-3-oxo-androsta-1,4-diene-178-carbothioic acid.

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 4.00min, m/z 509 MH*

Intermediate 3: 17a~(Cyclohexylcarbonyl)oxy- 6a. 9a-difluoro-11p-hydroxy-16a- methyl-3-oxo-androsta-1,4-diene-17-carbothioic acid .. . __Prepared using methods similar_to_that described for Intermediate 1. LCMS retention time 4.17min, m/z 523 MH"

Intermediate 4: 17a-(Cyclopropylcarbonyl)oxy- 6a,9a-difluoro-11B-hydroxy-16a- methyl-3-oxo-androsta-1.4-diene-173-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 3.65min, n/z 481 MH*

Intermediate 5. 6a.,9a-Difluoro-11p-hydroxy-16a-methyl-17a-(1- methycyclopropylcarbonyl)oxy-3-oxo-androsta-1.4-diene-173-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 3.75min, m/z 495 MH+

Intermediate 6: 6a. 9a-Difluoro-11p-hydroxy-16a-methyl-3-ox0-17a-(2,2.3.3- tetramethycyclopropylcarbonyl)oxy-androsta-1,4-diene-178-carbothioic acid

Prepared using methods similar to that described for Intermediate 1. : LCMS retention time 4.12min, m/z 537 MH*

Intermediate 7: 17a-(2,2-Dichloro-1-methycyclopropylcarbonyl)oxy-6o.9a-difluoro- ’ 11B-hydroxy-16a-methyl-3-oxo-androsta-1,4-diene-173-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 4.20min, m/z 563,565 MH* :

Intermediate 8: 17a-(2,2-Dichlorocylopropylcarbonyl)oxy-6a.9a-difluoro-11p- hydroxy-16a-methyl-3-oxo-androsta-1.4-diene-17B-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 4.14min, m/z 549,551 MH*

Intermediate 9: 6a,9a-Difluoro-11p-hydroxy-16a-methyl-17a-(3- methylenecyclobutylcarbonyl)oxy-3-oxo-androsta-1.4-diene-178-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 4.10min, m/z 507 MH*

Intermediate 10: 6a, 9a-Difluoro-118-hydroxy-16a-methyl-17o-(2- methyicyclopropyicarbonyl)oxy-3-oxo-androsta-1,4-diene-17B-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 3.90min, m/z 495 MH*

Intermediate 11: 6a, 9a-Difluoro-118-hydroxy-16a-methyl-17a-(1- methyicyclobutylcarbonyl)oxy-3-oxo-androsta-1,4-diene-17B-carbothioic acid

Prepared using methods similar to that described for intermediate 1.

LCMS retention time 4.13min, m/z 509 MH+

Intermediate 12: 6a, 9a-Difluoro-17a-(3,3-dimethylcyclobutyicarbonyl)oxy-118- hydroxy-16a-methyl-3-oxo-androsta-1.4-diene-17-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 4.09min, m/z 523 MH*

Intermediate 13: 6a,9a-Difluoro-17a-(3,3-difiuorocyclobutylcarbonyl)oxy-118- hydroxy-16a-methyl-3-oxo-androsta-1,4-diene-17p3-carbothioic acid . Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 3.78min, m/z 531 MH*

Intermediate 14: 6a.9a-Difluoro-113-hydroxy-16a-methyl-170-(1- methylcyclopentylcarbonyl)oxy-3-oxo-androsta-1.4-diene-17B-carbothioic acid

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 4.05min, m/z 523 MH+

Intermediate 15: 6a.,9a-Difluoro-118-hydroxy-16a-methyl-3-ox0-17a-(3- oxocyclobutylcarbonyl)oxy-androsta-1,4-diene-178-carbothioic acid ‘

Prepared using methods similar to that described for Intermediate 1.

