ZA200303474B

ZA200303474B - Compositions for antitumour treatment containing Ecteinascidin 743.

Info

Publication number: ZA200303474B
Application number: ZA200303474A
Authority: ZA
Inventors: Naoto Takahashi; Maurizio D Incalci; Rafaella Giavazzi; Steve Weitman; Glynn Thomas Faircloth; Andreas Gescher
Original assignee: Pharma Mar Sa
Priority date: 2000-11-06
Filing date: 2003-05-06
Publication date: 2004-08-06
Also published as: UA79926C2; SI1365808T1; ES2358787T3

Description

Effective Antitumour Treatments

The present invention relates to effective antitamour treatments.

Ecteinascidin 743, ET743, is an anticancer agent derived from a marine source.

BACKGROUND OF THE INVENTION

The reader is referred to WO0069441 published 23 November 2000 for information on compositions and uses of ET743 for treating cancer. This text is incorporated by reference.

SUMMARY OF THE INVENTION :

In accordance with one aspect of this invention, we provide effective combination therapies based on ecteinascidin 743, using other drugs.

PREFERRED EMBODIMENTS

The other drugs may form part of the same composition, of be provided as a separate composition for administration at the same time or a different time. The identity of the other drug is not particularly limited, and suitable candidates include: a) drugs with antimitotic effects, especially those which target cytoskeletal elements, including microtubule modulators such as taxane drugs (such as taxol, paclitaxel, taxotere, , docetaxel), podophylotoxins or vinca alkaloids (vincristine, vinblastine); b) antimetabolite drugs such as 5-fluorouracil, cytarabine, gemcitabine, purine analogues such as pentostatin, methotrexate); aE c) alkylating agents such as nitrogen mustards (such as cyclophosphamide or ifosphamide); . d) drugs which target DNA such as the antracycline drugs adriamycin, doxorubicin, pharmorubicin or epirubicin, : ’ e) drugs which target topoisomerases such as etoposide; f) hormones and hormone agonists or antagonists such as estrogens, antiestrogens (tamoxifen and related compounds) and androgens, flutamide, leuprorelin, goserelin, cyprotrone or octreotide; 2 drugs which target signal transduction in tumour cells including antibody derivatives such as herceptin; h) alkylating drugs such as platinum drugs (cis-platin, carbonplatin, oxaliplatin, paraplatin) or nitrosoureas; i) drugs potentially affecting metastasis of tumours such as matrix metalloproteinase inhibitors;

I); gene therapy and antisense agents; k) antibody therapeutics;

D other bioactive compounds of marine origin, notably the didemnins such as aplidine; m) steroid analogues, in particular dexamethasone; n) anti-inflammatory drugs, in particular dexamethasone; and 0) anti-emetic drugs, in particular dexamethasone.

As patt of this patent specification, we include a series of examples and now refer to them.

These examples demonstrate the increased effectiveness of ET-743 when used in combination with other drugs and are concerned with different combinations using ET- 743.

Example 1 relates to effective combinations of ET-743 and doxorubicin for tumour . growth inhibitions against marine and human sarcomas in athymic mice. ‘ Example 2 shows ecteinascidin 743 (ET-743) and doxorubicin produce synergistic cytotoxic effects in soft tissue sarcoma lines HT-1080 and HS-18.

These two examples show more than additive effects of the combination of ET-743 with anthracyclines (in particular doxorubicin) which is more effective than either alone against . human tumours (in these specific experiments sarcoma), which effects occur independent of sequence of administration. Such results show clear promise for treatment of patients.

Example 3 shows a synergistic cytoxic effect of ET-743 and cisplatin.

Example 4 provides a sequencing evaluation of ET-743 in combinations with chemotherapy agents against a panel of human tumour cell lines, in particular ET743 combinations with doxorubicin, taxol, SN-38, cisplatin, and gemcitabine.

These two show more than additive effects of the combination of ET-743 with platinum antitumour compounds, (in particular Cis-platin) with the nucleoside analogue gemcitabine, and with an inhibitor of topoisomerase IT (SN38, which is the active agent produced from pro-drug CPT-11, a drug of the camptothecin group). Again these combinations are more effective than either drug alone against human tumours (in these specific experiments against a variety of tumour cells: ovarian, colon, lung, breast, bone sarcoma), which effects were dependent on sequence of exposure in some cases. Again there is promise for treatment of patients.

