WO2024180048A3 - Methods and devices for preparing capped rna by fed-batch transcription - Google Patents

Methods and devices for preparing capped rna by fed-batch transcription Download PDF

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Publication number
WO2024180048A3
WO2024180048A3 PCT/EP2024/054929 EP2024054929W WO2024180048A3 WO 2024180048 A3 WO2024180048 A3 WO 2024180048A3 EP 2024054929 W EP2024054929 W EP 2024054929W WO 2024180048 A3 WO2024180048 A3 WO 2024180048A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
devices
fed
capped rna
rna
Prior art date
Application number
PCT/EP2024/054929
Other languages
French (fr)
Other versions
WO2024180048A2 (en
Inventor
Alexander VAN ASBECK
Original Assignee
Ribopro B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ribopro B.V. filed Critical Ribopro B.V.
Publication of WO2024180048A2 publication Critical patent/WO2024180048A2/en
Publication of WO2024180048A3 publication Critical patent/WO2024180048A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to ribonucleic acid (RNA), and to methods for synthesizing and co-transcriptionally capping RNA. The invention is especially concerned with methods for enhanced co-transcriptional capping of mRNA using cap analogues. The invention also relates to apparatus for performing such methods, and to devices that enable the improved synthesis of in vitro transcribed mRNA, and more specifically to the addition of a 5' cap structure to in vitro transcribed mRNA.
PCT/EP2024/054929 2023-02-28 2024-02-27 Rna WO2024180048A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2302887.1 2023-02-28
GBGB2302887.1A GB202302887D0 (en) 2023-02-28 2023-02-28 Rna

Publications (2)

Publication Number Publication Date
WO2024180048A2 WO2024180048A2 (en) 2024-09-06
WO2024180048A3 true WO2024180048A3 (en) 2025-02-27

Family

ID=85794061

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/054929 WO2024180048A2 (en) 2023-02-28 2024-02-27 Rna

Country Status (2)

Country Link
GB (1) GB202302887D0 (en)
WO (1) WO2024180048A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006004648A1 (en) * 2004-06-29 2006-01-12 Ambion, Inc. Methods and compositions for preparing capped rna
WO2020185811A1 (en) * 2019-03-11 2020-09-17 Modernatx, Inc. Fed-batch in vitro transcription process
WO2021212034A1 (en) * 2020-04-16 2021-10-21 Nature's Toolbox, Inc. In vitro manufacturing and purification of therapeutic mrna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7074596B2 (en) 2002-03-25 2006-07-11 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Synthesis and use of anti-reverse mRNA cap analogues

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006004648A1 (en) * 2004-06-29 2006-01-12 Ambion, Inc. Methods and compositions for preparing capped rna
WO2020185811A1 (en) * 2019-03-11 2020-09-17 Modernatx, Inc. Fed-batch in vitro transcription process
WO2021212034A1 (en) * 2020-04-16 2021-10-21 Nature's Toolbox, Inc. In vitro manufacturing and purification of therapeutic mrna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PREGELJC DOMEN ET AL: "Increasing yield of in vitro transcription reaction with at-line high pressure liquid chromatography monitoring", BIOTECHNOLOGY AND BIOENGINEERING, vol. 120, no. 3, 15 December 2022 (2022-12-15), Hoboken, USA, pages 737 - 747, XP093109180, ISSN: 0006-3592, Retrieved from the Internet <URL:https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.28299> DOI: 10.1002/bit.28299 *

Also Published As

Publication number Publication date
WO2024180048A2 (en) 2024-09-06
GB202302887D0 (en) 2023-04-12

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