LCMS retention time 3.41min, m/z 509 MH* ’

Examples

Example 1: 17a-(Cyclobutylcarbonyl)oxy- 6a.9a-difluoro-118-hydroxy-16a-methyl-3- oxo-androsta-1,4-diene-178-carbothioic acid S-fluoromethyl ester

Sodium hydrogen carbonate (112mg, 1.33mmol) was added to a solution of

Intermediate 1 (600mg, 1.21mmol) in anhydrous N,N-dimethylformamide (6ml) and the mixture cooled to —20 °C under nitrogen. Bromofiuoromethane (0.15mi, 2.7mmol) was added and the mixture was stirred at —20 °C for 2h. Diethylamine (0.6m, 5.8mmol) was added and the mixture stirred at —20 °C for 15min and then added to vigorously stirred 2M hydrochloric acid (25ml). Water (75ml) was added and after stirring for a further 30min the white precipitate was collected and dried in vacuo (606mg). This material was purified was column chromatography on silica gel to give the title compound as a white solid (520mg, 81%): LCMS retention time 3.67min, m/z 527 MH".

Example 2: 170-(Cyclopentylcarbonyloxy- 6a,9a-difluoro-118-hydroxy-16a-methyl- 3-oxo-androsta-1,4-diene-173-carbothioic acid S-fluoromethy! ester

Prepared from Intermediate 2 using methods similar to that described for Example 1

LCMS retention time 3.92min, m/z 541 MH* _..._ Example 3: 17a-(Cyclohexylcarbonyl)oxy- 6a,9a-difluoro-118-hydroxy-16a-methyl-3- oxo-androsta-1.4-diene-173-carbothioic acid S-fluoromethyt ester _.. _._Prepared from Intermediate 3 using methods similar to that described for Example 1 _

LCMS retention time 4.02min, m/z 555 MH*

Example 4: 17a-(Cyclopropylcarbonyl)oxy- 6a.9a-difluoro-11p-hydroxy-16a-methyi- 3-oxo-androsta-1,4-diene-178-carbothioic acid S-fluoromethyl ester

Prepared from Intermediate 4 using methods similar to that described for Example 1

LCMS retention time 3.54min, m/z 513 MH*

Example 5: 6a,9a-Difluoro-113-hydroxy-16a-methyl-17a-(1- methycyclopropylcarbonyl)oxy-3-oxo-androsta-1,4-diene-17p-carbothioic acid S- fluoromethyl ester

Prepared from Intermediate 5 using methods similar to that described for Example 1

LCMS retention time 3.66min, m/z 527 MH+

Example 6: 6a.9a-Difluoro-113-hydroxy-16a-methyl-3-oxo0-17a-(2,2.3,3- : tetramethycyclopropylcarbonyl)oxy-androsta-1.4-diene-178-carbothioic acid S- fluoromethyl ester ) Prepared from Intermediate 6 using methods similar to that described for Example 1

LCMS retention time 4.02min, m/z 569 MH*

Example 7: 17a-(2,2-Dichloro-1-methycyclopropylcarbonyl)oxy-6a.,9a-difluoro-118- hydroxy-16a-methyl-3-oxo-androsta-1.4-diene-173-carbothioic acid S-fluoromethyi ester

Prepared from Intermediate 7 using methods similar to that described for Example 1

LCMS retention time 3.79min, m/z 595,597,599 MH*

Example 8. 17a-(2,2-Dichlorocylopropylcarbonyl)oxy-6a.,9a-difluoro-113-hydroxy- 16a-methyl-3-oxo-androsta-1.4-diene-17B-carbothioic acid S-fluoromethy! ester

Prepared from Intermediate 8 using methods similar to that described for Example 1

LCMS retention time 3.68min, m/z 581,583 MH"*

Example 9: 6a.,9a-Difluoro-11p3-hydroxy-16a-methyl-17a-(3- methylenecyclobutyicarbonyl)oxy-3-oxo-androsta-1,4-diene-17p-carbothioic acid S- fluoromethyl ester

Prepared from Intermediate 9 using methods similar to that described for Example 1

LCMS retention time 3.68min, m/z 539 MH*

Example 10: 6a,9a-Difluoro-118-hydroxy-16a-methyl-17a-(2- methylcyclopropylcarbonyl)oxy-3-oxo-androsta-1,4-diene-173-carbothioic acid S- fluoromethyl ester

Prepared from Intermediate 10 using methods similar to that described for Example 1. LCMS retention time 3.57min, m/z 527 MH"

Example 11: 6a. 9a-Difluoro-11p-hydroxy-16a-methyl-17a-(1- methylcyclobutylcarbonyl)oxy-3-oxo-androsta-1,4-diene-17-carbothioic acid S- fluoromethyl ester