Interestingly, synergistic action was cleatly not predictable: Example 4 indicates that in most combinations tested, no synergy was observed (in fact, antagonism was reported in some cases).

Example 5 relates to evaluation of combinations of Et-743 with doxorubicin or trimetrexate or paclitaxel.

It shows more than additive effects of the combination of ET-743 with anthracyclines (in : particular doxorubicin) which is more effective than either alone against human tumours (in these specific experiments sarcoma), which effects occur independent of sequence of ' administration. Such results show clear promise for treatment of patients.

s

Examples 6 to 8 reinforce and complement the previous examples, and especially show the synergy of ET-743 and doxorubicin and also ET-743 with cisplatin.

Example 9 demonstrates a different kind of effective ness of the combinations of this ) invention, where high-dose dexamethasone protects against the hepatotoxicity of ecteinascidin-743 (ET-743).

In summary, this invention therefore provides compositions, methods of treatment, processes for preparing compositions and related embodiments.

The present invention also extends to the compounds of the invention for use in 2 method of treatment, and to the use of the compounds in the preparation of a composition for treatment of cancer.

Thus, the present invention provides a method of treating any mammal, notably a human, affected by cancer which comprises administering to the affected individual a therapeutically effective amount of a compound of the invention, or a pharmaceutical composition thereof.

The present invention also relates to pharmaceutical preparations including a pharmaceutically acceptable carrier, which contain as active ingredient a compound or compounds of the invention, as well as the processes for their preparation.

Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, : granules, etc.) or liquid (solutions, suspensions or emulsions) with suitable composition or oral, topical or parenteral administration, and they may contain the pure compound or in combination with any carrier or other pharmacologically active compounds. These compositions may need to be sterile when administered parenterally.

Administration of the compounds or compositions of the present invention may be by any ! suitable method, such as intravenous infusion, oral preparations, intraperitoneal and intravenous administration. We prefer that infusion times of up to 24 hours are used, more preferably 2-12 hours, with 2-6 hours most preferred. Short infusion times which allow treatment to be carried out without an overnight stay in hospital are especially desirable. However, infusion may be 12 to 24 hours or even longer if required. Infusion . may be carried out at suitable intervals of say 2 to 4 weeks. Pharmaceutical compositions containing compounds of the invention may be delivered by liposome or nanosphere ’ encapsulation, in sustained release formulations or by other standard delivery means.

The correct dosage of the compounds will vary according to the particular formulation, the mode of application, and the particular sifu, host and tumour being treated. Other factors like age, body weight, sex, diet, time of administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease shall be taken into account. Administration can be carried out continuously or periodically within the maximum tolerated dose.

The combinations of this invention can be used on refractory patients. The reader is referred to WO0069441 for information on dosing schemes for ET-743 and other information of use in the combination therapy of this invention.

EXAMPLES OF THE INVENTION

Example 1

Effective Combinations Of Et-743 And Doxorubicin For Tumor Growth Inhibitions

Against Murine And Human Sarcomas In Athymic Mice

ET-743 has confirmed clinical activity in patients with soft and bone sarcoma refractory to previous chemotherapy including Doxorubicin (Dx) and Isosfamide. In view of the potential clinical value in combining ET-743 with Dx we have investigated this : combination against the murine fibrosarcoma UV2237, its mdr-resistant subline

UV2237/ADR and the human rabdomyosarcoma zenograft TE671. Both ET743 and Dx ' alone were effective against murine UV2237 fibrosarcoma whereas each was inactive or marginally active against both UV2237/ADR and TE671. However, the combination of

ET743 and Dx was effective in all 3 models. The synergism was particularly marked in the human rabdomyosarcoma TE671 and appeared independent of drug sequence or combination.

After single i.v. treatments performed when the tumor TE671 was approximately of 100 } mg tumor weight inhibition (TWI) and Log 10 Cell Kill (LCK) values were respectively 46% and 0.132 for ET-743 (0.1 mg/kg) alone, 50% and 0.33 for Dx (10 mg/kg) alone, 77% and 0.924 for ET-743 (0.1 mg/kg) and Dx (10 mg/kg) given simultaneously, 82% and 1.12 for the combination of ET-743 (0.1 mg/kg) given 1 hour before Dx (10 mg/kg) and 75% and 0.85 for the combination of ET-743 (0.1 mg/kg) given 1 h after Dx (10 mg/kg).

These data suggest that the combination of ET-743 and Dx can also be effective in tumors that are not sensitive or marginally sensitive to these drugs given alone, thus providing a strong rationale for clinical investigations using this combination.

Example 2

Ecteinascidin 743 (et-743) and Doxorubicin Produce Synergistic Cytotoxic Effects in Soft

Tissue Sarcoma Lines HT-1080 and HS-18.

Two sarcoma cell lines, HT 1080, a fibrosarcoma cell line sensitive to ET-743 (ICso= 10pm) and HS-18, a liposarcoma cell line, less sensitive to ET-743 (ICs0 = 270pm) were evaluated for toxicity to ET-743 in combination with either doxorubicin, trimetrexate or paclitazel. When ET-743 was used in combination with each of these drugs at a constant molar ration, and analysed by the method of Chou and Talalay, synergistic effects were obtained (72 hr incubation) with the ET-743-doxorubicin combination, but not with the combination of ET-743 with trimetrexate or paclitaxel. When cells were exposed to ET- 743 for 72 hr, and either doxorubicin, tritnetrexate ot taxol for the last 48 hrs of . incubation, synergistic effects were also obtained with doxorubicin against both sarcoma cell lines. Of interest, the sequence paclitaxel followed by ET-743 was more effective than ' the opposite sequence. These results encourage clinical trials of doxorubicin in combination with ET-743 to treat patients with soft tissue sarcoma, as both of these drugs have shown activity against this disease.

: Example 3

Synergistic Cytotoxic Effect Of Et-743 And Cisplatin

Ecteinascidin 743 (ET-743) has shown striking antitumor activity in several preclinical systems and promising clinical activity. ET-743 binds N2 guanines in the minor groove and affects the regulation of transcription (Minuzzo et al., PNAS, Vol. 97,6780-84, 2000).

Previous studies have indicated that mismatch repair (MMR) deficient cells are equally sensitive to ET-743 as proficient cells. NER deficient cells very sensitive to cisplatin are 6- 8 times less sensitive to ET-743. On the basis of the different mechanisms involved in the tepair of ET-743 and cisplatin and because of the potential clinical interest in this combination we have performed studies to evaluate the cytotoxic effects of ET-743 and cisplatin in several human tumor cell lines. Human ovarian cancer Igrove-1 cell line, a subline resistant to ET-743 aG/ PSC/ ET), human colon cancer HCT 116, (MMR deficient) and HCT11-ch3 (MMR proficient) cell lines were used in this study.

The cells were treated for 1 or 24 h with different concentrations of ET-743 or cisDDP, alone or in combinations, and the cytotoxicity was evaluated by using a colorimetric assay after sulforodhamine B staining. In all the cell lines a synergistic effect was observed both with 1 h or 24 h exposure. Interestingly in HCT116 resistant to cisDDP ET-743 was apparently able to reverse sensitivity even at concentrations of ET-743 which alone were marginally effective. Taken together the data provide a tational for undertaking clinical studies combining ET-743 with cisDDP. : Example 4

Et743 Combinations With Doxorubicin, Taxol, Sn-38, Cisplatin, And Gemcitabine

ET-743 was evaluated in combination with doxorubicin, taxol, SN-38, cisplatin, and gemcitabine against a panel of human tumor cell lines. These studies were designed to . determine the type of drug-drug interaction between ET-743 and standard chemotherapy agents and the influence of sequence of exposure on antitumor activity. Multiple : combinations of ET-743 with standard cytotoxic agents were used with a model-free design (Laska, ef al. Biometrics 50:834, 1994) to describe the type of drug-drug interaction.

These studies suggest that regardless of exposure, an additive pattern of drug-drug interaction is most typically observed.

A synergistic drug-drug interaction was observed when ET-743 was combined against non- small cell lung (pre-exposure to SN-38), osteosarcoma (pre-exposure with ET-743 followed by cisplatin), breast (pre-exposure to ET-743 followed by gemcitabine), colon (pre- exposure with ET-743 followed by SN-38 and concurrent exposure with SN-38) tumor cell lines. An additive/synergistic (pre-exposure to ET-743 followed by SN-38 against NSCL; pre-exposure to SN-38 against colon and NSCL; concurrent exposure with cisplatin against osteosarcoma, and with SN-38 against NSCL lines) pattetn of drug-drug interaction was observed. Evidence of antagonism was noted when taxol was utilized concurrently against two NSCL lines, and doxorubicin against a thabomyosarcoma cell line.