Prepared from [Intermediate 11 using methods similar to that described for Example 1. LCMS retention time 3.73min, m/z 541 MH*

Example 12: 6a,9a-Difluoro-17a-(3,3-dimethylcyclobutylcarbonyl)oxy-11p3-hydroxy- 16a-methyl-3-oxo-androsta-1.4-diene-178-carbothioic acid S-fluoromethyl ester

Water (1.8ml), benzyitributylammonium chloride (35mg) and diisopropylethylamine (0.21ml) were added to a stirred and cooled (0 °C) solution of Intermediate 12 (585mg, 1.12mmol) in ethyl acetate (15ml). A solution of bromofluoromethane (0.075ml) in ethyl acetate (0.75mi) was added and the mixture stirred at room : temperature for 18h. A solution of 2% diethylamine in 4:1 acetonitrile:water (1ml) was added and the mixture stirred for 10min. The organic phase was separated, washed successively with 0.5M hydrochloric acid, water and 1% sodium bicarbonte solution and dried and evaporated. The residue (570mg) was purified by preparative HPLC to give the title compound as a cream solid (490mg, 79%): LCMS retention time 3.75min, m/z 555 MH*. :

Example 13: 6a.9a-Difluoro-17a-(3,3-difluorocyclobutylcarbonyl)oxy-118-hydroxy- 16a-methyl-3-oxo-androsta-1.4-diene-17B-carbothioic acid S-fluoromethyl ester

Prepared from Intermediate 13 using methods similar to that described for Example 12. LCMS retention time 3.47min, m/z 563 MH’

Example 14: 6a,9a-Difluoro-118-hydroxy-16a-methyl-17a-(1- methylcyclopentylcarbonyl)oxy-3-oxo-androsta-1,4-diene-17B-carbothioic acid S- fluoromethy! ester

Prepared from Intermediate 14 using methods similar to that described for Example 12. LCMS retention time 3.71min, m/z 5565 MH*

Example 15: 6a,9a-Difluoro-118-hydroxy-16a-methyl-3-0x0-17 o-(3- oxocyclobutylcarbonyl)oxy-androsta-1,4-diene-173-carbothioic acid S-fluoromethyl ester

Prepared from Intermediate 15 using methods similar to that described for Example __ .._ 12. LCMS retention time 3.24min, m/z 541. MH* _

Preparation of long acting $_-adrenoreceptor agonist

Example X: 3-(4-{[6-({(2R)-2-Hydroxy-2-[4-hydroxy-3- _ (hydroxymethyl)phenyljethyl}amino)-hexyljoxy}butyl)benzenesulfonamide acetate i) Dictert-butyl) 2-(2,2-dimethyl-4H-1,3-benzodioxin-6-yl)-2-oxoethylimidodicarbonate

Cesium carbonate (70.4g) was added to a stirred suspension of 2-bromo-1-(2,2- dimethyl-4H-1,3-benzodioxin-6-yl)ethanone, (Glaxo, DE 3513885, 1985) (61.8g) and di-t-buty! iminodicarboxylate (47.159) in acetonitrile (600ml) under nitrogen. After vigorous stirring at 21°C for 24 h the mixture was diluted with water (ca800ml) and the product was extracted with diethyl ether (1litre, then 200ml). The combined organic layers were washed with brine, dried (MgSOQ,) and concentrated to ca400ml.

The white crystals were collected by filtration, washed with diethyl ether and dried to give the title compound (24.4g) 5 (CDCl) 7.78(1H, dd, J 8, 2Hz), 7.65 (1H, brs), 6.87(1H, d, J 8Hz), 4.97(2H, s), 4.88(2H, s), 1.56(6H, s) and 1.48 (18H, s) . Further concentration of the mother liquors gave additional product (13.8g). A third crop . (7.1g) was obtained by chromatographing the mother liquors on silica gel, 5S evaporating the appropriate eluate and triturating with diethyl ether. . ii) tert-Butyl 2-(2,2-dimethyl-4H-1,3-benzodioxin-6-yl)-2-oxoethylcarbamate

Trifluoroacetic acid (92ml) was added to a stirred solution of di(tert-butyl) 2-(2,2- dimethyl-4H-1,3-benzodioxin-6-yl)-2-oxoethylimidodicarbonate, (352.55q) in dichloromethane (3.6litres) at 21°C and the reaction was stirred for 1.5 h. Aqueous

NaOH solution (1.75litres) was added and after 10 min the phases were separated.