These studies suggest that ET-743 which is in Phase II clinical trials, could be combined with several cytotoxic agents against a broad-range of tumor types.

Material And Methods

Cell culture:

Human breast (MDA-435, MDA-231, T-470), non-small cell lung (NCI-H522, NC1-H226,

NCI-H23), colon (HCT-116, HT-29, Colo-320), osteosarcoma (HOS, U-2, OS, Sa08-2), : rhabdomyosarcoma (RH1, RH30, RD) tumor cell lines were grown in RPMI-1640 supplemented with 10% fetal bovine serum and 2mM L-glutamine. All stock cultures were maintained in 75 cm-2 flasks at 37°C in humidified incubators with 2 5% CO2-95% air atmosphere. :

ICs Analysis: : A pre-determined number of exponentially growing tumor cells were inoculated in 96-well tissue culture plates and allowed to stabilize for 24 hours. Afterwards, a drug plate consisting of serial diluted concentrations of ET-743 or standard chemotherapy agents was added to the cells. Cells were incubated as a 24-hour exposure for three days followed by the addition of MTT for 4 hours. Resultant formazan crystals were then solubilized with acid/ alcohol, with absorbance (570 nm-test/630 nm-reference) determined using a microplate reader. Results were expressed as percent tumor cell kill compared to media controls.

Combination Studies:

For the combination studies, the concentration (expressed as a percent of the individual agent’s ICsp) schema used to characterize the type of interaction is shown below:

Drug Concentration (Expressed as a percent of the ICs)

ET-743 Standard agents

EL pe

Statistical Analysis of Combination Studies:

Statistical comparisons are made with each test combination (75:25-ET-743/standard agents) and the endpoints (100:0-ET-743 and 0:100-standard agents). A statistically significant observation requires that a difference exists between the combination (ET-743 and standard agents) absorbance value and both endpoint values (ET-743 and standard agents alone). If the majority of (>3 of 5) the values are statistically above or below the line then antagonism or synergy is described, respectively. Otherwise the pattern is more : consistent with an additive interaction. Interpretation is very difficult if there is considerable slope to the line connecting the endpoints. If the slope of the ICsp curves for the individual agents are identical (unlikely) then you can, at times, determine the type of interaction.

Tumor Exposure Conditions/ Agents Drug-Drug Interactions

Cw

NOS 24 hour ET-743 followed by 24 hour Synergistic exposure to cisplatin 24 hour cisplatin followed by 24 hour Additive exposure to ET-743 24 hour concurrent ET-743/ cisplatin Additive exposure

U2-0S8 24 hour ET-743 followed by 24 hour Additive exposure to cisplatin 24 hour cisplatin followed by 24 hour Additive exposure to ET-743 24 hour concurrent ET-743 / cisplatin Additive exposute

Sa06 24 hour ET-743 followed by 24 hour Additive exposure to cisplatin 24 hour cisplatin followed by 24 hour Additive exposure to ET-743 24 hour concurrent ET-743 / cisplatin Additive/Synergistic exposure

Non-Small Cell il HE 24 hour ET-743 followed by 24 hour Additive

I a

24 hour taxol followed by 24 hour Additive

NCB-H226 exposure to ET-734 24 hour concurrent ET-743/ taxol Antagonistic exposure ' 24 hour ET-743 followed by 24 hour Additive/ Synergistic exposure to SN38 24 hour SN-38 followed by 24 hour Additive/ Synergistic exposure to ET-743 24 hour concurrent ET-743/SN-38 Additive exposure 24 hour ET-743 followed by 24 hour Additive exposure to taxol

NCB-N522 24 hour taxol followed by 24 hour Additive exposure to ET-734 24 hour concurrent ET-743/taxol Antagonistic exposure 24 hour ET-743 followed by 24 hour Additive/Synergistic exposure to SN38 24 hour SN-38 followed by 24 hour Additive/ Synergistic exposure to ET-743 24 hour concurrent ET-743/SN-38 Additive exposure 24 hour ET-743 followed by 24 hour Additive/ Antagonistic exposure to taxol

NCB-N23 24 hour taxol followed by 24 hour Additive exposure to ET-734 24 hour concurrent ET-743/taxol Antagonistic exposure 24 hour ET-743 followed by 24 hour Additive exposure to SN38 24 hour SN-38 followed by 24 hour Synergistic exposure to ET-743 24 hour concurrent ET-743/SN-38 Additive/Synergistic i WO 02/36135 PCT/GB01/04902 eee

EI I ER

' MDA-435 24 hour ET-743 followed by 24 hour Additive exposure to gerncitabine 24 hour gemcitabine followed by 24 hour | Additive exposure to ET-743 : 24 hour concurrent ET-473 /germcitabine | Additive

MDA-231 24 hour ET-743 followed by 24 hour Additive exposure to gerncitabine 24 hour gemcitabine followed by 24 hour | Additive exposure to ET-743 24 hour concurrent ET-473 /germcitabine | Additive 24 hour ET-743 followed by 24 hour Additive

T47-8 exposure to gerncitabine 24 hour gemcitabine followed by 24 hour | Additive exposure to ET-743 24 hour concurrent ET-473 /germcitabine | Additive

MCT-116 24 hour ET-743 followed by 24 hour Synergistic exposure to SN-38 24 hour ET-743 followed by 24 hour Additive exposure to SN-38 24 hour concurrent ET-743/SN exposure | Additive

NT-29 24 hour ET-743 followed by 24 hour Additive exposure to SN-38 24 hour ET-743 followed by 24 hour Additive exposure to SN-38 24 hour concurrent ET-743/SN exposure | Additive ’ Colo-320 24 hour ET-743 followed by 24 hour Additive exposure to SN-38 24 hour ET-743 followed by 24 hour Additive/Synergistic exposure to SN-38 24 hour concurrent ET-743/SN exposure | Synergistic :

thabdomyo- = : RN1 24 hour ET-743 followed by 24 hour Additive exposure doxorubicin } 24 hour doxorubicin followed by 24 hour | Additive exposure to ET-743 24 hour concurrent ET-743 /doxorubicin | Antagonistic exposure 24 hour ET-743 followed by 24 hour Additive exposure doxorubicin 24 hour doxorubicin followed by 24 hour | Additive exposure to ET-743 24 hour concurrent ET-743/doxorubicin | Additive/Antagonistic exposure

RN30 24 hour ET-743 followed by 24 hout Additive exposure doxorubicin 24 hour doxorubicin followed by 24 hour | Additive exposure to ET-743 24 hour concurrent ET-743 /doxorubicin Antagonistic exposure

Conclusions-Summary

These studies suggest that regardless of sequence of exposure between ET-743 and standard chemotherapy agents, an additive pattern of drug-drug interaction is most typically observed. ’ Evidence of synergy was observed when NC1-H522 and NC1-H23 NSCL lines were pre- exposed to SN-38, pre-exposure to ET-743 with cisplatin against HOS osteosarcoma, T- ) 470 breast cell line with gemcitabine, SN-38 against HCT-116 colon, and concurrent exposure with SN-38 against Colo-320 colon tumor cell line.

Evidence of antagonism was observed when taxol was utilized concurrently against the

NC1-H226 and NC1-H23 NSCL cell line and doxorubicin against the RHI , rhabdomyosarcoma tumor cell line.

Example 5

Interaction Between Ft-743 And Other Antineoplastic Agents

Although ET-743 is presently in clinical trials from human cancers, the mechanisms of antitumor activity of ET-743 have not been completely elucidated. The aim of this study was to assess the nature of the interaction between ET-743 and other antineoplastic agents (doxorubicin; DXR, trimetrexate; TMTX and Paclitaxel; Taxol) using the combination index (CI) method of Chou and Talalay. To better understand how ET-743 might be used clinically, the present study used SRB assays to examine the cytotoxicity resulting from combining ET-743 with three other antineoplastic agents in the different administration schedules in two soft tissue sarcoma cell lines, HT-1080 and HS-18, in vitro. DXR was the only agent that resulted in sequence-independent synergy when combined with ET- 743. Concurrent exposure of ET-743 with DXR resulted in synergistic interactions in both cells lines.

The Cs (mean) with the schedule were 0.86, 0.83, 0.84 and 0.85 at 50, 75, 90 and 95% cell kill, respectively, in HT-1080 cells and 0.89, 0.74, 0.64 and 0.60 at 50, 75, 90 and 95% cell kill, respectively, in HS-18 cells. Sequencing with ET-743 for 24 h prior to DXR was the most effective regimen against both cell lines; it resulted in consistently low CI of up to the about 90% cell kill level for both cell lines. Exposure to Taxol prior to ET-743 was also an effective regimen. These results suggest that the combination of ET-743 and DXR should be explored further in clinical trials in the treatment of soft tissue sarcoma.