The organic layer was washed with water, dried (MgSO,) and evaporated to an oil.

This was stored under high vacuum overnight and then triturated with hexane:ether (3:1) to give the crude product (226.61g). This was purified by recrystallisation from diethy! ether to give the title compound (122.78g). Further product (61.5g) was obtained from the mother liquors by evaporation and chromatography on a Biotage using 15% ethyl acetate in hexane. LCMS RT = 3.37min. iii) tert-Butyl (2R)-2-(2.2-dimethyl-4H-1 ,3-benzodioxin-6-yl)-2-hydroxyethylcarbamate

A 2M solution of borane - dimethyl sulphide in THF (28ml) was added slowly to a 1M solution of (R)-tetrahydro-1-methyl-3,3-diphenyl-1H,3H-pyrrolo[1,2- c][1,3,2]oxazaborole in toluene (56ml) at 0°C under nitrogen. A solution of tert-butyl 2-(2,2-dimethyl-4H-1,3-benzodioxin-6-yl)-2-oxoethylcarbamate, (108.2g) in THF (1.3litres) was added slowly keeping the temperature below 5°C followed by 2M solution of borane - dimethyl sulphide in THF (252ml) over 50 min. After 1 h, 2M HCI (170ml) was added with cooling and the mixture was partitioned between ethyl acetate and water. The organic layer was washed with saturated NaHCO, solution and brine and dried (MgSO,). The solution was concentrated and the product purified by chromatography on flash silica gel (800g), eluting successively with hexane:ethyl acetate (4:1 then 3:1) to give the title compound (93.3g), LCMS RT = 3.31min. . 30 iv) (5R)-5-(2,2-Dimethyl-4H-1,3-benzodioxin-6-yl)-1,3-oxazolidin-2-one tert-Butyl (2R)-2-(2,2-dimethyl-4H-1,3-benzodioxin-6-yl)-2-hydroxyethylcarbamate, . (86.379) in DMF (600ml) was added dropwise to a stirred suspension of sodium hydride (60% oil dispersion, 11.9g) in DMF (160ml) with cooling such that the internal temperature remained at 0°C under nitrogen. The mixture was stirred at 21°C for 2 h. The mixture was recooled to 0°C and 2M HCI (134ml) was added. The mixture

Claims (35)

1. A compound of formula (I) . o R,

0 . HO CH, og, CH, gf Rs J wherein R; represents C, alkyl or C,_; haloalkyl; R, represents C,4 cycloalkyl or C,4 cycloalkenyl either of which may optionally be substituted by one or more groups selected from oxo, methyl, methylene and halogen; RR; represents hydrogen, methyl (which may be in either the « or B configuration) or methylene; R, and R; are the same or different and each represents hydrogen or halogen; and “= represents a single or a double bond; and solvates thereof. 2 A compound according to claim 1 in which R, represents C, ; cycloalkyl or C, cycloalkenyl either of which may optionally be substituted by one or more groups selected from methyl, methylene and halogen.

3. A compound according to claim 1 or claim 2 in which R, represents C,, cycloalkyl optionally substituted by one or more methyl and/or chlorine groups.

4, A compound according to any one of claims 1 to 3 in which R, represents fluoromethyl, chloromethyl, bromomethy! or 2’-fluoroethyl.

5. A compound according to claim 4 in which R, represents fluoromethyl. .

6. A compound according to any one of claims 1 to 5 in which R, is methyl.

7. A compound according to any of claims 1 to 6 in which R, and R; are the . same or different and each represents hydrogen, fluorine or chlorine.

8. A compound according to claim 7 in which R, and R; are the same or different and each represents hydrogen or fluorine.