Materials And Methods

Chemicals

ET-743 was provided by Pharma-Mar S.A (Tres Cantos, Madrid, Spain), and was prepared as a 2 mM stock solution in dimethyl sulfoxide. Paclitaxel and DXR were obtained from . Sigma chemical Co. (St. Louis, MO). TMTX was supplied by Warner-Lambert (Parke-

Davis, Ann Arbor, Mich).

Cell Culture

Soft tissue sarcoma cell lines, HT-1080 and HS-18 were maintained as monolayer cultures in RP<I-1640 containing 10% fetal bovine serum.

SRB Cytotoxicity Assay

Cytotoxicity to drugs was determined by SRB cytotoxicity assay carried out in 96-well microtiter plates as described. Cells were plated in duplicate wells (5000 cells/well) and exposed to drugs at different concentrations. Cells were fixed with 50% TCA solution for 1 h and 0.4% SRB (Sigma) was added to each well. After a 30 min incubation, the plate were washed with 1% acetic acid and read at 570 nm on a Biowhitaker microplate reader 2001. The wells with cells containing no drugs and with medium plus drugs but no cells were used as positive and negative controls, respectively.

Concurrent Exposure to ET-743 and DXR, TMTX or Paclitaxel

Cells were seeded into 96-well plates, as described previously. Cells were treated with seven different concentrations of the single drugs or combinations mixture at 1:100 (ET- 743 : the other drugs) molar ratio. After 72 h exposure, growth inhibition was measured using the SRB assay.

Sequential Exposure to ET-743 and DXR, TMTX or Paclitaxel

Using the same experimental setup described above, we exposed cells to three different ) concentrations of drugs which represents the ICas, ICso, ICs of ET-743, DXR, TMTX and paclitaxel, respectively. After 24 hours pre-treatment with ET743 or the combination drug, the second drugs were added to the respective wells for 48 h. Growth inhibition was determined using the SRB assay.)

Cell Cycle Analysis

Exponentially growth cells were treated with or without drugs for several hours. Cells were then collected and fixed with ice-cold 70% methanol. DNA was stained with propidium iodide as described previously. Ten thousand stained cells were analyzed on a

Becton Dickinson fluorescence-activated cell sorter (FACS).

Determination of Synergism and Antagonism and Construction of Isobolograms

The CI was calculated by the Chou-Talalay equation, which takes into account both potency (Dm or ICso) and the shape of the dose effect curve (the m value). The general equation for the classic isobologram (CI = 1) is given by:

CI= ©) / D1 + D)2/ O32 @ where (Dx); and (Dx) in the denominators are the doses (or concentrations) for D1 (ET- 743) and D> (another drug) alone that give X % inhibition, whereas (D)1 and (D)2 in the numerators are doses of ET-743 and another drug in combination also inhibited X % (ie isoeffective). CI < 1, CI = 1, CI > 1 indicated synergism, additive effect and antagonism, respectively. :

The (Dx)1 or (Dx)2 can be readily calculated from the median-effect equation of Chou and

Chou et al:

Dx = Dm [fa / (1 — fa)] V/m B) where Dm is the median-effect dose that is obtained from the anti-log of the X-intercept of the median-effect plot, X-log (D) versus Y — log [fa / (1 — fa)] or

Dm = 10 (intercept) / mand m is the slope of median-effect plot. Computer software of

Chou and Chou allows automated calculation of m, Dm, Dx, and CI values. From (Dm), (Dx)2, and D1 + D2, it becomes easy to constract isobolograms automatically based on Eq,

A.

For conservative mutually nonexclusive isobolograms of two agents, a third term,

(D1) (D2) / (DX): (DX): | © is added to Eq. A.

For simplicity, the third term is usually omitted, and thus the mutually exclusive ’ assumption or classic isobologram is indicated. In Result 2 and 3, the CI values obtained from the classic (mutually exclusive) calculation are given.

Result 1

Cytotoxicity of four drugs on HT-1080 and S18

TT] ICso for human soft tissue sarcoma cells

This table showed that both HT-1080 and S18 cell lines were more sensitive to E'T-743 than other antineoplasic agents.