9. A compound according to claim 8 in which both R, and R; are fluorine.

10. A compound according to any one of claims 1 to 9 in which == represents a double bond.

11. A compound according to claim 1 which is: 170-~(Cyclobutylcarbonyl)oxy-6o., Sa-difluoro-11 B-hydroxy-16a-methyl-3-oxo- androsta-1,4-diene-17B-carbothioic acid S-fluoromethy| ester; 170-~(Cyclopentylcarbonyl)oxy- 6a, 9a-difluoro-11p-hydroxy-1 6a-methyl-3-oxo- androsta-1,4-diene-17-carbothioic acid. S-fluoromethyl ester; 17a-(Cyclohexyicarbonyl)oxy- 6a,90-difluoro-11p-hydroxy-1 6a-methyl-3-oxo- androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester; 17a-(Cyclopropylcarbonyl)oxy- 6c, 9a-difluoro-1 1B-hydroxy-16a-methyl-3-oxo- androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; or a solvate of any one thereof.

12. A compound according to claim 1 which is: 6a, 9a-Difluoro-11p-hydroxy-16a-methyl-170-(1 -methycyclopropylcarbonyt) oxy-3-oxo-androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl! ester; 6a, 9a-Difluoro-11p-hydroxy-16a-methyl-3-oxo-170-(2,2,3,3- tetramethycyclopropylcarbonyl)oxy-androsta-1,4-diene-173-carbothioic acid S- fluoromethyl ester; 17a~(2,2-Dichloro-1-methycyclopropylcarbonyl)oxy-6a, 9a-difluoro-11 B-hydroxy-16a- methyl-3-oxo-androsta-1,4-diene-178-carbothioic acid S-fluoromethy! ester; 17a-(2,2-Dichlorocylopropylcarbonyl)oxy-6a,9a-difluoro-11p-hydroxy-1 6a-methyl-3- oxo-androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester; 6a,9a-Difluoro-11p-hydroxy-16a-methyl-1 7a-(3-methylenecyclobutylcarbonyl)oxy-3- oxo-androsta-1,4-diene-17B-carbothioic acid S-fluoromethyl ester; 6a,9a-Difluoro-118-hydroxy-1 Ba-methyl-17a-~(2-methylcyclopropylcarbonyl)oxy-3- oxo-androsta-1,4-diene-1 7B-carbothioic acid S-fluoromethyl ester; or a solvate of any one thereof.

13. A compound according to claim 1 which is: 6a,9a-Difluoro-11B-hydroxy-16a-methyl-17a-(1 -methylcyclobutylcarbonyl)oxy-3-oxo- androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester: or a solvate of any one thereof.

14. A compound according to claim 1 which is: 6a,9a-Difluoro-1 7a~(3,3-dimethylcyclobutylcarbonyl)oxy-11B-hydroxy-16a-methyl-3- oxo-androsta-1,4-diene-17-carbothioic acid S-fluoromethyl ester; 60, 9a-Difluoro-17a-(3,3-difluorocyclobutylcarbonyl)oxy-118-hydroxy-1 6a-methyl-3- oxo-androsta-1,4-diene-17p-carbothioic acid S-fluoromethy! ester; } 6a, 9a-Difluoro-11p-hydroxy-16a-methyl-17a-(1-methylcyclopentylcarbonyl)oxy-3- oxo-androsta-1,4-diene-17p-carbothioic acid S-fluocromethyl ester; 60, 9a-Difluoro-11pB-hydroxy-16a-methyl-3-oxo-17a-(3-oxocyclobutylcarbonyl)oxy- androsta-1,4-diene-17p-carbothioic acid S-fluoromethyl ester; or a solvate of any one thereof.

15. A compound of formula (I) as defined in any of claims 1 to 14 or a physiologically acceptable solvate thereof for use in veterinary or human medicine.

16. Use of a compound of formula (I) as defined in any of claims 1 to 14 or a physiologically acceptable solvate thereof for the manufacture of a medicament for the treatment of inflammatory and/or allergic conditions.

17. A pharmaceutical composition comprising a compound of formula (1) as defined in any of claims 1 to 14 or a physiologically acceptable solvate thereof together, if desirable, in admixture with one or more physiologically acceptable diluents or carriers.

18. A pharmaceutical aerosol formulation comprising a compound of formula (1) as defined in any of claims 1 to 14 or a physiologically acceptable solvate thereof, and a fluorocarbon or hydrogen-containing chlorofluoro carbon as propellant, optionally in combination with a surfactant and or a cosolvent.