Effect of each agent on cell cycle distribution against HS-18 cells 24 h and 72 h after treatment with approximate ICs; dose

LE Lc

ET-743 270 pM 24 32.4 47.6 20.0 72 86.7 8.4 4.9

DXR 225 aM 24 10.1 64.9 25.0 72 1.3 63.8 34.9

TMTX 70 uM 24 44.2 53.8 1.9 72 35.5 57.6 7.0

IE CC EN EI

‘ Effect of each agent on cell cycle distribution against HT-1080 cells ’ 24 h and 72 h after treatment with approximate ICso dose

Go [ws ww —

ET-743 10 pM 24 42.6 36.1 21.3

Nl

DXR 25 nM 24 36.1 17.5 46.4

J wl

TMTX 6 nM 24 31.9 56.8 11.3

J al I

Paclitaxel 1.3 nM 24 45.4 37.3 17.3

Bl CF

Result 2 shows the CI for HT-1080 and HS-18 cells, respectively, which were simultaneously exposed to ET-743 and one of antineoplastic drugs, such as DXR, TMTX or paclitaxel, at 1 to 100 molar ratio combination mixture. When cells were treated with

ET-743 and DXR, the CI values were all below 1, indicating synergism effect in both cell lines. The CI (mean) with this schedule were 0.86, 0.83, 0.84 and 0.85 at 50, 75, 90 and 95% cell kill, respectively, in HT-1080 cells and 0.89, 0.74, 0.64 and 0.60 at 50, 75, 90 and 95% cell kill, respectively, in HS-18 cells. This result showed that concurrent treatment of

ET-743 and DXR produced synergistic sytotoxic effect. In contrast, when cells were treated with ET-0743 and TMTX or paclitaxel, antagonism cytotoxic effect was observed.

The CI plot was obtained from both cell lines which were initially exposed to ET-743 fot 24 h, followed by DXR for 48 h. In both cells lines, ET-743 followed by DXR treatment showed synergistic cytotoxic effect, the CI value of HT-1080 at 80% cell kill level was 0.64 + 0.12 and that of HS-18 at 88% cell kill level was 0.24 + 0.06. In contrast, DXR followed by ET-743 treatment (Result 3a, lower figure) demonstrated the good CI value at first sight however, the CI value of HT-1080 at 80% cell kill level was 1.00 + 0.03, indicating that the effect of the two agents were additive, in addition, the CI at highest fraction killed was worse than that at middle fraction killed in both cells.

When cells were exposed to ET-743 followed by TMTX, the CI values of HT-1080 showed nearly one or over one, indicating that the effect of the two agents are antagonism or additive. In contrast, those of HS-18 were all under 0.6, demonstrating that these two drugs have synergy effect. When cells were treated with TMTX followed by ET-743, additive effect was observed in both HT-1080 and HS-18 cell lines.

Paclitaxel followed by ET-743 treatment produced synergistic cytotocix effect. When cells were exposed to paclitaxel followed by ET-743, the CI value of HT-1080 at 89% cell kill level was 0.92 + 0.06 and that of HS-18 at 78% cell kill level was 0.38 + 0.13.

Summary

ET-743 was highly active against human soft tissue sarcoma cells, especially against the malignant fibrosarcoma cell line HT-1080.

DXR resulted in sequence-independent synergy when combined with ET-743, however, sequencing with ET-743 followed by DXR was more effective against both cell lines.

Exposure to paclitaxel followed by ET-743 was also an effective regimen against human soft tissue sarcoma cells, while concomitant exposure was antagonistic.

Example 6

In vivo combinations of chemotherapeutic agents with Ecteinascidin 743 (Et743) against solid tumors.

Several unique mechanisms of action have been described for Et743 including binding to the minor groove of DNA, alkylation of the N2 of guanine, transcriptional inhibition of

MDR1 gene (Jin et. al, PNAS 97, 6775, 2000; Minuzzo et. al., PNAS 97, 6780, 2000) and

Claims

24 PCT/GB01/04902 CLAIMS

1. The use of ET-743 in the preparation of a medicament for an effective treatment by combination therapy employing ET-743 in synergistic combination with another anti- tumour drug.

2. The use of an anti-tumour drug in the preparation of a medicament for an effective treatment of a tumour by combination therapy employing the anti-tumour drug in synergistic combination with ET-743.

3. The use according to claim 1 or 2 where the ET-743 forms part of the same medicament.

4. The use according to claim 1 or 2, wherein the ET-743 is provided as a separate medicament.

5. The use according to claim 4, wherein the separate medicament containing ET- 743 is for administration at the same time as the medicament containing the anti-tumour drug.

6. The use according to claim 4, wherein the separate medicament containing ET- 743 is for administration at a different time as the medicament containing the anti-tumour drug.

7. The use according to any preceding claim, wherein the combination therapy employs ET-743 and an anthracycline.

8. The use according to claim 7, wherein the combination therapy employs ET-743 and doxorubicin.

9. The use according to any one of claims 1 to 6, wherein the combination therapy employs ET-743 and a platinum anti-tumour drug. AMENDED SHEET

PCT/GB01/04902

10. The use according to claim 9, wherein the platinum anti-tumour drug is cisplatin or carboplatin.

I1. The use according to any of claims 1 to 6, wherein the combination therapy employs ET-743 and a taxane anti-tumour drug.

12. The use according to claim 11, wherein the taxane anti-tumour drug is paclitaxel or docetaxel.

13. The use according to any of claims 1 to 6, wherein the combination therapy employs ET-743 and an antimetabolite anti-tumour drug.

14. The use according to claim 13, wherein the antimetabolite anti-tumour drug is gemcitabine or 5-fluorouracil.

15. The use according to any of claims 1 to 6, wherein the combination therapy employs ET-743 and a topoisomerase-inhibitor anti-tumour drug.

16. The use according to claim 15, wherein the topoisomerase-inhibitor anti-tumour drug is SN-38.

17. The use according to any preceding claim, wherein the combination therapy further employs dexamethasone.

18. A substance or composition for use with another anti-tumour drug in a method for an effective treatment of a tumour by combination therapy, said substance or composition comprising ET-743, and said method comprising administering said substance or composition in synergistic combination with said another anti-tumour drug.

19. A substance or composition for use with ET-743 in a method for an effective treatment of a tumour by combination therapy, said substance or composition comprising an anti-tumour drug, and said method comprising administering said substance or AMENDED SHEET

26 PCT/GB01/04902 composition in synergistic combination with said ET-743.

20. A substance or composition for use in a method of treatment according to claim 18 or 19, where the ET-743 forms part of said substance or composition.

21. A substance or composition for use in a method of treatment according to claim 18 or 19, wherein the ET-743 is provided as a separate substance or composition.

22. A substance or composition for use in a method of treatment according to claim 21, wherein the separate substance or composition containing ET-743 is for administration at the same time as the substance or composition containing the anti- tumour drug.

23. A substance or composition for use in a method of treatment according to claim 21, wherein the separate substance or composition containing ET-743 is for administration at a different time as the substance or composition containing the anti- tumour drug.

24. A substance or composition for use in a method of treatment according to any one of claims 18 to 23, wherein the combination therapy employs ET-743 and an anthracycline.

25. A substance or composition for use in a method of treatment according to claim 24, wherein the combination therapy employs ET-743 and doxorubicin.

26. A substance or composition for use in a method of treatment according to any of claims 18 to 23, wherein the combination therapy employs ET-743 and a platinum anti- tumour drug.

217. A substance or composition for use in a method of treatment according to claim 26, wherein the platinum anti-tumour drug is cisplatin or carboplatin. AMENDED SHEET

27 PCT/GB01/04902

28. A substance or composition for use in a method of treatment according to any of claims 18 to 23, wherein the combination therapy employs ET-743 and a taxane anti- tumour drug.

29. A substance or composition for use in a method of treatment according to claim 28, wherein the taxane anti-tumour drug is paclitaxel or docetaxel.

30. A substance or composition for use in a method of treatment according to any of claims 18 to 23, wherein the combination therapy employs ET-743 and an antimetabolite anti-tumour drug.

31. A substance or composition for use in a method of treatment according to claim 30, wherein the antimetabolite anti-tumour drug is gemcitabine or 5-fluorouracil.

32. A substance or composition for use in a method of treatment according to any of claims 18 to 23, wherein the combination therapy employs ET-743 and a topoisomerase- inhibitor anti-tumour drug.

33. A substance or composition for use in a method of treatment according to claim 32, wherein the topoisomerase-inhibitor anti-tumour drug is SN-38.

34. A substance or composition for use in a method of treatment according to any one of claims 18 to 33, wherein the combination therapy further employs dexamethasone.

35. Use according to any one of claims 1 to 17, substantially as herein described and illustrated.

36. A substance or composition for use in a method of treatment according to any one of claims 18 to 34, substantially as herein described and illustrated.

37. A new use of ET-743 and/or an anti-tumour drug; or a substance or composition for a new use in a method of treatment; substantially as herein described. AMENDED SHEET