19. A pharmaceutical composition according to claim 17 which further comprises another therapeutically active agent.

20. A pharmaceutical composition according to claim 19 in which said another therapeutically active agent is a B,-adrenoreceptor agonist.

21. A pharmaceutical composition according to claim 20 in which said p,- ’ 30 adrenoreceptor agonist is a compound of formula (X):

PCT/GB02/01971 HOCH, R" i oo oO prmrceou{( 0

H . 13 or a salt or solvate thereof, wherein: mis an integer of from 2to0 8; ’ nis an integer of from 3 to 11, with the proviso that m + nis 5to 19, : Ris ~XSO,NR™R" wherein X Is ~(CH,),- or C,, alkenylene; - R' and R" are independently selected from hydrogen, C,salkyl, Csscycloalkyl, C(O)NR'™®R", phenyl, and phenyl (C_alkyl)-, or R* and R", together with the nitrogen to which they are bonded, form a 5-, 6, or 7- membered nitrogen containing ring, and R'® and R" are each optionally substituted by one or two groups selected from halo, C, qalkyl, C,shaloalkyl, Csalkoxy, hydroxy- substituted C, alkoxy, -CO,R'™, -SO,NR'R", .CONR"R"™, -NR™C(O)R™, or a 5-, 6- or 7-membered heterocylic ring; R* and Rare independently selected from hydrogen, C, alkyl, Csetycloalkyl, phenyl, and phenyl! (Cy.alkyl)~; and p is an integer of from 0 to 8, preferably from 0 to 4; R' and R*® are independently selected from hydrogen, C, alkyl, Cysalkoxy, halo, phenyl, and Cy haloalkyl: and : R" and R'® are independently selected from hydrogen and C..qalkyl with the proviso that the total number of carbon atoms in R™ and R'® is not more than 4.

22. A pharmaceutical composition according to claim 21 in which the compound of formula (X) Is 3-(4-{I8~({(2R)-2-Hydroxy-2-[4-hydroxy-3- (hydroxymethyl)phenyllethyl}amino)-hexylJoxy}butyl)benzenesulfonamide or a salt or solvate thereof.

23. A substance or composition for use in a method for the treatment of a : human or animal subject with .an anti-inflammatory and/or allergic condition, said substance or composition comprising a compound of formula (I) as defined in any of claims 1.to 14 or a physiologically - 30 acceptable solvate thereof, and said method comprising administering an effective amount of said substance or composition to said human or animal subject.

24. A process for preparing a compound of formula (1) according to claim 1 which comprises alkylation of a compound of formula (I) AMENDED SHEET

On _SH Ho CH, “ok, fy gf Re om foo : OR : wherein R%, R®, RY, Rand = gre as defined In claim 1.

25. A process according to claim 24 wherein alkylation is performed by reacting the compound for formula (1 with an appropriate alkyl! or haloalkyl halide. >

26. A compound of formula (Il) On SH Ho CH, oA, CH, gf Ry m AIL Ry : wherein R?, R®, RY, R®and "are as defined in claim 1.

27. Use of a compound of formula (I) as defined in any of claims 1 to 14 or a physiologically acceptable solvate thereof in the manufacture of a medicament for treatment of human or animal.

28. | A substance or composition for use in a method of treatment of human or animal, said substance or composition comprising a compound of formula (I) as defined in any of .claims 1 to 14 or a physiologically acceptable solvate thereof, and said method comprising administering said substance or composition. : : AMENDED SHEET

’ PCT/GB02/01971

29. A compound according to any one of claims 1, or 26, substantially as herein described and illustrated.

30. A substance or composition for use in a method of treatment according to any one of claims 15, or 23, or 28, substantially as herein described and illustrated.

31. Use according to any one of claims 16, or 27, substantially as herein described and illustrated.

32. A composition according to claim 17, substantially as herein described and illustrated.

33. A formulation according to claim 18, substantially as herein described and illustrated.

34. A process according to claim 24, substantially as herein described and illustrated.

35. A new compound, a substance or composition for a new use in a method of treatment, a new use of a compound of formula (I} as defined in any of claims 1 to 14 or of a physiologically acceptable solvate thereof, a new composition, a new formulation, or a new process for preparing a compound, substantially as herein described. AMENDED SHEET